pycom02g04500

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013733
Physical Location & Seq
Reverse (-)
2927357 .. 2927704
348 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g04500.1

Sequence Viewer

Length: 348 bp
ATGAGAACCCACGAAGCCTCCTCTTCATCCTCCTCCATTTCAAAACTTTGGAATTACGACGTGTTCTTGAGCTTCAGTGGTGTAGACACACGCAATGGCTTCACGGGCCACCTCCACGCGGCATTAACAGACAGGGGATACCAGGCTTATATCGATGAGGATGATCTAGAAAGAGGGGAAGAAATAAAAGAGGAACTGTTCCGGGCAATCGAAGAGGCGAGGATCTCTATCATTGTCTTCTCAAAGAGTTATGCGGACTCGAGTTGGTGTCTTGACGAGCTGGTGATAGATCATGAAGTGCAGATACAAACTGGGGCGACATGTTTTGCCAATTTTCTATCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

116

Amino Acids

12.99

Weight (kDa)

4.55

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 101 4.6e-27 TIR domain
TIR_2 PF13676 21 - 95 1.6e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 84
AccII CGCG 1 cut(s) 119
AciI CCGC 2 cut(s) 119, 254
AclWI GGATC 1 cut(s) 230
AcuI CTGAAG 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 118
AflIII ACRYGT 2 cut(s) 60, 320
AgsI TTSAA 1 cut(s) 42
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 2 cut(s) 72, 280
AluI AGCT 2 cut(s) 72, 280
AlwI GGATC 1 cut(s) 230
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 1 cut(s) 106
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 295
AvaI CYCGRG 1 cut(s) 259
BarI GAAGNNNNNNTAC 2 cut(s) 288, 320
BbsI GAAGAC 1 cut(s) 229
BciT130I CCWGG 1 cut(s) 143
BciVI GTATCC 1 cut(s) 131
BcnI CCSGG 1 cut(s) 203
BfaI CTAG 1 cut(s) 167
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
Bme1390I CCNGG 2 cut(s) 143, 203
BmeT110I CYCGRG 1 cut(s) 259
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 106
BmrFI CCNGG 2 cut(s) 143, 203
BmrI ACTGGG 1 cut(s) 321
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 203
Bsa29I ATCGAT 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 227
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 100
Bse8I GATNNNNATC 1 cut(s) 227
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 1 cut(s) 153
BseGI GGATG 2 cut(s) 26, 166
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 100
BseNI ACTGG 1 cut(s) 316
BseRI GAGGAG 2 cut(s) 10, 22
BsgI GTGCAG 1 cut(s) 320
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 1 cut(s) 108
BshVI ATCGAT 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 118
BsnI GGCC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 259
Bsp143I GATC 3 cut(s) 163, 222, 289
BspACI CCGC 2 cut(s) 119, 254
BspANI GGCC 1 cut(s) 108
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 1 cut(s) 119
BspHI TCATGA 1 cut(s) 292
BspPI GGATC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 100
BsrI ACTGG 1 cut(s) 316
BssMI GATC 3 cut(s) 163, 222, 289
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 198
Bst6I CTCTTC 2 cut(s) 28, 207
BstF5I GGATG 2 cut(s) 26, 166
BstFNI CGCG 1 cut(s) 119
BstKTI GATC 3 cut(s) 166, 225, 292
BstMBI GATC 3 cut(s) 163, 222, 289
BstMWI GCNNNNNNNGC 1 cut(s) 105
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 1 cut(s) 324
BstSCI CCNGG 2 cut(s) 141, 201
BstUI CGCG 1 cut(s) 119
BstV2I GAAGAC 1 cut(s) 229
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 153
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 108
BsuTUI ATCGAT 1 cut(s) 153
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 26, 166
BtsIMutI CAGTG 1 cut(s) 82
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 106
ClaI ATCGAT 1 cut(s) 153
CviAII CATG 3 cut(s) 293, 321, 342
CviJI RGCY 6 cut(s) 17, 72, 99, 108, 146, 280
CviKI_1 RGCY 6 cut(s) 17, 72, 99, 108, 146, 280
DpnI GATC 3 cut(s) 165, 224, 291
DpnII GATC 3 cut(s) 163, 222, 289
Eam1104I CTCTTC 2 cut(s) 28, 207
EarI CTCTTC 2 cut(s) 28, 207
Eco57I CTGAAG 1 cut(s) 58
Eco88I CYCGRG 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 141
FaeI CATG 3 cut(s) 296, 324, 345
FaiI YATR 5 cut(s) 150, 252, 294, 322, 343
FatI CATG 3 cut(s) 292, 320, 341
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 2 cut(s) 13, 173
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 1 cut(s) 167
GluI GCNGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 202
Hin1II CATG 3 cut(s) 296, 324, 345
HinfI GANTC 1 cut(s) 257
HpaII CCGG 1 cut(s) 202
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 1 cut(s) 85
Hpy188III TCNNGA 4 cut(s) 67, 167, 272, 293
Hpy8I GTNNAC 1 cut(s) 85
Hpy99I CGWCG 1 cut(s) 62
HpyCH4III ACNGT 1 cut(s) 198
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 1 cut(s) 301
HpyF10VI GCNNNNNNNGC 1 cut(s) 105
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 3 cut(s) 296, 324, 345
Kzo9I GATC 3 cut(s) 163, 222, 289
LpnPI CCDG 6 cut(s) 118, 128, 155, 215, 266, 297
MaeI CTAG 1 cut(s) 167
MaeII ACGT 1 cut(s) 60
MalI GATC 3 cut(s) 165, 224, 291
MboI GATC 3 cut(s) 163, 222, 289
MboII GAAGA 4 cut(s) 15, 191, 224, 229
MflI RGATCY 1 cut(s) 222
MluCI AATT 2 cut(s) 52, 331
MlyI GAGTC 1 cut(s) 251
MseI TTAA 1 cut(s) 125
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 2 cut(s) 143, 203
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 105
NciI CCSGG 1 cut(s) 203
NdeII GATC 3 cut(s) 163, 222, 289
NlaIII CATG 3 cut(s) 296, 324, 345
NspI RCATGY 1 cut(s) 324
PaeR7I CTCGAG 1 cut(s) 259
PagI TCATGA 1 cut(s) 292
PciI ACATGT 1 cut(s) 320
PkrI GCNGC 1 cut(s) 121
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PscI ACATGT 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspPI GGNCC 1 cut(s) 106
PspXI VCTCGAGB 1 cut(s) 259
PsuI RGATCY 1 cut(s) 222
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 3 cut(s) 163, 222, 289
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 2 cut(s) 143, 203
SetI ASST 4 cut(s) 63, 74, 114, 282
Sfr274I CTCGAG 1 cut(s) 259
SlaI CTCGAG 1 cut(s) 259
SmlI CTYRAG 2 cut(s) 67, 259
SmoI CTYRAG 2 cut(s) 67, 259
Sse9I AATT 2 cut(s) 52, 331
SsiI CCGC 2 cut(s) 119, 254
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 2 cut(s) 141, 201
TaaI ACNGT 1 cut(s) 198
TaiI ACGT 1 cut(s) 63
TaqI TCGA 3 cut(s) 153, 210, 260
TasI AATT 2 cut(s) 52, 331
TauI GCSGC 1 cut(s) 122
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 82
TspDTI ATGAA 2 cut(s) 15, 309
TspRI CASTG 1 cut(s) 82
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 1 cut(s) 324
XhoI CTCGAG 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 84
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.