Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005507

Physical Location & Seq

Genomic Coordinates Reverse (-)

21918410 .. 21925954

Gene Structure 7545 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1106000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4812 bp

ATGGGGGATGTTGAAATATCCAATTGGCTCGAAGGGTTGCCATTGGCACCGGTCTTTCGGCCGACGCATACTGAATTCGCTGACCCGATTGCTTATATATCGAAAATCGAAAAGGAAGCTAGTGAATTTGGTATTTGCAGGATTGTCCCACCATTGCCAAAACCCTCAAAAAGATATGTTTTTAGCAACTTGAATAAGTCCCTCTTGAAGTGTCCGGAATTGGGTAGTGATGTAAATTCGTTAGAGGGTTGTTCTCCAACGAGAACGGGTTCTGGGGTTGGTGGTAATGATGGGGAGGCCAGGGCAGTTTTCACTACAAGGCACCAAGAATTGGGGCAGAGTGTGAAAAGTGTGAAAGGGGCAGCAGTTCAGAATCCGCCATCGGGTGTAGATAAGCAAGTGTGGCAAAGTGGGGAAATTTATACTTTGGAGCAGTTTGAAACTAAGGCTAGGGCTTTTGCAAGGAGCATACTAGGATCAGTTAGGGAGGTTACTCCATTGGTTATAGAGGAAATGTTTTGGAAGGCGGCTTCTGAAAAGCCAATATATATTGAGTATGCAAATGATGTGCCTGGGTCTGCTTTTGAAGAGCCAATGGGCCAATTCATCTCTTCTCATAGGCGGAGGAGGAAGAGGAATTCCTACCAGAGAAGTAAAGAAAACTCTGATAGCAAGAAAACTGAAATGATTAGTAGCAGCGAAAGAACCTCCTGTAATGATGAAGTTAAGAATGCATCTCATAGGAATGTTTCGGATACATGTTCAGAGGCATCCAAATCATCTACTGCTCCAGAAATATTTTCAGGTGATGAAACTCCCCAATCATCTAGGCAAAAAAATCCCAATTCATGTTGTGATATGGAAGGTACTGCTGGTTGGAAGCTTTCAAACAGTCCTTGGAACTTACAAGTAATTGCACGTTCACCTGGTTCACTTACTCGTTTCATGCCCGATGATATCCCGGGTGTTACTTCTCCTATGGTTTACATTGGCATGTTGTTTAGTTGGTTTGCTTGGCACGTTGAAGATCATGAGCTTCACAGTATGAATTTTCTTCACACTGGATCTTCAAAGACTTGGTATTCTGTTCCAGGTGATTATGCATTTGCTTTTGAGGAACTTATTCGCACTGAGGCGTTTGGTGGTAATCTCGATCGCCTAGCAGCTGCTCTCTCACTATTGGGCAATAAAACAACTTTAATTTCACCTGAGGCTGTTGTTGCAGCAGGGATTCCTTGTTGCAGAATGTTAATACAGAACCCTGGTGAATTTGTTGTCACTTTTCCAAGGGCTTACCATGTCGGATTCAGCCATGGTTTTAACTGTGGGGAAGCTGCTAACTTTGGAACACCACAATGGCTTAAAGTAGCTAAAGAAGCTGCAGTACGTAGAGCTGCTATGAATTATCTTCCTATGCTCTCCCATCAACAGCTTCTATACCTCTTGACCATGTCTTTTGTTTCGAGGGTGCCAAGGTCCCTACTACCAGGTGTGCGAAGTTCACGTAGGAGAGATCGGCAAAAGGAAGAAAAGGAGCTGTCGGTAAAGAAGGCTTTTATTGAAGATATATTAAAGGAAAACAATGCGTTATCTGTCCTTCTTGGAAAAGAATCAAGTTACCATGCGGTATTATGGAACCATGATTTACTTCCATATACAAGTAAAGATTCCGTAACACCCAGTGGTGGTGCTCCTGTTGATATGAATCCAAAGGAAGATGCTACCCATATTCAGTGTGGAAATTATGATGATCAGAACATTCTGATTGACGAGATGCGTTTGTATATGGAAAATATGAATGATTTGTATTTGGGAAGTGATGATTTATCACAGGACTTTCAAGTCGACTCAGGCACATTGGCATGCGTGGCTTGTGGAATACTTGGGTTTCCATTTATGTCTGTGGTGCAACCATCTGAGAAAGCATCAAAGAAGCTGCAAACTGAAAATTTTATGGCTCAAGGTTCCCCTGGGGTTTCAGGACTTGAAAAGTCTCATTCGTTTCCAGACTGTCACACATCTGTCAAGGGAAATGTTACAGAAGAGGACCTCGTTTCTGTTCCCAACGTTACGCCACCTTCGAATGCTCCCTTAATTCCTTCCACAGCCAAGCTAAAAAAGGGTTGGAATACTGTTAATAAATTTCTGAGACCAAGGAGTTTCTGCCTTGATCATGCTGTTGAAATTGTGGACCTGTTGCAGTGTAAAGGTGGAGCGAACATGCTTGTAATTTGCCATTCTGACTATCAAAAAATAAAGGCTCCTGCTGCAGCAATTGCAGAGGAGATTGGTTGCCCCTTTAAGTATAATGAGGTTCCTTTGGATATCGCGTCAAAGGAAGATCTGAATTTGATAGAGCTTGCAGTTGATGATGAACGTGATGAATGTGGAGAAGACTGGACCTCGATGTTGGGTATCAACCTACGGTACTGTGTCAAGGTCAGAAAGAATTCATCCTCAAAGCAAGTCCAGCATGCATTAACATTGGGTGGCTTGTTCTCCAAACAAAGTCCCAGCTCAGATTTTCTAGCCGTCACATGGCAATCAAGAAGATCTCGATCTAAAAAGCTTAATCATCCAGCGGATTGTACACCATGTGAGAACGTTGAGGAAAAGGATGAAGTTGTGGGCAGAAGGTCAGATGGCACCTCTATTAAGAGGGAAGAAAAAATAATTCAATATTCAAGACGAAAGTCTAAGCCAAAAGCGGGGAGTTCTTCAGGAACAGGTAGGGTCTTTGGGCACCCTGGTACAGGTGTTGAGTGTAGTAAAGAGAGCAGAATGGCAACAGAAAGTAACATTTGTGGCATTGGGAATTCCACAAGCAGTGGTGCAAAACTTGATTTCTCTGCGTCAAGCGGAATGTCTGAAATGTATCCTGTTGTCCAGATGTCTGAAGCAGCGACGGATATCAGTTTGTACTCTACTTCAACACAAGTTTCAGAAACACTAACTGCTGGTAGTGTTGAGGCACAGGTCGAGAATCACAGTTTTGAAGAAAGAAACATGAAAGGTAACGGTTGTAAGTTAGCATCAAGGGATGATTCTGATATGCAAGATGGGATGAGGATTCTTGAAGAAGCCCATGAGAACAAGTCTGCTACTCCACCTGTCATTGCAGCTGATGAAAGTGTTCCAATGCAAATAGAGGAGCAGAAAATGGAAAAATTCAACAATACCAGTGACAATTGTAATCTAGTATCAGAAGGGCAGTCCCAAGTTTTGGCTCATCAGAATGCTCTGATGGATGACGTTTCTGTTCTTGCTGAGACAACAAATTTATGTGCTTCTGACTCAATTGTTACAAGTTCTGAGCCACAGATGGTGAATACAGTCCTGGAGAAATCTTGCATGAGCAATGAAATTTGTGATTCCATGATCTTGGATGAAGTGCAGCAAGAAATCCGAACTACCAACAGAAGTAAAGACAAGACGCCAGTTTCATGTAGTTCCCATAATGATGAAGACTTGAGCAATGATGTGTCTTTAGTATGCAAACTGGAAGAAGAAATTAAGAGTCCGAAAGGAGGAAATGAGGAACCCAGTTTAAGTGGAAGACAAATGAAGGAGCCTTCTCCTGCTTCGGTAGAAGCTACTTCTGGAGTTCCAATAGAGCTGTGTGCTGCAGCAGATTTTTGCTCTGTTCCGGTTTCACATAGTGAAGAATTTGTAACTGCAGATAGAAGCGAGGAGGAATATGTCTCGACTTCAGTGACTCAAATGGAAGTAACTCAACCTTGTATCTCAATGGAAGAATTTTCTGAAGTGCCAAGGGGAGGCTCTTCTGTGGAAGGCACAGATGATGGTGTGAATAATTCAGACGCTCTATTTCAGCAAGAAGTCCAGACTACAAATGGAGCTATAGAGGCACCCATACTTAGTCTCTTCATAAAAACAGAATTGCAGCCCACACCTGGTTTGGTTGAAGAATTTGAGGTTCCCGGAGTGACCTGTGCAACAGATAACATCATTGGTTCTATGGCTTTGGATGGTGAAGAGTGGAAAAACCGTCCCACAAATGGTAATGAGGAACTCATCTCTTGTCAAGATACTTCAAAATGCCAGCCATCTCCTTTTATCCAAACATATTCTAGAGCTAAAAGAAAGGCTAGTGCTGCTCAAGACTTGCGCAATGGTGGTAAAGTTTGCTCATCCCAACATGATAAAGAGATGGAAAACATTGGGTCTTCAAGTGCGGTTGCACCAAGGCTCGAAAACCCTGCACCAAGTCCTGAAATGAGAAGAAAAAGAAAGAGAGGATTGGAGCAAGTAATAGATGACAACCTCGATTCCAATGGATTTATTAGGGGCCCGTGTGAAGGGTTGAGACCAAGGGCTGGGAAGGATGCAACGAGTAGGAATGAGATGGACAACATTTACGAAGAGGTGGAGGAGAAGCCAGTGACGAAGAAGGTGAAGAAACCTTCAGACGTCCATGTTCCTCCCAAGAAAGAACAAGCAAGGAAGTCTCACAGGTGCGACATAGAAAGCTGCCGCATGAGGTTTGCAACAAAGGCGGAGCTAATGTTGCACAAGCGTAACCGTTGCCCACATGAAGGGTGTGGCAAGAGGTTCAGTTCCCACAAGTACGCAGTGAGTCACAGCCGTGTGCATGACGATGATAGGCCCCTCAAGTGCCCGTGGAAAGGCTGCTCCATGTCGTTCAAGTGGGCATGGGCCCGCACTGAGCACATACGCGTGCACACCGGAGAACGGCCTTACCAGTGCAAGGTTGAGGGATGTGGCCTCTTCTTCCGGTTTGTATCGGATTATAGCCGTCACCGACGGAAAACGGGGCATTACGTGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003700 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009826 GO:0009889 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010605 GO:0010629 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0032451 GO:0032452 GO:0032502 GO:0032989 GO:0033169 GO:0033993 GO:0036211 GO:0040007 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045814 GO:0048519 GO:0048577 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048587 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0051276 GO:0060255 GO:0060560 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:0140110 GO:1901564 GO:1901700 GO:1903506 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

JmjC JmjN zf-C2H2

Protein Analysis

1604

Amino Acids

176.95

Weight (kDa)

5.67

Isoelectric Point (pI)

55.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
JmjN	PF02375	17 - 50	9.3e-16	jmjN domain
JmjC	PF02373	328 - 448	1.5e-32	JmjC domain, hydroxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005507)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04240
fragaria_vesca	FvH4_7g19090 FvH4_7g19090 FvH4_7g19090 FvH4_7g19090 FvH4_7g19090 FvH4_7g19090 FvH4_7g19090 FvH4_7g19090 FvH4_7g19090
malus_domestica	MD01G1106000.v1.1 MD07G1172400.v1.1
prunus_persica	Prupe.2G211400_v2.0.a1 Prupe.2G211400_v2.0.a1
pyrus_communis	pycom01g13280 pycom07g16690
rosa_chinensis	RchiOBHm_Chr1g0362971
rosa_laevigata	RLG00000027605
rosa_multiflora	Rmu_sc0000174.1_g000009
rosa_roxburghii	Rroxscaffold_4G00293590
rosa_rugosa	Rorug01G0302900 Rorug01G0303000 Rorug01G0303100 Rorug01G0303200
rosa_samantha	Rh1AG312100 Rh1BG275600 Rh1CG292500 Rh1DG306300
rosa_wichuraiana	Rw1G027660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1841
AatII	GACGTC	1 cut(s)	4434
Acc16I	TGCGCA	1 cut(s)	4130
AccB1I	GGYRCC	6 cut(s)	46, 321, 1468, 2645, 2742, 3868
AccB7I	CCANNNNNTGG	3 cut(s)	331, 3223, 4337
AccI	GTMKAC	1 cut(s)	1845
AccII	CGCG	2 cut(s)	2330, 4698
AccIII	TCCGGA	1 cut(s)	214
AclI	AACGTT	2 cut(s)	2067, 2604
AclWI	GGATC	2 cut(s)	484, 1072
AcoI	YGGCCR	1 cut(s)	59
AcuI	CTGAAG	5 cut(s)	2703, 2916, 3693, 3783, 4410
AcyI	GRCGYC	2 cut(s)	3434, 4431
AdeI	CACNNNGTG	3 cut(s)	1682, 2597, 3659
AfaI	GTAC	7 cut(s)	868, 1386, 2429, 2590, 2752, 2921, 4589
AflIII	ACRYGT	2 cut(s)	758, 4696
AgeI	ACCGGT	1 cut(s)	49
AleI	CACNNNNGTG	1 cut(s)	4605
Alw21I	GWGCWC	3 cut(s)	1693, 4692, 4704
Alw26I	GTCTC	7 cut(s)	1998, 2143, 3263, 3706, 3887, 4321, 4473
Alw44I	GTGCAC	1 cut(s)	4700
AlwI	GGATC	2 cut(s)	484, 1072
Ama87I	CYCGRG	1 cut(s)	961
Aor13HI	TCCGGA	1 cut(s)	214
AoxI	GGCC	8 cut(s)	59, 297, 598, 4309, 4625, 4677, 4715, 4744
ApaI	GGGCCC	2 cut(s)	4313, 4681
ApaLI	GTGCAC	1 cut(s)	4700
AsiGI	ACCGGT	1 cut(s)	49
Asp700I	GAANNNNTTC	4 cut(s)	268, 1122, 2449, 3132
AspLEI	GCGC	1 cut(s)	4131
AsuC2I	CCSGG	3 cut(s)	962, 963, 3942
AsuHPI	GGTGA	9 cut(s)	818, 915, 1106, 1197, 1277, 3337, 4004, 4426, 4772
AsuII	TTCGAA	1 cut(s)	2081
AvaI	CYCGRG	1 cut(s)	961
AvaII	GGWCC	4 cut(s)	1476, 2047, 2191, 2400
AxyI	CCTNAGG	1 cut(s)	1209
BaeGI	GKGCMC	5 cut(s)	2745, 4313, 4640, 4681, 4704
BaeI	ACNNNNGTAYC	2 cut(s)	2742, 2775
BanI	GGYRCC	6 cut(s)	46, 321, 1468, 2645, 2742, 3868
BanII	GRGCYC	2 cut(s)	4313, 4681
BarI	GAAGNNNNNNTAC	2 cut(s)	1368, 1400
BbsI	GAAGAC	4 cut(s)	2400, 3471, 3562, 4179
Bbv12I	GWGCWC	3 cut(s)	1693, 4692, 4704
BceAI	ACGGC	4 cut(s)	2516, 4590, 4730, 4761
BcgI	CGANNNNNNTGC	2 cut(s)	4202, 4236
BciVI	GTATCC	2 cut(s)	748, 2886
BclI	TGATCA	2 cut(s)	1750, 2170
BcnI	CCSGG	3 cut(s)	962, 963, 3942
BcoDI	GTCTC	7 cut(s)	1998, 2143, 3263, 3706, 3887, 4321, 4473
BfaI	CTAG	9 cut(s)	120, 450, 473, 828, 1160, 2528, 3197, 4092, 4110
BfmI	CTRYAG	5 cut(s)	1380, 2268, 3624, 3675, 3861
BfuI	GTATCC	2 cut(s)	748, 2886
BglII	AGATCT	2 cut(s)	2341, 2552
Bme18I	GGWCC	4 cut(s)	1476, 2047, 2191, 2400
BmeT110I	CYCGRG	1 cut(s)	961
BmrI	ACTGGG	2 cut(s)	1674, 3537
BmsI	GCATC	7 cut(s)	743, 779, 1708, 1764, 1934, 3041, 4336
BmuI	ACTGGG	2 cut(s)	1674, 3537
BoxI	GACNNNNGTC	1 cut(s)	2692
BpiI	GAAGAC	4 cut(s)	2400, 3471, 3562, 4179
BpmI	CTGGAG	3 cut(s)	774, 3359, 3621
Bpu14I	TTCGAA	1 cut(s)	2081
BpuEI	CTTGAG	4 cut(s)	1944, 3490, 4104, 4616
BpuMI	CCSGG	3 cut(s)	962, 963, 3942
BsaAI	YACGTR	3 cut(s)	1388, 1505, 4804
BsaBI	GATNNNNATC	2 cut(s)	1704, 2557
BsaHI	GRCGYC	2 cut(s)	3434, 4431
BsaI	GGTCTC	2 cut(s)	2143, 4321
BsaWI	WCCGGW	5 cut(s)	49, 214, 3646, 4706, 4755
BsaXI	ACNNNNNCTCC	2 cut(s)	4511, 4541
Bse118I	RCCGGY	1 cut(s)	49
Bse1I	ACTGG	9 cut(s)	1066, 1680, 2402, 3180, 3437, 3504, 3543, 4400, 4723
Bse21I	CCTNAGG	1 cut(s)	1209
Bse3DI	GCAATG	5 cut(s)	152, 3114, 3364, 3481, 4138
Bse8I	GATNNNNATC	2 cut(s)	1704, 2557
BseAI	TCCGGA	1 cut(s)	214
BseJI	GATNNNNATC	2 cut(s)	1704, 2557
BseMI	GCAATG	5 cut(s)	152, 3114, 3364, 3481, 4138
BseMII	CTCAG	9 cut(s)	1122, 1200, 1863, 1908, 2138, 2532, 3258, 3303, 4677
BseNI	ACTGG	9 cut(s)	1066, 1680, 2402, 3180, 3437, 3504, 3543, 4400, 4723
BseRI	GAGGAG	5 cut(s)	640, 2297, 3164, 3704, 4406
BseSI	GKGCMC	5 cut(s)	2745, 4313, 4640, 4681, 4704
BseX3I	CGGCCG	1 cut(s)	59
BseYI	CCCAGC	2 cut(s)	2513, 4337
BsgI	GTGCAG	2 cut(s)	3413, 4206
Bsh1236I	CGCG	2 cut(s)	2330, 4698
Bsh1285I	CGRYCG	2 cut(s)	62, 1156
BshFI	GGCC	8 cut(s)	61, 299, 600, 4311, 4627, 4679, 4717, 4746
BshNI	GGYRCC	6 cut(s)	46, 321, 1468, 2645, 2742, 3868
BshTI	ACCGGT	1 cut(s)	49
BsiEI	CGRYCG	2 cut(s)	62, 1156
BsiHKAI	GWGCWC	3 cut(s)	1693, 4692, 4704
BsiHKCI	CYCGRG	1 cut(s)	961
BsiSI	CCGG	7 cut(s)	50, 215, 962, 3647, 3942, 4707, 4756
BslFI	GGGAC	6 cut(s)	131, 184, 1462, 2496, 3199, 3996
BsmAI	GTCTC	7 cut(s)	1998, 2143, 3263, 3706, 3887, 4321, 4473
BsmFI	GGGAC	6 cut(s)	131, 184, 1462, 2496, 3199, 3996
BsmI	GAATGC	3 cut(s)	736, 2089, 3241
BsnI	GGCC	8 cut(s)	61, 299, 600, 4311, 4627, 4679, 4717, 4746
Bso31I	GGTCTC	2 cut(s)	2143, 4321
BsoBI	CYCGRG	1 cut(s)	961
Bsp119I	TTCGAA	1 cut(s)	2081
Bsp120I	GGGCCC	2 cut(s)	4309, 4677
Bsp1286I	GDGCHC	7 cut(s)	1693, 2745, 4313, 4640, 4681, 4692, 4704
Bsp13I	TCCGGA	1 cut(s)	214
Bsp1407I	TGTACA	1 cut(s)	2588
Bsp19I	CCATGG	1 cut(s)	1312
BspANI	GGCC	8 cut(s)	61, 299, 600, 4311, 4627, 4679, 4717, 4746
BspCNI	CTCAG	9 cut(s)	1123, 1201, 1862, 1909, 2139, 2531, 3259, 3304, 4678
BspEI	TCCGGA	1 cut(s)	214
BspFNI	CGCG	2 cut(s)	2330, 4698
BspHI	TCATGA	1 cut(s)	1030
BspMAI	CTGCAG	4 cut(s)	1384, 2272, 3628, 3679
BspPI	GGATC	2 cut(s)	484, 1072
BspQI	GCTCTTC	2 cut(s)	582, 3787
BspT104I	TTCGAA	1 cut(s)	2081
BspT107I	GGYRCC	6 cut(s)	46, 321, 1468, 2645, 2742, 3868
BspTNI	GGTCTC	2 cut(s)	2143, 4321
BsrDI	GCAATG	5 cut(s)	152, 3114, 3364, 3481, 4138
BsrFI	RCCGGY	1 cut(s)	49
BsrGI	TGTACA	1 cut(s)	2588
BsrI	ACTGG	9 cut(s)	1066, 1680, 2402, 3180, 3437, 3504, 3543, 4400, 4723
BssAI	RCCGGY	1 cut(s)	49
BssNI	GRCGYC	2 cut(s)	3434, 4431
BssT1I	CCWWGG	8 cut(s)	896, 1286, 1312, 1472, 2153, 3770, 4205, 4331
Bst6I	CTCTTC	9 cut(s)	582, 616, 626, 2037, 3787, 3890, 3990, 4377, 4754
BstACI	GRCGYC	2 cut(s)	3434, 4431
BstAPI	GCANNNNNTGC	1 cut(s)	2276
BstAUI	TGTACA	1 cut(s)	2588
BstBAI	YACGTR	3 cut(s)	1388, 1505, 4804
BstBI	TTCGAA	1 cut(s)	2081
BstC8I	GCNNGC	6 cut(s)	1864, 2361, 2475, 4064, 4681, 4700
BstDSI	CCRYGG	2 cut(s)	1312, 4640
BstENI	CCTNNNNNAGG	1 cut(s)	2751
BstFNI	CGCG	2 cut(s)	2330, 4698
BstHHI	GCGC	1 cut(s)	4131
BstMAI	GTCTC	7 cut(s)	1998, 2143, 3263, 3706, 3887, 4321, 4473
BstMCI	CGRYCG	2 cut(s)	62, 1156
BstNSI	RCATGY	5 cut(s)	762, 997, 1866, 2224, 2477
BstPAI	GACNNNNGTC	1 cut(s)	2692
BstSFI	CTRYAG	5 cut(s)	1380, 2268, 3624, 3675, 3861
BstSLI	GKGCMC	5 cut(s)	2745, 4313, 4640, 4681, 4704
BstSNI	TACGTA	1 cut(s)	1388
BstUI	CGCG	2 cut(s)	2330, 4698
BstV2I	GAAGAC	4 cut(s)	2400, 3471, 3562, 4179
BstX2I	RGATCY	3 cut(s)	1064, 2341, 2552
BstXI	CCANNNNNNTGG	1 cut(s)	3382
BstYI	RGATCY	3 cut(s)	1064, 2341, 2552
BstZI	CGGCCG	1 cut(s)	59
Bsu36I	CCTNAGG	1 cut(s)	1209
BsuI	GTATCC	2 cut(s)	748, 2886
BsuRI	GGCC	8 cut(s)	61, 299, 600, 4311, 4627, 4679, 4717, 4746
BtgI	CCRYGG	2 cut(s)	1312, 4640
BtsI	GCAGTG	3 cut(s)	2207, 2833, 4599
Cac8I	GCNNGC	6 cut(s)	1864, 2361, 2475, 4064, 4681, 4700
CciI	TCATGA	1 cut(s)	1030
CfoI	GCGC	1 cut(s)	4131
Cfr10I	RCCGGY	1 cut(s)	49
Cfr9I	CCCGGG	1 cut(s)	961
CseI	GACGC	5 cut(s)	73, 2319, 2841, 3442, 3830
CsiI	ACCWGGT	3 cut(s)	925, 1486, 3913
Csp6I	GTAC	7 cut(s)	867, 1385, 2428, 2589, 2751, 2920, 4588
CspAI	ACCGGT	1 cut(s)	49
CviQI	GTAC	7 cut(s)	867, 1385, 2428, 2589, 2751, 2920, 4588
DraIII	CACNNNGTG	3 cut(s)	1682, 2597, 3659
DrdI	GACNNNNNNGTC	1 cut(s)	1841
DseDI	GACNNNNNNGTC	1 cut(s)	1841
EaeI	YGGCCR	1 cut(s)	59
EagI	CGGCCG	1 cut(s)	59
Eam1104I	CTCTTC	9 cut(s)	582, 616, 626, 2037, 3787, 3890, 3990, 4377, 4754
EarI	CTCTTC	9 cut(s)	582, 616, 626, 2037, 3787, 3890, 3990, 4377, 4754
EciI	GGCGGA	3 cut(s)	366, 637, 4532
EclXI	CGGCCG	1 cut(s)	59
Eco105I	TACGTA	1 cut(s)	1388
Eco130I	CCWWGG	8 cut(s)	896, 1286, 1312, 1472, 2153, 3770, 4205, 4331
Eco24I	GRGCYC	2 cut(s)	4313, 4681
Eco31I	GGTCTC	2 cut(s)	2143, 4321
Eco32I	GATATC	3 cut(s)	958, 2326, 2911
Eco47I	GGWCC	4 cut(s)	1476, 2047, 2191, 2400
Eco52I	CGGCCG	1 cut(s)	59
Eco57I	CTGAAG	5 cut(s)	2703, 2916, 3693, 3783, 4410
Eco81I	CCTNAGG	1 cut(s)	1209
Eco88I	CYCGRG	1 cut(s)	961
EcoNI	CCTNNNNNAGG	1 cut(s)	2751
EcoO109I	RGGNCCY	4 cut(s)	1476, 2047, 4309, 4626
EcoRI	GAATTC	4 cut(s)	74, 637, 2449, 2815
EcoRV	GATATC	3 cut(s)	958, 2326, 2911
EcoT14I	CCWWGG	8 cut(s)	896, 1286, 1312, 1472, 2153, 3770, 4205, 4331
EcoT22I	ATGCAT	3 cut(s)	736, 1105, 2479
EcoT38I	GRGCYC	2 cut(s)	4313, 4681
ErhI	CCWWGG	8 cut(s)	896, 1286, 1312, 1472, 2153, 3770, 4205, 4331
FalI	AAGNNNNNCTT	2 cut(s)	188, 220
FaqI	GGGAC	6 cut(s)	131, 184, 1462, 2496, 3199, 3996
FauI	CCCGC	2 cut(s)	2701, 4688
FbaI	TGATCA	2 cut(s)	1750, 2170
FblI	GTMKAC	1 cut(s)	1845
FriOI	GRGCYC	2 cut(s)	4313, 4681
FspBI	CTAG	9 cut(s)	120, 450, 473, 828, 1160, 2528, 3197, 4092, 4110
FspI	TGCGCA	1 cut(s)	4130
GlaI	GCGC	1 cut(s)	4130
GsaI	CCCAGC	2 cut(s)	2517, 4341
GsuI	CTGGAG	3 cut(s)	774, 3359, 3621
HaeIII	GGCC	8 cut(s)	61, 299, 600, 4311, 4627, 4679, 4717, 4746
HapII	CCGG	7 cut(s)	50, 215, 962, 3647, 3942, 4707, 4756
HgaI	GACGC	5 cut(s)	73, 2319, 2841, 3442, 3830
HhaI	GCGC	1 cut(s)	4131
Hin1I	GRCGYC	2 cut(s)	3434, 4431
Hin6I	GCGC	1 cut(s)	4129
HinP1I	GCGC	1 cut(s)	4129
HincII	GTYRAC	1 cut(s)	1846
HindII	GTYRAC	1 cut(s)	1846
HindIII	AAGCTT	2 cut(s)	881, 2567
HpaII	CCGG	7 cut(s)	50, 215, 962, 3647, 3942, 4707, 4756
HphI	GGTGA	9 cut(s)	818, 915, 1106, 1197, 1277, 3337, 4004, 4426, 4772
Hpy166II	GTNNAC	8 cut(s)	923, 932, 985, 1502, 1846, 2191, 2591, 4702
Hpy8I	GTNNAC	8 cut(s)	923, 932, 985, 1502, 1846, 2191, 2591, 4702
Hpy99I	CGWCG	3 cut(s)	67, 2908, 4788
Hsp92I	GRCGYC	2 cut(s)	3434, 4431
HspAI	GCGC	1 cut(s)	4129
Kpn2I	TCCGGA	1 cut(s)	214
Ksp22I	TGATCA	2 cut(s)	1750, 2170
LguI	GCTCTTC	2 cut(s)	582, 3787
LweI	GCATC	7 cut(s)	743, 779, 1708, 1764, 1934, 3041, 4336
MabI	ACCWGGT	3 cut(s)	925, 1486, 3913
MaeI	CTAG	9 cut(s)	120, 450, 473, 828, 1160, 2528, 3197, 4092, 4110
MfeI	CAATTG	4 cut(s)	22, 2274, 3187, 3297
MflI	RGATCY	3 cut(s)	1064, 2341, 2552
MhlI	GDGCHC	7 cut(s)	1693, 2745, 4313, 4640, 4681, 4692, 4704
MluI	ACGCGT	1 cut(s)	4696
MlyI	GAGTC	5 cut(s)	1841, 3287, 3526, 3709, 4606
MmeI	TCCRAC	4 cut(s)	281, 857, 1282, 2105
Mph1103I	ATGCAT	3 cut(s)	736, 1105, 2479
MroI	TCCGGA	1 cut(s)	214
MroXI	GAANNNNTTC	4 cut(s)	268, 1122, 2449, 3132
MslI	CAYNNNNRTG	9 cut(s)	744, 992, 1354, 1861, 3234, 3459, 4605, 4617, 4697
MspA1I	CMGCKG	3 cut(s)	1166, 2582, 3122
MspI	CCGG	7 cut(s)	50, 215, 962, 3647, 3942, 4707, 4756
MunI	CAATTG	4 cut(s)	22, 2274, 3187, 3297
Mva1269I	GAATGC	3 cut(s)	736, 2089, 3241
MvnI	CGCG	2 cut(s)	2330, 4698
NciI	CCSGG	3 cut(s)	962, 963, 3942
NcoI	CCATGG	1 cut(s)	1312
NmuCI	GTSAC	9 cut(s)	1276, 2012, 2533, 3182, 3712, 3946, 4402, 4598, 4778
NsbI	TGCGCA	1 cut(s)	4130
NsiI	ATGCAT	3 cut(s)	736, 1105, 2479
NspI	RCATGY	5 cut(s)	762, 997, 1866, 2224, 2477
NspV	TTCGAA	1 cut(s)	2081
OliI	CACNNNNGTG	1 cut(s)	4605
PaeI	GCATGC	2 cut(s)	1866, 2477
PagI	TCATGA	1 cut(s)	1030
PasI	CCCWGGG	1 cut(s)	1970
PciI	ACATGT	1 cut(s)	758
PciSI	GCTCTTC	2 cut(s)	582, 3787
PcsI	WCGNNNNNNNCGW	1 cut(s)	2078
PctI	GAATGC	3 cut(s)	736, 2089, 3241
PdmI	GAANNNNTTC	4 cut(s)	268, 1122, 2449, 3132
PflFI	GACNNNGTC	1 cut(s)	1450
PflMI	CCANNNNNTGG	3 cut(s)	331, 3223, 4337
PfoI	TCCNGGA	2 cut(s)	3336, 3940
PinAI	ACCGGT	1 cut(s)	49
Ple19I	CGATCG	1 cut(s)	1156
PleI	GAGTC	5 cut(s)	1841, 3287, 3525, 3709, 4605
PpsI	GAGTC	5 cut(s)	1841, 3287, 3525, 3709, 4605
Ppu21I	YACGTR	3 cut(s)	1388, 1505, 4804
PpuMI	RGGWCCY	2 cut(s)	1476, 2047
PscI	ACATGT	1 cut(s)	758
PshAI	GACNNNNGTC	1 cut(s)	2692
Psp1406I	AACGTT	2 cut(s)	2067, 2604
Psp5II	RGGWCCY	2 cut(s)	1476, 2047
PspFI	CCCAGC	2 cut(s)	2513, 4337
PspOMI	GGGCCC	2 cut(s)	4309, 4677
PspPPI	RGGWCCY	2 cut(s)	1476, 2047
PstI	CTGCAG	4 cut(s)	1384, 2272, 3628, 3679
PsuI	RGATCY	3 cut(s)	1064, 2341, 2552
PsyI	GACNNNGTC	1 cut(s)	1450
PvuI	CGATCG	1 cut(s)	1156
PvuII	CAGCTG	2 cut(s)	1166, 3122
RsaI	GTAC	7 cut(s)	868, 1386, 2429, 2590, 2752, 2921, 4589
RsaNI	GTAC	7 cut(s)	867, 1385, 2428, 2589, 2751, 2920, 4588
RseI	CAYNNNNRTG	9 cut(s)	744, 992, 1354, 1861, 3234, 3459, 4605, 4617, 4697
SalI	GTCGAC	1 cut(s)	1844
SapI	GCTCTTC	2 cut(s)	582, 3787
SchI	GAGTC	5 cut(s)	1841, 3287, 3526, 3709, 4606
SduI	GDGCHC	7 cut(s)	1693, 2745, 4313, 4640, 4681, 4692, 4704
SexAI	ACCWGGT	3 cut(s)	925, 1486, 3913
SfaNI	GCATC	7 cut(s)	743, 779, 1708, 1764, 1934, 3041, 4336
SfcI	CTRYAG	5 cut(s)	1380, 2268, 3624, 3675, 3861
SfuI	TTCGAA	1 cut(s)	2081
SinI	GGWCC	4 cut(s)	1476, 2047, 2191, 2400
SmaI	CCCGGG	1 cut(s)	963
SmiMI	CAYNNNNRTG	9 cut(s)	744, 992, 1354, 1861, 3234, 3459, 4605, 4617, 4697
SmlI	CTYRAG	4 cut(s)	1959, 3469, 4119, 4631
SmoI	CTYRAG	4 cut(s)	1959, 3469, 4119, 4631
SnaBI	TACGTA	1 cut(s)	1388
SphI	GCATGC	2 cut(s)	1866, 2477
SspI	AATATT	2 cut(s)	798, 2681
SspMI	CTAG	9 cut(s)	120, 450, 473, 828, 1160, 2528, 3197, 4092, 4110
StyI	CCWWGG	8 cut(s)	896, 1286, 1312, 1472, 2153, 3770, 4205, 4331
TatI	WGTACW	2 cut(s)	2588, 2919
TauI	GCSGC	2 cut(s)	530, 4497
TseFI	GTSAC	9 cut(s)	1276, 2012, 2533, 3182, 3712, 3946, 4402, 4598, 4778
Tsp45I	GTSAC	9 cut(s)	1276, 2012, 2533, 3182, 3712, 3946, 4402, 4598, 4778
TspGWI	ACGGA	3 cut(s)	1660, 2921, 4801
TspMI	CCCGGG	1 cut(s)	961
Tth111I	GACNNNGTC	1 cut(s)	1450
Van91I	CCANNNNNTGG	3 cut(s)	331, 3223, 4337
VneI	GTGCAC	1 cut(s)	4700
VpaK11BI	GGWCC	4 cut(s)	1476, 2047, 2191, 2400
XagI	CCTNNNNNAGG	1 cut(s)	2751
XbaI	TCTAGA	1 cut(s)	4091
XceI	RCATGY	5 cut(s)	762, 997, 1866, 2224, 2477
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1733, 3851, 3916
XmaI	CCCGGG	1 cut(s)	961
XmiI	GTMKAC	1 cut(s)	1845
XmnI	GAANNNNTTC	4 cut(s)	268, 1122, 2449, 3132
XspI	CTAG	9 cut(s)	120, 450, 473, 828, 1160, 2528, 3197, 4092, 4110
ZraI	GACGTC	1 cut(s)	4432
Zsp2I	ATGCAT	3 cut(s)	736, 1105, 2479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.