Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006554

Physical Location & Seq

Genomic Coordinates Forward (+)

32096640 .. 32100670

Gene Structure 4031 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1245300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1401 bp

ATGAGGTTGGAATTTTTGGATGTTAATTGTGGTGCAATTGTAAAGCTTTTCCTTTTCTTGGGTGGGGGTCAGCTCGAAGGAGAGACCCACTCTCGGGTAAGTCTTCGTCATATTCTGTCTCATCGGATTTACCCTCTGTGTTTTTTGGTTATGCTTTCATGTTTTTCTTACGTTTCAGAGTCAAGTGGCACGCGGATTAAGACCCGCAAACGGAATATTGCAGCGCCGCTGGACCCTGCAGCTTTTGCGGATGCAGTGGTCCAGATTTATCTGGATAATGCTGGTGATCTGGAACTTATTGCCAAGAGCATTGAATCTTCAGACCTTAACTTTTCAAGATACGGTGACACCTTTTTTGAGGCAAGCTTCGTTGTTTTCACTGGAGGCCGTACACAACCTGGAACAACAAAATCTGATGAGGGTGAGCGCCACCCTTACTCTGTACTAGAGTCTGAGCCTACACGCGAACTCATCTTGCCATCGGTTATCTACATTCAAAAAATTTTGAGGCGGAGGCCATTTCTTATTAAGAATCTCGAAAATGTTATGCGACGATTCCTTCAGTCATTGGAGCTTTTTGAGGAAAATGAAAGGAAGAAGCTGGCTATTTTCACAGCACTTGCATTCTCTCAGAAGCTGTCAGGACTTCCACCGGAAACGGTGTTCCAGCCAATGCTCAAGGATAATCTTGTTGGCAAAGGGCTAGTCCTATCATTCATTACTGACTTCTTCAAGGAGTATTTGATTGATAATAGCCTTGATGATTTGATCTCCATTCTGAAGCGGGGTAAAGTGGAGGACAATCTTTTGGAATTCTTCCCATCCACAAAGAGAACTGATGAGAGTTTCTCTGAGCATTTCACTAAGGAAGGGCTAGTCGCCTTAGTTGAATACAACGAGAAGAAAATTTTCGATGTGAAGCTCAAGGAAATGAAATCTGCTTTAACAACCCAGATAACAGAGGAAACAGATATGTCTGAAGTCATTGAGACTGTGAAGCAGCGTGTTAAAGACGCCAAATTGCCTGATGTTGAAGTTGTGCGGATTTTGTGGGATGTTATTATGGATGCTGTGCAGTGGTCGGGCAAGAACCAGCAGCAGAACGCCAATGCAGCTCTTCGCCAGGTGAAAACATGGGCGAAACTGTTGAACACCTTCTGCACGAATGGGAAGCTTGAGCTGGAACTGATATACAAGGTTCAGATGCAGTGCTATGAGGATGCTAAGCTGATGAAGCTGTTCCCTGAAATTGTGAGATCCCTCTATGAACAGGATGTGCTTGCAGAAGACACCATTCTCCATTGGTTCCGCAAAGGAACAAACCCCAAGGGCAGGCAAACTTTTGTGAAGGCCCTGGAGCCATTCGTGAACTGGCTGGAGGAGGCAGAAGAGGAGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Protein Analysis

467

Amino Acids

53.55

Weight (kDa)

5.51

Isoelectric Point (pI)

41.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HEAT_5MP1_2	PF25504	66 - 307	1.3e-79	5MP1/2 HEAT domain
W2	PF02020	393 - 466	4.6e-20	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006554)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65220 AT5G36230 AT5G36230
fragaria_vesca	FvH4_1g28180 FvH4_6g00450 FvH4_6g00450 FvH4_6g00450
malus_domestica	MD00G1113300.v1.1 MD04G1245300.v1.1 MD12G1086500.v1.1 MD13G1060900.v1.1
prunus_persica	Prupe.6G361900_v2.0.a1
pyrus_communis	pycom04g21600 pycom06g10770 pycom12g23900
rosa_chinensis	RchiOBHm_Chr3g0447821
rosa_laevigata	RLG00000018976 RLG00000025968
rosa_multiflora	Rmu_co8456651.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426020
rosa_rugosa	Rorug02G0606000
rosa_samantha	Rh3AG005500 Rh3BG005100 Rh3CG005400 Rh3DG005600
rosa_wichuraiana	Rw3G000410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	193, 465
AciI	CCGC	8 cut(s)	193, 205, 227, 248, 511, 784, 1042, 1309
AclWI	GGATC	1 cut(s)	1251
AcsI	RAATTY	4 cut(s)	11, 501, 812, 906
AcuI	CTGAAG	4 cut(s)	303, 545, 800, 999
AcyI	GRCGYC	1 cut(s)	1014
AfaI	GTAC	2 cut(s)	391, 444
AfiI	CCNNNNNNNGG	5 cut(s)	58, 93, 94, 210, 1332
AgsI	TTSAA	7 cut(s)	314, 336, 497, 733, 890, 1034, 1150
AjnI	CCWGG	3 cut(s)	397, 1122, 1353
AloI	GAACNNNNNNTCC	2 cut(s)	647, 679
Alw26I	GTCTC	3 cut(s)	77, 123, 983
AlwI	GGATC	1 cut(s)	1251
AlwNI	CAGNNNCTG	1 cut(s)	637
Ama87I	CYCGRG	1 cut(s)	93
AoxI	GGCC	3 cut(s)	385, 515, 1350
ApeKI	GCWGC	5 cut(s)	221, 239, 1000, 1096, 1112
ApoI	RAATTY	4 cut(s)	11, 501, 812, 906
ArsI	GACNNNNNNTTYG	4 cut(s)	338, 370, 690, 722
Asp700I	GAANNNNTTC	3 cut(s)	908, 1154, 1238
AspLEI	GCGC	2 cut(s)	226, 429
AspS9I	GGNCC	3 cut(s)	232, 259, 1351
AsuHPI	GGTGA	4 cut(s)	296, 356, 434, 1138
AvaI	CYCGRG	1 cut(s)	93
AvaII	GGWCC	2 cut(s)	232, 259
BbsI	GAAGAC	2 cut(s)	95, 1293
BbvI	GCAGC	5 cut(s)	233, 251, 1012, 1108, 1124
BccI	CCATC	2 cut(s)	487, 829
BceAI	ACGGC	1 cut(s)	372
BciT130I	CCWGG	3 cut(s)	399, 1124, 1355
BcoDI	GTCTC	3 cut(s)	77, 123, 983
BfaI	CTAG	3 cut(s)	446, 704, 875
BfmI	CTRYAG	1 cut(s)	237
BfoI	RGCGCY	2 cut(s)	227, 430
BisI	GCNGC	6 cut(s)	222, 227, 240, 1001, 1097, 1113
BlpI	GCTNAGC	1 cut(s)	1224
BlsI	GCNGC	6 cut(s)	223, 228, 241, 1002, 1098, 1114
Bme1390I	CCNGG	3 cut(s)	399, 1124, 1355
Bme18I	GGWCC	2 cut(s)	232, 259
BmeT110I	CYCGRG	1 cut(s)	93
BmgT120I	GGNCC	3 cut(s)	232, 259, 1351
BmiI	GGNNCC	3 cut(s)	234, 1307, 1359
BmrFI	CCNGG	3 cut(s)	399, 1124, 1355
BmsI	GCATC	4 cut(s)	241, 1057, 1194, 1210
BpiI	GAAGAC	2 cut(s)	95, 1293
BplI	GAGNNNNNCTC	4 cut(s)	74, 106, 833, 865
BpmI	CTGGAG	3 cut(s)	402, 1376, 1397
Bpu1102I	GCTNAGC	1 cut(s)	1224
BpuEI	CTTGAG	3 cut(s)	662, 908, 1196
BsaHI	GRCGYC	1 cut(s)	1014
BsaI	GGTCTC	1 cut(s)	77
BsaJI	CCNNGG	2 cut(s)	1326, 1353
BsaWI	WCCGGW	1 cut(s)	652
Bsc4I	CCNNNNNNNGG	5 cut(s)	58, 93, 94, 210, 1332
Bse1I	ACTGG	2 cut(s)	385, 1376
BseBI	CCWGG	3 cut(s)	399, 1124, 1355
BseDI	CCNNGG	2 cut(s)	1326, 1353
BseGI	GGATG	7 cut(s)	25, 256, 821, 1060, 1072, 1225, 1279
BseLI	CCNNNNNNNGG	5 cut(s)	58, 93, 94, 210, 1332
BseMII	CTCAG	3 cut(s)	444, 644, 843
BseNI	ACTGG	2 cut(s)	385, 1376
BseRI	GAGGAG	1 cut(s)	1394
BseXI	GCAGC	5 cut(s)	233, 251, 1012, 1108, 1124
BsgI	GTGCAG	2 cut(s)	1094, 1144
Bsh1236I	CGCG	2 cut(s)	193, 465
BshFI	GGCC	3 cut(s)	387, 517, 1352
BsiHKCI	CYCGRG	1 cut(s)	93
BsiSI	CCGG	1 cut(s)	653
BslI	CCNNNNNNNGG	5 cut(s)	58, 93, 94, 210, 1332
BsmAI	GTCTC	3 cut(s)	77, 123, 983
BsmI	GAATGC	1 cut(s)	623
BsnI	GGCC	3 cut(s)	387, 517, 1352
Bso31I	GGTCTC	1 cut(s)	77
BsoBI	CYCGRG	1 cut(s)	93
Bsp143I	GATC	3 cut(s)	286, 768, 1256
Bsp1720I	GCTNAGC	1 cut(s)	1224
BspACI	CCGC	8 cut(s)	193, 205, 227, 248, 511, 784, 1042, 1309
BspANI	GGCC	3 cut(s)	387, 517, 1352
BspCNI	CTCAG	3 cut(s)	445, 643, 844
BspFNI	CGCG	2 cut(s)	193, 465
BspLI	GGNNCC	3 cut(s)	234, 1307, 1359
BspMAI	CTGCAG	1 cut(s)	241
BspPI	GGATC	1 cut(s)	1251
BspQI	GCTCTTC	1 cut(s)	1122
BspTNI	GGTCTC	1 cut(s)	77
BsrI	ACTGG	2 cut(s)	385, 1376
BssECI	CCNNGG	2 cut(s)	1326, 1353
BssMI	GATC	3 cut(s)	286, 768, 1256
BssNI	GRCGYC	1 cut(s)	1014
BssT1I	CCWWGG	1 cut(s)	1326
Bst2UI	CCWGG	3 cut(s)	399, 1124, 1355
Bst4CI	ACNGT	4 cut(s)	344, 661, 994, 1146
Bst6I	CTCTTC	2 cut(s)	1122, 1383
BstACI	GRCGYC	1 cut(s)	1014
BstAPI	GCANNNNNTGC	1 cut(s)	245
BstC8I	GCNNGC	5 cut(s)	191, 364, 603, 1281, 1334
BstDEI	CTNAG	6 cut(s)	453, 630, 852, 864, 883, 1224
BstF5I	GGATG	7 cut(s)	25, 256, 821, 1060, 1072, 1225, 1279
BstFNI	CGCG	2 cut(s)	193, 465
BstH2I	RGCGCY	2 cut(s)	227, 430
BstHHI	GCGC	2 cut(s)	226, 429
BstKTI	GATC	3 cut(s)	289, 771, 1259
BstMAI	GTCTC	3 cut(s)	77, 123, 983
BstMBI	GATC	3 cut(s)	286, 768, 1256
BstMWI	GCNNNNNNNGC	3 cut(s)	245, 1112, 1234
BstNI	CCWGG	3 cut(s)	399, 1124, 1355
BstSCI	CCNGG	3 cut(s)	397, 1122, 1353
BstSFI	CTRYAG	1 cut(s)	237
BstUI	CGCG	2 cut(s)	193, 465
BstV1I	GCAGC	5 cut(s)	233, 251, 1012, 1108, 1124
BstV2I	GAAGAC	2 cut(s)	95, 1293
BstX2I	RGATCY	1 cut(s)	1256
BstYI	RGATCY	1 cut(s)	1256
BsuRI	GGCC	3 cut(s)	387, 517, 1352
BtsCI	GGATG	7 cut(s)	25, 256, 821, 1060, 1072, 1225, 1279
BtsI	GCAGTG	3 cut(s)	261, 1082, 1214
BtsIMutI	CAGTG	4 cut(s)	261, 378, 1082, 1214
Cac8I	GCNNGC	5 cut(s)	191, 364, 603, 1281, 1334
CaiI	CAGNNNCTG	1 cut(s)	637
CfoI	GCGC	2 cut(s)	226, 429
Cfr13I	GGNCC	3 cut(s)	232, 259, 1351
CseI	GACGC	1 cut(s)	1022
Csp6I	GTAC	2 cut(s)	390, 443
CviAII	CATG	2 cut(s)	159, 1134
CviQI	GTAC	2 cut(s)	390, 443
DdeI	CTNAG	6 cut(s)	453, 630, 852, 864, 883, 1224
DpnI	GATC	3 cut(s)	288, 770, 1258
DpnII	GATC	3 cut(s)	286, 768, 1256
Eam1104I	CTCTTC	2 cut(s)	1122, 1383
EarI	CTCTTC	2 cut(s)	1122, 1383
EciI	GGCGGA	1 cut(s)	526
Eco130I	CCWWGG	1 cut(s)	1326
Eco31I	GGTCTC	1 cut(s)	77
Eco47I	GGWCC	2 cut(s)	232, 259
Eco57I	CTGAAG	4 cut(s)	303, 545, 800, 999
Eco88I	CYCGRG	1 cut(s)	93
EcoO109I	RGGNCCY	1 cut(s)	1351
EcoRI	GAATTC	1 cut(s)	812
EcoRII	CCWGG	3 cut(s)	397, 1122, 1353
EcoT14I	CCWWGG	1 cut(s)	1326
ErhI	CCWWGG	1 cut(s)	1326
FaeI	CATG	2 cut(s)	162, 1137
FatI	CATG	2 cut(s)	158, 1133
FauI	CCCGC	2 cut(s)	212, 777
Fnu4HI	GCNGC	6 cut(s)	222, 227, 240, 1001, 1097, 1113
FokI	GGATG	7 cut(s)	32, 263, 808, 1067, 1079, 1232, 1286
Fsp4HI	GCNGC	6 cut(s)	222, 227, 240, 1001, 1097, 1113
FspBI	CTAG	3 cut(s)	446, 704, 875
GlaI	GCGC	2 cut(s)	225, 428
GluI	GCNGC	6 cut(s)	222, 227, 240, 1001, 1097, 1113
GsuI	CTGGAG	3 cut(s)	402, 1376, 1397
HaeII	RGCGCY	2 cut(s)	227, 430
HaeIII	GGCC	3 cut(s)	387, 517, 1352
HapII	CCGG	1 cut(s)	653
HgaI	GACGC	1 cut(s)	1022
HhaI	GCGC	2 cut(s)	226, 429
Hin1I	GRCGYC	1 cut(s)	1014
Hin1II	CATG	2 cut(s)	162, 1137
Hin6I	GCGC	2 cut(s)	224, 427
HinP1I	GCGC	2 cut(s)	224, 427
HindIII	AAGCTT	3 cut(s)	44, 364, 1172
HinfI	GANTC	5 cut(s)	179, 314, 449, 532, 555
HpaII	CCGG	1 cut(s)	653
HphI	GGTGA	4 cut(s)	296, 356, 434, 1138
Hpy166II	GTNNAC	2 cut(s)	392, 1369
Hpy188III	TCNNGA	7 cut(s)	262, 272, 290, 336, 536, 642, 1366
Hpy8I	GTNNAC	2 cut(s)	392, 1369
Hpy99I	CGWCG	1 cut(s)	555
HpyAV	CCTTC	5 cut(s)	71, 569, 863, 1165, 1342
HpyCH4III	ACNGT	4 cut(s)	344, 661, 994, 1146
HpyCH4IV	ACGT	1 cut(s)	171
HpyF10VI	GCNNNNNNNGC	3 cut(s)	245, 1112, 1234
HpyF3I	CTNAG	6 cut(s)	453, 630, 852, 864, 883, 1224
HpySE526I	ACGT	1 cut(s)	171
Hsp92I	GRCGYC	1 cut(s)	1014
Hsp92II	CATG	2 cut(s)	162, 1137
HspAI	GCGC	2 cut(s)	224, 427
Kzo9I	GATC	3 cut(s)	286, 768, 1256
LguI	GCTCTTC	1 cut(s)	1122
LmnI	GCTCC	2 cut(s)	571, 1357
Lsp1109I	GCAGC	5 cut(s)	233, 251, 1012, 1108, 1124
LweI	GCATC	4 cut(s)	241, 1057, 1194, 1210
MaeI	CTAG	3 cut(s)	446, 704, 875
MaeII	ACGT	1 cut(s)	171
MaeIII	GTNAC	1 cut(s)	344
MalI	GATC	3 cut(s)	288, 770, 1258
MboI	GATC	3 cut(s)	286, 768, 1256
MboII	GAAGA	9 cut(s)	95, 309, 607, 721, 808, 913, 1109, 1298, 1400
MfeI	CAATTG	1 cut(s)	36
MflI	RGATCY	1 cut(s)	1256
MluCI	AATT	8 cut(s)	11, 25, 36, 501, 812, 906, 1019, 1248
MlyI	GAGTC	2 cut(s)	188, 458
MroXI	GAANNNNTTC	3 cut(s)	908, 1154, 1238
MseI	TTAA	6 cut(s)	24, 198, 327, 528, 944, 1008
MspA1I	CMGCKG	1 cut(s)	229
MspI	CCGG	1 cut(s)	653
MspR9I	CCNGG	3 cut(s)	399, 1124, 1355
MunI	CAATTG	1 cut(s)	36
Mva1269I	GAATGC	1 cut(s)	623
MvaI	CCWGG	3 cut(s)	399, 1124, 1355
MvnI	CGCG	2 cut(s)	193, 465
MwoI	GCNNNNNNNGC	3 cut(s)	245, 1112, 1234
NdeII	GATC	3 cut(s)	286, 768, 1256
NlaIII	CATG	2 cut(s)	162, 1137
NlaIV	GGNNCC	3 cut(s)	234, 1307, 1359
NmuCI	GTSAC	1 cut(s)	344
PciSI	GCTCTTC	1 cut(s)	1122
PctI	GAATGC	1 cut(s)	623
PdmI	GAANNNNTTC	3 cut(s)	908, 1154, 1238
PfeI	GAWTC	3 cut(s)	314, 532, 555
PkrI	GCNGC	6 cut(s)	223, 228, 241, 1002, 1098, 1114
PleI	GAGTC	2 cut(s)	187, 457
PpsI	GAGTC	2 cut(s)	187, 457
Psp6I	CCWGG	3 cut(s)	397, 1122, 1353
PspGI	CCWGG	3 cut(s)	397, 1122, 1353
PspN4I	GGNNCC	3 cut(s)	234, 1307, 1359
PspPI	GGNCC	3 cut(s)	232, 259, 1351
PstI	CTGCAG	1 cut(s)	241
PstNI	CAGNNNCTG	1 cut(s)	637
PsuI	RGATCY	1 cut(s)	1256
RsaI	GTAC	2 cut(s)	391, 444
RsaNI	GTAC	2 cut(s)	390, 443
SapI	GCTCTTC	1 cut(s)	1122
SaqAI	TTAA	6 cut(s)	24, 198, 327, 528, 944, 1008
SatI	GCNGC	6 cut(s)	222, 227, 240, 1001, 1097, 1113
Sau3AI	GATC	3 cut(s)	286, 768, 1256
Sau96I	GGNCC	3 cut(s)	232, 259, 1351
SchI	GAGTC	2 cut(s)	188, 458
ScrFI	CCNGG	3 cut(s)	399, 1124, 1355
SfaNI	GCATC	4 cut(s)	241, 1057, 1194, 1210
SfcI	CTRYAG	1 cut(s)	237
SinI	GGWCC	2 cut(s)	232, 259
SmlI	CTYRAG	3 cut(s)	677, 923, 1175
SmoI	CTYRAG	3 cut(s)	677, 923, 1175
Sse9I	AATT	8 cut(s)	11, 25, 36, 501, 812, 906, 1019, 1248
SsiI	CCGC	8 cut(s)	193, 205, 227, 248, 511, 784, 1042, 1309
SspI	AATATT	1 cut(s)	217
SspMI	CTAG	3 cut(s)	446, 704, 875
StyD4I	CCNGG	3 cut(s)	397, 1122, 1353
StyI	CCWWGG	1 cut(s)	1326
TaaI	ACNGT	4 cut(s)	344, 661, 994, 1146
TaiI	ACGT	1 cut(s)	174
TaqI	TCGA	3 cut(s)	75, 537, 912
TasI	AATT	8 cut(s)	11, 25, 36, 501, 812, 906, 1019, 1248
TatI	WGTACW	1 cut(s)	442
TauI	GCSGC	1 cut(s)	229
TfiI	GAWTC	3 cut(s)	314, 532, 555
Tru1I	TTAA	6 cut(s)	24, 198, 327, 528, 944, 1008
Tru9I	TTAA	6 cut(s)	24, 198, 327, 528, 944, 1008
TscAI	CASTG	4 cut(s)	261, 385, 1082, 1214
TseFI	GTSAC	1 cut(s)	344
TseI	GCWGC	5 cut(s)	221, 239, 1000, 1096, 1112
Tsp45I	GTSAC	1 cut(s)	344
TspDTI	ATGAA	6 cut(s)	147, 603, 706, 947, 1247, 1281
TspGWI	ACGGA	1 cut(s)	226
TspRI	CASTG	4 cut(s)	261, 385, 1082, 1214
VpaK11BI	GGWCC	2 cut(s)	232, 259
XapI	RAATTY	4 cut(s)	11, 501, 812, 906
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1368
XmnI	GAANNNNTTC	3 cut(s)	908, 1154, 1238
XspI	CTAG	3 cut(s)	446, 704, 875

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.