MD00G1026300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013723
Physical Location & Seq
Reverse (-)
4484770 .. 4486033
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1026300.v1.1.491

Sequence Viewer

Length: 882 bp
ATGGAGCACACTGTCCAGGAAAGTGATCCTGGTTCCCGCCATGAGGGGAAAGGCAAGGAAAGAGCTGGCAGTGTCCCGTGGAAGGACTTGAGGGTTGCCACGCGGCCAAAGGATTTTGGGGATATCAACAATTGCTTGGCAGGGCGTCGATTCGCCTTCGATGAGCTCGGAGAGCCCTTAGCTAAGGATGAATCGGATTGCGACCGGATGTTGAAGCTGTCTTCATATGTCATGGCCGAGTATCACGACAGACTGCAAGAGGTTGAGCGGTACAAGGCAAAACTGAAGGAGAATAAGCAGCTTGTGGACGAGGCCCGAAGGAATAAGGGACTTTTGACTCAGGCTCTCCAACTGAAGGACGAAACCATGGAGAGCTTGAAAAGGCGAAATGGTGAGAACCTAAGGCTTAAGAAATTGTTTGAGGCAACTAAAAAACAGTTGGAGGTGGCTACCTTGGAGGTATCCAAGGTTAGGGGAGAATTGGATGGTGCCTTAGTTGAGATTTCTGAACTGGAGAAGAGCATTCCAACTGAAAGGGAGGCTGCTGTGCAAGAATACTTAAGTTCTTCGACCTTTCATCTTGCTATTAAACCCTACTGTGCTCAAGAAGCTCGCTTTGAAAAAAGGAAATGGATGGCCGTCCTTGATCGTTATGATGATGGGAGCATTCTTCGAAAATACCACGAAGATATAGATGAGCATCATCGAAAGGGCGAGACATTTGTCCTTGCTGTTGATCCTAGCAGCGAAGATGAGTCTGATAATGAAGGTAGTGCTGATGCACAGACTCAGCATGGTGAAGAGGATCTTGGGGATGCAGAGGATGATGGTAGGACGCGGAGTGATACTGCCAGGGGTTCGGCTTCAGATGAGAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

33.35

Weight (kDa)

5.08

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 488
AccBSI CCGCTC 1 cut(s) 268
AccII CGCG 2 cut(s) 103, 838
AciI CCGC 4 cut(s) 37, 103, 268, 838
AclWI GGATC 3 cut(s) 20, 731, 813
AcoI YGGCCR 3 cut(s) 104, 234, 636
AcuI CTGAAG 3 cut(s) 305, 374, 849
AcyI GRCGYC 1 cut(s) 145
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 4 cut(s) 43, 82, 355, 471
AflII CTTAAG 2 cut(s) 407, 559
AgsI TTSAA 3 cut(s) 214, 379, 620
AjnI CCWGG 3 cut(s) 15, 28, 851
AjuI GAANNNNNNNTTGG 2 cut(s) 792, 824
AluBI AGCT 7 cut(s) 65, 166, 182, 217, 301, 375, 611
AluI AGCT 7 cut(s) 65, 166, 182, 217, 301, 375, 611
Alw21I GWGCWC 3 cut(s) 9, 168, 604
Alw26I GTCTC 1 cut(s) 710
AlwI GGATC 3 cut(s) 20, 731, 813
AoxI GGCC 4 cut(s) 104, 234, 312, 636
ApeKI GCWGC 3 cut(s) 298, 542, 744
AspS9I GGNCC 1 cut(s) 313
AsuHPI GGTGA 2 cut(s) 404, 809
AsuII TTCGAA 1 cut(s) 673
AxyI CCTNAGG 1 cut(s) 401
BanI GGYRCC 1 cut(s) 488
BanII GRGCYC 2 cut(s) 168, 177
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 3 cut(s) 9, 168, 604
BbvI GCAGC 3 cut(s) 310, 529, 756
BccI CCATC 4 cut(s) 479, 628, 653, 821
BceAI ACGGC 1 cut(s) 623
BciT130I CCWGG 3 cut(s) 17, 30, 853
BciVI GTATCC 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 710
BfaI CTAG 1 cut(s) 741
BfrI CTTAAG 2 cut(s) 407, 559
BfuI GTATCC 1 cut(s) 472
BisI GCNGC 4 cut(s) 104, 299, 543, 745
BlsI GCNGC 4 cut(s) 105, 300, 544, 746
Bme1390I CCNGG 3 cut(s) 17, 30, 853
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 2 cut(s) 34, 490
BmrFI CCNGG 3 cut(s) 17, 30, 853
BmsI GCATC 3 cut(s) 709, 769, 805
BoxI GACNNNNGTC 1 cut(s) 722
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 2 cut(s) 178, 183
Bpu14I TTCGAA 1 cut(s) 673
BpuEI CTTGAG 2 cut(s) 109, 588
BsaBI GATNNNNATC 1 cut(s) 699
BsaHI GRCGYC 1 cut(s) 145
BsaJI CCNNGG 5 cut(s) 77, 366, 453, 465, 852
BsaWI WCCGGW 1 cut(s) 204
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 82, 355, 471
Bse1I ACTGG 1 cut(s) 516
Bse21I CCTNAGG 1 cut(s) 401
Bse8I GATNNNNATC 1 cut(s) 699
BseBI CCWGG 3 cut(s) 17, 30, 853
BseDI CCNNGG 5 cut(s) 77, 366, 453, 465, 852
BseGI GGATG 6 cut(s) 193, 213, 490, 639, 820, 829
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 4 cut(s) 43, 82, 355, 471
BseMII CTCAG 2 cut(s) 353, 803
BseNI ACTGG 1 cut(s) 516
BseXI GCAGC 3 cut(s) 310, 529, 756
Bsh1236I CGCG 2 cut(s) 103, 838
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 4 cut(s) 106, 236, 314, 638
BshNI GGYRCC 1 cut(s) 488
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 3 cut(s) 9, 168, 604
BsiSI CCGG 1 cut(s) 205
BslFI GGGAC 2 cut(s) 59, 342
BslI CCNNNNNNNGG 4 cut(s) 43, 82, 355, 471
BsmAI GTCTC 1 cut(s) 710
BsmFI GGGAC 2 cut(s) 59, 342
BsmI GAATGC 2 cut(s) 522, 666
BsnI GGCC 4 cut(s) 106, 236, 314, 638
Bsp119I TTCGAA 1 cut(s) 673
Bsp1286I GDGCHC 4 cut(s) 9, 168, 177, 604
Bsp143I GATC 4 cut(s) 25, 646, 736, 805
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 4 cut(s) 37, 103, 268, 838
BspANI GGCC 4 cut(s) 106, 236, 314, 638
BspCNI CTCAG 2 cut(s) 352, 802
BspFNI CGCG 2 cut(s) 103, 838
BspLI GGNNCC 2 cut(s) 34, 490
BspPI GGATC 3 cut(s) 20, 731, 813
BspQI GCTCTTC 1 cut(s) 512
BspT104I TTCGAA 1 cut(s) 673
BspT107I GGYRCC 1 cut(s) 488
BspTI CTTAAG 2 cut(s) 407, 559
BsrBI CCGCTC 1 cut(s) 268
BsrI ACTGG 1 cut(s) 516
BssECI CCNNGG 5 cut(s) 77, 366, 453, 465, 852
BssMI GATC 4 cut(s) 25, 646, 736, 805
BssNI GRCGYC 1 cut(s) 145
BssT1I CCWWGG 3 cut(s) 366, 453, 465
Bst2UI CCWGG 3 cut(s) 17, 30, 853
Bst4CI ACNGT 3 cut(s) 13, 438, 599
Bst6I CTCTTC 2 cut(s) 512, 795
BstACI GRCGYC 1 cut(s) 145
BstAFI CTTAAG 2 cut(s) 407, 559
BstBI TTCGAA 1 cut(s) 673
BstC8I GCNNGC 2 cut(s) 67, 613
BstDEI CTNAG 6 cut(s) 178, 183, 339, 401, 493, 789
BstDSI CCRYGG 2 cut(s) 77, 366
BstF5I GGATG 6 cut(s) 193, 213, 490, 639, 820, 829
BstFNI CGCG 2 cut(s) 103, 838
BstKTI GATC 4 cut(s) 28, 649, 739, 808
BstMAI GTCTC 1 cut(s) 710
BstMBI GATC 4 cut(s) 25, 646, 736, 805
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 2 cut(s) 172, 608
BstNI CCWGG 3 cut(s) 17, 30, 853
BstPAI GACNNNNGTC 1 cut(s) 722
BstSCI CCNGG 3 cut(s) 15, 28, 851
BstUI CGCG 2 cut(s) 103, 838
BstV1I GCAGC 3 cut(s) 310, 529, 756
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
Bsu36I CCTNAGG 1 cut(s) 401
BsuI GTATCC 1 cut(s) 472
BsuRI GGCC 4 cut(s) 106, 236, 314, 638
BtgI CCRYGG 2 cut(s) 77, 366
BtsCI GGATG 6 cut(s) 193, 213, 490, 639, 820, 829
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 9, 76
Cac8I GCNNGC 2 cut(s) 67, 613
Cfr13I GGNCC 1 cut(s) 313
CseI GACGC 2 cut(s) 134, 844
Csp6I GTAC 1 cut(s) 271
CviAII CATG 4 cut(s) 41, 232, 367, 794
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 6 cut(s) 178, 183, 339, 401, 493, 789
DpnI GATC 4 cut(s) 27, 648, 738, 807
DpnII GATC 4 cut(s) 25, 646, 736, 805
EaeI YGGCCR 3 cut(s) 104, 234, 636
Eam1104I CTCTTC 2 cut(s) 512, 795
EarI CTCTTC 2 cut(s) 512, 795
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 3 cut(s) 366, 453, 465
Eco24I GRGCYC 2 cut(s) 168, 177
Eco32I GATATC 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 3 cut(s) 305, 374, 849
Eco81I CCTNAGG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 166
EcoRII CCWGG 3 cut(s) 15, 28, 851
EcoRV GATATC 1 cut(s) 124
EcoT14I CCWWGG 3 cut(s) 366, 453, 465
EcoT38I GRGCYC 2 cut(s) 168, 177
ErhI CCWWGG 3 cut(s) 366, 453, 465
FaeI CATG 4 cut(s) 44, 235, 370, 797
FaiI YATR 8 cut(s) 42, 226, 228, 233, 368, 654, 692, 795
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 2 cut(s) 59, 342
FatI CATG 4 cut(s) 40, 231, 366, 793
FauI CCCGC 1 cut(s) 44
FauNDI CATATG 1 cut(s) 226
Fnu4HI GCNGC 4 cut(s) 104, 299, 543, 745
FokI GGATG 6 cut(s) 200, 220, 497, 646, 827, 836
FriOI GRGCYC 2 cut(s) 168, 177
Fsp4HI GCNGC 4 cut(s) 104, 299, 543, 745
FspBI CTAG 1 cut(s) 741
GluI GCNGC 4 cut(s) 104, 299, 543, 745
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 4 cut(s) 106, 236, 314, 638
HapII CCGG 1 cut(s) 205
HgaI GACGC 2 cut(s) 134, 844
Hin1I GRCGYC 1 cut(s) 145
Hin1II CATG 4 cut(s) 44, 235, 370, 797
HinfI GANTC 5 cut(s) 150, 191, 337, 755, 787
HpaII CCGG 1 cut(s) 205
HphI GGTGA 2 cut(s) 404, 809
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 5 cut(s) 170, 196, 508, 760, 868
Hpy188III TCNNGA 2 cut(s) 245, 605
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 1 cut(s) 150
HpyAV CCTTC 6 cut(s) 76, 166, 280, 312, 349, 761
HpyCH4III ACNGT 3 cut(s) 13, 438, 599
HpyCH4V TGCA 4 cut(s) 256, 550, 782, 818
HpyF10VI GCNNNNNNNGC 2 cut(s) 172, 608
HpyF3I CTNAG 6 cut(s) 178, 183, 339, 401, 493, 789
Hsp92I GRCGYC 1 cut(s) 145
Hsp92II CATG 4 cut(s) 44, 235, 370, 797
Kzo9I GATC 4 cut(s) 25, 646, 736, 805
LguI GCTCTTC 1 cut(s) 512
LmnI GCTCC 2 cut(s) 4, 663
Lsp1109I GCAGC 3 cut(s) 310, 529, 756
LweI GCATC 3 cut(s) 709, 769, 805
MaeI CTAG 1 cut(s) 741
MalI GATC 4 cut(s) 27, 648, 738, 807
MbiI CCGCTC 1 cut(s) 268
MboI GATC 4 cut(s) 25, 646, 736, 805
MboII GAAGA 7 cut(s) 213, 529, 558, 662, 698, 761, 812
MfeI CAATTG 1 cut(s) 130
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 4 cut(s) 9, 168, 177, 604
MluCI AATT 3 cut(s) 130, 413, 479
MlyI GAGTC 3 cut(s) 331, 764, 781
MmeI TCCRAC 3 cut(s) 373, 420, 551
MseI TTAA 3 cut(s) 408, 560, 588
MspCI CTTAAG 2 cut(s) 407, 559
MspI CCGG 1 cut(s) 205
MspR9I CCNGG 3 cut(s) 17, 30, 853
MunI CAATTG 1 cut(s) 130
Mva1269I GAATGC 2 cut(s) 522, 666
MvaI CCWGG 3 cut(s) 17, 30, 853
MvnI CGCG 2 cut(s) 103, 838
MwoI GCNNNNNNNGC 2 cut(s) 172, 608
NcoI CCATGG 1 cut(s) 366
NdeI CATATG 1 cut(s) 226
NdeII GATC 4 cut(s) 25, 646, 736, 805
NlaIII CATG 4 cut(s) 44, 235, 370, 797
NlaIV GGNNCC 2 cut(s) 34, 490
NmeAIII GCCGAG 1 cut(s) 262
NspV TTCGAA 1 cut(s) 673
PciSI GCTCTTC 1 cut(s) 512
PctI GAATGC 2 cut(s) 522, 666
PfeI GAWTC 2 cut(s) 150, 191
PfoI TCCNGGA 1 cut(s) 15
PkrI GCNGC 4 cut(s) 105, 300, 544, 746
PleI GAGTC 3 cut(s) 331, 763, 781
PpsI GAGTC 3 cut(s) 331, 763, 781
PshAI GACNNNNGTC 1 cut(s) 722
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 3 cut(s) 15, 28, 851
PspGI CCWGG 3 cut(s) 15, 28, 851
PspN4I GGNNCC 2 cut(s) 34, 490
PspPI GGNCC 1 cut(s) 313
PsuI RGATCY 1 cut(s) 805
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SacI GAGCTC 1 cut(s) 168
SapI GCTCTTC 1 cut(s) 512
SaqAI TTAA 3 cut(s) 408, 560, 588
SatI GCNGC 4 cut(s) 104, 299, 543, 745
Sau3AI GATC 4 cut(s) 25, 646, 736, 805
Sau96I GGNCC 1 cut(s) 313
SchI GAGTC 3 cut(s) 331, 764, 781
ScrFI CCNGG 3 cut(s) 17, 30, 853
SduI GDGCHC 4 cut(s) 9, 168, 177, 604
SfaNI GCATC 3 cut(s) 709, 769, 805
SfuI TTCGAA 1 cut(s) 673
SmlI CTYRAG 4 cut(s) 88, 407, 559, 603
SmoI CTYRAG 4 cut(s) 88, 407, 559, 603
Sse9I AATT 3 cut(s) 130, 413, 479
SsiI CCGC 4 cut(s) 37, 103, 268, 838
SspMI CTAG 1 cut(s) 741
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 3 cut(s) 15, 28, 851
StyI CCWWGG 3 cut(s) 366, 453, 465
TaaI ACNGT 3 cut(s) 13, 438, 599
TaqI TCGA 5 cut(s) 148, 159, 569, 673, 706
TasI AATT 3 cut(s) 130, 413, 479
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 2 cut(s) 150, 191
Tru1I TTAA 3 cut(s) 408, 560, 588
Tru9I TTAA 3 cut(s) 408, 560, 588
TscAI CASTG 2 cut(s) 16, 76
TseI GCWGC 3 cut(s) 298, 542, 744
TspDTI ATGAA 4 cut(s) 204, 213, 566, 780
TspRI CASTG 2 cut(s) 16, 76
Vha464I CTTAAG 2 cut(s) 407, 559
XspI CTAG 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.