MD02G1056000.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013733
Physical Location & Seq
Reverse (-)
4526059 .. 4531857
5799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1056000.v1.1.491

Sequence Viewer

Length: 924 bp
ATGACAACCCACGAAGCCTCCTCTTCATCCTCCTCCAAGTCAAAACTTTGGAATTACGACGTGTTCTTGAGCTTCAGCGGTGAAGACACACGCAATGGCTTCACGGACCACCTCCATGCGGCATTAACAGACAAGGGATACCAGGCTTATATTGATGAGGATGGTCTAAAAAGAGGGGAAGAAATACAAAAAGAACTGGAACAGGCAATCGAAAAATCGAAGATCTCTATCATTGTCTTCTCAGAGAGGTATGCAGATTCAAGTTGGTGTCTTAACGAGCTGGTGAAGATCATGGAGTGCAGAGACAAACTGGGGCGACATGTTTTGCCAATATTCTATCACATTGATCCTTCACATGTCAGGAAGCAGAATGGAGTTTTAGCCCAAGCATTTAAGAAGCACAAAAAGAACATCCGTAAAGAAAAAGATGACAAGAAACGTGAAGAAAACCGAGAAAGGGTAAAGCAGTGGAGAAAGGCTCTTAGAGAAGCTGCAGAGTTAGCCGAAGAATTTCAGGAGCACGAAGAGGGCATGGGTGAAGAAAAGGATGACAAGAAACGCAAGAAACGTGAAGCTAAACAAGAAGGGGTAAAGCAGTGGGAAGAGGTTCTTACAAAAGCTGCAAATTTGTCTGGCTACCATCTTCAAATCACTGATAATCGGCGCGAAGCAAAGCTTATTAGAGAAATTGTTGACAAGATTATCCCGGAATGGCTTCCGAGCGCAGAAAAATTACATGTGGCCAAGCACCCAGTTGGAATATATAATCGCATTCAAGATATCATCACTTATCTTTCAAATGGTGGATCAGATGTTGTTCGCATGGTTGGAATTTGGGGTATGGGTGGATTGGGTAAAACAACAGCTGCCAAAGCCATTTATAACCAAATTCATCATAAGTTCCAATTCAAAAACCGAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

308

Amino Acids

35.61

Weight (kDa)

9.09

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 171 6.2e-47 TIR domain
TIR_2 PF13676 21 - 117 1.5e-18 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
AccII CGCG 1 cut(s) 666
AciI CCGC 2 cut(s) 78, 119
AclWI GGATC 2 cut(s) 341, 814
AcoI YGGCCR 1 cut(s) 741
AcsI RAATTY 4 cut(s) 509, 625, 831, 888
AcuI CTGAAG 1 cut(s) 58
AfiI CCNNNNNNNGG 2 cut(s) 118, 457
AflIII ACRYGT 4 cut(s) 60, 319, 355, 736
AgsI TTSAA 5 cut(s) 261, 647, 776, 798, 910
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 7 cut(s) 72, 280, 491, 575, 620, 676, 866
AluI AGCT 7 cut(s) 72, 280, 491, 575, 620, 676, 866
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 2 cut(s) 341, 814
AoxI GGCC 1 cut(s) 741
ApeKI GCWGC 3 cut(s) 491, 620, 866
ApoI RAATTY 4 cut(s) 509, 625, 831, 888
ArsI GACNNNNNNTTYG 1 cut(s) 903
Asp700I GAANNNNTTC 3 cut(s) 510, 606, 714
AspLEI GCGC 2 cut(s) 666, 725
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 1 cut(s) 707
AsuHPI GGTGA 3 cut(s) 92, 295, 548
AvaII GGWCC 1 cut(s) 106
BalI TGGCCA 1 cut(s) 743
BbsI GAAGAC 2 cut(s) 90, 229
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 3 cut(s) 478, 607, 853
BccI CCATC 2 cut(s) 155, 648
BciT130I CCWGG 1 cut(s) 143
BciVI GTATCC 1 cut(s) 131
BcnI CCSGG 1 cut(s) 707
BcoDI GTCTC 1 cut(s) 297
BfmI CTRYAG 1 cut(s) 492
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 1 cut(s) 222
BisI GCNGC 4 cut(s) 120, 492, 621, 867
BlsI GCNGC 4 cut(s) 121, 493, 622, 868
Bme1390I CCNGG 2 cut(s) 143, 707
Bme18I GGWCC 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 106
BmrFI CCNGG 2 cut(s) 143, 707
BmrI ACTGGG 2 cut(s) 320, 746
BmuI ACTGGG 2 cut(s) 320, 746
BpiI GAAGAC 2 cut(s) 90, 229
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 707
BsaBI GATNNNNATC 1 cut(s) 227
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 457
Bse1I ACTGG 3 cut(s) 201, 315, 752
Bse3DI GCAATG 1 cut(s) 100
Bse8I GATNNNNATC 1 cut(s) 227
BseBI CCWGG 1 cut(s) 143
BseGI GGATG 4 cut(s) 26, 166, 411, 553
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 2 cut(s) 118, 457
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 201, 315, 752
BseRI GAGGAG 2 cut(s) 10, 22
BseXI GCAGC 3 cut(s) 478, 607, 853
BsgI GTGCAG 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 666
BshFI GGCC 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 522
BsiSI CCGG 1 cut(s) 707
BslI CCNNNNNNNGG 2 cut(s) 118, 457
BsmAI GTCTC 1 cut(s) 297
BsmI GAATGC 1 cut(s) 771
BsnI GGCC 1 cut(s) 743
Bsp1286I GDGCHC 1 cut(s) 522
Bsp143I GATC 4 cut(s) 222, 288, 346, 806
BspACI CCGC 2 cut(s) 78, 119
BspANI GGCC 1 cut(s) 743
BspCNI CTCAG 1 cut(s) 254
BspFNI CGCG 1 cut(s) 666
BspMAI CTGCAG 1 cut(s) 496
BspPI GGATC 2 cut(s) 341, 814
BsrDI GCAATG 1 cut(s) 100
BsrI ACTGG 3 cut(s) 201, 315, 752
BssMI GATC 4 cut(s) 222, 288, 346, 806
Bst2UI CCWGG 1 cut(s) 143
Bst6I CTCTTC 3 cut(s) 28, 519, 597
BstDEI CTNAG 2 cut(s) 241, 482
BstF5I GGATG 4 cut(s) 26, 166, 411, 553
BstFNI CGCG 1 cut(s) 666
BstHHI GCGC 2 cut(s) 666, 725
BstKTI GATC 4 cut(s) 225, 291, 349, 809
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 4 cut(s) 222, 288, 346, 806
BstMWI GCNNNNNNNGC 2 cut(s) 500, 872
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 3 cut(s) 323, 359, 740
BstSCI CCNGG 2 cut(s) 141, 705
BstSFI CTRYAG 1 cut(s) 492
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 3 cut(s) 478, 607, 853
BstV2I GAAGAC 2 cut(s) 90, 229
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 743
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 4 cut(s) 26, 166, 411, 553
BtsI GCAGTG 2 cut(s) 473, 602
BtsIMutI CAGTG 3 cut(s) 473, 602, 651
CfoI GCGC 2 cut(s) 666, 725
Cfr13I GGNCC 1 cut(s) 106
CviAII CATG 7 cut(s) 116, 292, 320, 356, 532, 737, 823
DdeI CTNAG 2 cut(s) 241, 482
DpnI GATC 4 cut(s) 224, 290, 348, 808
DpnII GATC 4 cut(s) 222, 288, 346, 806
EaeI YGGCCR 1 cut(s) 741
Eam1104I CTCTTC 3 cut(s) 28, 519, 597
EarI CTCTTC 3 cut(s) 28, 519, 597
Eco32I GATATC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 141
EcoRV GATATC 1 cut(s) 781
FaeI CATG 7 cut(s) 119, 295, 323, 359, 535, 740, 826
FalI AAGNNNNNCTT 4 cut(s) 594, 626, 660, 692
FatI CATG 7 cut(s) 115, 291, 319, 355, 531, 736, 822
Fnu4HI GCNGC 4 cut(s) 120, 492, 621, 867
FokI GGATG 4 cut(s) 13, 173, 398, 560
Fsp4HI GCNGC 4 cut(s) 120, 492, 621, 867
GlaI GCGC 2 cut(s) 665, 724
GluI GCNGC 4 cut(s) 120, 492, 621, 867
HaeIII GGCC 1 cut(s) 743
HapII CCGG 1 cut(s) 707
HhaI GCGC 2 cut(s) 666, 725
Hin1II CATG 7 cut(s) 119, 295, 323, 359, 535, 740, 826
Hin6I GCGC 2 cut(s) 664, 723
HinP1I GCGC 2 cut(s) 664, 723
HincII GTYRAC 1 cut(s) 694
HindII GTYRAC 1 cut(s) 694
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 2 cut(s) 257, 918
HpaII CCGG 1 cut(s) 707
HphI GGTGA 3 cut(s) 92, 295, 548
Hpy166II GTNNAC 1 cut(s) 694
Hpy188I TCNGA 3 cut(s) 244, 720, 811
Hpy188III TCNNGA 4 cut(s) 67, 361, 515, 776
Hpy8I GTNNAC 1 cut(s) 694
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 2 cut(s) 360, 578
HpyCH4IV ACGT 3 cut(s) 60, 439, 568
HpyCH4V TGCA 4 cut(s) 254, 300, 494, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 500, 872
HpyF3I CTNAG 2 cut(s) 241, 482
HpySE526I ACGT 3 cut(s) 60, 439, 568
Hsp92II CATG 7 cut(s) 119, 295, 323, 359, 535, 740, 826
HspAI GCGC 2 cut(s) 664, 723
Kzo9I GATC 4 cut(s) 222, 288, 346, 806
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 3 cut(s) 478, 607, 853
MaeII ACGT 3 cut(s) 60, 439, 568
MalI GATC 4 cut(s) 224, 290, 348, 808
MboI GATC 4 cut(s) 222, 288, 346, 806
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 522
MlsI TGGCCA 1 cut(s) 743
MluCI AATT 8 cut(s) 52, 509, 625, 687, 731, 831, 888, 905
MluNI TGGCCA 1 cut(s) 743
MmeI TCCRAC 2 cut(s) 736, 808
Mox20I TGGCCA 1 cut(s) 743
MroXI GAANNNNTTC 3 cut(s) 510, 606, 714
MscI TGGCCA 1 cut(s) 743
MseI TTAA 3 cut(s) 125, 273, 393
MslI CAYNNNNRTG 1 cut(s) 114
Msp20I TGGCCA 1 cut(s) 743
MspA1I CMGCKG 2 cut(s) 78, 866
MspI CCGG 1 cut(s) 707
MspR9I CCNGG 2 cut(s) 143, 707
Mva1269I GAATGC 1 cut(s) 771
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 2 cut(s) 500, 872
NciI CCSGG 1 cut(s) 707
NdeII GATC 4 cut(s) 222, 288, 346, 806
NlaIII CATG 7 cut(s) 119, 295, 323, 359, 535, 740, 826
NspI RCATGY 3 cut(s) 323, 359, 740
PciI ACATGT 3 cut(s) 319, 355, 736
PcsI WCGNNNNNNNCGW 1 cut(s) 565
PctI GAATGC 1 cut(s) 771
PdmI GAANNNNTTC 3 cut(s) 510, 606, 714
PfeI GAWTC 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 705
PkrI GCNGC 4 cut(s) 121, 493, 622, 868
PscI ACATGT 3 cut(s) 319, 355, 736
PsiI TTATAA 1 cut(s) 882
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspPI GGNCC 1 cut(s) 106
PstI CTGCAG 1 cut(s) 496
PsuI RGATCY 1 cut(s) 222
PvuII CAGCTG 1 cut(s) 866
RseI CAYNNNNRTG 1 cut(s) 114
SaqAI TTAA 3 cut(s) 125, 273, 393
SatI GCNGC 4 cut(s) 120, 492, 621, 867
Sau3AI GATC 4 cut(s) 222, 288, 346, 806
Sau96I GGNCC 1 cut(s) 106
ScrFI CCNGG 2 cut(s) 143, 707
SduI GDGCHC 1 cut(s) 522
SfcI CTRYAG 1 cut(s) 492
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 8 cut(s) 52, 509, 625, 687, 731, 831, 888, 905
SsiI CCGC 2 cut(s) 78, 119
SspI AATATT 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 141, 705
TaiI ACGT 3 cut(s) 63, 442, 571
TaqI TCGA 2 cut(s) 210, 218
TasI AATT 8 cut(s) 52, 509, 625, 687, 731, 831, 888, 905
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 3 cut(s) 125, 273, 393
Tru9I TTAA 3 cut(s) 125, 273, 393
TscAI CASTG 3 cut(s) 473, 602, 658
TseI GCWGC 3 cut(s) 491, 620, 866
TspDTI ATGAA 2 cut(s) 15, 881
TspGWI ACGGA 2 cut(s) 119, 404
TspRI CASTG 3 cut(s) 473, 602, 658
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 4 cut(s) 509, 625, 831, 888
XceI RCATGY 3 cut(s) 323, 359, 740
XmnI GAANNNNTTC 3 cut(s) 510, 606, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.