MD14G1153500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013723
Physical Location & Seq
Forward (+)
24765559 .. 24768088
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1153500.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGTGGATGTCAGAGCCTGGAAGTTCTATGACGCGGGGCACCATCCAACTGTTTGGGCAGGAGCTATCTGACATAGAGAAGGTGTTGAGGGTGCCCAAAGAGGATAGACACTTAAGCAAGCTACGACCCTTATTTCGTCGGTACGGTTTTCAACCCTTAGTTTCCGAGAGCCAGGGACGATCGATGGAGAAGGTAAGCAAGAAAACAGGGACTAGCACCCATAAAAGGAAAGCACCAGTGTTAGTTCCTTCGGAAGACATCCTACCGCATAAGAAAATTCATAAGTTCCGAGGGGAACCATCCGTTAGACCTAAGTCCCAAGATGGGGTCCTTAAGGGGCCTGCCTTTAGGAAGACTGGAGTCGAGACCGTTGATAATGCTACTGCCGTAGTTGCAGGAGAAGGGAGCCGACTGTTGCCTCATCCTCTTACTATGGAGCACACTGTCCAGGAAAGTGATCCTGGTTCCCGCCATGAGGGGAAAGGCAAGGAAAGAGCTGGCAGTGTCCCGTGGAAGGACTTGAGGGTTGCCACGCGGCCAAAGGATTTTGGGGATATCAACAATTGCTTGGCAGGGCGTCGATTCGCCTTCGATGAGCTCGGAGAGCCCTTAGCTAAGGATGAATCGGATTGCGACCGGATGTTGAAGCTGTCTTCATATGTCATGGCCGAGTATCACGACAGACTGCAAGAGGTTGAGCGGTACAAGGCAAAACTGAAGGAGAATAAGCAGCTTGTGGACGAGGCCCGAAGGAATAAGGGACTTTTGACTCAGGCTCTCCAACTGAAGGATGAAACCATGGAGAGCTTGAAAAGGCGAAATGGTGAGAACCTAAGGCTTAAGAAATTGTTTGAGGCAACTAAAAAACAGTTGGAGGTGGCTACCTTGGAAGTATCCAAGGTTAGGGGAGAATTGGATGGTGCCTTAGTTGAGATTTCTGAACTGGAGAAGAGCATTCCAACTGAAAGGGAGGCTGCTGTGCAAGAATACTTAAGTTCTTCGACCTTTCATCTTGCTATTAAACCCTACTGTGCTCAAGAAGCTCGCTTTGAAAAAAGGAAATGGATGGCCGTCCTTGATCGTTATGATGATGGGAGCATTCTTCGAAAATACCACGAAGATATAGATGAGCATCATCGAAAGGGCGAGACATTTGTCCTTGCTGTTGATCCTAGCAGCGAAGATGAGTCTGATAATGAAGGTAGTGCTGATGCACAGACTCAGCATGGTGAAGAGGATCTTGGGGATGCAGAGGATGATGGTAGGACGCGGAATGATACTGCCAGGGGTTCGGCTTCAGATGAGAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

438

Amino Acids

49.41

Weight (kDa)

6.18

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 38, 91, 920
AccBSI CCGCTC 1 cut(s) 700
AccII CGCG 3 cut(s) 34, 535, 1270
AciI CCGC 6 cut(s) 34, 266, 469, 535, 700, 1270
AclWI GGATC 3 cut(s) 452, 1163, 1245
AcoI YGGCCR 3 cut(s) 536, 666, 1068
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 3 cut(s) 737, 806, 1281
AcyI GRCGYC 1 cut(s) 577
AfaI GTAC 2 cut(s) 143, 704
AfiI CCNNNNNNNGG 7 cut(s) 225, 324, 325, 475, 514, 787, 903
AflII CTTAAG 4 cut(s) 112, 332, 839, 991
AgsI TTSAA 4 cut(s) 152, 646, 811, 1052
AjnI CCWGG 5 cut(s) 16, 171, 447, 460, 1283
AjuI GAANNNNNNNTTGG 2 cut(s) 1224, 1256
AluBI AGCT 9 cut(s) 64, 121, 497, 598, 614, 649, 733, 807, 1043
AluI AGCT 9 cut(s) 64, 121, 497, 598, 614, 649, 733, 807, 1043
Alw21I GWGCWC 3 cut(s) 441, 600, 1036
Alw26I GTCTC 2 cut(s) 359, 1142
AlwI GGATC 3 cut(s) 452, 1163, 1245
AlwNI CAGNNNCTG 1 cut(s) 17
AoxI GGCC 5 cut(s) 338, 536, 666, 744, 1068
ApeKI GCWGC 3 cut(s) 730, 974, 1176
ApoI RAATTY 1 cut(s) 276
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
AspS9I GGNCC 3 cut(s) 328, 338, 745
AsuHPI GGTGA 2 cut(s) 836, 1241
AsuII TTCGAA 1 cut(s) 1105
AvaII GGWCC 1 cut(s) 328
AxyI CCTNAGG 1 cut(s) 833
BaeGI GKGCMC 2 cut(s) 41, 96
BanI GGYRCC 3 cut(s) 38, 91, 920
BanII GRGCYC 2 cut(s) 600, 609
BarI GAAGNNNNNNTAC 2 cut(s) 246, 278
BbsI GAAGAC 3 cut(s) 261, 359, 645
Bbv12I GWGCWC 3 cut(s) 441, 600, 1036
BbvI GCAGC 3 cut(s) 742, 961, 1188
BccI CCATC 8 cut(s) 50, 178, 307, 317, 911, 1060, 1085, 1253
BceAI ACGGC 2 cut(s) 371, 1055
BciT130I CCWGG 5 cut(s) 18, 173, 449, 462, 1285
BciVI GTATCC 1 cut(s) 904
BcoDI GTCTC 2 cut(s) 359, 1142
BfaI CTAG 2 cut(s) 213, 1173
BfrI CTTAAG 4 cut(s) 112, 332, 839, 991
BfuI GTATCC 1 cut(s) 904
BisI GCNGC 4 cut(s) 536, 731, 975, 1177
BlsI GCNGC 4 cut(s) 537, 732, 976, 1178
Bme1390I CCNGG 5 cut(s) 18, 173, 449, 462, 1285
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 3 cut(s) 328, 338, 745
BmiI GGNNCC 8 cut(s) 40, 93, 297, 329, 339, 407, 466, 922
BmrFI CCNGG 5 cut(s) 18, 173, 449, 462, 1285
BmsI GCATC 3 cut(s) 1141, 1201, 1237
BoxI GACNNNNGTC 3 cut(s) 313, 359, 1154
BpiI GAAGAC 3 cut(s) 261, 359, 645
BpmI CTGGAG 2 cut(s) 378, 965
Bpu10I CCTNAGC 2 cut(s) 610, 615
Bpu14I TTCGAA 1 cut(s) 1105
BpuEI CTTGAG 2 cut(s) 541, 1020
Bsa29I ATCGAT 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 1131
BsaHI GRCGYC 1 cut(s) 577
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 7 cut(s) 172, 289, 509, 798, 885, 897, 1284
BsaWI WCCGGW 1 cut(s) 636
Bsc4I CCNNNNNNNGG 7 cut(s) 225, 324, 325, 475, 514, 787, 903
Bse1I ACTGG 3 cut(s) 236, 361, 948
Bse21I CCTNAGG 1 cut(s) 833
Bse8I GATNNNNATC 1 cut(s) 1131
BseBI CCWGG 5 cut(s) 18, 173, 449, 462, 1285
BseCI ATCGAT 1 cut(s) 182
BseDI CCNNGG 7 cut(s) 172, 289, 509, 798, 885, 897, 1284
BseJI GATNNNNATC 1 cut(s) 1131
BseLI CCNNNNNNNGG 7 cut(s) 225, 324, 325, 475, 514, 787, 903
BseMII CTCAG 2 cut(s) 785, 1235
BseNI ACTGG 3 cut(s) 236, 361, 948
BseSI GKGCMC 2 cut(s) 41, 96
BseXI GCAGC 3 cut(s) 742, 961, 1188
Bsh1236I CGCG 3 cut(s) 34, 535, 1270
Bsh1285I CGRYCG 2 cut(s) 182, 637
BshFI GGCC 5 cut(s) 340, 538, 668, 746, 1070
BshNI GGYRCC 3 cut(s) 38, 91, 920
BshVI ATCGAT 1 cut(s) 182
BsiEI CGRYCG 2 cut(s) 182, 637
BsiHKAI GWGCWC 3 cut(s) 441, 600, 1036
BsiSI CCGG 1 cut(s) 637
BslFI GGGAC 5 cut(s) 189, 223, 301, 491, 774
BslI CCNNNNNNNGG 7 cut(s) 225, 324, 325, 475, 514, 787, 903
BsmAI GTCTC 2 cut(s) 359, 1142
BsmFI GGGAC 5 cut(s) 189, 223, 301, 491, 774
BsmI GAATGC 2 cut(s) 954, 1098
BsnI GGCC 5 cut(s) 340, 538, 668, 746, 1070
Bso31I GGTCTC 1 cut(s) 359
Bsp119I TTCGAA 1 cut(s) 1105
Bsp1286I GDGCHC 6 cut(s) 41, 96, 441, 600, 609, 1036
Bsp143I GATC 5 cut(s) 179, 457, 1078, 1168, 1237
Bsp19I CCATGG 1 cut(s) 798
BspACI CCGC 6 cut(s) 34, 266, 469, 535, 700, 1270
BspANI GGCC 5 cut(s) 340, 538, 668, 746, 1070
BspCNI CTCAG 2 cut(s) 784, 1234
BspDI ATCGAT 1 cut(s) 182
BspFNI CGCG 3 cut(s) 34, 535, 1270
BspLI GGNNCC 8 cut(s) 40, 93, 297, 329, 339, 407, 466, 922
BspPI GGATC 3 cut(s) 452, 1163, 1245
BspQI GCTCTTC 1 cut(s) 944
BspT104I TTCGAA 1 cut(s) 1105
BspT107I GGYRCC 3 cut(s) 38, 91, 920
BspTI CTTAAG 4 cut(s) 112, 332, 839, 991
BspTNI GGTCTC 1 cut(s) 359
BsrBI CCGCTC 1 cut(s) 700
BsrI ACTGG 3 cut(s) 236, 361, 948
BssECI CCNNGG 7 cut(s) 172, 289, 509, 798, 885, 897, 1284
BssMI GATC 5 cut(s) 179, 457, 1078, 1168, 1237
BssNI GRCGYC 1 cut(s) 577
BssT1I CCWWGG 3 cut(s) 798, 885, 897
Bst2UI CCWGG 5 cut(s) 18, 173, 449, 462, 1285
Bst4CI ACNGT 7 cut(s) 51, 146, 370, 414, 445, 870, 1031
Bst6I CTCTTC 2 cut(s) 944, 1227
BstACI GRCGYC 1 cut(s) 577
BstAFI CTTAAG 4 cut(s) 112, 332, 839, 991
BstBI TTCGAA 1 cut(s) 1105
BstC8I GCNNGC 4 cut(s) 119, 342, 499, 1045
BstDEI CTNAG 8 cut(s) 157, 312, 610, 615, 771, 833, 925, 1221
BstDSI CCRYGG 2 cut(s) 509, 798
BstFNI CGCG 3 cut(s) 34, 535, 1270
BstKTI GATC 5 cut(s) 182, 460, 1081, 1171, 1240
BstMAI GTCTC 2 cut(s) 359, 1142
BstMBI GATC 5 cut(s) 179, 457, 1078, 1168, 1237
BstMCI CGRYCG 2 cut(s) 182, 637
BstMWI GCNNNNNNNGC 3 cut(s) 392, 604, 1040
BstNI CCWGG 5 cut(s) 18, 173, 449, 462, 1285
BstPAI GACNNNNGTC 3 cut(s) 313, 359, 1154
BstSCI CCNGG 5 cut(s) 16, 171, 447, 460, 1283
BstSLI GKGCMC 2 cut(s) 41, 96
BstUI CGCG 3 cut(s) 34, 535, 1270
BstV1I GCAGC 3 cut(s) 742, 961, 1188
BstV2I GAAGAC 3 cut(s) 261, 359, 645
BstX2I RGATCY 1 cut(s) 1237
BstXI CCANNNNNNTGG 1 cut(s) 53
BstYI RGATCY 1 cut(s) 1237
Bsu15I ATCGAT 1 cut(s) 182
Bsu36I CCTNAGG 1 cut(s) 833
BsuI GTATCC 1 cut(s) 904
BsuRI GGCC 5 cut(s) 340, 538, 668, 746, 1070
BsuTUI ATCGAT 1 cut(s) 182
BtgI CCRYGG 2 cut(s) 509, 798
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 3 cut(s) 243, 441, 508
Cac8I GCNNGC 4 cut(s) 119, 342, 499, 1045
CaiI CAGNNNCTG 1 cut(s) 17
Cfr13I GGNCC 3 cut(s) 328, 338, 745
ClaI ATCGAT 1 cut(s) 182
CseI GACGC 3 cut(s) 40, 566, 1276
Csp6I GTAC 2 cut(s) 142, 703
CviAII CATG 4 cut(s) 473, 664, 799, 1226
CviQI GTAC 2 cut(s) 142, 703
DdeI CTNAG 8 cut(s) 157, 312, 610, 615, 771, 833, 925, 1221
DpnI GATC 5 cut(s) 181, 459, 1080, 1170, 1239
DpnII GATC 5 cut(s) 179, 457, 1078, 1168, 1237
EaeI YGGCCR 3 cut(s) 536, 666, 1068
Eam1104I CTCTTC 2 cut(s) 944, 1227
EarI CTCTTC 2 cut(s) 944, 1227
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 3 cut(s) 798, 885, 897
Eco24I GRGCYC 2 cut(s) 600, 609
Eco31I GGTCTC 1 cut(s) 359
Eco32I GATATC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 3 cut(s) 737, 806, 1281
Eco81I CCTNAGG 1 cut(s) 833
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 2 cut(s) 328, 338
EcoRII CCWGG 5 cut(s) 16, 171, 447, 460, 1283
EcoRV GATATC 1 cut(s) 556
EcoT14I CCWWGG 3 cut(s) 798, 885, 897
EcoT38I GRGCYC 2 cut(s) 600, 609
ErhI CCWWGG 3 cut(s) 798, 885, 897
FaeI CATG 4 cut(s) 476, 667, 802, 1229
FalI AAGNNNNNCTT 2 cut(s) 1224, 1256
FaqI GGGAC 5 cut(s) 189, 223, 301, 491, 774
FatI CATG 4 cut(s) 472, 663, 798, 1225
FauI CCCGC 2 cut(s) 27, 476
FauNDI CATATG 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 536, 731, 975, 1177
FriOI GRGCYC 2 cut(s) 600, 609
Fsp4HI GCNGC 4 cut(s) 536, 731, 975, 1177
FspBI CTAG 2 cut(s) 213, 1173
GluI GCNGC 4 cut(s) 536, 731, 975, 1177
GsuI CTGGAG 2 cut(s) 378, 965
HaeIII GGCC 5 cut(s) 340, 538, 668, 746, 1070
HapII CCGG 1 cut(s) 637
HgaI GACGC 3 cut(s) 40, 566, 1276
Hin1I GRCGYC 1 cut(s) 577
Hin1II CATG 4 cut(s) 476, 667, 802, 1229
HinfI GANTC 6 cut(s) 360, 582, 623, 769, 1187, 1219
HpaII CCGG 1 cut(s) 637
HphI GGTGA 2 cut(s) 836, 1241
Hpy166II GTNNAC 1 cut(s) 739
Hpy188III TCNNGA 3 cut(s) 364, 677, 1037
Hpy8I GTNNAC 1 cut(s) 739
Hpy99I CGWCG 2 cut(s) 141, 582
HpyCH4III ACNGT 7 cut(s) 51, 146, 370, 414, 445, 870, 1031
HpyCH4V TGCA 5 cut(s) 395, 688, 982, 1214, 1250
HpyF10VI GCNNNNNNNGC 3 cut(s) 392, 604, 1040
HpyF3I CTNAG 8 cut(s) 157, 312, 610, 615, 771, 833, 925, 1221
Hsp92I GRCGYC 1 cut(s) 577
Hsp92II CATG 4 cut(s) 476, 667, 802, 1229
Kzo9I GATC 5 cut(s) 179, 457, 1078, 1168, 1237
LguI GCTCTTC 1 cut(s) 944
LmnI GCTCC 4 cut(s) 61, 405, 436, 1095
Lsp1109I GCAGC 3 cut(s) 742, 961, 1188
LweI GCATC 3 cut(s) 1141, 1201, 1237
MaeI CTAG 2 cut(s) 213, 1173
MalI GATC 5 cut(s) 181, 459, 1080, 1170, 1239
MbiI CCGCTC 1 cut(s) 700
MboI GATC 5 cut(s) 179, 457, 1078, 1168, 1237
MboII GAAGA 9 cut(s) 266, 364, 645, 961, 990, 1094, 1130, 1193, 1244
MfeI CAATTG 1 cut(s) 562
MflI RGATCY 1 cut(s) 1237
MhlI GDGCHC 6 cut(s) 41, 96, 441, 600, 609, 1036
MluCI AATT 4 cut(s) 276, 562, 845, 911
MlyI GAGTC 4 cut(s) 369, 763, 1196, 1213
MmeI TCCRAC 4 cut(s) 70, 805, 852, 983
MseI TTAA 5 cut(s) 113, 333, 840, 992, 1020
MspCI CTTAAG 4 cut(s) 112, 332, 839, 991
MspI CCGG 1 cut(s) 637
MspR9I CCNGG 5 cut(s) 18, 173, 449, 462, 1285
MunI CAATTG 1 cut(s) 562
Mva1269I GAATGC 2 cut(s) 954, 1098
MvaI CCWGG 5 cut(s) 18, 173, 449, 462, 1285
MvnI CGCG 3 cut(s) 34, 535, 1270
MwoI GCNNNNNNNGC 3 cut(s) 392, 604, 1040
NcoI CCATGG 1 cut(s) 798
NdeI CATATG 1 cut(s) 658
NdeII GATC 5 cut(s) 179, 457, 1078, 1168, 1237
NlaIII CATG 4 cut(s) 476, 667, 802, 1229
NlaIV GGNNCC 8 cut(s) 40, 93, 297, 329, 339, 407, 466, 922
NmeAIII GCCGAG 1 cut(s) 694
NspV TTCGAA 1 cut(s) 1105
PciSI GCTCTTC 1 cut(s) 944
PctI GAATGC 2 cut(s) 954, 1098
PfeI GAWTC 2 cut(s) 582, 623
PfoI TCCNGGA 1 cut(s) 447
PkrI GCNGC 4 cut(s) 537, 732, 976, 1178
Ple19I CGATCG 1 cut(s) 182
PleI GAGTC 4 cut(s) 368, 763, 1195, 1213
PpsI GAGTC 4 cut(s) 368, 763, 1195, 1213
PpuMI RGGWCCY 1 cut(s) 328
PshAI GACNNNNGTC 3 cut(s) 313, 359, 1154
Psp124BI GAGCTC 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 5 cut(s) 16, 171, 447, 460, 1283
PspGI CCWGG 5 cut(s) 16, 171, 447, 460, 1283
PspN4I GGNNCC 8 cut(s) 40, 93, 297, 329, 339, 407, 466, 922
PspPI GGNCC 3 cut(s) 328, 338, 745
PspPPI RGGWCCY 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 1237
PvuI CGATCG 1 cut(s) 182
RsaI GTAC 2 cut(s) 143, 704
RsaNI GTAC 2 cut(s) 142, 703
SacI GAGCTC 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 944
SaqAI TTAA 5 cut(s) 113, 333, 840, 992, 1020
SatI GCNGC 4 cut(s) 536, 731, 975, 1177
Sau3AI GATC 5 cut(s) 179, 457, 1078, 1168, 1237
Sau96I GGNCC 3 cut(s) 328, 338, 745
SchI GAGTC 4 cut(s) 369, 763, 1196, 1213
ScrFI CCNGG 5 cut(s) 18, 173, 449, 462, 1285
SduI GDGCHC 6 cut(s) 41, 96, 441, 600, 609, 1036
SfaNI GCATC 3 cut(s) 1141, 1201, 1237
SfuI TTCGAA 1 cut(s) 1105
SinI GGWCC 1 cut(s) 328
SmlI CTYRAG 6 cut(s) 112, 332, 520, 839, 991, 1035
SmoI CTYRAG 6 cut(s) 112, 332, 520, 839, 991, 1035
Sse9I AATT 4 cut(s) 276, 562, 845, 911
SsiI CCGC 6 cut(s) 34, 266, 469, 535, 700, 1270
SspMI CTAG 2 cut(s) 213, 1173
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 5 cut(s) 16, 171, 447, 460, 1283
StyI CCWWGG 3 cut(s) 798, 885, 897
TaaI ACNGT 7 cut(s) 51, 146, 370, 414, 445, 870, 1031
TaqI TCGA 7 cut(s) 182, 363, 580, 591, 1001, 1105, 1138
TasI AATT 4 cut(s) 276, 562, 845, 911
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 2 cut(s) 582, 623
Tru1I TTAA 5 cut(s) 113, 333, 840, 992, 1020
Tru9I TTAA 5 cut(s) 113, 333, 840, 992, 1020
TscAI CASTG 3 cut(s) 243, 448, 508
TseI GCWGC 3 cut(s) 730, 974, 1176
TspDTI ATGAA 6 cut(s) 269, 636, 645, 807, 998, 1212
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 3 cut(s) 243, 448, 508
Vha464I CTTAAG 4 cut(s) 112, 332, 839, 991
VpaK11BI GGWCC 1 cut(s) 328
XapI RAATTY 1 cut(s) 276
XspI CTAG 2 cut(s) 213, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.