MD02G1250300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013723
Physical Location & Seq
Reverse (-)
30154480 .. 30155886
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1250300.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGCCCATGTTAGTTCCTTCAGAAGACATCCTGTTTCACAAGGAAGCTCGCAAGCACCGAGTGAGACCAATCATTAAAACTAAGTCTCGAGAAGAAGTCCTCAAGGTTGCTGCCTCGAAGAAAGCTGAAGCTGAGGCCATTGGATGTGCTGCTGCCATAGTTGCCGGGGAAGATAGGCGATTGTTGCCTCATCTTCCTACTATCAATCCTATCTTTCCTCCAGTTAGGGAGCACATTGGGCAGAAAGGTGATCCTAGTTCTAGCAGCAAGGGTAAAGATAAAGAAGAGGTTGGCAGTGTCCCTTGGAAGGATTTGAAGGTTGCCATGCGACCAAAGGACTTTGGGTATATCAACAATTGCCTGGCAGGGCGTCGATTCACTTTCGATGAGCTCGGAGAGCCTTTAGCTAAAGATGAATCGGATTGTGACCGGATGTTGAAGCTGTCTTCATATGTCATGACTGAGTATCACGACAGACTGCGAGAGGCTGAGCGGTGCAAGGTGAAACTGAAAGAGAATAAGCAGCTTGTGAATGATGCCAGGAAGACGAGCAAAGCTTTGGCTAAGGCCATTGAAGTCAGGGATCAACATTTTGAGAGTTTGAAGAGGCGGAATGGTGAGAACTTGAGACTCAAAGTACAGTTGGAGGCGACTAAGAAACAGTTGGAGACAACTTTGCTCGAGGTTTCCAAGGTTAAAGGGGAGTTGGATAGTGCCTTGGTTGAGGTTTCTGAGCTGAAGAGTAGTATCCCGACTGAGAAGGAAGCTGCTGTGAAGGAGTTCTTGGGTTCCCCGGCCTTTCTCCATGTCCTTAGACCTCGCTGTACCCGGGAAGTTCACTTTGAAAAAAGGAAATGGATGGCTGTCCTTGATCGTTATGATGATGGAAGCGTTCTTCGAAAATATCGTGAAGAAATAGATGAGCATCATCGAAAAGGTGAAACCTTCAACCTTGCCGTTTATTCTAGCAGTGCAGATGAGTCTGGTGATGAGGCTAGTGCTGATGAGCAGAGTCATCAGGGTGATGATGAGTTTGGAAATGCAGAAAATGGCGGTAGGACTCATAGTGATATGATCAGGGGTTCAACCTCAGATGAGGATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

369

Amino Acids

41.62

Weight (kDa)

6.5

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 493
AciI CCGC 3 cut(s) 493, 610, 1053
AclWI GGATC 2 cut(s) 245, 591
AcuI CTGAAG 2 cut(s) 147, 758
AcyI GRCGYC 1 cut(s) 370
AdeI CACNNNGTG 1 cut(s) 61
AfaI GTAC 2 cut(s) 639, 826
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 7 cut(s) 316, 439, 575, 604, 845, 949, 1086
AjnI CCWGG 2 cut(s) 360, 539
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
Alw21I GWGCWC 2 cut(s) 234, 393
Alw26I GTCTC 4 cut(s) 58, 90, 622, 662
AlwI GGATC 2 cut(s) 245, 591
Ama87I CYCGRG 3 cut(s) 87, 680, 828
AoxI GGCC 3 cut(s) 135, 567, 795
ApeKI GCWGC 6 cut(s) 110, 149, 152, 264, 523, 767
Asp700I GAANNNNTTC 1 cut(s) 779
AsuC2I CCSGG 4 cut(s) 166, 794, 829, 830
AsuHPI GGTGA 6 cut(s) 260, 514, 629, 950, 998, 1034
AsuII TTCGAA 1 cut(s) 898
AvaI CYCGRG 3 cut(s) 87, 680, 828
BanII GRGCYC 1 cut(s) 393
BbsI GAAGAC 3 cut(s) 30, 438, 551
Bbv12I GWGCWC 2 cut(s) 234, 393
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 6 cut(s) 97, 136, 139, 276, 535, 754
BccI CCATC 2 cut(s) 853, 878
BceAI ACGGC 1 cut(s) 941
BciT130I CCWGG 2 cut(s) 362, 541
BciVI GTATCC 1 cut(s) 758
BclI TGATCA 1 cut(s) 1074
BcnI CCSGG 4 cut(s) 166, 794, 829, 830
BcoDI GTCTC 4 cut(s) 58, 90, 622, 662
BfaI CTAG 5 cut(s) 255, 261, 966, 996, 1105
BfuI GTATCC 1 cut(s) 758
BisI GCNGC 6 cut(s) 111, 150, 153, 265, 524, 768
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 6 cut(s) 112, 151, 154, 266, 525, 769
Bme1390I CCNGG 6 cut(s) 166, 362, 541, 794, 829, 830
BmeT110I CYCGRG 3 cut(s) 87, 680, 828
BmiI GGNNCC 1 cut(s) 790
BmrFI CCNGG 6 cut(s) 166, 362, 541, 794, 829, 830
BmsI GCATC 2 cut(s) 526, 934
BpiI GAAGAC 3 cut(s) 30, 438, 551
BpmI CTGGAG 1 cut(s) 204
Bpu10I CCTNAGC 2 cut(s) 132, 564
Bpu1102I GCTNAGC 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 898
BpuEI CTTGAG 2 cut(s) 86, 646
BpuMI CCSGG 4 cut(s) 166, 794, 829, 830
BsaBI GATNNNNATC 1 cut(s) 924
BsaHI GRCGYC 1 cut(s) 370
BsaI GGTCTC 1 cut(s) 58
BsaJI CCNNGG 6 cut(s) 165, 302, 690, 717, 792, 828
BsaWI WCCGGW 1 cut(s) 429
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 221
Bse8I GATNNNNATC 1 cut(s) 924
BseBI CCWGG 2 cut(s) 362, 541
BseDI CCNNGG 6 cut(s) 165, 302, 690, 717, 792, 828
BseGI GGATG 5 cut(s) 27, 149, 438, 864, 1105
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 6 cut(s) 123, 453, 480, 723, 747, 1104
BseNI ACTGG 1 cut(s) 221
BseXI GCAGC 6 cut(s) 97, 136, 139, 276, 535, 754
BsgI GTGCAG 1 cut(s) 993
BshFI GGCC 3 cut(s) 137, 569, 797
BsiHKAI GWGCWC 2 cut(s) 234, 393
BsiHKCI CYCGRG 3 cut(s) 87, 680, 828
BsiSI CCGG 4 cut(s) 165, 430, 794, 829
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 4 cut(s) 58, 90, 622, 662
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 3 cut(s) 137, 569, 797
Bso31I GGTCTC 1 cut(s) 58
BsoBI CYCGRG 3 cut(s) 87, 680, 828
Bsp119I TTCGAA 1 cut(s) 898
Bsp1286I GDGCHC 2 cut(s) 234, 393
Bsp143I GATC 4 cut(s) 250, 583, 871, 1074
Bsp1720I GCTNAGC 1 cut(s) 489
BspACI CCGC 3 cut(s) 493, 610, 1053
BspANI GGCC 3 cut(s) 137, 569, 797
BspCNI CTCAG 6 cut(s) 124, 454, 481, 724, 748, 1103
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 1 cut(s) 790
BspPI GGATC 2 cut(s) 245, 591
BspT104I TTCGAA 1 cut(s) 898
BspTNI GGTCTC 1 cut(s) 58
BsrBI CCGCTC 1 cut(s) 493
BsrI ACTGG 1 cut(s) 221
BssECI CCNNGG 6 cut(s) 165, 302, 690, 717, 792, 828
BssMI GATC 4 cut(s) 250, 583, 871, 1074
BssNI GRCGYC 1 cut(s) 370
BssT1I CCWWGG 3 cut(s) 302, 690, 717
Bst2UI CCWGG 2 cut(s) 362, 541
Bst4CI ACNGT 2 cut(s) 642, 663
Bst6I CTCTTC 3 cut(s) 279, 599, 734
BstACI GRCGYC 1 cut(s) 370
BstBI TTCGAA 1 cut(s) 898
BstC8I GCNNGC 2 cut(s) 49, 53
BstF5I GGATG 5 cut(s) 27, 149, 438, 864, 1105
BstKTI GATC 4 cut(s) 253, 586, 874, 1077
BstMAI GTCTC 4 cut(s) 58, 90, 622, 662
BstMBI GATC 4 cut(s) 250, 583, 871, 1074
BstMWI GCNNNNNNNGC 4 cut(s) 161, 184, 238, 397
BstNI CCWGG 2 cut(s) 362, 541
BstSCI CCNGG 6 cut(s) 164, 360, 539, 792, 827, 828
BstV1I GCAGC 6 cut(s) 97, 136, 139, 276, 535, 754
BstV2I GAAGAC 3 cut(s) 30, 438, 551
BsuI GTATCC 1 cut(s) 758
BsuRI GGCC 3 cut(s) 137, 569, 797
BtsCI GGATG 5 cut(s) 27, 149, 438, 864, 1105
BtsI GCAGTG 2 cut(s) 301, 976
BtsIMutI CAGTG 2 cut(s) 301, 976
Cac8I GCNNGC 2 cut(s) 49, 53
CciI TCATGA 1 cut(s) 456
Cfr9I CCCGGG 1 cut(s) 828
CseI GACGC 1 cut(s) 359
Csp6I GTAC 2 cut(s) 638, 825
CviAII CATG 4 cut(s) 7, 325, 457, 806
CviQI GTAC 2 cut(s) 638, 825
DpnI GATC 4 cut(s) 252, 585, 873, 1076
DpnII GATC 4 cut(s) 250, 583, 871, 1074
DraIII CACNNNGTG 1 cut(s) 61
Eam1104I CTCTTC 3 cut(s) 279, 599, 734
EarI CTCTTC 3 cut(s) 279, 599, 734
EciI GGCGGA 1 cut(s) 625
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 3 cut(s) 302, 690, 717
Eco24I GRGCYC 1 cut(s) 393
Eco31I GGTCTC 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 391
Eco57I CTGAAG 2 cut(s) 147, 758
Eco88I CYCGRG 3 cut(s) 87, 680, 828
EcoICRI GAGCTC 1 cut(s) 391
EcoRII CCWGG 2 cut(s) 360, 539
EcoT14I CCWWGG 3 cut(s) 302, 690, 717
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 3 cut(s) 302, 690, 717
FaeI CATG 4 cut(s) 10, 328, 460, 809
FalI AAGNNNNNCTT 2 cut(s) 767, 799
FaqI GGGAC 1 cut(s) 284
FatI CATG 4 cut(s) 6, 324, 456, 805
FauNDI CATATG 1 cut(s) 451
FbaI TGATCA 1 cut(s) 1074
Fnu4HI GCNGC 6 cut(s) 111, 150, 153, 265, 524, 768
FokI GGATG 4 cut(s) 14, 156, 445, 871
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 6 cut(s) 111, 150, 153, 265, 524, 768
FspBI CTAG 5 cut(s) 255, 261, 966, 996, 1105
GluI GCNGC 6 cut(s) 111, 150, 153, 265, 524, 768
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 3 cut(s) 137, 569, 797
HapII CCGG 4 cut(s) 165, 430, 794, 829
HgaI GACGC 1 cut(s) 359
Hin1I GRCGYC 1 cut(s) 370
Hin1II CATG 4 cut(s) 10, 328, 460, 809
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 6 cut(s) 375, 416, 630, 980, 1012, 1060
HpaII CCGG 4 cut(s) 165, 430, 794, 829
HphI GGTGA 6 cut(s) 260, 514, 629, 950, 998, 1034
Hpy166II GTNNAC 1 cut(s) 838
Hpy188I TCNGA 5 cut(s) 22, 395, 421, 733, 1093
Hpy188III TCNNGA 6 cut(s) 87, 89, 457, 470, 751, 908
Hpy8I GTNNAC 1 cut(s) 838
Hpy99I CGWCG 1 cut(s) 375
HpyAV CCTTC 6 cut(s) 27, 301, 310, 754, 769, 955
HpyCH4III ACNGT 2 cut(s) 642, 663
HpyCH4V TGCA 3 cut(s) 498, 974, 1043
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 184, 238, 397
Hsp92I GRCGYC 1 cut(s) 370
Hsp92II CATG 4 cut(s) 10, 328, 460, 809
Ksp22I TGATCA 1 cut(s) 1074
Kzo9I GATC 4 cut(s) 250, 583, 871, 1074
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 6 cut(s) 97, 136, 139, 276, 535, 754
LweI GCATC 2 cut(s) 526, 934
MaeI CTAG 5 cut(s) 255, 261, 966, 996, 1105
MaeIII GTNAC 1 cut(s) 425
MalI GATC 4 cut(s) 252, 585, 873, 1076
MbiI CCGCTC 1 cut(s) 493
MboI GATC 4 cut(s) 250, 583, 871, 1074
MfeI CAATTG 1 cut(s) 355
MhlI GDGCHC 2 cut(s) 234, 393
MluCI AATT 1 cut(s) 355
MlyI GAGTC 4 cut(s) 624, 989, 1021, 1054
MmeI TCCRAC 3 cut(s) 624, 645, 687
MroXI GAANNNNTTC 1 cut(s) 779
MseI TTAA 2 cut(s) 75, 696
MslI CAYNNNNRTG 1 cut(s) 1020
MspI CCGG 4 cut(s) 165, 430, 794, 829
MspR9I CCNGG 6 cut(s) 166, 362, 541, 794, 829, 830
MunI CAATTG 1 cut(s) 355
MvaI CCWGG 2 cut(s) 362, 541
MwoI GCNNNNNNNGC 4 cut(s) 161, 184, 238, 397
NciI CCSGG 4 cut(s) 166, 794, 829, 830
NdeI CATATG 1 cut(s) 451
NdeII GATC 4 cut(s) 250, 583, 871, 1074
NlaIII CATG 4 cut(s) 10, 328, 460, 809
NlaIV GGNNCC 1 cut(s) 790
NmuCI GTSAC 1 cut(s) 425
NspV TTCGAA 1 cut(s) 898
PaeR7I CTCGAG 2 cut(s) 87, 680
PagI TCATGA 1 cut(s) 456
PcsI WCGNNNNNNNCGW 2 cut(s) 55, 904
PdmI GAANNNNTTC 1 cut(s) 779
PfeI GAWTC 2 cut(s) 375, 416
PkrI GCNGC 6 cut(s) 112, 151, 154, 266, 525, 769
PleI GAGTC 4 cut(s) 624, 988, 1020, 1054
PpsI GAGTC 4 cut(s) 624, 988, 1020, 1054
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 2 cut(s) 360, 539
PspGI CCWGG 2 cut(s) 360, 539
PspN4I GGNNCC 1 cut(s) 790
PspXI VCTCGAGB 1 cut(s) 680
RsaI GTAC 2 cut(s) 639, 826
RsaNI GTAC 2 cut(s) 638, 825
RseI CAYNNNNRTG 1 cut(s) 1020
SacI GAGCTC 1 cut(s) 393
SaqAI TTAA 2 cut(s) 75, 696
SatI GCNGC 6 cut(s) 111, 150, 153, 265, 524, 768
Sau3AI GATC 4 cut(s) 250, 583, 871, 1074
SchI GAGTC 4 cut(s) 624, 989, 1021, 1054
ScrFI CCNGG 6 cut(s) 166, 362, 541, 794, 829, 830
SduI GDGCHC 2 cut(s) 234, 393
SfaNI GCATC 2 cut(s) 526, 934
Sfr274I CTCGAG 2 cut(s) 87, 680
SfuI TTCGAA 1 cut(s) 898
SlaI CTCGAG 2 cut(s) 87, 680
SmaI CCCGGG 1 cut(s) 830
SmiMI CAYNNNNRTG 1 cut(s) 1020
SmlI CTYRAG 4 cut(s) 87, 101, 625, 680
SmoI CTYRAG 4 cut(s) 87, 101, 625, 680
Sse9I AATT 1 cut(s) 355
SsiI CCGC 3 cut(s) 493, 610, 1053
SspMI CTAG 5 cut(s) 255, 261, 966, 996, 1105
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 6 cut(s) 164, 360, 539, 792, 827, 828
StyI CCWWGG 3 cut(s) 302, 690, 717
TaaI ACNGT 2 cut(s) 642, 663
TaqI TCGA 7 cut(s) 88, 116, 373, 384, 681, 898, 931
TasI AATT 1 cut(s) 355
TatI WGTACW 1 cut(s) 637
TfiI GAWTC 2 cut(s) 375, 416
Tru1I TTAA 2 cut(s) 75, 696
Tru9I TTAA 2 cut(s) 75, 696
TscAI CASTG 2 cut(s) 301, 976
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 6 cut(s) 110, 149, 152, 264, 523, 767
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 2 cut(s) 429, 438
TspMI CCCGGG 1 cut(s) 828
TspRI CASTG 2 cut(s) 301, 976
XhoI CTCGAG 2 cut(s) 87, 680
XmaI CCCGGG 1 cut(s) 828
XmnI GAANNNNTTC 1 cut(s) 779
XspI CTAG 5 cut(s) 255, 261, 966, 996, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.