MD02G1052300.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013733
Physical Location & Seq
Reverse (-)
4287287 .. 4288396
1110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1052300.v1.1.491

Sequence Viewer

Length: 984 bp
ATGAATTTGTTTCTTTTATTGAAGTTGATCACAAACTCACATACTAGCTATTGCGAAGCACAGCGTATTAGAGAAATTGTTGACAAGATTATTCCGGAATGGCTTCGGAGAGCAAACAAATTACATGTGGCCAATCACCTGGTTGGAATCAATTCTCGCGTTCAAGATATTATCAGTTATCTTTCAAGTGGTGGATCAGATGATGTTCGCATGGTTGGAATTTGGGGGATGGGTGGATTGGGTAAAACAACAGCTGCCAAAGCCATTTATAACAAAATTCATCATGAGTTCCAATTCAAAAGTTTCCTTGACAACGTTAGTGAAAAAGATCTGGTTGTTTCGCAAAAAAAACTTGTTTCTGACATCTTGAAACAGACCAAGCCTGAGATAACCAGTGTTGATGAAGGTATCGGCCTGATAAAATATCATCTCCAACGTAGAAGCGTACTTGTCATTATTGACAATGTAGACAAAATGAAACAACTAAATGCAATAGCTGGAAATTGTGATTGGTTTGGCTTAGGAAGTAGAATTATCATAACGACACGAGATGAACATTTACTACGCCAAGTTGGAGTGGACAAGATATATCCGCTTCATAAAATGAATGAAGAAGAAGCTCTGGAGCTCTTTAGTTGGCATGCCTTTAGAAATTGTTGGCCTAATGAAGGATATCTTGAACTCTCAAGGAAGATCGTTTCTTACTGTGGAGGTTTGCCACTAGCACTTGAAGTTTTAGGCACTTTTTTAATTGGAAGAACCATAGCAGAGTGGGAAAATCAATTGGTAAAATTGAAAACAACTCCTGAAGGAGAAATAATACAACGACTAAGAATAAGCTTTGAAGGGCTAGATGATAAACAGAAGGCTATATTCCTTGACATATCTTGTTTCTTCATTGGATGGGATAATGACTATGTCACAAAAATATTAGATGGATGTGACTTTTTTGCGACAATAGGAATGAGTGTCCTTCGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

328

Amino Acids

37.28

Weight (kDa)

6.76

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 53 - 218 2.6e-19 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 270
AccI GTMKAC 1 cut(s) 468
AccII CGCG 1 cut(s) 159
AccIII TCCGGA 1 cut(s) 94
AciI CCGC 1 cut(s) 593
AclI AACGTT 1 cut(s) 315
AclWI GGATC 1 cut(s) 202
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 3 cut(s) 4, 219, 276
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 1 cut(s) 447
AfiI CCNNNNNNNGG 1 cut(s) 668
AflIII ACRYGT 1 cut(s) 124
AgsI TTSAA 9 cut(s) 22, 164, 186, 298, 370, 680, 731, 796, 845
AjnI CCWGG 1 cut(s) 138
AjuI GAANNNNNNNTTGG 2 cut(s) 493, 525
AluBI AGCT 6 cut(s) 48, 254, 497, 620, 628, 840
AluI AGCT 6 cut(s) 48, 254, 497, 620, 628, 840
Alw21I GWGCWC 1 cut(s) 630
AlwI GGATC 1 cut(s) 202
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 3 cut(s) 129, 412, 659
ApeKI GCWGC 1 cut(s) 254
ApoI RAATTY 3 cut(s) 4, 219, 276
Asp700I GAANNNNTTC 2 cut(s) 102, 151
AsuHPI GGTGA 1 cut(s) 128
BalI TGGCCA 1 cut(s) 131
BanII GRGCYC 1 cut(s) 630
BauI CACGAG 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 241
BccI CCATC 3 cut(s) 223, 897, 929
BciT130I CCWGG 1 cut(s) 140
BclI TGATCA 1 cut(s) 27
BfaI CTAG 3 cut(s) 45, 722, 851
BglII AGATCT 1 cut(s) 328
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 140
BpmI CTGGAG 1 cut(s) 644
Bpu10I CCTNAGC 1 cut(s) 520
BpuEI CTTGAG 1 cut(s) 670
BsaWI WCCGGW 1 cut(s) 94
Bsc4I CCNNNNNNNGG 1 cut(s) 668
Bse1I ACTGG 1 cut(s) 393
BseAI TCCGGA 1 cut(s) 94
BseBI CCWGG 1 cut(s) 140
BseGI GGATG 3 cut(s) 234, 908, 944
BseLI CCNNNNNNNGG 1 cut(s) 668
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 393
BseXI GCAGC 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 3 cut(s) 131, 414, 661
BsiHKAI GWGCWC 1 cut(s) 630
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 668
BsnI GGCC 3 cut(s) 131, 414, 661
Bsp1286I GDGCHC 1 cut(s) 630
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 4 cut(s) 27, 194, 328, 693
BspACI CCGC 1 cut(s) 593
BspANI GGCC 3 cut(s) 131, 414, 661
BspCNI CTCAG 1 cut(s) 376
BspEI TCCGGA 1 cut(s) 94
BspFNI CGCG 1 cut(s) 159
BspHI TCATGA 1 cut(s) 283
BspPI GGATC 1 cut(s) 202
BsrI ACTGG 1 cut(s) 393
BssMI GATC 4 cut(s) 27, 194, 328, 693
BssSI CACGAG 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 707
BstC8I GCNNGC 1 cut(s) 642
BstDEI CTNAG 3 cut(s) 384, 520, 830
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 3 cut(s) 234, 908, 944
BstFNI CGCG 1 cut(s) 159
BstKTI GATC 4 cut(s) 30, 197, 331, 696
BstMBI GATC 4 cut(s) 27, 194, 328, 693
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 2 cut(s) 128, 644
BstSCI CCNGG 1 cut(s) 138
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 241
BstX2I RGATCY 1 cut(s) 328
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 3 cut(s) 131, 414, 661
BtsCI GGATG 3 cut(s) 234, 908, 944
BtsIMutI CAGTG 1 cut(s) 400
Cac8I GCNNGC 1 cut(s) 642
CciI TCATGA 1 cut(s) 283
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 1 cut(s) 446
CviAII CATG 4 cut(s) 125, 211, 284, 641
CviQI GTAC 1 cut(s) 446
DdeI CTNAG 3 cut(s) 384, 520, 830
DpnI GATC 4 cut(s) 29, 196, 330, 695
DpnII GATC 4 cut(s) 27, 194, 328, 693
EaeI YGGCCR 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 628
Eco24I GRGCYC 1 cut(s) 630
Eco32I GATATC 1 cut(s) 674
Eco53kI GAGCTC 1 cut(s) 628
Eco57I CTGAAG 1 cut(s) 828
EcoICRI GAGCTC 1 cut(s) 628
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoRII CCWGG 1 cut(s) 138
EcoRV GATATC 1 cut(s) 674
EcoT38I GRGCYC 1 cut(s) 630
FaeI CATG 4 cut(s) 128, 214, 287, 644
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FatI CATG 4 cut(s) 124, 210, 283, 640
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 3 cut(s) 241, 915, 951
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 1 cut(s) 255
FspBI CTAG 3 cut(s) 45, 722, 851
GluI GCNGC 1 cut(s) 255
GsuI CTGGAG 1 cut(s) 644
HaeIII GGCC 3 cut(s) 131, 414, 661
HapII CCGG 1 cut(s) 95
Hin1II CATG 4 cut(s) 128, 214, 287, 644
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 838
HinfI GANTC 1 cut(s) 147
HpaII CCGG 1 cut(s) 95
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 3 cut(s) 82, 469, 580
Hpy188I TCNGA 3 cut(s) 108, 199, 361
Hpy188III TCNNGA 8 cut(s) 95, 164, 284, 367, 623, 677, 806, 977
Hpy8I GTNNAC 3 cut(s) 82, 469, 580
HpyAV CCTTC 6 cut(s) 398, 662, 803, 839, 859, 983
HpyCH4III ACNGT 1 cut(s) 707
HpyCH4IV ACGT 2 cut(s) 315, 436
HpyCH4V TGCA 1 cut(s) 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 3 cut(s) 384, 520, 830
HpySE526I ACGT 2 cut(s) 315, 436
Hsp92II CATG 4 cut(s) 128, 214, 287, 644
Kpn2I TCCGGA 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 4 cut(s) 27, 194, 328, 693
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 1 cut(s) 241
MabI ACCWGGT 1 cut(s) 138
MaeI CTAG 3 cut(s) 45, 722, 851
MaeII ACGT 2 cut(s) 315, 436
MaeIII GTNAC 2 cut(s) 919, 941
MalI GATC 4 cut(s) 29, 196, 330, 695
MboI GATC 4 cut(s) 27, 194, 328, 693
MboII GAAGA 5 cut(s) 623, 626, 703, 768, 886
MfeI CAATTG 1 cut(s) 782
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 1 cut(s) 630
MlsI TGGCCA 1 cut(s) 131
MluNI TGGCCA 1 cut(s) 131
MmeI TCCRAC 4 cut(s) 124, 196, 457, 553
MnlI CCTC 1 cut(s) 704
Mox20I TGGCCA 1 cut(s) 131
MroI TCCGGA 1 cut(s) 94
MroXI GAANNNNTTC 2 cut(s) 102, 151
MscI TGGCCA 1 cut(s) 131
MseI TTAA 1 cut(s) 749
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 140
MunI CAATTG 1 cut(s) 782
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 260
NdeII GATC 4 cut(s) 27, 194, 328, 693
NlaIII CATG 4 cut(s) 128, 214, 287, 644
NmuCI GTSAC 2 cut(s) 919, 941
NspI RCATGY 2 cut(s) 128, 644
PaeI GCATGC 1 cut(s) 644
PagI TCATGA 1 cut(s) 283
PciI ACATGT 1 cut(s) 124
PdmI GAANNNNTTC 2 cut(s) 102, 151
PfeI GAWTC 1 cut(s) 147
PflFI GACNNNGTC 1 cut(s) 917
PkrI GCNGC 1 cut(s) 256
PscI ACATGT 1 cut(s) 124
PsiI TTATAA 1 cut(s) 270
Psp124BI GAGCTC 1 cut(s) 630
Psp1406I AACGTT 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PsrI GAACNNNNNNTAC 2 cut(s) 546, 578
PsuI RGATCY 1 cut(s) 328
PsyI GACNNNGTC 1 cut(s) 917
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 1 cut(s) 447
RsaNI GTAC 1 cut(s) 446
SacI GAGCTC 1 cut(s) 630
SaqAI TTAA 1 cut(s) 749
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 4 cut(s) 27, 194, 328, 693
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 630
SexAI ACCWGGT 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
SphI GCATGC 1 cut(s) 644
SsiI CCGC 1 cut(s) 593
SspI AATATT 1 cut(s) 930
SspMI CTAG 3 cut(s) 45, 722, 851
SstI GAGCTC 1 cut(s) 630
StyD4I CCNGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 707
TaiI ACGT 2 cut(s) 318, 439
TfiI GAWTC 1 cut(s) 147
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 2 cut(s) 919, 941
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 2 cut(s) 919, 941
TspRI CASTG 1 cut(s) 400
Tth111I GACNNNGTC 1 cut(s) 917
XagI CCTNNNNNAGG 1 cut(s) 666
XapI RAATTY 3 cut(s) 4, 219, 276
XceI RCATGY 2 cut(s) 128, 644
XmiI GTMKAC 1 cut(s) 468
XmnI GAANNNNTTC 2 cut(s) 102, 151
XspI CTAG 3 cut(s) 45, 722, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.