RLG00000027870

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0007431
Physical Location & Seq
Reverse (-)
16262620 .. 16273433
10814 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027870

Sequence Viewer

Length: 5604 bp
ATGGCACCCAGAAACAGTCGTGGAAAGGGCAAAGGTGGAGAGAAGAAGAAGAAGGAAGAGAAGGTTCTTCCTGTTGTGATGGACATCACTGTGAACCTTCCAGATGAAAGCGGTGTTGTTTTGAAGGGAATATCGACGGACAGGATCATCGACGTTCGCCAACTATTATCAGTCAACACAGAAACTTGTAACATCACCAATTTTTCACTATCCCATGAGGTGAGAGGGAAACGTTTAAAAGATACGGTGGACGTCTCCGCACTCAAGCCATGCGTCATCACCTTGGTCGAAGAGGACTATGATGAAGAGCGCGCCACCGCGCACGTGCGGCGCGTGCTGGACATCGTGGCATGCACGACGAGCTTTGGCGAGTCACCGACGTCGCCGCCGCCGACTCCGGCGAAGGACCAAAGCCTCAAGCCCGACGCCTCGTCCGCAGTCCTCTCCGGTAAAAATGCGCAGGATAATAAGACCGCCAAGAAATCCACCGCCACCGCCACCGCCACCGCCACCGCCGCCGCGGCCAAGCCTCAAGGCTCTGCCGGAGCCGATAAGCGTGACATGGCGGTCGACTCGGACGCCGAGCTGAGCCACTCTTGCCTCAAGCTCGGTTCTTTCTACGACTTCTTCTCTCTCGCTCATCTCACTCCTCCTCTGCAATTTATCCGAAGGGTGACGAAACGACATGTCGATGAGATTTCCGGCGATGATCACCTGTTTTCTCTTGAAGTGAAGCTTTGCAATGGGAAGGTGGTTCTGGTAGAAGCTTGCAGAAAGGGATTCTATAGTGTAGGGAAGCAGCGCATTCTGTGTCACAACCTGGTCGATTTGCTACGCCAGCTTAGTAGAGCTTTTGACAATGCTTACGATGATCTGCTGAAAGCATTTTCGGAACGTAATAAGTTTGGGAATCTTCCCTATGGCTTTCGAGCAAACACATGGCTTGTACCGCCTATTGCAGGGCAGTCGCCTTCAGGTTTCCCTGCTCTTCCTGTGGAGGATGAAGCTTGGGGAGGCAATGGAGGGGGTCTAGGAAGGGATGGGCAATTTGATCTGATTCCTTGGGCTAATGAGTTTTGGTATATTGCATCTATGCCTTGCAGGACGGCGGAGGAGAGGCAGGTTCGAGATAGGAAGGCATTCCTTCTTCACAGCCTATTTGTCGACGTTTCCATTTTTAGAGCCATTAAGGCTGTGCAGCATGTGATGGCAAAGCCGGAATTAACATGTTCAGCTGCAAACAGTGAGGTTCTTTATACTGAGAGAGTAGGGGACCTGAATGTGATAGTAACAAAGGATGTTAACAATGCGAGCTGTAAGGTAGATACTAAAATTGATGGAATTCAGGCAACTGGAGTGGATAAAAAGAATCTAGCACAACGAAACCTTCTTAAAGGGATTACTGCTGACGAGAACACTGCTGCACATGATGTCACCACTCTAGGTGTTGTCAATGTCCGATATTGTGGTCACATTGCAGTTGTGAAAGTTGAGGGCAAAGAAACTAAGAAAGTCAGTTCTCCATCCCAAAGCATTGACCTCCTTGATCAACCTGAAGGTGGTGCCAATGCCCTTAATATCAACAGTTTGAGATTACTACTTCACACTACTACACCTTCCGAGCAGAATAAACTGGCATCACATGGGCAAGGTCCGGAACATGAAGAGCTTTCTGTTTCCTGCGCTTTTGTTGAGAATCTGTTAGAAGAAAGTCTTGCTAAGCTTGACAAAGAGGAGCTAGATTCTGATAGTTTTGTGAGATGGGAACTAGGAGCTTGCTGGATACAACACTTGCAAGACCAAAAAAATACAGACAAGGATAAGAAACCATTGGGTGAGAAGGCTAAGAATGAGATGAAGGTCGAGGGACTTGGGACACCTCTTAAATCCCTTAAGAACACGAAGAAGAAATCAGATGGAGCCAATATTAAAGTGCAGTCTGATAGCTCAAAGTCACATGCAGATGGTGTTGTGGGAGAAGTGGAAAATGCTATAGGTTCTGTGGAATCAAAGTTTGAAACCAGTGCTAAAGAAAATGAGCTTGTGCTGACAGAGATGCTCTCTGACGCAGCCTTTGCTCGACTAAAAGAATCAGAAACTGGACTTCATTGCAAGTCTCTGCAAGAACTGATCGACTTATCTCAGAAGTATTACTCTGAAGTTGCTCTTCCAAAACTGGTAGCCGATTTTGGTTCATTGGAACTTTCACCAGTTGATGGTCGAACTTTAACTGATTTCATGCATACTAGAGGTCTTCGCATGCGTTCTCTGGGGCAAGTTGTCAAGCTATCAGAGAAGCTATCACATGTGCAGTCCCTTTGCATTCATGAGATGATAGTGCGAGCTTTTAAGCACATACTTCAGGCAGTGATTGCTGCTGTTAATAACACTGAGAAAATGGCTGCCTCGATTGCTGCTGCATTGAATCTTATGTTGGGGGCTCCAGAAAATGAAGAATTCAATAAGTCTTTCAATGTGCATTCCCTTGTGTGGAGATGGCTGGAAGTATTCTTGCGAAAGAGATATGGATGGGATGTTAGCAGCTTCAACTACAATGATGTGAGGAGATTTGCGATTCTACGTGGCCTGTGTCATAAGGTGGGCATAGAGATGGTTCCACGAGATTTTGATATGGAGTCTCCGAATCCATTTCAGAGTTCTGATATTGTCAGCTTAGTTCCAGTACATAAGCAAGCTGCATGCTCTTCGGCAGATGGTAGGCAACTGTTGGAGTCATCAAAAACAGCCTTAGATAAGGGAAAACTTGAAGATGCAGTTGCATATGGCACTAAGGCTCTTGCAAAGCTGGTAGCAGTTTGTGGTCCATACCATCGAATGACAGCAGGAGCTTACAGCCTCCTTGCTGTAGTTTTATATCACACTGGAGATTTTAATCAGGCTACAATTTATCAGCAAAAGGCTCTGGATATCAATGAGAGAGAATTAGGGCTTGACCATCCAGACACCATGAAGAGTTATGGGGATCTTGCTGTGTTCTATTACAGACTTCAACATACAGAGCTGGCTCTCAAATATGTAAAACGTGCTCTGTATCTCTTGCATCTCACATGCGGACCATCTCATCCAAACACTGCTGCAACATACATCAACGTGGCTATGATGGAAGAAGGCCTGGGAAATGTACATGTTGCCCTCAGATATCTACACAAGGCTTTGAAGTGTAATCAGAAGTTACTTGGCCCAGATCATATTCAGACAGCGGCTAGTTACCATGCTATTGCAATTGCACTCTCATTGATGGAAGCATACCCTTTAAGTGTTCAGCATGAGCAGACAACATTGCAAATTCTGCGTGCAAAGCTTGGACCAGATGATCTACGTACACAAGATGCTGCTGCCTGGCTTGAGTACTTTGAGTCCAAGGCTTTTGAACAGCAAGAAGCTGCAAGAAATGGGACTCGAAAACCAGATGCATCTATAGCCAGCAAAGGCCACTTAAGCGTATCAGATTTGCTTGACTACATCAATCCCACTCATGATGCTAAAGGGAGAGATGCGGCAATGAAGAGGAAAGCCTACATAACAAAGTTGAAGGAAAAATCCTATCAAACTATCAGCTCAGCAAGTTCTGACGAATCTTCAAAAGAAACCACAAAAGAGGTTTCAGACGAAGAAACACATGCAATTGAACCAAGAGAAAGAACAGATCCCATCCAAGAAAGCATCCCGGCTTCAGTTGAACCCCAGCATGTTGTAGAAGAGATTCCTGAGGAAAATTCAAACATGAGCTCTTCTGAAACACATGTTGAAGGGGAAGATGACTGGCAACCAGTTCAAAGGCCTAGATCAGCGGGTTCATATGGGCGGCGTGTAAAGCACCGGCGGGCAGCTATTGGGAAGGTTTATAGTTACCAGAAGAAATATGTAGACGCTGATATGGATTACCCTTCAGTGAAGAATACTAACCAAAACAGTAGGTATTACCTTCTAAAGAAAAGGCCAACATCTCATGGAAGTTATACAGAAAATCAGATTGCGAATCCCTCCCAAGGTACTAAATTTGGACGGAGAATGGTAAAAGCTGTAGCATACAGAGTCAAGTCTATGCCTTTGTCTACCAAAGTTGTTGCAGCAGAAAGTTTGAGTTCCTCATCAGAAGTCAGTCCAAATGTTTCACCACATGGGATTGGTCCAGTTAAAGGTTCTATAGTCAGTCTTGGAAAATCTCCTTCATACAAGGAAGTAGCATTGGCTCCACCAGGTAGTATTGCTAAAATGCAAACTGTTTTCCCTCAGAATGGCATTCCAGATAACCATGAGCATGGTATCCAAAGACAAGAAGAGGAAATAATTGAAATCAAAGGAGATTCCAAACCAAAGGCTGTAGTTGAAGCTATACTTGAGGAAAAGAAGGATCCTGTATTGGTTTCCACAGATGTGTTACGAGAGCAAACTGGACCTGTTGAGAAGAAAGAAGATAATAATTCACCTGAAGTGAAGGAAGATAAAAATTCACTGATATTCGGGACCGTGGATGAACTTGGATCCAGTGCTGTTAAGTTTCAAGAGGCAGCAGTTGATAACGTATTAGTAGATGGACTGCCAAAATCCATTGATTCTGCCAAGGAGGGACTCAGTGAGAACAATCCATCTGGCACTTCTGAATTGCTTGATTCCAACTCCACTTTGCAAGGAGTAGATGATTTAGAGGCATCCTCGCTTGTTACTTCAGCTGATACTCGGGGATTGCCAAATAAGAAGTTATCTGCATCGGCAGCTCCATTCAATCCCTCACCATCCACTCGTTCTGCTCCAGTTCCCATGAACATTGCTATACCTTCTGGTGCGGGTTCTGTCCCAACTATTGCACCTTGGCCAGTAAACCTGAATATTCACCCAGGGGCAGCTACTGTCTTACCCACAGTTAATCCCATGTGCTCCTCTCCTCATCATCCATACCCTTCTCCCCCTGCTACCCCGAATATCATACAGCCATTGCCATTTATGTTCCCTCCTCCTTACAGTCAACCCCAAGTTGTACGAACCAGTACCTTTCCTGTGACTAGTGCGTTTCATCCAAATCATTACACATGGCAGTGCAATGTGAATCCTAATGTATCTGAGTTTGTACCTACAACAGTTTGGCATGGTTGCCATCCAATGGAATTCTCTGCCCCAGTACCTGTTGTTGAGCCAATTTCTGATCCCTCTCCCTTGGAGTCAAAAATTCAGAACGATGATTCTGCTCCAGTTCTGCCAGTGGACATTGATAATGTGGGGGAAACTAAGAAAGAAGCGGACATTCTAACATCAGAGGCAACTTGTAATCCAATTGAAAGTGTCAAGGCAGTTGGTCCAGATCTTTGTGGGGTTGAGAATGCTCAGAACGAACCATGTGACAGCCCAAATGGAAGTTTTGGAAGTAGCGCTGAAAGGACGACTGATGGAGAGAAAACCTTCAGCATTTTGATAAGGGGCAGAAGAAACCGAAAGCAGACTCTGAGAATGCCAGTAAGTTTGCTCAGTCGACCATATGGCTCACAGTCATTCAAAGTCATATATAACAGGGTGGTCAGGGGTAATAATGCGTCCAAACCCATCAGCTTTTCATCTAGTGAAAACTTCACAGCTACTGCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1868

Amino Acids

204.22

Weight (kDa)

6.3

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 46 - 116 9.3e-11 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 735 - 877 2.5e-24 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 917 - 979 2.3e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 919 - 1017 1.8e-07 Nephrocystin-3 TPR domain
TPR_10 PF13374 947 - 988 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 947 - 1017 1.5e-12 Tetratricopeptide repeat
TPR_10 PF13374 1031 - 1071 3.4e-07 Tetratricopeptide repeat
TPR_12 PF13424 1031 - 1105 5.3e-12 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 566
AatII GACGTC 2 cut(s) 255, 383
Acc16I TGCGCA 1 cut(s) 459
Acc36I ACCTGC 1 cut(s) 1111
AccB1I GGYRCC 2 cut(s) 4, 1562
AccB7I CCANNNNNTGG 2 cut(s) 2216, 5122
AccI GTMKAC 5 cut(s) 570, 1164, 3888, 4076, 5488
AccII CGCG 4 cut(s) 312, 320, 333, 521
AccIII TCCGGA 1 cut(s) 1654
AclI AACGTT 1 cut(s) 232
AclWI GGATC 8 cut(s) 152, 2991, 3662, 4370, 4383, 4500, 4513, 5159
AcoI YGGCCR 2 cut(s) 522, 4835
AcsI RAATTY 8 cut(s) 1341, 2456, 3306, 3736, 4019, 4471, 5126, 5187
AcuI CTGAAG 9 cut(s) 957, 1575, 2178, 2345, 3678, 3894, 4473, 4674, 5404
AcvI CACGTG 1 cut(s) 325
AcyI GRCGYC 4 cut(s) 252, 380, 426, 579
AfeI AGCGCT 1 cut(s) 5389
AfiI CCNNNNNNNGG 8 cut(s) 959, 1559, 2216, 2490, 4010, 4160, 4259, 5122
AflII CTTAAG 2 cut(s) 1892, 3457
AflIII ACRYGT 5 cut(s) 685, 1226, 2305, 3145, 3763
AhdI GACNNNNNGTC 1 cut(s) 430
AhlI ACTAGT 1 cut(s) 5024
AjnI CCWGG 5 cut(s) 819, 3132, 3359, 4219, 4858
AjuI GAANNNNNNNTTGG 2 cut(s) 2417, 2449
AleI CACNNNNGTG 1 cut(s) 4397
Alw21I GWGCWC 3 cut(s) 3049, 3752, 4901
Alw26I GTCTC 3 cut(s) 259, 2121, 2643
AlwI GGATC 8 cut(s) 152, 2991, 3662, 4370, 4383, 4500, 4513, 5159
AlwNI CAGNNNCTG 5 cut(s) 2099, 4871, 5144, 5461, 5594
Ama87I CYCGRG 1 cut(s) 4701
Aor13HI TCCGGA 1 cut(s) 1654
Aor51HI AGCGCT 1 cut(s) 5389
AoxI GGCC 8 cut(s) 522, 2584, 3130, 3199, 3451, 3800, 3959, 4835
ApoI RAATTY 8 cut(s) 1341, 2456, 3306, 3736, 4019, 4471, 5126, 5187
ArsI GACNNNNNNTTYG 2 cut(s) 2057, 2089
Asp700I GAANNNNTTC 3 cut(s) 1139, 3723, 5417
AspLEI GCGC 8 cut(s) 312, 314, 322, 333, 460, 804, 1685, 5390
AsuC2I CCSGG 1 cut(s) 3689
AvaI CYCGRG 1 cut(s) 4701
AxyI CCTNAGG 1 cut(s) 3729
BalI TGGCCA 1 cut(s) 4837
BamHI GGATCC 2 cut(s) 4375, 4505
BanI GGYRCC 2 cut(s) 4, 1562
BanII GRGCYC 2 cut(s) 2443, 3752
BauI CACGAG 1 cut(s) 2619
BbrPI CACGTG 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 2246
Bbv12I GWGCWC 3 cut(s) 3049, 3752, 4901
BceAI ACGGC 1 cut(s) 1122
BcgI CGANNNNNNTGC 8 cut(s) 948, 982, 1400, 1434, 2688, 2722, 5186, 5220
BciT130I CCWGG 5 cut(s) 821, 3134, 3361, 4221, 4860
BciVI GTATCC 2 cut(s) 1776, 4298
BclI TGATCA 2 cut(s) 709, 1546
BcnI CCSGG 1 cut(s) 3689
BcoDI GTCTC 3 cut(s) 259, 2121, 2643
BcuI ACTAGT 1 cut(s) 5024
BfmI CTRYAG 7 cut(s) 784, 1992, 2865, 3438, 4044, 4167, 4344
BfoI RGCGCY 1 cut(s) 5391
BfrI CTTAAG 2 cut(s) 1892, 3457
BfuAI ACCTGC 1 cut(s) 1111
BfuI GTATCC 2 cut(s) 1776, 4298
BglI GCCNNNNNGGC 2 cut(s) 521, 1190
BglII AGATCT 1 cut(s) 5320
BlpI GCTNAGC 3 cut(s) 587, 1719, 3580
BmcAI AGTACT 1 cut(s) 3371
Bme1390I CCNGG 6 cut(s) 821, 3134, 3361, 3689, 4221, 4860
BmeRI GACNNNNNGTC 1 cut(s) 430
BmeT110I CYCGRG 1 cut(s) 4701
BmrFI CCNGG 6 cut(s) 821, 3134, 3361, 3689, 4221, 4860
BmrI ACTGGG 1 cut(s) 5132
BmuI ACTGGG 1 cut(s) 5132
BpiI GAAGAC 1 cut(s) 2246
BplI GAGNNNNNCTC 4 cut(s) 2045, 2077, 4661, 4693
BpmI CTGGAG 5 cut(s) 1374, 2427, 2904, 4758, 5193
Bpu1102I GCTNAGC 3 cut(s) 587, 1719, 3580
BpuEI CTTGAG 6 cut(s) 248, 401, 516, 587, 3386, 4382
BpuMI CCSGG 1 cut(s) 3689
BsaAI YACGTR 3 cut(s) 325, 2582, 3341
BsaBI GATNNNNATC 2 cut(s) 83, 2892
BsaHI GRCGYC 4 cut(s) 252, 380, 426, 579
BsaWI WCCGGW 2 cut(s) 446, 1654
Bsc4I CCNNNNNNNGG 8 cut(s) 959, 1559, 2216, 2490, 4010, 4160, 4259, 5122
Bse118I RCCGGY 1 cut(s) 3840
Bse21I CCTNAGG 1 cut(s) 3729
Bse3DI GCAATG 9 cut(s) 748, 1024, 1473, 2107, 3299, 3528, 4788, 4955, 5068
Bse8I GATNNNNATC 2 cut(s) 83, 2892
BseAI TCCGGA 1 cut(s) 1654
BseBI CCWGG 5 cut(s) 821, 3134, 3361, 4221, 4860
BseJI GATNNNNATC 2 cut(s) 83, 2892
BseLI CCNNNNNNNGG 8 cut(s) 959, 1559, 2216, 2490, 4010, 4160, 4259, 5122
BseMI GCAATG 9 cut(s) 748, 1024, 1473, 2107, 3299, 3528, 4788, 4955, 5068
BsePI GCGCGC 1 cut(s) 310
BseRI GAGGAG 8 cut(s) 639, 642, 1127, 1748, 2578, 4891, 4896, 4965
BseYI CCCAGC 1 cut(s) 3705
BsgI GTGCAG 4 cut(s) 1217, 1407, 1955, 2330
Bsh1236I CGCG 4 cut(s) 312, 320, 333, 521
Bsh1285I CGRYCG 1 cut(s) 570
BshFI GGCC 8 cut(s) 524, 2586, 3132, 3201, 3453, 3802, 3961, 4837
BshNI GGYRCC 2 cut(s) 4, 1562
BsiEI CGRYCG 1 cut(s) 570
BsiHKAI GWGCWC 3 cut(s) 3049, 3752, 4901
BsiHKCI CYCGRG 1 cut(s) 4701
BsiSI CCGG 8 cut(s) 398, 447, 543, 702, 1217, 1655, 3689, 3841
BslFI GGGAC 8 cut(s) 1286, 1881, 1888, 2299, 3430, 4501, 4605, 4802
BslI CCNNNNNNNGG 8 cut(s) 959, 1559, 2216, 2490, 4010, 4160, 4259, 5122
BsmAI GTCTC 3 cut(s) 259, 2121, 2643
BsmBI CGTCTC 1 cut(s) 259
BsmFI GGGAC 8 cut(s) 1286, 1881, 1888, 2299, 3430, 4501, 4605, 4802
BsmI GAATGC 7 cut(s) 804, 1139, 2322, 2479, 4263, 5344, 5472
BsnI GGCC 8 cut(s) 524, 2586, 3132, 3201, 3453, 3802, 3961, 4837
BsoBI CYCGRG 1 cut(s) 4701
Bsp1286I GDGCHC 4 cut(s) 2443, 3049, 3752, 4901
Bsp13I TCCGGA 1 cut(s) 1654
Bsp1407I TGTACA 1 cut(s) 3142
Bsp1720I GCTNAGC 3 cut(s) 587, 1719, 3580
BspANI GGCC 8 cut(s) 524, 2586, 3132, 3201, 3453, 3802, 3961, 4837
BspEI TCCGGA 1 cut(s) 1654
BspFNI CGCG 4 cut(s) 312, 320, 333, 521
BspHI TCATGA 2 cut(s) 2326, 3496
BspMI ACCTGC 1 cut(s) 1111
BspPI GGATC 8 cut(s) 152, 2991, 3662, 4370, 4383, 4500, 4513, 5159
BspQI GCTCTTC 6 cut(s) 300, 993, 1659, 2172, 2710, 3757
BspT107I GGYRCC 2 cut(s) 4, 1562
BspTI CTTAAG 2 cut(s) 1892, 3457
BsrDI GCAATG 9 cut(s) 748, 1024, 1473, 2107, 3299, 3528, 4788, 4955, 5068
BsrFI RCCGGY 1 cut(s) 3840
BsrGI TGTACA 1 cut(s) 3142
BssAI RCCGGY 1 cut(s) 3840
BssHII GCGCGC 1 cut(s) 310
BssNI GRCGYC 4 cut(s) 252, 380, 426, 579
BssSI CACGAG 1 cut(s) 2619
BssT1I CCWWGG 7 cut(s) 282, 1061, 3381, 4009, 4584, 4832, 5175
Bst2BI CACGAG 1 cut(s) 2619
Bst2UI CCWGG 5 cut(s) 821, 3134, 3361, 4221, 4860
BstACI GRCGYC 4 cut(s) 252, 380, 426, 579
BstAFI CTTAAG 2 cut(s) 1892, 3457
BstAPI GCANNNNNTGC 3 cut(s) 2075, 2372, 3310
BstAUI TGTACA 1 cut(s) 3142
BstBAI YACGTR 3 cut(s) 325, 2582, 3341
BstDSI CCRYGG 2 cut(s) 519, 4491
BstENI CCTNNNNNAGG 1 cut(s) 957
BstFNI CGCG 4 cut(s) 312, 320, 333, 521
BstH2I RGCGCY 1 cut(s) 5391
BstHHI GCGC 8 cut(s) 312, 314, 322, 333, 460, 804, 1685, 5390
BstMAI GTCTC 3 cut(s) 259, 2121, 2643
BstMCI CGRYCG 1 cut(s) 570
BstNI CCWGG 5 cut(s) 821, 3134, 3361, 4221, 4860
BstSCI CCNGG 6 cut(s) 819, 3132, 3359, 3687, 4219, 4858
BstSFI CTRYAG 7 cut(s) 784, 1992, 2865, 3438, 4044, 4167, 4344
BstSNI TACGTA 1 cut(s) 3341
BstUI CGCG 4 cut(s) 312, 320, 333, 521
BstV2I GAAGAC 1 cut(s) 2246
BstX2I RGATCY 5 cut(s) 2983, 3667, 4375, 4505, 5320
BstXI CCANNNNNNTGG 1 cut(s) 4283
BstYI RGATCY 5 cut(s) 2983, 3667, 4375, 4505, 5320
Bsu36I CCTNAGG 1 cut(s) 3729
BsuI GTATCC 2 cut(s) 1776, 4298
BsuRI GGCC 8 cut(s) 524, 2586, 3132, 3201, 3453, 3802, 3961, 4837
BtgI CCRYGG 2 cut(s) 519, 4491
BtgZI GCGATG 1 cut(s) 720
BtsI GCAGTG 4 cut(s) 1416, 2373, 3090, 5063
BveI ACCTGC 1 cut(s) 1111
CaiI CAGNNNCTG 5 cut(s) 2099, 4871, 5144, 5461, 5594
CciI TCATGA 2 cut(s) 2326, 3496
CfoI GCGC 8 cut(s) 312, 314, 322, 333, 460, 804, 1685, 5390
Cfr10I RCCGGY 1 cut(s) 3840
Cfr42I CCGCGG 1 cut(s) 522
CseI GACGC 6 cut(s) 262, 434, 587, 2075, 3899, 5538
CsiI ACCWGGT 2 cut(s) 819, 4219
CspCI CAANNNNNGTGG 2 cut(s) 1338, 1373
DraI TTTAAA 1 cut(s) 237
DrdI GACNNNNNNGTC 1 cut(s) 566
DriI GACNNNNNGTC 1 cut(s) 430
DseDI GACNNNNNNGTC 1 cut(s) 566
EaeI YGGCCR 2 cut(s) 522, 4835
Eam1105I GACNNNNNGTC 1 cut(s) 430
EciI GGCGGA 1 cut(s) 1124
Ecl136II GAGCTC 1 cut(s) 3750
Eco105I TACGTA 1 cut(s) 3341
Eco130I CCWWGG 7 cut(s) 282, 1061, 3381, 4009, 4584, 4832, 5175
Eco147I AGGCCT 2 cut(s) 3132, 3802
Eco24I GRGCYC 2 cut(s) 2443, 3752
Eco32I GATATC 2 cut(s) 2929, 3161
Eco47III AGCGCT 1 cut(s) 5389
Eco53kI GAGCTC 1 cut(s) 3750
Eco57I CTGAAG 9 cut(s) 957, 1575, 2178, 2345, 3678, 3894, 4473, 4674, 5404
Eco72I CACGTG 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 3729
Eco88I CYCGRG 1 cut(s) 4701
EcoICRI GAGCTC 1 cut(s) 3750
EcoNI CCTNNNNNAGG 1 cut(s) 957
EcoO109I RGGNCCY 1 cut(s) 1273
EcoRI GAATTC 3 cut(s) 1341, 2456, 5126
EcoRII CCWGG 5 cut(s) 819, 3132, 3359, 4219, 4858
EcoRV GATATC 2 cut(s) 2929, 3161
EcoT14I CCWWGG 7 cut(s) 282, 1061, 3381, 4009, 4584, 4832, 5175
EcoT22I ATGCAT 2 cut(s) 2244, 3436
EcoT38I GRGCYC 2 cut(s) 2443, 3752
ErhI CCWWGG 7 cut(s) 282, 1061, 3381, 4009, 4584, 4832, 5175
Esp3I CGTCTC 1 cut(s) 259
FalI AAGNNNNNCTT 8 cut(s) 720, 752, 1698, 1730, 2151, 2183, 4344, 4376
FaqI GGGAC 8 cut(s) 1286, 1881, 1888, 2299, 3430, 4501, 4605, 4802
FauI CCCGC 3 cut(s) 3805, 3837, 4801
FauNDI CATATG 3 cut(s) 2782, 3820, 5494
FbaI TGATCA 2 cut(s) 709, 1546
FblI GTMKAC 5 cut(s) 570, 1164, 3888, 4076, 5488
FriOI GRGCYC 2 cut(s) 2443, 3752
FspI TGCGCA 1 cut(s) 459
GlaI GCGC 8 cut(s) 311, 313, 321, 332, 459, 803, 1684, 5389
GsaI CCCAGC 1 cut(s) 3709
GsuI CTGGAG 5 cut(s) 1374, 2427, 2904, 4758, 5193
HaeII RGCGCY 1 cut(s) 5391
HaeIII GGCC 8 cut(s) 524, 2586, 3132, 3201, 3453, 3802, 3961, 4837
HapII CCGG 8 cut(s) 398, 447, 543, 702, 1217, 1655, 3689, 3841
HgaI GACGC 6 cut(s) 262, 434, 587, 2075, 3899, 5538
HhaI GCGC 8 cut(s) 312, 314, 322, 333, 460, 804, 1685, 5390
Hin1I GRCGYC 4 cut(s) 252, 380, 426, 579
Hin6I GCGC 8 cut(s) 310, 312, 320, 331, 458, 802, 1683, 5388
HinP1I GCGC 8 cut(s) 310, 312, 320, 331, 458, 802, 1683, 5388
HincII GTYRAC 6 cut(s) 175, 571, 1165, 1303, 4988, 5489
HindII GTYRAC 6 cut(s) 175, 571, 1165, 1303, 4988, 5489
HindIII AAGCTT 5 cut(s) 734, 765, 1005, 1721, 3320
HpaI GTTAAC 1 cut(s) 1303
HpaII CCGG 8 cut(s) 398, 447, 543, 702, 1217, 1655, 3689, 3841
Hpy99I CGWCG 7 cut(s) 139, 155, 361, 382, 385, 428, 1169
Hsp92I GRCGYC 4 cut(s) 252, 380, 426, 579
HspAI GCGC 8 cut(s) 310, 312, 320, 331, 458, 802, 1683, 5388
Kpn2I TCCGGA 1 cut(s) 1654
Ksp22I TGATCA 2 cut(s) 709, 1546
KspAI GTTAAC 1 cut(s) 1303
KspI CCGCGG 1 cut(s) 522
LguI GCTCTTC 6 cut(s) 300, 993, 1659, 2172, 2710, 3757
MabI ACCWGGT 2 cut(s) 819, 4219
MfeI CAATTG 3 cut(s) 3243, 3645, 5292
MflI RGATCY 5 cut(s) 2983, 3667, 4375, 4505, 5320
MhlI GDGCHC 4 cut(s) 2443, 3049, 3752, 4901
MlsI TGGCCA 1 cut(s) 4837
MluNI TGGCCA 1 cut(s) 4837
MmeI TCCRAC 2 cut(s) 2709, 4662
Mox20I TGGCCA 1 cut(s) 4837
Mph1103I ATGCAT 2 cut(s) 2244, 3436
MroI TCCGGA 1 cut(s) 1654
MroXI GAANNNNTTC 3 cut(s) 1139, 3723, 5417
MscI TGGCCA 1 cut(s) 4837
MslI CAYNNNNRTG 8 cut(s) 89, 690, 1962, 2609, 3110, 4281, 4397, 5056
Msp20I TGGCCA 1 cut(s) 4837
MspA1I CMGCKG 5 cut(s) 521, 1235, 3221, 3812, 4694
MspCI CTTAAG 2 cut(s) 1892, 3457
MspI CCGG 8 cut(s) 398, 447, 543, 702, 1217, 1655, 3689, 3841
MspR9I CCNGG 6 cut(s) 821, 3134, 3361, 3689, 4221, 4860
MunI CAATTG 3 cut(s) 3243, 3645, 5292
Mva1269I GAATGC 7 cut(s) 804, 1139, 2322, 2479, 4263, 5344, 5472
MvaI CCWGG 5 cut(s) 821, 3134, 3361, 4221, 4860
MvnI CGCG 4 cut(s) 312, 320, 333, 521
NciI CCSGG 1 cut(s) 3689
NdeI CATATG 3 cut(s) 2782, 3820, 5494
NmeAIII GCCGAG 1 cut(s) 607
NmuCI GTSAC 9 cut(s) 372, 557, 673, 812, 1432, 1469, 1953, 5020, 5357
NsbI TGCGCA 1 cut(s) 459
NsiI ATGCAT 2 cut(s) 2244, 3436
OliI CACNNNNGTG 1 cut(s) 4397
PaeI GCATGC 3 cut(s) 354, 2263, 2703
PagI TCATGA 2 cut(s) 2326, 3496
PasI CCCWGGG 1 cut(s) 4859
PauI GCGCGC 1 cut(s) 310
PceI AGGCCT 2 cut(s) 3132, 3802
PciI ACATGT 5 cut(s) 685, 1226, 2305, 3145, 3763
PciSI GCTCTTC 6 cut(s) 300, 993, 1659, 2172, 2710, 3757
PcsI WCGNNNNNNNCGW 2 cut(s) 330, 389
PctI GAATGC 7 cut(s) 804, 1139, 2322, 2479, 4263, 5344, 5472
PdmI GAANNNNTTC 3 cut(s) 1139, 3723, 5417
PflMI CCANNNNNTGG 2 cut(s) 2216, 5122
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
Ppu21I YACGTR 3 cut(s) 325, 2582, 3341
PpuMI RGGWCCY 1 cut(s) 1273
PscI ACATGT 5 cut(s) 685, 1226, 2305, 3145, 3763
Psp124BI GAGCTC 1 cut(s) 3752
Psp1406I AACGTT 1 cut(s) 232
Psp5II RGGWCCY 1 cut(s) 1273
Psp6I CCWGG 5 cut(s) 819, 3132, 3359, 4219, 4858
PspCI CACGTG 1 cut(s) 325
PspFI CCCAGC 1 cut(s) 3705
PspGI CCWGG 5 cut(s) 819, 3132, 3359, 4219, 4858
PspPPI RGGWCCY 1 cut(s) 1273
PstNI CAGNNNCTG 5 cut(s) 2099, 4871, 5144, 5461, 5594
PsuI RGATCY 5 cut(s) 2983, 3667, 4375, 4505, 5320
PteI GCGCGC 1 cut(s) 310
PvuII CAGCTG 2 cut(s) 1235, 4694
RseI CAYNNNNRTG 8 cut(s) 89, 690, 1962, 2609, 3110, 4281, 4397, 5056
SacI GAGCTC 1 cut(s) 3752
SacII CCGCGG 1 cut(s) 522
SalI GTCGAC 3 cut(s) 569, 1163, 5487
SapI GCTCTTC 6 cut(s) 300, 993, 1659, 2172, 2710, 3757
ScaI AGTACT 1 cut(s) 3371
ScrFI CCNGG 6 cut(s) 821, 3134, 3361, 3689, 4221, 4860
SduI GDGCHC 4 cut(s) 2443, 3049, 3752, 4901
SexAI ACCWGGT 2 cut(s) 819, 4219
SfcI CTRYAG 7 cut(s) 784, 1992, 2865, 3438, 4044, 4167, 4344
Sfr303I CCGCGG 1 cut(s) 522
SgrAI CRCCGGYG 1 cut(s) 3840
SgrBI CCGCGG 1 cut(s) 522
SmiMI CAYNNNNRTG 8 cut(s) 89, 690, 1962, 2609, 3110, 4281, 4397, 5056
SmlI CTYRAG 8 cut(s) 263, 416, 531, 602, 1892, 3365, 3457, 4361
SmoI CTYRAG 8 cut(s) 263, 416, 531, 602, 1892, 3365, 3457, 4361
SnaBI TACGTA 1 cut(s) 3341
SpeI ACTAGT 1 cut(s) 5024
SphI GCATGC 3 cut(s) 354, 2263, 2703
SseBI AGGCCT 2 cut(s) 3132, 3802
SspI AATATT 2 cut(s) 1927, 4852
SstI GAGCTC 1 cut(s) 3752
StuI AGGCCT 2 cut(s) 3132, 3802
StyD4I CCNGG 6 cut(s) 819, 3132, 3359, 3687, 4219, 4858
StyI CCWWGG 7 cut(s) 282, 1061, 3381, 4009, 4584, 4832, 5175
TatI WGTACW 3 cut(s) 2683, 3142, 3369
TauI GCSGC 9 cut(s) 331, 388, 391, 518, 521, 524, 3224, 3521, 3829
TseFI GTSAC 9 cut(s) 372, 557, 673, 812, 1432, 1469, 1953, 5020, 5357
Tsp45I GTSAC 9 cut(s) 372, 557, 673, 812, 1432, 1469, 1953, 5020, 5357
TspGWI ACGGA 2 cut(s) 152, 4042
Van91I CCANNNNNTGG 2 cut(s) 2216, 5122
Vha464I CTTAAG 2 cut(s) 1892, 3457
XagI CCTNNNNNAGG 1 cut(s) 957
XapI RAATTY 8 cut(s) 1341, 2456, 3306, 3736, 4019, 4471, 5126, 5187
XmiI GTMKAC 5 cut(s) 570, 1164, 3888, 4076, 5488
XmnI GAANNNNTTC 3 cut(s) 1139, 3723, 5417
ZraI GACGTC 2 cut(s) 253, 381
ZrmI AGTACT 1 cut(s) 3371
Zsp2I ATGCAT 2 cut(s) 2244, 3436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.