MD09G1023200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013723
Physical Location & Seq
Forward (+)
1382131 .. 1382929
799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1023200.v1.1.491

Sequence Viewer

Length: 327 bp
ATGCTGACTGCCGTAGTTGCAGGAGAAGGGAGCCGACTGTTGCCTCATCCTCTTACTATGGAGCACACTGTCCAGGAAAGTGATCCTGGTTCCCGCCATGAGGGGAAAGGCAAGGAAAGAGCTGGCAGTGTCCCGTGGAAGGACTTGAGGGTTGCCACGCGGCCAAAGGATTTTGGGGATATCAACAATTGCTTGGCAGGGCGTCGATTCGCCTTCGATGAGCTCGGAGAGCCCTTAGCTAAGGATGAATCGGATTGCGACCGGATGTTGAAGCTGTCTTCATATGTGAGTCATGGCCGAGTATCACGACAGACTGCAAGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

109

Amino Acids

11.89

Weight (kDa)

7.85

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 160
AciI CCGC 2 cut(s) 94, 160
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 2 cut(s) 161, 295
AcyI GRCGYC 1 cut(s) 202
AfiI CCNNNNNNNGG 2 cut(s) 100, 139
AgsI TTSAA 1 cut(s) 271
AjnI CCWGG 2 cut(s) 72, 85
AluBI AGCT 4 cut(s) 122, 223, 239, 274
AluI AGCT 4 cut(s) 122, 223, 239, 274
Alw21I GWGCWC 2 cut(s) 66, 225
AlwI GGATC 1 cut(s) 77
AoxI GGCC 2 cut(s) 161, 295
BanII GRGCYC 2 cut(s) 225, 234
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 2 cut(s) 66, 225
BciT130I CCWGG 2 cut(s) 74, 87
BisI GCNGC 1 cut(s) 161
BlsI GCNGC 1 cut(s) 162
Bme1390I CCNGG 2 cut(s) 74, 87
BmiI GGNNCC 2 cut(s) 32, 91
BmrFI CCNGG 2 cut(s) 74, 87
BpiI GAAGAC 1 cut(s) 270
Bpu10I CCTNAGC 2 cut(s) 235, 240
BpuEI CTTGAG 1 cut(s) 166
BsaHI GRCGYC 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 134
BsaWI WCCGGW 1 cut(s) 261
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 139
BseBI CCWGG 2 cut(s) 74, 87
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 3 cut(s) 46, 250, 270
BseLI CCNNNNNNNGG 2 cut(s) 100, 139
Bsh1236I CGCG 1 cut(s) 160
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 2 cut(s) 163, 297
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 2 cut(s) 66, 225
BsiSI CCGG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 100, 139
BsmFI GGGAC 1 cut(s) 116
BsnI GGCC 2 cut(s) 163, 297
Bsp1286I GDGCHC 3 cut(s) 66, 225, 234
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 2 cut(s) 94, 160
BspANI GGCC 2 cut(s) 163, 297
BspFNI CGCG 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 32, 91
BspPI GGATC 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 1 cut(s) 82
BssNI GRCGYC 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 74, 87
Bst4CI ACNGT 2 cut(s) 39, 70
BstACI GRCGYC 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 2 cut(s) 235, 240
BstDSI CCRYGG 1 cut(s) 134
BstF5I GGATG 3 cut(s) 46, 250, 270
BstFNI CGCG 1 cut(s) 160
BstKTI GATC 1 cut(s) 85
BstMBI GATC 1 cut(s) 82
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 2 cut(s) 17, 229
BstNI CCWGG 2 cut(s) 74, 87
BstSCI CCNGG 2 cut(s) 72, 85
BstUI CGCG 1 cut(s) 160
BstV2I GAAGAC 1 cut(s) 270
BsuRI GGCC 2 cut(s) 163, 297
BtgI CCRYGG 1 cut(s) 134
BtsCI GGATG 3 cut(s) 46, 250, 270
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 2 cut(s) 66, 133
Cac8I GCNNGC 1 cut(s) 124
CseI GACGC 1 cut(s) 191
CviAII CATG 2 cut(s) 98, 293
CviJI RGCY 8 cut(s) 33, 122, 163, 223, 232, 239, 274, 297
CviKI_1 RGCY 8 cut(s) 33, 122, 163, 223, 232, 239, 274, 297
DdeI CTNAG 2 cut(s) 235, 240
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
EaeI YGGCCR 2 cut(s) 161, 295
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 2 cut(s) 225, 234
Eco32I GATATC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 223
EcoRII CCWGG 2 cut(s) 72, 85
EcoRV GATATC 1 cut(s) 181
EcoT38I GRGCYC 2 cut(s) 225, 234
FaeI CATG 2 cut(s) 101, 296
FaiI YATR 5 cut(s) 59, 99, 283, 285, 294
FaqI GGGAC 1 cut(s) 116
FatI CATG 2 cut(s) 97, 292
FauI CCCGC 1 cut(s) 101
FauNDI CATATG 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 161
FokI GGATG 3 cut(s) 33, 257, 277
FriOI GRGCYC 2 cut(s) 225, 234
Fsp4HI GCNGC 1 cut(s) 161
GluI GCNGC 1 cut(s) 161
HaeIII GGCC 2 cut(s) 163, 297
HapII CCGG 1 cut(s) 262
HgaI GACGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 202
Hin1II CATG 2 cut(s) 101, 296
HinfI GANTC 3 cut(s) 207, 248, 289
HpaII CCGG 1 cut(s) 262
Hpy188I TCNGA 2 cut(s) 227, 253
Hpy188III TCNNGA 1 cut(s) 306
Hpy99I CGWCG 1 cut(s) 207
HpyAV CCTTC 3 cut(s) 20, 133, 223
HpyCH4III ACNGT 2 cut(s) 39, 70
HpyCH4V TGCA 2 cut(s) 20, 317
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 229
HpyF3I CTNAG 2 cut(s) 235, 240
Hsp92I GRCGYC 1 cut(s) 202
Hsp92II CATG 2 cut(s) 101, 296
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 30, 61
LpnPI CCDG 8 cut(s) 6, 59, 72, 86, 99, 108, 183, 275
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 1 cut(s) 270
MfeI CAATTG 1 cut(s) 187
MhlI GDGCHC 3 cut(s) 66, 225, 234
MluCI AATT 1 cut(s) 187
MlyI GAGTC 1 cut(s) 298
MnlI CCTC 5 cut(s) 54, 60, 94, 141, 314
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 2 cut(s) 74, 87
MunI CAATTG 1 cut(s) 187
MvaI CCWGG 2 cut(s) 74, 87
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 2 cut(s) 17, 229
NdeI CATATG 1 cut(s) 283
NdeII GATC 1 cut(s) 82
NlaIII CATG 2 cut(s) 101, 296
NlaIV GGNNCC 2 cut(s) 32, 91
NmeAIII GCCGAG 1 cut(s) 323
PfeI GAWTC 2 cut(s) 207, 248
PfoI TCCNGGA 1 cut(s) 72
PkrI GCNGC 1 cut(s) 162
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 72, 85
PspGI CCWGG 2 cut(s) 72, 85
PspN4I GGNNCC 2 cut(s) 32, 91
SacI GAGCTC 1 cut(s) 225
SatI GCNGC 1 cut(s) 161
Sau3AI GATC 1 cut(s) 82
SchI GAGTC 1 cut(s) 298
ScrFI CCNGG 2 cut(s) 74, 87
SduI GDGCHC 3 cut(s) 66, 225, 234
SetI ASST 5 cut(s) 124, 225, 241, 276, 325
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 1 cut(s) 187
SsiI CCGC 2 cut(s) 94, 160
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 2 cut(s) 72, 85
TaaI ACNGT 2 cut(s) 39, 70
TaqI TCGA 2 cut(s) 205, 216
TasI AATT 1 cut(s) 187
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 2 cut(s) 207, 248
TscAI CASTG 2 cut(s) 73, 133
TspDTI ATGAA 2 cut(s) 261, 270
TspRI CASTG 2 cut(s) 73, 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.