MD02G1023600.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013733
Physical Location & Seq
Reverse (-)
1742728 .. 1744003
1276 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1023600.v1.1.491

Sequence Viewer

Length: 831 bp
ATGTATGCGAATTCGAGTTGGTGTCTTGACGAGCTGGTGAAGATCATGGCGTGTAGAGACAAACTGGGGCGACATGTTTTACCAATATTCTATCACGTTGATCCTTCGCATGTCAGGAAGCAGGACGGAGATTTAGCCGAAGCATTTCAGAAGCACAAAAAGGACATCCGTGAAGAAAAAGATGACAAGAAACGTGAAGCTAAACAAGAAAGGGTAAAGCAGTGGAGAGAGGCTCTCACAAAGGCTGCAAATTTGTCTGGCCACCATCATAAAATCGCTAACAATGGGGGCGAAGCAGAGTTCATTAAAAAAATTATTAACGATAATATTTGTGAATGGCTCACCAGCACTAACGAATTACATGTGGCCAAGCACATAGTTGGAATCAATTCACGCGTTCAAGATATTATCAGTTATCTTTCAAGTGGTGGATCAAATGATGTTCTCATGGTTGGAATTTGGGGGATGGGTGGATTGGGTAAAACAACAGCTGCCAAGGCCATTTATAACCAAATTCATCCTCAGTTTGAATTCAAAAGTTTCCTTGCCGACGTTCGCGATGCTACAAGTAAACATGATCTGGTTGATGTGCAAAAGAAACTTATTTCTGACATCTTGAAAAAGAAGCGTGAAATAAGTTGTGTTGACGATGGTATCGACCTGATAAAACAACAATTTCGACATAGAAAGATACTTGTCATCGTGGACAACGTAGATAAAAAGGAACAACTGGATGCAATAGTTGGAAGTCGTCATTGGTTTGGTCCTGGAAGTAGAATTATCATAACGACACGAAATGAACGTCTACTACTAAATTTGGCATGCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

277

Amino Acids

31.46

Weight (kDa)

9.15

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 119 8.5e-25 TIR domain
NB-ARC PF00931 132 - 269 8.8e-16 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 507
AccI GTMKAC 1 cut(s) 805
AccII CGCG 2 cut(s) 396, 558
AclWI GGATC 2 cut(s) 95, 439
AcoI YGGCCR 2 cut(s) 259, 366
AcsI RAATTY 6 cut(s) 10, 250, 456, 513, 530, 814
AflIII ACRYGT 3 cut(s) 73, 361, 394
AgsI TTSAA 5 cut(s) 401, 423, 530, 535, 619
AjnI CCWGG 1 cut(s) 766
AjuI GAANNNNNNNTTGG 2 cut(s) 739, 771
AluBI AGCT 3 cut(s) 34, 200, 491
AluI AGCT 3 cut(s) 34, 200, 491
Alw26I GTCTC 1 cut(s) 51
AlwI GGATC 2 cut(s) 95, 439
AoxI GGCC 3 cut(s) 259, 366, 498
ApeKI GCWGC 2 cut(s) 245, 491
ApoI RAATTY 6 cut(s) 10, 250, 456, 513, 530, 814
ArsI GACNNNNNNTTYG 2 cut(s) 787, 819
Asp700I GAANNNNTTC 2 cut(s) 144, 388
AspS9I GGNCC 1 cut(s) 764
AsuHPI GGTGA 2 cut(s) 49, 334
AvaII GGWCC 1 cut(s) 764
BaeI ACNNNNGTAYC 2 cut(s) 637, 670
BalI TGGCCA 2 cut(s) 261, 368
BbvI GCAGC 2 cut(s) 232, 478
BccI CCATC 3 cut(s) 273, 460, 644
BciT130I CCWGG 1 cut(s) 768
BcoDI GTCTC 1 cut(s) 51
BisI GCNGC 2 cut(s) 246, 492
BlsI GCNGC 2 cut(s) 247, 493
Bme1390I CCNGG 1 cut(s) 768
Bme18I GGWCC 1 cut(s) 764
BmgT120I GGNCC 1 cut(s) 764
BmrFI CCNGG 1 cut(s) 768
BmrI ACTGGG 1 cut(s) 74
BmsI GCATC 2 cut(s) 550, 724
BmuI ACTGGG 1 cut(s) 74
BplI GAGNNNNNCTC 4 cut(s) 217, 249, 219, 251
BsaJI CCNNGG 1 cut(s) 495
Bse1I ACTGG 2 cut(s) 69, 735
BseBI CCWGG 1 cut(s) 768
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 4 cut(s) 165, 471, 517, 739
BseMII CTCAG 1 cut(s) 536
BseNI ACTGG 2 cut(s) 69, 735
BseXI GCAGC 2 cut(s) 232, 478
Bsh1236I CGCG 2 cut(s) 396, 558
BshFI GGCC 3 cut(s) 261, 368, 500
BsmAI GTCTC 1 cut(s) 51
BsnI GGCC 3 cut(s) 261, 368, 500
Bsp143I GATC 4 cut(s) 42, 100, 431, 577
Bsp68I TCGCGA 1 cut(s) 558
BspANI GGCC 3 cut(s) 261, 368, 500
BspCNI CTCAG 1 cut(s) 535
BspFNI CGCG 2 cut(s) 396, 558
BspPI GGATC 2 cut(s) 95, 439
BsrI ACTGG 2 cut(s) 69, 735
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 4 cut(s) 42, 100, 431, 577
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 768
BstC8I GCNNGC 1 cut(s) 823
BstDEI CTNAG 1 cut(s) 522
BstF5I GGATG 4 cut(s) 165, 471, 517, 739
BstFNI CGCG 2 cut(s) 396, 558
BstKTI GATC 4 cut(s) 45, 103, 434, 580
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 4 cut(s) 42, 100, 431, 577
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 1 cut(s) 768
BstNSI RCATGY 4 cut(s) 77, 113, 365, 825
BstSCI CCNGG 1 cut(s) 766
BstUI CGCG 2 cut(s) 396, 558
BstV1I GCAGC 2 cut(s) 232, 478
BsuRI GGCC 3 cut(s) 261, 368, 500
BtgZI GCGATG 1 cut(s) 573
BtsCI GGATG 4 cut(s) 165, 471, 517, 739
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 1 cut(s) 227
BtuMI TCGCGA 1 cut(s) 558
Cac8I GCNNGC 1 cut(s) 823
Cfr13I GGNCC 1 cut(s) 764
CviAII CATG 7 cut(s) 46, 74, 110, 362, 448, 575, 822
DdeI CTNAG 1 cut(s) 522
DpnI GATC 4 cut(s) 44, 102, 433, 579
DpnII GATC 4 cut(s) 42, 100, 431, 577
EaeI YGGCCR 2 cut(s) 259, 366
Eco130I CCWWGG 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 764
EcoRI GAATTC 2 cut(s) 10, 530
EcoRII CCWGG 1 cut(s) 766
EcoT14I CCWWGG 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 7 cut(s) 49, 77, 113, 365, 451, 578, 825
FatI CATG 7 cut(s) 45, 73, 109, 361, 447, 574, 821
FblI GTMKAC 1 cut(s) 805
Fnu4HI GCNGC 2 cut(s) 246, 492
FokI GGATG 4 cut(s) 152, 478, 504, 746
Fsp4HI GCNGC 2 cut(s) 246, 492
GluI GCNGC 2 cut(s) 246, 492
HaeIII GGCC 3 cut(s) 261, 368, 500
Hin1II CATG 7 cut(s) 49, 77, 113, 365, 451, 578, 825
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 1 cut(s) 384
HphI GGTGA 2 cut(s) 49, 334
Hpy166II GTNNAC 4 cut(s) 572, 646, 706, 806
Hpy188I TCNGA 2 cut(s) 150, 610
Hpy188III TCNNGA 5 cut(s) 26, 115, 401, 557, 616
Hpy8I GTNNAC 4 cut(s) 572, 646, 706, 806
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 1 cut(s) 114
HpyCH4IV ACGT 5 cut(s) 96, 193, 552, 711, 802
HpyCH4V TGCA 3 cut(s) 248, 592, 737
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 5 cut(s) 96, 193, 552, 711, 802
Hsp92II CATG 7 cut(s) 49, 77, 113, 365, 451, 578, 825
Kzo9I GATC 4 cut(s) 42, 100, 431, 577
Lsp1109I GCAGC 2 cut(s) 232, 478
LweI GCATC 2 cut(s) 550, 724
MaeII ACGT 5 cut(s) 96, 193, 552, 711, 802
MalI GATC 4 cut(s) 44, 102, 433, 579
MboI GATC 4 cut(s) 42, 100, 431, 577
MboII GAAGA 2 cut(s) 52, 185
MlsI TGGCCA 2 cut(s) 261, 368
MluI ACGCGT 1 cut(s) 394
MluNI TGGCCA 2 cut(s) 261, 368
MmeI TCCRAC 3 cut(s) 361, 433, 724
MnlI CCTC 2 cut(s) 223, 531
Mox20I TGGCCA 2 cut(s) 261, 368
MroXI GAANNNNTTC 2 cut(s) 144, 388
MscI TGGCCA 2 cut(s) 261, 368
MseI TTAA 3 cut(s) 306, 318, 829
Msp20I TGGCCA 2 cut(s) 261, 368
MspA1I CMGCKG 1 cut(s) 491
MspR9I CCNGG 1 cut(s) 768
MvaI CCWGG 1 cut(s) 768
MvnI CGCG 2 cut(s) 396, 558
MwoI GCNNNNNNNGC 1 cut(s) 497
NdeII GATC 4 cut(s) 42, 100, 431, 577
NlaIII CATG 7 cut(s) 49, 77, 113, 365, 451, 578, 825
NruI TCGCGA 1 cut(s) 558
NspI RCATGY 4 cut(s) 77, 113, 365, 825
PaeI GCATGC 1 cut(s) 825
PciI ACATGT 2 cut(s) 73, 361
PcsI WCGNNNNNNNCGW 3 cut(s) 654, 708, 799
PdmI GAANNNNTTC 2 cut(s) 144, 388
PfeI GAWTC 1 cut(s) 384
PfoI TCCNGGA 1 cut(s) 766
PkrI GCNGC 2 cut(s) 247, 493
PscI ACATGT 2 cut(s) 73, 361
PsiI TTATAA 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 766
PspGI CCWGG 1 cut(s) 766
PspPI GGNCC 1 cut(s) 764
PsrI GAACNNNNNNTAC 2 cut(s) 792, 824
PvuII CAGCTG 1 cut(s) 491
RruI TCGCGA 1 cut(s) 558
SaqAI TTAA 3 cut(s) 306, 318, 829
SatI GCNGC 2 cut(s) 246, 492
Sau3AI GATC 4 cut(s) 42, 100, 431, 577
Sau96I GGNCC 1 cut(s) 764
ScrFI CCNGG 1 cut(s) 768
SetI ASST 9 cut(s) 36, 99, 196, 202, 493, 555, 663, 714, 805
SfaNI GCATC 2 cut(s) 550, 724
SinI GGWCC 1 cut(s) 764
SphI GCATGC 1 cut(s) 825
SspI AATATT 2 cut(s) 87, 328
StyD4I CCNGG 1 cut(s) 766
StyI CCWWGG 1 cut(s) 495
TaiI ACGT 5 cut(s) 99, 196, 555, 714, 805
TaqI TCGA 3 cut(s) 14, 657, 679
TfiI GAWTC 1 cut(s) 384
Tru1I TTAA 3 cut(s) 306, 318, 829
Tru9I TTAA 3 cut(s) 306, 318, 829
TscAI CASTG 1 cut(s) 227
TseI GCWGC 2 cut(s) 245, 491
TspDTI ATGAA 3 cut(s) 292, 506, 813
TspGWI ACGGA 2 cut(s) 141, 158
TspRI CASTG 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 764
XapI RAATTY 6 cut(s) 10, 250, 456, 513, 530, 814
XceI RCATGY 4 cut(s) 77, 113, 365, 825
XmiI GTMKAC 1 cut(s) 805
XmnI GAANNNNTTC 2 cut(s) 144, 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.