Rw1G025280

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007431
Physical Location & Seq
Forward (+)
51846359 .. 51858495
12137 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025280.1

Sequence Viewer

Length: 5484 bp
ATGGCACCCAGAAACAGTCGTGGAAAGGGCAAAGGTGGAGAGAAGAAGAAGAAGGAAGAGAAGGTTCTTCCTGTTGTGATGGACATCACTGTGAACCTTCCAGATGAAAGCAGTGTTGTTTTGAAGGGAATATCGACGGACAGGATCATCGACGTTCGCCAACTATTATCAGTCAACACAGAAACTTGTAACATCACCAATTTTTCACTATTCCATGAGGTAAGAGGGAAACGTTTAAAAGATACGGTGGACGTCTCCGCACTCAAGCCATGCGTCATGACCTTGGTCGAAGAGGACTATGATGAAGAGCGCTCCACCGCGCACGTGCGGCGCGTGCTGGACATCGTGGCCTGCACGACGAGCTTTGGCGCGTCACCGACGCCTCCACCGACTCCGGCGAAGGACCAAAGCCTCAAGCCCGACGCCTCGTCCGCAGTCCTCTCCGGTAAAAATGCGCAGGATAATAAGACCGCCAAGAAATCCACCGCCACCTCCACCGCGGCGGCGGCCAAGTCTCAAGGCTCTGCCGGAGCCGATAAGTGTGACGTGGCGGTCGACTCGGACGCCGAGCTGAGCCACTCTTGCCTCAAGCTCGGTTCTTTCTACGACTTCTTCTCTCTCGCTCATCTCACTCCCCCGCTGCAATTTATCCGAAGGGTGACGAAACGACATGTCGATGAGATTTCCGGCGATGATCACCTGTTTTCTCTTGAAGTGAAGCTTTGCAATGGGAAGGTGGTTCTGGTTGAAGCTTGCAGAAAGGGATTCTATAGTGTAGGGAAGCAGCGCATTCTGTGTCACAACCTCGTCGATTTGCTACGCCAGCTTAGTAGAGCTTTTGACAATGCTTACGATGATCTGCTGAAAGCATTTTCGGAACGTAATAAGTTTGGGAATCTTCCCTATGGCTTTCGAGCAAACACATGGCTTGTACCGCCTATTGCAGGGCAGTCGCCTTCAGGTTTCCCTGCTCTTCCTGTGGAGGATGAAGCTTGGGGAGGAAATGGAGGGGGTCTAGGAAGGGATGGGCAATTTGATCTGATTCCTTGGGCTAATGAGTTTTGGTGTATTGCATCTATGCCTTGCAAGACGGCGGAGGAGAGGCAGGTTCGAGATAGGAAGGCATTCCTTCTTCACAGCCTATTTGTCGACGTTTCCATTTTTAGAGCCATTAAGGCTGTGCAGCATGTGATGGCAAAGCCGGAATTAACATGTTCAGCTGCAAACAGTGAGGTTCTTTATACTGAGAGAGTAGGGGACTTGAATGTGATAGTAACAAAGGATGTTAACAATGCGAGCTGTAAGGTGGATACTAAAATTGATGGAATTCAGGCAACTGGAGTGGATAAAAAGAATCTAGCACAACGAAACCTTCTTAAAGGGATCACTGCTGACGAGAACACCGCTGCACATGATGTCACCACTCTTGGTGTTGTCAATGTCCGATATTGTGGTCACATTGCAGTTGTGAAAGTTGAGGGCAAAGAAACTAAGAAAGTCAGTTCTCCATCCCAAAGCATTGACCTCCTTGATCAACCTGAAGGTGGTGCCAATGCCCTTAATATCAACAGTTTGAGATTACTACTTCACACTACTACACCTTCAGAGCAGAATAAACTGGCATCACATGGGCAAGGTCTGGAACATGAAGTGCTTTCTGCTTCCTGCGCTTTTGTTGAGAAGCTATTAGAAGAAAGTCTTGCTAAGCTTGACAAAGAGGAGCTAGATTCTGATAGTTTTGTGAGATGGGAACTAGGAGCTTGCTGGATACAACACTTGCAAGACCAAAAAAATACAGACAAGGATAAGAAACCATTGGGTGAGAAGGCTAAGAATGAGATGAAGGTCGAGGGACTTGGGACACCTCTTAAATCCCTTAAGAACACGAAGAAGAAATCAGATGGAGCCAATATTAAAGTGCAGTCTGATAGCTCAAAGTCGCATGCAGATGGTGTTGTGGGAGAAGTAGAAAATGCTATAGGTTCTGTGGAATCAAAGTTTGAAACCAGTGCTAAAGAAAATGAGCTTGTGCTGACAGAGATGCTCTCTGACGCAGCCTTTGCTCGACTAAAAGAATCAGAAACTGGACTTCATTGCAAGTCTCTGCAAGAACTGATCGACTTATCTCAGAAGTATTACTCTGAAGTTGCTCTTCCAAAACTGGTAGCCGATTTTGGTTCATTGGAACTTTCACCAGTTGATGGTCGAACTTTAACTGATTTCATGCATACTAGAGGTCTTCGGATGCGTTCTCTGGGGCAAGTTGTCAAGCTATCAGAGAAGCTATCACATGTGCAGTCCCTTTGTATTCATGAGATGATAGTGCGAGCTTTTAAGCATATACTTCAGGCAGTGATTGCTGCTGTTAATGACACTGAGAAAATGGCTGCCTCGATTGCTGCTGCATTGAATCTTATGTTGGGGGCTCCAGAAAATGAAGAATTCAATAAGTCTTTCAATGTGCATTCCCTTGTGTGGAGATGGCTGGAATTATTCTTGCGAAAGAGATATGGATGGGATGTTAGCAGCTTCAACTACAATGATGTGAGGAGATTTGCGATTCTACGTGGCCTGTGTCATAAGGTGGGCATTGAGATGGTTCCACGAGATTTTGATATGGAGTCTCCAAATCCATTTAAGAGTTCTGATATTGTCAGCTTAGTTCCAGTACATAAGCAAGCTGCATGCTCTTCGGCAGATGGTAGGCAACTATTGGAGTCATCAAAAACAGCCTTAGACAAGGGAAAACTTGAAGATGCAGTTGCATATGGCACTAAGGCTACAATTTATCAGCAAAAGTCTCTGGATATCAATGAGAGAGAATTAGGGCTTGACCATCCAGACACCATGAAGAGTTATGGGGATCTTGCTGTGTTCTATTACAGACTTCAACATACAGAGCTGGCTCTCAAATATGTAAAACGTGCTCTGTATCTCTTGCATCTCACATGTGGACCATCTCATCCAAACACTGCTGCAACATACATCAACGTGGCTATGATGGAAGAAGGCCTGGGAAATGTACATGTTGCCCTCAGATATCTACACAAGGCTTTGAAGTGTAATCAGAAGTTACTTGGCCCAGATCATATTCAGACAGCGGCTAGTTACCATGCTATTGCAATTGCACTCTCATTGATGGAAGCATACCCTTTAAGTGTTCAGCATGAGCAGACAACATTGCAAATTCTGCGTGCAAAGCTTGGACCAGATGATCTACGTACACAAGATGCTGCTGCCTGGCTTGAGTACTTTGAGTCCAAGGCTTTTGAACAGCAAGAAGCTGCAAGAAATGGGACTCGAAAACCAGATGCATCTATAGCCAGCAAAGGCCACTTAAGCGTATCAGATTTGCTTGACTACATCAATCCCACTGATGATGCTAAAGGGAGAGATGCGGCAATGAAGAGGAAAACCTACATAACAAAGTTGAAGGAAAAATCCTATCAAACTATCAGCTCAGCAAGTTCTGACGAATCTTCAAAAGAAACCACAAAAGAGGTTTCAGATGAAGAAACACATACACTTGAACCAAGAGAAAGAACAGATCCCATCCAAGAAAACATCCCGGCTTCAGTTGAACCCCAGCATGTTGTAGAAGAGATTCCTGAGGAAAATTCAAACATGAGCTCTTCTGAAACACATGTTGAAGGGGAAGATGACTGGCAACCAGTTCAAAGGCCTAGATCAGCGGGTTCATATGGGCGGCGTGTAAAACAGCGGCGGGCAGCTATTGGGAAGGTTTATAGTTACCAGAAGAAATATGTAGATGCTGATATGGATTACCCTTCAGTGAAGAATACTAACCAAAACAGTAGGTATTACCTGCTAAAGAAAAGGCCAACATCTCATGGAAGTTATACAGAAAATCAGATTGCGAATCCCTCCCAAGGTACTAAATTTGGACGGAGAATGGTAAAAGCTGTAGCATACAGAGTCAAGTCTATGCCTTTGTCTACCAAAGTCGTTGCAGCAGAAAGTTTGAGTTCCTCATCAGAAGTCAGTCCAAATGTTTCACCACATGGGATTGGTCCAGTTAAAGGTTCTATAGTCAGTCTTGGAAAATCTCCTTCATACAAGGAAGTAGCATTGGCTCCACCAGGTAGTATTGCTAAAATGCAAACTGTTTTCCCTCAGAATGGCATTCCAGATAACCATGAGCATGGTATCCAAAGACAAGAAGAGGAAATAATTGAAATCAAAGGAGATTCCAAACCAAAGGCTGTAGTTGAAACTATACTTGAAGAAAAGAAGGATCCTGTATTGGTTTCCACAGATGTGTTACGACAGCAAACTGGACCTGTTGAGAAGAAAGAAGATACTAATTCACCTGAAGTGAAGGAAGATAAAAATTCACTGATATTCGGGACCGTGGATGAACTTGGATCCAGTGCTGTTAAGTTTCAAGCGGCAGCAGTTGATAACGTATTAGTAGATGGACTGCCAAAATCCATTGATTCTGCCAAGGAGGGACTCTGTGAGAACAATCCATCTGGCACTTCTGAATTGCATGATTCCAACTCCACTTTGCAAGGAGTAGATGATTTGGAGGCATCCTTGCTTGTTACTTCAGCTGATACTCGGGGATTGCCAAATAAGAAGTTATCTGCATCGGCAGCTCCATTCAATCCCTCACCATCCACTCGTGCTGCTCCGGTTCCCATGAACATTGCTATACCTTCTGGTGCGGGTTCTGTCCCAACTATTGCACCTTGGCCAGTAAACCTGAATATTCACCCAGGGGCAGCTACTGTCTTACCCACAGTTAATCCCATGTGCTCCTCTCCTCATCATCCATACCCTTCTCCCCCTGCTACTCCGAATATCATACAGCCATTGCCATTTATGTTCCCTCCTCCTTACAGTCAACCCCAAGTTGTACGAACCAGTACCTTTCCTGTGACTAGTGCGTTTCATCCAAATCATTACACATGGCAGTGCAATGTGAATCCTAATGTATCCGAGTTTGTACCTACAACAGTTTGGCATGGTTGCCATCCAATGGAATTCTCTGCCCCAGCACCTGTTGTTGAGCCAATTTCTGATCCCTCTCCCTTGGAGTCAAAAATTCAGAACGATGATTCTGCTCCAGTTCTGCCAGTGGACATTGGTAATGTGGGGGAAACTAAGAAAGAAGCGGACATTCTAACATCAGAGGCAACTTGCAATCCAATTGAAAGTGTCAAGGAAGTTGGTCCAGATCTTTGTGGGGTTGAGAATGCTCAGAACGAACCATGTGACAGCCCAAATGGAAAAGTTGGAAGTAGCGCTGAAAGGATGACTGATGGAGAGAAAACCTTCAGCATTTTGATAAGGGGCAGAAGAAACCGAAAGCAGACTCTGAGAATGCCAGTAAGTTTGCTCAGTCGACCATATGGCTCACAGTCATTCAAAGTCATATATAACAGGGTGGTCAGGGGGAATGATGCGTCCAAACCCATCAGCTTTTCATCTAGTGAAAACTGCACAGCTACTGCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1827

Amino Acids

199.98

Weight (kDa)

6.33

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 46 - 116 1.6e-10 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 730 - 872 2.7e-24 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 926 - 977 2e-06 Tetratricopeptide repeat
TPR_10 PF13374 991 - 1031 3.3e-07 Tetratricopeptide repeat
TPR_12 PF13424 991 - 1065 5.2e-12 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 551
AatII GACGTC 1 cut(s) 255
Acc16I TGCGCA 1 cut(s) 456
Acc36I ACCTGC 2 cut(s) 1096, 3834
AccB1I GGYRCC 2 cut(s) 4, 1547
AccB7I CCANNNNNTGG 2 cut(s) 2201, 5002
AccI GTMKAC 4 cut(s) 555, 1149, 3956, 5368
AccII CGCG 4 cut(s) 320, 333, 371, 500
AclI AACGTT 1 cut(s) 232
AclWI GGATC 9 cut(s) 152, 1391, 2871, 3542, 4250, 4263, 4380, 4393, 5039
AcoI YGGCCR 2 cut(s) 507, 4715
AcsI RAATTY 8 cut(s) 1326, 2441, 3186, 3616, 3899, 4351, 5006, 5067
AcvI CACGTG 1 cut(s) 325
AcyI GRCGYC 4 cut(s) 252, 380, 423, 564
AfaI GTAC 9 cut(s) 933, 2670, 3024, 3223, 3251, 3895, 4881, 4891, 4971
AfeI AGCGCT 2 cut(s) 311, 5269
AfiI CCNNNNNNNGG 8 cut(s) 944, 1544, 2201, 2475, 3890, 4040, 4139, 5002
AflII CTTAAG 2 cut(s) 1877, 3337
AflIII ACRYGT 6 cut(s) 670, 1211, 2290, 2948, 3025, 3643
AhdI GACNNNNNGTC 1 cut(s) 427
AhlI ACTAGT 1 cut(s) 4904
AjiI CACGTC 1 cut(s) 547
AjnI CCWGG 4 cut(s) 3012, 3239, 4099, 4738
AjuI GAANNNNNNNTTGG 2 cut(s) 2402, 2434
AleI CACNNNNGTG 1 cut(s) 4277
Alw21I GWGCWC 3 cut(s) 2929, 3632, 4781
Alw26I GTCTC 5 cut(s) 259, 519, 2106, 2628, 2805
AlwI GGATC 9 cut(s) 152, 1391, 2871, 3542, 4250, 4263, 4380, 4393, 5039
AlwNI CAGNNNCTG 5 cut(s) 2084, 4751, 5024, 5341, 5474
Ama87I CYCGRG 1 cut(s) 4581
Aor51HI AGCGCT 2 cut(s) 311, 5269
AoxI GGCC 9 cut(s) 348, 507, 2569, 3010, 3079, 3331, 3680, 3839, 4715
ApoI RAATTY 8 cut(s) 1326, 2441, 3186, 3616, 3899, 4351, 5006, 5067
ArsI GACNNNNNNTTYG 2 cut(s) 2042, 2074
Asp700I GAANNNNTTC 3 cut(s) 1124, 3603, 5297
AspLEI GCGC 8 cut(s) 312, 322, 333, 371, 457, 789, 1670, 5270
AspS9I GGNCC 8 cut(s) 403, 2954, 3080, 3206, 4031, 4298, 4368, 5195
AsuC2I CCSGG 1 cut(s) 3569
AvaI CYCGRG 1 cut(s) 4581
AvaII GGWCC 7 cut(s) 403, 2954, 3206, 4031, 4298, 4368, 5195
AxyI CCTNAGG 1 cut(s) 3609
BalI TGGCCA 1 cut(s) 4717
BamHI GGATCC 2 cut(s) 4255, 4385
BanI GGYRCC 2 cut(s) 4, 1547
BanII GRGCYC 2 cut(s) 2428, 3632
BauI CACGAG 2 cut(s) 2604, 4644
BbrPI CACGTG 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 2231
Bbv12I GWGCWC 3 cut(s) 2929, 3632, 4781
BceAI ACGGC 1 cut(s) 1107
BcgI CGANNNNNNTGC 6 cut(s) 933, 967, 2673, 2707, 5066, 5100
BciT130I CCWGG 4 cut(s) 3014, 3241, 4101, 4740
BciVI GTATCC 4 cut(s) 1303, 1761, 4178, 4969
BclI TGATCA 2 cut(s) 694, 1531
BcnI CCSGG 1 cut(s) 3569
BcoDI GTCTC 5 cut(s) 259, 519, 2106, 2628, 2805
BcuI ACTAGT 1 cut(s) 4904
BfaI CTAG 9 cut(s) 1016, 1358, 1724, 1754, 2232, 3105, 3684, 4905, 5454
BfmI CTRYAG 6 cut(s) 769, 1977, 3318, 3924, 4047, 4224
BfoI RGCGCY 2 cut(s) 313, 5271
BfrI CTTAAG 2 cut(s) 1877, 3337
BfuAI ACCTGC 2 cut(s) 1096, 3834
BfuI GTATCC 4 cut(s) 1303, 1761, 4178, 4969
BglI GCCNNNNNGGC 1 cut(s) 1175
BglII AGATCT 1 cut(s) 5200
BlpI GCTNAGC 3 cut(s) 572, 1704, 3460
BmcAI AGTACT 1 cut(s) 3251
Bme1390I CCNGG 5 cut(s) 3014, 3241, 3569, 4101, 4740
Bme18I GGWCC 7 cut(s) 403, 2954, 3206, 4031, 4298, 4368, 5195
BmeRI GACNNNNNGTC 1 cut(s) 427
BmeT110I CYCGRG 1 cut(s) 4581
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 8 cut(s) 403, 2954, 3080, 3206, 4031, 4298, 4368, 5195
BmrFI CCNGG 5 cut(s) 3014, 3241, 3569, 4101, 4740
BoxI GACNNNNGTC 1 cut(s) 284
BpiI GAAGAC 1 cut(s) 2231
BplI GAGNNNNNCTC 2 cut(s) 2030, 2062
BpmI CTGGAG 3 cut(s) 1359, 2412, 5073
Bpu1102I GCTNAGC 3 cut(s) 572, 1704, 3460
BpuEI CTTGAG 5 cut(s) 248, 398, 501, 572, 3266
BpuMI CCSGG 1 cut(s) 3569
BsaAI YACGTR 3 cut(s) 325, 2567, 3221
BsaBI GATNNNNATC 1 cut(s) 83
BsaHI GRCGYC 4 cut(s) 252, 380, 423, 564
BsaWI WCCGGW 2 cut(s) 443, 4654
Bsc4I CCNNNNNNNGG 8 cut(s) 944, 1544, 2201, 2475, 3890, 4040, 4139, 5002
Bse21I CCTNAGG 1 cut(s) 3609
Bse3DI GCAATG 8 cut(s) 733, 1458, 2092, 3179, 3408, 4668, 4835, 4948
Bse8I GATNNNNATC 1 cut(s) 83
BseBI CCWGG 4 cut(s) 3014, 3241, 4101, 4740
BseJI GATNNNNATC 1 cut(s) 83
BseLI CCNNNNNNNGG 8 cut(s) 944, 1544, 2201, 2475, 3890, 4040, 4139, 5002
BseMI GCAATG 8 cut(s) 733, 1458, 2092, 3179, 3408, 4668, 4835, 4948
BseRI GAGGAG 6 cut(s) 1112, 1733, 2563, 4771, 4776, 4845
BseYI CCCAGC 2 cut(s) 3585, 5017
BsgI GTGCAG 6 cut(s) 337, 1202, 1392, 1940, 2315, 5449
Bsh1236I CGCG 4 cut(s) 320, 333, 371, 500
Bsh1285I CGRYCG 1 cut(s) 555
BshFI GGCC 9 cut(s) 350, 509, 2571, 3012, 3081, 3333, 3682, 3841, 4717
BshNI GGYRCC 2 cut(s) 4, 1547
BsiEI CGRYCG 1 cut(s) 555
BsiHKAI GWGCWC 3 cut(s) 2929, 3632, 4781
BsiHKCI CYCGRG 1 cut(s) 4581
BsiSI CCGG 7 cut(s) 395, 444, 528, 687, 1202, 3569, 4655
BslFI GGGAC 8 cut(s) 1271, 1866, 1873, 2284, 3310, 4381, 4485, 4682
BslI CCNNNNNNNGG 8 cut(s) 944, 1544, 2201, 2475, 3890, 4040, 4139, 5002
BsmAI GTCTC 5 cut(s) 259, 519, 2106, 2628, 2805
BsmBI CGTCTC 1 cut(s) 259
BsmFI GGGAC 8 cut(s) 1271, 1866, 1873, 2284, 3310, 4381, 4485, 4682
BsmI GAATGC 6 cut(s) 789, 1124, 2464, 4143, 5224, 5352
BsnI GGCC 9 cut(s) 350, 509, 2571, 3012, 3081, 3333, 3682, 3841, 4717
BsoBI CYCGRG 1 cut(s) 4581
Bsp1286I GDGCHC 4 cut(s) 2428, 2929, 3632, 4781
Bsp1407I TGTACA 1 cut(s) 3022
Bsp1720I GCTNAGC 3 cut(s) 572, 1704, 3460
BspANI GGCC 9 cut(s) 350, 509, 2571, 3012, 3081, 3333, 3682, 3841, 4717
BspFNI CGCG 4 cut(s) 320, 333, 371, 500
BspHI TCATGA 2 cut(s) 276, 2311
BspMI ACCTGC 2 cut(s) 1096, 3834
BspPI GGATC 9 cut(s) 152, 1391, 2871, 3542, 4250, 4263, 4380, 4393, 5039
BspQI GCTCTTC 5 cut(s) 300, 978, 2157, 2695, 3637
BspT107I GGYRCC 2 cut(s) 4, 1547
BspTI CTTAAG 2 cut(s) 1877, 3337
BsrDI GCAATG 8 cut(s) 733, 1458, 2092, 3179, 3408, 4668, 4835, 4948
BsrGI TGTACA 1 cut(s) 3022
BssNI GRCGYC 4 cut(s) 252, 380, 423, 564
BssSI CACGAG 2 cut(s) 2604, 4644
BssT1I CCWWGG 7 cut(s) 282, 1046, 3261, 3889, 4464, 4712, 5055
Bst2BI CACGAG 2 cut(s) 2604, 4644
Bst2UI CCWGG 4 cut(s) 3014, 3241, 4101, 4740
BstACI GRCGYC 4 cut(s) 252, 380, 423, 564
BstAFI CTTAAG 2 cut(s) 1877, 3337
BstAPI GCANNNNNTGC 3 cut(s) 2060, 2357, 3190
BstAUI TGTACA 1 cut(s) 3022
BstBAI YACGTR 3 cut(s) 325, 2567, 3221
BstDSI CCRYGG 2 cut(s) 498, 4371
BstENI CCTNNNNNAGG 1 cut(s) 942
BstFNI CGCG 4 cut(s) 320, 333, 371, 500
BstH2I RGCGCY 2 cut(s) 313, 5271
BstHHI GCGC 8 cut(s) 312, 322, 333, 371, 457, 789, 1670, 5270
BstMAI GTCTC 5 cut(s) 259, 519, 2106, 2628, 2805
BstMCI CGRYCG 1 cut(s) 555
BstNI CCWGG 4 cut(s) 3014, 3241, 4101, 4740
BstPAI GACNNNNGTC 1 cut(s) 284
BstSCI CCNGG 5 cut(s) 3012, 3239, 3567, 4099, 4738
BstSFI CTRYAG 6 cut(s) 769, 1977, 3318, 3924, 4047, 4224
BstSNI TACGTA 1 cut(s) 3221
BstUI CGCG 4 cut(s) 320, 333, 371, 500
BstV2I GAAGAC 1 cut(s) 2231
BstX2I RGATCY 5 cut(s) 2863, 3547, 4255, 4385, 5200
BstXI CCANNNNNNTGG 1 cut(s) 4163
BstYI RGATCY 5 cut(s) 2863, 3547, 4255, 4385, 5200
Bsu36I CCTNAGG 1 cut(s) 3609
BsuI GTATCC 4 cut(s) 1303, 1761, 4178, 4969
BsuRI GGCC 9 cut(s) 350, 509, 2571, 3012, 3081, 3333, 3682, 3841, 4717
BtgI CCRYGG 2 cut(s) 498, 4371
BtgZI GCGATG 1 cut(s) 705
BtrI CACGTC 1 cut(s) 547
BtsI GCAGTG 5 cut(s) 118, 1386, 2358, 2970, 4943
BveI ACCTGC 2 cut(s) 1096, 3834
CaiI CAGNNNCTG 5 cut(s) 2084, 4751, 5024, 5341, 5474
CciI TCATGA 2 cut(s) 276, 2311
CfoI GCGC 8 cut(s) 312, 322, 333, 371, 457, 789, 1670, 5270
Cfr13I GGNCC 8 cut(s) 403, 2954, 3080, 3206, 4031, 4298, 4368, 5195
Cfr42I CCGCGG 1 cut(s) 501
CseI GACGC 7 cut(s) 262, 360, 388, 431, 572, 2060, 5418
CsiI ACCWGGT 1 cut(s) 4099
Csp6I GTAC 9 cut(s) 932, 2669, 3023, 3222, 3250, 3894, 4880, 4890, 4970
CspCI CAANNNNNGTGG 2 cut(s) 1323, 1358
CviQI GTAC 9 cut(s) 932, 2669, 3023, 3222, 3250, 3894, 4880, 4890, 4970
DraI TTTAAA 1 cut(s) 237
DrdI GACNNNNNNGTC 1 cut(s) 551
DriI GACNNNNNGTC 1 cut(s) 427
DseDI GACNNNNNNGTC 1 cut(s) 551
EaeI YGGCCR 2 cut(s) 507, 4715
Eam1105I GACNNNNNGTC 1 cut(s) 427
EciI GGCGGA 1 cut(s) 1109
Ecl136II GAGCTC 1 cut(s) 3630
Eco105I TACGTA 1 cut(s) 3221
Eco130I CCWWGG 7 cut(s) 282, 1046, 3261, 3889, 4464, 4712, 5055
Eco147I AGGCCT 2 cut(s) 3012, 3682
Eco24I GRGCYC 2 cut(s) 2428, 3632
Eco32I GATATC 2 cut(s) 2809, 3041
Eco47I GGWCC 7 cut(s) 403, 2954, 3206, 4031, 4298, 4368, 5195
Eco47III AGCGCT 2 cut(s) 311, 5269
Eco53kI GAGCTC 1 cut(s) 3630
Eco72I CACGTG 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 3609
Eco88I CYCGRG 1 cut(s) 4581
EcoICRI GAGCTC 1 cut(s) 3630
EcoNI CCTNNNNNAGG 1 cut(s) 942
EcoRI GAATTC 3 cut(s) 1326, 2441, 5006
EcoRII CCWGG 4 cut(s) 3012, 3239, 4099, 4738
EcoRV GATATC 2 cut(s) 2809, 3041
EcoT14I CCWWGG 7 cut(s) 282, 1046, 3261, 3889, 4464, 4712, 5055
EcoT22I ATGCAT 2 cut(s) 2229, 3316
EcoT38I GRGCYC 2 cut(s) 2428, 3632
ErhI CCWWGG 7 cut(s) 282, 1046, 3261, 3889, 4464, 4712, 5055
Esp3I CGTCTC 1 cut(s) 259
FalI AAGNNNNNCTT 6 cut(s) 705, 737, 1683, 1715, 2136, 2168
FaqI GGGAC 8 cut(s) 1271, 1866, 1873, 2284, 3310, 4381, 4485, 4682
FauI CCCGC 4 cut(s) 645, 3685, 3717, 4681
FauNDI CATATG 3 cut(s) 2767, 3700, 5374
FbaI TGATCA 2 cut(s) 694, 1531
FblI GTMKAC 4 cut(s) 555, 1149, 3956, 5368
FriOI GRGCYC 2 cut(s) 2428, 3632
FspBI CTAG 9 cut(s) 1016, 1358, 1724, 1754, 2232, 3105, 3684, 4905, 5454
FspI TGCGCA 1 cut(s) 456
GlaI GCGC 8 cut(s) 311, 321, 332, 370, 456, 788, 1669, 5269
GsaI CCCAGC 2 cut(s) 3589, 5021
GsuI CTGGAG 3 cut(s) 1359, 2412, 5073
HaeII RGCGCY 2 cut(s) 313, 5271
HaeIII GGCC 9 cut(s) 350, 509, 2571, 3012, 3081, 3333, 3682, 3841, 4717
HapII CCGG 7 cut(s) 395, 444, 528, 687, 1202, 3569, 4655
HgaI GACGC 7 cut(s) 262, 360, 388, 431, 572, 2060, 5418
HhaI GCGC 8 cut(s) 312, 322, 333, 371, 457, 789, 1670, 5270
Hin1I GRCGYC 4 cut(s) 252, 380, 423, 564
Hin6I GCGC 8 cut(s) 310, 320, 331, 369, 455, 787, 1668, 5268
HinP1I GCGC 8 cut(s) 310, 320, 331, 369, 455, 787, 1668, 5268
HincII GTYRAC 6 cut(s) 175, 556, 1150, 1288, 4868, 5369
HindII GTYRAC 6 cut(s) 175, 556, 1150, 1288, 4868, 5369
HindIII AAGCTT 5 cut(s) 719, 750, 990, 1706, 3200
HpaI GTTAAC 1 cut(s) 1288
HpaII CCGG 7 cut(s) 395, 444, 528, 687, 1202, 3569, 4655
Hpy99I CGWCG 7 cut(s) 139, 155, 361, 382, 425, 812, 1154
Hsp92I GRCGYC 4 cut(s) 252, 380, 423, 564
HspAI GCGC 8 cut(s) 310, 320, 331, 369, 455, 787, 1668, 5268
Ksp22I TGATCA 2 cut(s) 694, 1531
KspAI GTTAAC 1 cut(s) 1288
KspI CCGCGG 1 cut(s) 501
LguI GCTCTTC 5 cut(s) 300, 978, 2157, 2695, 3637
MabI ACCWGGT 1 cut(s) 4099
MaeI CTAG 9 cut(s) 1016, 1358, 1724, 1754, 2232, 3105, 3684, 4905, 5454
MfeI CAATTG 2 cut(s) 3123, 5172
MflI RGATCY 5 cut(s) 2863, 3547, 4255, 4385, 5200
MhlI GDGCHC 4 cut(s) 2428, 2929, 3632, 4781
MlsI TGGCCA 1 cut(s) 4717
MluNI TGGCCA 1 cut(s) 4717
MmeI TCCRAC 2 cut(s) 4542, 5239
Mox20I TGGCCA 1 cut(s) 4717
Mph1103I ATGCAT 2 cut(s) 2229, 3316
MroXI GAANNNNTTC 3 cut(s) 1124, 3603, 5297
MscI TGGCCA 1 cut(s) 4717
MslI CAYNNNNRTG 8 cut(s) 89, 675, 1947, 2594, 2990, 4161, 4277, 4936
Msp20I TGGCCA 1 cut(s) 4717
MspA1I CMGCKG 8 cut(s) 500, 640, 1220, 1406, 3101, 3692, 3721, 4574
MspCI CTTAAG 2 cut(s) 1877, 3337
MspI CCGG 7 cut(s) 395, 444, 528, 687, 1202, 3569, 4655
MspR9I CCNGG 5 cut(s) 3014, 3241, 3569, 4101, 4740
MunI CAATTG 2 cut(s) 3123, 5172
Mva1269I GAATGC 6 cut(s) 789, 1124, 2464, 4143, 5224, 5352
MvaI CCWGG 4 cut(s) 3014, 3241, 4101, 4740
MvnI CGCG 4 cut(s) 320, 333, 371, 500
NciI CCSGG 1 cut(s) 3569
NdeI CATATG 3 cut(s) 2767, 3700, 5374
NmeAIII GCCGAG 1 cut(s) 592
NmuCI GTSAC 8 cut(s) 372, 542, 658, 797, 1417, 1454, 4900, 5237
NsbI TGCGCA 1 cut(s) 456
NsiI ATGCAT 2 cut(s) 2229, 3316
OliI CACNNNNGTG 1 cut(s) 4277
PaeI GCATGC 2 cut(s) 1946, 2688
PagI TCATGA 2 cut(s) 276, 2311
PasI CCCWGGG 1 cut(s) 4739
PceI AGGCCT 2 cut(s) 3012, 3682
PciI ACATGT 6 cut(s) 670, 1211, 2290, 2948, 3025, 3643
PciSI GCTCTTC 5 cut(s) 300, 978, 2157, 2695, 3637
PcsI WCGNNNNNNNCGW 3 cut(s) 330, 386, 552
PctI GAATGC 6 cut(s) 789, 1124, 2464, 4143, 5224, 5352
PdmI GAANNNNTTC 3 cut(s) 1124, 3603, 5297
PflMI CCANNNNNTGG 2 cut(s) 2201, 5002
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
Ppu21I YACGTR 3 cut(s) 325, 2567, 3221
PscI ACATGT 6 cut(s) 670, 1211, 2290, 2948, 3025, 3643
PshAI GACNNNNGTC 1 cut(s) 284
Psp124BI GAGCTC 1 cut(s) 3632
Psp1406I AACGTT 1 cut(s) 232
Psp6I CCWGG 4 cut(s) 3012, 3239, 4099, 4738
PspCI CACGTG 1 cut(s) 325
PspFI CCCAGC 2 cut(s) 3585, 5017
PspGI CCWGG 4 cut(s) 3012, 3239, 4099, 4738
PspPI GGNCC 8 cut(s) 403, 2954, 3080, 3206, 4031, 4298, 4368, 5195
PstNI CAGNNNCTG 5 cut(s) 2084, 4751, 5024, 5341, 5474
PsuI RGATCY 5 cut(s) 2863, 3547, 4255, 4385, 5200
PvuII CAGCTG 2 cut(s) 1220, 4574
RsaI GTAC 9 cut(s) 933, 2670, 3024, 3223, 3251, 3895, 4881, 4891, 4971
RsaNI GTAC 9 cut(s) 932, 2669, 3023, 3222, 3250, 3894, 4880, 4890, 4970
RseI CAYNNNNRTG 8 cut(s) 89, 675, 1947, 2594, 2990, 4161, 4277, 4936
SacI GAGCTC 1 cut(s) 3632
SacII CCGCGG 1 cut(s) 501
SalI GTCGAC 3 cut(s) 554, 1148, 5367
SapI GCTCTTC 5 cut(s) 300, 978, 2157, 2695, 3637
Sau96I GGNCC 8 cut(s) 403, 2954, 3080, 3206, 4031, 4298, 4368, 5195
ScaI AGTACT 1 cut(s) 3251
ScrFI CCNGG 5 cut(s) 3014, 3241, 3569, 4101, 4740
SduI GDGCHC 4 cut(s) 2428, 2929, 3632, 4781
SexAI ACCWGGT 1 cut(s) 4099
SfcI CTRYAG 6 cut(s) 769, 1977, 3318, 3924, 4047, 4224
Sfr303I CCGCGG 1 cut(s) 501
SgrBI CCGCGG 1 cut(s) 501
SinI GGWCC 7 cut(s) 403, 2954, 3206, 4031, 4298, 4368, 5195
SmiMI CAYNNNNRTG 8 cut(s) 89, 675, 1947, 2594, 2990, 4161, 4277, 4936
SmlI CTYRAG 7 cut(s) 263, 413, 516, 587, 1877, 3245, 3337
SmoI CTYRAG 7 cut(s) 263, 413, 516, 587, 1877, 3245, 3337
SnaBI TACGTA 1 cut(s) 3221
SpeI ACTAGT 1 cut(s) 4904
SphI GCATGC 2 cut(s) 1946, 2688
SseBI AGGCCT 2 cut(s) 3012, 3682
SspI AATATT 2 cut(s) 1912, 4732
SspMI CTAG 9 cut(s) 1016, 1358, 1724, 1754, 2232, 3105, 3684, 4905, 5454
SstI GAGCTC 1 cut(s) 3632
StuI AGGCCT 2 cut(s) 3012, 3682
StyD4I CCNGG 5 cut(s) 3012, 3239, 3567, 4099, 4738
StyI CCWWGG 7 cut(s) 282, 1046, 3261, 3889, 4464, 4712, 5055
TatI WGTACW 3 cut(s) 2668, 3022, 3249
TauI GCSGC 9 cut(s) 331, 503, 506, 509, 3104, 3401, 3709, 3724, 4412
TseFI GTSAC 8 cut(s) 372, 542, 658, 797, 1417, 1454, 4900, 5237
Tsp45I GTSAC 8 cut(s) 372, 542, 658, 797, 1417, 1454, 4900, 5237
TspGWI ACGGA 2 cut(s) 152, 3922
Van91I CCANNNNNTGG 2 cut(s) 2201, 5002
Vha464I CTTAAG 2 cut(s) 1877, 3337
VpaK11BI GGWCC 7 cut(s) 403, 2954, 3206, 4031, 4298, 4368, 5195
XagI CCTNNNNNAGG 1 cut(s) 942
XapI RAATTY 8 cut(s) 1326, 2441, 3186, 3616, 3899, 4351, 5006, 5067
XmiI GTMKAC 4 cut(s) 555, 1149, 3956, 5368
XmnI GAANNNNTTC 3 cut(s) 1124, 3603, 5297
XspI CTAG 9 cut(s) 1016, 1358, 1724, 1754, 2232, 3105, 3684, 4905, 5454
ZraI GACGTC 1 cut(s) 253
ZrmI AGTACT 1 cut(s) 3251
Zsp2I ATGCAT 2 cut(s) 2229, 3316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.