Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004547

Physical Location & Seq

Genomic Coordinates Reverse (-)

22921455 .. 22924222

Gene Structure 2768 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1115000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 918 bp

ATGGCGGCTCTGAAAGTGAGTTTGAGAGGCACTGCAGCTCCTACTTGTTCACCATCACCATCTCTGAAATCTTGGCCGCACATCTCAGATTCTCTCTCCAAGACATCAGCGATAGCCAGATTTCCAAACGCCGCCGTATTGTCTCCTCCCAAGCTTCATCTTCAAGCTTCCCCTGGAAAGAAAACCAGAGCTTCAGTCTCTGCAACTATGGCGACTGGCGGGCAAGAGGTTCTTCCACCAGCTCTTACTTCTACATCGGACCCACCTCCAATCTTCGATGGAAATACAAGGTTGTATATATCTTACCAGTGCCCGTATGCGCAGCGTGCGTGGATTGCCCGGAATTGTAAGGGATTGGAGGAAAAGATACAGTTGGTTCCTATTGATCTGCAAGACAGGCCTGCTTGGTACAAGGAGAAAGTCTACCCTGCTAACAAGGTGCCTTCACTCGAACACAATAACGAAGTGAAAGGAGAGAGTCTTGATTTGATTAGATACATTGACAGTCACTTTGAAGGGCCTTCACTGTTCCCTGATGATCAAGCGAAGAAAGAGTTTGCAGAAGAGTTGCTGTCCTACACAGACACCTTCAATAAATCTGTGTTTTCGTCAATTAAAGAAGATGAAATTGATGCAGCTGGTGCTGCATTTGACTATATCGAAACTGCTCTTTCCAAATTTGAAGATGGACCTTTCTTTCTTGGCAAGTTCAGTCTGGTGGATATAGCTTATGCTCCGTTCCTTGAAAGATTTCAGCCTCTCTTGTTGGACGTGAAGAAGTATGACATCACTGCAGGCAGACCTAAACTGGCAGCATGGATCGAGGAGATGGACAAAAATGTAGCTTACAAACAGACTCGGCGTGATCCCAAAGAGCTTGTCGAAAGTTACAAGAGACGGTTTTTGGCTCAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010731 GO:0016740 GO:0016765 GO:0019538 GO:0034641 GO:0036211 GO:0043170 GO:0043412 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0051186 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C_2 GST_N_3

Protein Analysis

306

Amino Acids

34.08

Weight (kDa)

7.64

Isoelectric Point (pI)

51.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N_3	PF13417	98 - 174	6e-21	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	104 - 169	2.8e-16	Glutathione S-transferase, N-terminal domain
GST_C_5	PF16865	202 - 275	5.5e-07	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	210 - 275	3.3e-12	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004547)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g20610 FvH4_7g20620 FvH4_7g20620
malus_domestica	MD01G1115000.v1.1 MD07G1183000.v1.1
prunus_persica	Prupe.2G221700_v2.0.a1 Prupe.2G221700_v2.0.a1
pyrus_communis	pycom01g14390
rosa_chinensis	RchiOBHm_Chr1g0364651 RchiOBHm_Chr1g0364661
rosa_laevigata	RLG00000027469
rosa_multiflora	Rmu_sc0024218.1_g000001 Rmu_ssc0000112.1_g000036 Rmu_ssc0000112.1_g000037 Rmu_ssc0000282.1_g000016
rosa_roxburghii	Rroxscaffold_1G00029240 Rroxscaffold_4G00291770 Rroxscaffold_4G00292190 Rroxscaffold_7G00185900 Rroxscaffold_7G00191070 Rroxscaffold_7G00191090 Rroxscaffold_7G00197330
rosa_rugosa	Rorug01G0317400
rosa_samantha	Rh1AG326400 Rh1AG326500 Rh1BG287600 Rh1BG287700 Rh1CG304400 Rh1CG304500 Rh1DG319300 Rh1DG319400
rosa_wichuraiana	Rw1G028920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	321
AccB1I	GGYRCC	1 cut(s)	439
AccI	GTMKAC	1 cut(s)	423
AciI	CCGC	4 cut(s)	5, 77, 132, 219
AclWI	GGATC	2 cut(s)	827, 860
AcoI	YGGCCR	1 cut(s)	74
AcsI	RAATTY	1 cut(s)	677
AcuI	CTGAAG	1 cut(s)	177
AfaI	GTAC	1 cut(s)	410
AgsI	TTSAA	5 cut(s)	164, 515, 592, 683, 746
AjiI	CACGTC	1 cut(s)	772
AjnI	CCWGG	1 cut(s)	172
AluBI	AGCT	9 cut(s)	38, 154, 167, 191, 242, 638, 728, 845, 877
AluI	AGCT	9 cut(s)	38, 154, 167, 191, 242, 638, 728, 845, 877
Alw26I	GTCTC	3 cut(s)	147, 202, 889
AlwI	GGATC	2 cut(s)	827, 860
AlwNI	CAGNNNCTG	1 cut(s)	200
AoxI	GGCC	3 cut(s)	74, 398, 518
ApeKI	GCWGC	5 cut(s)	35, 322, 635, 644, 812
ApoI	RAATTY	1 cut(s)	677
ArsI	GACNNNNNNTTYG	4 cut(s)	494, 526, 864, 896
Asp700I	GAANNNNTTC	1 cut(s)	750
AspLEI	GCGC	1 cut(s)	322
AspS9I	GGNCC	3 cut(s)	259, 518, 689
AsuC2I	CCSGG	1 cut(s)	340
AsuHPI	GGTGA	2 cut(s)	42, 48
AvaII	GGWCC	2 cut(s)	259, 689
BaeGI	GKGCMC	1 cut(s)	314
BanI	GGYRCC	1 cut(s)	439
BbvI	GCAGC	5 cut(s)	47, 334, 631, 647, 824
BccI	CCATC	5 cut(s)	61, 67, 272, 680, 823
BceAI	ACGGC	1 cut(s)	119
BciT130I	CCWGG	1 cut(s)	174
BclI	TGATCA	1 cut(s)	538
BcnI	CCSGG	1 cut(s)	340
BcoDI	GTCTC	3 cut(s)	147, 202, 889
BfmI	CTRYAG	2 cut(s)	33, 792
BisI	GCNGC	8 cut(s)	6, 36, 77, 132, 323, 636, 645, 813
BlsI	GCNGC	8 cut(s)	7, 37, 78, 133, 324, 637, 646, 814
Bme1390I	CCNGG	2 cut(s)	174, 340
Bme18I	GGWCC	2 cut(s)	259, 689
BmgBI	CACGTC	1 cut(s)	772
BmgT120I	GGNCC	3 cut(s)	259, 518, 689
BmiI	GGNNCC	3 cut(s)	261, 378, 441
BmrFI	CCNGG	2 cut(s)	174, 340
BmsI	GCATC	1 cut(s)	622
BpuMI	CCSGG	1 cut(s)	340
BsaJI	CCNNGG	1 cut(s)	172
BsaXI	ACNNNNNCTCC	2 cut(s)	22, 52
Bse1I	ACTGG	3 cut(s)	220, 307, 813
BseBI	CCWGG	1 cut(s)	174
BseDI	CCNNGG	1 cut(s)	172
BseMII	CTCAG	1 cut(s)	99
BseNI	ACTGG	3 cut(s)	220, 307, 813
BseRI	GAGGAG	2 cut(s)	135, 839
BseSI	GKGCMC	1 cut(s)	314
BseXI	GCAGC	5 cut(s)	47, 334, 631, 647, 824
BshFI	GGCC	3 cut(s)	76, 400, 520
BshNI	GGYRCC	1 cut(s)	439
BsiSI	CCGG	1 cut(s)	340
BsmAI	GTCTC	3 cut(s)	147, 202, 889
BsmBI	CGTCTC	1 cut(s)	889
BsnI	GGCC	3 cut(s)	76, 400, 520
Bsp1286I	GDGCHC	1 cut(s)	314
Bsp143I	GATC	4 cut(s)	385, 538, 819, 865
BspACI	CCGC	4 cut(s)	5, 77, 132, 219
BspANI	GGCC	3 cut(s)	76, 400, 520
BspCNI	CTCAG	1 cut(s)	98
BspLI	GGNNCC	3 cut(s)	261, 378, 441
BspMAI	CTGCAG	2 cut(s)	37, 796
BspPI	GGATC	2 cut(s)	827, 860
BspT107I	GGYRCC	1 cut(s)	439
BsrI	ACTGG	3 cut(s)	220, 307, 813
BssECI	CCNNGG	1 cut(s)	172
BssMI	GATC	4 cut(s)	385, 538, 819, 865
Bst2UI	CCWGG	1 cut(s)	174
Bst4CI	ACNGT	4 cut(s)	372, 506, 528, 900
Bst6I	CTCTTC	1 cut(s)	558
BstAPI	GCANNNNNTGC	1 cut(s)	641
BstC8I	GCNNGC	4 cut(s)	221, 327, 402, 796
BstDEI	CTNAG	2 cut(s)	85, 909
BstHHI	GCGC	1 cut(s)	322
BstKTI	GATC	4 cut(s)	388, 541, 822, 868
BstMAI	GTCTC	3 cut(s)	147, 202, 889
BstMBI	GATC	4 cut(s)	385, 538, 819, 865
BstMWI	GCNNNNNNNGC	6 cut(s)	209, 326, 335, 397, 641, 644
BstNI	CCWGG	1 cut(s)	174
BstSCI	CCNGG	2 cut(s)	172, 338
BstSFI	CTRYAG	2 cut(s)	33, 792
BstSLI	GKGCMC	1 cut(s)	314
BstV1I	GCAGC	5 cut(s)	47, 334, 631, 647, 824
BsuRI	GGCC	3 cut(s)	76, 400, 520
BtrI	CACGTC	1 cut(s)	772
BtsI	GCAGTG	2 cut(s)	30, 789
BtsIMutI	CAGTG	4 cut(s)	30, 314, 524, 789
Cac8I	GCNNGC	4 cut(s)	221, 327, 402, 796
CaiI	CAGNNNCTG	1 cut(s)	200
CfoI	GCGC	1 cut(s)	322
Cfr13I	GGNCC	3 cut(s)	259, 518, 689
Csp6I	GTAC	1 cut(s)	409
CviAII	CATG	1 cut(s)	816
CviQI	GTAC	1 cut(s)	409
DdeI	CTNAG	2 cut(s)	85, 909
DpnI	GATC	4 cut(s)	387, 540, 821, 867
DpnII	GATC	4 cut(s)	385, 538, 819, 865
EaeI	YGGCCR	1 cut(s)	74
Eam1104I	CTCTTC	1 cut(s)	558
EarI	CTCTTC	1 cut(s)	558
Eco147I	AGGCCT	1 cut(s)	400
Eco47I	GGWCC	2 cut(s)	259, 689
Eco57I	CTGAAG	1 cut(s)	177
EcoO109I	RGGNCCY	1 cut(s)	518
EcoRII	CCWGG	1 cut(s)	172
Esp3I	CGTCTC	1 cut(s)	889
FaeI	CATG	1 cut(s)	819
FaiI	YATR	9 cut(s)	209, 297, 299, 318, 657, 725, 732, 783, 817
FalI	AAGNNNNNCTT	2 cut(s)	216, 248
FatI	CATG	1 cut(s)	815
FauI	CCCGC	1 cut(s)	212
FbaI	TGATCA	1 cut(s)	538
FblI	GTMKAC	1 cut(s)	423
Fnu4HI	GCNGC	8 cut(s)	6, 36, 77, 132, 323, 636, 645, 813
Fsp4HI	GCNGC	8 cut(s)	6, 36, 77, 132, 323, 636, 645, 813
FspI	TGCGCA	1 cut(s)	321
GlaI	GCGC	1 cut(s)	321
GluI	GCNGC	8 cut(s)	6, 36, 77, 132, 323, 636, 645, 813
HaeIII	GGCC	3 cut(s)	76, 400, 520
HapII	CCGG	1 cut(s)	340
HhaI	GCGC	1 cut(s)	322
Hin1II	CATG	1 cut(s)	819
Hin6I	GCGC	1 cut(s)	320
HinP1I	GCGC	1 cut(s)	320
HindIII	AAGCTT	2 cut(s)	152, 165
HinfI	GANTC	3 cut(s)	89, 478, 856
HpaII	CCGG	1 cut(s)	340
HphI	GGTGA	2 cut(s)	42, 48
Hpy166II	GTNNAC	2 cut(s)	50, 424
Hpy188I	TCNGA	5 cut(s)	12, 66, 88, 259, 912
Hpy188III	TCNNGA	1 cut(s)	482
Hpy8I	GTNNAC	2 cut(s)	50, 424
HpyAV	CCTTC	4 cut(s)	453, 509, 531, 598
HpyCH4III	ACNGT	4 cut(s)	372, 506, 528, 900
HpyCH4IV	ACGT	1 cut(s)	771
HpyCH4V	TGCA	7 cut(s)	35, 203, 391, 560, 635, 647, 794
HpyF10VI	GCNNNNNNNGC	6 cut(s)	209, 326, 335, 397, 641, 644
HpyF3I	CTNAG	2 cut(s)	85, 909
HpySE526I	ACGT	1 cut(s)	771
Hsp92II	CATG	1 cut(s)	819
HspAI	GCGC	1 cut(s)	320
Ksp22I	TGATCA	1 cut(s)	538
Kzo9I	GATC	4 cut(s)	385, 538, 819, 865
LmnI	GCTCC	2 cut(s)	43, 739
Lsp1109I	GCAGC	5 cut(s)	47, 334, 631, 647, 824
LweI	GCATC	1 cut(s)	622
MaeII	ACGT	1 cut(s)	771
MaeIII	GTNAC	2 cut(s)	506, 887
MalI	GATC	4 cut(s)	387, 540, 821, 867
MboI	GATC	4 cut(s)	385, 538, 819, 865
MboII	GAAGA	8 cut(s)	152, 224, 265, 559, 575, 632, 695, 787
MhlI	GDGCHC	1 cut(s)	314
MluCI	AATT	4 cut(s)	343, 612, 627, 677
MlyI	GAGTC	2 cut(s)	487, 850
MmeI	TCCRAC	1 cut(s)	747
MnlI	CCTC	7 cut(s)	20, 156, 220, 276, 352, 768, 817
MroXI	GAANNNNTTC	1 cut(s)	750
MseI	TTAA	1 cut(s)	615
MspA1I	CMGCKG	1 cut(s)	638
MspI	CCGG	1 cut(s)	340
MspR9I	CCNGG	2 cut(s)	174, 340
MvaI	CCWGG	1 cut(s)	174
MwoI	GCNNNNNNNGC	6 cut(s)	209, 326, 335, 397, 641, 644
NciI	CCSGG	1 cut(s)	340
NdeII	GATC	4 cut(s)	385, 538, 819, 865
NlaIII	CATG	1 cut(s)	819
NlaIV	GGNNCC	3 cut(s)	261, 378, 441
NmeAIII	GCCGAG	1 cut(s)	838
NmuCI	GTSAC	1 cut(s)	506
NsbI	TGCGCA	1 cut(s)	321
PceI	AGGCCT	1 cut(s)	400
PdmI	GAANNNNTTC	1 cut(s)	750
PfeI	GAWTC	1 cut(s)	89
PkrI	GCNGC	8 cut(s)	7, 37, 78, 133, 324, 637, 646, 814
PleI	GAGTC	2 cut(s)	486, 850
PpsI	GAGTC	2 cut(s)	486, 850
Psp6I	CCWGG	1 cut(s)	172
PspGI	CCWGG	1 cut(s)	172
PspN4I	GGNNCC	3 cut(s)	261, 378, 441
PspPI	GGNCC	3 cut(s)	259, 518, 689
PstI	CTGCAG	2 cut(s)	37, 796
PstNI	CAGNNNCTG	1 cut(s)	200
PvuII	CAGCTG	1 cut(s)	638
RsaI	GTAC	1 cut(s)	410
RsaNI	GTAC	1 cut(s)	409
SaqAI	TTAA	1 cut(s)	615
SatI	GCNGC	8 cut(s)	6, 36, 77, 132, 323, 636, 645, 813
Sau3AI	GATC	4 cut(s)	385, 538, 819, 865
Sau96I	GGNCC	3 cut(s)	259, 518, 689
SchI	GAGTC	2 cut(s)	487, 850
ScrFI	CCNGG	2 cut(s)	174, 340
SduI	GDGCHC	1 cut(s)	314
SfaNI	GCATC	1 cut(s)	622
SfcI	CTRYAG	2 cut(s)	33, 792
SinI	GGWCC	2 cut(s)	259, 689
Sse9I	AATT	4 cut(s)	343, 612, 627, 677
SseBI	AGGCCT	1 cut(s)	400
SsiI	CCGC	4 cut(s)	5, 77, 132, 219
StuI	AGGCCT	1 cut(s)	400
StyD4I	CCNGG	2 cut(s)	172, 338
TaaI	ACNGT	4 cut(s)	372, 506, 528, 900
TaiI	ACGT	1 cut(s)	774
TaqI	TCGA	5 cut(s)	276, 450, 660, 822, 882
TasI	AATT	4 cut(s)	343, 612, 627, 677
TauI	GCSGC	3 cut(s)	8, 79, 134
TfiI	GAWTC	1 cut(s)	89
Tru1I	TTAA	1 cut(s)	615
Tru9I	TTAA	1 cut(s)	615
TscAI	CASTG	4 cut(s)	37, 314, 531, 796
TseFI	GTSAC	1 cut(s)	506
TseI	GCWGC	5 cut(s)	35, 322, 635, 644, 812
Tsp45I	GTSAC	1 cut(s)	506
TspDTI	ATGAA	2 cut(s)	146, 639
TspGWI	ACGGA	1 cut(s)	726
TspRI	CASTG	4 cut(s)	37, 314, 531, 796
VpaK11BI	GGWCC	2 cut(s)	259, 689
XapI	RAATTY	1 cut(s)	677
XmiI	GTMKAC	1 cut(s)	423
XmnI	GAANNNNTTC	1 cut(s)	750

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.