RchiOBHm_Chr1g0364661

Glutathione S-transferase, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004547
Physical Location & Seq
Reverse (-)
55756616 .. 55759436
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58934

Sequence Viewer

Length: 891 bp
ATGACTTCCACTTTGAAAGTCAGTTTCAGAGGCGTTGTACCTACTTCATCACCTCTGAAGTCTCGACCGCGCGTTTTAGATTCATTGTATGTAGCCAAGTTTCCAAACACCACCATACTATGTCCTCCTAAGCTTCGACTTCAAGCTAAAACAACCAGAGCTTCACTCTCTGCAACAATGGCAACTGGTGTGCAAGAGGCTCTTCCACCAGCTCTTACTTCTACCTCAGACCCTCCTTCAATCTTCGATGGGAAAACAAGGTTGTATATATCTTACACATGCCCATTTGCACAGCGTGCGTGGATTGCCCGGAACTGCAAGGGATTGGAGGAAAAGATAGAATTGGTTCCTATTAATCTGCAAGACAGGCCTTCTTGGTACAAGGAGAAAGTCTACCCTCCTAACAAGGTGCCATCACTGGAACACAATAACGAAGTCAAAGGAGAGAGTCTTGATTTGATTAGATACATCGACAGTAACTTTGAAGGGCCTTCACTGTTCCCTGATGATCCTGCAAAGAGAGAGTTTGCAGAAGAGTTGTTTACCTACACCGACTCCTTCAGCAAACCTGTGATTTCCTTTTTTAAAGGAGAAGGAACTGAGGCAGCTGCTGGTGCTGCATTTGACTATATTGAAACTGCTCTTGCCAAATTTGAAGATGGACCTTTCTTTCTTGGCCAATTCAGTCTGGTGGATATCGCTTATGCACCATTCATTGATAGATTTCAGCATTTCGCATTGGATGTGAAGAAATATGACATCACTGCAGGAAGACCTAAACTGGCAGCATGGATTGAGGAGATGAACAAAAATGAAGGTTTCAATAAGACCCGACGTGATCCCAAAGAGCTCGTTGAAAGCTACAAGAAACGCTTTTCGGCTAAGGTCTGA

Protein Analysis

296

Amino Acids

33.35

Weight (kDa)

8.75

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_3 PF13417 88 - 164 2.6e-21 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 93 - 159 1.6e-15 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 202 - 266 3.1e-11 Glutathione S-transferase, C-terminal domain
GST_C PF00043 203 - 270 3.8e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 409
AccI GTMKAC 1 cut(s) 393
AccII CGCG 2 cut(s) 70, 72
AciI CCGC 1 cut(s) 68
AclWI GGATC 2 cut(s) 503, 833
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 2 cut(s) 77, 544
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 2 cut(s) 39, 380
AgsI TTSAA 8 cut(s) 16, 143, 240, 485, 635, 656, 823, 857
AjiI CACGTC 1 cut(s) 836
AluBI AGCT 7 cut(s) 133, 146, 161, 212, 608, 850, 861
AluI AGCT 7 cut(s) 133, 146, 161, 212, 608, 850, 861
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 1 cut(s) 66
AlwI GGATC 2 cut(s) 503, 833
AlwNI CAGNNNCTG 1 cut(s) 611
AoxI GGCC 3 cut(s) 368, 488, 676
ApeKI GCWGC 4 cut(s) 605, 608, 617, 785
ApoI RAATTY 1 cut(s) 650
ArsI GACNNNNNNTTYG 3 cut(s) 27, 464, 496
AseI ATTAAT 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 345
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 2 cut(s) 488, 662
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 42
AvaII GGWCC 1 cut(s) 662
BalI TGGCCA 1 cut(s) 678
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 778
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 4 cut(s) 595, 604, 617, 797
BccI CCATC 3 cut(s) 242, 421, 653
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 66
BfmI CTRYAG 1 cut(s) 765
BisI GCNGC 4 cut(s) 606, 609, 618, 786
BlsI GCNGC 4 cut(s) 607, 610, 619, 787
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 1 cut(s) 662
BmgBI CACGTC 1 cut(s) 836
BmgT120I GGNCC 2 cut(s) 488, 662
BmiI GGNNCC 2 cut(s) 348, 411
BmrFI CCNGG 1 cut(s) 310
BpiI GAAGAC 1 cut(s) 778
BplI GAGNNNNNCTC 2 cut(s) 150, 182
Bpu10I CCTNAGC 2 cut(s) 129, 882
BpuMI CCSGG 1 cut(s) 310
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bse1I ACTGG 3 cut(s) 190, 423, 786
BseGI GGATG 1 cut(s) 748
BseMII CTCAG 2 cut(s) 240, 591
BseNI ACTGG 3 cut(s) 190, 423, 786
BseRI GAGGAG 1 cut(s) 812
BseXI GCAGC 4 cut(s) 595, 604, 617, 797
Bsh1236I CGCG 2 cut(s) 70, 72
Bsh1285I CGRYCG 1 cut(s) 68
BshFI GGCC 3 cut(s) 370, 490, 678
BshNI GGYRCC 1 cut(s) 409
BsiEI CGRYCG 1 cut(s) 68
BsiHKAI GWGCWC 1 cut(s) 852
BsiSI CCGG 1 cut(s) 310
BsmAI GTCTC 1 cut(s) 66
BsnI GGCC 3 cut(s) 370, 490, 678
Bsp1286I GDGCHC 1 cut(s) 852
Bsp143I GATC 2 cut(s) 508, 838
BspACI CCGC 1 cut(s) 68
BspANI GGCC 3 cut(s) 370, 490, 678
BspCNI CTCAG 2 cut(s) 239, 592
BspFNI CGCG 2 cut(s) 70, 72
BspLI GGNNCC 2 cut(s) 348, 411
BspMAI CTGCAG 1 cut(s) 769
BspPI GGATC 2 cut(s) 503, 833
BspQI GCTCTTC 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 409
BsrI ACTGG 3 cut(s) 190, 423, 786
BssMI GATC 2 cut(s) 508, 838
Bst4CI ACNGT 2 cut(s) 476, 498
Bst6I CTCTTC 2 cut(s) 207, 528
BstAPI GCANNNNNTGC 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 4 cut(s) 129, 226, 600, 882
BstF5I GGATG 1 cut(s) 748
BstFNI CGCG 2 cut(s) 70, 72
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 2 cut(s) 511, 841
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 2 cut(s) 508, 838
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 6 cut(s) 179, 296, 305, 367, 614, 617
BstNSI RCATGY 1 cut(s) 282
BstSCI CCNGG 1 cut(s) 308
BstSFI CTRYAG 1 cut(s) 765
BstUI CGCG 2 cut(s) 70, 72
BstV1I GCAGC 4 cut(s) 595, 604, 617, 797
BstV2I GAAGAC 1 cut(s) 778
BsuRI GGCC 3 cut(s) 370, 490, 678
BtrI CACGTC 1 cut(s) 836
BtsCI GGATG 1 cut(s) 748
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 3 cut(s) 416, 494, 762
Cac8I GCNNGC 1 cut(s) 297
CaiI CAGNNNCTG 1 cut(s) 611
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 2 cut(s) 488, 662
Csp6I GTAC 2 cut(s) 38, 379
CviAII CATG 2 cut(s) 279, 789
CviQI GTAC 2 cut(s) 38, 379
DdeI CTNAG 4 cut(s) 129, 226, 600, 882
DpnI GATC 2 cut(s) 510, 840
DpnII GATC 2 cut(s) 508, 838
DraI TTTAAA 1 cut(s) 586
DraIII CACNNNGTG 1 cut(s) 296
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 2 cut(s) 207, 528
EarI CTCTTC 2 cut(s) 207, 528
Ecl136II GAGCTC 1 cut(s) 850
Eco147I AGGCCT 1 cut(s) 370
Eco24I GRGCYC 1 cut(s) 852
Eco32I GATATC 1 cut(s) 697
Eco47I GGWCC 1 cut(s) 662
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 2 cut(s) 77, 544
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 488
EcoRV GATATC 1 cut(s) 697
EcoT38I GRGCYC 1 cut(s) 852
FaeI CATG 2 cut(s) 282, 792
FalI AAGNNNNNCTT 4 cut(s) 186, 218, 857, 889
FatI CATG 2 cut(s) 278, 788
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 4 cut(s) 606, 609, 618, 786
FokI GGATG 1 cut(s) 755
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 4 cut(s) 606, 609, 618, 786
GlaI GCGC 1 cut(s) 71
GluI GCNGC 4 cut(s) 606, 609, 618, 786
HaeIII GGCC 3 cut(s) 370, 490, 678
HapII CCGG 1 cut(s) 310
HhaI GCGC 1 cut(s) 72
Hin1II CATG 2 cut(s) 282, 792
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 3 cut(s) 80, 448, 554
HpaII CCGG 1 cut(s) 310
HphI GGTGA 1 cut(s) 42
Hpy166II GTNNAC 2 cut(s) 394, 543
Hpy188I TCNGA 4 cut(s) 29, 57, 229, 890
Hpy188III TCNNGA 2 cut(s) 63, 452
Hpy8I GTNNAC 2 cut(s) 394, 543
Hpy99I CGWCG 1 cut(s) 837
HpyAV CCTTC 7 cut(s) 246, 381, 479, 501, 568, 587, 809
HpyCH4III ACNGT 2 cut(s) 476, 498
HpyCH4IV ACGT 1 cut(s) 835
HpyF10VI GCNNNNNNNGC 6 cut(s) 179, 296, 305, 367, 614, 617
HpyF3I CTNAG 4 cut(s) 129, 226, 600, 882
HpySE526I ACGT 1 cut(s) 835
Hsp92II CATG 2 cut(s) 282, 792
HspAI GCGC 1 cut(s) 70
Kzo9I GATC 2 cut(s) 508, 838
LguI GCTCTTC 1 cut(s) 207
Lsp1109I GCAGC 4 cut(s) 595, 604, 617, 797
MaeII ACGT 1 cut(s) 835
MaeIII GTNAC 1 cut(s) 476
MalI GATC 2 cut(s) 510, 840
MboI GATC 2 cut(s) 508, 838
MboII GAAGA 6 cut(s) 194, 235, 545, 668, 760, 783
MhlI GDGCHC 1 cut(s) 852
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 3 cut(s) 341, 650, 680
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 2 cut(s) 457, 548
Mox20I TGGCCA 1 cut(s) 678
MroXI GAANNNNTTC 1 cut(s) 345
MscI TGGCCA 1 cut(s) 678
MseI TTAA 2 cut(s) 354, 585
Msp20I TGGCCA 1 cut(s) 678
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 1 cut(s) 310
MspR9I CCNGG 1 cut(s) 310
MvnI CGCG 2 cut(s) 70, 72
MwoI GCNNNNNNNGC 6 cut(s) 179, 296, 305, 367, 614, 617
NciI CCSGG 1 cut(s) 310
NdeII GATC 2 cut(s) 508, 838
NlaIII CATG 2 cut(s) 282, 792
NlaIV GGNNCC 2 cut(s) 348, 411
NspI RCATGY 1 cut(s) 282
PceI AGGCCT 1 cut(s) 370
PciSI GCTCTTC 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 4 cut(s) 607, 610, 619, 787
PleI GAGTC 2 cut(s) 456, 548
PpsI GAGTC 2 cut(s) 456, 548
PshBI ATTAAT 1 cut(s) 354
Psp124BI GAGCTC 1 cut(s) 852
PspN4I GGNNCC 2 cut(s) 348, 411
PspPI GGNCC 2 cut(s) 488, 662
PstI CTGCAG 1 cut(s) 769
PstNI CAGNNNCTG 1 cut(s) 611
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 2 cut(s) 39, 380
RsaNI GTAC 2 cut(s) 38, 379
SacI GAGCTC 1 cut(s) 852
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 2 cut(s) 354, 585
SatI GCNGC 4 cut(s) 606, 609, 618, 786
Sau3AI GATC 2 cut(s) 508, 838
Sau96I GGNCC 2 cut(s) 488, 662
SchI GAGTC 2 cut(s) 457, 548
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 1 cut(s) 852
SfcI CTRYAG 1 cut(s) 765
SinI GGWCC 1 cut(s) 662
Sse9I AATT 3 cut(s) 341, 650, 680
SseBI AGGCCT 1 cut(s) 370
SsiI CCGC 1 cut(s) 68
SstI GAGCTC 1 cut(s) 852
StuI AGGCCT 1 cut(s) 370
StyD4I CCNGG 1 cut(s) 308
TaaI ACNGT 2 cut(s) 476, 498
TaiI ACGT 1 cut(s) 838
TaqI TCGA 4 cut(s) 64, 136, 246, 471
TasI AATT 3 cut(s) 341, 650, 680
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 2 cut(s) 354, 585
Tru9I TTAA 2 cut(s) 354, 585
TscAI CASTG 3 cut(s) 423, 501, 769
TseI GCWGC 4 cut(s) 605, 608, 617, 785
TspDTI ATGAA 5 cut(s) 36, 72, 703, 818, 828
TspRI CASTG 3 cut(s) 423, 501, 769
VpaK11BI GGWCC 1 cut(s) 662
VspI ATTAAT 1 cut(s) 354
XapI RAATTY 1 cut(s) 650
XceI RCATGY 1 cut(s) 282
XmiI GTMKAC 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.