MD02G1028400.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013733
Physical Location & Seq
Forward (+)
2178701 .. 2182725
4025 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1028400.v1.1.491

Sequence Viewer

Length: 2508 bp
ATGACAGCCCACGAAGCCTCCTCTTCGTCCTCCTCCACTTCCAAACTCTGGAAGTACGACGTATTCTTGAGCTTCAGGGGTGAAGACACGCGCTATGGCTTCACGAGCCACCTCCACGCGGCATTAACAGCCAGAGGGTACCAGGTCTTTATTGATGAGGACGATCTACCAAGAGGGGAAGAAATAAAAGAGAAACTGTTCCGTGCAATCGAAGAGTCGAGGATCTCTGTCATTATCTTCTCAAAGATGTATGGGGATTCGAGTTGGTGTCTTGACGAGCTGGTGAAGATCATGGAGTGTAGAGACAAACTGGGGCGACATGTTTTACCAATATTCTATCACGTTGATCCTTCGCATGTCAGGAAGCAGGACGGAGATTTAGCCGAAGCATTTCAGAAGCACGAAAAGGACATCCGTGAAGAAAAAGATGACAAGAAACGTGAAGCTAAACAAGAAAGGGTAAATCAGTGGAGAGAGGCTCTCACAAAAGCTGCAAATTTGTCTGGCCACCATCTTCAAAGCGCTAATAATAGGAAGAGGCGGAGAGAGGCTCATACAGAAGCTACAAATTTGTCTGACCACCAAACAACTGGCAACGGGCGCGAAGCAGAGTTCATTAAAAAAATTATTAACGAGAATATTTGTGAATGGCTCACTAGCACTAACGAATTACGTGTGGCCAAGCACATAGTTGGAATCGATTCTCGCGTTCAAGATATTATCACTTATCTTTCAAGTGGTGGATCAAATGATGTTCTCATGGTTGGAATTTGGGGGATGGGTGGATTGGTCAAAACAACAGTTGCCAAAGCCATTTATAACCAAATTCATCCTAAGTTTGAATTCAAAAGTTTCCTTGCCGGCATTCGCGATGCTACAAGTAAACATGATCTGGTTGATTTGCAAAAAAAACTTATTTTTGACATCTTGAAAAAGAAGCCTGAAATAAGCTGTGTTGACGAAGGTATCGACCTGATAAAACAACAATTTCGACATAGAAAGATACTTGTCATCGTGGACAACATAGATAAAGAGGAACAACTGAATGCAATAGTTGGAAGTCATGATTGGTTTGGTCCTGGAAGTAGAATTATCATAACCACACGAGTTGAACGTCTACTGCAAGACCGAATCAGTAATCTGCAAGACCGAATCAGTAATTTACCTCACGAAATTCTTGTCCTTATAGTGTCACTCTTGCCGCTAAAGGAAGCCGCAGCCACTAGTATCCTTTCTAGGCCATGGCGCCATGTCTGGGCATTTACTACGACTCTCAGTTTTGACGCTGTTAACTTTGAAGTAGGTGATACTTTGCACCGGTTCTTGGATCTTGAAGAACAAGTGAGAGACCGGGAAAGCCGCCTCTACGTGAATTGGGTCGATCACGTTCTGGACCAACATAGAGGCCAATGCATTGACCGCTTCCGGGTTTGCTTTTTTCTGCAGCGCGACTTTGCAGGTTCCATTGACAAATGGATCCAATTTGCAATGGAAAAGAGGGTTCAAATACTTGAGCTGGAGCTTTTGACAGACTCAATTGTTTTCCTTGATGATAACTACACATTTCCCCACAAACTACTATTGGGTCCGGAAGAGCAAAAGGGCCTGTTTTCTAACCTTCCAAGCCTACACCCTGGTGGATACAGTATTGCGCCTTTCGAGTCCCTCAGAGTCGTACGCTTTCAACATGTTGGTGTGACCGGTGAAACTCTTGAGTTCTTGCTGTCCAACTGTCCAGTTCTCGAACGTTTGTCGCTGAGTTTTGCCAAAGATTTAGTCAAGCTAAGAGTTACCGGCACCTCCATGGCATTGAAATATTTAGAGGTGAAGTATTGCCTTGAACTCAGCATCATTGAGATATCTGGTGCAAACATTGTCTCCTTTATTTATTGTGGACAGTTAATAGATTTGCGATTGAATAATGTGCCCCTGCTGGTTGAGGTATCCATTTACAAGTGGGGCACTTTTGCTGAATTCGTAAGGCTTGCGTTAAGTCAACTCTCATGCTGTCTTTCTCATTTAGAGACCCTGAAGCTGGATATCAGGGGAGCGGTCTACAATCAGAATCATGCGTTTCCCGCGTTATCAAATCTCAAGCATTTGGAATTATTAGTGGATGCCGACTACTCTCTGTCTCTTGGTCGCTTAGCTTCTTTCATGGAGGCAGCTCCTTACTTGCTCAATCTTGTTTTGGGGTTGGGTTTCAGTACGTCCAATGAGGACATGGAAATACCAGAGAAAGTTTCCAAACAACCCCATCACTTCCTCAAGCTAGTAGAAATAGCAGGGTATCGGGGCCACGAGTGCTGCGTAGAGCATGTCAGATACTTGGTGGAGAATGCTGTTGCACTGGAGAAGATTATTATAAATCCTGTTCGGTTTTGGTGTCGGCCGACGGGAATTGAGAGCAATGCCCTACTGGAGAATGAGGAAGAGAATGCAAGAGATCATGCTATCCAACACCTCAAGAAAAAAGTACCTTCTAGTGTAGAATTTGTGTGCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

836

Amino Acids

95.52

Weight (kDa)

6.28

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 179 4.6e-49 TIR domain
TIR_2 PF13676 21 - 122 7.5e-18 TIR domain
NB-ARC PF00931 235 - 373 1.2e-10 NB-ARC domain
LRR_At1g61320_AtMIF1 PF23622 463 - 832 9.7e-40 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 490 - 606 2.2e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 819, 2366
Acc36I ACCTGC 1 cut(s) 1448
Acc65I GGTACC 1 cut(s) 138
AccB1I GGYRCC 3 cut(s) 138, 1245, 1796
AccB7I CCANNNNNTGG 1 cut(s) 48
AccBSI CCGCTC 1 cut(s) 2051
AccI GTMKAC 2 cut(s) 1117, 2055
AccII CGCG 7 cut(s) 91, 119, 603, 708, 870, 1449, 2081
AccIII TCCGGA 1 cut(s) 1588
AciI CCGC 8 cut(s) 119, 541, 1202, 1215, 1360, 1420, 2051, 2079
AclI AACGTT 1 cut(s) 1747
AclWI GGATC 6 cut(s) 230, 341, 751, 1335, 1471, 1484
AcoI YGGCCR 3 cut(s) 505, 678, 2390
AcsI RAATTY 8 cut(s) 496, 568, 768, 825, 842, 1173, 1973, 2492
AcuI CTGAAG 2 cut(s) 58, 2051
AcyI GRCGYC 1 cut(s) 1246
AfaI GTAC 5 cut(s) 56, 140, 1677, 2209, 2478
AfeI AGCGCT 1 cut(s) 523
AfiI CCNNNNNNNGG 6 cut(s) 48, 118, 1255, 1324, 1426, 2035
AflIII ACRYGT 3 cut(s) 319, 673, 1687
AgeI ACCGGT 2 cut(s) 1317, 1700
AhlI ACTAGT 1 cut(s) 1223
AjnI CCWGG 3 cut(s) 141, 1078, 1633
AjuI GAANNNNNNNTTGG 2 cut(s) 1051, 1083
AleI CACNNNNGTG 1 cut(s) 1635
Alw26I GTCTC 5 cut(s) 297, 1341, 1882, 2018, 2139
AlwI GGATC 6 cut(s) 230, 341, 751, 1335, 1471, 1484
Aor13HI TCCGGA 1 cut(s) 1588
Aor51HI AGCGCT 1 cut(s) 523
AoxI GGCC 7 cut(s) 505, 678, 1238, 1405, 1603, 2296, 2390
ApeKI GCWGC 5 cut(s) 491, 1217, 1444, 2165, 2307
ApoI RAATTY 8 cut(s) 496, 568, 768, 825, 842, 1173, 1973, 2492
ArsI GACNNNNNNTTYG 4 cut(s) 1164, 1196, 1761, 1793
AsiGI ACCGGT 2 cut(s) 1317, 1700
Asp700I GAANNNNTTC 3 cut(s) 197, 390, 700
Asp718I GGTACC 1 cut(s) 138
AspLEI GCGC 6 cut(s) 93, 524, 603, 1248, 1449, 1654
AspS9I GGNCC 5 cut(s) 1076, 1393, 1586, 1603, 2296
AsuC2I CCSGG 2 cut(s) 1352, 1427
AsuHPI GGTGA 5 cut(s) 92, 295, 1316, 1715, 1837
AvaII GGWCC 3 cut(s) 1076, 1393, 1586
BaeGI GKGCMC 2 cut(s) 1929, 1964
BaeI ACNNNNGTAYC 2 cut(s) 949, 982
BalI TGGCCA 2 cut(s) 507, 680
BamHI GGATCC 1 cut(s) 1476
BanI GGYRCC 3 cut(s) 138, 1245, 1796
BauI CACGAG 3 cut(s) 103, 1104, 2300
BbsI GAAGAC 1 cut(s) 90
BbvI GCAGC 5 cut(s) 478, 1229, 1456, 2177, 2294
BccI CCATC 3 cut(s) 519, 772, 2265
BciT130I CCWGG 3 cut(s) 143, 1080, 1635
BciVI GTATCC 3 cut(s) 1238, 1634, 1954
BcnI CCSGG 2 cut(s) 1352, 1427
BcoDI GTCTC 5 cut(s) 297, 1341, 1882, 2018, 2139
BcuI ACTAGT 1 cut(s) 1223
BfaI CTAG 5 cut(s) 657, 1224, 1236, 2273, 2484
BfmI CTRYAG 2 cut(s) 1442, 2504
BfoI RGCGCY 2 cut(s) 525, 1249
BfuAI ACCTGC 1 cut(s) 1448
BfuI GTATCC 3 cut(s) 1238, 1634, 1954
BisI GCNGC 9 cut(s) 120, 492, 1202, 1215, 1218, 1360, 1445, 2166, 2308
BlpI GCTNAGC 1 cut(s) 2146
BlsI GCNGC 9 cut(s) 121, 493, 1203, 1216, 1219, 1361, 1446, 2167, 2309
Bme1390I CCNGG 5 cut(s) 143, 1080, 1352, 1427, 1635
Bme18I GGWCC 3 cut(s) 1076, 1393, 1586
BmgT120I GGNCC 5 cut(s) 1076, 1393, 1586, 1603, 2296
BmiI GGNNCC 7 cut(s) 140, 1247, 1462, 1478, 1587, 1798, 2297
BmrFI CCNGG 5 cut(s) 143, 1080, 1352, 1427, 1635
BmrI ACTGGG 1 cut(s) 320
BmsI GCATC 3 cut(s) 862, 1857, 2107
BmuI ACTGGG 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 90
BplI GAGNNNNNCTC 6 cut(s) 463, 495, 465, 497, 535, 567
BpmI CTGGAG 3 cut(s) 1538, 2372, 2441
Bpu1102I GCTNAGC 1 cut(s) 2146
BpuEI CTTGAG 6 cut(s) 88, 1532, 1733, 2078, 2252, 2450
BpuMI CCSGG 2 cut(s) 1352, 1427
Bsa29I ATCGAT 1 cut(s) 699
BsaAI YACGTR 2 cut(s) 674, 1369
BsaHI GRCGYC 1 cut(s) 1246
BsaI GGTCTC 2 cut(s) 1341, 2018
BsaJI CCNNGG 3 cut(s) 1241, 1633, 1803
BsaWI WCCGGW 3 cut(s) 1317, 1588, 1700
BsaXI ACNNNNNCTCC 3 cut(s) 32, 1862, 1892
Bsc4I CCNNNNNNNGG 6 cut(s) 48, 118, 1255, 1324, 1426, 2035
Bse118I RCCGGY 4 cut(s) 860, 1317, 1700, 1793
Bse1I ACTGG 5 cut(s) 315, 595, 1736, 2355, 2424
Bse3DI GCAATG 2 cut(s) 1494, 2416
BseAI TCCGGA 1 cut(s) 1588
BseBI CCWGG 3 cut(s) 143, 1080, 1635
BseCI ATCGAT 1 cut(s) 699
BseDI CCNNGG 3 cut(s) 1241, 1633, 1803
BseGI GGATG 4 cut(s) 411, 783, 829, 2122
BseLI CCNNNNNNNGG 6 cut(s) 48, 118, 1255, 1324, 1426, 2035
BseMI GCAATG 2 cut(s) 1494, 2416
BseMII CTCAG 4 cut(s) 1288, 1681, 1748, 1858
BseNI ACTGG 5 cut(s) 315, 595, 1736, 2355, 2424
BseRI GAGGAG 2 cut(s) 10, 22
BseSI GKGCMC 2 cut(s) 1929, 1964
BseX3I CGGCCG 1 cut(s) 2390
BseXI GCAGC 5 cut(s) 478, 1229, 1456, 2177, 2294
Bsh1236I CGCG 7 cut(s) 91, 119, 603, 708, 870, 1449, 2081
Bsh1285I CGRYCG 1 cut(s) 2393
BshFI GGCC 7 cut(s) 507, 680, 1240, 1407, 1605, 2298, 2392
BshNI GGYRCC 3 cut(s) 138, 1245, 1796
BshTI ACCGGT 2 cut(s) 1317, 1700
BshVI ATCGAT 1 cut(s) 699
BsiEI CGRYCG 1 cut(s) 2393
BsiSI CCGG 7 cut(s) 861, 1318, 1351, 1426, 1589, 1701, 1794
BsiWI CGTACG 1 cut(s) 1675
BslFI GGGAC 1 cut(s) 1648
BslI CCNNNNNNNGG 6 cut(s) 48, 118, 1255, 1324, 1426, 2035
BsmAI GTCTC 5 cut(s) 297, 1341, 1882, 2018, 2139
BsmFI GGGAC 1 cut(s) 1648
BsmI GAATGC 4 cut(s) 864, 1051, 2344, 2443
BsnI GGCC 7 cut(s) 507, 680, 1240, 1407, 1605, 2298, 2392
Bso31I GGTCTC 2 cut(s) 1341, 2018
Bsp1286I GDGCHC 2 cut(s) 1929, 1964
Bsp13I TCCGGA 1 cut(s) 1588
Bsp1720I GCTNAGC 1 cut(s) 2146
Bsp19I CCATGG 2 cut(s) 1241, 1803
Bsp68I TCGCGA 1 cut(s) 870
BspACI CCGC 8 cut(s) 119, 541, 1202, 1215, 1360, 1420, 2051, 2079
BspANI GGCC 7 cut(s) 507, 680, 1240, 1407, 1605, 2298, 2392
BspCNI CTCAG 4 cut(s) 1287, 1680, 1749, 1857
BspDI ATCGAT 1 cut(s) 699
BspEI TCCGGA 1 cut(s) 1588
BspFNI CGCG 7 cut(s) 91, 119, 603, 708, 870, 1449, 2081
BspHI TCATGA 1 cut(s) 1063
BspLI GGNNCC 7 cut(s) 140, 1247, 1462, 1478, 1587, 1798, 2297
BspMAI CTGCAG 1 cut(s) 1446
BspMI ACCTGC 1 cut(s) 1448
BspPI GGATC 6 cut(s) 230, 341, 751, 1335, 1471, 1484
BspQI GCTCTTC 1 cut(s) 1587
BspT107I GGYRCC 3 cut(s) 138, 1245, 1796
BspTNI GGTCTC 2 cut(s) 1341, 2018
BsrBI CCGCTC 1 cut(s) 2051
BsrDI GCAATG 2 cut(s) 1494, 2416
BsrFI RCCGGY 4 cut(s) 860, 1317, 1700, 1793
BsrI ACTGG 5 cut(s) 315, 595, 1736, 2355, 2424
BssAI RCCGGY 4 cut(s) 860, 1317, 1700, 1793
BssECI CCNNGG 3 cut(s) 1241, 1633, 1803
BssNI GRCGYC 1 cut(s) 1246
BssSI CACGAG 3 cut(s) 103, 1104, 2300
BssT1I CCWWGG 2 cut(s) 1241, 1803
Bst2BI CACGAG 3 cut(s) 103, 1104, 2300
Bst2UI CCWGG 3 cut(s) 143, 1080, 1635
Bst4CI ACNGT 5 cut(s) 198, 802, 1646, 1733, 1899
Bst6I CTCTTC 5 cut(s) 28, 207, 530, 1587, 2427
BstACI GRCGYC 1 cut(s) 1246
BstBAI YACGTR 2 cut(s) 674, 1369
BstC8I GCNNGC 2 cut(s) 862, 1986
BstDEI CTNAG 7 cut(s) 834, 1274, 1667, 1757, 1784, 1844, 2146
BstDSI CCRYGG 2 cut(s) 1241, 1803
BstF5I GGATG 4 cut(s) 411, 783, 829, 2122
BstFNI CGCG 7 cut(s) 91, 119, 603, 708, 870, 1449, 2081
BstH2I RGCGCY 2 cut(s) 525, 1249
BstHHI GCGC 6 cut(s) 93, 524, 603, 1248, 1449, 1654
BstMAI GTCTC 5 cut(s) 297, 1341, 1882, 2018, 2139
BstMCI CGRYCG 1 cut(s) 2393
BstMWI GCNNNNNNNGC 7 cut(s) 14, 105, 128, 600, 1419, 2078, 2304
BstNI CCWGG 3 cut(s) 143, 1080, 1635
BstNSI RCATGY 4 cut(s) 323, 359, 1691, 2321
BstSCI CCNGG 5 cut(s) 141, 1078, 1350, 1425, 1633
BstSFI CTRYAG 2 cut(s) 1442, 2504
BstSLI GKGCMC 2 cut(s) 1929, 1964
BstUI CGCG 7 cut(s) 91, 119, 603, 708, 870, 1449, 2081
BstV1I GCAGC 5 cut(s) 478, 1229, 1456, 2177, 2294
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 3 cut(s) 222, 1327, 1476
BstXI CCANNNNNNTGG 1 cut(s) 590
BstYI RGATCY 3 cut(s) 222, 1327, 1476
BstZI CGGCCG 1 cut(s) 2390
Bsu15I ATCGAT 1 cut(s) 699
BsuI GTATCC 3 cut(s) 1238, 1634, 1954
BsuRI GGCC 7 cut(s) 507, 680, 1240, 1407, 1605, 2298, 2392
BsuTUI ATCGAT 1 cut(s) 699
BtgI CCRYGG 2 cut(s) 1241, 1803
BtgZI GCGATG 1 cut(s) 885
BtsCI GGATG 4 cut(s) 411, 783, 829, 2122
BtsIMutI CAGTG 2 cut(s) 473, 2348
BtuMI TCGCGA 1 cut(s) 870
BveI ACCTGC 1 cut(s) 1448
Cac8I GCNNGC 2 cut(s) 862, 1986
CciI TCATGA 1 cut(s) 1063
CfoI GCGC 6 cut(s) 93, 524, 603, 1248, 1449, 1654
Cfr10I RCCGGY 4 cut(s) 860, 1317, 1700, 1793
Cfr13I GGNCC 5 cut(s) 1076, 1393, 1586, 1603, 2296
ClaI ATCGAT 1 cut(s) 699
CseI GACGC 1 cut(s) 1292
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 5 cut(s) 55, 139, 1676, 2208, 2477
CspAI ACCGGT 2 cut(s) 1317, 1700
CspCI CAANNNNNGTGG 2 cut(s) 1090, 1125
CviQI GTAC 5 cut(s) 55, 139, 1676, 2208, 2477
DdeI CTNAG 7 cut(s) 834, 1274, 1667, 1757, 1784, 1844, 2146
DinI GGCGCC 1 cut(s) 1247
EaeI YGGCCR 3 cut(s) 505, 678, 2390
EagI CGGCCG 1 cut(s) 2390
Eam1104I CTCTTC 5 cut(s) 28, 207, 530, 1587, 2427
EarI CTCTTC 5 cut(s) 28, 207, 530, 1587, 2427
EciI GGCGGA 1 cut(s) 556
EclXI CGGCCG 1 cut(s) 2390
Eco130I CCWWGG 2 cut(s) 1241, 1803
Eco31I GGTCTC 2 cut(s) 1341, 2018
Eco32I GATATC 2 cut(s) 1860, 2041
Eco47I GGWCC 3 cut(s) 1076, 1393, 1586
Eco47III AGCGCT 1 cut(s) 523
Eco52I CGGCCG 1 cut(s) 2390
Eco57I CTGAAG 2 cut(s) 58, 2051
EcoO109I RGGNCCY 1 cut(s) 1603
EcoRI GAATTC 2 cut(s) 842, 1973
EcoRII CCWGG 3 cut(s) 141, 1078, 1633
EcoRV GATATC 2 cut(s) 1860, 2041
EcoT14I CCWWGG 2 cut(s) 1241, 1803
EcoT22I ATGCAT 1 cut(s) 1415
EgeI GGCGCC 1 cut(s) 1247
EheI GGCGCC 1 cut(s) 1247
ErhI CCWWGG 2 cut(s) 1241, 1803
FaqI GGGAC 1 cut(s) 1648
FauI CCCGC 1 cut(s) 2086
FblI GTMKAC 2 cut(s) 1117, 2055
Fnu4HI GCNGC 9 cut(s) 120, 492, 1202, 1215, 1218, 1360, 1445, 2166, 2308
FokI GGATG 4 cut(s) 398, 790, 816, 2129
Fsp4HI GCNGC 9 cut(s) 120, 492, 1202, 1215, 1218, 1360, 1445, 2166, 2308
FspBI CTAG 5 cut(s) 657, 1224, 1236, 2273, 2484
GlaI GCGC 6 cut(s) 92, 523, 602, 1247, 1448, 1653
GluI GCNGC 9 cut(s) 120, 492, 1202, 1215, 1218, 1360, 1445, 2166, 2308
GsuI CTGGAG 3 cut(s) 1538, 2372, 2441
HaeII RGCGCY 2 cut(s) 525, 1249
HaeIII GGCC 7 cut(s) 507, 680, 1240, 1407, 1605, 2298, 2392
HapII CCGG 7 cut(s) 861, 1318, 1351, 1426, 1589, 1701, 1794
HgaI GACGC 1 cut(s) 1292
HhaI GCGC 6 cut(s) 93, 524, 603, 1248, 1449, 1654
Hin1I GRCGYC 1 cut(s) 1246
Hin6I GCGC 6 cut(s) 91, 522, 601, 1246, 1447, 1652
HinP1I GCGC 6 cut(s) 91, 522, 601, 1246, 1447, 1652
HincII GTYRAC 3 cut(s) 958, 1291, 1997
HindII GTYRAC 3 cut(s) 958, 1291, 1997
HpaI GTTAAC 1 cut(s) 1291
HpaII CCGG 7 cut(s) 861, 1318, 1351, 1426, 1589, 1701, 1794
HphI GGTGA 5 cut(s) 92, 295, 1316, 1715, 1837
Hpy166II GTNNAC 8 cut(s) 884, 958, 1018, 1118, 1291, 1895, 1997, 2056
Hpy188I TCNGA 5 cut(s) 396, 577, 1670, 2064, 2324
Hpy8I GTNNAC 8 cut(s) 884, 958, 1018, 1118, 1291, 1895, 1997, 2056
Hpy99I CGWCG 2 cut(s) 62, 2398
HpyAV CCTTC 4 cut(s) 360, 956, 1628, 2490
HpyCH4III ACNGT 5 cut(s) 198, 802, 1646, 1733, 1899
HpyCH4IV ACGT 9 cut(s) 60, 342, 439, 673, 1114, 1368, 1386, 1747, 2210
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 105, 128, 600, 1419, 2078, 2304
HpyF3I CTNAG 7 cut(s) 834, 1274, 1667, 1757, 1784, 1844, 2146
HpySE526I ACGT 9 cut(s) 60, 342, 439, 673, 1114, 1368, 1386, 1747, 2210
Hsp92I GRCGYC 1 cut(s) 1246
HspAI GCGC 6 cut(s) 91, 522, 601, 1246, 1447, 1652
KasI GGCGCC 1 cut(s) 1245
Kpn2I TCCGGA 1 cut(s) 1588
KpnI GGTACC 1 cut(s) 142
KroI GCCGGC 1 cut(s) 860
KroNI GCCGGC 1 cut(s) 862
KspAI GTTAAC 1 cut(s) 1291
LguI GCTCTTC 1 cut(s) 1587
LmnI GCTCC 3 cut(s) 1519, 2048, 2173
Lsp1109I GCAGC 5 cut(s) 478, 1229, 1456, 2177, 2294
LweI GCATC 3 cut(s) 862, 1857, 2107
MabI ACCWGGT 1 cut(s) 141
MaeI CTAG 5 cut(s) 657, 1224, 1236, 2273, 2484
MaeII ACGT 9 cut(s) 60, 342, 439, 673, 1114, 1368, 1386, 1747, 2210
MaeIII GTNAC 3 cut(s) 1191, 1696, 1789
MbiI CCGCTC 1 cut(s) 2051
MfeI CAATTG 1 cut(s) 1536
MflI RGATCY 3 cut(s) 222, 1327, 1476
MhlI GDGCHC 2 cut(s) 1929, 1964
MlsI TGGCCA 2 cut(s) 507, 680
MluNI TGGCCA 2 cut(s) 507, 680
Mly113I GGCGCC 1 cut(s) 1246
MlyI GAGTC 5 cut(s) 224, 1264, 1526, 1670, 1680
MmeI TCCRAC 5 cut(s) 673, 745, 1036, 1752, 2482
Mox20I TGGCCA 2 cut(s) 507, 680
Mph1103I ATGCAT 1 cut(s) 1415
MroI TCCGGA 1 cut(s) 1588
MroNI GCCGGC 1 cut(s) 860
MroXI GAANNNNTTC 3 cut(s) 197, 390, 700
MscI TGGCCA 2 cut(s) 507, 680
MseI TTAA 6 cut(s) 125, 618, 630, 1290, 1901, 1991
MslI CAYNNNNRTG 3 cut(s) 1635, 1692, 1802
Msp20I TGGCCA 2 cut(s) 507, 680
MspI CCGG 7 cut(s) 861, 1318, 1351, 1426, 1589, 1701, 1794
MspR9I CCNGG 5 cut(s) 143, 1080, 1352, 1427, 1635
MunI CAATTG 1 cut(s) 1536
Mva1269I GAATGC 4 cut(s) 864, 1051, 2344, 2443
MvaI CCWGG 3 cut(s) 143, 1080, 1635
MvnI CGCG 7 cut(s) 91, 119, 603, 708, 870, 1449, 2081
MwoI GCNNNNNNNGC 7 cut(s) 14, 105, 128, 600, 1419, 2078, 2304
NaeI GCCGGC 1 cut(s) 862
NarI GGCGCC 1 cut(s) 1246
NciI CCSGG 2 cut(s) 1352, 1427
NcoI CCATGG 2 cut(s) 1241, 1803
NgoMIV GCCGGC 1 cut(s) 860
NlaIV GGNNCC 7 cut(s) 140, 1247, 1462, 1478, 1587, 1798, 2297
NmuCI GTSAC 2 cut(s) 1191, 1696
NruI TCGCGA 1 cut(s) 870
NsiI ATGCAT 1 cut(s) 1415
NspI RCATGY 4 cut(s) 323, 359, 1691, 2321
OliI CACNNNNGTG 1 cut(s) 1635
PagI TCATGA 1 cut(s) 1063
PciI ACATGT 2 cut(s) 319, 1687
PciSI GCTCTTC 1 cut(s) 1587
PcsI WCGNNNNNNNCGW 4 cut(s) 705, 966, 1111, 2307
PctI GAATGC 4 cut(s) 864, 1051, 2344, 2443
PdiI GCCGGC 1 cut(s) 862
PdmI GAANNNNTTC 3 cut(s) 197, 390, 700
PfeI GAWTC 6 cut(s) 257, 696, 701, 1131, 1152, 2065
Pfl23II CGTACG 1 cut(s) 1675
PflMI CCANNNNNTGG 1 cut(s) 48
PfoI TCCNGGA 1 cut(s) 1078
PinAI ACCGGT 2 cut(s) 1317, 1700
PkrI GCNGC 9 cut(s) 121, 493, 1203, 1216, 1219, 1361, 1446, 2167, 2309
PleI GAGTC 5 cut(s) 223, 1264, 1526, 1669, 1679
PluTI GGCGCC 1 cut(s) 1249
PpsI GAGTC 5 cut(s) 223, 1264, 1526, 1669, 1679
Ppu21I YACGTR 2 cut(s) 674, 1369
PscI ACATGT 2 cut(s) 319, 1687
PsiI TTATAA 2 cut(s) 819, 2366
Psp1406I AACGTT 1 cut(s) 1747
Psp6I CCWGG 3 cut(s) 141, 1078, 1633
PspGI CCWGG 3 cut(s) 141, 1078, 1633
PspLI CGTACG 1 cut(s) 1675
PspN4I GGNNCC 7 cut(s) 140, 1247, 1462, 1478, 1587, 1798, 2297
PspPI GGNCC 5 cut(s) 1076, 1393, 1586, 1603, 2296
PstI CTGCAG 1 cut(s) 1446
PsuI RGATCY 3 cut(s) 222, 1327, 1476
RruI TCGCGA 1 cut(s) 870
RsaI GTAC 5 cut(s) 56, 140, 1677, 2209, 2478
RsaNI GTAC 5 cut(s) 55, 139, 1676, 2208, 2477
RseI CAYNNNNRTG 3 cut(s) 1635, 1692, 1802
SapI GCTCTTC 1 cut(s) 1587
SaqAI TTAA 6 cut(s) 125, 618, 630, 1290, 1901, 1991
SatI GCNGC 9 cut(s) 120, 492, 1202, 1215, 1218, 1360, 1445, 2166, 2308
Sau96I GGNCC 5 cut(s) 1076, 1393, 1586, 1603, 2296
SchI GAGTC 5 cut(s) 224, 1264, 1526, 1670, 1680
ScrFI CCNGG 5 cut(s) 143, 1080, 1352, 1427, 1635
SduI GDGCHC 2 cut(s) 1929, 1964
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 3 cut(s) 862, 1857, 2107
SfcI CTRYAG 2 cut(s) 1442, 2504
SfoI GGCGCC 1 cut(s) 1247
SinI GGWCC 3 cut(s) 1076, 1393, 1586
SmiMI CAYNNNNRTG 3 cut(s) 1635, 1692, 1802
SmlI CTYRAG 6 cut(s) 67, 1511, 1712, 2093, 2267, 2465
SmoI CTYRAG 6 cut(s) 67, 1511, 1712, 2093, 2267, 2465
SpeI ACTAGT 1 cut(s) 1223
SsiI CCGC 8 cut(s) 119, 541, 1202, 1215, 1360, 1420, 2051, 2079
SspDI GGCGCC 1 cut(s) 1245
SspI AATATT 3 cut(s) 333, 640, 1817
SspMI CTAG 5 cut(s) 657, 1224, 1236, 2273, 2484
StyD4I CCNGG 5 cut(s) 141, 1078, 1350, 1425, 1633
StyI CCWWGG 2 cut(s) 1241, 1803
TaaI ACNGT 5 cut(s) 198, 802, 1646, 1733, 1899
TaiI ACGT 9 cut(s) 63, 345, 442, 676, 1117, 1371, 1389, 1750, 2213
TaqI TCGA 9 cut(s) 210, 218, 260, 699, 969, 991, 1380, 1659, 1743
TaqII GACCGA 2 cut(s) 1143, 1164
TauI GCSGC 4 cut(s) 122, 1204, 1217, 1362
TfiI GAWTC 6 cut(s) 257, 696, 701, 1131, 1152, 2065
Tru1I TTAA 6 cut(s) 125, 618, 630, 1290, 1901, 1991
Tru9I TTAA 6 cut(s) 125, 618, 630, 1290, 1901, 1991
TscAI CASTG 2 cut(s) 473, 2355
TseFI GTSAC 2 cut(s) 1191, 1696
TseI GCWGC 5 cut(s) 491, 1217, 1444, 2165, 2307
Tsp45I GTSAC 2 cut(s) 1191, 1696
TspDTI ATGAA 3 cut(s) 604, 818, 2146
TspGWI ACGGA 3 cut(s) 191, 387, 404
TspRI CASTG 2 cut(s) 473, 2355
Van91I CCANNNNNTGG 1 cut(s) 48
VpaK11BI GGWCC 3 cut(s) 1076, 1393, 1586
XapI RAATTY 8 cut(s) 496, 568, 768, 825, 842, 1173, 1973, 2492
XceI RCATGY 4 cut(s) 323, 359, 1691, 2321
XcmI CCANNNNNNNNNTGG 2 cut(s) 587, 2221
XmiI GTMKAC 2 cut(s) 1117, 2055
XmnI GAANNNNTTC 3 cut(s) 197, 390, 700
XspI CTAG 5 cut(s) 657, 1224, 1236, 2273, 2484
Zsp2I ATGCAT 1 cut(s) 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.