MD01G1080500.v1.1

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007431
Physical Location & Seq
Forward (+)
18770188 .. 18781501
11314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1080500.v1.1.491

Sequence Viewer

Length: 5610 bp
ATGGCGCCCAGAAACAGCCGTGGAAAGGGAAAAGGAGACAAGAAGAAGAAAGAAGAAAAGGTTCTTCCTGTTGTTATGGATATCACTGTGAACCTTCCTGATGAAAGCAGTGTCGTTTTGAAGGGAATATCGACAGATAAGATTATCGATGTTCGTCAGCTGTTATCAGTGAACACAGAAACTTGTAACATTACGAACTTTTCACTATCTCATGAGGTAAGAGGGCAACGATTGAAGGACACGGTGGACGTCTCCGCACTGAAGCCGTGCGTTCTGGCGTTGGTCGAAGAGGACTACGATGAACAGCGCGCCACGGCGCACGTTAGACGCGTGCTCGACATTGTTGCCTGCACGACAAGCTTCGGAGCGTCGTCTTTGCCGGCGAAGGACCAAAGATCGAAGCTCGATGCTTCTTCAACTGGCTCCGGCAAAAATGCTCCGGTTGCGCAGGATAAGATCTCCAAGAAATCCAACGCCACGACCGCCGCCGCCGTCAACGCCTCCAAGTCTCAAGTTCCTACCGGAACCGATAAGCGAGACGTGGCGGGGGACTCGGAGACCGAGATGAGCCACTCCTGCCTCAAGCTTGGTTCTTTCTACGACTTCTTCTCTCTCTCCCACCTCACTCCCCCTTTGCAATTTATTCGAAGGGCGGCGAAACGGCAAGTCGATGAGATTGCGGCGGATGATCACATTTTTTCTCTAGAAGTGAAGCTTTGCAATGGGAAGGTGCTTCTTGTTGAAGCTTGCCGAAAGGGGTTTTATAGCATAGGGAAGCAGCGGGTTCTCTGTCACAACCTCGTCGATTTGCTACGGCAGCTTAGTAGAGCCTTCGACAATGCTTATGATGAGCTCTTGAAAGCATTTGCAGAACGGAATAAGTTTGGGAACCTTCCTTATGGGTTTAGAGCAAACACGTGGCTCGTACCCCCTGTTTCAGCGCAGTCACCATCAGTTTTCCCTGCTCTCCCCGTGGAGGATGAAACTTGGGGTGGAAATGGTGGTGGTCTAGGAAGAGATGGGAAATTTGATCTGATCCCTTGGGCTAATGAGTTTTGGCATATTGCATCCATGCCTTGCAAGACAGCCGAGGAGAGGCAGATTCGTGATAGGAAAGCATTCCTTCTTCACAGCCTATTCGTCGATGTTTCCATTTTTAGAGCCATTAAGGCTGTGCAGCATGTAATGGGAAAACCGGAATTGACTTGTTCAGTTCCAAACAGTGAGATTCTTTACTCCAAGAGTGTAGGGGACTTGAATGTCACAGTTATGAAAGATGTTTCTAATGCAAGCTGTAAGGTAGATACTAAAATTGATGGAATTCAAGCAACTGGAGTGGACGAAGCGAATCTAGCGCAACGAAACCTTCTGAAAGGGATCACCGCAGATGAAAATACTGCTGCCCACGATGTCAACACTCTAGGTGTTGTCAATGTCAGATACTGTGGTTACATTGCAGTTGTCAAGGTTGAGGGGAAAGAAAATAAGAAAGTTGGTTCTCCATCTCAGAGCATTGAATTTGTTGATCAACCCGAAGGTGGTGCCAATGCCCTTAATATCAACAGTTTAAGATTACTACTTCACAAGACAATACCTTCAGAGCAGAATAAACCGGCATCACATATGCAAACTCTGGAACACGAAGATCTTTCAGCCTCATGTGTTTTTGTTGAGGGGGTGTTAGAAGAAAGTCTTGCTAAGCTTGAGAAAGAGGAGCTAGATTCTGATAATTTTGTGAGATGGGAACTTGGAGCTTGCTGGATACAACACTTGCAAGACCAAAAAAATGCAGACAAAGATAAGAAACCGTCAAACGAGAAGGCTAAAAATGAGTTGAAGGTTGAGGGACTTGGGACACCTCTTAAATCTCTTAAGAACAGCAAGAAAAAATCAGATGGAGGCAATACCAAGCTGCAATCTGAAAGCTCAAAGTCTCATGTAGATGGTGTTGTTGCGGAAGTTGAAAATAGTATATCCCCTTCTGTGGAATCAAAGCTTGAGACTAATGCCAAAGAAAATGAGCTTGTGTTGACAGAGATATTGTCTGATGCTGCCTTTGCTCGACTAAAAGATTCAGAAACTGGACTTCACTGCAAGTCTCTGCAAGAACTGATCGACTTGTCTCAGAAGTATTACTCTGAAGTTGCTCTTCCCAAACTGGTAGCAGATTTTGGTTCCTTGGAACTTTCACCGGTTGATGGTCGGACCTTGACTGATTTCATGCATACTAGAGGTCTTCGGATGCGCTCTCTGGGGAATGTTGTCAAGCTTTCGGAGAAGCTATCCCATGTGCAGTCCCTTTGCATTCATGAGATGATAGTGCGAGCTTTTAAGCACATACTTCAGGCAGTGATTGCTGCCGTTGGTAACACTGAGAAAATGGCTGTTTCAATTGCTGCTGCATTAAATCTTATGCTGGGGGTTTCTGATAATGAAGAATTAAATAAGTCTTGCAATGTTCATTCTCTTGTGTGGAAATGGCTGGAGGTATTCTTGCGAAAGAGATATGGATGGGATATTAACAGCTTCAACTATGACGATGTGAGGAGATTTGCGATTCTACGTGGATTATGTCACAAGGTGGGTATTGAGATGGTTCCAAGGGATTTTGATGTGGATTCTCCAAGTCCGTTCCAAAGTTCAGATATTGTCAGCCTAGTCCCAGTGCATAAGCAAGCTGCTTGCTCTTCGGCAGATGGTAGGCAACTCCTAGAATCATCAAAAACAGCTTTAGATAAGGGAAAACTTGAAGATGCAGTTGCATTTGGCACTAAGGCTCTTGCAAAGCTGGTAGCAGTTTGTGGTCCCTACCATCGAATGACAGCAGGAGCTTACAGCCTCCTTGCTGTAGTTTTATATCACACTGGAGACTTCAATCAGGCTACAATTTATCAGCAAAAGGCCCTGGATATCAACGAGAGAGAATTAGGGCTTGACCATCCAGACACCATGAAGAGTTATGGGGATCTTGCTGTGTTCTATTACAGACTTCAACATACGGAGCTGGCTCTCAAGTATGTAAAGCGTGCTCTGTATCTTTTGCATCTCACATGTGGACCATCTCATCCAAACACTGCTGCAACATACATCAATGTGGCTATGATGGAGGAAGGCCTGGGAAATGTGCATGTTGCCCTTCGATATCTACACAAAGCCTTGAAGTGTAATCAAAGGTTACTCGGCCCGGACCATATTCAGACAGCAGCAAGTTACCACGCTATTGCAATTGCTCTCTCTTTGATGGAAGCTTACCCTTTAAGTGTTCAGCATGAGCAGACAACTTTGCAAATTCTACGAGCAAAGCTTGGCCCAGATGACTTACGGACACAAGATGCTGCTGCTTGGCTTGAGTACTTTGAGTCCAAGGCTTTCGAACAGCAAGAAGCTGCAAGAAATGGGACTCGAAAGCCTGATGCATCTATTGCCAGCAAAGGCCACTTAAGTGTATCAGATTTGCTTGACTACATTAGTCCAGCTCATGGTGCCAAAGGGAGAGAGGTGGCAGGAAAGAGGAAAAGCTACTTAACAAAGTTGAAGGAAAAATCCATCCAAACTATCAGTTCAGCTAGTTCTGATGAGTCTTCAAAAGAAACCACAAAAGAGGGTTCGGACGAAGAGGGTCAGGAGACGAATGTACTTGAACCAATTGATAGAACAGATGTTATCCAGGAGAGCAGACCTCCTCTGGTTGAACCTCAGCACGTCGTGGAAGAGAATGCAGAGGAAAATTCAAATGTTTTCGATCAGATATCTTCTGAAACATATATCGAAGGTGGTGATGATGGGTGGCAATCAGTTCAAAGACCTAGATCAGCTGGCTCATATGGGCGGCGTCTAAAGCAGCGGCGGGCAACTATTGGGAAGGTCTATAATTACCAGAAGAAGTATGTTGAAAGTGACGTGGACTATTCTTCAGTGAAGAATATTAATCAAAACAGTAGTTATTATCTTGTAAAGAAAAGGCCAACATCTCACGGAAGTTATGCAGACAATCATACTGCAAAACCCTCCCAAGGTACTAAGTTTGGACGGAGAATAGTTAAAGGTGTAACATACAGAGTTAAGTCTATGCCTTCGTCTACCAAAGTAGATACCGAAGAGCCCTCTAACGGTGGCAAAATTTTGAGTTCTCCATCAGAATCTATTCAAAACGCTTCACCACATGGAATTGGCCCAGTAAAAAATTCCGTAGTCAGTCTTGGGAAGTCTCCTTCTTACAAGGAAGTAGCATTGGCTCCACCAGGTACTATTGGGAAATTTCAAACCCAGTACAACATTCCAGATAATCAGGAACATGGTGTCCGAGTTCATGAGGAAGAAACAACTGAAGTCAAAGGAGATTCCAAACCAAACACAACTGAGTTAGGAAATGTACTTGAAGAAAAGGACTCTGTGTTGGACTCTGTATTGGTTACGACAGACCACATACCAGACGAAACCGGAGCTGCTGAGAAGAAAGGAGAAGTTATTTCAAACGATTCAAAGGAAGATAAGTCTTCACTGATGGTATTAGAGAGTTTGGATGGACACGGATCCAGCGGTGTTAAGATTGATGAAGTGGTAGAAGATAACTTGTTGACAGACGGAGTGCCAAAATCTTTGGGTTCTCCCAAGGGATGTGAGACGGATCCATCTGGTACTTGTGAACTGCATGACTCCAACTCTACCATGCAAGGTGTAGACGATGCTGTGAGTTCAGTTGATACTCGGGGACTGCCCAGTAAGAAGCTATCTGCCTCAGCAGCTCCATTCAATCCCTCACCACCTGCTGCACGTGCTGCTCCGGTTTCCATGAACATTGCAATACCTTCGGGTGCGGGTAATGTCCCAACTGTTGCACCTTGGCCAGTAAACATGAATCTTCATCCAGGGCCCGGTACTGTCTTACCCACGGTCAATCCAATGTGCTCCTCCCCTCACCACCCATACCATTCTCCTCCTGCGACCCCAAACATCATACAACCATTGCCTTTTATGTACCCTCCTTACAGTCAACCCCAAGTAATCCGAACCAGTGCCTTTCCCATTACTAGCAGCGGGTTTCATCCAAATCATTTTGCATGGCAAAATGTGAACCCCCATGTTCCCGAGTTTGTTCATAGTCCAGTTTGGCCTGGTTGTCATCCAATGGATTTCTCTGCCCCTACACCTGTTGCTGAGCCAATTTCTGAACCGACAGTGGAGCCAAAATTTCACAATGATGATTCTGCTCCAGTTCTGCCAGCGGACATTGACAATTTGGAGGAAACTAAGCAAGAAGTGAACCTTCTGACGTCAGAGGCAATGAGCAATGCAGTTGAAAGTGTAAAGGAAAATGGTCCTTCAAACCTTTGCAGGGTTGAACTTGCTCAGAGCGAACCAACTGACAACCCAAATGGAAATGCTGCAAGTAGCGGTGAACGAACAAACGATGGAGAGAAAACCTTCAGCATTTTGTTGAGGGGTAGGAGAAACAGAAAGCAGACTCTGCGAATGCCAATCAGTTTGCTCAGTCGACCGTATGGCTCACAGTCCTTCAAAGTCATATGTAACAGAGTAGTCAGAGGGAATGATGCTACCAAAGCCACCAGCTTTTCATCAAGTGAAAATTGCACAGCTACTGCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1870

Amino Acids

204.24

Weight (kDa)

6.19

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 45 - 115 1.9e-11 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 729 - 871 6.5e-24 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 911 - 973 6.9e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 914 - 1012 1.8e-07 Nephrocystin-3 TPR domain
TPR_10 PF13374 941 - 982 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 941 - 1011 1.5e-12 Tetratricopeptide repeat
TPR_10 PF13374 1025 - 1065 1.7e-07 Tetratricopeptide repeat
TPR_12 PF13424 1025 - 1099 4.2e-12 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4774
AasI GACNNNNNNGTC 1 cut(s) 1259
AatII GACGTC 2 cut(s) 252, 5276
Acc16I TGCGCA 1 cut(s) 447
Acc36I ACCTGC 1 cut(s) 4774
AccB1I GGYRCC 3 cut(s) 4, 1541, 3482
AccB7I CCANNNNNTGG 1 cut(s) 3479
AccI GTMKAC 3 cut(s) 4079, 4680, 5494
AccII CGCG 2 cut(s) 309, 330
AclWI GGATC 7 cut(s) 1030, 1385, 2973, 4527, 4540, 4622, 4635
AcoI YGGCCR 1 cut(s) 4844
AcsI RAATTY 9 cut(s) 1025, 1320, 1517, 3288, 3727, 4119, 4183, 4256, 5189
AcuI CTGAAG 7 cut(s) 281, 1581, 2160, 2327, 3897, 4347, 5410
AcvI CACGTG 2 cut(s) 918, 4775
AcyI GRCGYC 4 cut(s) 5, 249, 3832, 5273
AdeI CACNNNGTG 2 cut(s) 2581, 3706
AflII CTTAAG 2 cut(s) 1871, 3439
AflIII ACRYGT 3 cut(s) 328, 915, 3050
AgeI ACCGGT 1 cut(s) 2191
AjiI CACGTC 3 cut(s) 541, 3703, 3901
AjnI CCWGG 6 cut(s) 2904, 3114, 3666, 4240, 4867, 5113
AleI CACNNNNGTG 1 cut(s) 3441
AloI GAACNNNNNNTCC 2 cut(s) 4284, 4316
Alw21I GWGCWC 4 cut(s) 336, 855, 3031, 4910
AlwI GGATC 7 cut(s) 1030, 1385, 2973, 4527, 4540, 4622, 4635
AlwNI CAGNNNCTG 4 cut(s) 1443, 2081, 5467, 5600
Ama87I CYCGRG 2 cut(s) 4707, 5087
ApaI GGGCCC 1 cut(s) 4875
ApoI RAATTY 9 cut(s) 1025, 1320, 1517, 3288, 3727, 4119, 4183, 4256, 5189
AseI ATTAAT 1 cut(s) 3927
AsiGI ACCGGT 1 cut(s) 2191
Asp700I GAANNNNTTC 4 cut(s) 60, 1118, 4143, 5423
AspLEI GCGC 8 cut(s) 7, 309, 311, 319, 448, 943, 1357, 2247
AsuC2I CCSGG 2 cut(s) 3185, 4875
AsuHPI GGTGA 8 cut(s) 939, 1372, 2181, 3788, 4149, 4752, 4910, 5408
AsuII TTCGAA 2 cut(s) 646, 3372
AvaI CYCGRG 2 cut(s) 4707, 5087
AvaII GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5318
BaeGI GKGCMC 1 cut(s) 4875
BaeI ACNNNNGTAYC 2 cut(s) 1432, 1465
BalI TGGCCA 1 cut(s) 4846
BamHI GGATCC 2 cut(s) 4532, 4627
BanI GGYRCC 3 cut(s) 4, 1541, 3482
BanII GRGCYC 3 cut(s) 855, 4104, 4875
BarI GAAGNNNNNNTAC 2 cut(s) 1963, 1995
BbrPI CACGTG 2 cut(s) 918, 4775
BbsI GAAGAC 3 cut(s) 2228, 3573, 4488
Bbv12I GWGCWC 4 cut(s) 336, 855, 3031, 4910
BbvCI CCTCAGC 2 cut(s) 3696, 4738
BceAI ACGGC 7 cut(s) 3, 250, 330, 476, 677, 830, 2345
BciT130I CCWGG 6 cut(s) 2906, 3116, 3668, 4242, 4869, 5115
BciVI GTATCC 1 cut(s) 1755
BclI TGATCA 2 cut(s) 688, 1525
BcnI CCSGG 2 cut(s) 3185, 4875
BfmI CTRYAG 1 cut(s) 2847
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 2 cut(s) 1871, 3439
BfuAI ACCTGC 1 cut(s) 4774
BfuI GTATCC 1 cut(s) 1755
BglI GCCNNNNNGGC 1 cut(s) 1169
BglII AGATCT 2 cut(s) 456, 1645
BlpI GCTNAGC 2 cut(s) 1698, 5157
BmcAI AGTACT 1 cut(s) 3353
Bme1390I CCNGG 8 cut(s) 2906, 3116, 3185, 3668, 4242, 4869, 4875, 5115
Bme18I GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5318
BmeT110I CYCGRG 2 cut(s) 4707, 5087
BmgBI CACGTC 3 cut(s) 541, 3703, 3901
BmrFI CCNGG 8 cut(s) 2906, 3116, 3185, 3668, 4242, 4869, 4875, 5115
BmrI ACTGGG 4 cut(s) 2657, 4169, 4261, 4713
BmuI ACTGGG 4 cut(s) 2657, 4169, 4261, 4713
BpiI GAAGAC 3 cut(s) 2228, 3573, 4488
BplI GAGNNNNNCTC 2 cut(s) 3664, 3696
BpmI CTGGAG 4 cut(s) 1353, 2504, 2886, 5196
Bpu10I CCTNAGC 2 cut(s) 3696, 4738
Bpu1102I GCTNAGC 2 cut(s) 1698, 5157
Bpu14I TTCGAA 2 cut(s) 646, 3372
BpuEI CTTGAG 6 cut(s) 495, 566, 1724, 2018, 2996, 3368
BpuMI CCSGG 2 cut(s) 3185, 4875
Bsa29I ATCGAT 1 cut(s) 147
BsaAI YACGTR 3 cut(s) 918, 2564, 4775
BsaHI GRCGYC 4 cut(s) 5, 249, 3832, 5273
BsaI GGTCTC 1 cut(s) 551
BsaWI WCCGGW 6 cut(s) 439, 521, 1195, 2191, 4439, 4783
Bse118I RCCGGY 3 cut(s) 379, 1612, 2191
Bse3DI GCAATG 7 cut(s) 727, 1452, 2461, 4797, 4964, 5289, 5296
BseBI CCWGG 6 cut(s) 2906, 3116, 3668, 4242, 4869, 5115
BseCI ATCGAT 1 cut(s) 147
BseMI GCAATG 7 cut(s) 727, 1452, 2461, 4797, 4964, 5289, 5296
BsePI GCGCGC 1 cut(s) 307
BseRI GAGGAG 6 cut(s) 1106, 1727, 2560, 3672, 4900, 4926
BseSI GKGCMC 1 cut(s) 4875
BseYI CCCAGC 1 cut(s) 2416
BsgI GTGCAG 4 cut(s) 334, 1196, 2312, 4755
Bsh1236I CGCG 2 cut(s) 309, 330
Bsh1285I CGRYCG 2 cut(s) 483, 5498
BshNI GGYRCC 3 cut(s) 4, 1541, 3482
BshTI ACCGGT 1 cut(s) 2191
BshVI ATCGAT 1 cut(s) 147
BsiEI CGRYCG 2 cut(s) 483, 5498
BsiHKAI GWGCWC 4 cut(s) 336, 855, 3031, 4910
BsiHKCI CYCGRG 2 cut(s) 4707, 5087
BsmBI CGTCTC 4 cut(s) 256, 531, 3620, 4616
BsmI GAATGC 4 cut(s) 1118, 2304, 3721, 5478
Bso31I GGTCTC 1 cut(s) 551
BsoBI CYCGRG 2 cut(s) 4707, 5087
Bsp119I TTCGAA 2 cut(s) 646, 3372
Bsp120I GGGCCC 1 cut(s) 4871
Bsp1286I GDGCHC 6 cut(s) 336, 855, 3031, 4104, 4875, 4910
Bsp1720I GCTNAGC 2 cut(s) 1698, 5157
BspDI ATCGAT 1 cut(s) 147
BspFNI CGCG 2 cut(s) 309, 330
BspHI TCATGA 3 cut(s) 211, 2308, 4309
BspMI ACCTGC 1 cut(s) 4774
BspPI GGATC 7 cut(s) 1030, 1385, 2973, 4527, 4540, 4622, 4635
BspQI GCTCTTC 3 cut(s) 2154, 2692, 4092
BspT104I TTCGAA 2 cut(s) 646, 3372
BspT107I GGYRCC 3 cut(s) 4, 1541, 3482
BspTI CTTAAG 2 cut(s) 1871, 3439
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 7 cut(s) 727, 1452, 2461, 4797, 4964, 5289, 5296
BsrFI RCCGGY 3 cut(s) 379, 1612, 2191
BssAI RCCGGY 3 cut(s) 379, 1612, 2191
BssHII GCGCGC 1 cut(s) 307
BssNI GRCGYC 4 cut(s) 5, 249, 3832, 5273
BssT1I CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4841
Bst2UI CCWGG 6 cut(s) 2906, 3116, 3668, 4242, 4869, 5115
Bst6I CTCTTC 8 cut(s) 282, 1009, 2154, 2692, 2948, 3609, 3705, 4092
BstACI GRCGYC 4 cut(s) 5, 249, 3832, 5273
BstAFI CTTAAG 2 cut(s) 1871, 3439
BstAPI GCANNNNNTGC 4 cut(s) 2354, 3422, 4778, 5467
BstBAI YACGTR 3 cut(s) 918, 2564, 4775
BstBI TTCGAA 2 cut(s) 646, 3372
BstDSI CCRYGG 4 cut(s) 19, 312, 972, 4890
BstFNI CGCG 2 cut(s) 309, 330
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 8 cut(s) 7, 309, 311, 319, 448, 943, 1357, 2247
BstMCI CGRYCG 2 cut(s) 483, 5498
BstNI CCWGG 6 cut(s) 2906, 3116, 3668, 4242, 4869, 5115
BstNSI RCATGY 3 cut(s) 1184, 3054, 3131
BstSCI CCNGG 8 cut(s) 2904, 3114, 3183, 3666, 4240, 4867, 4873, 5113
BstSFI CTRYAG 1 cut(s) 2847
BstSLI GKGCMC 1 cut(s) 4875
BstUI CGCG 2 cut(s) 309, 330
BstV2I GAAGAC 3 cut(s) 2228, 3573, 4488
BstX2I RGATCY 5 cut(s) 456, 1645, 2965, 4532, 4627
BstYI RGATCY 5 cut(s) 456, 1645, 2965, 4532, 4627
Bsu15I ATCGAT 1 cut(s) 147
BsuI GTATCC 1 cut(s) 1755
BsuTUI ATCGAT 1 cut(s) 147
BtgI CCRYGG 4 cut(s) 19, 312, 972, 4890
BtrI CACGTC 3 cut(s) 541, 3703, 3901
BtsI GCAGTG 4 cut(s) 115, 2089, 2355, 3072
BveI ACCTGC 1 cut(s) 4774
CaiI CAGNNNCTG 4 cut(s) 1443, 2081, 5467, 5600
CciI TCATGA 3 cut(s) 211, 2308, 4309
CfoI GCGC 8 cut(s) 7, 309, 311, 319, 448, 943, 1357, 2247
Cfr10I RCCGGY 3 cut(s) 379, 1612, 2191
ClaI ATCGAT 1 cut(s) 147
CseI GACGC 3 cut(s) 336, 357, 3821
CsiI ACCWGGT 1 cut(s) 4240
CspAI ACCGGT 1 cut(s) 2191
CspCI CAANNNNNGTGG 2 cut(s) 1317, 1352
DinI GGCGCC 1 cut(s) 6
DraIII CACNNNGTG 2 cut(s) 2581, 3706
DrdI GACNNNNNNGTC 1 cut(s) 1259
DseDI GACNNNNNNGTC 1 cut(s) 1259
EaeI YGGCCR 1 cut(s) 4844
Eam1104I CTCTTC 8 cut(s) 282, 1009, 2154, 2692, 2948, 3609, 3705, 4092
EarI CTCTTC 8 cut(s) 282, 1009, 2154, 2692, 2948, 3609, 3705, 4092
EciI GGCGGA 1 cut(s) 698
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4841
Eco147I AGGCCT 1 cut(s) 3114
Eco24I GRGCYC 3 cut(s) 855, 4104, 4875
Eco31I GGTCTC 1 cut(s) 551
Eco32I GATATC 4 cut(s) 82, 2911, 3143, 3750
Eco47I GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5318
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 7 cut(s) 281, 1581, 2160, 2327, 3897, 4347, 5410
Eco72I CACGTG 2 cut(s) 918, 4775
Eco88I CYCGRG 2 cut(s) 4707, 5087
EcoICRI GAGCTC 1 cut(s) 853
EcoO109I RGGNCCY 2 cut(s) 2902, 4871
EcoRI GAATTC 1 cut(s) 1320
EcoRII CCWGG 6 cut(s) 2904, 3114, 3666, 4240, 4867, 5113
EcoRV GATATC 4 cut(s) 82, 2911, 3143, 3750
EcoT14I CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4841
EcoT22I ATGCAT 2 cut(s) 2226, 3418
EcoT38I GRGCYC 3 cut(s) 855, 4104, 4875
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4841
Esp3I CGTCTC 4 cut(s) 256, 531, 3620, 4616
FauI CCCGC 5 cut(s) 538, 774, 3840, 4810, 5030
FauNDI CATATG 3 cut(s) 1623, 3823, 5525
FbaI TGATCA 2 cut(s) 688, 1525
FblI GTMKAC 3 cut(s) 4079, 4680, 5494
FriOI GRGCYC 3 cut(s) 855, 4104, 4875
FspI TGCGCA 1 cut(s) 447
GlaI GCGC 8 cut(s) 6, 308, 310, 318, 447, 942, 1356, 2246
GsaI CCCAGC 1 cut(s) 2420
GsuI CTGGAG 4 cut(s) 1353, 2504, 2886, 5196
HaeII RGCGCY 1 cut(s) 8
HgaI GACGC 3 cut(s) 336, 357, 3821
HhaI GCGC 8 cut(s) 7, 309, 311, 319, 448, 943, 1357, 2247
Hin1I GRCGYC 4 cut(s) 5, 249, 3832, 5273
Hin6I GCGC 8 cut(s) 5, 307, 309, 317, 446, 941, 1355, 2245
HinP1I GCGC 8 cut(s) 5, 307, 309, 317, 446, 941, 1355, 2245
HincII GTYRAC 6 cut(s) 496, 1414, 2031, 4578, 4994, 5495
HindII GTYRAC 6 cut(s) 496, 1414, 2031, 4578, 4994, 5495
HindIII AAGCTT 9 cut(s) 358, 584, 713, 744, 1700, 1994, 2267, 3246, 3302
HphI GGTGA 8 cut(s) 939, 1372, 2181, 3788, 4149, 4752, 4910, 5408
Hpy99I CGWCG 4 cut(s) 373, 806, 1145, 3707
HpyCH4IV ACGT 9 cut(s) 249, 321, 540, 917, 2563, 3702, 3900, 4774, 5273
HpySE526I ACGT 9 cut(s) 249, 321, 540, 917, 2563, 3702, 3900, 4774, 5273
Hsp92I GRCGYC 4 cut(s) 5, 249, 3832, 5273
HspAI GCGC 8 cut(s) 5, 307, 309, 317, 446, 941, 1355, 2245
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
Ksp22I TGATCA 2 cut(s) 688, 1525
LguI GCTCTTC 3 cut(s) 2154, 2692, 4092
MabI ACCWGGT 1 cut(s) 4240
MaeII ACGT 9 cut(s) 249, 321, 540, 917, 2563, 3702, 3900, 4774, 5273
MfeI CAATTG 3 cut(s) 2391, 3225, 3645
MflI RGATCY 5 cut(s) 456, 1645, 2965, 4532, 4627
MhlI GDGCHC 6 cut(s) 336, 855, 3031, 4104, 4875, 4910
MlsI TGGCCA 1 cut(s) 4846
MluI ACGCGT 1 cut(s) 328
MluNI TGGCCA 1 cut(s) 4846
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 8 cut(s) 545, 3368, 3394, 3587, 4382, 4394, 4649, 5458
MmeI TCCRAC 4 cut(s) 495, 2183, 4377, 4683
Mox20I TGGCCA 1 cut(s) 4846
Mph1103I ATGCAT 2 cut(s) 2226, 3418
MroNI GCCGGC 1 cut(s) 379
MroXI GAANNNNTTC 4 cut(s) 60, 1118, 4143, 5423
MscI TGGCCA 1 cut(s) 4846
MslI CAYNNNNRTG 5 cut(s) 1268, 1941, 3092, 3441, 5199
Msp20I TGGCCA 1 cut(s) 4846
MspA1I CMGCKG 7 cut(s) 160, 781, 3815, 3844, 4539, 5037, 5225
MspCI CTTAAG 2 cut(s) 1871, 3439
MspR9I CCNGG 8 cut(s) 2906, 3116, 3185, 3668, 4242, 4869, 4875, 5115
MunI CAATTG 3 cut(s) 2391, 3225, 3645
Mva1269I GAATGC 4 cut(s) 1118, 2304, 3721, 5478
MvaI CCWGG 6 cut(s) 2906, 3116, 3668, 4242, 4869, 5115
MvnI CGCG 2 cut(s) 309, 330
NaeI GCCGGC 1 cut(s) 381
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 3185, 4875
NdeI CATATG 3 cut(s) 1623, 3823, 5525
NgoMIV GCCGGC 1 cut(s) 379
NmeAIII GCCGAG 2 cut(s) 1114, 3159
NmuCI GTSAC 5 cut(s) 791, 945, 1261, 2573, 3896
NsbI TGCGCA 1 cut(s) 447
NsiI ATGCAT 2 cut(s) 2226, 3418
NspI RCATGY 3 cut(s) 1184, 3054, 3131
NspV TTCGAA 2 cut(s) 646, 3372
OliI CACNNNNGTG 1 cut(s) 3441
PagI TCATGA 3 cut(s) 211, 2308, 4309
PaqCI CACCTGC 1 cut(s) 4774
PauI GCGCGC 1 cut(s) 307
PceI AGGCCT 1 cut(s) 3114
PciI ACATGT 1 cut(s) 3050
PciSI GCTCTTC 3 cut(s) 2154, 2692, 4092
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PctI GAATGC 4 cut(s) 1118, 2304, 3721, 5478
PdiI GCCGGC 1 cut(s) 381
PdmI GAANNNNTTC 4 cut(s) 60, 1118, 4143, 5423
PflMI CCANNNNNTGG 1 cut(s) 3479
PfoI TCCNGGA 1 cut(s) 3666
PinAI ACCGGT 1 cut(s) 2191
PleI GAGTC 8 cut(s) 545, 3367, 3394, 3586, 4382, 4394, 4649, 5458
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 2 cut(s) 918, 4775
PmlI CACGTG 2 cut(s) 918, 4775
PpsI GAGTC 8 cut(s) 545, 3367, 3394, 3586, 4382, 4394, 4649, 5458
Ppu21I YACGTR 3 cut(s) 918, 2564, 4775
PscI ACATGT 1 cut(s) 3050
PshBI ATTAAT 1 cut(s) 3927
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 6 cut(s) 2904, 3114, 3666, 4240, 4867, 5113
PspCI CACGTG 2 cut(s) 918, 4775
PspFI CCCAGC 1 cut(s) 2416
PspGI CCWGG 6 cut(s) 2904, 3114, 3666, 4240, 4867, 5113
PspOMI GGGCCC 1 cut(s) 4871
PstNI CAGNNNCTG 4 cut(s) 1443, 2081, 5467, 5600
PsuI RGATCY 5 cut(s) 456, 1645, 2965, 4532, 4627
PteI GCGCGC 1 cut(s) 307
PvuII CAGCTG 2 cut(s) 160, 3815
RseI CAYNNNNRTG 5 cut(s) 1268, 1941, 3092, 3441, 5199
SacI GAGCTC 1 cut(s) 855
SalI GTCGAC 1 cut(s) 5493
SapI GCTCTTC 3 cut(s) 2154, 2692, 4092
ScaI AGTACT 1 cut(s) 3353
SchI GAGTC 8 cut(s) 545, 3368, 3394, 3587, 4382, 4394, 4649, 5458
ScrFI CCNGG 8 cut(s) 2906, 3116, 3185, 3668, 4242, 4869, 4875, 5115
SduI GDGCHC 6 cut(s) 336, 855, 3031, 4104, 4875, 4910
SexAI ACCWGGT 1 cut(s) 4240
SfcI CTRYAG 1 cut(s) 2847
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 2 cut(s) 646, 3372
SinI GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5318
SmiMI CAYNNNNRTG 5 cut(s) 1268, 1941, 3092, 3441, 5199
SmlI CTYRAG 8 cut(s) 510, 581, 1703, 1871, 1997, 3011, 3347, 3439
SmoI CTYRAG 8 cut(s) 510, 581, 1703, 1871, 1997, 3011, 3347, 3439
SseBI AGGCCT 1 cut(s) 3114
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 3925
SstI GAGCTC 1 cut(s) 855
StuI AGGCCT 1 cut(s) 3114
StyD4I CCNGG 8 cut(s) 2904, 3114, 3183, 3666, 4240, 4867, 4873, 5113
StyI CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4841
TaiI ACGT 9 cut(s) 252, 324, 543, 920, 2566, 3705, 3903, 4777, 5276
TaqII GACCGA 1 cut(s) 575
TatI WGTACW 4 cut(s) 3351, 3634, 4269, 4372
TauI GCSGC 6 cut(s) 488, 491, 656, 683, 3832, 3847
TseFI GTSAC 5 cut(s) 791, 945, 1261, 2573, 3896
Tsp45I GTSAC 5 cut(s) 791, 945, 1261, 2573, 3896
Van91I CCANNNNNTGG 1 cut(s) 3479
Vha464I CTTAAG 2 cut(s) 1871, 3439
VpaK11BI GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5318
VspI ATTAAT 1 cut(s) 3927
XapI RAATTY 9 cut(s) 1025, 1320, 1517, 3288, 3727, 4119, 4183, 4256, 5189
XbaI TCTAGA 1 cut(s) 703
XceI RCATGY 3 cut(s) 1184, 3054, 3131
XmiI GTMKAC 3 cut(s) 4079, 4680, 5494
XmnI GAANNNNTTC 4 cut(s) 60, 1118, 4143, 5423
ZraI GACGTC 2 cut(s) 250, 5274
ZrmI AGTACT 1 cut(s) 3353
Zsp2I ATGCAT 2 cut(s) 2226, 3418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.