Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004427

Physical Location & Seq

Genomic Coordinates Reverse (-)

20429678 .. 20435271

Gene Structure 5594 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1163800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGCCTCCGATGCTGATATGGAGGATTACGGATTCGAGTATTCGGATGAGGAGCCTGAGGAACAAGATGTCGACATTGAGAACCAATATTACAACTCTAAAGGTACGACTAGGTTTTCAGTTTGGTTGAGACAGACCCAGAAGGAGCACTTAGTTCGCATGGAGCCTGAGAAGGCCGAGTGGGGATTTAAAGCTTTGAAGCAAACCGTGAAGCTCTATTATCGTCTGGGGAGGTATAAAGAAATGATGGATGCATACAGGGTGATGTTGACTTACATCAAATCAGCAGTAACAAGAAATTACAGTGAAAAGTGTATAAACAATATTATGGATTTTGTTTCTGGTTCAGCTAGTCAGAACTTTAGTCTACTGCAAGAGTTCTATCAGACCACTCTAAAGGCCCTTGAAGAGGCAAAGAATGAGAGACTCTGGTTTAAGACTAATCTTAAGCTTTGCAAAATTTGGTTTGATATGGGCGAATATGGGCGAATGAGCAAGATTTTGAAGGAACTCCATAAATCTTGTCAAAAAGAAGATGGTACCGATGACCAAAAGAAGGGAAGTCAACTTCTAGAGGTTTATGCTATTGAAATTCAAATGTACACCGAGACTAAAAATAACAAAAAGCTCAAGCAATTATACCGAAAAGCTCTTGCAATCAAGTCAGCCATACCTCATCCTAGGATCATGGGAATAATTCGAGAGTGTGGGGGTAAGATGCATATGGCAGAGCGTCAGTGGGCAGATGCAGCCACAGATTTTTTTGAAGCCTTTAAGAACTATGATGAAGCTGGGAACCAGAGGCGTATACAATGCTTAAAGTACTTGGTTCTGGCAAATATGCTGATGGAGTCGGAAGTTAATCCATTTGATGCATATCAACGGAATGAGATATTGGAGTTTGAGAAGATTCTTAAGAGTAATAGGAGGACAATTATGGATGATCCATTCATTCGAAACTATATTGAAGATCTGCTGAAGAATGTTAGGACACAAGTGCTGCTCAAACTAATGAAGCCGTACACGAGAATTCGGTTCCCTTCATATCAAAGCTTCCTCATATCACCTGTTGATGTTCAGGAACTTAATGTGCCGGAGAAAGATGTGGAGCAACTCTTGGTTTCACTTATATTGGATAACAGAATTGATGGACATATTGATCAGGTGAATCGGCTCTTAGAGCGTGGCGACAAGTCAAAGGGAATGAAGAAGTATGCTGCTGTAGATAAATGGAACACTCAGCTAAGGTCTCTTTACCAGACTATCAGTAACCGAGTATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000338 GO:0003674 GO:0003712 GO:0003714 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0019538 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0080090 GO:0140110 GO:1901564 GO:1901565 GO:1901575 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PCI

Protein Analysis

428

Amino Acids

50.57

Weight (kDa)

8.48

Isoelectric Point (pI)

38.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCI	PF01399	305 - 394	5e-17	PCI domain
COPS2_C	PF25983	402 - 426	1.6e-13	COP9 signalosome complex subunit 2, C-terminal helix

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26990
fragaria_vesca	FvH4_4g27570
malus_domestica	MD00G1108200.v1.1 MD03G1163800.v1.1 MD11G1176300.v1.1
prunus_persica	Prupe.6G156800_v2.0.a1 Prupe.6G156800_v2.0.a1
pyrus_communis	pycom11g15960 pycom11g16000
rosa_chinensis	RchiOBHm_Chr4g0435421 RchiOBHm_Chr5g0050051
rosa_laevigata	RLG00000006594 RLG00000034682
rosa_multiflora	Rmu_sc0000333.1_g000008 Rmu_sc0001940.1_g000023 Rmu_sc0009623.1_g000002 Rmu_sc0016267.1_g000001 Rmu_sc0036582.1_g000002
rosa_roxburghii	Rroxscaffold_1G00031080 Rroxscaffold_5G00376660
rosa_rugosa	Rorug04G0284900 Rorug05G0256900
rosa_samantha	Rh4AG340600 Rh4BG348900 Rh4CG363400 Rh4DG343100 Rh5AG330100 Rh5BG340800 Rh5CG367400 Rh5DG353500
rosa_wichuraiana	Rw4G029700 Rw5G031150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	539
AccB1I	GGYRCC	1 cut(s)	539
AccI	GTMKAC	3 cut(s)	72, 367, 808
AclWI	GGATC	2 cut(s)	692, 938
AcsI	RAATTY	3 cut(s)	459, 591, 1029
AcuI	CTGAAG	1 cut(s)	998
AfaI	GTAC	5 cut(s)	106, 541, 602, 824, 1022
AfiI	CCNNNNNNNGG	2 cut(s)	409, 556
AflII	CTTAAG	2 cut(s)	446, 914
AgsI	TTSAA	7 cut(s)	199, 407, 505, 590, 596, 767, 968
AjuI	GAANNNNNNNTTGG	2 cut(s)	878, 910
AluBI	AGCT	9 cut(s)	194, 214, 350, 451, 628, 650, 791, 1053, 1243
AluI	AGCT	9 cut(s)	194, 214, 350, 451, 628, 650, 791, 1053, 1243
Alw21I	GWGCWC	1 cut(s)	150
Alw26I	GTCTC	4 cut(s)	124, 418, 602, 1254
AlwI	GGATC	2 cut(s)	692, 938
AoxI	GGCC	3 cut(s)	3, 174, 399
ApeKI	GCWGC	3 cut(s)	749, 1000, 1217
ApoI	RAATTY	3 cut(s)	459, 591, 1029
Asp718I	GGTACC	1 cut(s)	539
AspA2I	CCTAGG	1 cut(s)	680
AspS9I	GGNCC	1 cut(s)	400
AsuHPI	GGTGA	3 cut(s)	274, 1056, 1177
AsuII	TTCGAA	1 cut(s)	955
AvrII	CCTAGG	1 cut(s)	680
AxyI	CCTNAGG	1 cut(s)	57
BanI	GGYRCC	1 cut(s)	539
BauI	CACGAG	1 cut(s)	1024
Bbv12I	GWGCWC	1 cut(s)	150
BbvI	GCAGC	3 cut(s)	761, 987, 1204
BccI	CCATC	4 cut(s)	241, 530, 841, 1142
BceAI	ACGGC	1 cut(s)	1003
BcgI	CGANNNNNNTGC	2 cut(s)	137, 171
BclI	TGATCA	1 cut(s)	1159
BcoDI	GTCTC	4 cut(s)	124, 418, 602, 1254
BfaI	CTAG	4 cut(s)	111, 351, 572, 681
BfmI	CTRYAG	1 cut(s)	1221
BfrI	CTTAAG	2 cut(s)	446, 914
BglII	AGATCT	1 cut(s)	970
BisI	GCNGC	3 cut(s)	750, 1001, 1218
BlnI	CCTAGG	1 cut(s)	680
BlsI	GCNGC	3 cut(s)	751, 1002, 1219
BmcAI	AGTACT	1 cut(s)	824
BmgT120I	GGNCC	1 cut(s)	400
BmiI	GGNNCC	5 cut(s)	54, 165, 541, 797, 1037
BmsI	GCATC	4 cut(s)	241, 708, 736, 862
Bpu10I	CCTNAGC	1 cut(s)	1244
Bpu14I	TTCGAA	1 cut(s)	955
BpuEI	CTTGAG	1 cut(s)	614
BsaBI	GATNNNNATC	1 cut(s)	876
BsaI	GGTCTC	1 cut(s)	1254
BsaJI	CCNNGG	1 cut(s)	680
Bsc4I	CCNNNNNNNGG	2 cut(s)	409, 556
Bse21I	CCTNAGG	1 cut(s)	57
Bse8I	GATNNNNATC	1 cut(s)	876
BseDI	CCNNGG	1 cut(s)	680
BseGI	GGATG	4 cut(s)	52, 256, 676, 946
BseJI	GATNNNNATC	1 cut(s)	876
BseLI	CCNNNNNNNGG	2 cut(s)	409, 556
BseMII	CTCAG	3 cut(s)	48, 159, 1253
BseRI	GAGGAG	1 cut(s)	65
BseXI	GCAGC	3 cut(s)	761, 987, 1204
BseYI	CCCAGC	1 cut(s)	791
BshFI	GGCC	3 cut(s)	5, 176, 401
BshNI	GGYRCC	1 cut(s)	539
BsiHKAI	GWGCWC	1 cut(s)	150
BsiSI	CCGG	1 cut(s)	1094
BslI	CCNNNNNNNGG	2 cut(s)	409, 556
BsmAI	GTCTC	4 cut(s)	124, 418, 602, 1254
BsnI	GGCC	3 cut(s)	5, 176, 401
Bso31I	GGTCTC	1 cut(s)	1254
Bsp119I	TTCGAA	1 cut(s)	955
Bsp1286I	GDGCHC	1 cut(s)	150
Bsp1407I	TGTACA	1 cut(s)	600
Bsp143I	GATC	4 cut(s)	684, 943, 970, 1159
BspANI	GGCC	3 cut(s)	5, 176, 401
BspCNI	CTCAG	3 cut(s)	49, 160, 1252
BspLI	GGNNCC	5 cut(s)	54, 165, 541, 797, 1037
BspPI	GGATC	2 cut(s)	692, 938
BspT104I	TTCGAA	1 cut(s)	955
BspT107I	GGYRCC	1 cut(s)	539
BspTI	CTTAAG	2 cut(s)	446, 914
BspTNI	GGTCTC	1 cut(s)	1254
BsrGI	TGTACA	1 cut(s)	600
BssECI	CCNNGG	1 cut(s)	680
BssMI	GATC	4 cut(s)	684, 943, 970, 1159
BssNAI	GTATAC	1 cut(s)	809
BssSI	CACGAG	1 cut(s)	1024
BssT1I	CCWWGG	1 cut(s)	680
Bst1107I	GTATAC	1 cut(s)	809
Bst2BI	CACGAG	1 cut(s)	1024
Bst4CI	ACNGT	2 cut(s)	208, 305
Bst6I	CTCTTC	1 cut(s)	402
BstAFI	CTTAAG	2 cut(s)	446, 914
BstAUI	TGTACA	1 cut(s)	600
BstBI	TTCGAA	1 cut(s)	955
BstDEI	CTNAG	6 cut(s)	57, 151, 168, 1177, 1239, 1244
BstENI	CCTNNNNNAGG	1 cut(s)	407
BstF5I	GGATG	4 cut(s)	52, 256, 676, 946
BstKTI	GATC	4 cut(s)	687, 946, 973, 1162
BstMAI	GTCTC	4 cut(s)	124, 418, 602, 1254
BstMBI	GATC	4 cut(s)	684, 943, 970, 1159
BstMWI	GCNNNNNNNGC	3 cut(s)	11, 749, 1180
BstSFI	CTRYAG	1 cut(s)	1221
BstV1I	GCAGC	3 cut(s)	761, 987, 1204
BstX2I	RGATCY	1 cut(s)	970
BstYI	RGATCY	1 cut(s)	970
BstZ17I	GTATAC	1 cut(s)	809
Bsu36I	CCTNAGG	1 cut(s)	57
BsuRI	GGCC	3 cut(s)	5, 176, 401
BtsCI	GGATG	4 cut(s)	52, 256, 676, 946
BtsIMutI	CAGTG	2 cut(s)	310, 743
Cfr13I	GGNCC	1 cut(s)	400
CseI	GACGC	1 cut(s)	722
Csp6I	GTAC	5 cut(s)	105, 540, 601, 823, 1021
CviAII	CATG	2 cut(s)	160, 688
CviQI	GTAC	5 cut(s)	105, 540, 601, 823, 1021
DdeI	CTNAG	6 cut(s)	57, 151, 168, 1177, 1239, 1244
DpnI	GATC	4 cut(s)	686, 945, 972, 1161
DpnII	GATC	4 cut(s)	684, 943, 970, 1159
DraI	TTTAAA	1 cut(s)	190
Eam1104I	CTCTTC	1 cut(s)	402
EarI	CTCTTC	1 cut(s)	402
Eco130I	CCWWGG	1 cut(s)	680
Eco31I	GGTCTC	1 cut(s)	1254
Eco57I	CTGAAG	1 cut(s)	998
Eco81I	CCTNAGG	1 cut(s)	57
EcoNI	CCTNNNNNAGG	1 cut(s)	407
EcoO109I	RGGNCCY	1 cut(s)	400
EcoRI	GAATTC	1 cut(s)	1029
EcoT14I	CCWWGG	1 cut(s)	680
EcoT22I	ATGCAT	3 cut(s)	256, 723, 877
ErhI	CCWWGG	1 cut(s)	680
FaeI	CATG	2 cut(s)	163, 691
FalI	AAGNNNNNCTT	2 cut(s)	134, 166
FatI	CATG	2 cut(s)	159, 687
FauNDI	CATATG	1 cut(s)	723
FbaI	TGATCA	1 cut(s)	1159
FblI	GTMKAC	3 cut(s)	72, 367, 808
Fnu4HI	GCNGC	3 cut(s)	750, 1001, 1218
FokI	GGATG	4 cut(s)	59, 263, 663, 953
Fsp4HI	GCNGC	3 cut(s)	750, 1001, 1218
FspBI	CTAG	4 cut(s)	111, 351, 572, 681
GluI	GCNGC	3 cut(s)	750, 1001, 1218
GsaI	CCCAGC	1 cut(s)	795
HaeIII	GGCC	3 cut(s)	5, 176, 401
HapII	CCGG	1 cut(s)	1094
HgaI	GACGC	1 cut(s)	722
Hin1II	CATG	2 cut(s)	163, 691
HincII	GTYRAC	3 cut(s)	73, 270, 566
HindII	GTYRAC	3 cut(s)	73, 270, 566
HindIII	AAGCTT	3 cut(s)	192, 449, 1051
HinfI	GANTC	5 cut(s)	33, 426, 851, 910, 1168
HpaII	CCGG	1 cut(s)	1094
HphI	GGTGA	3 cut(s)	274, 1056, 1177
Hpy166II	GTNNAC	7 cut(s)	73, 270, 368, 566, 603, 809, 1023
Hpy188I	TCNGA	5 cut(s)	10, 46, 357, 387, 856
Hpy188III	TCNNGA	3 cut(s)	572, 701, 1079
Hpy8I	GTNNAC	7 cut(s)	73, 270, 368, 566, 603, 809, 1023
HpyAV	CCTTC	5 cut(s)	136, 166, 499, 550, 1050
HpyCH4III	ACNGT	2 cut(s)	208, 305
HpyCH4V	TGCA	7 cut(s)	254, 373, 456, 656, 721, 749, 875
HpyF10VI	GCNNNNNNNGC	3 cut(s)	11, 749, 1180
HpyF3I	CTNAG	6 cut(s)	57, 151, 168, 1177, 1239, 1244
Hsp92II	CATG	2 cut(s)	163, 691
KpnI	GGTACC	1 cut(s)	543
Ksp22I	TGATCA	1 cut(s)	1159
Kzo9I	GATC	4 cut(s)	684, 943, 970, 1159
LmnI	GCTCC	4 cut(s)	52, 145, 163, 1108
Lsp1109I	GCAGC	3 cut(s)	761, 987, 1204
LweI	GCATC	4 cut(s)	241, 708, 736, 862
MaeI	CTAG	4 cut(s)	111, 351, 572, 681
MaeIII	GTNAC	2 cut(s)	289, 1268
MalI	GATC	4 cut(s)	686, 945, 972, 1161
MboI	GATC	4 cut(s)	684, 943, 970, 1159
MboII	GAAGA	6 cut(s)	419, 545, 919, 980, 991, 1219
MflI	RGATCY	1 cut(s)	970
MhlI	GDGCHC	1 cut(s)	150
MluCI	AATT	8 cut(s)	298, 459, 591, 635, 696, 933, 1029, 1143
MlyI	GAGTC	2 cut(s)	420, 860
MmeI	TCCRAC	1 cut(s)	834
Mph1103I	ATGCAT	3 cut(s)	256, 723, 877
MseI	TTAA	8 cut(s)	189, 435, 447, 774, 818, 861, 915, 1086
MspCI	CTTAAG	2 cut(s)	446, 914
MspI	CCGG	1 cut(s)	1094
MwoI	GCNNNNNNNGC	3 cut(s)	11, 749, 1180
NdeI	CATATG	1 cut(s)	723
NdeII	GATC	4 cut(s)	684, 943, 970, 1159
NlaIII	CATG	2 cut(s)	163, 691
NlaIV	GGNNCC	5 cut(s)	54, 165, 541, 797, 1037
NmeAIII	GCCGAG	1 cut(s)	202
NsiI	ATGCAT	3 cut(s)	256, 723, 877
NspV	TTCGAA	1 cut(s)	955
PfeI	GAWTC	3 cut(s)	33, 910, 1168
PkrI	GCNGC	3 cut(s)	751, 1002, 1219
PleI	GAGTC	2 cut(s)	420, 859
PpsI	GAGTC	2 cut(s)	420, 859
PspFI	CCCAGC	1 cut(s)	791
PspN4I	GGNNCC	5 cut(s)	54, 165, 541, 797, 1037
PspPI	GGNCC	1 cut(s)	400
PsrI	GAACNNNNNNTAC	2 cut(s)	74, 106
PsuI	RGATCY	1 cut(s)	970
RsaI	GTAC	5 cut(s)	106, 541, 602, 824, 1022
RsaNI	GTAC	5 cut(s)	105, 540, 601, 823, 1021
SalI	GTCGAC	1 cut(s)	71
SaqAI	TTAA	8 cut(s)	189, 435, 447, 774, 818, 861, 915, 1086
SatI	GCNGC	3 cut(s)	750, 1001, 1218
Sau3AI	GATC	4 cut(s)	684, 943, 970, 1159
Sau96I	GGNCC	1 cut(s)	400
ScaI	AGTACT	1 cut(s)	824
SchI	GAGTC	2 cut(s)	420, 860
SduI	GDGCHC	1 cut(s)	150
SfaNI	GCATC	4 cut(s)	241, 708, 736, 862
SfcI	CTRYAG	1 cut(s)	1221
SfuI	TTCGAA	1 cut(s)	955
SmlI	CTYRAG	3 cut(s)	446, 629, 914
SmoI	CTYRAG	3 cut(s)	446, 629, 914
Sse9I	AATT	8 cut(s)	298, 459, 591, 635, 696, 933, 1029, 1143
SspI	AATATT	2 cut(s)	89, 325
SspMI	CTAG	4 cut(s)	111, 351, 572, 681
StyI	CCWWGG	1 cut(s)	680
TaaI	ACNGT	2 cut(s)	208, 305
TaqI	TCGA	4 cut(s)	36, 72, 700, 955
TasI	AATT	8 cut(s)	298, 459, 591, 635, 696, 933, 1029, 1143
TatI	WGTACW	2 cut(s)	600, 822
TfiI	GAWTC	3 cut(s)	33, 910, 1168
Tru1I	TTAA	8 cut(s)	189, 435, 447, 774, 818, 861, 915, 1086
Tru9I	TTAA	8 cut(s)	189, 435, 447, 774, 818, 861, 915, 1086
TscAI	CASTG	2 cut(s)	310, 743
TseI	GCWGC	3 cut(s)	749, 1000, 1217
TspDTI	ATGAA	5 cut(s)	801, 940, 1028, 1032, 1220
TspGWI	ACGGA	2 cut(s)	45, 898
TspRI	CASTG	2 cut(s)	310, 743
Vha464I	CTTAAG	2 cut(s)	446, 914
XagI	CCTNNNNNAGG	1 cut(s)	407
XapI	RAATTY	3 cut(s)	459, 591, 1029
XbaI	TCTAGA	1 cut(s)	571
XmaJI	CCTAGG	1 cut(s)	680
XmiI	GTMKAC	3 cut(s)	72, 367, 808
XspI	CTAG	4 cut(s)	111, 351, 572, 681
ZrmI	AGTACT	1 cut(s)	824
Zsp2I	ATGCAT	3 cut(s)	256, 723, 877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.