pycom04g21600

Basic leucine zipper and W2 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0006554
Physical Location & Seq
Forward (+)
22683756 .. 22686236
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21600.1

Sequence Viewer

Length: 765 bp
ATGAGGTACCTTCTCTCTCTCTCTCTCTCTCTCTATCGATCTTTCCGTTCCACTTCTACAGTCTCGAAGGAGAGACCCACTCTCGGTGGCACGCGGATTAAGACCCGCAAACGGAATATTGCAGCACCGCTGGACACTGCAGCTTTTGCGGATGCAGTGGTCCAGATTTATCTGGATAATGCTGGTGATCTGGAACTTATTGCCAAGAGCATTGAATCTTCAGACCTTAACTTTTCAAGATACGGTGACACCTTTTTTGAGGTTGTTTTCACTGGAGGCCGTACACAACCTGGAACAACAAAACCTGATGAGGGGGAGCGCCACCCTTATTCTGTACTAGAGTCTGAGCCTACACGTGAACTCATCTTGCCATCTGTTATCTACATTCAAAAAATTTTGAGGCGGAGGCCATTTCTGATTAAGAATCTCGAAAATGTTATGCGACGATTCCTTCAATCATTGGAGCTTTTTGAGGAAAATGAAAGGAAGAAGCTGGCTATTTTCACAGCACTTGCATTCTCTCAGAAGCTGTCAGGACTTCCACCGGAAACGGTGTTCCAGCCAATGCTCAAGGATAATCTTGTTGGCAAAGGGCTAGTCCTATTGTTCATTACTGACTTCTTCAAGGAGTATTTGATTGATAATAGCCTTGATGATTTGATCTCCATTCTGAAGCGGGGTAAAGTGGAAGACAATCTTTTGGAATTCTTCCCATCCACAAAAAGAACTGATGAGAGTTTCTCTGAGCATTTCACGAAGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
W2

Protein Analysis

255

Amino Acids

29.03

Weight (kDa)

8.65

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_5MP1_2 PF25504 33 - 253 3.9e-75 5MP1/2 HEAT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 6
AccB1I GGYRCC 1 cut(s) 6
AccII CGCG 1 cut(s) 94
AciI CCGC 6 cut(s) 94, 106, 128, 149, 403, 676
AcsI RAATTY 2 cut(s) 393, 704
AcuI CTGAAG 2 cut(s) 204, 692
AcvI CACGTG 1 cut(s) 356
AfaI GTAC 3 cut(s) 8, 283, 336
AfiI CCNNNNNNNGG 3 cut(s) 83, 111, 311
AflIII ACRYGT 1 cut(s) 353
AgsI TTSAA 5 cut(s) 215, 237, 389, 455, 625
AjnI CCWGG 1 cut(s) 289
AloI GAACNNNNNNTCC 2 cut(s) 539, 571
AluBI AGCT 4 cut(s) 143, 466, 493, 529
AluI AGCT 4 cut(s) 143, 466, 493, 529
Alw26I GTCTC 2 cut(s) 67, 67
AlwNI CAGNNNCTG 1 cut(s) 529
AoxI GGCC 2 cut(s) 277, 407
ApeKI GCWGC 2 cut(s) 122, 140
ApoI RAATTY 2 cut(s) 393, 704
ArsI GACNNNNNNTTYG 4 cut(s) 239, 271, 582, 614
Asp718I GGTACC 1 cut(s) 6
AspLEI GCGC 1 cut(s) 321
AspS9I GGNCC 1 cut(s) 160
AsuHPI GGTGA 2 cut(s) 197, 257
AvaII GGWCC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 6
BbrPI CACGTG 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 696
BbvI GCAGC 2 cut(s) 134, 152
BccI CCATC 2 cut(s) 379, 721
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 67, 67
BfaI CTAG 3 cut(s) 338, 596, 763
BfmI CTRYAG 2 cut(s) 57, 138
BfoI RGCGCY 1 cut(s) 322
BisI GCNGC 2 cut(s) 123, 141
BlsI GCNGC 2 cut(s) 124, 142
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 8
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 696
BplI GAGNNNNNCTC 4 cut(s) 64, 96, 725, 757
BpmI CTGGAG 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 554
Bsa29I ATCGAT 1 cut(s) 37
BsaAI YACGTR 1 cut(s) 356
BsaI GGTCTC 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 544
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 111, 311
Bse1I ACTGG 1 cut(s) 277
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 1 cut(s) 37
BseGI GGATG 2 cut(s) 157, 713
BseLI CCNNNNNNNGG 3 cut(s) 83, 111, 311
BseMII CTCAG 3 cut(s) 336, 536, 735
BseNI ACTGG 1 cut(s) 277
BseXI GCAGC 2 cut(s) 134, 152
Bsh1236I CGCG 1 cut(s) 94
BshFI GGCC 2 cut(s) 279, 409
BshNI GGYRCC 1 cut(s) 6
BshVI ATCGAT 1 cut(s) 37
BsiSI CCGG 1 cut(s) 545
BslI CCNNNNNNNGG 3 cut(s) 83, 111, 311
BsmAI GTCTC 2 cut(s) 67, 67
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 2 cut(s) 279, 409
Bso31I GGTCTC 1 cut(s) 67
Bsp143I GATC 3 cut(s) 38, 187, 660
BspACI CCGC 6 cut(s) 94, 106, 128, 149, 403, 676
BspANI GGCC 2 cut(s) 279, 409
BspCNI CTCAG 3 cut(s) 337, 535, 736
BspDI ATCGAT 1 cut(s) 37
BspFNI CGCG 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 8
BspMAI CTGCAG 1 cut(s) 142
BspT107I GGYRCC 1 cut(s) 6
BspTNI GGTCTC 1 cut(s) 67
BsrI ACTGG 1 cut(s) 277
BssMI GATC 3 cut(s) 38, 187, 660
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 61, 245, 553
BstAPI GCANNNNNTGC 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 92, 495
BstDEI CTNAG 3 cut(s) 345, 522, 744
BstF5I GGATG 2 cut(s) 157, 713
BstFNI CGCG 1 cut(s) 94
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 3 cut(s) 41, 190, 663
BstMAI GTCTC 2 cut(s) 67, 67
BstMBI GATC 3 cut(s) 38, 187, 660
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 2 cut(s) 57, 138
BstUI CGCG 1 cut(s) 94
BstV1I GCAGC 2 cut(s) 134, 152
BstV2I GAAGAC 1 cut(s) 696
Bsu15I ATCGAT 1 cut(s) 37
BsuRI GGCC 2 cut(s) 279, 409
BsuTUI ATCGAT 1 cut(s) 37
BtsCI GGATG 2 cut(s) 157, 713
BtsI GCAGTG 2 cut(s) 135, 162
BtsIMutI CAGTG 3 cut(s) 135, 162, 270
Cac8I GCNNGC 2 cut(s) 92, 495
CaiI CAGNNNCTG 1 cut(s) 529
CfoI GCGC 1 cut(s) 321
Cfr13I GGNCC 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 37
Csp6I GTAC 3 cut(s) 7, 282, 335
CviQI GTAC 3 cut(s) 7, 282, 335
DdeI CTNAG 3 cut(s) 345, 522, 744
DpnI GATC 3 cut(s) 40, 189, 662
DpnII GATC 3 cut(s) 38, 187, 660
EciI GGCGGA 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 204, 692
Eco72I CACGTG 1 cut(s) 356
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 1 cut(s) 289
FaiI YATR 1 cut(s) 440
FalI AAGNNNNNCTT 2 cut(s) 681, 713
FauI CCCGC 2 cut(s) 113, 669
Fnu4HI GCNGC 2 cut(s) 123, 141
FokI GGATG 2 cut(s) 164, 700
Fsp4HI GCNGC 2 cut(s) 123, 141
FspBI CTAG 3 cut(s) 338, 596, 763
GlaI GCGC 1 cut(s) 320
GluI GCNGC 2 cut(s) 123, 141
GsuI CTGGAG 1 cut(s) 294
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 2 cut(s) 279, 409
HapII CCGG 1 cut(s) 545
HhaI GCGC 1 cut(s) 321
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HinfI GANTC 4 cut(s) 215, 341, 424, 447
HpaII CCGG 1 cut(s) 545
HphI GGTGA 2 cut(s) 197, 257
Hpy166II GTNNAC 2 cut(s) 284, 359
Hpy188I TCNGA 6 cut(s) 223, 346, 417, 525, 672, 745
Hpy188III TCNNGA 8 cut(s) 64, 163, 173, 191, 237, 428, 534, 754
Hpy8I GTNNAC 2 cut(s) 284, 359
Hpy99I CGWCG 1 cut(s) 447
HpyAV CCTTC 4 cut(s) 20, 61, 461, 751
HpyCH4III ACNGT 3 cut(s) 61, 245, 553
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 4 cut(s) 122, 140, 155, 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 3 cut(s) 345, 522, 744
HpySE526I ACGT 1 cut(s) 355
HspAI GCGC 1 cut(s) 319
KpnI GGTACC 1 cut(s) 10
Kzo9I GATC 3 cut(s) 38, 187, 660
LmnI GCTCC 2 cut(s) 316, 463
Lsp1109I GCAGC 2 cut(s) 134, 152
LweI GCATC 1 cut(s) 142
MaeI CTAG 3 cut(s) 338, 596, 763
MaeII ACGT 1 cut(s) 355
MaeIII GTNAC 1 cut(s) 245
MalI GATC 3 cut(s) 40, 189, 662
MboI GATC 3 cut(s) 38, 187, 660
MboII GAAGA 5 cut(s) 210, 499, 613, 700, 701
MluCI AATT 2 cut(s) 393, 704
MlyI GAGTC 1 cut(s) 350
MnlI CCTC 6 cut(s) 253, 269, 304, 393, 399, 466
MseI TTAA 3 cut(s) 99, 228, 420
MspA1I CMGCKG 1 cut(s) 130
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 1 cut(s) 291
Mva1269I GAATGC 1 cut(s) 515
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 1 cut(s) 94
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeII GATC 3 cut(s) 38, 187, 660
NlaIV GGNNCC 1 cut(s) 8
NmuCI GTSAC 1 cut(s) 245
PcsI WCGNNNNNNNCGW 1 cut(s) 43
PctI GAATGC 1 cut(s) 515
PfeI GAWTC 3 cut(s) 215, 424, 447
PkrI GCNGC 2 cut(s) 124, 142
PleI GAGTC 1 cut(s) 349
PmaCI CACGTG 1 cut(s) 356
PmlI CACGTG 1 cut(s) 356
PpsI GAGTC 1 cut(s) 349
Ppu21I YACGTR 1 cut(s) 356
Psp6I CCWGG 1 cut(s) 289
PspCI CACGTG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 8
PspPI GGNCC 1 cut(s) 160
PstI CTGCAG 1 cut(s) 142
PstNI CAGNNNCTG 1 cut(s) 529
RsaI GTAC 3 cut(s) 8, 283, 336
RsaNI GTAC 3 cut(s) 7, 282, 335
SaqAI TTAA 3 cut(s) 99, 228, 420
SatI GCNGC 2 cut(s) 123, 141
Sau3AI GATC 3 cut(s) 38, 187, 660
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 1 cut(s) 291
SfaNI GCATC 1 cut(s) 142
SfcI CTRYAG 2 cut(s) 57, 138
SinI GGWCC 1 cut(s) 160
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 2 cut(s) 393, 704
SsiI CCGC 6 cut(s) 94, 106, 128, 149, 403, 676
SspI AATATT 1 cut(s) 118
SspMI CTAG 3 cut(s) 338, 596, 763
StyD4I CCNGG 1 cut(s) 289
TaaI ACNGT 3 cut(s) 61, 245, 553
TaiI ACGT 1 cut(s) 358
TaqI TCGA 3 cut(s) 37, 65, 429
TasI AATT 2 cut(s) 393, 704
TatI WGTACW 1 cut(s) 334
TfiI GAWTC 3 cut(s) 215, 424, 447
Tru1I TTAA 3 cut(s) 99, 228, 420
Tru9I TTAA 3 cut(s) 99, 228, 420
TscAI CASTG 3 cut(s) 142, 162, 277
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 2 cut(s) 122, 140
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 2 cut(s) 495, 598
TspGWI ACGGA 2 cut(s) 35, 127
TspRI CASTG 3 cut(s) 142, 162, 277
VpaK11BI GGWCC 1 cut(s) 160
XapI RAATTY 2 cut(s) 393, 704
XspI CTAG 3 cut(s) 338, 596, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.