pycom16g24020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013723
Physical Location & Seq
Reverse (-)
25264466 .. 25265818
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g24020.4

Sequence Viewer

Length: 855 bp
ATGTCAGAGTCTGGAAGTCCTAGTGATGAGGGCTCCCCTAGCTCTAGCTCTAGGTTTGAGCTTGCAATGTTGGAGTCTTTAGGGCCTTTGTTGGAGTCTTGTACCAAAGAAACGTTGGATGATTTTTGCAATTGTCAAACCTTAGCCAATATTGGTTCTTCCTCCTTCATGTCAATGGGTGAATGGGTTGTTTTTGACGCCATACCCATATTTCGCTTTGAGTTCACAGCGGACCTTTTAGAGAAAAACTTGTTAGATAGCGAAAGGCAACTTGAGGCCCTAAGGCAGTCATGTAGTATCCCCCGCAGTGTAGGAATGCGTTTGTGGCGCTACTGCTATAAGATGCGCCCGGTGAAAGCATGCCCTGGGTATGCCAAGTGTGCATGTCGGAGTGAGAGAGAGCATATTGTCTTTGTGACGCGAGGCAACATCAAATTGTCCGGACAGGAGCTGGCTGATGTAGAGAAAGTGTTAAAAGTGCCTAAGGAGGATAGACATTTGGGCAAGCTATGGCCTTTGTTTCAAAAGTACGGTTTCCAGCCCCTAGTGTTGAAGTGCCAAAGACGAGCAATGGAGAAAGTGAGCAAGAAAGGGGGGACTAGCACCAAGAAAGGGAAAGCATCCATGTTAGTTCCCGTAGATGACATTTTGTTTCACAAAAGAGCTCGTAAACACCGAGTGAGGCCAACCCCTAGACCTAAGTCACAAAAGGGGGTCTTCAAGATTACTGCCTCAAAGAAAGCTGAAGCTGAGGCGATCGGGTATGCTGCTGCCATAGTTGCAGGGGATGAGAGACGACTGTTGCATGGCACATGGAATATGTGCAGCGCATGGCACTCAGATTTAAGTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

31.91

Weight (kDa)

9.39

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 421
AccIII TCCGGA 1 cut(s) 440
AciI CCGC 2 cut(s) 230, 304
AclI AACGTT 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 1 cut(s) 198
AdeI CACNNNGTG 1 cut(s) 679
AfaI GTAC 2 cut(s) 103, 530
AfiI CCNNNNNNNGG 1 cut(s) 612
AgsI TTSAA 3 cut(s) 524, 553, 721
AjnI CCWGG 1 cut(s) 364
AluBI AGCT 8 cut(s) 42, 48, 61, 451, 508, 665, 743, 749
AluI AGCT 8 cut(s) 42, 48, 61, 451, 508, 665, 743, 749
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 1 cut(s) 787
AlwNI CAGNNNCTG 2 cut(s) 11, 451
Aor13HI TCCGGA 1 cut(s) 440
AoxI GGCC 4 cut(s) 83, 276, 512, 683
ApeKI GCWGC 3 cut(s) 767, 770, 825
AspLEI GCGC 3 cut(s) 330, 348, 830
AspS9I GGNCC 3 cut(s) 83, 232, 277
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 191, 364
AvaII GGWCC 1 cut(s) 232
AxyI CCTNAGG 2 cut(s) 281, 483
BanII GRGCYC 2 cut(s) 35, 667
BbsI GAAGAC 1 cut(s) 709
Bbv12I GWGCWC 1 cut(s) 667
BbvCI CCTCAGC 1 cut(s) 750
BbvI GCAGC 3 cut(s) 754, 757, 837
BciT130I CCWGG 1 cut(s) 366
BciVI GTATCC 1 cut(s) 308
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 1 cut(s) 787
BfaI CTAG 7 cut(s) 21, 39, 45, 51, 545, 600, 693
BfoI RGCGCY 1 cut(s) 331
BfuI GTATCC 1 cut(s) 308
BisI GCNGC 3 cut(s) 768, 771, 826
BlsI GCNGC 3 cut(s) 769, 772, 827
Bme1390I CCNGG 2 cut(s) 350, 366
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 3 cut(s) 83, 232, 277
BmiI GGNNCC 1 cut(s) 34
BmrFI CCNGG 2 cut(s) 350, 366
BmsI GCATC 2 cut(s) 333, 629
BoxI GACNNNNGTC 1 cut(s) 700
BpiI GAAGAC 1 cut(s) 709
Bpu10I CCTNAGC 2 cut(s) 142, 750
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 350
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 364, 365
BsaWI WCCGGW 1 cut(s) 440
Bsc4I CCNNNNNNNGG 1 cut(s) 612
Bse21I CCTNAGG 2 cut(s) 281, 483
Bse3DI GCAATG 2 cut(s) 72, 576
BseAI TCCGGA 1 cut(s) 440
BseBI CCWGG 1 cut(s) 366
BseDI CCNNGG 2 cut(s) 364, 365
BseGI GGATG 3 cut(s) 124, 620, 793
BseLI CCNNNNNNNGG 1 cut(s) 612
BseMI GCAATG 2 cut(s) 72, 576
BseMII CTCAG 2 cut(s) 741, 852
BseXI GCAGC 3 cut(s) 754, 757, 837
BsgI GTGCAG 1 cut(s) 844
Bsh1236I CGCG 1 cut(s) 421
Bsh1285I CGRYCG 1 cut(s) 759
BshFI GGCC 4 cut(s) 85, 278, 514, 685
BsiEI CGRYCG 1 cut(s) 759
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 2 cut(s) 350, 441
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 1 cut(s) 612
BsmAI GTCTC 1 cut(s) 787
BsmBI CGTCTC 1 cut(s) 787
BsmFI GGGAC 1 cut(s) 610
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 4 cut(s) 85, 278, 514, 685
Bsp1286I GDGCHC 2 cut(s) 35, 667
Bsp13I TCCGGA 1 cut(s) 440
Bsp143I GATC 1 cut(s) 756
BspACI CCGC 2 cut(s) 230, 304
BspANI GGCC 4 cut(s) 85, 278, 514, 685
BspCNI CTCAG 2 cut(s) 742, 851
BspEI TCCGGA 1 cut(s) 440
BspFNI CGCG 1 cut(s) 421
BspLI GGNNCC 1 cut(s) 34
BsrDI GCAATG 2 cut(s) 72, 576
BssECI CCNNGG 2 cut(s) 364, 365
BssMI GATC 1 cut(s) 756
BssNI GRCGYC 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 2 cut(s) 533, 801
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 4 cut(s) 63, 361, 453, 506
BstDEI CTNAG 6 cut(s) 142, 281, 483, 699, 750, 838
BstF5I GGATG 3 cut(s) 124, 620, 793
BstFNI CGCG 1 cut(s) 421
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 3 cut(s) 330, 348, 830
BstKTI GATC 1 cut(s) 759
BstMAI GTCTC 1 cut(s) 787
BstMBI GATC 1 cut(s) 756
BstMCI CGRYCG 1 cut(s) 759
BstMWI GCNNNNNNNGC 4 cut(s) 39, 325, 380, 779
BstNI CCWGG 1 cut(s) 366
BstNSI RCATGY 2 cut(s) 363, 387
BstPAI GACNNNNGTC 1 cut(s) 700
BstSCI CCNGG 2 cut(s) 348, 364
BstUI CGCG 1 cut(s) 421
BstV1I GCAGC 3 cut(s) 754, 757, 837
BstV2I GAAGAC 1 cut(s) 709
Bsu36I CCTNAGG 2 cut(s) 281, 483
BsuI GTATCC 1 cut(s) 308
BsuRI GGCC 4 cut(s) 85, 278, 514, 685
BtsCI GGATG 3 cut(s) 124, 620, 793
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 1 cut(s) 313
Cac8I GCNNGC 4 cut(s) 63, 361, 453, 506
CaiI CAGNNNCTG 2 cut(s) 11, 451
CfoI GCGC 3 cut(s) 330, 348, 830
Cfr13I GGNCC 3 cut(s) 83, 232, 277
CseI GACGC 2 cut(s) 206, 427
Csp6I GTAC 2 cut(s) 102, 529
CviAII CATG 8 cut(s) 169, 291, 360, 384, 625, 806, 813, 831
CviQI GTAC 2 cut(s) 102, 529
DdeI CTNAG 6 cut(s) 142, 281, 483, 699, 750, 838
DpnI GATC 1 cut(s) 758
DpnII GATC 1 cut(s) 756
DraIII CACNNNGTG 1 cut(s) 679
Ecl136II GAGCTC 1 cut(s) 665
Eco24I GRGCYC 2 cut(s) 35, 667
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 665
Eco57I CTGAAG 1 cut(s) 765
Eco81I CCTNAGG 2 cut(s) 281, 483
EcoICRI GAGCTC 1 cut(s) 665
EcoO109I RGGNCCY 2 cut(s) 83, 277
EcoRII CCWGG 1 cut(s) 364
EcoT38I GRGCYC 2 cut(s) 35, 667
Esp3I CGTCTC 1 cut(s) 787
FaeI CATG 8 cut(s) 172, 294, 363, 387, 628, 809, 816, 834
FalI AAGNNNNNCTT 2 cut(s) 701, 733
FaqI GGGAC 1 cut(s) 610
FatI CATG 8 cut(s) 168, 290, 359, 383, 624, 805, 812, 830
FauI CCCGC 1 cut(s) 311
Fnu4HI GCNGC 3 cut(s) 768, 771, 826
FokI GGATG 3 cut(s) 131, 607, 800
FriOI GRGCYC 2 cut(s) 35, 667
Fsp4HI GCNGC 3 cut(s) 768, 771, 826
FspBI CTAG 7 cut(s) 21, 39, 45, 51, 545, 600, 693
GlaI GCGC 3 cut(s) 329, 347, 829
GluI GCNGC 3 cut(s) 768, 771, 826
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 4 cut(s) 85, 278, 514, 685
HapII CCGG 2 cut(s) 350, 441
HgaI GACGC 2 cut(s) 206, 427
HhaI GCGC 3 cut(s) 330, 348, 830
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 8 cut(s) 172, 294, 363, 387, 628, 809, 816, 834
Hin6I GCGC 3 cut(s) 328, 346, 828
HinP1I GCGC 3 cut(s) 328, 346, 828
HinfI GANTC 3 cut(s) 8, 74, 95
HpaII CCGG 2 cut(s) 350, 441
HphI GGTGA 2 cut(s) 191, 364
Hpy166II GTNNAC 2 cut(s) 225, 671
Hpy188I TCNGA 3 cut(s) 7, 390, 841
Hpy188III TCNNGA 3 cut(s) 12, 441, 721
Hpy8I GTNNAC 2 cut(s) 225, 671
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 2 cut(s) 533, 801
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 6 cut(s) 65, 129, 383, 782, 805, 825
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 325, 380, 779
HpyF3I CTNAG 6 cut(s) 142, 281, 483, 699, 750, 838
HpySE526I ACGT 1 cut(s) 113
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 8 cut(s) 172, 294, 363, 387, 628, 809, 816, 834
HspAI GCGC 3 cut(s) 328, 346, 828
Kpn2I TCCGGA 1 cut(s) 440
Kzo9I GATC 1 cut(s) 756
LmnI GCTCC 2 cut(s) 38, 448
LpnPI CCDG 8 cut(s) 351, 363, 378, 431, 437, 454, 551, 768
Lsp1109I GCAGC 3 cut(s) 754, 757, 837
LweI GCATC 2 cut(s) 333, 629
MaeI CTAG 7 cut(s) 21, 39, 45, 51, 545, 600, 693
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 2 cut(s) 415, 702
MalI GATC 1 cut(s) 758
MboI GATC 1 cut(s) 756
MboII GAAGA 2 cut(s) 150, 709
MfeI CAATTG 1 cut(s) 130
MhlI GDGCHC 2 cut(s) 35, 667
MluCI AATT 2 cut(s) 130, 434
MlyI GAGTC 3 cut(s) 17, 83, 104
MmeI TCCRAC 4 cut(s) 51, 72, 96, 368
MnlI CCTC 8 cut(s) 22, 172, 268, 416, 481, 675, 742, 745
MroI TCCGGA 1 cut(s) 440
MseI TTAA 2 cut(s) 473, 845
MslI CAYNNNNRTG 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 2 cut(s) 350, 441
MspR9I CCNGG 2 cut(s) 350, 366
MunI CAATTG 1 cut(s) 130
Mva1269I GAATGC 1 cut(s) 321
MvaI CCWGG 1 cut(s) 366
MvnI CGCG 1 cut(s) 421
MwoI GCNNNNNNNGC 4 cut(s) 39, 325, 380, 779
NciI CCSGG 1 cut(s) 350
NdeII GATC 1 cut(s) 756
NlaIII CATG 8 cut(s) 172, 294, 363, 387, 628, 809, 816, 834
NlaIV GGNNCC 1 cut(s) 34
NmuCI GTSAC 2 cut(s) 415, 702
NspI RCATGY 2 cut(s) 363, 387
PaeI GCATGC 1 cut(s) 363
PasI CCCWGGG 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 673
PctI GAATGC 1 cut(s) 321
PkrI GCNGC 3 cut(s) 769, 772, 827
Ple19I CGATCG 1 cut(s) 759
PleI GAGTC 3 cut(s) 16, 82, 103
PpsI GAGTC 3 cut(s) 16, 82, 103
PshAI GACNNNNGTC 1 cut(s) 700
Psp124BI GAGCTC 1 cut(s) 667
Psp1406I AACGTT 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 364
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 1 cut(s) 34
PspPI GGNCC 3 cut(s) 83, 232, 277
PstNI CAGNNNCTG 2 cut(s) 11, 451
PvuI CGATCG 1 cut(s) 759
RsaI GTAC 2 cut(s) 103, 530
RsaNI GTAC 2 cut(s) 102, 529
RseI CAYNNNNRTG 1 cut(s) 173
SacI GAGCTC 1 cut(s) 667
SaqAI TTAA 2 cut(s) 473, 845
SatI GCNGC 3 cut(s) 768, 771, 826
Sau3AI GATC 1 cut(s) 756
Sau96I GGNCC 3 cut(s) 83, 232, 277
SchI GAGTC 3 cut(s) 17, 83, 104
ScrFI CCNGG 2 cut(s) 350, 366
SduI GDGCHC 2 cut(s) 35, 667
SfaNI GCATC 2 cut(s) 333, 629
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 173
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
SphI GCATGC 1 cut(s) 363
Sse9I AATT 2 cut(s) 130, 434
SsiI CCGC 2 cut(s) 230, 304
SspI AATATT 1 cut(s) 151
SspMI CTAG 7 cut(s) 21, 39, 45, 51, 545, 600, 693
SstI GAGCTC 1 cut(s) 667
StyD4I CCNGG 2 cut(s) 348, 364
TaaI ACNGT 2 cut(s) 533, 801
TaiI ACGT 1 cut(s) 116
TasI AATT 2 cut(s) 130, 434
Tru1I TTAA 2 cut(s) 473, 845
Tru9I TTAA 2 cut(s) 473, 845
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 2 cut(s) 415, 702
TseI GCWGC 3 cut(s) 767, 770, 825
Tsp45I GTSAC 2 cut(s) 415, 702
TspDTI ATGAA 1 cut(s) 157
TspRI CASTG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 232
XceI RCATGY 2 cut(s) 363, 387
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XspI CTAG 7 cut(s) 21, 39, 45, 51, 545, 600, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.