pycom02g03460

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013733
Physical Location & Seq
Reverse (-)
2290739 .. 2292097
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g03460.2

Sequence Viewer

Length: 666 bp
ATGAGGCCACGTTGTTGGAATTTTGTACCCATGTTCAATGTTTTTCTCTTTTATTTATATGTGAGTATCATCCTTCGGCAACACAAATACGACGACTGCAGGGCCATAATGGTGGTTACCGCCATGACAGCTGCCTCCTCTTCATCCTACTCCAAGTCAAAACTTTGGAAGTACGATGTGTTCTTGAGCTTCAGCGGCAAAGACACGCGCAATGGCTTCACGAGCCACCTCCACGCGACATTGAAAGACAGAGGATACGAGGTCTTTATTGATGAGGACGATCTAAAAAGAGGGGAAGAAATAAAAGAGGAACTGTTTCGGGCAATCAAAGCGTCGAGAATCTCTGTCATTGTCTTCTCAAAGACGTATGCGGATTCGAGTTGGTGTCTTGACGAGCTGGTGAACATCATGGAGTGCAGAGACAAACTGGGGCGACATGTTTTGCCAATATTCTATCACGTCGATCCTTCGCACGTCAGGAAGCAGGACGGTGATTTAGCCGAAGCATTTCAGAAGCACGAAAAGGCCATCCGTGAAGAAAAAGATGACAAGAAACGTGAAGCTAAACAAGAAAGGGTAAAGCAATGGAGAGAGGCTCTCACAAAAGCTGCAAATTTGTCTGGCCACCCTCTTAAAATCATTAACTATAGGTCAACATTCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

222

Amino Acids

25.96

Weight (kDa)

9.14

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 208, 236
AciI CCGC 3 cut(s) 120, 195, 371
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 622
AcsI RAATTY 2 cut(s) 19, 613
AcuI CTGAAG 1 cut(s) 175
AfaI GTAC 2 cut(s) 27, 173
AflIII ACRYGT 1 cut(s) 436
AgsI TTSAA 2 cut(s) 37, 244
AjiI CACGTC 2 cut(s) 460, 475
AluBI AGCT 6 cut(s) 131, 189, 397, 563, 608, 663
AluI AGCT 6 cut(s) 131, 189, 397, 563, 608, 663
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 1 cut(s) 458
AoxI GGCC 4 cut(s) 5, 102, 525, 622
ApeKI GCWGC 2 cut(s) 131, 608
ApoI RAATTY 2 cut(s) 19, 613
Asp700I GAANNNNTTC 2 cut(s) 315, 507
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 102
AsuHPI GGTGA 2 cut(s) 412, 503
BalI TGGCCA 1 cut(s) 624
BauI CACGAG 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 118, 595
BccI CCATC 1 cut(s) 536
BciVI GTATCC 1 cut(s) 248
BcoDI GTCTC 1 cut(s) 414
BfmI CTRYAG 2 cut(s) 97, 646
BfuI GTATCC 1 cut(s) 248
BisI GCNGC 3 cut(s) 132, 196, 609
BlsI GCNGC 3 cut(s) 133, 197, 610
BmgBI CACGTC 2 cut(s) 460, 475
BmgT120I GGNCC 1 cut(s) 102
BmrI ACTGGG 1 cut(s) 437
BmuI ACTGGG 1 cut(s) 437
BpiI GAAGAC 1 cut(s) 346
BplI GAGNNNNNCTC 4 cut(s) 580, 612, 582, 614
BpuEI CTTGAG 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 432
Bse3DI GCAATG 2 cut(s) 217, 590
BseGI GGATG 3 cut(s) 69, 143, 528
BseMI GCAATG 2 cut(s) 217, 590
BseNI ACTGG 1 cut(s) 432
BseRI GAGGAG 1 cut(s) 127
BseXI GCAGC 2 cut(s) 118, 595
BsgI GTGCAG 1 cut(s) 436
Bsh1236I CGCG 2 cut(s) 208, 236
BshFI GGCC 4 cut(s) 7, 104, 527, 624
BsmAI GTCTC 1 cut(s) 414
BsnI GGCC 4 cut(s) 7, 104, 527, 624
Bsp143I GATC 2 cut(s) 280, 463
BspACI CCGC 3 cut(s) 120, 195, 371
BspANI GGCC 4 cut(s) 7, 104, 527, 624
BspFNI CGCG 2 cut(s) 208, 236
BspMAI CTGCAG 1 cut(s) 101
BspPI GGATC 1 cut(s) 458
BsrDI GCAATG 2 cut(s) 217, 590
BsrI ACTGG 1 cut(s) 432
BssMI GATC 2 cut(s) 280, 463
BssSI CACGAG 1 cut(s) 220
Bst2BI CACGAG 1 cut(s) 220
Bst4CI ACNGT 2 cut(s) 315, 491
Bst6I CTCTTC 1 cut(s) 145
BstEII GGTNACC 1 cut(s) 115
BstF5I GGATG 3 cut(s) 69, 143, 528
BstFNI CGCG 2 cut(s) 208, 236
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 2 cut(s) 283, 466
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 2 cut(s) 280, 463
BstMWI GCNNNNNNNGC 4 cut(s) 128, 195, 222, 329
BstNSI RCATGY 1 cut(s) 440
BstPI GGTNACC 1 cut(s) 115
BstSFI CTRYAG 2 cut(s) 97, 646
BstUI CGCG 2 cut(s) 208, 236
BstV1I GCAGC 2 cut(s) 118, 595
BstV2I GAAGAC 1 cut(s) 346
BstXI CCANNNNNNTGG 2 cut(s) 15, 112
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 4 cut(s) 7, 104, 527, 624
BtrI CACGTC 2 cut(s) 460, 475
BtsCI GGATG 3 cut(s) 69, 143, 528
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 102
CseI GACGC 1 cut(s) 321
Csp6I GTAC 2 cut(s) 26, 172
CviAII CATG 4 cut(s) 31, 124, 409, 437
CviQI GTAC 2 cut(s) 26, 172
DpnI GATC 2 cut(s) 282, 465
DpnII GATC 2 cut(s) 280, 463
EaeI YGGCCR 1 cut(s) 622
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 175
Eco91I GGTNACC 1 cut(s) 115
EcoO65I GGTNACC 1 cut(s) 115
FaeI CATG 4 cut(s) 34, 127, 412, 440
FaiI YATR 9 cut(s) 32, 58, 60, 107, 125, 369, 410, 438, 648
FatI CATG 4 cut(s) 30, 123, 408, 436
Fnu4HI GCNGC 3 cut(s) 132, 196, 609
FokI GGATG 3 cut(s) 56, 130, 515
Fsp4HI GCNGC 3 cut(s) 132, 196, 609
GlaI GCGC 1 cut(s) 209
GluI GCNGC 3 cut(s) 132, 196, 609
HaeIII GGCC 4 cut(s) 7, 104, 527, 624
HgaI GACGC 1 cut(s) 321
HhaI GCGC 1 cut(s) 210
Hin1II CATG 4 cut(s) 34, 127, 412, 440
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 654
HindII GTYRAC 1 cut(s) 654
HinfI GANTC 2 cut(s) 339, 374
HphI GGTGA 2 cut(s) 412, 503
Hpy166II GTNNAC 2 cut(s) 403, 654
Hpy188I TCNGA 1 cut(s) 513
Hpy188III TCNNGA 5 cut(s) 184, 220, 336, 389, 478
Hpy8I GTNNAC 2 cut(s) 403, 654
Hpy99I CGWCG 3 cut(s) 95, 337, 464
HpyAV CCTTC 2 cut(s) 83, 477
HpyCH4III ACNGT 2 cut(s) 315, 491
HpyCH4IV ACGT 5 cut(s) 10, 365, 459, 474, 556
HpyCH4V TGCA 3 cut(s) 99, 417, 611
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 195, 222, 329
HpySE526I ACGT 5 cut(s) 10, 365, 459, 474, 556
Hsp92II CATG 4 cut(s) 34, 127, 412, 440
HspAI GCGC 1 cut(s) 208
Kzo9I GATC 2 cut(s) 280, 463
LpnPI CCDG 6 cut(s) 85, 383, 413, 463, 470, 606
Lsp1109I GCAGC 2 cut(s) 118, 595
MaeII ACGT 5 cut(s) 10, 365, 459, 474, 556
MaeIII GTNAC 1 cut(s) 115
MalI GATC 2 cut(s) 282, 465
MboI GATC 2 cut(s) 280, 463
MboII GAAGA 4 cut(s) 132, 308, 346, 548
MlsI TGGCCA 1 cut(s) 624
MluCI AATT 2 cut(s) 19, 613
MluNI TGGCCA 1 cut(s) 624
Mox20I TGGCCA 1 cut(s) 624
MroXI GAANNNNTTC 2 cut(s) 315, 507
MscI TGGCCA 1 cut(s) 624
MseI TTAA 2 cut(s) 633, 642
MslI CAYNNNNRTG 1 cut(s) 110
Msp20I TGGCCA 1 cut(s) 624
MspA1I CMGCKG 3 cut(s) 131, 195, 663
MvnI CGCG 2 cut(s) 208, 236
MwoI GCNNNNNNNGC 4 cut(s) 128, 195, 222, 329
NdeII GATC 2 cut(s) 280, 463
NlaIII CATG 4 cut(s) 34, 127, 412, 440
NspI RCATGY 1 cut(s) 440
PciI ACATGT 1 cut(s) 436
PdmI GAANNNNTTC 2 cut(s) 315, 507
PfeI GAWTC 2 cut(s) 339, 374
PkrI GCNGC 3 cut(s) 133, 197, 610
PscI ACATGT 1 cut(s) 436
PspEI GGTNACC 1 cut(s) 115
PspPI GGNCC 1 cut(s) 102
PsrI GAACNNNNNNTAC 2 cut(s) 164, 196
PstI CTGCAG 1 cut(s) 101
PvuII CAGCTG 2 cut(s) 131, 663
RsaI GTAC 2 cut(s) 27, 173
RsaNI GTAC 2 cut(s) 26, 172
RseI CAYNNNNRTG 1 cut(s) 110
SaqAI TTAA 2 cut(s) 633, 642
SatI GCNGC 3 cut(s) 132, 196, 609
Sau3AI GATC 2 cut(s) 280, 463
Sau96I GGNCC 1 cut(s) 102
SfcI CTRYAG 2 cut(s) 97, 646
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
Sse9I AATT 2 cut(s) 19, 613
SsiI CCGC 3 cut(s) 120, 195, 371
SspI AATATT 1 cut(s) 450
TaaI ACNGT 2 cut(s) 315, 491
TaiI ACGT 5 cut(s) 13, 368, 462, 477, 559
TaqI TCGA 3 cut(s) 335, 377, 462
TasI AATT 2 cut(s) 19, 613
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 2 cut(s) 339, 374
Tru1I TTAA 2 cut(s) 633, 642
Tru9I TTAA 2 cut(s) 633, 642
TseI GCWGC 2 cut(s) 131, 608
TspDTI ATGAA 1 cut(s) 132
TspGWI ACGGA 1 cut(s) 521
XapI RAATTY 2 cut(s) 19, 613
XceI RCATGY 1 cut(s) 440
XmnI GAANNNNTTC 2 cut(s) 315, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.