Rroxscaffold_4G00297010

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007431
Physical Location & Seq
Reverse (-)
16996535 .. 17008623
12089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00297010.1

Sequence Viewer

Length: 5601 bp
ATGGCACCCAGAAACAGTCGTGGAAAGGGCAAAGGTGGAGAGAAGAAGAAGAAGGAAGAGAAGGTTCTTCCTGTTGTGATGGACATCACTGTGAACCTTCCAGATGAAAGCGGTGTTGTTTTGAAGCAGGGAATATCGACGGACAGGATTATCGACGTTCGCCAACTATTATCAGTCAACACAGAAACTTGTAACATCACCAATTTTTCACTATCCCATGAGGTAAGAGGGAAACGTTTAAAAGATACGGTGGACGTCTCCGCACTCAAGCCATGCCTCATCACCTTGGTCGAAGAGGACTATGATGAAGAGCGCGCCACCGCGCACGTGCGGCGCGTGCTGGACATCGTGGCCTGCACGACGAGCTTTGGCGCGTCACCGACGCCTCCGCCGCCGATTCCGGCGAAGGACCAAAGCCTCAAGCCCGACGCCTCGTCCGCAGTCCTCTCCGGTAAAAATGCGCAGGATAATAAGACCGCCAAGAAATCCACCGCCACCGCCACCGCCACAGCCGCCTCGGCCAAGCCTCAAGGCTCTGCCGGAGCCGATAATCGTGACGTGGCGGTCGACTCGGACGCCGAGCTGAGCCACTCTTGCCTCAAGCTCGGTTCTTTCTACGACTTCTTCTCTCTCTCTCATCTCACTCCCCCTCTGCAATTTATCCGAAGGGTGACGAAACGACATGTCGATGAGATTTCCGGCGATGATCACCTGTTTTCTCTTGAAGTGAAGCTTTGCAATGGGAAGGTGGTTCTGGTTGAAGCTTGCAGAAAGGGATTCTATAGTGTAGGGAAGCAGCGCATTCTGTGTCACAACCTCGTCGATTTGCTACGCCAGCTTAGTAGAGCTTTTGACAATGCTTACGATGATCTGCTGAAAGCGTTTTCGGAACGTAATAAGTTTGGGAATCTTCCCTATGGCTTTCGAGCAAACACATGGCTTGTACCGCCTATTGCAGGGCAGTCGCCTTCAGGTTTCCCTGCTCTTCCTGTGGAGGATGAAGCTTGGGGAGGAAATGGAGGGGGTCTAGGAAGGGATGGGCAATTCGATCTGATTCCTTGGGCTAATGAGTTTTGGTATATTGCATCTATGCCTTGCAAGACGGCGGAGGAGAGGCAGGTTCGAGATAGGAAGGCATTCCTTCTTCACAGCCTATTTGTCGACGTTTCCATTTTTAGAGCCATTAAGGCTGTGCAGCATGTGATGGCAAAGCCGGAATTAACATGTTCAGCTGCAAACAGTGAGGTTCTTTATACTGAGAGAGTAGGGGACCTGAATGTGATAGTAACAAAGGATGTTAACAATGCGAGCTGTAAGGTAGATACTAAAATTGATGGAATTCAGGCAACTGGAGTGGATAAAAAGAATCTAGCACAACGAAACCTTCTTAAAGGGATCACTGCTGACGAGAACACCGCTGCACATGATGTCACCACTCTAGGTGTTGTCAATGTCCGATATTGTGGTCACATTGCAGTTGTGAAAGTTGAGGGCAAAGAAACTAAGAAAGTCAGTTCTCCGTCCCAAAGCATTGACCTCCTTGATCAACCTGAAGGTGGTGCCAATGCCCTTAATATCAACAGTTTGAGATTACTACTTCACACTACTACACCTTCAGAGCAGAATAAACTGGCATCACATGGGCAAGGTCTGGAACATGAAGAGCTTTCTGCTTCCTGCGCTTTTGTTGAGAAGCTATTCGAAGAAAGTCTTGCTAAGCTTGATAAAGAGGAGCTAGATTCTGATAGTTTTGTGAGATGGGAACTAGGAGCTTGCTGGATACAACACTTGCAAGACCAAAAAAATACAGACAAGGATAAGAAACCATTGGGTGAGAAGGCTAAGAATGAGATGAAGGTCGAGGGACTTGGGACACCTCTTAAATCCCTTAAGAACACGAAGAAGAAATCAGATGGAGCCAATATTAAAGTGCAGTCTGATAGCTCAAAGTCGCATGCAGATGGTGTTGTGGGGGAAGTAGAAAATGCTATAGGTTCTGTGGAATCAAAGTTTGAAACCAGTGCTAAAGAAAATGAGCTTGTGCTGACAGAGATGCTCTCTGACGCAGCCTTTGCTCGACTAAAAGAATCAGAAACTGGACTTCATTGCAAGTCTCTGCAAGAACTGATCGACTTATCTCAGAAGTATTACTCTGAAGTTGCTCTTCCAAAACTGGTAGCCGATTTTGGTTCATTGGAACTTTCACCAGTTGATGGTCGAACTTTAACTGATTTCATGCATACTAGAGGTCTTCGGATGCGTTCTCTGGGGCAAGTTGTCAAGCTATCGGAGAAGCTATCACATGTGCAGTCCCTTTGCATTCATGAGATGATAGTGCGAGCTTTTAAGCACATACTTCAGGCAGTGATTGCTGCTGTTAATAACACTGAGAAAATGGCTGCTTCGATTGCTGCTGCATTGAATCTTATGTTGGGGGCTCCAGAAAATGAAGAATTCAATAAGTCTTTCAATGTGCATTCCCTTGTGTGGAGATGGCTGGAAGTATTCTTGCGAAAGAGATATGGATGGGATGTTAGCAGCTTCAACTACAATGATGTGAGGAGATTTGCGATTCTACGTGGCCTGTGTCATAAGGTGGGCATTGAGATGGTTCCACGAGATTTTGATATGGAGTCTCCAAATCCATTTCAGAGTTCTGATATTGTCAGCTTAGTTCCAGTACATAAGCAAGCTGCATGCTCTTCGGCAGATGGTAGGCAACTATTGGAGTCATCAAAAACAGCCTTAGATAAGGGAAAACTTGAAGATGCAGTTGCATATGGCACTAAGGCTCTTGCAAAGCTGGTAGCTGTTTGTGGTCCATACCATCGAATGACAGCAGGAGCTTACAGCCTCCTTGCTGTAGTTTTATATCACACTGGAGATTTTAATCAGGCTACAATTTATCAGCAAAAGGCTCTGGATATCAATGAGAGAGAATTAGGGCTTGACCATCCAGACACCATGAAGAGTTATGGGGATCTTGCTGTGTTCTATTACAGACTTCAACATACAGAGCTGGCTCTCAAATATGTAAAACGTGCTCTGTATCTCTTGCATCTCACTTGTGGACCATCTCATCCAAACACTGCTGCAACATACATCAACGTGGCTATGATGGAAGAAGGCCTGGGAAATGTACATGTTGCCCTCAGATATCTACACAAGGCTTTGAAGTGTAATCAGAAGTTACTTGGCCCAGATCATATTCAGACAGCGGCTAGTTACCATGCTATTGCAATTGCACTCTCATTGATGGAAGCATACCCTTTAAGTGTTCAGCATGAGCAGACAACATTGCAAATTCTGCGTGCAAAGCTTGGACCAGATGATCTACGTACACAAGATGCTGCTGCCTGGCTTGAGTACTTTGAGTCCAAGGCTTTTGAACAGCAAGAAGCTGCAAGAAATGGGACTCGAAAACCAGATGCATCTATAGCCAGCAAAGGCCACTTAAGCGTATCAGATTTGCTTGACTACATCAATCCCACTCATGATTCTAAAGGGAGAGATGCGGCAATGAAGAGGAAAACCTACATAACAAAGTTGAAGGAAAAATCCTATCAAACTATCAGCTCAGCAAGTTCTGACGAATCTTCGAAAGAAACCACAAAAGAGGTTTCAGATGAAGAAACACATACACTTGAACCAAGAGAAAGAACAGATCCCATCCAAGAAAACATTCCGGCTTCAGTTGAACCCCAGCATGTTGTAGAAGAGATTCCTGAGGAAAATTCAAACATGAGCTCTTCTGAAACACATGTTGAAGGGGAAGATGACTGGCAACCAGTTCAAAGGCCTAGATCAGCGGGTTCATATGGGCGGCGTGTAAAGCAGCGGCGGGCAGCTATTGGGAAGGTTTATAGTTACCAGAAGAAATATGTAGACGCTGATATGGATTACCCTTCAGTGAAGAATACTAACCAAAACAGTAGGTATTACCTTCTAAAGAAAAGGCCAACATCTCATGGAAGTTATACAGAAAATCAGATTGCGAATCCCTCCCAAGGTACTAAATTTGGACGGAGAATGGTAAAAGCTGTAGCATACAGAGTCAAGTCTATGCCTTTGTCTACCAAAGTAGTTGCAGCAGAAAGTTTGAGTTCCTCATCAGAAGTCAGTCCAAATGTTTCACCGCATGGGATTGGTCCAGTTAAAGGTTCTATAGTCAGTCTTGGAAAATCTCCTTCATACAAGGAAGTAGCATTGGCTCCACCAGGTAGTATTGCTAAAATGCAAACTGTTTTCCCTCAGAATGGCATTCCAGATAACCATGAGCATGGTATCCAAAGACAAGAAGAGGAAATAATTGAAATCAAAGGAGATTCCAAACCAAAGGCTGTAGTTGAAACTATACTTGAAGAAAAGAAGGATCCTGTATTGGTTTCCACAGATGTGTTACGAGAGCAAACTGGACCTGTTGAGAAGAAAGAAGATACTAATTCACCTGAAGTGAAGGAAGATAAAAATTCACTGATATTCGGGACCGTGGATGAACTTGGATCCAGTGCTGTTAAGTTTCAAGAGGCAGCAGTTGATAACGTATTAGTAGATGGACTGCCAAAATCCATTGATTCTGCCAAGGAGGGACTCTGTGAGAACAATCCATCTGGCACTTCTGAATTGCATGATTCCAACTCCACTTTGCAAGGAGTAGATGATTTGGAGGCATCCTCGCTTGTTACTTCAGCTGATACTCGGGGATTGCCAAATAAGAAGTTATCTGCATCGGCAGCTCCATTCAATCCCTCACCATCCACTCGTGCTGCTCCGGTTCCCATGAACATTGCTATACCTTCTGGTGCGGGTTCTGTCCCAACTATTGCACCTTGGCCGGTAAACCTGAATATTCACCCAGGGGCAGCTACTGTCTTACCCACAGTTAATCCCATGTGCTCCTCTCCTCATCATCCATACCCTTCTCCCCCTGCTACCCCGAATATCATACAGCCATTGCCATTTATGTTCCCTCCTCCTTACAGTCAACCCCAAGTTGTACGAACCAGTACCTTTCCTGTGACTAGTGCGTTTCATCCAAATCATTACACATGGCAGTGCAATGTGAATCCTAATGTATCCGAGTTTGTACCTACAACAGTTTGGCATGGTTGCCATCCAATGGAATTCTCTGCCCCAGCACCTGTTGTTGAGCCGATTTCTGATCCCTCTCCCTTGGAGTCAAACATTCAGAATGATGATTCTGCTCCAGTTCTGCCAGTGGACATTGATAATATGGGGGAAACTAAGAAAGAAGCAGACATTCTAACATCAGAGGCAACTTGTAATCCAATTGAAAGTGTCAAGGAAGTGGGTCCAGATCTTTGTGGGGTTGAGAATGCTCAGAACGAACCATGTGACAGCCCAAATGGAAAAGTTGGAAGTAGCGCTGAAAGGATGACTGGTGGAGAGAAAACCTTCAGCATTTTGATGAGGGGCAGAAGAAACCGAAAGCAGACTCTGAGAATGCCAGTAAGTTTGCTCAGTCGACCATATGGCTCACAGTCATTCAAAGTCATATATAACAGGGTGGTCAGGGGGAATGATGCGCCCAAACCCATCAGCTTTTCACCAAGTGAAAACTGCACAGCTACTGCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1866

Amino Acids

204.3

Weight (kDa)

6.3

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 47 - 117 2.2e-10 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 734 - 876 2.5e-24 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 916 - 978 2.3e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 918 - 1016 1.8e-07 Nephrocystin-3 TPR domain
TPR_10 PF13374 946 - 987 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 946 - 1016 1.5e-12 Tetratricopeptide repeat
TPR_10 PF13374 1030 - 1070 3.4e-07 Tetratricopeptide repeat
TPR_12 PF13424 1030 - 1104 5.3e-12 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 563
AatII GACGTC 1 cut(s) 258
Acc16I TGCGCA 1 cut(s) 462
Acc36I ACCTGC 1 cut(s) 1108
AccB1I GGYRCC 2 cut(s) 4, 1559
AccB7I CCANNNNNTGG 2 cut(s) 2213, 5119
AccI GTMKAC 5 cut(s) 567, 1161, 3885, 4073, 5485
AccII CGCG 4 cut(s) 315, 323, 336, 374
AclI AACGTT 1 cut(s) 235
AclWI GGATC 8 cut(s) 1403, 2988, 3659, 4367, 4380, 4497, 4510, 5156
AcoI YGGCCR 2 cut(s) 519, 4832
AcsI RAATTY 7 cut(s) 1338, 2453, 3303, 3733, 4016, 4468, 5123
AcvI CACGTG 1 cut(s) 328
AcyI GRCGYC 4 cut(s) 255, 383, 429, 576
AdeI CACNNNGTG 1 cut(s) 5573
AfaI GTAC 9 cut(s) 945, 2682, 3141, 3340, 3368, 4012, 4998, 5008, 5088
AfeI AGCGCT 1 cut(s) 5386
AfiI CCNNNNNNNGG 8 cut(s) 956, 1556, 2213, 2487, 4007, 4157, 4256, 5119
AflII CTTAAG 2 cut(s) 1889, 3454
AflIII ACRYGT 5 cut(s) 682, 1223, 2302, 3142, 3760
AhdI GACNNNNNGTC 1 cut(s) 433
AhlI ACTAGT 1 cut(s) 5021
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 4 cut(s) 3129, 3356, 4216, 4855
AjuI GAANNNNNNNTTGG 4 cut(s) 2414, 2446, 3666, 3698
AleI CACNNNNGTG 1 cut(s) 4394
Alw21I GWGCWC 3 cut(s) 3046, 3749, 4898
Alw26I GTCTC 3 cut(s) 262, 2118, 2640
AlwI GGATC 8 cut(s) 1403, 2988, 3659, 4367, 4380, 4497, 4510, 5156
AlwNI CAGNNNCTG 5 cut(s) 2096, 4868, 5141, 5458, 5591
Ama87I CYCGRG 1 cut(s) 4698
Aor51HI AGCGCT 1 cut(s) 5386
AoxI GGCC 9 cut(s) 351, 519, 2581, 3127, 3196, 3448, 3797, 3956, 4832
ApoI RAATTY 7 cut(s) 1338, 2453, 3303, 3733, 4016, 4468, 5123
ArsI GACNNNNNNTTYG 2 cut(s) 2054, 2086
Asp700I GAANNNNTTC 5 cut(s) 1136, 1697, 3681, 3720, 5414
AsuII TTCGAA 2 cut(s) 1701, 3599
AvaI CYCGRG 1 cut(s) 4698
AvaII GGWCC 9 cut(s) 409, 1270, 2819, 3071, 3323, 4148, 4415, 4485, 5312
AxyI CCTNAGG 1 cut(s) 3726
BamHI GGATCC 2 cut(s) 4372, 4502
BanI GGYRCC 2 cut(s) 4, 1559
BanII GRGCYC 2 cut(s) 2440, 3749
BauI CACGAG 2 cut(s) 2616, 4761
BbrPI CACGTG 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 2243
Bbv12I GWGCWC 3 cut(s) 3046, 3749, 4898
BceAI ACGGC 1 cut(s) 1119
BcgI CGANNNNNNTGC 4 cut(s) 945, 979, 2685, 2719
BciT130I CCWGG 4 cut(s) 3131, 3358, 4218, 4857
BciVI GTATCC 3 cut(s) 1773, 4295, 5086
BclI TGATCA 2 cut(s) 706, 1543
BcoDI GTCTC 3 cut(s) 262, 2118, 2640
BcuI ACTAGT 1 cut(s) 5021
BfaI CTAG 9 cut(s) 1028, 1370, 1439, 1736, 1766, 2244, 3222, 3801, 5022
BfmI CTRYAG 7 cut(s) 781, 1989, 2862, 3435, 4041, 4164, 4341
BfoI RGCGCY 1 cut(s) 5388
BfrI CTTAAG 2 cut(s) 1889, 3454
BfuAI ACCTGC 1 cut(s) 1108
BfuI GTATCC 3 cut(s) 1773, 4295, 5086
BglI GCCNNNNNGGC 2 cut(s) 518, 1187
BglII AGATCT 1 cut(s) 5317
BlpI GCTNAGC 3 cut(s) 584, 1716, 3577
BmcAI AGTACT 1 cut(s) 3368
Bme1390I CCNGG 4 cut(s) 3131, 3358, 4218, 4857
Bme18I GGWCC 9 cut(s) 409, 1270, 2819, 3071, 3323, 4148, 4415, 4485, 5312
BmeRI GACNNNNNGTC 1 cut(s) 433
BmeT110I CYCGRG 1 cut(s) 4698
BmgBI CACGTC 1 cut(s) 559
BmrFI CCNGG 4 cut(s) 3131, 3358, 4218, 4857
BpiI GAAGAC 1 cut(s) 2243
BplI GAGNNNNNCTC 4 cut(s) 2042, 2074, 4658, 4690
BpmI CTGGAG 4 cut(s) 1371, 2424, 2901, 5190
Bpu1102I GCTNAGC 3 cut(s) 584, 1716, 3577
Bpu14I TTCGAA 2 cut(s) 1701, 3599
BpuEI CTTGAG 5 cut(s) 251, 404, 513, 584, 3383
BsaAI YACGTR 3 cut(s) 328, 2579, 3338
BsaBI GATNNNNATC 2 cut(s) 83, 2889
BsaHI GRCGYC 4 cut(s) 255, 383, 429, 576
BsaWI WCCGGW 2 cut(s) 449, 4771
Bsc4I CCNNNNNNNGG 8 cut(s) 956, 1556, 2213, 2487, 4007, 4157, 4256, 5119
Bse118I RCCGGY 1 cut(s) 4834
Bse21I CCTNAGG 1 cut(s) 3726
Bse3DI GCAATG 8 cut(s) 745, 1470, 2104, 3296, 3525, 4785, 4952, 5065
Bse8I GATNNNNATC 2 cut(s) 83, 2889
BseBI CCWGG 4 cut(s) 3131, 3358, 4218, 4857
BseJI GATNNNNATC 2 cut(s) 83, 2889
BseLI CCNNNNNNNGG 8 cut(s) 956, 1556, 2213, 2487, 4007, 4157, 4256, 5119
BseMI GCAATG 8 cut(s) 745, 1470, 2104, 3296, 3525, 4785, 4952, 5065
BsePI GCGCGC 1 cut(s) 313
BseRI GAGGAG 6 cut(s) 1124, 1745, 2575, 4888, 4893, 4962
BseYI CCCAGC 2 cut(s) 3702, 5134
BsgI GTGCAG 6 cut(s) 340, 1214, 1404, 1952, 2327, 5566
Bsh1236I CGCG 4 cut(s) 315, 323, 336, 374
Bsh1285I CGRYCG 1 cut(s) 567
BshFI GGCC 9 cut(s) 353, 521, 2583, 3129, 3198, 3450, 3799, 3958, 4834
BshNI GGYRCC 2 cut(s) 4, 1559
BsiEI CGRYCG 1 cut(s) 567
BsiHKAI GWGCWC 3 cut(s) 3046, 3749, 4898
BsiHKCI CYCGRG 1 cut(s) 4698
BsiSI CCGG 8 cut(s) 401, 450, 540, 699, 1214, 3686, 4772, 4835
BslFI GGGAC 9 cut(s) 1283, 1507, 1878, 1885, 2296, 3427, 4498, 4602, 4799
BslI CCNNNNNNNGG 8 cut(s) 956, 1556, 2213, 2487, 4007, 4157, 4256, 5119
BsmAI GTCTC 3 cut(s) 262, 2118, 2640
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 9 cut(s) 1283, 1507, 1878, 1885, 2296, 3427, 4498, 4602, 4799
BsmI GAATGC 7 cut(s) 801, 1136, 2319, 2476, 4260, 5341, 5469
BsnI GGCC 9 cut(s) 353, 521, 2583, 3129, 3198, 3450, 3799, 3958, 4834
BsoBI CYCGRG 1 cut(s) 4698
Bsp119I TTCGAA 2 cut(s) 1701, 3599
Bsp1286I GDGCHC 4 cut(s) 2440, 3046, 3749, 4898
Bsp1407I TGTACA 1 cut(s) 3139
Bsp1720I GCTNAGC 3 cut(s) 584, 1716, 3577
BspANI GGCC 9 cut(s) 353, 521, 2583, 3129, 3198, 3450, 3799, 3958, 4834
BspFNI CGCG 4 cut(s) 315, 323, 336, 374
BspHI TCATGA 2 cut(s) 2323, 3493
BspMI ACCTGC 1 cut(s) 1108
BspPI GGATC 8 cut(s) 1403, 2988, 3659, 4367, 4380, 4497, 4510, 5156
BspQI GCTCTTC 6 cut(s) 303, 990, 1656, 2169, 2707, 3754
BspT104I TTCGAA 2 cut(s) 1701, 3599
BspT107I GGYRCC 2 cut(s) 4, 1559
BspTI CTTAAG 2 cut(s) 1889, 3454
BsrDI GCAATG 8 cut(s) 745, 1470, 2104, 3296, 3525, 4785, 4952, 5065
BsrFI RCCGGY 1 cut(s) 4834
BsrGI TGTACA 1 cut(s) 3139
BssAI RCCGGY 1 cut(s) 4834
BssHII GCGCGC 1 cut(s) 313
BssNI GRCGYC 4 cut(s) 255, 383, 429, 576
BssSI CACGAG 2 cut(s) 2616, 4761
BssT1I CCWWGG 7 cut(s) 285, 1058, 3378, 4006, 4581, 4829, 5172
Bst2BI CACGAG 2 cut(s) 2616, 4761
Bst2UI CCWGG 4 cut(s) 3131, 3358, 4218, 4857
BstACI GRCGYC 4 cut(s) 255, 383, 429, 576
BstAFI CTTAAG 2 cut(s) 1889, 3454
BstAPI GCANNNNNTGC 3 cut(s) 2072, 2369, 3307
BstAUI TGTACA 1 cut(s) 3139
BstBAI YACGTR 3 cut(s) 328, 2579, 3338
BstBI TTCGAA 2 cut(s) 1701, 3599
BstDSI CCRYGG 1 cut(s) 4488
BstENI CCTNNNNNAGG 1 cut(s) 954
BstFNI CGCG 4 cut(s) 315, 323, 336, 374
BstH2I RGCGCY 1 cut(s) 5388
BstMAI GTCTC 3 cut(s) 262, 2118, 2640
BstMCI CGRYCG 1 cut(s) 567
BstNI CCWGG 4 cut(s) 3131, 3358, 4218, 4857
BstNSI RCATGY 9 cut(s) 686, 1202, 1227, 1958, 2306, 2700, 3146, 3710, 3764
BstSCI CCNGG 4 cut(s) 3129, 3356, 4216, 4855
BstSFI CTRYAG 7 cut(s) 781, 1989, 2862, 3435, 4041, 4164, 4341
BstSNI TACGTA 1 cut(s) 3338
BstUI CGCG 4 cut(s) 315, 323, 336, 374
BstV2I GAAGAC 1 cut(s) 2243
BstX2I RGATCY 5 cut(s) 2980, 3664, 4372, 4502, 5317
BstXI CCANNNNNNTGG 1 cut(s) 4280
BstYI RGATCY 5 cut(s) 2980, 3664, 4372, 4502, 5317
Bsu36I CCTNAGG 1 cut(s) 3726
BsuI GTATCC 3 cut(s) 1773, 4295, 5086
BsuRI GGCC 9 cut(s) 353, 521, 2583, 3129, 3198, 3450, 3799, 3958, 4834
BtgI CCRYGG 1 cut(s) 4488
BtgZI GCGATG 1 cut(s) 717
BtrI CACGTC 1 cut(s) 559
BtsI GCAGTG 4 cut(s) 1398, 2370, 3087, 5060
BveI ACCTGC 1 cut(s) 1108
CaiI CAGNNNCTG 5 cut(s) 2096, 4868, 5141, 5458, 5591
CciI TCATGA 2 cut(s) 2323, 3493
Cfr10I RCCGGY 1 cut(s) 4834
CseI GACGC 6 cut(s) 363, 391, 437, 584, 2072, 3896
CsiI ACCWGGT 1 cut(s) 4216
Csp6I GTAC 9 cut(s) 944, 2681, 3140, 3339, 3367, 4011, 4997, 5007, 5087
CspCI CAANNNNNGTGG 2 cut(s) 1335, 1370
CviQI GTAC 9 cut(s) 944, 2681, 3140, 3339, 3367, 4011, 4997, 5007, 5087
DraI TTTAAA 1 cut(s) 240
DraIII CACNNNGTG 1 cut(s) 5573
DrdI GACNNNNNNGTC 1 cut(s) 563
DriI GACNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 563
EaeI YGGCCR 2 cut(s) 519, 4832
Eam1105I GACNNNNNGTC 1 cut(s) 433
EciI GGCGGA 2 cut(s) 378, 1121
Ecl136II GAGCTC 1 cut(s) 3747
Eco105I TACGTA 1 cut(s) 3338
Eco130I CCWWGG 7 cut(s) 285, 1058, 3378, 4006, 4581, 4829, 5172
Eco147I AGGCCT 2 cut(s) 3129, 3799
Eco24I GRGCYC 2 cut(s) 2440, 3749
Eco32I GATATC 2 cut(s) 2926, 3158
Eco47I GGWCC 9 cut(s) 409, 1270, 2819, 3071, 3323, 4148, 4415, 4485, 5312
Eco47III AGCGCT 1 cut(s) 5386
Eco53kI GAGCTC 1 cut(s) 3747
Eco72I CACGTG 1 cut(s) 328
Eco81I CCTNAGG 1 cut(s) 3726
Eco88I CYCGRG 1 cut(s) 4698
EcoICRI GAGCTC 1 cut(s) 3747
EcoNI CCTNNNNNAGG 1 cut(s) 954
EcoO109I RGGNCCY 1 cut(s) 1270
EcoRI GAATTC 3 cut(s) 1338, 2453, 5123
EcoRII CCWGG 4 cut(s) 3129, 3356, 4216, 4855
EcoRV GATATC 2 cut(s) 2926, 3158
EcoT14I CCWWGG 7 cut(s) 285, 1058, 3378, 4006, 4581, 4829, 5172
EcoT22I ATGCAT 2 cut(s) 2241, 3433
EcoT38I GRGCYC 2 cut(s) 2440, 3749
ErhI CCWWGG 7 cut(s) 285, 1058, 3378, 4006, 4581, 4829, 5172
Esp3I CGTCTC 1 cut(s) 262
FalI AAGNNNNNCTT 6 cut(s) 717, 749, 1695, 1727, 2148, 2180
FaqI GGGAC 9 cut(s) 1283, 1507, 1878, 1885, 2296, 3427, 4498, 4602, 4799
FauI CCCGC 3 cut(s) 3802, 3834, 4798
FauNDI CATATG 3 cut(s) 2779, 3817, 5491
FbaI TGATCA 2 cut(s) 706, 1543
FblI GTMKAC 5 cut(s) 567, 1161, 3885, 4073, 5485
FriOI GRGCYC 2 cut(s) 2440, 3749
FspBI CTAG 9 cut(s) 1028, 1370, 1439, 1736, 1766, 2244, 3222, 3801, 5022
FspI TGCGCA 1 cut(s) 462
GsaI CCCAGC 2 cut(s) 3706, 5138
GsuI CTGGAG 4 cut(s) 1371, 2424, 2901, 5190
HaeII RGCGCY 1 cut(s) 5388
HaeIII GGCC 9 cut(s) 353, 521, 2583, 3129, 3198, 3450, 3799, 3958, 4834
HapII CCGG 8 cut(s) 401, 450, 540, 699, 1214, 3686, 4772, 4835
HgaI GACGC 6 cut(s) 363, 391, 437, 584, 2072, 3896
Hin1I GRCGYC 4 cut(s) 255, 383, 429, 576
HincII GTYRAC 6 cut(s) 178, 568, 1162, 1300, 4985, 5486
HindII GTYRAC 6 cut(s) 178, 568, 1162, 1300, 4985, 5486
HindIII AAGCTT 5 cut(s) 731, 762, 1002, 1718, 3317
HpaI GTTAAC 1 cut(s) 1300
HpaII CCGG 8 cut(s) 401, 450, 540, 699, 1214, 3686, 4772, 4835
Hpy99I CGWCG 7 cut(s) 142, 158, 364, 385, 431, 824, 1166
Hsp92I GRCGYC 4 cut(s) 255, 383, 429, 576
Ksp22I TGATCA 2 cut(s) 706, 1543
KspAI GTTAAC 1 cut(s) 1300
LguI GCTCTTC 6 cut(s) 303, 990, 1656, 2169, 2707, 3754
MabI ACCWGGT 1 cut(s) 4216
MaeI CTAG 9 cut(s) 1028, 1370, 1439, 1736, 1766, 2244, 3222, 3801, 5022
MfeI CAATTG 2 cut(s) 3240, 5289
MflI RGATCY 5 cut(s) 2980, 3664, 4372, 4502, 5317
MhlI GDGCHC 4 cut(s) 2440, 3046, 3749, 4898
MlyI GAGTC 9 cut(s) 563, 2642, 2738, 3383, 3409, 4062, 4584, 5186, 5449
MmeI TCCRAC 2 cut(s) 4659, 5356
Mph1103I ATGCAT 2 cut(s) 2241, 3433
MroXI GAANNNNTTC 5 cut(s) 1136, 1697, 3681, 3720, 5414
MslI CAYNNNNRTG 9 cut(s) 89, 687, 1959, 2606, 3107, 4278, 4394, 5053, 5426
MspA1I CMGCKG 6 cut(s) 1232, 1418, 3218, 3809, 3838, 4691
MspCI CTTAAG 2 cut(s) 1889, 3454
MspI CCGG 8 cut(s) 401, 450, 540, 699, 1214, 3686, 4772, 4835
MspR9I CCNGG 4 cut(s) 3131, 3358, 4218, 4857
MunI CAATTG 2 cut(s) 3240, 5289
Mva1269I GAATGC 7 cut(s) 801, 1136, 2319, 2476, 4260, 5341, 5469
MvaI CCWGG 4 cut(s) 3131, 3358, 4218, 4857
MvnI CGCG 4 cut(s) 315, 323, 336, 374
NdeI CATATG 3 cut(s) 2779, 3817, 5491
NmeAIII GCCGAG 2 cut(s) 497, 604
NmuCI GTSAC 8 cut(s) 375, 554, 670, 809, 1429, 1466, 5017, 5354
NsbI TGCGCA 1 cut(s) 462
NsiI ATGCAT 2 cut(s) 2241, 3433
NspI RCATGY 9 cut(s) 686, 1202, 1227, 1958, 2306, 2700, 3146, 3710, 3764
NspV TTCGAA 2 cut(s) 1701, 3599
OliI CACNNNNGTG 1 cut(s) 4394
PaeI GCATGC 2 cut(s) 1958, 2700
PagI TCATGA 2 cut(s) 2323, 3493
PasI CCCWGGG 1 cut(s) 4856
PauI GCGCGC 1 cut(s) 313
PceI AGGCCT 2 cut(s) 3129, 3799
PciI ACATGT 5 cut(s) 682, 1223, 2302, 3142, 3760
PciSI GCTCTTC 6 cut(s) 303, 990, 1656, 2169, 2707, 3754
PcsI WCGNNNNNNNCGW 2 cut(s) 333, 564
PctI GAATGC 7 cut(s) 801, 1136, 2319, 2476, 4260, 5341, 5469
PdmI GAANNNNTTC 5 cut(s) 1136, 1697, 3681, 3720, 5414
PflMI CCANNNNNTGG 2 cut(s) 2213, 5119
PleI GAGTC 9 cut(s) 563, 2641, 2737, 3382, 3409, 4061, 4584, 5185, 5449
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
PpsI GAGTC 9 cut(s) 563, 2641, 2737, 3382, 3409, 4061, 4584, 5185, 5449
Ppu21I YACGTR 3 cut(s) 328, 2579, 3338
PpuMI RGGWCCY 1 cut(s) 1270
PscI ACATGT 5 cut(s) 682, 1223, 2302, 3142, 3760
Psp124BI GAGCTC 1 cut(s) 3749
Psp1406I AACGTT 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 1270
Psp6I CCWGG 4 cut(s) 3129, 3356, 4216, 4855
PspCI CACGTG 1 cut(s) 328
PspFI CCCAGC 2 cut(s) 3702, 5134
PspGI CCWGG 4 cut(s) 3129, 3356, 4216, 4855
PspPPI RGGWCCY 1 cut(s) 1270
PstNI CAGNNNCTG 5 cut(s) 2096, 4868, 5141, 5458, 5591
PsuI RGATCY 5 cut(s) 2980, 3664, 4372, 4502, 5317
PteI GCGCGC 1 cut(s) 313
PvuII CAGCTG 2 cut(s) 1232, 4691
RsaI GTAC 9 cut(s) 945, 2682, 3141, 3340, 3368, 4012, 4998, 5008, 5088
RsaNI GTAC 9 cut(s) 944, 2681, 3140, 3339, 3367, 4011, 4997, 5007, 5087
RseI CAYNNNNRTG 9 cut(s) 89, 687, 1959, 2606, 3107, 4278, 4394, 5053, 5426
SacI GAGCTC 1 cut(s) 3749
SalI GTCGAC 3 cut(s) 566, 1160, 5484
SapI GCTCTTC 6 cut(s) 303, 990, 1656, 2169, 2707, 3754
ScaI AGTACT 1 cut(s) 3368
SchI GAGTC 9 cut(s) 563, 2642, 2738, 3383, 3409, 4062, 4584, 5186, 5449
ScrFI CCNGG 4 cut(s) 3131, 3358, 4218, 4857
SduI GDGCHC 4 cut(s) 2440, 3046, 3749, 4898
SexAI ACCWGGT 1 cut(s) 4216
SfcI CTRYAG 7 cut(s) 781, 1989, 2862, 3435, 4041, 4164, 4341
SfuI TTCGAA 2 cut(s) 1701, 3599
SinI GGWCC 9 cut(s) 409, 1270, 2819, 3071, 3323, 4148, 4415, 4485, 5312
SmiMI CAYNNNNRTG 9 cut(s) 89, 687, 1959, 2606, 3107, 4278, 4394, 5053, 5426
SmlI CTYRAG 7 cut(s) 266, 419, 528, 599, 1889, 3362, 3454
SmoI CTYRAG 7 cut(s) 266, 419, 528, 599, 1889, 3362, 3454
SnaBI TACGTA 1 cut(s) 3338
SpeI ACTAGT 1 cut(s) 5021
SphI GCATGC 2 cut(s) 1958, 2700
SseBI AGGCCT 2 cut(s) 3129, 3799
SspI AATATT 2 cut(s) 1924, 4849
SspMI CTAG 9 cut(s) 1028, 1370, 1439, 1736, 1766, 2244, 3222, 3801, 5022
SstI GAGCTC 1 cut(s) 3749
StuI AGGCCT 2 cut(s) 3129, 3799
StyD4I CCNGG 4 cut(s) 3129, 3356, 4216, 4855
StyI CCWWGG 7 cut(s) 285, 1058, 3378, 4006, 4581, 4829, 5172
TatI WGTACW 3 cut(s) 2680, 3139, 3366
TauI GCSGC 7 cut(s) 334, 394, 515, 3221, 3518, 3826, 3841
TseFI GTSAC 8 cut(s) 375, 554, 670, 809, 1429, 1466, 5017, 5354
Tsp45I GTSAC 8 cut(s) 375, 554, 670, 809, 1429, 1466, 5017, 5354
TspGWI ACGGA 3 cut(s) 155, 1509, 4039
Van91I CCANNNNNTGG 2 cut(s) 2213, 5119
Vha464I CTTAAG 2 cut(s) 1889, 3454
VpaK11BI GGWCC 9 cut(s) 409, 1270, 2819, 3071, 3323, 4148, 4415, 4485, 5312
XagI CCTNNNNNAGG 1 cut(s) 954
XapI RAATTY 7 cut(s) 1338, 2453, 3303, 3733, 4016, 4468, 5123
XceI RCATGY 9 cut(s) 686, 1202, 1227, 1958, 2306, 2700, 3146, 3710, 3764
XmiI GTMKAC 5 cut(s) 567, 1161, 3885, 4073, 5485
XmnI GAANNNNTTC 5 cut(s) 1136, 1697, 3681, 3720, 5414
XspI CTAG 9 cut(s) 1028, 1370, 1439, 1736, 1766, 2244, 3222, 3801, 5022
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 1 cut(s) 3368
Zsp2I ATGCAT 2 cut(s) 2241, 3433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.