Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004427

Physical Location & Seq

Genomic Coordinates Reverse (-)

22709016 .. 22715100

Gene Structure 6085 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1108200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1431 bp

ATGAGGAATCCGAACCCTCTTTCTCTTCGGTCTCCTCTCTGCTCTGATTTCAGTAGCTGCCGAATCCTGAACGCTTTGAAAACCCAGATTCCATCTCGGTTCTGTTCAGTCATGGGCTCCGATGCTGATATGGAGGATTACGGATTCGAGTACTCGGATGAGGAACCTGAGGAACAGGACGTCGACATTGAGAACCAATATTACAACTCTAAGGGTTTGGTTGAGACAGATCCAGAAGGAGCACTTTCGGGATTCGATGAAGTTGTAAGCATGGAGCCTGAGAAGGCCGAGTGGGGATTTAAAGCCTTGAAGCAAACCGTGAAGCTCTATTATCGTCTGGGGAGGTATAAAGAAATGATGGATGCATACAGGGTGATGTTGACATACATCAAATCAGCAGTAACAAGGAATTACAGTGAAAAATGTATAAACAACATTATGGATTTTGTTTCTGGTTCAGCTAGTCAGAACTTTGGTCTCCTGCAAGAGTTCTATCAGACCACTCTAAAGGCCCTTGAAGAGGCAAAGAATGAGAGACTTTGGTTTAAGACAAATCTTAAGCTTTGCAAAATTTGGTTTGATATGGGTGAATATGGGCGAATGAGTAAGATTTTGAAGGAACTCCATAAATCCTGTCAAAAGGACGATGGTACTGATGACCAAAAGAAAGGAAGTCAACTCCTAGAGGTTTACGCTATTGAGATTCAAATGTACACTGAGACTAAAAATAACAAAAAGCTTAAGCAATTATACCAAAAAGCTCTTGCAATCAAGTCAGCAATACCTCATCCTAGGATCATGGGAATAATTCGTGAGTGTGGGGGTAAGATGCATATGGCTGAGCGTCAGTGGGCAGATGCAGCCACAGATTTTTTTGAAGCCTTTAAGAACTATGATGAAGCTGGGAACCAGAGGCGTATACAATGCTTGAAGTATTTAGTTCTGGCTAATATGCTGATGGAGTCAGAAGTTAATCCATTTGATGGTCAAGAAGCAAAGCCGTATAAAAACGATCCTGAGATCTTGGCAATGACAAATCTGATTGCAGCATATCAACGAAATGAGATACTGGAGTTTGAGAAAATTCTTAAGAGTAACAGGAGGACAATTATGGATGATCCATTCATCCGAAACTATATTGAAGATCTGTTGAAGAATGTTAGGACACAAGTGCTGCTCAAACTTATCAAGCCATACACGAGAATTCGGATTCCCTTCATATCAAAGGAACTTAATGTTCCGGAGAAAGATGTGGAGCAACTTTTGGTTTCACTTATATTGGATAACCGAATTGATGGACATATTGATCAGGTGAACCGGCTCTTAGAGCGTGGTGACAGGTCAAAGGGAATGAAGAAGTATGCTGCCGTAGATAAATGGAACACTCAGCTAAGGTCTCTTTACCAAACTATCAGTAACCGAGTATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000338 GO:0003674 GO:0003712 GO:0003714 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0019538 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0080090 GO:0140110 GO:1901564 GO:1901565 GO:1901575 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PCI

Protein Analysis

477

Amino Acids

55.49

Weight (kDa)

6.93

Isoelectric Point (pI)

37.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCI	PF01399	363 - 443	1.8e-23	PCI domain
COPS2_C	PF25983	451 - 475	1.8e-13	COP9 signalosome complex subunit 2, C-terminal helix

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26990
fragaria_vesca	FvH4_4g27570
malus_domestica	MD00G1108200.v1.1 MD03G1163800.v1.1 MD11G1176300.v1.1
prunus_persica	Prupe.6G156800_v2.0.a1 Prupe.6G156800_v2.0.a1
pyrus_communis	pycom11g15960 pycom11g16000
rosa_chinensis	RchiOBHm_Chr4g0435421 RchiOBHm_Chr5g0050051
rosa_laevigata	RLG00000006594 RLG00000034682
rosa_multiflora	Rmu_sc0000333.1_g000008 Rmu_sc0001940.1_g000023 Rmu_sc0009623.1_g000002 Rmu_sc0016267.1_g000001 Rmu_sc0036582.1_g000002
rosa_roxburghii	Rroxscaffold_1G00031080 Rroxscaffold_5G00376660
rosa_rugosa	Rorug04G0284900 Rorug05G0256900
rosa_samantha	Rh4AG340600 Rh4BG348900 Rh4CG363400 Rh4DG343100 Rh5AG330100 Rh5BG340800 Rh5CG367400 Rh5DG353500
rosa_wichuraiana	Rw4G029700 Rw5G031150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	183
AccB7I	CCANNNNNTGG	1 cut(s)	983
AccI	GTMKAC	2 cut(s)	183, 919
AccIII	TCCGGA	1 cut(s)	1240
AclWI	GGATC	4 cut(s)	224, 803, 1007, 1112
AcsI	RAATTY	3 cut(s)	570, 1083, 1203
AcyI	GRCGYC	1 cut(s)	180
AfaI	GTAC	3 cut(s)	152, 652, 713
AfiI	CCNNNNNNNGG	2 cut(s)	520, 983
AflII	CTTAAG	3 cut(s)	557, 740, 1088
AgsI	TTSAA	9 cut(s)	79, 310, 518, 616, 707, 878, 931, 1142, 1153
AluBI	AGCT	8 cut(s)	57, 325, 461, 562, 739, 761, 902, 1390
AluI	AGCT	8 cut(s)	57, 325, 461, 562, 739, 761, 902, 1390
Alw21I	GWGCWC	1 cut(s)	244
Alw26I	GTCTC	6 cut(s)	36, 218, 482, 529, 713, 1401
AlwI	GGATC	4 cut(s)	224, 803, 1007, 1112
AlwNI	CAGNNNCTG	1 cut(s)	57
Aor13HI	TCCGGA	1 cut(s)	1240
AoxI	GGCC	2 cut(s)	285, 510
ApeKI	GCWGC	5 cut(s)	57, 860, 1046, 1174, 1364
ApoI	RAATTY	3 cut(s)	570, 1083, 1203
AspA2I	CCTAGG	1 cut(s)	791
AspS9I	GGNCC	1 cut(s)	511
AsuHPI	GGTGA	4 cut(s)	385, 599, 1324, 1346
AvrII	CCTAGG	1 cut(s)	791
AxyI	CCTNAGG	1 cut(s)	168
BaeI	ACNNNNGTAYC	2 cut(s)	1058, 1091
BanII	GRGCYC	1 cut(s)	119
BauI	CACGAG	1 cut(s)	1198
Bbv12I	GWGCWC	1 cut(s)	244
BbvI	GCAGC	5 cut(s)	44, 872, 1058, 1161, 1351
BccI	CCATC	6 cut(s)	100, 352, 641, 952, 977, 1289
BceAI	ACGGC	2 cut(s)	985, 1352
BclI	TGATCA	1 cut(s)	1306
BcoDI	GTCTC	6 cut(s)	36, 218, 482, 529, 713, 1401
BfaI	CTAG	3 cut(s)	462, 683, 792
BfrI	CTTAAG	3 cut(s)	557, 740, 1088
BglII	AGATCT	2 cut(s)	1020, 1144
BisI	GCNGC	5 cut(s)	58, 861, 1047, 1175, 1365
BlnI	CCTAGG	1 cut(s)	791
BlpI	GCTNAGC	1 cut(s)	840
BlsI	GCNGC	5 cut(s)	59, 862, 1048, 1176, 1366
BmcAI	AGTACT	1 cut(s)	152
BmgT120I	GGNCC	1 cut(s)	511
BmiI	GGNNCC	4 cut(s)	118, 165, 276, 908
BmsI	GCATC	4 cut(s)	112, 352, 819, 847
BpmI	CTGGAG	1 cut(s)	1091
Bpu10I	CCTNAGC	1 cut(s)	1391
Bpu1102I	GCTNAGC	1 cut(s)	840
BsaHI	GRCGYC	1 cut(s)	180
BsaI	GGTCTC	3 cut(s)	36, 482, 1401
BsaJI	CCNNGG	1 cut(s)	791
BsaWI	WCCGGW	1 cut(s)	1240
Bsc4I	CCNNNNNNNGG	2 cut(s)	520, 983
Bse118I	RCCGGY	1 cut(s)	1317
Bse1I	ACTGG	1 cut(s)	1074
Bse21I	CCTNAGG	1 cut(s)	168
Bse3DI	GCAATG	1 cut(s)	1035
BseAI	TCCGGA	1 cut(s)	1240
BseDI	CCNNGG	1 cut(s)	791
BseGI	GGATG	5 cut(s)	163, 367, 787, 1120, 1125
BseLI	CCNNNNNNNGG	2 cut(s)	520, 983
BseMI	GCAATG	1 cut(s)	1035
BseMII	CTCAG	6 cut(s)	159, 270, 708, 831, 1008, 1400
BseNI	ACTGG	1 cut(s)	1074
BseRI	GAGGAG	1 cut(s)	24
BseXI	GCAGC	5 cut(s)	44, 872, 1058, 1161, 1351
BseYI	CCCAGC	1 cut(s)	902
BshFI	GGCC	2 cut(s)	287, 512
BsiHKAI	GWGCWC	1 cut(s)	244
BsiSI	CCGG	2 cut(s)	1241, 1318
BslI	CCNNNNNNNGG	2 cut(s)	520, 983
BsmAI	GTCTC	6 cut(s)	36, 218, 482, 529, 713, 1401
BsnI	GGCC	2 cut(s)	287, 512
Bso31I	GGTCTC	3 cut(s)	36, 482, 1401
Bsp1286I	GDGCHC	2 cut(s)	119, 244
Bsp13I	TCCGGA	1 cut(s)	1240
Bsp1407I	TGTACA	1 cut(s)	711
Bsp143I	GATC	7 cut(s)	229, 795, 1012, 1020, 1117, 1144, 1306
Bsp1720I	GCTNAGC	1 cut(s)	840
BspANI	GGCC	2 cut(s)	287, 512
BspCNI	CTCAG	6 cut(s)	160, 271, 709, 832, 1009, 1399
BspEI	TCCGGA	1 cut(s)	1240
BspLI	GGNNCC	4 cut(s)	118, 165, 276, 908
BspPI	GGATC	4 cut(s)	224, 803, 1007, 1112
BspTI	CTTAAG	3 cut(s)	557, 740, 1088
BspTNI	GGTCTC	3 cut(s)	36, 482, 1401
BsrDI	GCAATG	1 cut(s)	1035
BsrFI	RCCGGY	1 cut(s)	1317
BsrGI	TGTACA	1 cut(s)	711
BsrI	ACTGG	1 cut(s)	1074
BssAI	RCCGGY	1 cut(s)	1317
BssECI	CCNNGG	1 cut(s)	791
BssMI	GATC	7 cut(s)	229, 795, 1012, 1020, 1117, 1144, 1306
BssNAI	GTATAC	1 cut(s)	920
BssNI	GRCGYC	1 cut(s)	180
BssSI	CACGAG	1 cut(s)	1198
BssT1I	CCWWGG	1 cut(s)	791
Bst1107I	GTATAC	1 cut(s)	920
Bst2BI	CACGAG	1 cut(s)	1198
Bst4CI	ACNGT	2 cut(s)	319, 416
Bst6I	CTCTTC	2 cut(s)	30, 513
BstACI	GRCGYC	1 cut(s)	180
BstAFI	CTTAAG	3 cut(s)	557, 740, 1088
BstAUI	TGTACA	1 cut(s)	711
BstDEI	CTNAG	9 cut(s)	168, 210, 279, 717, 840, 1017, 1324, 1386, 1391
BstENI	CCTNNNNNAGG	1 cut(s)	518
BstF5I	GGATG	5 cut(s)	163, 367, 787, 1120, 1125
BstKTI	GATC	7 cut(s)	232, 798, 1015, 1023, 1120, 1147, 1309
BstMAI	GTCTC	6 cut(s)	36, 218, 482, 529, 713, 1401
BstMBI	GATC	7 cut(s)	229, 795, 1012, 1020, 1117, 1144, 1306
BstMWI	GCNNNNNNNGC	2 cut(s)	860, 1327
BstV1I	GCAGC	5 cut(s)	44, 872, 1058, 1161, 1351
BstX2I	RGATCY	3 cut(s)	229, 1020, 1144
BstYI	RGATCY	3 cut(s)	229, 1020, 1144
BstZ17I	GTATAC	1 cut(s)	920
Bsu36I	CCTNAGG	1 cut(s)	168
BsuRI	GGCC	2 cut(s)	287, 512
BtsCI	GGATG	5 cut(s)	163, 367, 787, 1120, 1125
BtsIMutI	CAGTG	3 cut(s)	421, 714, 854
CaiI	CAGNNNCTG	1 cut(s)	57
Cfr10I	RCCGGY	1 cut(s)	1317
Cfr13I	GGNCC	1 cut(s)	511
CseI	GACGC	1 cut(s)	833
Csp6I	GTAC	3 cut(s)	151, 651, 712
CviAII	CATG	3 cut(s)	112, 271, 799
CviQI	GTAC	3 cut(s)	151, 651, 712
DdeI	CTNAG	9 cut(s)	168, 210, 279, 717, 840, 1017, 1324, 1386, 1391
DpnI	GATC	7 cut(s)	231, 797, 1014, 1022, 1119, 1146, 1308
DpnII	GATC	7 cut(s)	229, 795, 1012, 1020, 1117, 1144, 1306
DraI	TTTAAA	1 cut(s)	301
Eam1104I	CTCTTC	2 cut(s)	30, 513
EarI	CTCTTC	2 cut(s)	30, 513
Eco130I	CCWWGG	1 cut(s)	791
Eco24I	GRGCYC	1 cut(s)	119
Eco31I	GGTCTC	3 cut(s)	36, 482, 1401
Eco81I	CCTNAGG	1 cut(s)	168
EcoNI	CCTNNNNNAGG	1 cut(s)	518
EcoO109I	RGGNCCY	1 cut(s)	511
EcoRI	GAATTC	1 cut(s)	1203
EcoT14I	CCWWGG	1 cut(s)	791
EcoT22I	ATGCAT	2 cut(s)	367, 834
EcoT38I	GRGCYC	1 cut(s)	119
ErhI	CCWWGG	1 cut(s)	791
FaeI	CATG	3 cut(s)	115, 274, 802
FalI	AAGNNNNNCTT	2 cut(s)	228, 260
FatI	CATG	3 cut(s)	111, 270, 798
FauNDI	CATATG	1 cut(s)	834
FbaI	TGATCA	1 cut(s)	1306
FblI	GTMKAC	2 cut(s)	183, 919
Fnu4HI	GCNGC	5 cut(s)	58, 861, 1047, 1175, 1365
FokI	GGATG	5 cut(s)	170, 374, 774, 1112, 1127
FriOI	GRGCYC	1 cut(s)	119
Fsp4HI	GCNGC	5 cut(s)	58, 861, 1047, 1175, 1365
FspBI	CTAG	3 cut(s)	462, 683, 792
GluI	GCNGC	5 cut(s)	58, 861, 1047, 1175, 1365
GsaI	CCCAGC	1 cut(s)	906
GsuI	CTGGAG	1 cut(s)	1091
HaeIII	GGCC	2 cut(s)	287, 512
HapII	CCGG	2 cut(s)	1241, 1318
HgaI	GACGC	1 cut(s)	833
Hin1I	GRCGYC	1 cut(s)	180
Hin1II	CATG	3 cut(s)	115, 274, 802
HincII	GTYRAC	3 cut(s)	184, 381, 677
HindII	GTYRAC	3 cut(s)	184, 381, 677
HindIII	AAGCTT	2 cut(s)	560, 737
HinfI	GANTC	8 cut(s)	7, 63, 88, 144, 252, 703, 962, 1210
HpaII	CCGG	2 cut(s)	1241, 1318
HphI	GGTGA	4 cut(s)	385, 599, 1324, 1346
Hpy166II	GTNNAC	7 cut(s)	184, 381, 677, 691, 714, 920, 1315
Hpy188III	TCNNGA	7 cut(s)	67, 233, 249, 812, 989, 1016, 1241
Hpy8I	GTNNAC	7 cut(s)	184, 381, 677, 691, 714, 920, 1315
Hpy99I	CGWCG	1 cut(s)	185
HpyAV	CCTTC	4 cut(s)	230, 277, 610, 1225
HpyCH4III	ACNGT	2 cut(s)	319, 416
HpyCH4IV	ACGT	1 cut(s)	180
HpyCH4V	TGCA	7 cut(s)	365, 484, 567, 767, 832, 860, 1046
HpyF10VI	GCNNNNNNNGC	2 cut(s)	860, 1327
HpyF3I	CTNAG	9 cut(s)	168, 210, 279, 717, 840, 1017, 1324, 1386, 1391
HpySE526I	ACGT	1 cut(s)	180
Hsp92I	GRCGYC	1 cut(s)	180
Hsp92II	CATG	3 cut(s)	115, 274, 802
Kpn2I	TCCGGA	1 cut(s)	1240
Ksp22I	TGATCA	1 cut(s)	1306
Kzo9I	GATC	7 cut(s)	229, 795, 1012, 1020, 1117, 1144, 1306
LmnI	GCTCC	4 cut(s)	122, 239, 274, 1255
Lsp1109I	GCAGC	5 cut(s)	44, 872, 1058, 1161, 1351
LweI	GCATC	4 cut(s)	112, 352, 819, 847
MaeI	CTAG	3 cut(s)	462, 683, 792
MaeII	ACGT	1 cut(s)	180
MaeIII	GTNAC	4 cut(s)	400, 1094, 1334, 1415
MalI	GATC	7 cut(s)	231, 797, 1014, 1022, 1119, 1146, 1308
MboI	GATC	7 cut(s)	229, 795, 1012, 1020, 1117, 1144, 1306
MboII	GAAGA	5 cut(s)	17, 530, 1154, 1165, 1366
MflI	RGATCY	3 cut(s)	229, 1020, 1144
MhlI	GDGCHC	2 cut(s)	119, 244
MluCI	AATT	8 cut(s)	409, 570, 746, 807, 1083, 1107, 1203, 1290
MlyI	GAGTC	1 cut(s)	971
Mph1103I	ATGCAT	2 cut(s)	367, 834
MroI	TCCGGA	1 cut(s)	1240
MseI	TTAA	8 cut(s)	300, 546, 558, 741, 885, 972, 1089, 1233
MspCI	CTTAAG	3 cut(s)	557, 740, 1088
MspI	CCGG	2 cut(s)	1241, 1318
MwoI	GCNNNNNNNGC	2 cut(s)	860, 1327
NdeI	CATATG	1 cut(s)	834
NdeII	GATC	7 cut(s)	229, 795, 1012, 1020, 1117, 1144, 1306
NlaIII	CATG	3 cut(s)	115, 274, 802
NlaIV	GGNNCC	4 cut(s)	118, 165, 276, 908
NmeAIII	GCCGAG	1 cut(s)	313
NmuCI	GTSAC	1 cut(s)	1334
NsiI	ATGCAT	2 cut(s)	367, 834
PfeI	GAWTC	7 cut(s)	7, 63, 88, 144, 252, 703, 1210
PflMI	CCANNNNNTGG	1 cut(s)	983
PkrI	GCNGC	5 cut(s)	59, 862, 1048, 1176, 1366
PleI	GAGTC	1 cut(s)	970
PpsI	GAGTC	1 cut(s)	970
PspFI	CCCAGC	1 cut(s)	902
PspN4I	GGNNCC	4 cut(s)	118, 165, 276, 908
PspPI	GGNCC	1 cut(s)	511
PsrI	GAACNNNNNNTAC	2 cut(s)	185, 217
PstNI	CAGNNNCTG	1 cut(s)	57
PsuI	RGATCY	3 cut(s)	229, 1020, 1144
RsaI	GTAC	3 cut(s)	152, 652, 713
RsaNI	GTAC	3 cut(s)	151, 651, 712
SalI	GTCGAC	1 cut(s)	182
SaqAI	TTAA	8 cut(s)	300, 546, 558, 741, 885, 972, 1089, 1233
SatI	GCNGC	5 cut(s)	58, 861, 1047, 1175, 1365
Sau3AI	GATC	7 cut(s)	229, 795, 1012, 1020, 1117, 1144, 1306
Sau96I	GGNCC	1 cut(s)	511
ScaI	AGTACT	1 cut(s)	152
SchI	GAGTC	1 cut(s)	971
SduI	GDGCHC	2 cut(s)	119, 244
SfaNI	GCATC	4 cut(s)	112, 352, 819, 847
SmlI	CTYRAG	3 cut(s)	557, 740, 1088
SmoI	CTYRAG	3 cut(s)	557, 740, 1088
Sse9I	AATT	8 cut(s)	409, 570, 746, 807, 1083, 1107, 1203, 1290
SspI	AATATT	1 cut(s)	200
SspMI	CTAG	3 cut(s)	462, 683, 792
StyI	CCWWGG	1 cut(s)	791
TaaI	ACNGT	2 cut(s)	319, 416
TaiI	ACGT	1 cut(s)	183
TaqI	TCGA	3 cut(s)	147, 183, 255
TaqII	GACCGA	1 cut(s)	18
TasI	AATT	8 cut(s)	409, 570, 746, 807, 1083, 1107, 1203, 1290
TatI	WGTACW	2 cut(s)	150, 711
TfiI	GAWTC	7 cut(s)	7, 63, 88, 144, 252, 703, 1210
Tru1I	TTAA	8 cut(s)	300, 546, 558, 741, 885, 972, 1089, 1233
Tru9I	TTAA	8 cut(s)	300, 546, 558, 741, 885, 972, 1089, 1233
TscAI	CASTG	3 cut(s)	421, 721, 854
TseFI	GTSAC	1 cut(s)	1334
TseI	GCWGC	5 cut(s)	57, 860, 1046, 1174, 1364
Tsp45I	GTSAC	1 cut(s)	1334
TspDTI	ATGAA	5 cut(s)	273, 912, 1114, 1207, 1367
TspGWI	ACGGA	1 cut(s)	156
TspRI	CASTG	3 cut(s)	421, 721, 854
Van91I	CCANNNNNTGG	1 cut(s)	983
Vha464I	CTTAAG	3 cut(s)	557, 740, 1088
XagI	CCTNNNNNAGG	1 cut(s)	518
XapI	RAATTY	3 cut(s)	570, 1083, 1203
XmaJI	CCTAGG	1 cut(s)	791
XmiI	GTMKAC	2 cut(s)	183, 919
XspI	CTAG	3 cut(s)	462, 683, 792
ZraI	GACGTC	1 cut(s)	181
ZrmI	AGTACT	1 cut(s)	152
Zsp2I	ATGCAT	2 cut(s)	367, 834

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.