MD02G1063200.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013733
Physical Location & Seq
Forward (+)
5059738 .. 5062581
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1063200.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGACAGCCCGCGAAGCCGCCTCTTCATCCTCCTCCATGTCAAAACTTTGGAATCGCGACGTGTTCTTGAGCTTCAGAGGCGAAGACACGCGCAGTGGCTTTACGGGCCACCTCCACGCAGCATTAACAGACAGGGGATACCAGGCTTATATCGATGAGGAAGATCTAAAAAGAGGGGAAGAAATAAAAGAACTGTTTTGGGCAATCGAAGAGTCAAAGATCTCTATCATTGTCTTCTCAAAGAGGTATGCGGATTCGAGTTGGTGTCTTGATGAGCTGGTGAAGATCATGGAGTGTAGATCCAAATTGGGACAACGTGTTTTGCCAATATTCTATCATGTTGATCCTTCACATGTCAGGAATCAGAACGGAGATTTAGCTCAAGCATTTCAGAAGCACGAAGAGGGCATCCGTGAAGAAAAAGATGACAAGGAACGTGAAGCTAAACAAGAAAGGGTAAAGCAATGGAGAAAGGCTCTTGCAGAAGCTGCAAATTTGTCTGGCCACCATTTTCAAATCACAGACAATGGGCGCGAAGCAAAGGTTATTAGAGAAATTGTTGACAAGATTATTAAGGAATGGCTTTCGAGGGTAGACAAATTACATGTGGCCAAGCACCAAGTTGGAATCAATTCTCGCATTCAAAATATTATCACTTATCTTTCAAGTGGTGGATTAAATGATGTTGTCATGATTGGGATTTGGGGGATGGGTGGATTGGGTAAAACAACAGCTGCCAAAGCCATTTATAACCAAATTCATTCTAAGTTTGAATTCAAAAGTTTCCTGGCCGACGTTAGCAACAATACAAGTAAACATGGTCTGGTTAATTTGCAAGAAAAACTTATTTCTGACATCTTGAAAGAGAAGCCTCACATAAGCAGTGTTGATGAAGGTATCAGTATGATAAAAGATCTCCAACATAGACGGGTACTTGTCATCATAGACAATATAGATGAAGTGGAACAACTGGATGCAATAGCTGGAAAACATGATTGGTTTGGTCCAGGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

339

Amino Acids

38.44

Weight (kDa)

6.55

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 197 5.8e-48 TIR domain
TIR_2 PF13676 21 - 122 2.8e-16 TIR domain
NB-ARC PF00931 213 - 327 1.3e-10 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AccI GTMKAC 1 cut(s) 594
AccII CGCG 4 cut(s) 12, 57, 91, 534
AciI CCGC 3 cut(s) 10, 18, 251
AclWI GGATC 2 cut(s) 294, 338
AcoI YGGCCR 3 cut(s) 502, 609, 789
AcsI RAATTY 3 cut(s) 493, 756, 773
AcuI CTGAAG 1 cut(s) 58
AfaI GTAC 1 cut(s) 933
AflIII ACRYGT 4 cut(s) 60, 316, 352, 604
AgsI TTSAA 6 cut(s) 515, 644, 666, 773, 778, 862
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 3 cut(s) 141, 786, 1006
AjuI GAANNNNNNNTTGG 2 cut(s) 979, 1011
AluBI AGCT 7 cut(s) 72, 277, 380, 443, 488, 734, 983
AluI AGCT 7 cut(s) 72, 277, 380, 443, 488, 734, 983
AlwI GGATC 2 cut(s) 294, 338
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 4 cut(s) 106, 502, 609, 789
ApeKI GCWGC 3 cut(s) 119, 488, 734
ApoI RAATTY 3 cut(s) 493, 756, 773
Asp700I GAANNNNTTC 1 cut(s) 631
AspLEI GCGC 2 cut(s) 93, 534
AspS9I GGNCC 2 cut(s) 106, 1004
AsuHPI GGTGA 1 cut(s) 292
AvaII GGWCC 1 cut(s) 1004
BalI TGGCCA 2 cut(s) 504, 611
BbsI GAAGAC 2 cut(s) 90, 226
BbvI GCAGC 3 cut(s) 131, 475, 721
BccI CCATC 1 cut(s) 703
BciT130I CCWGG 3 cut(s) 143, 788, 1008
BciVI GTATCC 1 cut(s) 131
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 3 cut(s) 163, 219, 913
BisI GCNGC 4 cut(s) 18, 120, 489, 735
BlsI GCNGC 4 cut(s) 19, 121, 490, 736
Bme1390I CCNGG 3 cut(s) 143, 788, 1008
Bme18I GGWCC 1 cut(s) 1004
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 2 cut(s) 106, 1004
BmrFI CCNGG 3 cut(s) 143, 788, 1008
BmsI GCATC 2 cut(s) 417, 964
BpiI GAAGAC 2 cut(s) 90, 226
BplI GAGNNNNNCTC 2 cut(s) 460, 492
BpuEI CTTGAG 2 cut(s) 88, 366
Bsa29I ATCGAT 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 1007
Bse1I ACTGG 1 cut(s) 975
Bse3DI GCAATG 1 cut(s) 470
Bse8I GATNNNNATC 1 cut(s) 224
BseBI CCWGG 3 cut(s) 143, 788, 1008
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 1007
BseGI GGATG 4 cut(s) 26, 408, 714, 979
BseJI GATNNNNATC 1 cut(s) 224
BseMI GCAATG 1 cut(s) 470
BseNI ACTGG 1 cut(s) 975
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 3 cut(s) 131, 475, 721
Bsh1236I CGCG 4 cut(s) 12, 57, 91, 534
BshFI GGCC 4 cut(s) 108, 504, 611, 791
BshVI ATCGAT 1 cut(s) 153
BslFI GGGAC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 324
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 4 cut(s) 108, 504, 611, 791
Bsp143I GATC 6 cut(s) 163, 219, 285, 299, 343, 913
Bsp68I TCGCGA 1 cut(s) 57
BspACI CCGC 3 cut(s) 10, 18, 251
BspANI GGCC 4 cut(s) 108, 504, 611, 791
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 4 cut(s) 12, 57, 91, 534
BspHI TCATGA 1 cut(s) 690
BspPI GGATC 2 cut(s) 294, 338
BsrDI GCAATG 1 cut(s) 470
BsrI ACTGG 1 cut(s) 975
BssECI CCNNGG 1 cut(s) 1007
BssMI GATC 6 cut(s) 163, 219, 285, 299, 343, 913
Bst2UI CCWGG 3 cut(s) 143, 788, 1008
Bst4CI ACNGT 1 cut(s) 195
Bst6I CTCTTC 3 cut(s) 28, 204, 396
BstAPI GCANNNNNTGC 1 cut(s) 488
BstC8I GCNNGC 1 cut(s) 10
BstDEI CTNAG 1 cut(s) 765
BstF5I GGATG 4 cut(s) 26, 408, 714, 979
BstFNI CGCG 4 cut(s) 12, 57, 91, 534
BstHHI GCGC 2 cut(s) 93, 534
BstKTI GATC 6 cut(s) 166, 222, 288, 302, 346, 916
BstMBI GATC 6 cut(s) 163, 219, 285, 299, 343, 913
BstMWI GCNNNNNNNGC 5 cut(s) 14, 78, 105, 488, 740
BstNI CCWGG 3 cut(s) 143, 788, 1008
BstNSI RCATGY 2 cut(s) 356, 608
BstSCI CCNGG 3 cut(s) 141, 786, 1006
BstUI CGCG 4 cut(s) 12, 57, 91, 534
BstV1I GCAGC 3 cut(s) 131, 475, 721
BstV2I GAAGAC 2 cut(s) 90, 226
BstX2I RGATCY 4 cut(s) 163, 219, 299, 913
BstYI RGATCY 4 cut(s) 163, 219, 299, 913
Bsu15I ATCGAT 1 cut(s) 153
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 4 cut(s) 108, 504, 611, 791
BsuTUI ATCGAT 1 cut(s) 153
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 4 cut(s) 26, 408, 714, 979
BtsI GCAGTG 2 cut(s) 100, 889
BtsIMutI CAGTG 2 cut(s) 100, 889
BtuMI TCGCGA 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 10
CaiI CAGNNNCTG 1 cut(s) 488
CciI TCATGA 1 cut(s) 690
CfoI GCGC 2 cut(s) 93, 534
Cfr13I GGNCC 2 cut(s) 106, 1004
ClaI ATCGAT 1 cut(s) 153
Csp6I GTAC 1 cut(s) 932
CviAII CATG 8 cut(s) 37, 289, 338, 353, 605, 691, 818, 992
CviQI GTAC 1 cut(s) 932
DdeI CTNAG 1 cut(s) 765
DpnI GATC 6 cut(s) 165, 221, 287, 301, 345, 915
DpnII GATC 6 cut(s) 163, 219, 285, 299, 343, 913
EaeI YGGCCR 3 cut(s) 502, 609, 789
Eam1104I CTCTTC 3 cut(s) 28, 204, 396
EarI CTCTTC 3 cut(s) 28, 204, 396
Eco47I GGWCC 1 cut(s) 1004
Eco57I CTGAAG 1 cut(s) 58
EcoRI GAATTC 1 cut(s) 773
EcoRII CCWGG 3 cut(s) 141, 786, 1006
FaeI CATG 8 cut(s) 40, 292, 341, 356, 608, 694, 821, 995
FalI AAGNNNNNCTT 2 cut(s) 828, 860
FaqI GGGAC 1 cut(s) 324
FatI CATG 8 cut(s) 36, 288, 337, 352, 604, 690, 817, 991
FauI CCCGC 1 cut(s) 17
FblI GTMKAC 1 cut(s) 594
Fnu4HI GCNGC 4 cut(s) 18, 120, 489, 735
FokI GGATG 4 cut(s) 13, 395, 721, 986
Fsp4HI GCNGC 4 cut(s) 18, 120, 489, 735
GlaI GCGC 2 cut(s) 92, 533
GluI GCNGC 4 cut(s) 18, 120, 489, 735
HaeIII GGCC 4 cut(s) 108, 504, 611, 791
HhaI GCGC 2 cut(s) 93, 534
Hin1II CATG 8 cut(s) 40, 292, 341, 356, 608, 694, 821, 995
Hin6I GCGC 2 cut(s) 91, 532
HinP1I GCGC 2 cut(s) 91, 532
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 5 cut(s) 52, 212, 254, 361, 627
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 3 cut(s) 562, 595, 815
Hpy188I TCNGA 4 cut(s) 77, 366, 393, 853
Hpy188III TCNNGA 6 cut(s) 56, 67, 269, 358, 691, 859
Hpy8I GTNNAC 3 cut(s) 562, 595, 815
Hpy99I CGWCG 2 cut(s) 62, 797
HpyAV CCTTC 2 cut(s) 357, 887
HpyCH4III ACNGT 1 cut(s) 195
HpyCH4IV ACGT 4 cut(s) 60, 316, 436, 795
HpyCH4V TGCA 4 cut(s) 482, 491, 835, 977
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 78, 105, 488, 740
HpyF3I CTNAG 1 cut(s) 765
HpySE526I ACGT 4 cut(s) 60, 316, 436, 795
Hsp92II CATG 8 cut(s) 40, 292, 341, 356, 608, 694, 821, 995
HspAI GCGC 2 cut(s) 91, 532
Kzo9I GATC 6 cut(s) 163, 219, 285, 299, 343, 913
Lsp1109I GCAGC 3 cut(s) 131, 475, 721
LweI GCATC 2 cut(s) 417, 964
MaeII ACGT 4 cut(s) 60, 316, 436, 795
MalI GATC 6 cut(s) 165, 221, 287, 301, 345, 915
MboI GATC 6 cut(s) 163, 219, 285, 299, 343, 913
MboII GAAGA 9 cut(s) 15, 95, 173, 191, 221, 226, 295, 413, 428
MflI RGATCY 4 cut(s) 163, 219, 299, 913
MlsI TGGCCA 2 cut(s) 504, 611
MluCI AATT 8 cut(s) 305, 493, 555, 599, 631, 756, 773, 829
MluNI TGGCCA 2 cut(s) 504, 611
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 2 cut(s) 604, 943
Mox20I TGGCCA 2 cut(s) 504, 611
MroXI GAANNNNTTC 1 cut(s) 631
MscI TGGCCA 2 cut(s) 504, 611
MseI TTAA 4 cut(s) 125, 573, 677, 828
Msp20I TGGCCA 2 cut(s) 504, 611
MspA1I CMGCKG 1 cut(s) 734
MspR9I CCNGG 3 cut(s) 143, 788, 1008
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 3 cut(s) 143, 788, 1008
MvnI CGCG 4 cut(s) 12, 57, 91, 534
MwoI GCNNNNNNNGC 5 cut(s) 14, 78, 105, 488, 740
NdeII GATC 6 cut(s) 163, 219, 285, 299, 343, 913
NlaIII CATG 8 cut(s) 40, 292, 341, 356, 608, 694, 821, 995
NruI TCGCGA 1 cut(s) 57
NspI RCATGY 2 cut(s) 356, 608
PagI TCATGA 1 cut(s) 690
PciI ACATGT 2 cut(s) 352, 604
PctI GAATGC 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 631
PfeI GAWTC 4 cut(s) 52, 254, 361, 627
PkrI GCNGC 4 cut(s) 19, 121, 490, 736
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
PscI ACATGT 2 cut(s) 352, 604
PsiI TTATAA 1 cut(s) 750
Psp6I CCWGG 3 cut(s) 141, 786, 1006
PspGI CCWGG 3 cut(s) 141, 786, 1006
PspPI GGNCC 2 cut(s) 106, 1004
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 4 cut(s) 163, 219, 299, 913
PvuII CAGCTG 1 cut(s) 734
RruI TCGCGA 1 cut(s) 57
RsaI GTAC 1 cut(s) 933
RsaNI GTAC 1 cut(s) 932
SaqAI TTAA 4 cut(s) 125, 573, 677, 828
SatI GCNGC 4 cut(s) 18, 120, 489, 735
Sau3AI GATC 6 cut(s) 163, 219, 285, 299, 343, 913
Sau96I GGNCC 2 cut(s) 106, 1004
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 3 cut(s) 143, 788, 1008
SfaNI GCATC 2 cut(s) 417, 964
SinI GGWCC 1 cut(s) 1004
SmlI CTYRAG 2 cut(s) 67, 381
SmoI CTYRAG 2 cut(s) 67, 381
Sse9I AATT 8 cut(s) 305, 493, 555, 599, 631, 756, 773, 829
SsiI CCGC 3 cut(s) 10, 18, 251
SspI AATATT 2 cut(s) 330, 649
StyD4I CCNGG 3 cut(s) 141, 786, 1006
TaaI ACNGT 1 cut(s) 195
TaiI ACGT 4 cut(s) 63, 319, 439, 798
TaqI TCGA 4 cut(s) 153, 207, 257, 587
TasI AATT 8 cut(s) 305, 493, 555, 599, 631, 756, 773, 829
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 4 cut(s) 52, 254, 361, 627
Tru1I TTAA 4 cut(s) 125, 573, 677, 828
Tru9I TTAA 4 cut(s) 125, 573, 677, 828
TscAI CASTG 2 cut(s) 100, 889
TseI GCWGC 3 cut(s) 119, 488, 734
TspDTI ATGAA 4 cut(s) 15, 749, 906, 972
TspGWI ACGGA 2 cut(s) 384, 401
TspRI CASTG 2 cut(s) 100, 889
VpaK11BI GGWCC 1 cut(s) 1004
XapI RAATTY 3 cut(s) 493, 756, 773
XceI RCATGY 2 cut(s) 356, 608
XmiI GTMKAC 1 cut(s) 594
XmnI GAANNNNTTC 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.