MD01G1068300.v1.1

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006847
Physical Location & Seq
Reverse (-)
17223903 .. 17229539
5637 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1068300.v1.1.491

Sequence Viewer

Length: 2940 bp
ATGAAAATCAATCTGAAAGATCCAGCTGAAAGCCTCTACCCATGGACCTCCACCAAGATCACAATATGTTTCTTGCTTGTGATCTCCTTCACCTATATCTCTTACTCTCTAAAATCTGTCTTACCTTCCAATCTTTGTAGGGAACAATATTCTGTCACCTCCCATCTCCAATCCGAGGCTCCCGCCACCCCATCTCAGCCGATATCCCCACGGGCACAGGCAACCCTGGAACCCCAAGAGCCCAAAACTAACTTATCCCACATAGTGTTTGGCATTGCAGCCTCGGCCAATCTGTGGGGAAGTAGAAAAAAATACGTGAAGCTATGGTGGAGGCCGGACCAAATGAGAGGCAATGTTTGGATGGATCAGCCAGTAAAAGTTGAACCGGAGGATGAACACCTTTTGCCGCCAATAAAGATATCTAGTGACACTTCCAAGTTTGCATACACTCACCGTAAAGGCCACAAGTCAGCCATACGCTTGTCACGTATTGTTACGGAGAATTTCAGGCAAGGCATGGAGAATGTTAGGTGGTTTGTGATGGGGGATGATGACACAGTTTTTGTTCCTGAAAATCTGGTTAAGGTTTTGCAGAAATATGACCATAACCAATTTTATTACATCGGAAGCTCGTCGGAGAGTCACTTGCAGAACATTTATTTTTCACATAATATGGCTTATGGAGGAGGTGGGATTGCTATAAGTTACCCTCTAGCCAGAGCCCTAGAGAAAATGCAAGACAAGTGCATTGCTCGGTATACTGGATTGTACGGCTCGGATGATAGGCTTCAGGCTTGTATGGCTGAGCTTGGTGTTCCTCTAACCAAAGAACAAGGGTTTCACCAGTTTGATGTATATGGGAGCGTGTTCGGACTCCTAGCAGCTCATCCGGTCGCGCCCCTTGTGTCACTTCACCACCTAGATGTCGTTGATCCTATATTCCCCAACATGGACCGAGTCCAAGCCCTACAAAGGCTTAACGTTCCCATGAAGATCGACCCGGCCGGACTCGTTCAACAATCCATTTGCTACGACAAGACCCGATCCTGGACTGTATCTGTTTCATGGGGCTATGCGGTTCAAATATTCCGGGGCATTTTCTCTCCCCGAGAAATAGAACAGCCTGCCAGAACGTTCATCAATTGGTACAAGAAAGCTGACTTCATTGGCTACGAATTCAACACTCGTCCCGTCAGTAGACACCCTTGTCAAAAACCATTTGTGTATTACTTGACGAATGCTGTGTACAACAAAAATACAAATCATACAGCAACTGAATATGTTCGTGATCAAGCGCCAAATCCTCAATGCAACTGGAAGATGGCAAATCCCTCTCGCATTGACAGAGTACAAGTCTACAAAGCATATGATCCGCATATATGGGACAAGTCTCCAAGGAGAAACTGTTGCAGAATCTTGCCAAGTAAAAAGAAGCAAGGTACCATGGCCATTGAGGTAGGAGCATGCAATGAAGATGAAGCGGTTCTCAAAATGAGGACCTTAAGAGGGTTGCAGACTTCCCCTCTCATGGAGTTTCCGTACAGAAAGCTCCTCTTCTCTCTCTCCCTACTTTCTCTCTCTACCATTTCCGCCCTCTCTCGCTCCTCCTTCTCCCCAATAGACAACTACCTCGTCGACTGCGGCTCCACCGCGGAATCCACCGTCGACAACCGCCGCTTCGTTCCCGACTCGTCCAAGCCCGACTCCTCCCTGTCCTCCTCAACTCGCTCCATCTCTCTCAGAAATGAAAACCCAGTTCCCAATTCGCCCCAAATCTACAGCACCGCCAGGGCTTTCAGGCGGCCGTCCAAGTACGAGTTCCAGATCCGCGACGCGGGGACCCACATGGTACGCCTCCATTTCCAGACCATTAATTCTTTGAAATTGGATTGGAATGATGCTCAATTTCATGTTTTGGTCAATGGGTTTGTGGCTTTGACCGACTTTAGAGCTGCAAAAATCCCCATGGTTATGGAGTTTTTGATCTGGGTCGATACCAAAAAGCTTGTAATTGAATTTGTTCCTGCAAAAAAATCGAACTTTGCGTTTGTGAATGCGATTGAGGTGATTTCTGCGCCTAAGGATCTTGTTGCTGATACAGCAAAGTTTGTGAGTGATGAGAAAGCTGAGAATTTTAACGGGTTAATTAAGCAAGCGCTTCAGGTTGTGTATAGGGTTAATGTGGGAGGTTCGAAAGTGACGCCGTTTAATGATTCTCTGTGGAGGACTTGGGTTACTGATGATGAGTATTTGGAATCGGATTCCGGGTCGGAAAGGGTGTATTTTGGTGGTCGGATTAATTATCAGGATGGAGGGGCTAGCAGGGAAGTTGGTCCTGATAATGTGTATAATACTGCCCGGCTGATTCATAGTTCGAATGCTTCGATTCCGAATGTCAATATGACATGGGTTTTTCCTGCTGTTGGAGGATATAAATATCTTGTTCGGTTGCATTTTTGTGACATTGCTAGCATTTCGATCGGATTGCTGTATTTCAATGTTTATGTGAATGGGAAATTGGCATATGAGAATTTGGATTTGTCTATGGCAACGAATTATATGCTGGCTTCTCCGTTCTATGCGGATTTCGTGGTTGATGGAGAGGAATCGGGTGTATTGTCCGTAAGTGCAGGACCTTCGAATATGAGTATGCCTTATGCAATTGATGGGATTTTGAATGGGATTGAGATCATGAGGTTGAATAACTCCGTGGGGAGTCTTGACGGCAAGCACTGTGCCGGGTGGGTTCTGAGGAACTGGCCGAAAGGAAATGTTGGTGCGGCGGTTCCTTTGGTTGCTGCCGCCTGTTTGCTGCTGAGTATTTCATTGGTCATGCGTAGGAGGATGAGCGGGAAGGAATTCGTGGGATGGTCGAAACTGCCCACAAGTGTTTCAGAAGTTAATCTCAAGCATGGAAACACACAGCTTTCGGTTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604 Malectin_like

Protein Analysis

980

Amino Acids

110.22

Weight (kDa)

8.97

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 164 - 282 2.4e-15 Fringe-like
DUF604 PF04646 217 - 470 3.6e-116 Protein of unknown function, DUF604
Malectin PF11721 544 - 649 1.7e-06 Malectin domain
Malectin_like PF12819 545 - 899 1.6e-26 Malectin-like domain
Malectin PF11721 722 - 851 4.5e-07 Malectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1206
Acc65I GGTACC 1 cut(s) 1439
AccB1I GGYRCC 1 cut(s) 1439
AccB7I CCANNNNNTGG 1 cut(s) 294
AccBSI CCGCTC 1 cut(s) 2850
AccI GTMKAC 5 cut(s) 758, 1198, 1356, 1635, 1665
AccII CGCG 4 cut(s) 896, 1652, 1830, 1835
AclI AACGTT 2 cut(s) 981, 1133
AclWI GGATC 7 cut(s) 14, 372, 926, 1038, 1364, 1819, 2091
AcoI YGGCCR 5 cut(s) 285, 1002, 1446, 1802, 2759
AcsI RAATTY 6 cut(s) 502, 1175, 2015, 2131, 2530, 2858
AcuI CTGAAG 2 cut(s) 773, 2144
AcyI GRCGYC 1 cut(s) 2201
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 8 cut(s) 770, 1148, 1247, 1350, 1441, 1541, 1814, 1851
AfeI AGCGCT 1 cut(s) 2157
AfiI CCNNNNNNNGG 9 cut(s) 175, 294, 949, 972, 1047, 1506, 1528, 1834, 2423
AflII CTTAAG 1 cut(s) 1501
AgsI TTSAA 9 cut(s) 383, 1016, 1082, 1180, 1882, 2015, 2497, 2677, 2701
AjnI CCWGG 3 cut(s) 225, 1046, 1787
AjuI GAANNNNNNNTTGG 4 cut(s) 1802, 1834, 2501, 2533
Alw26I GTCTC 1 cut(s) 1395
AlwI GGATC 7 cut(s) 14, 372, 926, 1038, 1364, 1819, 2091
AlwNI CAGNNNCTG 1 cut(s) 1274
Ama87I CYCGRG 1 cut(s) 1107
Aor51HI AGCGCT 1 cut(s) 2157
AoxI GGCC 7 cut(s) 285, 332, 460, 1002, 1446, 1802, 2759
ApeKI GCWGC 5 cut(s) 278, 881, 1952, 2798, 2812
ApoI RAATTY 6 cut(s) 502, 1175, 2015, 2131, 2530, 2858
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AseI ATTAAT 2 cut(s) 1872, 2298
Asp700I GAANNNNTTC 4 cut(s) 1281, 1482, 2019, 2858
Asp718I GGTACC 1 cut(s) 1439
AspLEI GCGC 4 cut(s) 898, 1297, 2077, 2158
AspS9I GGNCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
AsuC2I CCSGG 5 cut(s) 1001, 1091, 2266, 2359, 2740
AsuHPI GGTGA 6 cut(s) 82, 148, 443, 833, 905, 2077
AsuII TTCGAA 3 cut(s) 2192, 2375, 2639
AsuNHI GCTAGC 2 cut(s) 2318, 2468
AvaI CYCGRG 1 cut(s) 1107
AvaII GGWCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
AxyI CCTNAGG 1 cut(s) 2079
BaeGI GKGCMC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 1448
BanI GGYRCC 1 cut(s) 1439
BanII GRGCYC 2 cut(s) 243, 724
BbvI GCAGC 5 cut(s) 290, 893, 1939, 2785, 2799
BceAI ACGGC 4 cut(s) 787, 1789, 2188, 2740
BciT130I CCWGG 3 cut(s) 227, 1048, 1789
BclI TGATCA 1 cut(s) 1288
BcnI CCSGG 5 cut(s) 1001, 1091, 2266, 2359, 2740
BcoDI GTCTC 1 cut(s) 1395
BfaI CTAG 7 cut(s) 423, 713, 725, 878, 920, 2319, 2469
BfmI CTRYAG 1 cut(s) 1777
BfoI RGCGCY 2 cut(s) 1298, 2159
BfrI CTTAAG 1 cut(s) 1501
BlpI GCTNAGC 1 cut(s) 804
Bme1390I CCNGG 8 cut(s) 227, 1001, 1048, 1091, 1789, 2266, 2359, 2740
Bme18I GGWCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
BmeT110I CYCGRG 1 cut(s) 1107
BmgT120I GGNCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
BmiI GGNNCC 7 cut(s) 180, 231, 1441, 1645, 1840, 1841, 2787
BmrFI CCNGG 8 cut(s) 227, 1001, 1048, 1091, 1789, 2266, 2359, 2740
BmrI ACTGGG 1 cut(s) 1748
BmsI GCATC 1 cut(s) 1888
BmtI GCTAGC 2 cut(s) 2322, 2472
BmuI ACTGGG 1 cut(s) 1748
Bpu1102I GCTNAGC 1 cut(s) 804
Bpu14I TTCGAA 3 cut(s) 2192, 2375, 2639
BpuEI CTTGAG 1 cut(s) 2891
BpuMI CCSGG 5 cut(s) 1001, 1091, 2266, 2359, 2740
BsaAI YACGTR 2 cut(s) 316, 488
BsaBI GATNNNNATC 1 cut(s) 2687
BsaHI GRCGYC 1 cut(s) 2201
BsaWI WCCGGW 2 cut(s) 385, 889
BsaXI ACNNNNNCTCC 6 cut(s) 1390, 1420, 1523, 1553, 1628, 1658
Bsc4I CCNNNNNNNGG 9 cut(s) 175, 294, 949, 972, 1047, 1506, 1528, 1834, 2423
Bse1I ACTGG 6 cut(s) 371, 766, 844, 1319, 1754, 2762
Bse21I CCTNAGG 1 cut(s) 2079
Bse3DI GCAATG 5 cut(s) 273, 358, 747, 1474, 2463
Bse8I GATNNNNATC 1 cut(s) 2687
BseBI CCWGG 3 cut(s) 227, 1048, 1789
BseGI GGATG 8 cut(s) 366, 397, 553, 784, 886, 2314, 2850, 2873
BseJI GATNNNNATC 1 cut(s) 2687
BseLI CCNNNNNNNGG 9 cut(s) 175, 294, 949, 972, 1047, 1506, 1528, 1834, 2423
BseMI GCAATG 5 cut(s) 273, 358, 747, 1474, 2463
BseMII CTCAG 6 cut(s) 209, 795, 1753, 2118, 2741, 2807
BseNI ACTGG 6 cut(s) 371, 766, 844, 1319, 1754, 2762
BseRI GAGGAG 5 cut(s) 699, 1541, 1594, 1696, 1708
BseSI GKGCMC 1 cut(s) 217
BseX3I CGGCCG 2 cut(s) 1002, 1802
BseXI GCAGC 5 cut(s) 290, 893, 1939, 2785, 2799
BsgI GTGCAG 1 cut(s) 2649
Bsh1236I CGCG 4 cut(s) 896, 1652, 1830, 1835
Bsh1285I CGRYCG 4 cut(s) 894, 1005, 1805, 2481
BshFI GGCC 7 cut(s) 287, 334, 462, 1004, 1448, 1804, 2761
BshNI GGYRCC 1 cut(s) 1439
BsiEI CGRYCG 4 cut(s) 894, 1005, 1805, 2481
BsiHKCI CYCGRG 1 cut(s) 1107
BsiSI CCGG 9 cut(s) 335, 386, 890, 1001, 1005, 1090, 2265, 2359, 2739
BslFI GGGAC 3 cut(s) 1173, 1397, 1852
BslI CCNNNNNNNGG 9 cut(s) 175, 294, 949, 972, 1047, 1506, 1528, 1834, 2423
BsmAI GTCTC 1 cut(s) 1395
BsmFI GGGAC 3 cut(s) 1173, 1397, 1852
BsmI GAATGC 3 cut(s) 1243, 2059, 2383
BsnI GGCC 7 cut(s) 287, 334, 462, 1004, 1448, 1804, 2761
BsoBI CYCGRG 1 cut(s) 1107
Bsp119I TTCGAA 3 cut(s) 2192, 2375, 2639
Bsp1286I GDGCHC 3 cut(s) 217, 243, 724
Bsp1407I TGTACA 1 cut(s) 1245
Bsp1720I GCTNAGC 1 cut(s) 804
Bsp19I CCATGG 3 cut(s) 41, 1443, 1965
BspANI GGCC 7 cut(s) 287, 334, 462, 1004, 1448, 1804, 2761
BspCNI CTCAG 6 cut(s) 208, 796, 1752, 2119, 2742, 2808
BspFNI CGCG 4 cut(s) 896, 1652, 1830, 1835
BspHI TCATGA 1 cut(s) 2691
BspLI GGNNCC 7 cut(s) 180, 231, 1441, 1645, 1840, 1841, 2787
BspOI GCTAGC 2 cut(s) 2322, 2472
BspPI GGATC 7 cut(s) 14, 372, 926, 1038, 1364, 1819, 2091
BspT104I TTCGAA 3 cut(s) 2192, 2375, 2639
BspT107I GGYRCC 1 cut(s) 1439
BspTI CTTAAG 1 cut(s) 1501
BsrBI CCGCTC 1 cut(s) 2850
BsrDI GCAATG 5 cut(s) 273, 358, 747, 1474, 2463
BsrGI TGTACA 1 cut(s) 1245
BsrI ACTGG 6 cut(s) 371, 766, 844, 1319, 1754, 2762
BssNAI GTATAC 1 cut(s) 759
BssNI GRCGYC 1 cut(s) 2201
BssT1I CCWWGG 4 cut(s) 41, 1394, 1443, 1965
Bst1107I GTATAC 1 cut(s) 759
Bst2UI CCWGG 3 cut(s) 227, 1048, 1789
Bst4CI ACNGT 6 cut(s) 455, 559, 1054, 1406, 1663, 2735
Bst6I CTCTTC 1 cut(s) 1559
BstACI GRCGYC 1 cut(s) 2201
BstAFI CTTAAG 1 cut(s) 1501
BstAUI TGTACA 1 cut(s) 1245
BstBAI YACGTR 2 cut(s) 316, 488
BstBI TTCGAA 3 cut(s) 2192, 2375, 2639
BstC8I GCNNGC 7 cut(s) 1125, 1465, 2154, 2320, 2470, 2565, 2729
BstDEI CTNAG 7 cut(s) 195, 804, 1739, 2079, 2127, 2750, 2816
BstDSI CCRYGG 6 cut(s) 41, 209, 1443, 1650, 1965, 2709
BstF5I GGATG 8 cut(s) 366, 397, 553, 784, 886, 2314, 2850, 2873
BstFNI CGCG 4 cut(s) 896, 1652, 1830, 1835
BstH2I RGCGCY 2 cut(s) 1298, 2159
BstHHI GCGC 4 cut(s) 898, 1297, 2077, 2158
BstMAI GTCTC 1 cut(s) 1395
BstMCI CGRYCG 4 cut(s) 894, 1005, 1805, 2481
BstMWI GCNNNNNNNGC 3 cut(s) 284, 800, 2099
BstNI CCWGG 3 cut(s) 227, 1048, 1789
BstNSI RCATGY 1 cut(s) 1467
BstSCI CCNGG 8 cut(s) 225, 999, 1046, 1089, 1787, 2264, 2357, 2738
BstSFI CTRYAG 1 cut(s) 1777
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 4 cut(s) 896, 1652, 1830, 1835
BstV1I GCAGC 5 cut(s) 290, 893, 1939, 2785, 2799
BstX2I RGATCY 3 cut(s) 19, 1824, 2083
BstXI CCANNNNNNTGG 1 cut(s) 1972
BstYI RGATCY 3 cut(s) 19, 1824, 2083
BstZ17I GTATAC 1 cut(s) 759
BstZI CGGCCG 2 cut(s) 1002, 1802
Bsu36I CCTNAGG 1 cut(s) 2079
BsuRI GGCC 7 cut(s) 287, 334, 462, 1004, 1448, 1804, 2761
BtgI CCRYGG 6 cut(s) 41, 209, 1443, 1650, 1965, 2709
BtsCI GGATG 8 cut(s) 366, 397, 553, 784, 886, 2314, 2850, 2873
BtsIMutI CAGTG 1 cut(s) 2731
Cac8I GCNNGC 7 cut(s) 1125, 1465, 2154, 2320, 2470, 2565, 2729
CaiI CAGNNNCTG 1 cut(s) 1274
CciI TCATGA 1 cut(s) 2691
CfoI GCGC 4 cut(s) 898, 1297, 2077, 2158
Cfr13I GGNCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
Cfr42I CCGCGG 1 cut(s) 1653
CseI GACGC 2 cut(s) 1841, 2209
Csp6I GTAC 8 cut(s) 769, 1147, 1246, 1349, 1440, 1540, 1813, 1850
CviQI GTAC 8 cut(s) 769, 1147, 1246, 1349, 1440, 1540, 1813, 1850
DdeI CTNAG 7 cut(s) 195, 804, 1739, 2079, 2127, 2750, 2816
DraIII CACNNNGTG 1 cut(s) 265
DrdI GACNNNNNNGTC 1 cut(s) 1206
DseDI GACNNNNNNGTC 1 cut(s) 1206
EaeI YGGCCR 5 cut(s) 285, 1002, 1446, 1802, 2759
EagI CGGCCG 2 cut(s) 1002, 1802
Eam1104I CTCTTC 1 cut(s) 1559
EarI CTCTTC 1 cut(s) 1559
EciI GGCGGA 1 cut(s) 1579
EclXI CGGCCG 2 cut(s) 1002, 1802
Eco130I CCWWGG 4 cut(s) 41, 1394, 1443, 1965
Eco24I GRGCYC 2 cut(s) 243, 724
Eco32I GATATC 2 cut(s) 204, 420
Eco47I GGWCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
Eco47III AGCGCT 1 cut(s) 2157
Eco52I CGGCCG 2 cut(s) 1002, 1802
Eco57I CTGAAG 2 cut(s) 773, 2144
Eco81I CCTNAGG 1 cut(s) 2079
Eco88I CYCGRG 1 cut(s) 1107
EcoO109I RGGNCCY 3 cut(s) 1497, 1839, 2633
EcoRI GAATTC 2 cut(s) 1175, 2858
EcoRII CCWGG 3 cut(s) 225, 1046, 1787
EcoRV GATATC 2 cut(s) 204, 420
EcoT14I CCWWGG 4 cut(s) 41, 1394, 1443, 1965
EcoT38I GRGCYC 2 cut(s) 243, 724
ErhI CCWWGG 4 cut(s) 41, 1394, 1443, 1965
FalI AAGNNNNNCTT 2 cut(s) 1538, 1570
FaqI GGGAC 3 cut(s) 1173, 1397, 1852
FauI CCCGC 3 cut(s) 190, 1828, 2843
FauNDI CATATG 2 cut(s) 1366, 2524
FbaI TGATCA 1 cut(s) 1288
FblI GTMKAC 5 cut(s) 758, 1198, 1356, 1635, 1665
FokI GGATG 8 cut(s) 373, 404, 560, 791, 873, 2321, 2857, 2880
FriOI GRGCYC 2 cut(s) 243, 724
FspBI CTAG 7 cut(s) 423, 713, 725, 878, 920, 2319, 2469
GlaI GCGC 4 cut(s) 897, 1296, 2076, 2157
HaeII RGCGCY 2 cut(s) 1298, 2159
HaeIII GGCC 7 cut(s) 287, 334, 462, 1004, 1448, 1804, 2761
HapII CCGG 9 cut(s) 335, 386, 890, 1001, 1005, 1090, 2265, 2359, 2739
HgaI GACGC 2 cut(s) 1841, 2209
HhaI GCGC 4 cut(s) 898, 1297, 2077, 2158
Hin1I GRCGYC 1 cut(s) 2201
Hin6I GCGC 4 cut(s) 896, 1295, 2075, 2156
HinP1I GCGC 4 cut(s) 896, 1295, 2075, 2156
HincII GTYRAC 2 cut(s) 1636, 1666
HindII GTYRAC 2 cut(s) 1636, 1666
HindIII AAGCTT 1 cut(s) 2003
HpaII CCGG 9 cut(s) 335, 386, 890, 1001, 1005, 1090, 2265, 2359, 2739
HphI GGTGA 6 cut(s) 82, 148, 443, 833, 905, 2077
Hpy166II GTNNAC 6 cut(s) 759, 1199, 1246, 1357, 1636, 1666
Hpy188III TCNNGA 9 cut(s) 569, 1286, 1685, 1822, 1864, 2306, 2336, 2692, 2720
Hpy8I GTNNAC 6 cut(s) 759, 1199, 1246, 1357, 1636, 1666
Hpy99I CGWCG 4 cut(s) 637, 1637, 1667, 1835
HpyAV CCTTC 5 cut(s) 97, 135, 1618, 2646, 2848
HpyCH4III ACNGT 6 cut(s) 455, 559, 1054, 1406, 1663, 2735
HpyCH4IV ACGT 4 cut(s) 315, 487, 981, 1133
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 800, 2099
HpyF3I CTNAG 7 cut(s) 195, 804, 1739, 2079, 2127, 2750, 2816
HpySE526I ACGT 4 cut(s) 315, 487, 981, 1133
Hsp92I GRCGYC 1 cut(s) 2201
HspAI GCGC 4 cut(s) 896, 1295, 2075, 2156
KflI GGGWCCC 1 cut(s) 1839
KpnI GGTACC 1 cut(s) 1443
Ksp22I TGATCA 1 cut(s) 1288
KspI CCGCGG 1 cut(s) 1653
LmnI GCTCC 7 cut(s) 184, 861, 1460, 1554, 1607, 1649, 1733
Lsp1109I GCAGC 5 cut(s) 290, 893, 1939, 2785, 2799
LweI GCATC 1 cut(s) 1888
MaeI CTAG 7 cut(s) 423, 713, 725, 878, 920, 2319, 2469
MaeII ACGT 4 cut(s) 315, 487, 981, 1133
MbiI CCGCTC 1 cut(s) 2850
MboII GAAGA 4 cut(s) 1003, 1330, 1484, 1546
MfeI CAATTG 2 cut(s) 1141, 2661
MflI RGATCY 3 cut(s) 19, 1824, 2083
MhlI GDGCHC 3 cut(s) 217, 243, 724
MlsI TGGCCA 1 cut(s) 1448
MluNI TGGCCA 1 cut(s) 1448
MlyI GAGTC 7 cut(s) 649, 867, 966, 1002, 1682, 1697, 2725
MmeI TCCRAC 4 cut(s) 615, 2250, 2273, 2404
Mox20I TGGCCA 1 cut(s) 1448
MroXI GAANNNNTTC 4 cut(s) 1281, 1482, 2019, 2858
MscI TGGCCA 1 cut(s) 1448
MslI CAYNNNNRTG 3 cut(s) 921, 1970, 2457
Msp20I TGGCCA 1 cut(s) 1448
MspA1I CMGCKG 2 cut(s) 26, 1652
MspCI CTTAAG 1 cut(s) 1501
MspI CCGG 9 cut(s) 335, 386, 890, 1001, 1005, 1090, 2265, 2359, 2739
MspR9I CCNGG 8 cut(s) 227, 1001, 1048, 1091, 1789, 2266, 2359, 2740
MunI CAATTG 2 cut(s) 1141, 2661
Mva1269I GAATGC 3 cut(s) 1243, 2059, 2383
MvaI CCWGG 3 cut(s) 227, 1048, 1789
MvnI CGCG 4 cut(s) 896, 1652, 1830, 1835
MwoI GCNNNNNNNGC 3 cut(s) 284, 800, 2099
NciI CCSGG 5 cut(s) 1001, 1091, 2266, 2359, 2740
NcoI CCATGG 3 cut(s) 41, 1443, 1965
NdeI CATATG 2 cut(s) 1366, 2524
NheI GCTAGC 2 cut(s) 2318, 2468
NlaIV GGNNCC 7 cut(s) 180, 231, 1441, 1645, 1840, 1841, 2787
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 7 cut(s) 154, 425, 483, 641, 906, 2197, 2459
NspI RCATGY 1 cut(s) 1467
NspV TTCGAA 3 cut(s) 2192, 2375, 2639
PacI TTAATTAA 1 cut(s) 2148
PaeI GCATGC 1 cut(s) 1467
PagI TCATGA 1 cut(s) 2691
PcsI WCGNNNNNNNCGW 3 cut(s) 484, 2042, 2381
PctI GAATGC 3 cut(s) 1243, 2059, 2383
PdmI GAANNNNTTC 4 cut(s) 1281, 1482, 2019, 2858
PfeI GAWTC 8 cut(s) 1413, 1655, 2213, 2255, 2261, 2365, 2386, 2606
PflFI GACNNNGTC 1 cut(s) 956
PflMI CCANNNNNTGG 1 cut(s) 294
PfoI TCCNGGA 1 cut(s) 1046
Ple19I CGATCG 1 cut(s) 2481
PleI GAGTC 7 cut(s) 648, 867, 965, 1002, 1682, 1697, 2724
PpsI GAGTC 7 cut(s) 648, 867, 965, 1002, 1682, 1697, 2724
Ppu21I YACGTR 2 cut(s) 316, 488
PpuMI RGGWCCY 3 cut(s) 1497, 1839, 2633
PshBI ATTAAT 2 cut(s) 1872, 2298
Psp1406I AACGTT 2 cut(s) 981, 1133
Psp5II RGGWCCY 3 cut(s) 1497, 1839, 2633
Psp6I CCWGG 3 cut(s) 225, 1046, 1787
PspGI CCWGG 3 cut(s) 225, 1046, 1787
PspN4I GGNNCC 7 cut(s) 180, 231, 1441, 1645, 1840, 1841, 2787
PspPI GGNCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
PspPPI RGGWCCY 3 cut(s) 1497, 1839, 2633
PstNI CAGNNNCTG 1 cut(s) 1274
PsuI RGATCY 3 cut(s) 19, 1824, 2083
PsyI GACNNNGTC 1 cut(s) 956
PvuI CGATCG 1 cut(s) 2481
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 8 cut(s) 770, 1148, 1247, 1350, 1441, 1541, 1814, 1851
RsaNI GTAC 8 cut(s) 769, 1147, 1246, 1349, 1440, 1540, 1813, 1850
RseI CAYNNNNRTG 3 cut(s) 921, 1970, 2457
SacII CCGCGG 1 cut(s) 1653
SalI GTCGAC 2 cut(s) 1634, 1664
Sau96I GGNCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
SchI GAGTC 7 cut(s) 649, 867, 966, 1002, 1682, 1697, 2725
ScrFI CCNGG 8 cut(s) 227, 1001, 1048, 1091, 1789, 2266, 2359, 2740
SduI GDGCHC 3 cut(s) 217, 243, 724
SfaNI GCATC 1 cut(s) 1888
SfcI CTRYAG 1 cut(s) 1777
Sfr303I CCGCGG 1 cut(s) 1653
SfuI TTCGAA 3 cut(s) 2192, 2375, 2639
SgrBI CCGCGG 1 cut(s) 1653
SinI GGWCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
SmiMI CAYNNNNRTG 3 cut(s) 921, 1970, 2457
SmlI CTYRAG 2 cut(s) 1501, 2906
SmoI CTYRAG 2 cut(s) 1501, 2906
SphI GCATGC 1 cut(s) 1467
SspI AATATT 2 cut(s) 149, 1086
SspMI CTAG 7 cut(s) 423, 713, 725, 878, 920, 2319, 2469
StyD4I CCNGG 8 cut(s) 225, 999, 1046, 1089, 1787, 2264, 2357, 2738
StyI CCWWGG 4 cut(s) 41, 1394, 1443, 1965
TaaI ACNGT 6 cut(s) 455, 559, 1054, 1406, 1663, 2735
TaiI ACGT 4 cut(s) 318, 490, 984, 1136
TaqII GACCGA 2 cut(s) 969, 1955
TatI WGTACW 2 cut(s) 1245, 1348
TauI GCSGC 6 cut(s) 409, 1644, 1677, 1804, 2783, 2804
TfiI GAWTC 8 cut(s) 1413, 1655, 2213, 2255, 2261, 2365, 2386, 2606
TscAI CASTG 1 cut(s) 2738
TseFI GTSAC 7 cut(s) 154, 425, 483, 641, 906, 2197, 2459
TseI GCWGC 5 cut(s) 278, 881, 1952, 2798, 2812
Tsp45I GTSAC 7 cut(s) 154, 425, 483, 641, 906, 2197, 2459
TspGWI ACGGA 5 cut(s) 512, 1527, 2562, 2611, 2698
TspRI CASTG 1 cut(s) 2738
Tth111I GACNNNGTC 1 cut(s) 956
Van91I CCANNNNNTGG 1 cut(s) 294
Vha464I CTTAAG 1 cut(s) 1501
VpaK11BI GGWCC 7 cut(s) 45, 337, 952, 1497, 1839, 2333, 2633
VspI ATTAAT 2 cut(s) 1872, 2298
XapI RAATTY 6 cut(s) 502, 1175, 2015, 2131, 2530, 2858
XceI RCATGY 1 cut(s) 1467
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XmiI GTMKAC 5 cut(s) 758, 1198, 1356, 1635, 1665
XmnI GAANNNNTTC 4 cut(s) 1281, 1482, 2019, 2858
XspI CTAG 7 cut(s) 423, 713, 725, 878, 920, 2319, 2469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.