Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0004427

Physical Location & Seq

Genomic Coordinates Reverse (-)

56428255 .. 56432939

Gene Structure 4685 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G029700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGCAGCGAACATGCAAACTGAATATGCTGATATGGAGGATTACGGATTCGAGTACTCGGACGAAGAGCCTGAGGAGCAGGACGTTGATATTGAGAACCAGTATTACAACTCCAAAGGTTTGGTTGAGACTGATCCAGAAGGAGCGCTTTCAGGATTTGAAGAAGTAATTCGCATGGAGCCTGACAAGGCTGAGTGGGGATTTAAATCTTTGAAGCAAACTGTGAAGATCTATTACCGTCTCAGGAGGTATAAAGAGATGATGGATTCATACAGGGAGATGTTGACATATATCAAATCAGCCGTAACACGGAATTACAGTGAAAAATGTATAAATAACATTATGGATTTTGTGTCCGGGTCAGCTAGTCAGAACTCTGGTCTCCTGCAAGATTTTTATCAGACCACTCTAAAGGCCCTTGAAGAGGCAAAGAATGAGAGGCTTTGGTTCAAGACAAATCTTAAGCTTTGCAAAATTTGGTTTGATATGAGTGAATATGGGCGAATGAGTAAGATTTTGAAGGAACTCCACAAATCTTGTCAAAAGGAAGATGGTACTGATGATCAAAAGAAAGGAAGTCAACTTTTGGAGGTTTATGCAATTGAGATTCAAATGTACACTGAGACTAAAAATAACAAAAAGCTAAAGCAATTATACCAAAAAGCTCTTGCAATCAAGTCAGCAATACCTCATCCTAGAATCATGGGAATAATTCGTGAGTGTGGGGGTAAGATGCATATGACAGAGCGTCAGTGGGCAGAGGCCGCCACAGATTTTTTTGAAGCTTTCAAGAACTATGATGAAGCGGGGAACCAGAGGCGTATACAATGCTTGAAATATTTAGTTCTGGCTAATATGCTGATGCAGTCCGAGGTTAATCCATTTGATGGTCAAGAAGCAAAGCCGTATAAAAATGATCCTGAGATCTTGGCAATGACAAATCTGGTAGCAGCATATCAACGAAATGAGATATTGGAGTTCGAAAAAATTCTGAAGAGTAATAGGAAGACAATTATGGATGATCCATTCATTCGAAACTATATTGAAGATCTGTTGAAGAATATTAGAACACAAGTGCTGCTTAAACTAATCAAGCCGTACACACGAATCCGAATTCCCTTCATATCAAAGGAACTTAATGTCCCTGAGAAAGATGTTGAGCAGCTCTTGGTTTCACTAATTTTAGATAACAAAATCGATGGACATATTGATCAGGTGAACCAGCTCATGGAGCGCGGTGACAGGTCGAAGGGAAGGAAGAAGTATGCTGCCGTAGATAAATGGAACACACAGCTGAAGTCTCTTTCTCAAACTATTGGTAACCGAGTATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000338 GO:0003674 GO:0003712 GO:0003714 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0019538 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0080090 GO:0140110 GO:1901564 GO:1901565 GO:1901575 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PCI

Protein Analysis

444

Amino Acids

52.08

Weight (kDa)

6.26

Isoelectric Point (pI)

39.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCI	PF01399	331 - 411	8.7e-24	PCI domain
COPS2_C	PF25983	420 - 443	3.7e-12	COP9 signalosome complex subunit 2, C-terminal helix

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26990
fragaria_vesca	FvH4_4g27570
malus_domestica	MD00G1108200.v1.1 MD03G1163800.v1.1 MD11G1176300.v1.1
prunus_persica	Prupe.6G156800_v2.0.a1 Prupe.6G156800_v2.0.a1
pyrus_communis	pycom11g15960 pycom11g16000
rosa_chinensis	RchiOBHm_Chr4g0435421 RchiOBHm_Chr5g0050051
rosa_laevigata	RLG00000006594 RLG00000034682
rosa_multiflora	Rmu_sc0000333.1_g000008 Rmu_sc0001940.1_g000023 Rmu_sc0009623.1_g000002 Rmu_sc0016267.1_g000001 Rmu_sc0036582.1_g000002
rosa_roxburghii	Rroxscaffold_1G00031080 Rroxscaffold_5G00376660
rosa_rugosa	Rorug04G0284900 Rorug05G0256900
rosa_samantha	Rh4AG340600 Rh4BG348900 Rh4CG363400 Rh4DG343100 Rh5AG330100 Rh5BG340800 Rh5CG367400 Rh5DG353500
rosa_wichuraiana	Rw4G029700 Rw5G031150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	887, 1228
AccI	GTMKAC	1 cut(s)	823
AccII	CGCG	1 cut(s)	1236
AciI	CCGC	3 cut(s)	765, 806, 1236
AclWI	GGATC	3 cut(s)	128, 911, 1016
AcsI	RAATTY	3 cut(s)	474, 987, 1113
AcuI	CTGAAG	2 cut(s)	1013, 1316
AfaI	GTAC	4 cut(s)	56, 556, 617, 1100
AfeI	AGCGCT	1 cut(s)	147
AfiI	CCNNNNNNNGG	4 cut(s)	309, 424, 887, 1228
AflII	CTTAAG	1 cut(s)	461
AhdI	GACNNNNNGTC	1 cut(s)	747
AloI	GAACNNNNNNTCC	2 cut(s)	1125, 1157
AluBI	AGCT	8 cut(s)	365, 466, 643, 665, 785, 1165, 1225, 1294
AluI	AGCT	8 cut(s)	365, 466, 643, 665, 785, 1165, 1225, 1294
Alw26I	GTCTC	5 cut(s)	122, 245, 386, 617, 1305
AlwI	GGATC	3 cut(s)	128, 911, 1016
Aor51HI	AGCGCT	1 cut(s)	147
AoxI	GGCC	2 cut(s)	414, 762
ApeKI	GCWGC	5 cut(s)	5, 950, 1078, 1162, 1268
ApoI	RAATTY	3 cut(s)	474, 987, 1113
Asp700I	GAANNNNTTC	2 cut(s)	168, 987
AspLEI	GCGC	2 cut(s)	148, 1236
AspS9I	GGNCC	1 cut(s)	415
AsuC2I	CCSGG	1 cut(s)	358
AsuHPI	GGTGA	2 cut(s)	1228, 1250
AsuII	TTCGAA	2 cut(s)	981, 1033
AxyI	CCTNAGG	1 cut(s)	72
BarI	GAAGNNNNNNTAC	2 cut(s)	218, 250
BbsI	GAAGAC	1 cut(s)	1013
BbvI	GCAGC	5 cut(s)	17, 962, 1065, 1174, 1255
BccI	CCATC	4 cut(s)	256, 545, 881, 1193
BceAI	ACGGC	4 cut(s)	287, 889, 1081, 1256
BclI	TGATCA	2 cut(s)	562, 1210
BcnI	CCSGG	1 cut(s)	358
BcoDI	GTCTC	5 cut(s)	122, 245, 386, 617, 1305
BfaI	CTAG	2 cut(s)	366, 696
BfoI	RGCGCY	1 cut(s)	149
BfrI	CTTAAG	1 cut(s)	461
BglII	AGATCT	3 cut(s)	228, 924, 1048
BisI	GCNGC	6 cut(s)	6, 765, 951, 1079, 1163, 1269
BlsI	GCNGC	6 cut(s)	7, 766, 952, 1080, 1164, 1270
BmcAI	AGTACT	1 cut(s)	56
Bme1390I	CCNGG	1 cut(s)	358
BmeRI	GACNNNNNGTC	1 cut(s)	747
BmgT120I	GGNCC	1 cut(s)	415
BmiI	GGNNCC	2 cut(s)	180, 812
BmrFI	CCNGG	1 cut(s)	358
BmsI	GCATC	2 cut(s)	723, 852
BpiI	GAAGAC	1 cut(s)	1013
Bpu14I	TTCGAA	2 cut(s)	981, 1033
BpuMI	CCSGG	1 cut(s)	358
Bsa29I	ATCGAT	1 cut(s)	1197
BsaBI	GATNNNNATC	2 cut(s)	205, 396
BsaI	GGTCTC	1 cut(s)	386
BsaJI	CCNNGG	1 cut(s)	870
Bsc4I	CCNNNNNNNGG	4 cut(s)	309, 424, 887, 1228
Bse1I	ACTGG	1 cut(s)	100
Bse21I	CCTNAGG	1 cut(s)	72
Bse3DI	GCAATG	1 cut(s)	939
Bse8I	GATNNNNATC	2 cut(s)	205, 396
BseCI	ATCGAT	1 cut(s)	1197
BseDI	CCNNGG	1 cut(s)	870
BseGI	GGATG	2 cut(s)	691, 1024
BseJI	GATNNNNATC	2 cut(s)	205, 396
BseLI	CCNNNNNNNGG	4 cut(s)	309, 424, 887, 1228
BseMI	GCAATG	1 cut(s)	939
BseMII	CTCAG	6 cut(s)	63, 183, 256, 612, 912, 1137
BseNI	ACTGG	1 cut(s)	100
BseRI	GAGGAG	1 cut(s)	89
BseXI	GCAGC	5 cut(s)	17, 962, 1065, 1174, 1255
Bsh1236I	CGCG	1 cut(s)	1236
BshFI	GGCC	2 cut(s)	416, 764
BshVI	ATCGAT	1 cut(s)	1197
BsiSI	CCGG	1 cut(s)	357
BslFI	GGGAC	1 cut(s)	1127
BslI	CCNNNNNNNGG	4 cut(s)	309, 424, 887, 1228
BsmAI	GTCTC	5 cut(s)	122, 245, 386, 617, 1305
BsmBI	CGTCTC	1 cut(s)	245
BsmFI	GGGAC	1 cut(s)	1127
BsnI	GGCC	2 cut(s)	416, 764
Bso31I	GGTCTC	1 cut(s)	386
Bsp119I	TTCGAA	2 cut(s)	981, 1033
Bsp1407I	TGTACA	1 cut(s)	615
Bsp143I	GATC	8 cut(s)	133, 228, 562, 916, 924, 1021, 1048, 1210
BspACI	CCGC	3 cut(s)	765, 806, 1236
BspANI	GGCC	2 cut(s)	416, 764
BspCNI	CTCAG	6 cut(s)	64, 184, 255, 613, 913, 1138
BspDI	ATCGAT	1 cut(s)	1197
BspFNI	CGCG	1 cut(s)	1236
BspLI	GGNNCC	2 cut(s)	180, 812
BspPI	GGATC	3 cut(s)	128, 911, 1016
BspQI	GCTCTTC	1 cut(s)	60
BspT104I	TTCGAA	2 cut(s)	981, 1033
BspTI	CTTAAG	1 cut(s)	461
BspTNI	GGTCTC	1 cut(s)	386
BsrDI	GCAATG	1 cut(s)	939
BsrGI	TGTACA	1 cut(s)	615
BsrI	ACTGG	1 cut(s)	100
BssECI	CCNNGG	1 cut(s)	870
BssMI	GATC	8 cut(s)	133, 228, 562, 916, 924, 1021, 1048, 1210
BssNAI	GTATAC	1 cut(s)	824
Bst1107I	GTATAC	1 cut(s)	824
Bst4CI	ACNGT	3 cut(s)	223, 239, 320
Bst6I	CTCTTC	3 cut(s)	60, 417, 989
BstAFI	CTTAAG	1 cut(s)	461
BstAUI	TGTACA	1 cut(s)	615
BstBI	TTCGAA	2 cut(s)	981, 1033
BstDEI	CTNAG	6 cut(s)	72, 192, 242, 621, 921, 1146
BstEII	GGTNACC	1 cut(s)	1319
BstENI	CCTNNNNNAGG	1 cut(s)	422
BstF5I	GGATG	2 cut(s)	691, 1024
BstFNI	CGCG	1 cut(s)	1236
BstH2I	RGCGCY	1 cut(s)	149
BstHHI	GCGC	2 cut(s)	148, 1236
BstKTI	GATC	8 cut(s)	136, 231, 565, 919, 927, 1024, 1051, 1213
BstMAI	GTCTC	5 cut(s)	122, 245, 386, 617, 1305
BstMBI	GATC	8 cut(s)	133, 228, 562, 916, 924, 1021, 1048, 1210
BstMWI	GCNNNNNNNGC	3 cut(s)	76, 764, 1231
BstNSI	RCATGY	1 cut(s)	16
BstPI	GGTNACC	1 cut(s)	1319
BstSCI	CCNGG	1 cut(s)	356
BstUI	CGCG	1 cut(s)	1236
BstV1I	GCAGC	5 cut(s)	17, 962, 1065, 1174, 1255
BstV2I	GAAGAC	1 cut(s)	1013
BstX2I	RGATCY	3 cut(s)	228, 924, 1048
BstXI	CCANNNNNNTGG	1 cut(s)	121
BstYI	RGATCY	3 cut(s)	228, 924, 1048
BstZ17I	GTATAC	1 cut(s)	824
Bsu15I	ATCGAT	1 cut(s)	1197
Bsu36I	CCTNAGG	1 cut(s)	72
BsuRI	GGCC	2 cut(s)	416, 764
BsuTUI	ATCGAT	1 cut(s)	1197
BtsCI	GGATG	2 cut(s)	691, 1024
BtsIMutI	CAGTG	3 cut(s)	325, 618, 758
CfoI	GCGC	2 cut(s)	148, 1236
Cfr13I	GGNCC	1 cut(s)	415
ClaI	ATCGAT	1 cut(s)	1197
CseI	GACGC	1 cut(s)	737
Csp6I	GTAC	4 cut(s)	55, 555, 616, 1099
CspCI	CAANNNNNGTGG	2 cut(s)	518, 553
CviAII	CATG	4 cut(s)	13, 175, 703, 1228
CviQI	GTAC	4 cut(s)	55, 555, 616, 1099
DdeI	CTNAG	6 cut(s)	72, 192, 242, 621, 921, 1146
DpnI	GATC	8 cut(s)	135, 230, 564, 918, 926, 1023, 1050, 1212
DpnII	GATC	8 cut(s)	133, 228, 562, 916, 924, 1021, 1048, 1210
DraI	TTTAAA	1 cut(s)	205
DriI	GACNNNNNGTC	1 cut(s)	747
Eam1104I	CTCTTC	3 cut(s)	60, 417, 989
Eam1105I	GACNNNNNGTC	1 cut(s)	747
EarI	CTCTTC	3 cut(s)	60, 417, 989
Eco31I	GGTCTC	1 cut(s)	386
Eco47III	AGCGCT	1 cut(s)	147
Eco57I	CTGAAG	2 cut(s)	1013, 1316
Eco81I	CCTNAGG	1 cut(s)	72
Eco91I	GGTNACC	1 cut(s)	1319
EcoNI	CCTNNNNNAGG	1 cut(s)	422
EcoO109I	RGGNCCY	1 cut(s)	415
EcoO65I	GGTNACC	1 cut(s)	1319
EcoRI	GAATTC	1 cut(s)	1113
EcoT22I	ATGCAT	1 cut(s)	738
Esp3I	CGTCTC	1 cut(s)	245
FaeI	CATG	4 cut(s)	16, 178, 706, 1231
FalI	AAGNNNNNCTT	4 cut(s)	132, 164, 1065, 1097
FaqI	GGGAC	1 cut(s)	1127
FatI	CATG	4 cut(s)	12, 174, 702, 1227
FauI	CCCGC	1 cut(s)	799
FauNDI	CATATG	1 cut(s)	738
FbaI	TGATCA	2 cut(s)	562, 1210
FblI	GTMKAC	1 cut(s)	823
Fnu4HI	GCNGC	6 cut(s)	6, 765, 951, 1079, 1163, 1269
FokI	GGATG	2 cut(s)	678, 1031
Fsp4HI	GCNGC	6 cut(s)	6, 765, 951, 1079, 1163, 1269
FspBI	CTAG	2 cut(s)	366, 696
GlaI	GCGC	2 cut(s)	147, 1235
GluI	GCNGC	6 cut(s)	6, 765, 951, 1079, 1163, 1269
HaeII	RGCGCY	1 cut(s)	149
HaeIII	GGCC	2 cut(s)	416, 764
HapII	CCGG	1 cut(s)	357
HgaI	GACGC	1 cut(s)	737
HhaI	GCGC	2 cut(s)	148, 1236
Hin1II	CATG	4 cut(s)	16, 178, 706, 1231
Hin6I	GCGC	2 cut(s)	146, 1234
HinP1I	GCGC	2 cut(s)	146, 1234
HincII	GTYRAC	2 cut(s)	285, 581
HindII	GTYRAC	2 cut(s)	285, 581
HindIII	AAGCTT	2 cut(s)	464, 783
HinfI	GANTC	5 cut(s)	48, 266, 607, 699, 1107
HpaII	CCGG	1 cut(s)	357
HphI	GGTGA	2 cut(s)	1228, 1250
Hpy166II	GTNNAC	6 cut(s)	285, 581, 618, 824, 1101, 1219
Hpy188I	TCNGA	6 cut(s)	61, 372, 402, 871, 993, 1112
Hpy188III	TCNNGA	8 cut(s)	137, 153, 244, 451, 716, 790, 893, 920
Hpy8I	GTNNAC	6 cut(s)	285, 581, 618, 824, 1101, 1219
HpyAV	CCTTC	5 cut(s)	134, 514, 1129, 1243, 1248
HpyCH4III	ACNGT	3 cut(s)	223, 239, 320
HpyCH4IV	ACGT	1 cut(s)	84
HpyCH4V	TGCA	7 cut(s)	16, 388, 471, 599, 671, 736, 865
HpyF10VI	GCNNNNNNNGC	3 cut(s)	76, 764, 1231
HpyF3I	CTNAG	6 cut(s)	72, 192, 242, 621, 921, 1146
HpySE526I	ACGT	1 cut(s)	84
Hsp92II	CATG	4 cut(s)	16, 178, 706, 1231
HspAI	GCGC	2 cut(s)	146, 1234
Ksp22I	TGATCA	2 cut(s)	562, 1210
Kzo9I	GATC	8 cut(s)	133, 228, 562, 916, 924, 1021, 1048, 1210
LguI	GCTCTTC	1 cut(s)	60
LmnI	GCTCC	4 cut(s)	76, 143, 178, 1231
Lsp1109I	GCAGC	5 cut(s)	17, 962, 1065, 1174, 1255
LweI	GCATC	2 cut(s)	723, 852
MaeI	CTAG	2 cut(s)	366, 696
MaeII	ACGT	1 cut(s)	84
MaeIII	GTNAC	3 cut(s)	304, 1238, 1319
MalI	GATC	8 cut(s)	135, 230, 564, 918, 926, 1023, 1050, 1212
MboI	GATC	8 cut(s)	133, 228, 562, 916, 924, 1021, 1048, 1210
MfeI	CAATTG	1 cut(s)	600
MflI	RGATCY	3 cut(s)	228, 924, 1048
Mph1103I	ATGCAT	1 cut(s)	738
MroXI	GAANNNNTTC	2 cut(s)	168, 987
MseI	TTAA	5 cut(s)	204, 462, 876, 1083, 1137
MspA1I	CMGCKG	1 cut(s)	1294
MspCI	CTTAAG	1 cut(s)	461
MspI	CCGG	1 cut(s)	357
MspR9I	CCNGG	1 cut(s)	358
MunI	CAATTG	1 cut(s)	600
MvnI	CGCG	1 cut(s)	1236
MwoI	GCNNNNNNNGC	3 cut(s)	76, 764, 1231
NciI	CCSGG	1 cut(s)	358
NdeI	CATATG	1 cut(s)	738
NdeII	GATC	8 cut(s)	133, 228, 562, 916, 924, 1021, 1048, 1210
NlaIII	CATG	4 cut(s)	16, 178, 706, 1231
NlaIV	GGNNCC	2 cut(s)	180, 812
NmuCI	GTSAC	1 cut(s)	1238
NsiI	ATGCAT	1 cut(s)	738
NspI	RCATGY	1 cut(s)	16
NspV	TTCGAA	2 cut(s)	981, 1033
PciSI	GCTCTTC	1 cut(s)	60
PdmI	GAANNNNTTC	2 cut(s)	168, 987
PfeI	GAWTC	5 cut(s)	48, 266, 607, 699, 1107
PflMI	CCANNNNNTGG	2 cut(s)	887, 1228
PkrI	GCNGC	6 cut(s)	7, 766, 952, 1080, 1164, 1270
PspEI	GGTNACC	1 cut(s)	1319
PspN4I	GGNNCC	2 cut(s)	180, 812
PspPI	GGNCC	1 cut(s)	415
PsrI	GAACNNNNNNTAC	2 cut(s)	89, 121
PsuI	RGATCY	3 cut(s)	228, 924, 1048
PvuII	CAGCTG	1 cut(s)	1294
RsaI	GTAC	4 cut(s)	56, 556, 617, 1100
RsaNI	GTAC	4 cut(s)	55, 555, 616, 1099
SapI	GCTCTTC	1 cut(s)	60
SaqAI	TTAA	5 cut(s)	204, 462, 876, 1083, 1137
SatI	GCNGC	6 cut(s)	6, 765, 951, 1079, 1163, 1269
Sau3AI	GATC	8 cut(s)	133, 228, 562, 916, 924, 1021, 1048, 1210
Sau96I	GGNCC	1 cut(s)	415
ScaI	AGTACT	1 cut(s)	56
ScrFI	CCNGG	1 cut(s)	358
SfaNI	GCATC	2 cut(s)	723, 852
SfuI	TTCGAA	2 cut(s)	981, 1033
SmiI	ATTTAAAT	1 cut(s)	205
SmlI	CTYRAG	1 cut(s)	461
SmoI	CTYRAG	1 cut(s)	461
SsiI	CCGC	3 cut(s)	765, 806, 1236
SspI	AATATT	2 cut(s)	839, 1063
SspMI	CTAG	2 cut(s)	366, 696
StyD4I	CCNGG	1 cut(s)	356
SwaI	ATTTAAAT	1 cut(s)	205
TaaI	ACNGT	3 cut(s)	223, 239, 320
TaiI	ACGT	1 cut(s)	87
TaqI	TCGA	5 cut(s)	51, 981, 1033, 1197, 1247
TatI	WGTACW	2 cut(s)	54, 615
TauI	GCSGC	1 cut(s)	767
TfiI	GAWTC	5 cut(s)	48, 266, 607, 699, 1107
Tru1I	TTAA	5 cut(s)	204, 462, 876, 1083, 1137
Tru9I	TTAA	5 cut(s)	204, 462, 876, 1083, 1137
TscAI	CASTG	3 cut(s)	325, 625, 758
TseFI	GTSAC	1 cut(s)	1238
TseI	GCWGC	5 cut(s)	5, 950, 1078, 1162, 1268
Tsp45I	GTSAC	1 cut(s)	1238
TspDTI	ATGAA	4 cut(s)	258, 816, 1018, 1111
TspGWI	ACGGA	2 cut(s)	60, 325
TspRI	CASTG	3 cut(s)	325, 625, 758
Van91I	CCANNNNNTGG	2 cut(s)	887, 1228
Vha464I	CTTAAG	1 cut(s)	461
XagI	CCTNNNNNAGG	1 cut(s)	422
XapI	RAATTY	3 cut(s)	474, 987, 1113
XceI	RCATGY	1 cut(s)	16
XmiI	GTMKAC	1 cut(s)	823
XmnI	GAANNNNTTC	2 cut(s)	168, 987
XspI	CTAG	2 cut(s)	366, 696
ZrmI	AGTACT	1 cut(s)	56
Zsp2I	ATGCAT	1 cut(s)	738

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.