MD00G1113300.v1.1

Basic leucine zipper and W2 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006554
Physical Location & Seq
Forward (+)
23948848 .. 23949445
598 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1113300.v1.1.491

Sequence Viewer

Length: 447 bp
ATGCGACGATTCCTTCAATCGTTAGAGCTTTTTGAGGAAAATGAAAGGAAGAAGCTGGCTATTTTCACAGCACTTGCATTCTCTCAGAAGCTGTCAGGACTTCCACCAGAAACGGTGTTCCAGCCAATGCTCAAGGATAATCTTGTTGGCAAAGGGCTAGTTCTATCTCTTGATAATTTGATTTCCATTCTGAAGCGGGGTAAAGTGGAGGACAATCTTTTGGAATTCTTCCCTTCTGCAAAGAGAACCGAAGAGAGTTTCTCTGAGCATTTCACAAAGGAATGGCTAGTTGCCTTAGTTGAGTACAACGAGAAGAAAATTTTCGAAGTGAAGCTTAAGGAAATGAAATCTGCTTTAACAACCCAGATAACAGAGGAAACCGATATGTCTGAAGTCATTGAGACTGTGAAGCAGCGTGTTAAAGATGCCAAATTGCCCGATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
W2

Protein Analysis

149

Amino Acids

17.11

Weight (kDa)

5.63

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_5MP1_2 PF25504 1 - 111 6.3e-26 5MP1/2 HEAT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 196
AcsI RAATTY 2 cut(s) 224, 318
AcuI CTGAAG 2 cut(s) 212, 411
AfaI GTAC 1 cut(s) 305
AflII CTTAAG 1 cut(s) 335
AgsI TTSAA 1 cut(s) 17
AluBI AGCT 4 cut(s) 28, 55, 91, 334
AluI AGCT 4 cut(s) 28, 55, 91, 334
Alw26I GTCTC 1 cut(s) 395
AlwNI CAGNNNCTG 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 412
ApoI RAATTY 2 cut(s) 224, 318
Asp700I GAANNNNTTC 1 cut(s) 320
AsuII TTCGAA 1 cut(s) 324
BbvI GCAGC 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 2 cut(s) 158, 287
BfrI CTTAAG 1 cut(s) 335
BisI GCNGC 1 cut(s) 413
BlsI GCNGC 1 cut(s) 414
BmsI GCATC 1 cut(s) 415
BplI GAGNNNNNCTC 2 cut(s) 245, 277
Bpu14I TTCGAA 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 116
BseMII CTCAG 2 cut(s) 98, 255
BseXI GCAGC 1 cut(s) 424
BsmAI GTCTC 1 cut(s) 395
BsmI GAATGC 1 cut(s) 77
Bsp119I TTCGAA 1 cut(s) 324
BspACI CCGC 1 cut(s) 196
BspCNI CTCAG 2 cut(s) 97, 256
BspT104I TTCGAA 1 cut(s) 324
BspTI CTTAAG 1 cut(s) 335
Bst4CI ACNGT 2 cut(s) 115, 406
Bst6I CTCTTC 1 cut(s) 246
BstAFI CTTAAG 1 cut(s) 335
BstBI TTCGAA 1 cut(s) 324
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 3 cut(s) 84, 264, 295
BstMAI GTCTC 1 cut(s) 395
BstV1I GCAGC 1 cut(s) 424
Cac8I GCNNGC 1 cut(s) 57
CaiI CAGNNNCTG 1 cut(s) 91
Csp6I GTAC 1 cut(s) 304
CviJI RGCY 8 cut(s) 28, 55, 59, 91, 124, 157, 286, 334
CviKI_1 RGCY 8 cut(s) 28, 55, 59, 91, 124, 157, 286, 334
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 3 cut(s) 84, 264, 295
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco57I CTGAAG 2 cut(s) 212, 411
EcoRI GAATTC 1 cut(s) 224
FaiI YATR 1 cut(s) 386
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FauI CCCGC 1 cut(s) 189
Fnu4HI GCNGC 1 cut(s) 413
Fsp4HI GCNGC 1 cut(s) 413
FspBI CTAG 2 cut(s) 158, 287
GluI GCNGC 1 cut(s) 413
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 1 cut(s) 9
Hpy188I TCNGA 4 cut(s) 87, 192, 265, 391
Hpy188III TCNNGA 2 cut(s) 96, 170
Hpy99I CGWCG 1 cut(s) 9
HpyAV CCTTC 2 cut(s) 23, 243
HpyCH4III ACNGT 2 cut(s) 115, 406
HpyCH4V TGCA 2 cut(s) 77, 239
HpyF3I CTNAG 3 cut(s) 84, 264, 295
LpnPI CCDG 5 cut(s) 41, 81, 120, 134, 377
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 1 cut(s) 415
MaeI CTAG 2 cut(s) 158, 287
MboII GAAGA 4 cut(s) 61, 220, 263, 325
MluCI AATT 4 cut(s) 175, 224, 318, 431
MnlI CCTC 3 cut(s) 28, 202, 367
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 3 cut(s) 336, 356, 420
MspCI CTTAAG 1 cut(s) 335
Mva1269I GAATGC 1 cut(s) 77
NspV TTCGAA 1 cut(s) 324
PctI GAATGC 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 1 cut(s) 9
PkrI GCNGC 1 cut(s) 414
PstNI CAGNNNCTG 1 cut(s) 91
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
SaqAI TTAA 3 cut(s) 336, 356, 420
SatI GCNGC 1 cut(s) 413
SetI ASST 4 cut(s) 30, 57, 93, 336
SfaNI GCATC 1 cut(s) 415
SfuI TTCGAA 1 cut(s) 324
SmlI CTYRAG 2 cut(s) 131, 335
SmoI CTYRAG 2 cut(s) 131, 335
Sse9I AATT 4 cut(s) 175, 224, 318, 431
SsiI CCGC 1 cut(s) 196
SspMI CTAG 2 cut(s) 158, 287
TaaI ACNGT 2 cut(s) 115, 406
TaqI TCGA 1 cut(s) 324
TasI AATT 4 cut(s) 175, 224, 318, 431
TatI WGTACW 1 cut(s) 303
TfiI GAWTC 1 cut(s) 9
Tru1I TTAA 3 cut(s) 336, 356, 420
Tru9I TTAA 3 cut(s) 336, 356, 420
TseI GCWGC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 57, 359
Vha464I CTTAAG 1 cut(s) 335
XapI RAATTY 2 cut(s) 224, 318
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 2 cut(s) 158, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.