MD02G1050400.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013733
Physical Location & Seq
Reverse (-)
4165147 .. 4168730
3584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1050400.v1.1.491

Sequence Viewer

Length: 624 bp
ATGACGACCCACGAAGCCTCCGCTTCATCCTCCTCCAAGTCAAAACTTTGGAATTACGATGTGTTCTTGAGCTTCAGCGGTGTAGACACACGCAATGGCTTCACGGGCCACCTCCACAGGGCATTAACAGACAGGGGATACCAGGCTTATATTGATGAGGACAATCTAAAAAGAGGGGAAGAAATACAAAAAGAACTGTTCCAGGCAATCGAAGAATCGAAGATCTTTATCATTGTATTCTCATCGATGTATGCTAATTCGAGTTGGTGTCTTAACGAGCTGGTGAAGATCATGGAGTGCAGAGACAAACCGGGGCGACATGTTTTGCCAATATTCTATCACGTTGATCCTTCACATGTCAGGAAGCAGAATGGAGTTTTAGCCCAAGCATTTAAGAAGCACAAAAAGAACAACCGTAAAGAAAAAGATGACAAGAAACGTGAAGAAAAACAAGAAAGGGTAAAGCAGTGGAGAAATGCTCTTAGAGAAGCTGCAGATTTGTCTGGCTACCATCTTCAAATCACTGATAATAGTGCTACAATGGTGTGGCTGGGGTTTGGAGGAGGCGCGAAGCACAGCTTATTAGAGAAGTTGTTGACCAGATTATTCCGAAATGGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

208

Amino Acids

23.96

Weight (kDa)

9.43

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 182 6.9e-48 TIR domain
TIR_2 PF13676 21 - 118 1.4e-18 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 84
AccII CGCG 1 cut(s) 569
AciI CCGC 2 cut(s) 21, 78
AclWI GGATC 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 118
AflIII ACRYGT 2 cut(s) 319, 355
AgsI TTSAA 1 cut(s) 518
AjnI CCWGG 2 cut(s) 141, 201
AluBI AGCT 4 cut(s) 72, 280, 491, 579
AluI AGCT 4 cut(s) 72, 280, 491, 579
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 1 cut(s) 341
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 491
AspLEI GCGC 1 cut(s) 569
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 295
BbvI GCAGC 1 cut(s) 478
BccI CCATC 1 cut(s) 519
BciT130I CCWGG 2 cut(s) 143, 203
BciVI GTATCC 1 cut(s) 131
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 1 cut(s) 297
BfmI CTRYAG 1 cut(s) 492
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 1 cut(s) 222
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
Bme1390I CCNGG 3 cut(s) 143, 203, 312
BmgT120I GGNCC 1 cut(s) 106
BmrFI CCNGG 3 cut(s) 143, 203, 312
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 311
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse3DI GCAATG 1 cut(s) 100
Bse8I GATNNNNATC 1 cut(s) 227
BseBI CCWGG 2 cut(s) 143, 203
BseCI ATCGAT 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 311
BseGI GGATG 1 cut(s) 26
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 100
BseRI GAGGAG 2 cut(s) 22, 576
BseXI GCAGC 1 cut(s) 478
BseYI CCCAGC 1 cut(s) 550
BsgI GTGCAG 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 569
BshFI GGCC 1 cut(s) 108
BshVI ATCGAT 1 cut(s) 245
BsiSI CCGG 1 cut(s) 311
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 297
BsnI GGCC 1 cut(s) 108
Bsp143I GATC 3 cut(s) 222, 288, 346
BspACI CCGC 2 cut(s) 21, 78
BspANI GGCC 1 cut(s) 108
BspDI ATCGAT 1 cut(s) 245
BspFNI CGCG 1 cut(s) 569
BspMAI CTGCAG 1 cut(s) 496
BspPI GGATC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 100
BssECI CCNNGG 1 cut(s) 311
BssMI GATC 3 cut(s) 222, 288, 346
Bst2UI CCWGG 2 cut(s) 143, 203
Bst4CI ACNGT 2 cut(s) 198, 416
BstDEI CTNAG 1 cut(s) 482
BstF5I GGATG 1 cut(s) 26
BstFNI CGCG 1 cut(s) 569
BstHHI GCGC 1 cut(s) 569
BstKTI GATC 3 cut(s) 225, 291, 349
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 3 cut(s) 222, 288, 346
BstMWI GCNNNNNNNGC 1 cut(s) 105
BstNI CCWGG 2 cut(s) 143, 203
BstNSI RCATGY 2 cut(s) 323, 359
BstSCI CCNGG 3 cut(s) 141, 201, 310
BstSFI CTRYAG 1 cut(s) 492
BstUI CGCG 1 cut(s) 569
BstV1I GCAGC 1 cut(s) 478
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 245
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 108
BsuTUI ATCGAT 1 cut(s) 245
BtsCI GGATG 1 cut(s) 26
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 2 cut(s) 473, 522
CfoI GCGC 1 cut(s) 569
Cfr13I GGNCC 1 cut(s) 106
ClaI ATCGAT 1 cut(s) 245
CviAII CATG 3 cut(s) 292, 320, 356
DdeI CTNAG 1 cut(s) 482
DpnI GATC 3 cut(s) 224, 290, 348
DpnII GATC 3 cut(s) 222, 288, 346
Eco57I CTGAAG 1 cut(s) 58
EcoRII CCWGG 2 cut(s) 141, 201
FaeI CATG 3 cut(s) 295, 323, 359
FaiI YATR 5 cut(s) 150, 252, 293, 321, 357
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FatI CATG 3 cut(s) 291, 319, 355
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 1 cut(s) 13
Fsp4HI GCNGC 1 cut(s) 492
GlaI GCGC 1 cut(s) 568
GluI GCNGC 1 cut(s) 492
GsaI CCCAGC 1 cut(s) 554
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 311
HhaI GCGC 1 cut(s) 569
Hin1II CATG 3 cut(s) 295, 323, 359
Hin6I GCGC 1 cut(s) 567
HinP1I GCGC 1 cut(s) 567
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HinfI GANTC 1 cut(s) 215
HpaII CCGG 1 cut(s) 311
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 85, 597
Hpy188I TCNGA 2 cut(s) 611, 623
Hpy188III TCNNGA 2 cut(s) 67, 361
Hpy8I GTNNAC 2 cut(s) 85, 597
HpyAV CCTTC 1 cut(s) 360
HpyCH4III ACNGT 2 cut(s) 198, 416
HpyCH4IV ACGT 2 cut(s) 342, 439
HpyCH4V TGCA 2 cut(s) 300, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 105
HpyF3I CTNAG 1 cut(s) 482
HpySE526I ACGT 2 cut(s) 342, 439
Hsp92II CATG 3 cut(s) 295, 323, 359
HspAI GCGC 1 cut(s) 567
Kzo9I GATC 3 cut(s) 222, 288, 346
Lsp1109I GCAGC 1 cut(s) 478
MaeII ACGT 2 cut(s) 342, 439
MalI GATC 3 cut(s) 224, 290, 348
MboI GATC 3 cut(s) 222, 288, 346
MboII GAAGA 6 cut(s) 191, 224, 232, 298, 455, 506
MflI RGATCY 1 cut(s) 222
MluCI AATT 2 cut(s) 52, 256
MnlI CCTC 8 cut(s) 28, 40, 43, 122, 151, 167, 554, 557
MseI TTAA 3 cut(s) 125, 273, 393
MspA1I CMGCKG 1 cut(s) 78
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 3 cut(s) 143, 203, 312
MvaI CCWGG 2 cut(s) 143, 203
MvnI CGCG 1 cut(s) 569
MwoI GCNNNNNNNGC 1 cut(s) 105
NciI CCSGG 1 cut(s) 312
NdeII GATC 3 cut(s) 222, 288, 346
NlaIII CATG 3 cut(s) 295, 323, 359
NspI RCATGY 2 cut(s) 323, 359
PciI ACATGT 2 cut(s) 319, 355
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 1 cut(s) 493
PscI ACATGT 2 cut(s) 319, 355
Psp6I CCWGG 2 cut(s) 141, 201
PspFI CCCAGC 1 cut(s) 550
PspGI CCWGG 2 cut(s) 141, 201
PspPI GGNCC 1 cut(s) 106
PstI CTGCAG 1 cut(s) 496
PsuI RGATCY 1 cut(s) 222
SaqAI TTAA 3 cut(s) 125, 273, 393
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 3 cut(s) 222, 288, 346
Sau96I GGNCC 1 cut(s) 106
ScrFI CCNGG 3 cut(s) 143, 203, 312
SetI ASST 7 cut(s) 74, 114, 282, 345, 442, 493, 581
SfcI CTRYAG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 2 cut(s) 52, 256
SsiI CCGC 2 cut(s) 21, 78
SspI AATATT 1 cut(s) 333
StyD4I CCNGG 3 cut(s) 141, 201, 310
TaaI ACNGT 2 cut(s) 198, 416
TaiI ACGT 2 cut(s) 345, 442
TaqI TCGA 4 cut(s) 210, 218, 245, 260
TasI AATT 2 cut(s) 52, 256
TfiI GAWTC 1 cut(s) 215
Tru1I TTAA 3 cut(s) 125, 273, 393
Tru9I TTAA 3 cut(s) 125, 273, 393
TscAI CASTG 2 cut(s) 473, 529
TseI GCWGC 1 cut(s) 491
TspDTI ATGAA 1 cut(s) 15
TspRI CASTG 2 cut(s) 473, 529
XceI RCATGY 2 cut(s) 323, 359
XmiI GTMKAC 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.