Rh3DG005600

Basic leucine zipper and W2 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0006554
Physical Location & Seq
Reverse (-)
399125 .. 402383
3259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG005600.1

Sequence Viewer

Length: 1236 bp
ATGAGCTCGAAGGAGAGACCCACTCTCGGTGGCACGCGGATTAAGACCCGCAAACGGAATATTGCAGCGCCGCTGGACCCTGCAGCTTTTGCGGATGCAGTGGTCCAGATTTATCTGGATAATGCTGGTGATCTGGAACTTATTGCCAAGAGCATTGAATCTTCAGACCTTAACTTCTCAAGATACGGTGACACCTTTTTTGAGGTTGTTTTCACTGGGGGCCGTACACAACCTGGAACAACAAAACCTGATGAGGGGGAGCGCCACCCTTACTCTGTACTAGAGTCTGAGCCTAGACGTGAAGTCATTATACCATCTGTTATCTACATACAAAAGATTTTGAGGCGGAGGCCATTTCTGATAAAGAATCTCGAAAATGTCATGCGACGTTTCCTTCAGTCATTGGAGCTTTTTGAGGAAAATGAAAGGAAGAAGCTGGCAATTTTCACAGCACTTGCATTCTCTCAGAAGCTATCAGGACTTCCACCGGAAACGGTGTTCCAGCCAATGCTCAAGGATAATCTTGTTGGCAAAGGGCTAGTTCTATCGTTTATAACTGAGTTCTTCAAGGAGTATCTGATTGACAATAGCCTTGATGATTTGATTTCGATTCTGAAACGGGGTAAAGTGGAGGATAATCTTCTGGACTTCTTCCCAGCCGCAAAGCGAACTGAGGAGAGTTTCTCTGAGCATTTCACCAAGGAAGGGCTAGTGTCCTTGGTTGAATACAATGAAAAGAAGATTTTTGAGGTGAAGCTTAAAGATATGAAATCTGCTTTGACAACCCAGATAACAGAGGAAACTGATATATCTGAAGTCATAGAAACTGTGAAGCAGCGCGTTAAAGATGCCAAATTGCCAGATGTTGAGGTTGTGCGTATCCTGTGGGATGTTATTATGGATGCTGTGCAGTGGTCTGGTAAGAATCAGCAACAGAATGCCAATGCAGCTCTTCGCCAGGTGAAAACATGGGCACAACTGTTGAATGCCTTCTGCACCAATGGGAAGCTTGAGCTGGAGCTGATATACAAAGTTCAGATGCAGTGCTATGAGGATGCTAAGCTGATGAAGCTGTTCTCTGAAATTGTTAGGTCGCTGTATGAGCAGGATGTGCTTGCAGAAGACACTATCCTCCATTGGTTCCGCAAAGGAACAAATCCCAAGGGCAGGCAAACATTTGTGAAAGCTCTGGAGCCTTTTGTGAAGTGGCTGGAGGAGGCAGAAGAAGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
W2

Protein Analysis

411

Amino Acids

47.32

Weight (kDa)

5.47

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_5MP1_2 PF25504 14 - 252 6.9e-82 5MP1/2 HEAT domain
W2 PF02020 338 - 411 1.3e-19 eIF4-gamma/eIF5/eIF2-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AccII CGCG 2 cut(s) 37, 840
AciI CCGC 7 cut(s) 37, 49, 71, 92, 346, 660, 1144
AcuI CTGAAG 3 cut(s) 147, 380, 834
AfaI GTAC 2 cut(s) 226, 279
AfiI CCNNNNNNNGG 5 cut(s) 26, 54, 254, 705, 1167
AgsI TTSAA 4 cut(s) 158, 568, 725, 985
AhdI GACNNNNNGTC 1 cut(s) 302
AjiI CACGTC 1 cut(s) 299
AjnI CCWGG 2 cut(s) 232, 957
AloI GAACNNNNNNTCC 2 cut(s) 482, 514
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 10
AoxI GGCC 2 cut(s) 220, 350
ApeKI GCWGC 4 cut(s) 65, 83, 835, 947
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
Asp700I GAANNNNTTC 2 cut(s) 989, 1073
AspLEI GCGC 3 cut(s) 70, 264, 840
AspS9I GGNCC 3 cut(s) 76, 103, 220
AsuHPI GGTGA 5 cut(s) 140, 200, 688, 763, 973
AvaII GGWCC 2 cut(s) 76, 103
BaeGI GKGCMC 1 cut(s) 976
BanII GRGCYC 1 cut(s) 8
BarI GAAGNNNNNNTAC 2 cut(s) 294, 326
BbsI GAAGAC 1 cut(s) 1128
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 77, 95, 847, 959
BccI CCATC 1 cut(s) 322
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 2 cut(s) 234, 959
BciVI GTATCC 1 cut(s) 890
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 4 cut(s) 281, 294, 539, 710
BfmI CTRYAG 1 cut(s) 81
BfoI RGCGCY 2 cut(s) 71, 265
BfuI GTATCC 1 cut(s) 890
BisI GCNGC 6 cut(s) 66, 71, 84, 660, 836, 948
BlpI GCTNAGC 1 cut(s) 1059
BlsI GCNGC 6 cut(s) 67, 72, 85, 661, 837, 949
Bme1390I CCNGG 2 cut(s) 234, 959
Bme18I GGWCC 2 cut(s) 76, 103
BmeRI GACNNNNNGTC 1 cut(s) 302
BmgBI CACGTC 1 cut(s) 299
BmgT120I GGNCC 3 cut(s) 76, 103, 220
BmiI GGNNCC 4 cut(s) 78, 221, 1142, 1194
BmrFI CCNGG 2 cut(s) 234, 959
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 5 cut(s) 85, 838, 892, 1029, 1045
BmuI ACTGGG 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 1128
BplI GAGNNNNNCTC 4 cut(s) 7, 39, 668, 700
BpmI CTGGAG 3 cut(s) 1037, 1211, 1232
Bpu1102I GCTNAGC 1 cut(s) 1059
BpuEI CTTGAG 3 cut(s) 163, 497, 1031
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 3 cut(s) 699, 717, 1161
BsaWI WCCGGW 1 cut(s) 487
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 54, 254, 705, 1167
Bse1I ACTGG 1 cut(s) 220
BseBI CCWGG 2 cut(s) 234, 959
BseDI CCNNGG 3 cut(s) 699, 717, 1161
BseGI GGATG 5 cut(s) 100, 895, 907, 1060, 1114
BseLI CCNNNNNNNGG 5 cut(s) 26, 54, 254, 705, 1167
BseMII CTCAG 5 cut(s) 279, 479, 549, 663, 678
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 2 cut(s) 689, 1229
BseSI GKGCMC 1 cut(s) 976
BseXI GCAGC 4 cut(s) 77, 95, 847, 959
BseYI CCCAGC 1 cut(s) 655
BsgI GTGCAG 2 cut(s) 929, 979
Bsh1236I CGCG 2 cut(s) 37, 840
BshFI GGCC 2 cut(s) 222, 352
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 1 cut(s) 488
BslI CCNNNNNNNGG 5 cut(s) 26, 54, 254, 705, 1167
BsmAI GTCTC 1 cut(s) 10
BsmI GAATGC 3 cut(s) 458, 943, 991
BsnI GGCC 2 cut(s) 222, 352
Bso31I GGTCTC 1 cut(s) 10
Bsp1286I GDGCHC 2 cut(s) 8, 976
Bsp143I GATC 1 cut(s) 130
Bsp1720I GCTNAGC 1 cut(s) 1059
BspACI CCGC 7 cut(s) 37, 49, 71, 92, 346, 660, 1144
BspANI GGCC 2 cut(s) 222, 352
BspCNI CTCAG 5 cut(s) 280, 478, 550, 664, 679
BspFNI CGCG 2 cut(s) 37, 840
BspLI GGNNCC 4 cut(s) 78, 221, 1142, 1194
BspMAI CTGCAG 1 cut(s) 85
BspQI GCTCTTC 1 cut(s) 957
BspTNI GGTCTC 1 cut(s) 10
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 3 cut(s) 699, 717, 1161
BssMI GATC 1 cut(s) 130
BssT1I CCWWGG 3 cut(s) 699, 717, 1161
Bst2UI CCWGG 2 cut(s) 234, 959
Bst4CI ACNGT 4 cut(s) 188, 496, 829, 981
Bst6I CTCTTC 2 cut(s) 957, 1221
BstAPI GCANNNNNTGC 2 cut(s) 89, 1111
BstC8I GCNNGC 4 cut(s) 35, 438, 1116, 1169
BstDEI CTNAG 6 cut(s) 288, 465, 558, 672, 687, 1059
BstF5I GGATG 5 cut(s) 100, 895, 907, 1060, 1114
BstFNI CGCG 2 cut(s) 37, 840
BstH2I RGCGCY 2 cut(s) 71, 265
BstHHI GCGC 3 cut(s) 70, 264, 840
BstKTI GATC 1 cut(s) 133
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 5 cut(s) 89, 947, 1069, 1102, 1111
BstNI CCWGG 2 cut(s) 234, 959
BstSCI CCNGG 2 cut(s) 232, 957
BstSFI CTRYAG 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 976
BstUI CGCG 2 cut(s) 37, 840
BstV1I GCAGC 4 cut(s) 77, 95, 847, 959
BstV2I GAAGAC 1 cut(s) 1128
BsuI GTATCC 1 cut(s) 890
BsuRI GGCC 2 cut(s) 222, 352
BtrI CACGTC 1 cut(s) 299
BtsCI GGATG 5 cut(s) 100, 895, 907, 1060, 1114
BtsI GCAGTG 3 cut(s) 105, 917, 1049
BtsIMutI CAGTG 4 cut(s) 105, 213, 917, 1049
Cac8I GCNNGC 4 cut(s) 35, 438, 1116, 1169
CfoI GCGC 3 cut(s) 70, 264, 840
Cfr13I GGNCC 3 cut(s) 76, 103, 220
Csp6I GTAC 2 cut(s) 225, 278
CviAII CATG 2 cut(s) 382, 969
CviQI GTAC 2 cut(s) 225, 278
DdeI CTNAG 6 cut(s) 288, 465, 558, 672, 687, 1059
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
DriI GACNNNNNGTC 1 cut(s) 302
Eam1104I CTCTTC 2 cut(s) 957, 1221
Eam1105I GACNNNNNGTC 1 cut(s) 302
EarI CTCTTC 2 cut(s) 957, 1221
EciI GGCGGA 1 cut(s) 361
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 699, 717, 1161
Eco24I GRGCYC 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 2 cut(s) 76, 103
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 3 cut(s) 147, 380, 834
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 2 cut(s) 232, 957
EcoT14I CCWWGG 3 cut(s) 699, 717, 1161
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 3 cut(s) 699, 717, 1161
FaeI CATG 2 cut(s) 385, 972
FatI CATG 2 cut(s) 381, 968
FauI CCCGC 1 cut(s) 56
Fnu4HI GCNGC 6 cut(s) 66, 71, 84, 660, 836, 948
FokI GGATG 5 cut(s) 107, 902, 914, 1067, 1121
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 6 cut(s) 66, 71, 84, 660, 836, 948
FspBI CTAG 4 cut(s) 281, 294, 539, 710
GlaI GCGC 3 cut(s) 69, 263, 839
GluI GCNGC 6 cut(s) 66, 71, 84, 660, 836, 948
GsaI CCCAGC 1 cut(s) 659
GsuI CTGGAG 3 cut(s) 1037, 1211, 1232
HaeII RGCGCY 2 cut(s) 71, 265
HaeIII GGCC 2 cut(s) 222, 352
HapII CCGG 1 cut(s) 488
HhaI GCGC 3 cut(s) 70, 264, 840
Hin1II CATG 2 cut(s) 385, 972
Hin6I GCGC 3 cut(s) 68, 262, 838
HinP1I GCGC 3 cut(s) 68, 262, 838
HindIII AAGCTT 2 cut(s) 755, 1007
HinfI GANTC 5 cut(s) 158, 284, 367, 610, 925
HpaII CCGG 1 cut(s) 488
HphI GGTGA 5 cut(s) 140, 200, 688, 763, 973
Hpy166II GTNNAC 1 cut(s) 227
Hpy188III TCNNGA 8 cut(s) 106, 116, 134, 180, 371, 477, 644, 1190
Hpy8I GTNNAC 1 cut(s) 227
Hpy99I CGWCG 1 cut(s) 390
HpyAV CCTTC 4 cut(s) 4, 404, 698, 1000
HpyCH4III ACNGT 4 cut(s) 188, 496, 829, 981
HpyCH4IV ACGT 2 cut(s) 298, 388
HpyCH4V TGCA 9 cut(s) 65, 83, 98, 458, 910, 947, 996, 1042, 1118
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 947, 1069, 1102, 1111
HpyF3I CTNAG 6 cut(s) 288, 465, 558, 672, 687, 1059
HpySE526I ACGT 2 cut(s) 298, 388
Hsp92II CATG 2 cut(s) 385, 972
HspAI GCGC 3 cut(s) 68, 262, 838
Kzo9I GATC 1 cut(s) 130
LguI GCTCTTC 1 cut(s) 957
LmnI GCTCC 4 cut(s) 259, 406, 1018, 1192
Lsp1109I GCAGC 4 cut(s) 77, 95, 847, 959
LweI GCATC 5 cut(s) 85, 838, 892, 1029, 1045
MaeI CTAG 4 cut(s) 281, 294, 539, 710
MaeII ACGT 2 cut(s) 298, 388
MaeIII GTNAC 1 cut(s) 188
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MboII GAAGA 9 cut(s) 153, 442, 556, 632, 643, 751, 944, 1133, 1235
MhlI GDGCHC 2 cut(s) 8, 976
MluCI AATT 3 cut(s) 441, 854, 1083
MlyI GAGTC 1 cut(s) 293
MroXI GAANNNNTTC 2 cut(s) 989, 1073
MseI TTAA 4 cut(s) 42, 171, 759, 843
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 1 cut(s) 488
MspR9I CCNGG 2 cut(s) 234, 959
Mva1269I GAATGC 3 cut(s) 458, 943, 991
MvaI CCWGG 2 cut(s) 234, 959
MvnI CGCG 2 cut(s) 37, 840
MwoI GCNNNNNNNGC 5 cut(s) 89, 947, 1069, 1102, 1111
NdeII GATC 1 cut(s) 130
NlaIII CATG 2 cut(s) 385, 972
NlaIV GGNNCC 4 cut(s) 78, 221, 1142, 1194
NmuCI GTSAC 1 cut(s) 188
PciSI GCTCTTC 1 cut(s) 957
PctI GAATGC 3 cut(s) 458, 943, 991
PdmI GAANNNNTTC 2 cut(s) 989, 1073
PfeI GAWTC 4 cut(s) 158, 367, 610, 925
PkrI GCNGC 6 cut(s) 67, 72, 85, 661, 837, 949
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
PsiI TTATAA 1 cut(s) 554
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 2 cut(s) 232, 957
PspFI CCCAGC 1 cut(s) 655
PspGI CCWGG 2 cut(s) 232, 957
PspN4I GGNNCC 4 cut(s) 78, 221, 1142, 1194
PspPI GGNCC 3 cut(s) 76, 103, 220
PstI CTGCAG 1 cut(s) 85
RsaI GTAC 2 cut(s) 226, 279
RsaNI GTAC 2 cut(s) 225, 278
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 957
SaqAI TTAA 4 cut(s) 42, 171, 759, 843
SatI GCNGC 6 cut(s) 66, 71, 84, 660, 836, 948
Sau3AI GATC 1 cut(s) 130
Sau96I GGNCC 3 cut(s) 76, 103, 220
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 234, 959
SduI GDGCHC 2 cut(s) 8, 976
SfaNI GCATC 5 cut(s) 85, 838, 892, 1029, 1045
SfcI CTRYAG 1 cut(s) 81
SinI GGWCC 2 cut(s) 76, 103
SmlI CTYRAG 3 cut(s) 178, 512, 1010
SmoI CTYRAG 3 cut(s) 178, 512, 1010
Sse9I AATT 3 cut(s) 441, 854, 1083
SsiI CCGC 7 cut(s) 37, 49, 71, 92, 346, 660, 1144
SspI AATATT 1 cut(s) 61
SspMI CTAG 4 cut(s) 281, 294, 539, 710
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 232, 957
StyI CCWWGG 3 cut(s) 699, 717, 1161
TaaI ACNGT 4 cut(s) 188, 496, 829, 981
TaiI ACGT 2 cut(s) 301, 391
TaqI TCGA 3 cut(s) 8, 372, 608
TasI AATT 3 cut(s) 441, 854, 1083
TatI WGTACW 1 cut(s) 277
TauI GCSGC 2 cut(s) 73, 662
TfiI GAWTC 4 cut(s) 158, 367, 610, 925
Tru1I TTAA 4 cut(s) 42, 171, 759, 843
Tru9I TTAA 4 cut(s) 42, 171, 759, 843
TscAI CASTG 4 cut(s) 105, 220, 917, 1049
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 4 cut(s) 65, 83, 835, 947
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 4 cut(s) 438, 747, 782, 1082
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 4 cut(s) 105, 220, 917, 1049
VpaK11BI GGWCC 2 cut(s) 76, 103
XmnI GAANNNNTTC 2 cut(s) 989, 1073
XspI CTAG 4 cut(s) 281, 294, 539, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.