MD02G1038400.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013733
Physical Location & Seq
Forward (+)
3146609 .. 3152747
6139 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1038400.v1.1.491

Sequence Viewer

Length: 843 bp
ATGACAGCCCACGAAGCCTCCTCTTCATCCTCCTCCAAGTCAAAACTTTGGAATTACGACGTGTTCTTGAGCTTCCGAGGCGAAGACACGCGCAAGGGCTTCACAGGCCACCTCCACGCGGCATTCAAAGACAGGGGATACCAGGCTTATATGGATGAGGACGATCTGAAAAGAGGGGAAGAAATAAAAGACGAACTGTTCCGGGCAATCGAAGAGTCGCGGATCTCTATCATTGTCTTCTCAAAGAGGTATGCGGATTCGAGTTGGTGTCTTGACGAGCTGGTGAAGATCATGGAGTGCAGATCCAAACTGGATCGACATGTTTTGCCAATATTCTATCATGTTGATCCTTCACATGTCAGGAAGCAGGACGGAGATTTAGCCGAAGCATTTCAGAAGCACAAAGAGGGCATCGGTGAAGAAAAAGATGGCAAGAAACGTGAAGCTAAACAAAAAAAGGTAGGGCAGTGGAGAGAGGCTCTTACAAAAGCTGCAAACTTGTCTGGCCACCACCTTCAAATCACTGACAATGGGCACGAAGCAAATCTTATTAGAGAAATTGTTGACAATAGTATTACGAAATGGCTTATGAGCACAAACAAACTAAATGTGGCCAAGCACCCAGTTGGTATCGATTCTCGCATTCAAGATATTATCAGTTATCTTTCAAGTGGCGGATCAAATGTTGTCATGGTTGGAGTTTGGGGGATGGGTGGATTGGGTAAAACAACAACTGCCAAAGCCATTTATAATCAAATTCATCATAAGTTCCAATTCAAAAGTTTCATGCCCGACATTAGCAACACTGCAAGTAAACATGTGTTGATGAAGGTATCGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

281

Amino Acids

31.72

Weight (kDa)

8.98

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 200 1.5e-50 TIR domain
TIR_2 PF13676 21 - 118 4.1e-18 TIR domain
NB-ARC PF00931 214 - 275 1.9e-06 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AccII CGCG 3 cut(s) 91, 119, 220
AciI CCGC 4 cut(s) 119, 220, 254, 675
AclWI GGATC 5 cut(s) 230, 297, 321, 341, 685
AcoI YGGCCR 2 cut(s) 505, 612
AcsI RAATTY 1 cut(s) 756
AfiI CCNNNNNNNGG 1 cut(s) 118
AflIII ACRYGT 4 cut(s) 60, 319, 355, 817
AgsI TTSAA 5 cut(s) 127, 518, 647, 669, 778
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 4 cut(s) 72, 280, 446, 491
AluI AGCT 4 cut(s) 72, 280, 446, 491
Alw21I GWGCWC 1 cut(s) 596
AlwI GGATC 5 cut(s) 230, 297, 321, 341, 685
AoxI GGCC 3 cut(s) 106, 505, 612
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 756
Asp700I GAANNNNTTC 1 cut(s) 390
AspLEI GCGC 1 cut(s) 93
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 2 cut(s) 295, 428
BaeGI GKGCMC 1 cut(s) 537
BalI TGGCCA 2 cut(s) 507, 614
BbsI GAAGAC 2 cut(s) 90, 229
Bbv12I GWGCWC 1 cut(s) 596
BbvI GCAGC 1 cut(s) 478
BccI CCATC 2 cut(s) 422, 703
BciT130I CCWGG 1 cut(s) 143
BciVI GTATCC 1 cut(s) 131
BcnI CCSGG 1 cut(s) 203
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 2 cut(s) 120, 492
BlsI GCNGC 2 cut(s) 121, 493
Bme1390I CCNGG 2 cut(s) 143, 203
BmgBI CACGTC 1 cut(s) 61
BmrFI CCNGG 2 cut(s) 143, 203
BmrI ACTGGG 1 cut(s) 617
BmsI GCATC 1 cut(s) 420
BmuI ACTGGG 1 cut(s) 617
BpiI GAAGAC 2 cut(s) 90, 229
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 203
Bsa29I ATCGAT 1 cut(s) 633
BsaBI GATNNNNATC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 76
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 2 cut(s) 315, 623
Bse8I GATNNNNATC 1 cut(s) 227
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 1 cut(s) 633
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 3 cut(s) 26, 160, 714
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 118
BseNI ACTGG 2 cut(s) 315, 623
BseRI GAGGAG 2 cut(s) 10, 22
BseSI GKGCMC 1 cut(s) 537
BseXI GCAGC 1 cut(s) 478
BsgI GTGCAG 1 cut(s) 319
Bsh1236I CGCG 3 cut(s) 91, 119, 220
BshFI GGCC 3 cut(s) 108, 507, 614
BshVI ATCGAT 1 cut(s) 633
BsiHKAI GWGCWC 1 cut(s) 596
BsiSI CCGG 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 118
BsmI GAATGC 2 cut(s) 122, 642
BsnI GGCC 3 cut(s) 108, 507, 614
Bsp1286I GDGCHC 2 cut(s) 537, 596
Bsp143I GATC 7 cut(s) 163, 222, 288, 302, 313, 346, 677
BspACI CCGC 4 cut(s) 119, 220, 254, 675
BspANI GGCC 3 cut(s) 108, 507, 614
BspDI ATCGAT 1 cut(s) 633
BspFNI CGCG 3 cut(s) 91, 119, 220
BspPI GGATC 5 cut(s) 230, 297, 321, 341, 685
BsrI ACTGG 2 cut(s) 315, 623
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 7 cut(s) 163, 222, 288, 302, 313, 346, 677
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 198
Bst6I CTCTTC 2 cut(s) 28, 207
BstF5I GGATG 3 cut(s) 26, 160, 714
BstFNI CGCG 3 cut(s) 91, 119, 220
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 7 cut(s) 166, 225, 291, 305, 316, 349, 680
BstMBI GATC 7 cut(s) 163, 222, 288, 302, 313, 346, 677
BstMWI GCNNNNNNNGC 3 cut(s) 14, 78, 105
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 3 cut(s) 323, 359, 821
BstSCI CCNGG 2 cut(s) 141, 201
BstSLI GKGCMC 1 cut(s) 537
BstUI CGCG 3 cut(s) 91, 119, 220
BstV1I GCAGC 1 cut(s) 478
BstV2I GAAGAC 2 cut(s) 90, 229
BstX2I RGATCY 2 cut(s) 222, 302
BstYI RGATCY 2 cut(s) 222, 302
Bsu15I ATCGAT 1 cut(s) 633
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 3 cut(s) 108, 507, 614
BsuTUI ATCGAT 1 cut(s) 633
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 3 cut(s) 26, 160, 714
BtsI GCAGTG 2 cut(s) 473, 804
BtsIMutI CAGTG 3 cut(s) 473, 522, 804
CfoI GCGC 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 633
CviAII CATG 7 cut(s) 292, 320, 341, 356, 691, 787, 818
DpnI GATC 7 cut(s) 165, 224, 290, 304, 315, 348, 679
DpnII GATC 7 cut(s) 163, 222, 288, 302, 313, 346, 677
EaeI YGGCCR 2 cut(s) 505, 612
Eam1104I CTCTTC 2 cut(s) 28, 207
EarI CTCTTC 2 cut(s) 28, 207
EciI GGCGGA 1 cut(s) 690
EcoRII CCWGG 1 cut(s) 141
FaeI CATG 7 cut(s) 295, 323, 344, 359, 694, 790, 821
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FatI CATG 7 cut(s) 291, 319, 340, 355, 690, 786, 817
Fnu4HI GCNGC 2 cut(s) 120, 492
FokI GGATG 3 cut(s) 13, 167, 721
Fsp4HI GCNGC 2 cut(s) 120, 492
GlaI GCGC 1 cut(s) 92
GluI GCNGC 2 cut(s) 120, 492
HaeIII GGCC 3 cut(s) 108, 507, 614
HapII CCGG 1 cut(s) 202
HhaI GCGC 1 cut(s) 93
Hin1II CATG 7 cut(s) 295, 323, 344, 359, 694, 790, 821
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 3 cut(s) 215, 257, 635
HpaII CCGG 1 cut(s) 202
HphI GGTGA 2 cut(s) 295, 428
Hpy166II GTNNAC 2 cut(s) 565, 815
Hpy188I TCNGA 4 cut(s) 77, 168, 396, 842
Hpy188III TCNNGA 4 cut(s) 67, 272, 361, 647
Hpy8I GTNNAC 2 cut(s) 565, 815
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 360, 524, 823
HpyCH4III ACNGT 1 cut(s) 198
HpyCH4IV ACGT 2 cut(s) 60, 439
HpyCH4V TGCA 3 cut(s) 300, 494, 809
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 78, 105
HpySE526I ACGT 2 cut(s) 60, 439
Hsp92II CATG 7 cut(s) 295, 323, 344, 359, 694, 790, 821
HspAI GCGC 1 cut(s) 91
Kzo9I GATC 7 cut(s) 163, 222, 288, 302, 313, 346, 677
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 1 cut(s) 420
MaeII ACGT 2 cut(s) 60, 439
MalI GATC 7 cut(s) 165, 224, 290, 304, 315, 348, 679
MboI GATC 7 cut(s) 163, 222, 288, 302, 313, 346, 677
MboII GAAGA 7 cut(s) 15, 95, 191, 224, 229, 298, 431
MflI RGATCY 2 cut(s) 222, 302
MhlI GDGCHC 2 cut(s) 537, 596
MlsI TGGCCA 2 cut(s) 507, 614
MluCI AATT 4 cut(s) 52, 558, 756, 773
MluNI TGGCCA 2 cut(s) 507, 614
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 676
Mox20I TGGCCA 2 cut(s) 507, 614
MroXI GAANNNNTTC 1 cut(s) 390
MscI TGGCCA 2 cut(s) 507, 614
Msp20I TGGCCA 2 cut(s) 507, 614
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 2 cut(s) 143, 203
Mva1269I GAATGC 2 cut(s) 122, 642
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 3 cut(s) 91, 119, 220
MwoI GCNNNNNNNGC 3 cut(s) 14, 78, 105
NciI CCSGG 1 cut(s) 203
NdeII GATC 7 cut(s) 163, 222, 288, 302, 313, 346, 677
NlaIII CATG 7 cut(s) 295, 323, 344, 359, 694, 790, 821
NspI RCATGY 3 cut(s) 323, 359, 821
PciI ACATGT 3 cut(s) 319, 355, 817
PctI GAATGC 2 cut(s) 122, 642
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 2 cut(s) 257, 635
PkrI GCNGC 2 cut(s) 121, 493
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PscI ACATGT 3 cut(s) 319, 355, 817
PsiI TTATAA 1 cut(s) 750
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PsuI RGATCY 2 cut(s) 222, 302
SatI GCNGC 2 cut(s) 120, 492
Sau3AI GATC 7 cut(s) 163, 222, 288, 302, 313, 346, 677
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 143, 203
SduI GDGCHC 2 cut(s) 537, 596
SfaNI GCATC 1 cut(s) 420
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 4 cut(s) 52, 558, 756, 773
SsiI CCGC 4 cut(s) 119, 220, 254, 675
SspI AATATT 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 141, 201
TaaI ACNGT 1 cut(s) 198
TaiI ACGT 2 cut(s) 63, 442
TaqI TCGA 4 cut(s) 210, 260, 316, 633
TaqII GACCGA 1 cut(s) 826
TasI AATT 4 cut(s) 52, 558, 756, 773
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 2 cut(s) 257, 635
TscAI CASTG 3 cut(s) 473, 529, 811
TseI GCWGC 1 cut(s) 491
TspDTI ATGAA 4 cut(s) 15, 749, 775, 842
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 3 cut(s) 473, 529, 811
XapI RAATTY 1 cut(s) 756
XceI RCATGY 3 cut(s) 323, 359, 821
XmnI GAANNNNTTC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.