MD03G1186300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013723
Physical Location & Seq
Forward (+)
25396987 .. 25398724
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1186300.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGGAGTGTGGGTTGTGTGAGCCTTCCTTCCATCAGTGGCGTTACTGTTACAAGATGCGCCCAGCAAAATCATGCACTGGTTATGCCGAGTGTGCATGTCGGAGTGAGAGAGAGCGTATTGTGTATGGTAAGAAAAAGGCATACTACACATGGAAAAACCGTTGGTGCTTTCTGTATAATGATTGGGAGTATGATAAGGGTGTCACGCCTGAGCGACGTGTGCTTACTCACTTCCAGACTGTAGGTTGTAACGTATCAACCGTTCGTACTATTTGCTATTTGTTGTGGTCTTTTCTTGCTTCTAACACTTTGCTTCATGTAGTGACGCGGGGCACCATCCAACTGTTTGGGCAGGAGCTATCTGACATAGAGAAGGTGTTGAGGGTGCCCAAAGAGGATAGACACTTAAGCAAGCTACGACCCTTATTTCGTCGGTACGGTTTCCAACCCTTAGTTTCCGAGAGCCAGGGACGATCGAGTAAGTTGTATCCTTCATTGGAGAAGGTAAGCAAGAAAACAGGGACTAGCACCCATAAAAGGAAAGCACCAGTGTTAGTTCCTTCGGAAGACATCCTACCGCATAAGAAAATTCATAAGTTCCGAGGGGAACCATCCGTTAGACCTAAGTCCCAAGATGGGGTCCTTAAGGGGCCTGCCTTTAGGAAGACTGGAGTCGAGGCCGTTGATAATGCTGCTGCCGTAGTTGCAGGAGAAGGGAGCCGACTGTTGCCTCATCCTCTTACTATGGAGCACACTGTCCAGGAAAGTGATCCTGGTTCCCGCCATGAGGGGAAAGGCAAGGAAAGAGCTGGCAGTGTCCCGTGGAAGGACTTGAGGGTTGCCACGCGGCCAAAGGATTTTGGGGATATCAACAATTGCTTGGCAGGGCGTCGATTCGCCTTCGATGAGCTCGGAGAGCCCTTAGCTAAGGATGAATCGGATTGCGACCGGATGTTGAAGCTGTCTTCATATGTCATGGCCGAGTATCACGACAGACTGCAAGAGGTTGAGCGGTACAAGGCAAAACTGAAGGAGAATAAGCAGCTTGTGGACGAGGCCCGAAGGAATAAGGGACTTTTGACTCAGGCTCTCCAACTGAAGGACGAAACCATGGAGAGCTTGAAAAGGCGAAATGGTGAGAACCTAAGGCTTAAGAAATTGTTTGAGGCAACTAAAAAACAGTTGGAGGTGGCTACCTTGGAAGTATCCAAGGTTAGGGGAGAATTGGATGGTGCCTTAGTTGAGATTTCTGAACTGGAGAAGAGCATTCCAACTGAAAGGGAGGCTGCTGTGCAAGAATACTTAAGTTCTTCGACCTTTCATCTTGCTATTAAACCCTACTGTGCTCAAGAAGCTCGCTTTGAAAAAAGGAAATGGATGGCCGTCCTTGATCGTTATGATGATGGGAGCATTCTTCGAAAATACCACGAAGATATAGATGAGCATCATCGAAAGGGCGAGACATTTGTCCTTGCTGTTGATCCTAGCAGCGAAGATGAGTCTGATAATGAAGGTAGTGCTGATGCACAGACTCAGCATGGTGAAGAGGATCTTGGGGATGCAGAGGATGATGGTAGGACGCGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

530

Amino Acids

60.59

Weight (kDa)

8.61

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 332, 385, 1232
AccBSI CCGCTC 1 cut(s) 1012
AccII CGCG 3 cut(s) 328, 847, 1582
AciI CCGC 6 cut(s) 328, 578, 781, 847, 1012, 1582
AclWI GGATC 3 cut(s) 764, 1475, 1557
AcoI YGGCCR 3 cut(s) 848, 978, 1380
AcsI RAATTY 1 cut(s) 588
AcuI CTGAAG 2 cut(s) 1049, 1118
AcyI GRCGYC 1 cut(s) 889
AfaI GTAC 3 cut(s) 268, 437, 1016
AfiI CCNNNNNNNGG 7 cut(s) 537, 636, 637, 787, 826, 1099, 1215
AflII CTTAAG 4 cut(s) 406, 644, 1151, 1303
AflIII ACRYGT 1 cut(s) 217
AgsI TTSAA 3 cut(s) 958, 1123, 1364
AjiI CACGTC 1 cut(s) 218
AjnI CCWGG 3 cut(s) 465, 759, 772
AjuI GAANNNNNNNTTGG 2 cut(s) 1536, 1568
AluBI AGCT 9 cut(s) 358, 415, 809, 910, 926, 961, 1045, 1119, 1355
AluI AGCT 9 cut(s) 358, 415, 809, 910, 926, 961, 1045, 1119, 1355
Alw21I GWGCWC 3 cut(s) 753, 912, 1348
Alw26I GTCTC 1 cut(s) 1454
AlwI GGATC 3 cut(s) 764, 1475, 1557
AoxI GGCC 6 cut(s) 650, 678, 848, 978, 1056, 1380
ApeKI GCWGC 5 cut(s) 692, 695, 1042, 1286, 1488
ApoI RAATTY 1 cut(s) 588
ArsI GACNNNNNNTTYG 2 cut(s) 411, 443
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 3 cut(s) 640, 650, 1057
AsuHPI GGTGA 2 cut(s) 1148, 1553
AsuII TTCGAA 1 cut(s) 1417
AvaII GGWCC 1 cut(s) 640
AxyI CCTNAGG 1 cut(s) 1145
BaeGI GKGCMC 2 cut(s) 335, 390
BanI GGYRCC 3 cut(s) 332, 385, 1232
BanII GRGCYC 2 cut(s) 912, 921
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BbsI GAAGAC 3 cut(s) 573, 671, 957
Bbv12I GWGCWC 3 cut(s) 753, 912, 1348
BbvI GCAGC 5 cut(s) 679, 682, 1054, 1273, 1500
BccI CCATC 8 cut(s) 39, 344, 619, 629, 1223, 1372, 1397, 1565
BceAI ACGGC 3 cut(s) 665, 683, 1367
BciT130I CCWGG 3 cut(s) 467, 761, 774
BciVI GTATCC 2 cut(s) 498, 1216
BcoDI GTCTC 1 cut(s) 1454
BfaI CTAG 2 cut(s) 525, 1485
BfmI CTRYAG 1 cut(s) 240
BfrI CTTAAG 4 cut(s) 406, 644, 1151, 1303
BfuI GTATCC 2 cut(s) 498, 1216
BisI GCNGC 6 cut(s) 693, 696, 848, 1043, 1287, 1489
BlsI GCNGC 6 cut(s) 694, 697, 849, 1044, 1288, 1490
Bme1390I CCNGG 3 cut(s) 467, 761, 774
Bme18I GGWCC 1 cut(s) 640
BmgBI CACGTC 1 cut(s) 218
BmgT120I GGNCC 3 cut(s) 640, 650, 1057
BmiI GGNNCC 8 cut(s) 334, 387, 609, 641, 651, 719, 778, 1234
BmrFI CCNGG 3 cut(s) 467, 761, 774
BmsI GCATC 4 cut(s) 45, 1453, 1513, 1549
BoxI GACNNNNGTC 3 cut(s) 625, 671, 1466
BpiI GAAGAC 3 cut(s) 573, 671, 957
BpmI CTGGAG 2 cut(s) 690, 1277
Bpu10I CCTNAGC 3 cut(s) 210, 922, 927
Bpu14I TTCGAA 1 cut(s) 1417
BpuEI CTTGAG 2 cut(s) 853, 1332
BsaBI GATNNNNATC 1 cut(s) 1443
BsaHI GRCGYC 1 cut(s) 889
BsaJI CCNNGG 6 cut(s) 466, 601, 821, 1110, 1197, 1209
BsaWI WCCGGW 1 cut(s) 948
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 7 cut(s) 537, 636, 637, 787, 826, 1099, 1215
Bse1I ACTGG 4 cut(s) 82, 548, 673, 1260
Bse21I CCTNAGG 1 cut(s) 1145
Bse8I GATNNNNATC 1 cut(s) 1443
BseBI CCWGG 3 cut(s) 467, 761, 774
BseDI CCNNGG 6 cut(s) 466, 601, 821, 1110, 1197, 1209
BseJI GATNNNNATC 1 cut(s) 1443
BseLI CCNNNNNNNGG 7 cut(s) 537, 636, 637, 787, 826, 1099, 1215
BseMII CTCAG 3 cut(s) 201, 1097, 1547
BseNI ACTGG 4 cut(s) 82, 548, 673, 1260
BseSI GKGCMC 2 cut(s) 335, 390
BseXI GCAGC 5 cut(s) 679, 682, 1054, 1273, 1500
BseYI CCCAGC 1 cut(s) 61
Bsh1236I CGCG 3 cut(s) 328, 847, 1582
Bsh1285I CGRYCG 2 cut(s) 476, 949
BshFI GGCC 6 cut(s) 652, 680, 850, 980, 1058, 1382
BshNI GGYRCC 3 cut(s) 332, 385, 1232
BsiEI CGRYCG 2 cut(s) 476, 949
BsiHKAI GWGCWC 3 cut(s) 753, 912, 1348
BsiSI CCGG 1 cut(s) 949
BslFI GGGAC 5 cut(s) 483, 535, 613, 803, 1086
BslI CCNNNNNNNGG 7 cut(s) 537, 636, 637, 787, 826, 1099, 1215
BsmAI GTCTC 1 cut(s) 1454
BsmFI GGGAC 5 cut(s) 483, 535, 613, 803, 1086
BsmI GAATGC 2 cut(s) 1266, 1410
BsnI GGCC 6 cut(s) 652, 680, 850, 980, 1058, 1382
Bsp119I TTCGAA 1 cut(s) 1417
Bsp1286I GDGCHC 6 cut(s) 335, 390, 753, 912, 921, 1348
Bsp143I GATC 5 cut(s) 473, 769, 1390, 1480, 1549
Bsp19I CCATGG 1 cut(s) 1110
BspACI CCGC 6 cut(s) 328, 578, 781, 847, 1012, 1582
BspANI GGCC 6 cut(s) 652, 680, 850, 980, 1058, 1382
BspCNI CTCAG 3 cut(s) 202, 1096, 1546
BspFNI CGCG 3 cut(s) 328, 847, 1582
BspLI GGNNCC 8 cut(s) 334, 387, 609, 641, 651, 719, 778, 1234
BspPI GGATC 3 cut(s) 764, 1475, 1557
BspQI GCTCTTC 1 cut(s) 1256
BspT104I TTCGAA 1 cut(s) 1417
BspT107I GGYRCC 3 cut(s) 332, 385, 1232
BspTI CTTAAG 4 cut(s) 406, 644, 1151, 1303
BsrBI CCGCTC 1 cut(s) 1012
BsrI ACTGG 4 cut(s) 82, 548, 673, 1260
BssECI CCNNGG 6 cut(s) 466, 601, 821, 1110, 1197, 1209
BssMI GATC 5 cut(s) 473, 769, 1390, 1480, 1549
BssNI GRCGYC 1 cut(s) 889
BssT1I CCWWGG 3 cut(s) 1110, 1197, 1209
Bst2UI CCWGG 3 cut(s) 467, 761, 774
Bst6I CTCTTC 2 cut(s) 1256, 1539
BstACI GRCGYC 1 cut(s) 889
BstAFI CTTAAG 4 cut(s) 406, 644, 1151, 1303
BstBI TTCGAA 1 cut(s) 1417
BstC8I GCNNGC 4 cut(s) 413, 654, 811, 1357
BstDEI CTNAG 9 cut(s) 210, 451, 624, 922, 927, 1083, 1145, 1237, 1533
BstDSI CCRYGG 2 cut(s) 821, 1110
BstFNI CGCG 3 cut(s) 328, 847, 1582
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 5 cut(s) 476, 772, 1393, 1483, 1552
BstMAI GTCTC 1 cut(s) 1454
BstMBI GATC 5 cut(s) 473, 769, 1390, 1480, 1549
BstMCI CGRYCG 2 cut(s) 476, 949
BstMWI GCNNNNNNNGC 5 cut(s) 92, 220, 704, 916, 1352
BstNI CCWGG 3 cut(s) 467, 761, 774
BstNSI RCATGY 1 cut(s) 99
BstPAI GACNNNNGTC 3 cut(s) 625, 671, 1466
BstSCI CCNGG 3 cut(s) 465, 759, 772
BstSFI CTRYAG 1 cut(s) 240
BstSLI GKGCMC 2 cut(s) 335, 390
BstUI CGCG 3 cut(s) 328, 847, 1582
BstV1I GCAGC 5 cut(s) 679, 682, 1054, 1273, 1500
BstV2I GAAGAC 3 cut(s) 573, 671, 957
BstX2I RGATCY 1 cut(s) 1549
BstXI CCANNNNNNTGG 1 cut(s) 347
BstYI RGATCY 1 cut(s) 1549
Bsu36I CCTNAGG 1 cut(s) 1145
BsuI GTATCC 2 cut(s) 498, 1216
BsuRI GGCC 6 cut(s) 652, 680, 850, 980, 1058, 1382
BtgI CCRYGG 2 cut(s) 821, 1110
BtrI CACGTC 1 cut(s) 218
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 5 cut(s) 41, 75, 555, 753, 820
Cac8I GCNNGC 4 cut(s) 413, 654, 811, 1357
CfoI GCGC 1 cut(s) 60
Cfr13I GGNCC 3 cut(s) 640, 650, 1057
CseI GACGC 2 cut(s) 334, 878
Csp6I GTAC 3 cut(s) 267, 436, 1015
CviAII CATG 8 cut(s) 72, 96, 150, 317, 785, 976, 1111, 1538
CviQI GTAC 3 cut(s) 267, 436, 1015
DdeI CTNAG 9 cut(s) 210, 451, 624, 922, 927, 1083, 1145, 1237, 1533
DpnI GATC 5 cut(s) 475, 771, 1392, 1482, 1551
DpnII GATC 5 cut(s) 473, 769, 1390, 1480, 1549
EaeI YGGCCR 3 cut(s) 848, 978, 1380
Eam1104I CTCTTC 2 cut(s) 1256, 1539
EarI CTCTTC 2 cut(s) 1256, 1539
Ecl136II GAGCTC 1 cut(s) 910
Eco130I CCWWGG 3 cut(s) 1110, 1197, 1209
Eco24I GRGCYC 2 cut(s) 912, 921
Eco32I GATATC 1 cut(s) 868
Eco47I GGWCC 1 cut(s) 640
Eco53kI GAGCTC 1 cut(s) 910
Eco57I CTGAAG 2 cut(s) 1049, 1118
Eco81I CCTNAGG 1 cut(s) 1145
EcoICRI GAGCTC 1 cut(s) 910
EcoO109I RGGNCCY 2 cut(s) 640, 650
EcoRII CCWGG 3 cut(s) 465, 759, 772
EcoRV GATATC 1 cut(s) 868
EcoT14I CCWWGG 3 cut(s) 1110, 1197, 1209
EcoT38I GRGCYC 2 cut(s) 912, 921
ErhI CCWWGG 3 cut(s) 1110, 1197, 1209
FaeI CATG 8 cut(s) 75, 99, 153, 320, 788, 979, 1114, 1541
FalI AAGNNNNNCTT 2 cut(s) 1536, 1568
FaqI GGGAC 5 cut(s) 483, 535, 613, 803, 1086
FatI CATG 8 cut(s) 71, 95, 149, 316, 784, 975, 1110, 1537
FauI CCCGC 2 cut(s) 321, 788
FauNDI CATATG 1 cut(s) 970
Fnu4HI GCNGC 6 cut(s) 693, 696, 848, 1043, 1287, 1489
FriOI GRGCYC 2 cut(s) 912, 921
Fsp4HI GCNGC 6 cut(s) 693, 696, 848, 1043, 1287, 1489
FspBI CTAG 2 cut(s) 525, 1485
GlaI GCGC 1 cut(s) 59
GluI GCNGC 6 cut(s) 693, 696, 848, 1043, 1287, 1489
GsaI CCCAGC 1 cut(s) 65
GsuI CTGGAG 2 cut(s) 690, 1277
HaeIII GGCC 6 cut(s) 652, 680, 850, 980, 1058, 1382
HapII CCGG 1 cut(s) 949
HgaI GACGC 2 cut(s) 334, 878
HhaI GCGC 1 cut(s) 60
Hin1I GRCGYC 1 cut(s) 889
Hin1II CATG 8 cut(s) 75, 99, 153, 320, 788, 979, 1114, 1541
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HinfI GANTC 6 cut(s) 672, 894, 935, 1081, 1499, 1531
HpaII CCGG 1 cut(s) 949
HphI GGTGA 2 cut(s) 1148, 1553
Hpy166II GTNNAC 1 cut(s) 1051
Hpy188I TCNGA 9 cut(s) 102, 364, 460, 565, 602, 914, 940, 1252, 1504
Hpy188III TCNNGA 3 cut(s) 235, 989, 1349
Hpy8I GTNNAC 1 cut(s) 1051
Hpy99I CGWCG 3 cut(s) 219, 435, 894
HpyCH4IV ACGT 2 cut(s) 217, 252
HpyCH4V TGCA 7 cut(s) 75, 95, 707, 1000, 1294, 1526, 1562
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 220, 704, 916, 1352
HpyF3I CTNAG 9 cut(s) 210, 451, 624, 922, 927, 1083, 1145, 1237, 1533
HpySE526I ACGT 2 cut(s) 217, 252
Hsp92I GRCGYC 1 cut(s) 889
Hsp92II CATG 8 cut(s) 75, 99, 153, 320, 788, 979, 1114, 1541
HspAI GCGC 1 cut(s) 58
Kzo9I GATC 5 cut(s) 473, 769, 1390, 1480, 1549
LguI GCTCTTC 1 cut(s) 1256
LmnI GCTCC 4 cut(s) 355, 717, 748, 1407
Lsp1109I GCAGC 5 cut(s) 679, 682, 1054, 1273, 1500
LweI GCATC 4 cut(s) 45, 1453, 1513, 1549
MaeI CTAG 2 cut(s) 525, 1485
MaeII ACGT 2 cut(s) 217, 252
MaeIII GTNAC 5 cut(s) 41, 47, 202, 248, 322
MalI GATC 5 cut(s) 475, 771, 1392, 1482, 1551
MbiI CCGCTC 1 cut(s) 1012
MboI GATC 5 cut(s) 473, 769, 1390, 1480, 1549
MboII GAAGA 9 cut(s) 578, 676, 957, 1273, 1302, 1406, 1442, 1505, 1556
MfeI CAATTG 1 cut(s) 874
MflI RGATCY 1 cut(s) 1549
MhlI GDGCHC 6 cut(s) 335, 390, 753, 912, 921, 1348
MluCI AATT 4 cut(s) 588, 874, 1157, 1223
MlyI GAGTC 4 cut(s) 681, 1075, 1508, 1525
MmeI TCCRAC 6 cut(s) 80, 364, 469, 1117, 1164, 1295
MseI TTAA 5 cut(s) 407, 645, 1152, 1304, 1332
MspCI CTTAAG 4 cut(s) 406, 644, 1151, 1303
MspI CCGG 1 cut(s) 949
MspR9I CCNGG 3 cut(s) 467, 761, 774
MunI CAATTG 1 cut(s) 874
Mva1269I GAATGC 2 cut(s) 1266, 1410
MvaI CCWGG 3 cut(s) 467, 761, 774
MvnI CGCG 3 cut(s) 328, 847, 1582
MwoI GCNNNNNNNGC 5 cut(s) 92, 220, 704, 916, 1352
NcoI CCATGG 1 cut(s) 1110
NdeI CATATG 1 cut(s) 970
NdeII GATC 5 cut(s) 473, 769, 1390, 1480, 1549
NlaIII CATG 8 cut(s) 75, 99, 153, 320, 788, 979, 1114, 1541
NlaIV GGNNCC 8 cut(s) 334, 387, 609, 641, 651, 719, 778, 1234
NmeAIII GCCGAG 2 cut(s) 112, 1006
NmuCI GTSAC 2 cut(s) 202, 322
NspI RCATGY 1 cut(s) 99
NspV TTCGAA 1 cut(s) 1417
PciSI GCTCTTC 1 cut(s) 1256
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 2 cut(s) 1266, 1410
PfeI GAWTC 2 cut(s) 894, 935
PfoI TCCNGGA 1 cut(s) 759
PkrI GCNGC 6 cut(s) 694, 697, 849, 1044, 1288, 1490
Ple19I CGATCG 1 cut(s) 476
PleI GAGTC 4 cut(s) 680, 1075, 1507, 1525
PpsI GAGTC 4 cut(s) 680, 1075, 1507, 1525
PpuMI RGGWCCY 1 cut(s) 640
PshAI GACNNNNGTC 3 cut(s) 625, 671, 1466
Psp124BI GAGCTC 1 cut(s) 912
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 3 cut(s) 465, 759, 772
PspFI CCCAGC 1 cut(s) 61
PspGI CCWGG 3 cut(s) 465, 759, 772
PspN4I GGNNCC 8 cut(s) 334, 387, 609, 641, 651, 719, 778, 1234
PspPI GGNCC 3 cut(s) 640, 650, 1057
PspPPI RGGWCCY 1 cut(s) 640
PsrI GAACNNNNNNTAC 2 cut(s) 246, 278
PsuI RGATCY 1 cut(s) 1549
PvuI CGATCG 1 cut(s) 476
RsaI GTAC 3 cut(s) 268, 437, 1016
RsaNI GTAC 3 cut(s) 267, 436, 1015
SacI GAGCTC 1 cut(s) 912
SapI GCTCTTC 1 cut(s) 1256
SaqAI TTAA 5 cut(s) 407, 645, 1152, 1304, 1332
SatI GCNGC 6 cut(s) 693, 696, 848, 1043, 1287, 1489
Sau3AI GATC 5 cut(s) 473, 769, 1390, 1480, 1549
Sau96I GGNCC 3 cut(s) 640, 650, 1057
SchI GAGTC 4 cut(s) 681, 1075, 1508, 1525
ScrFI CCNGG 3 cut(s) 467, 761, 774
SduI GDGCHC 6 cut(s) 335, 390, 753, 912, 921, 1348
SfaNI GCATC 4 cut(s) 45, 1453, 1513, 1549
SfcI CTRYAG 1 cut(s) 240
SfuI TTCGAA 1 cut(s) 1417
SinI GGWCC 1 cut(s) 640
SmlI CTYRAG 6 cut(s) 406, 644, 832, 1151, 1303, 1347
SmoI CTYRAG 6 cut(s) 406, 644, 832, 1151, 1303, 1347
Sse9I AATT 4 cut(s) 588, 874, 1157, 1223
SsiI CCGC 6 cut(s) 328, 578, 781, 847, 1012, 1582
SspMI CTAG 2 cut(s) 525, 1485
SstI GAGCTC 1 cut(s) 912
StyD4I CCNGG 3 cut(s) 465, 759, 772
StyI CCWWGG 3 cut(s) 1110, 1197, 1209
TaiI ACGT 2 cut(s) 220, 255
TaqI TCGA 7 cut(s) 476, 675, 892, 903, 1313, 1417, 1450
TasI AATT 4 cut(s) 588, 874, 1157, 1223
TauI GCSGC 1 cut(s) 850
TfiI GAWTC 2 cut(s) 894, 935
Tru1I TTAA 5 cut(s) 407, 645, 1152, 1304, 1332
Tru9I TTAA 5 cut(s) 407, 645, 1152, 1304, 1332
TscAI CASTG 5 cut(s) 41, 82, 555, 760, 820
TseFI GTSAC 2 cut(s) 202, 322
TseI GCWGC 5 cut(s) 692, 695, 1042, 1286, 1488
Tsp45I GTSAC 2 cut(s) 202, 322
TspDTI ATGAA 7 cut(s) 305, 483, 581, 948, 957, 1310, 1524
TspGWI ACGGA 1 cut(s) 604
TspRI CASTG 5 cut(s) 41, 82, 555, 760, 820
Vha464I CTTAAG 4 cut(s) 406, 644, 1151, 1303
VpaK11BI GGWCC 1 cut(s) 640
XapI RAATTY 1 cut(s) 588
XceI RCATGY 1 cut(s) 99
XspI CTAG 2 cut(s) 525, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.