MD00G1027900.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013733
Physical Location & Seq
Forward (+)
4590395 .. 4593117
2723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1027900.v1.1.491

Sequence Viewer

Length: 891 bp
ATGTATGCAGATTCGAGTTGGTGTCTTGACGAGCTGGTTAAGATCATGGAGTGCAGAGACAAACTGGGGCGAGATGTTTTTCCAATATTCTATCATGTTGATCCTTCACATGTCAGGAAGCAAAATGGAGATTTTGCCGAAGCATTTCAGAAGCACGAAAAGGACATCCATGAAGAAAAAGATGGCAAGACACGTGAAAAGGTAAAGCAGTGGAGATGGGCTCTTACAGAAGCTGCAAATTTGTCTGGCTGCCATCTTAAAATCACTGACAATAGGCACGAAGCAACGGTTATAAGAGAAATTATTGACAAGGTTATTACAAACTGGCTTTCGACCATAAACATATTACCAGTGGCCAAGCACCAAGTTGGAATCGATTCTCGGATTAAAGAGATTATCACTTATCTTTCAAGTGATGGATCAGATGATGTTCTCATGGTTGGAATTTGGGGGATGGGTGGATTAGGTAAAACAACAGCTGCGAAAGCCATTTTTAACAAAATTCATCATAAGTTCAAGTTCAAATGTTTCCTTGCCGACGTTCGCGACAATACAAGTAAACATGGTCTGGCTTACTTGCAAGAAAAACTTATTTTTGACGTCTTGAAAGAGAAGTCTGAAATAAGCAGTGTTGATAAAGGTATCAGTCTGATAGAAGATCAATTTTCACATAGAAAGGTACTTGTCATCATAGACAACATAGATGAAGTGGAACAACTAAATGCAATAGCTGGAAATCACAAATGGTTTGGTCCTGGAAGTAGAATTATCATAACGACACGAGATGAACATTTACTAAAGCAAGGGAAAGTGAACAAGACATATCCGGCCCAGGAATTGAATAAAGAAGAAGCTCTAAAGCTCTTGAGTTGGCATGCCTTTGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

297

Amino Acids

33.86

Weight (kDa)

7.24

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 109 2.3e-24 TIR domain
NB-ARC PF00931 127 - 294 2.7e-18 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AatII GACGTC 1 cut(s) 603
AccII CGCG 1 cut(s) 546
AclWI GGATC 2 cut(s) 95, 427
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 3 cut(s) 238, 444, 501
AcvI CACGTG 1 cut(s) 194
AcyI GRCGYC 1 cut(s) 600
AfaI GTAC 1 cut(s) 681
AflIII ACRYGT 2 cut(s) 109, 191
AgsI TTSAA 5 cut(s) 411, 517, 523, 607, 841
AjnI CCWGG 2 cut(s) 754, 831
AluBI AGCT 6 cut(s) 34, 233, 479, 731, 854, 862
AluI AGCT 6 cut(s) 34, 233, 479, 731, 854, 862
Alw26I GTCTC 1 cut(s) 51
AlwI GGATC 2 cut(s) 95, 427
AlwNI CAGNNNCTG 1 cut(s) 233
AoxI GGCC 2 cut(s) 354, 828
ApeKI GCWGC 3 cut(s) 233, 249, 479
ApoI RAATTY 3 cut(s) 238, 444, 501
Asp700I GAANNNNTTC 2 cut(s) 144, 376
AspS9I GGNCC 2 cut(s) 752, 829
AvaII GGWCC 1 cut(s) 752
BalI TGGCCA 1 cut(s) 356
BanII GRGCYC 1 cut(s) 223
BauI CACGAG 1 cut(s) 780
BbrPI CACGTG 1 cut(s) 194
BbvI GCAGC 3 cut(s) 220, 236, 466
BccI CCATC 5 cut(s) 176, 210, 261, 410, 448
BciT130I CCWGG 2 cut(s) 756, 833
BcoDI GTCTC 1 cut(s) 51
BisI GCNGC 3 cut(s) 234, 250, 480
BlsI GCNGC 3 cut(s) 235, 251, 481
Bme1390I CCNGG 2 cut(s) 756, 833
Bme18I GGWCC 1 cut(s) 752
BmgT120I GGNCC 2 cut(s) 752, 829
BmrFI CCNGG 2 cut(s) 756, 833
BmrI ACTGGG 1 cut(s) 74
BmuI ACTGGG 1 cut(s) 74
BplI GAGNNNNNCTC 2 cut(s) 205, 237
BpuEI CTTGAG 1 cut(s) 886
Bsa29I ATCGAT 1 cut(s) 375
BsaAI YACGTR 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 600
BsaJI CCNNGG 1 cut(s) 831
Bse1I ACTGG 3 cut(s) 69, 329, 350
BseBI CCWGG 2 cut(s) 756, 833
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 1 cut(s) 831
BseGI GGATG 2 cut(s) 165, 459
BseNI ACTGG 3 cut(s) 69, 329, 350
BseXI GCAGC 3 cut(s) 220, 236, 466
BsgI GTGCAG 1 cut(s) 73
Bsh1236I CGCG 1 cut(s) 546
BshFI GGCC 2 cut(s) 356, 830
BshVI ATCGAT 1 cut(s) 375
BsiSI CCGG 1 cut(s) 827
BsmAI GTCTC 1 cut(s) 51
BsnI GGCC 2 cut(s) 356, 830
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 4 cut(s) 42, 100, 419, 658
Bsp68I TCGCGA 1 cut(s) 546
BspANI GGCC 2 cut(s) 356, 830
BspDI ATCGAT 1 cut(s) 375
BspFNI CGCG 1 cut(s) 546
BspPI GGATC 2 cut(s) 95, 427
BsrI ACTGG 3 cut(s) 69, 329, 350
BssECI CCNNGG 1 cut(s) 831
BssMI GATC 4 cut(s) 42, 100, 419, 658
BssNI GRCGYC 1 cut(s) 600
BssSI CACGAG 1 cut(s) 780
Bst2BI CACGAG 1 cut(s) 780
Bst2UI CCWGG 2 cut(s) 756, 833
Bst4CI ACNGT 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 876
BstF5I GGATG 2 cut(s) 165, 459
BstFNI CGCG 1 cut(s) 546
BstKTI GATC 4 cut(s) 45, 103, 422, 661
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 4 cut(s) 42, 100, 419, 658
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNI CCWGG 2 cut(s) 756, 833
BstNSI RCATGY 2 cut(s) 113, 878
BstSCI CCNGG 2 cut(s) 754, 831
BstUI CGCG 1 cut(s) 546
BstV1I GCAGC 3 cut(s) 220, 236, 466
Bsu15I ATCGAT 1 cut(s) 375
BsuRI GGCC 2 cut(s) 356, 830
BsuTUI ATCGAT 1 cut(s) 375
BtsCI GGATG 2 cut(s) 165, 459
BtsI GCAGTG 2 cut(s) 215, 634
BtsIMutI CAGTG 4 cut(s) 215, 264, 357, 634
BtuMI TCGCGA 1 cut(s) 546
Cac8I GCNNGC 1 cut(s) 876
CaiI CAGNNNCTG 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 752, 829
ClaI ATCGAT 1 cut(s) 375
Csp6I GTAC 1 cut(s) 680
CviAII CATG 7 cut(s) 46, 95, 110, 170, 436, 563, 875
CviQI GTAC 1 cut(s) 680
DpnI GATC 4 cut(s) 44, 102, 421, 660
DpnII GATC 4 cut(s) 42, 100, 419, 658
EaeI YGGCCR 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 752
Eco72I CACGTG 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 754, 831
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 7 cut(s) 49, 98, 113, 173, 439, 566, 878
FalI AAGNNNNNCTT 2 cut(s) 573, 605
FatI CATG 7 cut(s) 45, 94, 109, 169, 435, 562, 874
Fnu4HI GCNGC 3 cut(s) 234, 250, 480
FokI GGATG 2 cut(s) 152, 466
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 3 cut(s) 234, 250, 480
GluI GCNGC 3 cut(s) 234, 250, 480
HaeIII GGCC 2 cut(s) 356, 830
HapII CCGG 1 cut(s) 827
Hin1I GRCGYC 1 cut(s) 600
Hin1II CATG 7 cut(s) 49, 98, 113, 173, 439, 566, 878
HinfI GANTC 3 cut(s) 11, 372, 377
HpaII CCGG 1 cut(s) 827
Hpy166II GTNNAC 2 cut(s) 560, 814
Hpy188I TCNGA 5 cut(s) 150, 384, 424, 619, 651
Hpy188III TCNNGA 5 cut(s) 26, 115, 545, 604, 865
Hpy8I GTNNAC 2 cut(s) 560, 814
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 1 cut(s) 114
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 3 cut(s) 193, 540, 600
HpyCH4V TGCA 5 cut(s) 8, 54, 236, 580, 725
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpySE526I ACGT 3 cut(s) 193, 540, 600
Hsp92I GRCGYC 1 cut(s) 600
Hsp92II CATG 7 cut(s) 49, 98, 113, 173, 439, 566, 878
Kzo9I GATC 4 cut(s) 42, 100, 419, 658
Lsp1109I GCAGC 3 cut(s) 220, 236, 466
MaeII ACGT 3 cut(s) 193, 540, 600
MalI GATC 4 cut(s) 44, 102, 421, 660
MboI GATC 4 cut(s) 42, 100, 419, 658
MboII GAAGA 3 cut(s) 185, 668, 860
MhlI GDGCHC 1 cut(s) 223
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 8 cut(s) 238, 300, 444, 501, 662, 765, 836, 886
MluNI TGGCCA 1 cut(s) 356
MmeI TCCRAC 2 cut(s) 349, 421
Mox20I TGGCCA 1 cut(s) 356
MroXI GAANNNNTTC 2 cut(s) 144, 376
MscI TGGCCA 1 cut(s) 356
MseI TTAA 4 cut(s) 39, 258, 387, 495
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 1 cut(s) 827
MspR9I CCNGG 2 cut(s) 756, 833
MvaI CCWGG 2 cut(s) 756, 833
MvnI CGCG 1 cut(s) 546
MwoI GCNNNNNNNGC 1 cut(s) 485
NdeII GATC 4 cut(s) 42, 100, 419, 658
NlaIII CATG 7 cut(s) 49, 98, 113, 173, 439, 566, 878
NruI TCGCGA 1 cut(s) 546
NspI RCATGY 2 cut(s) 113, 878
PaeI GCATGC 1 cut(s) 878
PciI ACATGT 1 cut(s) 109
PdmI GAANNNNTTC 2 cut(s) 144, 376
PfeI GAWTC 3 cut(s) 11, 372, 377
PfoI TCCNGGA 1 cut(s) 754
PkrI GCNGC 3 cut(s) 235, 251, 481
PmaCI CACGTG 1 cut(s) 194
PmlI CACGTG 1 cut(s) 194
Ppu21I YACGTR 1 cut(s) 194
PscI ACATGT 1 cut(s) 109
PsiI TTATAA 1 cut(s) 293
Psp6I CCWGG 2 cut(s) 754, 831
PspCI CACGTG 1 cut(s) 194
PspGI CCWGG 2 cut(s) 754, 831
PspPI GGNCC 2 cut(s) 752, 829
PstNI CAGNNNCTG 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 479
RruI TCGCGA 1 cut(s) 546
RsaI GTAC 1 cut(s) 681
RsaNI GTAC 1 cut(s) 680
SaqAI TTAA 4 cut(s) 39, 258, 387, 495
SatI GCNGC 3 cut(s) 234, 250, 480
Sau3AI GATC 4 cut(s) 42, 100, 419, 658
Sau96I GGNCC 2 cut(s) 752, 829
ScrFI CCNGG 2 cut(s) 756, 833
SduI GDGCHC 1 cut(s) 223
SinI GGWCC 1 cut(s) 752
SmlI CTYRAG 1 cut(s) 865
SmoI CTYRAG 1 cut(s) 865
SphI GCATGC 1 cut(s) 878
Sse9I AATT 8 cut(s) 238, 300, 444, 501, 662, 765, 836, 886
SspI AATATT 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 754, 831
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 3 cut(s) 196, 543, 603
TaqI TCGA 3 cut(s) 14, 332, 375
TasI AATT 8 cut(s) 238, 300, 444, 501, 662, 765, 836, 886
TfiI GAWTC 3 cut(s) 11, 372, 377
Tru1I TTAA 4 cut(s) 39, 258, 387, 495
Tru9I TTAA 4 cut(s) 39, 258, 387, 495
TscAI CASTG 4 cut(s) 215, 271, 357, 634
TseI GCWGC 3 cut(s) 233, 249, 479
TspDTI ATGAA 4 cut(s) 186, 494, 720, 801
TspRI CASTG 4 cut(s) 215, 271, 357, 634
VpaK11BI GGWCC 1 cut(s) 752
XapI RAATTY 3 cut(s) 238, 444, 501
XceI RCATGY 2 cut(s) 113, 878
XmnI GAANNNNTTC 2 cut(s) 144, 376
ZraI GACGTC 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.