pycom02g02120

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013733
Physical Location & Seq
Reverse (-)
1264913 .. 1265458
546 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02120.1

Sequence Viewer

Length: 546 bp
ATGGCGGTGGATACTGCCATGACAGCCCACGAAGCCTCCTTTTCGTCCTCCTCCAAGTCCAAACTCTGGAAGTACGACGTATTCTTGAGCTTCAAGGGTGAAGACACCCGTAATAGCTTCACGAGCCACCTCCATGCGGCATTAAAAGCCCGAGGGTACCAGGTCTTTATTGATGAGGACGATCTACAAAGAGGGGAAGAAATAAAAGAGAAACTGTTTCGTGCAATCGAAGAGTCAAGGATCTCTGTCATTATCTTCTCAAAGATGTATGCAGATTCGAGTTGGTGTCTTGACGAGCTGGTGAAGATCATGGAGTGTAGAGACAAACTGGGGCAACATGTTTTACCAATATTCTGTCACGTTGATCCTTCGCATGTCAGGAAGCACGACGGAGATTTAGCCGAAGCATTTCAGAAGCACGAAAAGGACATCCGTGAAGAAAAAGATGACAAGAAACGTGAAGCTAAACAAGAAAGGCTAAAGCTGTGGAGAGAGGCTCTCACAAAAGCTTCAAATTTGTCTGGCCACCATCTTAACAATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

182

Amino Acids

20.96

Weight (kDa)

6.55

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 179 9.4e-49 TIR domain
TIR_2 PF13676 27 - 125 9.5e-20 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 156
AccB1I GGYRCC 1 cut(s) 156
AccB7I CCANNNNNTGG 1 cut(s) 66
AciI CCGC 2 cut(s) 5, 137
AclWI GGATC 2 cut(s) 248, 359
AcoI YGGCCR 1 cut(s) 523
AcsI RAATTY 1 cut(s) 514
AfaI GTAC 2 cut(s) 74, 158
AfiI CCNNNNNNNGG 2 cut(s) 66, 136
AflIII ACRYGT 1 cut(s) 337
AgsI TTSAA 2 cut(s) 94, 513
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 6 cut(s) 90, 117, 298, 464, 484, 509
AluI AGCT 6 cut(s) 90, 117, 298, 464, 484, 509
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 2 cut(s) 248, 359
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 1 cut(s) 523
ApoI RAATTY 1 cut(s) 514
Asp700I GAANNNNTTC 1 cut(s) 408
Asp718I GGTACC 1 cut(s) 156
AsuHPI GGTGA 2 cut(s) 110, 313
AvaI CYCGRG 1 cut(s) 150
BalI TGGCCA 1 cut(s) 525
BanI GGYRCC 1 cut(s) 156
BauI CACGAG 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 108
BccI CCATC 1 cut(s) 537
BciT130I CCWGG 1 cut(s) 161
BciVI GTATCC 1 cut(s) 4
BcoDI GTCTC 1 cut(s) 315
BfuI GTATCC 1 cut(s) 4
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 158
BmrFI CCNGG 1 cut(s) 161
BmrI ACTGGG 1 cut(s) 338
BmuI ACTGGG 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 108
BplI GAGNNNNNCTC 4 cut(s) 481, 513, 483, 515
BpuEI CTTGAG 1 cut(s) 106
BsaJI CCNNGG 1 cut(s) 151
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 136
Bse1I ACTGG 1 cut(s) 333
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 2 cut(s) 66, 136
BseNI ACTGG 1 cut(s) 333
BseRI GAGGAG 1 cut(s) 40
BshFI GGCC 1 cut(s) 525
BshNI GGYRCC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 150
BslI CCNNNNNNNGG 2 cut(s) 66, 136
BsmAI GTCTC 1 cut(s) 315
BsnI GGCC 1 cut(s) 525
BsoBI CYCGRG 1 cut(s) 150
Bsp143I GATC 4 cut(s) 181, 240, 306, 364
BspACI CCGC 2 cut(s) 5, 137
BspANI GGCC 1 cut(s) 525
BspLI GGNNCC 1 cut(s) 158
BspPI GGATC 2 cut(s) 248, 359
BspT107I GGYRCC 1 cut(s) 156
BsrI ACTGG 1 cut(s) 333
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 4 cut(s) 181, 240, 306, 364
BssSI CACGAG 1 cut(s) 121
Bst2BI CACGAG 1 cut(s) 121
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 216
Bst6I CTCTTC 1 cut(s) 225
BstF5I GGATG 1 cut(s) 429
BstKTI GATC 4 cut(s) 184, 243, 309, 367
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 4 cut(s) 181, 240, 306, 364
BstMWI GCNNNNNNNGC 4 cut(s) 23, 32, 123, 146
BstNI CCWGG 1 cut(s) 161
BstNSI RCATGY 2 cut(s) 341, 377
BstSCI CCNGG 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 240
BstYI RGATCY 1 cut(s) 240
BsuI GTATCC 1 cut(s) 4
BsuRI GGCC 1 cut(s) 525
BtsCI GGATG 1 cut(s) 429
CsiI ACCWGGT 1 cut(s) 159
Csp6I GTAC 2 cut(s) 73, 157
CviAII CATG 5 cut(s) 19, 134, 310, 338, 374
CviQI GTAC 2 cut(s) 73, 157
DpnI GATC 4 cut(s) 183, 242, 308, 366
DpnII GATC 4 cut(s) 181, 240, 306, 364
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 159
FaeI CATG 5 cut(s) 22, 137, 313, 341, 377
FaiI YATR 6 cut(s) 20, 135, 270, 311, 339, 375
FatI CATG 5 cut(s) 18, 133, 309, 337, 373
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 138
HaeIII GGCC 1 cut(s) 525
Hin1II CATG 5 cut(s) 22, 137, 313, 341, 377
HindIII AAGCTT 1 cut(s) 507
HinfI GANTC 2 cut(s) 233, 275
HphI GGTGA 2 cut(s) 110, 313
Hpy188I TCNGA 1 cut(s) 414
Hpy188III TCNNGA 5 cut(s) 67, 85, 121, 290, 379
Hpy99I CGWCG 2 cut(s) 80, 392
HpyAV CCTTC 1 cut(s) 378
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4IV ACGT 3 cut(s) 78, 360, 457
HpyCH4V TGCA 2 cut(s) 224, 272
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 32, 123, 146
HpySE526I ACGT 3 cut(s) 78, 360, 457
Hsp92II CATG 5 cut(s) 22, 137, 313, 341, 377
KpnI GGTACC 1 cut(s) 160
Kzo9I GATC 4 cut(s) 181, 240, 306, 364
LpnPI CCDG 7 cut(s) 52, 146, 173, 284, 314, 364, 507
MabI ACCWGGT 1 cut(s) 159
MaeII ACGT 3 cut(s) 78, 360, 457
MaeIII GTNAC 1 cut(s) 356
MalI GATC 4 cut(s) 183, 242, 308, 366
MboI GATC 4 cut(s) 181, 240, 306, 364
MboII GAAGA 6 cut(s) 113, 209, 242, 247, 316, 449
MflI RGATCY 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 525
MluCI AATT 1 cut(s) 514
MluNI TGGCCA 1 cut(s) 525
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 8 cut(s) 46, 58, 61, 140, 146, 169, 185, 487
Mox20I TGGCCA 1 cut(s) 525
MroXI GAANNNNTTC 1 cut(s) 408
MscI TGGCCA 1 cut(s) 525
MseI TTAA 2 cut(s) 143, 534
MslI CAYNNNNRTG 1 cut(s) 132
Msp20I TGGCCA 1 cut(s) 525
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 4 cut(s) 23, 32, 123, 146
NdeII GATC 4 cut(s) 181, 240, 306, 364
NlaIII CATG 5 cut(s) 22, 137, 313, 341, 377
NlaIV GGNNCC 1 cut(s) 158
NmuCI GTSAC 1 cut(s) 356
NspI RCATGY 2 cut(s) 341, 377
PciI ACATGT 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 1 cut(s) 275
PflMI CCANNNNNTGG 1 cut(s) 66
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PscI ACATGT 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 158
PsuI RGATCY 1 cut(s) 240
RsaI GTAC 2 cut(s) 74, 158
RsaNI GTAC 2 cut(s) 73, 157
RseI CAYNNNNRTG 1 cut(s) 132
SaqAI TTAA 2 cut(s) 143, 534
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 4 cut(s) 181, 240, 306, 364
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 161
SexAI ACCWGGT 1 cut(s) 159
SmiMI CAYNNNNRTG 1 cut(s) 132
SmlI CTYRAG 1 cut(s) 85
SmoI CTYRAG 1 cut(s) 85
Sse9I AATT 1 cut(s) 514
SsiI CCGC 2 cut(s) 5, 137
SspI AATATT 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 216
TaiI ACGT 3 cut(s) 81, 363, 460
TaqI TCGA 2 cut(s) 228, 278
TasI AATT 1 cut(s) 514
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 2 cut(s) 143, 534
Tru9I TTAA 2 cut(s) 143, 534
TseFI GTSAC 1 cut(s) 356
Tsp45I GTSAC 1 cut(s) 356
TspGWI ACGGA 2 cut(s) 405, 422
Van91I CCANNNNNTGG 1 cut(s) 66
XapI RAATTY 1 cut(s) 514
XceI RCATGY 2 cut(s) 341, 377
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.