MD07G1149400.v1.1

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007431
Physical Location & Seq
Forward (+)
21771157 .. 21782625
11469 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1149400.v1.1.491

Sequence Viewer

Length: 5592 bp
ATGGGGCCCAGAAACAGCCGTGGAAAGGGAAAAGGAGACAAGAAGAAGAAAGAAGAGAAGGTTCTTCCTGTTGTTATGGACATCACTGTGAAACTTCCTGATGAAAGCAGTGTCATTTTGAAGGGAATATCGACGGATATGATTATTGATGTTCGTCAGCTATTATCAGTAAATACAGACACTTGTAGTATTACTAATTTTTCACTATCTCATGAGGTAAGAGGGCATCGATTAAAGGACGCGGTGGACGTCTCCGCACTCAAGCCATGCGTCCTGACGTTGGTCGAAGAGGACTACGATGAACAGCGCGCCACGGCGCACGTTAGACGAGTGCTCGACATCGTTGCATGCACGACGAGCTTCGAAGCGTCGTCTTTGCCGGCGAAGGACCAAAGCCCTAAGCTCGATGCTTCTTCAACTGGCTCTGGTAAAAATGCGCCGGGTGTGCAGGATAAGAGCTTCAAGAAATCCAACGCCACGGCCGCCGCAGCCAAGGCCACCAAGTCTCAAGTTTCTACCGGAACCGATAAGCGAGACGTGGCGGTGGACTCCGAGACCGAGATGAGCAACTCCTGCCTCAAGCTTGGTTCTTTCTACGACTTCTTCTCTCTCTCCCACCTCACACCCCCTTTGCAATTTGTTCGAAGGGCGGCGAAACGGCAAGTCGATGAGATTGAGGCGGATGATCACCTTTTTTCTCTTGAAGTGAAGCTTTGCAATGGGAAGGTGGTTCTCGTTGAAGCTTGCCGAAAGGGGTTTTATAGCTTAGGGAAGCGGCGGATTCTGTGTCACAACCTCGTCGATTTGCTACGGCAGCTTAGCAGAGCCTTTGACAATGCTTATGATGAGCTCTTGAAAGCATTTTCGGAACGGAATAAGTTTGGGAATCTTCCTTATGGGTTTAGAGCAAACACGTGGCTCGTACCGCCTGTTTCAGCTCAGTCGCCTTCAGTTTTCCCTGCTCTCCCCGTGGAGGATGAAACTTGGGGCGGAAATGGAGGTGGTCTAGGAAGAGATGGGAAATTTGATCTAATCCCCTGGGCTAATGAGTTTTGGCATATTGCATCCATGCCTTGCAAGACAGCTGAGGAGAGGCAGATTCGTGATAGGAAGGCATTCCTTCTTCACAGCCTATTCGTCGATGTTTCCATTTGTAGAGCCATTAATGCTGTGCAGCGTGTAATGGGAAAACCGGAATTGACTTGTTCAATTCCAAACAGTGAGATTCTTTACTCCGAGGTTGTAGGGGACTTGAATGTCACAGTTATGAAAGATGTTACTAATGCAAGCTTTAAGGTAGATACTAAAATTGATGGAATTCAAGCAACTGGAGTGGATAAAACGAATCTAGCACAACGAAACCTTCTGAAAGGGATCACTGCAGATGAAAATACTGCTGCCCACGATGTCAACACTCTAGGTGTTGTCAATGTCAGATATTGTGGTTACATTGCAGTTGTCAAAGTTGAGGGTAAAGAAAATAAGAAAGTCGGTTCTCCATCTCAGAGCATTGTATTTGTTGATCAACCCGAAGGTGGTGCCAATGCCGTTAATATCAACAGTTTAAGATTACTACTTCACAAGACAATACCTTCAGAGCAGAATAAACCGGCATCACATATACAAACTCTGGAACACGAAGATCTTTCAGCCTCGTGTGTTTTTGTTGAGGGGGTGTTAGAAGAAAGTCTTGCTAAGCTTGAGAAAGAGGAGCTAGATTCTGATAATTTTGTGAGATGGGAACTAGGAGCTTGCTGGATACAACACTTGCAAGACCAAAAAAATGCAGACAAAGATAAGAAACCGTCAAACGAGAAGGGAAAAAATGATATGAAGGTGGAGGGACTTGGGACGCCTCTTAAATCCCTTAAGAACAGCAAGAAAAAATCAGATGGAGGCAATACCAAGCTGCAATCTGAAAGCTCAAAATCCCATGCAGATGGTGGTGTTGCGGAAGCTGAAAATACTATATCCCCTTCTGTGGAATCAAAGCTTGAGACTAATGCCAAAGAAAATGAGCATGTTCTGACAGAGATATTGTCTGATGCTGCCTTTGCTCGACTAAAAGATTCAGAAACTGGACTACACTGCAAGTCTCTGCAAGAACTAATCAACTTGTCTCAGAAGTACTACTCTGAAGTTGCTCTTCCCAAACTGGTAGCAGATTTTGGTTCCTTGGAACTTTCACCAGTTGATGGTCGGACCTTGACTGATTTCATGCATACTAGAGGTCTTCGGATGCGCTCTCTGGGGCATGTTGTCAAGCTTTCGGAGAAGCTATCTCATGTGCAGTCCCTTTGCATTCATGAGATGATAGTGCGAGCTTTTAAGCACATACTTCAGGCAGTGATTGCCGCCGTTGGTAGCACTGAGAAGATGGCTGTATCAATTGCTGCTGCATTGAATCTTATGCTGGGCGTTTTGGATAATGAAGAATTAAATAGGTCTTGCAGTGTTCATTCTCTTGTGCGGAAGTGGCTGGAGGTATTCTTGCGAAAAAGATACGGATGGGATCTTAACAGCTTCAACTATGAAGATGTGAGGAGATTTGCAATTCTGCGTGGATTATGTCACAAGGTGGGTGTTGAGATGGTTCCAAGGGATTTTGATATGGATTCTCCAAATCCGTTCCAAAGTTCAGATATCGTCAGCCTAGTCCCAGTGCATAAGCAAGCTGCTTGCTCTTCAGCAGATGGTAGGCAACTCCTAGAATCATCAAAAACAGCTTTAGATAAGGGAAAACTTGAAGATGCAGTTGCATTTGGCACTAAGGCGCTTGCAAAGCTGGTAGCAGTTTGTGGTCCATACCATCGAATGACAGCCGGAGCTTACAGCCTCCTTGCTGTAGTTTTATATCACACTGGAGACTTTAATCAGGCTACAATTTATCAGCAAAAGGCCTTGGATATCAACGAGAGAGAATTAGGGCTTGACCATCCAGACACCATGAAGAGTTATGGGGATCTTGCTGTGTTCTATTACAGACTTCAACATACGGAGCTGGCTCTCAAGTATGTAAAGCGTGCTTTGTATCTTTTGCATCTCACATGTGGACCATCTCATCCAAACACTGCTGCAACATATATCAATGTGGCTATGATGGAGGAAGGCCTGGGCAATGTGCATGTTGCCCTCAGATATCTGCACAAAGCTTTGAAGTGTAATCAAAGGTTACTCGGCCCGGACCATATTCAGACAGCAGCAAGTTACCACGCTATTGCAATTGCTCTCTCTTTGATGGAAGCTTACCCTTTAAGTGTTCAGCATGAGCAGACAACTTTGCAAATTCTGCGATCAAAGCTTGGCCCAGATGACTTACGAACACAAGATGCTGCTGCTTGGCTTGAGTACTTTGAGTCCAAGGCTTTCGAACAGCAAGAAGCTGCAAGAAATGGGACTCGAAAGCCTGATGCATCTATTGCCAGCAAAGGCCACTTAAGTGTATCAGATTTGCTGGACTACATTAGTCCCACCAAAGGGAGAGAGGTTGCAGGAAAGAGGAAAAGCTACTTAACAAAGTTGAAGGAAAAATCCATCCAAACTGTCAGCTCAGCTAGTTCCGATGAGTCTTCAAAAGAAACCACAAAAGAGGGTTCTGATGATGAGGGTCAGGAGACAAATGCACTTGAACCAAGTGATAGAACAGATGTAATCCAGGAGAACAGCTCTGCTCTGGTTGAACCTCAGCATGCCGTGGAAGAGATTGCAGAGGAAAATTCAAATGTTTTTGATCAGATATCTGCTGAAACATATATCGAAGGTGGTGATGATGGCTGGCAATCAGTTCACAGACCTAGATCAGCTGGCTCATATGGGCGGCATCTAAGGCAGCGGCGGGCAACTATTGGGAAAGTCTATAATTACCAGAAGAAATATGGTGAAAGTGACATGGACTGTTCTTCAGTGAAGAATACAAATCAAAACAGTAGGTATTATCTTGTAAAGAAAAGGCCAACATCTCATGGAAGTTATGCAGATAATCACACTGCAACCCCCTCCCAAGGTACTAAGTTTGGACGGAGAATAGTTAAAGGTGTAACATACAGAGTCAAGCCTATGCCTTCGTCTACCAAAGTAGATACCGAAGAGCCCTCTAATGGTAGCACAAGTTTGAGTTCTCCATCAGAACTCAGTCAAAATGTTTCACCGCATGTTGTTGGCCCAGTGAAAAATTCCAGAGTCAGTCTTGGGAAGTCTCCTTCTTACAAGGAAGTAGCATTGGCTCCACCAGGTACTATTGGGAAATTTCAAACCCAAAATAACATTCCAGATAATCAGGAACATGGTGTCCGAGCACAAGAAGAAGAAACAATTGAAGTCAAAGGAGATTCCAAACCAAACACAACTGAGTTAGAAAATATACTTGAAGAAAAGAACTCTGTGTTGGTTACGACGGACCACTTACCAGAGGAAACTGGAGCTGCTGAGAAGAAAGGAGAAGTTATTTCAAACGATGCAAAGGAAGATAAATCTTCACTGATGGTATTTGAAAGTTTGGATGGACATGGATCCAGCGGTAATAAGGTTGATGAAGCGGTAGAAGATAACTTATTGACAGACGGAGTGCCAAAATCTTTGGGTTCTCCCAAGAAGGGATTCTGTGAGACGGATCCATCTGGCACTTGTGAACTCCGCGACTCCAACTCTACCACGCAAGGTGTAGCCGATGCTGTGAGTTCGGTTGATACGGATCCATTGCCCAGTAAGAAGCTGTCTGCCTCAGCAGCTCCATTCAATCCCTCACCATCTGTTGCACGTGCTGCTCCGGTTTCCATGAACATTGCAATACCTTCGGGTGCAGGTAATGTCCCAATTGTTGCACCTTGGCCTGTAAACATGAATCTTCATCCAGGGCCTGGTACTGTCTTACCCACGGTCAATCCAATGTGCTCCTCCCCTCACCACCCATACCATTCTCCTCCTGCGACCCCGAACATCATACAACCATTGCCTTTTGTGTACCCTCCTTACAGTCAACCCCAAGTAATCCGAACCAGTTCCTTTCCTGTTACTAGTAGCGGGTTTCATCCAAATCATTTCACATGGCATAACATGAACCCCAAAGCACCTGAGTTTGTTCATTGTACAGTTTGGCCTGGCTGTCATCCGATGGATTTCTCTGCCCCTACACCTGTTGCTGAGACAATTTCTGAATTGACATCGGAGCCAAAATTTCACAGTGATGATTCTGCTCCAGTTCTGCCAGCGGACATTGAGAATGTGGAGGAAACTAAGCAAGAAGTGAACCTTCTGACATCAGAGGCAATCAGCAATGCAGTTGAAAGTGTAGAGGAAAATGGTCCTTCAAACCTTTGCGGGGTTGAACATGCTCAGAGTGAACCAAGTGAGAACCCAAATGGAAATGCTGCAAGTAGCGGTGAACTGACAAACGATGGAGAGAAAACCTTCAGCATTTTGATAAGGGGTAGGAGAAACCGAAAGGCGACTCTGCGAATGCCAATCAGTTTGCTCAGTCGACCGTATGGCTCACAGTCCTTCAAAGTCACATGTAACAGAGTAGTCAGAGGGAATGATGCTACCAAAGCCACCAGTTTTTCATCAAGTGAAAGTTGCACAGCTACTGCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1864

Amino Acids

203.53

Weight (kDa)

6.17

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 45 - 115 1.6e-09 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 728 - 870 1.6e-22 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 910 - 972 6.9e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 913 - 1010 2e-07 Nephrocystin-3 TPR domain
TPR_10 PF13374 940 - 981 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 940 - 1010 1.5e-12 Tetratricopeptide repeat
TPR_10 PF13374 1024 - 1064 1.7e-07 Tetratricopeptide repeat
TPR_12 PF13424 1024 - 1098 3.4e-12 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1256
AatII GACGTC 1 cut(s) 252
Acc36I ACCTGC 1 cut(s) 4790
AccB1I GGYRCC 1 cut(s) 1538
AccB7I CCANNNNNTGG 2 cut(s) 2195, 4856
AccI GTMKAC 2 cut(s) 4067, 5476
AccII CGCG 3 cut(s) 242, 309, 4635
AclWI GGATC 9 cut(s) 1382, 2520, 2970, 4503, 4516, 4604, 4617, 4685, 4698
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 7 cut(s) 1022, 1317, 3285, 3715, 4171, 4244, 5171
AcuI CTGAAG 7 cut(s) 933, 1578, 2157, 2324, 2670, 3885, 5392
AcvI CACGTG 2 cut(s) 915, 4757
AcyI GRCGYC 2 cut(s) 249, 1853
AdeI CACNNNGTG 1 cut(s) 2578
AfaI GTAC 8 cut(s) 924, 2129, 3350, 4006, 4234, 4861, 4961, 5086
AflII CTTAAG 2 cut(s) 1868, 3436
AflIII ACRYGT 3 cut(s) 912, 3047, 5507
AhlI ACTAGT 1 cut(s) 5012
AjiI CACGTC 1 cut(s) 538
AjnI CCWGG 7 cut(s) 1037, 3111, 3654, 4228, 4849, 4855, 5095
AjuI GAANNNNNNNTTGG 6 cut(s) 4248, 4280, 4367, 4399, 4580, 4612
AleI CACNNNNGTG 1 cut(s) 3438
AloI GAACNNNNNNTCC 2 cut(s) 4272, 4304
Alw21I GWGCWC 4 cut(s) 336, 852, 4297, 4892
AlwI GGATC 9 cut(s) 1382, 2520, 2970, 4503, 4516, 4604, 4617, 4685, 4698
AlwNI CAGNNNCTG 3 cut(s) 2078, 4856, 5582
ApaI GGGCCC 1 cut(s) 9
ApoI RAATTY 7 cut(s) 1022, 1317, 3285, 3715, 4171, 4244, 5171
AseI ATTAAT 1 cut(s) 1164
Asp700I GAANNNNTTC 4 cut(s) 1115, 4595, 4996, 5405
AspLEI GCGC 6 cut(s) 309, 311, 319, 439, 2244, 2776
AsuC2I CCSGG 2 cut(s) 441, 3182
AsuHPI GGTGA 8 cut(s) 680, 2178, 3776, 3890, 4137, 4734, 4892, 5390
AsuII TTCGAA 3 cut(s) 363, 643, 3369
AvaII GGWCC 7 cut(s) 388, 2202, 2801, 3053, 3184, 4396, 5300
BaeGI GKGCMC 1 cut(s) 9
BamHI GGATCC 3 cut(s) 4508, 4609, 4690
BanI GGYRCC 1 cut(s) 1538
BanII GRGCYC 3 cut(s) 9, 852, 4092
BauI CACGAG 1 cut(s) 1654
BbrPI CACGTG 2 cut(s) 915, 4757
BbsI GAAGAC 2 cut(s) 2225, 3561
Bbv12I GWGCWC 4 cut(s) 336, 852, 4297, 4892
BbvCI CCTCAGC 3 cut(s) 1086, 3684, 4720
BceAI ACGGC 8 cut(s) 3, 330, 495, 674, 827, 1532, 2342, 3677
BcgI CGANNNNNNTGC 8 cut(s) 326, 360, 623, 657, 1520, 1554, 4773, 4807
BciT130I CCWGG 7 cut(s) 1039, 3113, 3656, 4230, 4851, 4857, 5097
BciVI GTATCC 1 cut(s) 1752
BclI TGATCA 3 cut(s) 685, 1522, 3730
BcnI CCSGG 2 cut(s) 441, 3182
BcuI ACTAGT 1 cut(s) 5012
BfmI CTRYAG 2 cut(s) 1380, 2844
BfoI RGCGCY 1 cut(s) 2777
BfrI CTTAAG 2 cut(s) 1868, 3436
BfuAI ACCTGC 1 cut(s) 4790
BfuI GTATCC 1 cut(s) 1752
BglII AGATCT 1 cut(s) 1642
BlpI GCTNAGC 3 cut(s) 818, 1695, 3550
BmcAI AGTACT 2 cut(s) 2129, 3350
Bme1390I CCNGG 9 cut(s) 441, 1039, 3113, 3182, 3656, 4230, 4851, 4857, 5097
Bme18I GGWCC 7 cut(s) 388, 2202, 2801, 3053, 3184, 4396, 5300
BmgBI CACGTC 1 cut(s) 538
BmrFI CCNGG 9 cut(s) 441, 1039, 3113, 3182, 3656, 4230, 4851, 4857, 5097
BmrI ACTGGG 3 cut(s) 2654, 4157, 4695
BmuI ACTGGG 3 cut(s) 2654, 4157, 4695
BoxI GACNNNNGTC 1 cut(s) 281
BpiI GAAGAC 2 cut(s) 2225, 3561
BplI GAGNNNNNCTC 2 cut(s) 3650, 3682
BpmI CTGGAG 5 cut(s) 1350, 2501, 2883, 4437, 5178
Bpu10I CCTNAGC 5 cut(s) 399, 766, 1086, 3684, 4720
Bpu1102I GCTNAGC 3 cut(s) 818, 1695, 3550
Bpu14I TTCGAA 3 cut(s) 363, 643, 3369
BpuEI CTTGAG 7 cut(s) 245, 492, 563, 1721, 2015, 2993, 3365
BpuMI CCSGG 2 cut(s) 441, 3182
Bsa29I ATCGAT 1 cut(s) 229
BsaAI YACGTR 2 cut(s) 915, 4757
BsaBI GATNNNNATC 1 cut(s) 4689
BsaHI GRCGYC 2 cut(s) 249, 1853
BsaI GGTCTC 1 cut(s) 548
BsaWI WCCGGW 3 cut(s) 518, 1192, 4765
Bse118I RCCGGY 2 cut(s) 379, 1609
Bse3DI GCAATG 7 cut(s) 724, 1449, 3124, 4694, 4779, 4946, 5278
Bse8I GATNNNNATC 1 cut(s) 4689
BseBI CCWGG 7 cut(s) 1039, 3113, 3656, 4230, 4851, 4857, 5097
BseCI ATCGAT 1 cut(s) 229
BseJI GATNNNNATC 1 cut(s) 4689
BseMI GCAATG 7 cut(s) 724, 1449, 3124, 4694, 4779, 4946, 5278
BsePI GCGCGC 1 cut(s) 307
BseRI GAGGAG 5 cut(s) 1103, 1724, 2557, 4882, 4908
BseSI GKGCMC 1 cut(s) 9
BseX3I CGGCCG 1 cut(s) 480
BseYI CCCAGC 1 cut(s) 2413
BsgI GTGCAG 5 cut(s) 467, 1193, 2309, 3128, 4818
Bsh1236I CGCG 3 cut(s) 242, 309, 4635
Bsh1285I CGRYCG 2 cut(s) 483, 5480
BshNI GGYRCC 1 cut(s) 1538
BshVI ATCGAT 1 cut(s) 229
BsiEI CGRYCG 2 cut(s) 483, 5480
BsiHKAI GWGCWC 4 cut(s) 336, 852, 4297, 4892
BsiSI CCGG 8 cut(s) 380, 440, 519, 1193, 1610, 2823, 3182, 4766
BslFI GGGAC 8 cut(s) 1262, 1857, 1864, 2278, 2642, 3409, 3453, 4793
BsmBI CGTCTC 3 cut(s) 256, 528, 4598
BsmFI GGGAC 8 cut(s) 1262, 1857, 1864, 2278, 2642, 3409, 3453, 4793
BsmI GAATGC 3 cut(s) 1115, 2301, 5460
Bso31I GGTCTC 1 cut(s) 548
Bsp119I TTCGAA 3 cut(s) 363, 643, 3369
Bsp120I GGGCCC 1 cut(s) 5
Bsp1286I GDGCHC 6 cut(s) 9, 336, 852, 4092, 4297, 4892
Bsp1407I TGTACA 1 cut(s) 5084
Bsp1720I GCTNAGC 3 cut(s) 818, 1695, 3550
BspDI ATCGAT 1 cut(s) 229
BspFNI CGCG 3 cut(s) 242, 309, 4635
BspHI TCATGA 2 cut(s) 211, 2305
BspMAI CTGCAG 1 cut(s) 1384
BspMI ACCTGC 1 cut(s) 4790
BspPI GGATC 9 cut(s) 1382, 2520, 2970, 4503, 4516, 4604, 4617, 4685, 4698
BspQI GCTCTTC 3 cut(s) 2151, 2689, 4080
BspT104I TTCGAA 3 cut(s) 363, 643, 3369
BspT107I GGYRCC 1 cut(s) 1538
BspTI CTTAAG 2 cut(s) 1868, 3436
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 7 cut(s) 724, 1449, 3124, 4694, 4779, 4946, 5278
BsrFI RCCGGY 2 cut(s) 379, 1609
BsrGI TGTACA 1 cut(s) 5084
BssAI RCCGGY 2 cut(s) 379, 1609
BssHII GCGCGC 1 cut(s) 307
BssNI GRCGYC 2 cut(s) 249, 1853
BssSI CACGAG 1 cut(s) 1654
BssT1I CCWWGG 7 cut(s) 492, 2175, 2597, 2901, 3360, 4000, 4823
Bst2BI CACGAG 1 cut(s) 1654
Bst2UI CCWGG 7 cut(s) 1039, 3113, 3656, 4230, 4851, 4857, 5097
Bst6I CTCTTC 8 cut(s) 48, 282, 1006, 2151, 2689, 2945, 3693, 4080
BstACI GRCGYC 2 cut(s) 249, 1853
BstAFI CTTAAG 2 cut(s) 1868, 3436
BstAPI GCANNNNNTGC 5 cut(s) 573, 2351, 3289, 3419, 4760
BstAUI TGTACA 1 cut(s) 5084
BstBAI YACGTR 2 cut(s) 915, 4757
BstBI TTCGAA 3 cut(s) 363, 643, 3369
BstDSI CCRYGG 6 cut(s) 19, 312, 477, 969, 3693, 4872
BstFNI CGCG 3 cut(s) 242, 309, 4635
BstH2I RGCGCY 1 cut(s) 2777
BstHHI GCGC 6 cut(s) 309, 311, 319, 439, 2244, 2776
BstMCI CGRYCG 2 cut(s) 483, 5480
BstNI CCWGG 7 cut(s) 1039, 3113, 3656, 4230, 4851, 4857, 5097
BstNSI RCATGY 9 cut(s) 351, 2024, 2258, 3051, 3128, 3692, 4154, 5330, 5511
BstPAI GACNNNNGTC 1 cut(s) 281
BstSCI CCNGG 9 cut(s) 439, 1037, 3111, 3180, 3654, 4228, 4849, 4855, 5095
BstSFI CTRYAG 2 cut(s) 1380, 2844
BstSLI GKGCMC 1 cut(s) 9
BstUI CGCG 3 cut(s) 242, 309, 4635
BstV2I GAAGAC 2 cut(s) 2225, 3561
BstX2I RGATCY 6 cut(s) 1642, 2512, 2962, 4508, 4609, 4690
BstXI CCANNNNNNTGG 1 cut(s) 1940
BstYI RGATCY 6 cut(s) 1642, 2512, 2962, 4508, 4609, 4690
BstZI CGGCCG 1 cut(s) 480
Bsu15I ATCGAT 1 cut(s) 229
BsuI GTATCC 1 cut(s) 1752
BsuTUI ATCGAT 1 cut(s) 229
BtgI CCRYGG 6 cut(s) 19, 312, 477, 969, 3693, 4872
BtrI CACGTC 1 cut(s) 538
BtsI GCAGTG 7 cut(s) 115, 1377, 2086, 2352, 2458, 3069, 3984
BveI ACCTGC 1 cut(s) 4790
CaiI CAGNNNCTG 3 cut(s) 2078, 4856, 5582
CciI TCATGA 2 cut(s) 211, 2305
CfoI GCGC 6 cut(s) 309, 311, 319, 439, 2244, 2776
Cfr10I RCCGGY 2 cut(s) 379, 1609
ClaI ATCGAT 1 cut(s) 229
CseI GACGC 4 cut(s) 248, 259, 357, 1861
CsiI ACCWGGT 1 cut(s) 4228
Csp6I GTAC 8 cut(s) 923, 2128, 3349, 4005, 4233, 4860, 4960, 5085
CspCI CAANNNNNGTGG 2 cut(s) 1314, 1349
CviQI GTAC 8 cut(s) 923, 2128, 3349, 4005, 4233, 4860, 4960, 5085
DraIII CACNNNGTG 1 cut(s) 2578
DrdI GACNNNNNNGTC 1 cut(s) 1256
DseDI GACNNNNNNGTC 1 cut(s) 1256
EaeI YGGCCR 1 cut(s) 480
EagI CGGCCG 1 cut(s) 480
Eam1104I CTCTTC 8 cut(s) 48, 282, 1006, 2151, 2689, 2945, 3693, 4080
EarI CTCTTC 8 cut(s) 48, 282, 1006, 2151, 2689, 2945, 3693, 4080
EciI GGCGGA 3 cut(s) 695, 793, 1005
Ecl136II GAGCTC 1 cut(s) 850
EclXI CGGCCG 1 cut(s) 480
Eco130I CCWWGG 7 cut(s) 492, 2175, 2597, 2901, 3360, 4000, 4823
Eco147I AGGCCT 2 cut(s) 2900, 3111
Eco24I GRGCYC 3 cut(s) 9, 852, 4092
Eco31I GGTCTC 1 cut(s) 548
Eco32I GATATC 4 cut(s) 2644, 2908, 3140, 3738
Eco47I GGWCC 7 cut(s) 388, 2202, 2801, 3053, 3184, 4396, 5300
Eco52I CGGCCG 1 cut(s) 480
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 7 cut(s) 933, 1578, 2157, 2324, 2670, 3885, 5392
Eco72I CACGTG 2 cut(s) 915, 4757
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 2 cut(s) 5, 4853
EcoRI GAATTC 1 cut(s) 1317
EcoRII CCWGG 7 cut(s) 1037, 3111, 3654, 4228, 4849, 4855, 5095
EcoRV GATATC 4 cut(s) 2644, 2908, 3140, 3738
EcoT14I CCWWGG 7 cut(s) 492, 2175, 2597, 2901, 3360, 4000, 4823
EcoT22I ATGCAT 2 cut(s) 2223, 3415
EcoT38I GRGCYC 3 cut(s) 9, 852, 4092
ErhI CCWWGG 7 cut(s) 492, 2175, 2597, 2901, 3360, 4000, 4823
Esp3I CGTCTC 3 cut(s) 256, 528, 4598
FalI AAGNNNNNCTT 8 cut(s) 696, 728, 1674, 1706, 2130, 2162, 5232, 5264
FaqI GGGAC 8 cut(s) 1262, 1857, 1864, 2278, 2642, 3409, 3453, 4793
FauI CCCGC 3 cut(s) 3828, 5012, 5309
FauNDI CATATG 1 cut(s) 3811
FbaI TGATCA 3 cut(s) 685, 1522, 3730
FblI GTMKAC 2 cut(s) 4067, 5476
FriOI GRGCYC 3 cut(s) 9, 852, 4092
GlaI GCGC 6 cut(s) 308, 310, 318, 438, 2243, 2775
GsaI CCCAGC 1 cut(s) 2417
GsuI CTGGAG 5 cut(s) 1350, 2501, 2883, 4437, 5178
HaeII RGCGCY 1 cut(s) 2777
HapII CCGG 8 cut(s) 380, 440, 519, 1193, 1610, 2823, 3182, 4766
HgaI GACGC 4 cut(s) 248, 259, 357, 1861
HhaI GCGC 6 cut(s) 309, 311, 319, 439, 2244, 2776
Hin1I GRCGYC 2 cut(s) 249, 1853
Hin6I GCGC 6 cut(s) 307, 309, 317, 437, 2242, 2774
HinP1I GCGC 6 cut(s) 307, 309, 317, 437, 2242, 2774
HincII GTYRAC 3 cut(s) 1411, 4976, 5477
HindII GTYRAC 3 cut(s) 1411, 4976, 5477
HpaII CCGG 8 cut(s) 380, 440, 519, 1193, 1610, 2823, 3182, 4766
HphI GGTGA 8 cut(s) 680, 2178, 3776, 3890, 4137, 4734, 4892, 5390
Hpy99I CGWCG 6 cut(s) 136, 358, 373, 803, 1142, 4396
HpyCH4IV ACGT 6 cut(s) 249, 278, 321, 537, 914, 4756
HpySE526I ACGT 6 cut(s) 249, 278, 321, 537, 914, 4756
Hsp92I GRCGYC 2 cut(s) 249, 1853
HspAI GCGC 6 cut(s) 307, 309, 317, 437, 2242, 2774
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
Ksp22I TGATCA 3 cut(s) 685, 1522, 3730
LguI GCTCTTC 3 cut(s) 2151, 2689, 4080
MabI ACCWGGT 1 cut(s) 4228
MaeII ACGT 6 cut(s) 249, 278, 321, 537, 914, 4756
MfeI CAATTG 4 cut(s) 2388, 3222, 4311, 4812
MflI RGATCY 6 cut(s) 1642, 2512, 2962, 4508, 4609, 4690
MhlI GDGCHC 6 cut(s) 9, 336, 852, 4092, 4297, 4892
MlyI GAGTC 8 cut(s) 542, 3365, 3391, 3575, 4056, 4188, 4631, 5440
MmeI TCCRAC 3 cut(s) 495, 2180, 4665
Mph1103I ATGCAT 2 cut(s) 2223, 3415
MroNI GCCGGC 1 cut(s) 379
MroXI GAANNNNTTC 4 cut(s) 1115, 4595, 4996, 5405
MslI CAYNNNNRTG 5 cut(s) 86, 1265, 1938, 3438, 5181
MspA1I CMGCKG 5 cut(s) 1085, 3803, 3832, 4515, 5207
MspCI CTTAAG 2 cut(s) 1868, 3436
MspI CCGG 8 cut(s) 380, 440, 519, 1193, 1610, 2823, 3182, 4766
MspR9I CCNGG 9 cut(s) 441, 1039, 3113, 3182, 3656, 4230, 4851, 4857, 5097
MunI CAATTG 4 cut(s) 2388, 3222, 4311, 4812
Mva1269I GAATGC 3 cut(s) 1115, 2301, 5460
MvaI CCWGG 7 cut(s) 1039, 3113, 3656, 4230, 4851, 4857, 5097
MvnI CGCG 3 cut(s) 242, 309, 4635
NaeI GCCGGC 1 cut(s) 381
NciI CCSGG 2 cut(s) 441, 3182
NdeI CATATG 1 cut(s) 3811
NgoMIV GCCGGC 1 cut(s) 379
NmeAIII GCCGAG 1 cut(s) 3156
NmuCI GTSAC 5 cut(s) 788, 1258, 2570, 3884, 5503
NsiI ATGCAT 2 cut(s) 2223, 3415
NspI RCATGY 9 cut(s) 351, 2024, 2258, 3051, 3128, 3692, 4154, 5330, 5511
NspV TTCGAA 3 cut(s) 363, 643, 3369
OliI CACNNNNGTG 1 cut(s) 3438
PaeI GCATGC 2 cut(s) 351, 3692
PagI TCATGA 2 cut(s) 211, 2305
PasI CCCWGGG 1 cut(s) 1038
PauI GCGCGC 1 cut(s) 307
PceI AGGCCT 2 cut(s) 2900, 3111
PciI ACATGT 2 cut(s) 3047, 5507
PciSI GCTCTTC 3 cut(s) 2151, 2689, 4080
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PctI GAATGC 3 cut(s) 1115, 2301, 5460
PdiI GCCGGC 1 cut(s) 381
PdmI GAANNNNTTC 4 cut(s) 1115, 4595, 4996, 5405
PflMI CCANNNNNTGG 2 cut(s) 2195, 4856
PfoI TCCNGGA 1 cut(s) 3654
PleI GAGTC 8 cut(s) 542, 3364, 3391, 3574, 4055, 4187, 4631, 5440
PmaCI CACGTG 2 cut(s) 915, 4757
PmlI CACGTG 2 cut(s) 915, 4757
PpsI GAGTC 8 cut(s) 542, 3364, 3391, 3574, 4055, 4187, 4631, 5440
Ppu21I YACGTR 2 cut(s) 915, 4757
PscI ACATGT 2 cut(s) 3047, 5507
PshAI GACNNNNGTC 1 cut(s) 281
PshBI ATTAAT 1 cut(s) 1164
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 7 cut(s) 1037, 3111, 3654, 4228, 4849, 4855, 5095
PspCI CACGTG 2 cut(s) 915, 4757
PspFI CCCAGC 1 cut(s) 2413
PspGI CCWGG 7 cut(s) 1037, 3111, 3654, 4228, 4849, 4855, 5095
PspOMI GGGCCC 1 cut(s) 5
PstI CTGCAG 1 cut(s) 1384
PstNI CAGNNNCTG 3 cut(s) 2078, 4856, 5582
PsuI RGATCY 6 cut(s) 1642, 2512, 2962, 4508, 4609, 4690
PteI GCGCGC 1 cut(s) 307
PvuII CAGCTG 2 cut(s) 1085, 3803
RsaI GTAC 8 cut(s) 924, 2129, 3350, 4006, 4234, 4861, 4961, 5086
RsaNI GTAC 8 cut(s) 923, 2128, 3349, 4005, 4233, 4860, 4960, 5085
RseI CAYNNNNRTG 5 cut(s) 86, 1265, 1938, 3438, 5181
SacI GAGCTC 1 cut(s) 852
SalI GTCGAC 1 cut(s) 5475
SapI GCTCTTC 3 cut(s) 2151, 2689, 4080
ScaI AGTACT 2 cut(s) 2129, 3350
SchI GAGTC 8 cut(s) 542, 3365, 3391, 3575, 4056, 4188, 4631, 5440
ScrFI CCNGG 9 cut(s) 441, 1039, 3113, 3182, 3656, 4230, 4851, 4857, 5097
SduI GDGCHC 6 cut(s) 9, 336, 852, 4092, 4297, 4892
SexAI ACCWGGT 1 cut(s) 4228
SfcI CTRYAG 2 cut(s) 1380, 2844
SfuI TTCGAA 3 cut(s) 363, 643, 3369
SinI GGWCC 7 cut(s) 388, 2202, 2801, 3053, 3184, 4396, 5300
SmiMI CAYNNNNRTG 5 cut(s) 86, 1265, 1938, 3438, 5181
SmlI CTYRAG 9 cut(s) 260, 507, 578, 1700, 1868, 1994, 3008, 3344, 3436
SmoI CTYRAG 9 cut(s) 260, 507, 578, 1700, 1868, 1994, 3008, 3344, 3436
SpeI ACTAGT 1 cut(s) 5012
SphI GCATGC 2 cut(s) 351, 3692
SseBI AGGCCT 2 cut(s) 2900, 3111
SstI GAGCTC 1 cut(s) 852
StuI AGGCCT 2 cut(s) 2900, 3111
StyD4I CCNGG 9 cut(s) 439, 1037, 3111, 3180, 3654, 4228, 4849, 4855, 5095
StyI CCWWGG 7 cut(s) 492, 2175, 2597, 2901, 3360, 4000, 4823
TaiI ACGT 6 cut(s) 252, 281, 324, 540, 917, 4759
TaqII GACCGA 1 cut(s) 572
TatI WGTACW 3 cut(s) 2127, 3348, 5084
TauI GCSGC 7 cut(s) 485, 488, 653, 778, 2357, 3820, 3835
TseFI GTSAC 5 cut(s) 788, 1258, 2570, 3884, 5503
Tsp45I GTSAC 5 cut(s) 788, 1258, 2570, 3884, 5503
Van91I CCANNNNNTGG 2 cut(s) 2195, 4856
Vha464I CTTAAG 2 cut(s) 1868, 3436
VpaK11BI GGWCC 7 cut(s) 388, 2202, 2801, 3053, 3184, 4396, 5300
VspI ATTAAT 1 cut(s) 1164
XapI RAATTY 7 cut(s) 1022, 1317, 3285, 3715, 4171, 4244, 5171
XceI RCATGY 9 cut(s) 351, 2024, 2258, 3051, 3128, 3692, 4154, 5330, 5511
XcmI CCANNNNNNNNNTGG 2 cut(s) 1940, 3872
XmiI GTMKAC 2 cut(s) 4067, 5476
XmnI GAANNNNTTC 4 cut(s) 1115, 4595, 4996, 5405
ZraI GACGTC 1 cut(s) 250
ZrmI AGTACT 2 cut(s) 2129, 3350
Zsp2I ATGCAT 2 cut(s) 2223, 3415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.