MD01G1124200.v1.1

Enoyl-CoA hydratase/isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008739
Physical Location & Seq
Reverse (-)
23720805 .. 23721521
717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1124200.v1.1.491

Sequence Viewer

Length: 717 bp
ATGTGTACGTTAGAGAGGAAAGGCAACATCTTCATCCTAACGCTAACAGGCCCAGGCGAGCACAGGGTCAACCCCACTCTCATCGACTCCATCCGATCCGCCCTCAACCAAGTCCGAGCCGCCGTCACCACCACGGCCTCGTCATCATCTCCCTCAGCCCTAATCACCACCGCACATGGCAAATTCTTCTCCAACGGCTACGATCTCTCTTGGGCCCAGTCCTCTGAATCCCGCATAGAGCTCATGGACGCCAAGCTCCGGTCCCTTGTCGCCGATCTCATCTCCCTCCCGATGCCCACCATCGCCGCCGTATCCGGCCACGCCTCCGCCGCCGGCTTCATCCTCGCTCTCAGCCACGACTACCTTCTCATGAGGAGGGACCGCGGCTTCATCTACATGAGCGAGCTCGACATCGAGCTCCTTCTGCCACGCTGGTTCTTGGCGCTGATCGAGAGCAAGGTCGGCTCGCCGGCGGCTCGGCGCGACTTGTTGCTCAGAGCCGATAAGGTGAAAGCCGACGTGGCAGTGGCGAAGGGGATTATCTACTCGGCGCACGATAGCGCGGAGGAGACCGTCGAGGCCGCGGCTAATTTGGGGGAGGAGTTGGTTCGGCGGAAGTGGAACGGACACGTGTACGCTCATATTCGGAAGGACTTGTTGTCTGATGTTCTGGATGAGATCCGCAGAAATACTACTGGCAGAAAATCACGACTGTAG

Protein Analysis

239

Amino Acids

26.17

Weight (kDa)

9.04

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 7 - 205 1.7e-17 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 384, 483, 563, 584
AclWI GGATC 2 cut(s) 90, 673
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 1 cut(s) 182
AcvI CACGTG 1 cut(s) 631
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 2 cut(s) 7, 635
AfiI CCNNNNNNNGG 1 cut(s) 258
AflIII ACRYGT 2 cut(s) 628, 630
AhdI GACNNNNNGTC 1 cut(s) 658
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 4 cut(s) 241, 256, 406, 418
AluI AGCT 4 cut(s) 241, 256, 406, 418
Alw21I GWGCWC 4 cut(s) 63, 243, 408, 420
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 2 cut(s) 90, 673
AoxI GGCC 5 cut(s) 49, 135, 213, 316, 579
ApaI GGGCCC 1 cut(s) 217
ApoI RAATTY 1 cut(s) 182
AspLEI GCGC 4 cut(s) 445, 483, 553, 563
AspS9I GGNCC 5 cut(s) 50, 213, 214, 261, 379
AsuHPI GGTGA 3 cut(s) 118, 157, 520
AvaII GGWCC 2 cut(s) 261, 379
BaeGI GKGCMC 1 cut(s) 217
BanII GRGCYC 4 cut(s) 217, 243, 408, 420
BbrPI CACGTG 1 cut(s) 631
Bbv12I GWGCWC 4 cut(s) 63, 243, 408, 420
BbvCI CCTCAGC 1 cut(s) 154
BccI CCATC 2 cut(s) 98, 308
BceAI ACGGC 4 cut(s) 107, 150, 211, 293
BciT130I CCWGG 1 cut(s) 54
BciVI GTATCC 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 563
BfmI CTRYAG 1 cut(s) 713
BfoI RGCGCY 1 cut(s) 446
BfuI GTATCC 1 cut(s) 322
BglI GCCNNNNNGGC 1 cut(s) 521
BisI GCNGC 7 cut(s) 120, 306, 330, 385, 474, 582, 585
BlsI GCNGC 7 cut(s) 121, 307, 331, 386, 475, 583, 586
Bme1390I CCNGG 1 cut(s) 54
Bme18I GGWCC 2 cut(s) 261, 379
BmeRI GACNNNNNGTC 1 cut(s) 658
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 5 cut(s) 50, 213, 214, 261, 379
BmiI GGNNCC 3 cut(s) 215, 263, 380
BmrFI CCNGG 1 cut(s) 54
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 1 cut(s) 282
BmuI ACTGGG 1 cut(s) 211
Bpu10I CCTNAGC 1 cut(s) 154
BsaAI YACGTR 1 cut(s) 631
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 4 cut(s) 52, 132, 382, 582
BsaWI WCCGGW 1 cut(s) 258
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse118I RCCGGY 2 cut(s) 332, 469
Bse1I ACTGG 2 cut(s) 217, 700
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 4 cut(s) 52, 132, 382, 582
BseGI GGATG 4 cut(s) 33, 90, 339, 679
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 3 cut(s) 168, 364, 508
BseNI ACTGG 2 cut(s) 217, 700
BseRI GAGGAG 3 cut(s) 388, 581, 614
BseSI GKGCMC 1 cut(s) 217
Bsh1236I CGCG 4 cut(s) 384, 483, 563, 584
BshFI GGCC 5 cut(s) 51, 137, 215, 318, 581
BsiHKAI GWGCWC 4 cut(s) 63, 243, 408, 420
BsiSI CCGG 4 cut(s) 259, 315, 333, 470
BslFI GGGAC 2 cut(s) 247, 392
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 1 cut(s) 563
BsmFI GGGAC 2 cut(s) 247, 392
BsnI GGCC 5 cut(s) 51, 137, 215, 318, 581
Bso31I GGTCTC 1 cut(s) 563
Bsp120I GGGCCC 1 cut(s) 213
Bsp1286I GDGCHC 5 cut(s) 63, 217, 243, 408, 420
Bsp143I GATC 5 cut(s) 95, 202, 274, 447, 678
BspANI GGCC 5 cut(s) 51, 137, 215, 318, 581
BspCNI CTCAG 3 cut(s) 167, 363, 507
BspFNI CGCG 4 cut(s) 384, 483, 563, 584
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 3 cut(s) 215, 263, 380
BspPI GGATC 2 cut(s) 90, 673
BspTNI GGTCTC 1 cut(s) 563
BsrFI RCCGGY 2 cut(s) 332, 469
BsrI ACTGG 2 cut(s) 217, 700
BssAI RCCGGY 2 cut(s) 332, 469
BssECI CCNNGG 4 cut(s) 52, 132, 382, 582
BssMI GATC 5 cut(s) 95, 202, 274, 447, 678
BssNI GRCGYC 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 2 cut(s) 574, 714
BstACI GRCGYC 1 cut(s) 249
BstBAI YACGTR 1 cut(s) 631
BstC8I GCNNGC 5 cut(s) 59, 334, 404, 467, 471
BstDEI CTNAG 3 cut(s) 154, 350, 494
BstDSI CCRYGG 3 cut(s) 132, 382, 582
BstF5I GGATG 4 cut(s) 33, 90, 339, 679
BstFNI CGCG 4 cut(s) 384, 483, 563, 584
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 4 cut(s) 445, 483, 553, 563
BstKTI GATC 5 cut(s) 98, 205, 277, 450, 681
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 5 cut(s) 95, 202, 274, 447, 678
BstMWI GCNNNNNNNGC 4 cut(s) 329, 424, 462, 521
BstNI CCWGG 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 52
BstSFI CTRYAG 1 cut(s) 713
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 4 cut(s) 384, 483, 563, 584
BstX2I RGATCY 1 cut(s) 678
BstYI RGATCY 1 cut(s) 678
BsuI GTATCC 1 cut(s) 322
BsuRI GGCC 5 cut(s) 51, 137, 215, 318, 581
BtgI CCRYGG 3 cut(s) 132, 382, 582
BtgZI GCGATG 1 cut(s) 286
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 4 cut(s) 33, 90, 339, 679
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 1 cut(s) 531
Cac8I GCNNGC 5 cut(s) 59, 334, 404, 467, 471
CciI TCATGA 1 cut(s) 369
CfoI GCGC 4 cut(s) 445, 483, 553, 563
Cfr10I RCCGGY 2 cut(s) 332, 469
Cfr13I GGNCC 5 cut(s) 50, 213, 214, 261, 379
Cfr42I CCGCGG 2 cut(s) 385, 585
CseI GACGC 1 cut(s) 257
Csp6I GTAC 2 cut(s) 6, 634
CviAII CATG 4 cut(s) 176, 244, 370, 397
CviQI GTAC 2 cut(s) 6, 634
DdeI CTNAG 3 cut(s) 154, 350, 494
DpnI GATC 5 cut(s) 97, 204, 276, 449, 680
DpnII GATC 5 cut(s) 95, 202, 274, 447, 678
DriI GACNNNNNGTC 1 cut(s) 658
EaeI YGGCCR 1 cut(s) 316
Eam1105I GACNNNNNGTC 1 cut(s) 658
EciI GGCGGA 3 cut(s) 88, 316, 628
Ecl136II GAGCTC 3 cut(s) 241, 406, 418
Eco24I GRGCYC 4 cut(s) 217, 243, 408, 420
Eco31I GGTCTC 1 cut(s) 563
Eco47I GGWCC 2 cut(s) 261, 379
Eco53kI GAGCTC 3 cut(s) 241, 406, 418
Eco72I CACGTG 1 cut(s) 631
EcoICRI GAGCTC 3 cut(s) 241, 406, 418
EcoRII CCWGG 1 cut(s) 52
EcoT38I GRGCYC 4 cut(s) 217, 243, 408, 420
FaeI CATG 4 cut(s) 179, 247, 373, 400
FaiI YATR 6 cut(s) 177, 236, 245, 371, 398, 642
FaqI GGGAC 2 cut(s) 247, 392
FatI CATG 4 cut(s) 175, 243, 369, 396
FauI CCCGC 1 cut(s) 239
Fnu4HI GCNGC 7 cut(s) 120, 306, 330, 385, 474, 582, 585
FokI GGATG 4 cut(s) 20, 77, 326, 686
FriOI GRGCYC 4 cut(s) 217, 243, 408, 420
Fsp4HI GCNGC 7 cut(s) 120, 306, 330, 385, 474, 582, 585
GlaI GCGC 4 cut(s) 444, 482, 552, 562
GluI GCNGC 7 cut(s) 120, 306, 330, 385, 474, 582, 585
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 5 cut(s) 51, 137, 215, 318, 581
HapII CCGG 4 cut(s) 259, 315, 333, 470
HgaI GACGC 1 cut(s) 257
HhaI GCGC 4 cut(s) 445, 483, 553, 563
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 4 cut(s) 179, 247, 373, 400
Hin6I GCGC 4 cut(s) 443, 481, 551, 561
HinP1I GCGC 4 cut(s) 443, 481, 551, 561
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 2 cut(s) 86, 227
HpaII CCGG 4 cut(s) 259, 315, 333, 470
HphI GGTGA 3 cut(s) 118, 157, 520
Hpy166II GTNNAC 3 cut(s) 6, 70, 634
Hpy188I TCNGA 6 cut(s) 95, 116, 226, 497, 648, 664
Hpy188III TCNNGA 5 cut(s) 289, 370, 451, 671, 708
Hpy8I GTNNAC 3 cut(s) 6, 70, 634
Hpy99I CGWCG 2 cut(s) 521, 578
HpyAV CCTTC 4 cut(s) 374, 431, 526, 643
HpyCH4III ACNGT 2 cut(s) 574, 714
HpyCH4IV ACGT 3 cut(s) 8, 519, 630
HpyF10VI GCNNNNNNNGC 4 cut(s) 329, 424, 462, 521
HpyF3I CTNAG 3 cut(s) 154, 350, 494
HpySE526I ACGT 3 cut(s) 8, 519, 630
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 4 cut(s) 179, 247, 373, 400
HspAI GCGC 4 cut(s) 443, 481, 551, 561
KroI GCCGGC 2 cut(s) 332, 469
KroNI GCCGGC 2 cut(s) 334, 471
KspI CCGCGG 2 cut(s) 385, 585
Kzo9I GATC 5 cut(s) 95, 202, 274, 447, 678
LmnI GCTCC 2 cut(s) 261, 423
LweI GCATC 1 cut(s) 282
MaeII ACGT 3 cut(s) 8, 519, 630
MaeIII GTNAC 1 cut(s) 124
MalI GATC 5 cut(s) 97, 204, 276, 449, 680
MboI GATC 5 cut(s) 95, 202, 274, 447, 678
MboII GAAGA 2 cut(s) 22, 178
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 5 cut(s) 63, 217, 243, 408, 420
MluCI AATT 2 cut(s) 182, 589
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 1 cut(s) 216
MreI CGCCGGCG 1 cut(s) 469
MroNI GCCGGC 2 cut(s) 332, 469
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 2 cut(s) 384, 584
MspI CCGG 4 cut(s) 259, 315, 333, 470
MspR9I CCNGG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 54
MvnI CGCG 4 cut(s) 384, 483, 563, 584
MwoI GCNNNNNNNGC 4 cut(s) 329, 424, 462, 521
NaeI GCCGGC 2 cut(s) 334, 471
NdeII GATC 5 cut(s) 95, 202, 274, 447, 678
NgoMIV GCCGGC 2 cut(s) 332, 469
NlaIII CATG 4 cut(s) 179, 247, 373, 400
NlaIV GGNNCC 3 cut(s) 215, 263, 380
NmeAIII GCCGAG 2 cut(s) 457, 527
NmuCI GTSAC 1 cut(s) 124
PagI TCATGA 1 cut(s) 369
PdiI GCCGGC 2 cut(s) 334, 471
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 7 cut(s) 121, 307, 331, 386, 475, 583, 586
PleI GAGTC 1 cut(s) 80
PmaCI CACGTG 1 cut(s) 631
PmlI CACGTG 1 cut(s) 631
PpsI GAGTC 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 631
Psp124BI GAGCTC 3 cut(s) 243, 408, 420
Psp6I CCWGG 1 cut(s) 52
PspCI CACGTG 1 cut(s) 631
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 3 cut(s) 215, 263, 380
PspOMI GGGCCC 1 cut(s) 213
PspPI GGNCC 5 cut(s) 50, 213, 214, 261, 379
PsuI RGATCY 1 cut(s) 678
RsaI GTAC 2 cut(s) 7, 635
RsaNI GTAC 2 cut(s) 6, 634
RseI CAYNNNNRTG 1 cut(s) 395
SacI GAGCTC 3 cut(s) 243, 408, 420
SacII CCGCGG 2 cut(s) 385, 585
SatI GCNGC 7 cut(s) 120, 306, 330, 385, 474, 582, 585
Sau3AI GATC 5 cut(s) 95, 202, 274, 447, 678
Sau96I GGNCC 5 cut(s) 50, 213, 214, 261, 379
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 5 cut(s) 63, 217, 243, 408, 420
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 1 cut(s) 713
Sfr303I CCGCGG 2 cut(s) 385, 585
SgrAI CRCCGGYG 1 cut(s) 469
SgrBI CCGCGG 2 cut(s) 385, 585
SinI GGWCC 2 cut(s) 261, 379
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 2 cut(s) 182, 589
SstI GAGCTC 3 cut(s) 243, 408, 420
StyD4I CCNGG 1 cut(s) 52
TaaI ACNGT 2 cut(s) 574, 714
TaiI ACGT 3 cut(s) 11, 522, 633
TaqI TCGA 5 cut(s) 84, 408, 414, 450, 576
TasI AATT 2 cut(s) 182, 589
TauI GCSGC 7 cut(s) 122, 308, 332, 387, 476, 584, 587
TfiI GAWTC 1 cut(s) 227
TscAI CASTG 1 cut(s) 531
TseFI GTSAC 1 cut(s) 124
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 3 cut(s) 22, 328, 379
TspGWI ACGGA 1 cut(s) 639
TspRI CASTG 1 cut(s) 531
VpaK11BI GGWCC 2 cut(s) 261, 379
XapI RAATTY 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.