Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008739

Physical Location & Seq

Genomic Coordinates Reverse (-)

27378747 .. 27379463

Gene Structure 717 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1194300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 717 bp

ATGTGCACGTTAGAGAGGAAAGGCAACATCTTCATCCTAACGCTAACAGGCCTGGGCGAGCACAGGCTCAACCCCACTCTCATCGACTCAATCCGATCCGCTCTCAACCAAATCCGAGCCGCCGTCACCACCACCGCCACGTCATCATCGCCCTCGGCCCTAATCACCACCGCACATGGCAAATTCTTCTCCAACGGCTACGATCTCTCCTGGGCCCAGTCCTCTCAATCCCGCATGGAGCTCATGGACTCCAAGCTCCAGTCCCTTGTCGCCGACCTCATCTCCCTCCCGATGCCCACCATCGCCGCCGTCTCCGGCCACGCCTCCGCCGCCGGCTTCATCCTCGCTCTCTGCCACGACTACCTCCTCATGAGGCGGGACCGCGGCTTCATCTACATGAGCGAGCTCGACATCGAGCTCCTTCTGCCGCGTTGGTTCTTGGCACTTATCGAGAGCAAGGTCGGCTCGCCGGCGGCTCGGCGCGACTTGTTGCTCAGAGCCGATAGGGTGAAAGCCGACGTGGCAGTGGCGAAGGGGATTATCGACTCGGCGCACGATAGCGCGGAGGAAACCGTTGAGGCCGCGGTTAGACTTGGGGAGGAGTTGGTCGCGCGGAAATGGAACGGACACGTGTACGCTCAGATTCGCAAGGACTTGTTGTCTGATGTTCTGGATGAGATCCGCGCGAATAATACTGGCAGTGGATCCCGGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ECH_1

Protein Analysis

239

Amino Acids

25.99

Weight (kDa)

6.6

Isoelectric Point (pI)

52.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	38 - 206	4.8e-20	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008739)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65520
fragaria_vesca	FvH4_7g21971
malus_domestica	MD01G1124100.v1.1 MD01G1124200.v1.1 MD07G1194300.v1.1
prunus_persica	Prupe.2G231700_v2.0.a1
pyrus_communis	pycom01g15060 pycom01g15070 pycom07g18310
rosa_chinensis	RchiOBHm_Chr1g0366311 RchiOBHm_Chr1g0366321
rosa_laevigata	RLG00000027352 RLG00000027353
rosa_multiflora	Rmu_ssc0000250.1_g000002 Rmu_ssc0000250.1_g000004
rosa_roxburghii	Rroxscaffold_4G00290000
rosa_rugosa	Rorug01G0328900
rosa_samantha	Rh1AG337000 Rh1AG337100 Rh1BG298600 Rh1CG313900 Rh1DG330100
rosa_wichuraiana	Rw1G029880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	101
AccII	CGCG	9 cut(s)	384, 430, 483, 563, 584, 611, 613, 684, 686
AclWI	GGATC	4 cut(s)	90, 673, 699, 712
AcoI	YGGCCR	1 cut(s)	316
AcsI	RAATTY	1 cut(s)	182
AcvI	CACGTG	1 cut(s)	631
AfaI	GTAC	1 cut(s)	635
AflIII	ACRYGT	2 cut(s)	628, 630
AhdI	GACNNNNNGTC	1 cut(s)	658
AjiI	CACGTC	2 cut(s)	141, 520
AjnI	CCWGG	2 cut(s)	51, 209
AluBI	AGCT	4 cut(s)	241, 256, 406, 418
AluI	AGCT	4 cut(s)	241, 256, 406, 418
Alw21I	GWGCWC	5 cut(s)	8, 63, 243, 408, 420
Alw26I	GTCTC	1 cut(s)	316
Alw44I	GTGCAC	1 cut(s)	4
AlwI	GGATC	4 cut(s)	90, 673, 699, 712
AoxI	GGCC	5 cut(s)	49, 156, 213, 316, 579
ApaI	GGGCCC	1 cut(s)	217
ApaLI	GTGCAC	1 cut(s)	4
ApoI	RAATTY	1 cut(s)	182
AspLEI	GCGC	5 cut(s)	483, 553, 563, 613, 686
AspS9I	GGNCC	4 cut(s)	157, 213, 214, 379
AsuC2I	CCSGG	1 cut(s)	709
AsuHPI	GGTGA	3 cut(s)	118, 157, 520
AvaII	GGWCC	1 cut(s)	379
BaeGI	GKGCMC	2 cut(s)	8, 217
BamHI	GGATCC	1 cut(s)	704
BanII	GRGCYC	4 cut(s)	217, 243, 408, 420
BbrPI	CACGTG	1 cut(s)	631
Bbv12I	GWGCWC	5 cut(s)	8, 63, 243, 408, 420
BccI	CCATC	1 cut(s)	308
BceAI	ACGGC	3 cut(s)	107, 211, 293
BciT130I	CCWGG	2 cut(s)	53, 211
BcnI	CCSGG	1 cut(s)	709
BcoDI	GTCTC	1 cut(s)	316
BfmI	CTRYAG	1 cut(s)	713
BglI	GCCNNNNNGGC	1 cut(s)	521
BisI	GCNGC	7 cut(s)	120, 306, 330, 385, 428, 474, 582
BlsI	GCNGC	7 cut(s)	121, 307, 331, 386, 429, 475, 583
Bme1390I	CCNGG	3 cut(s)	53, 211, 709
Bme18I	GGWCC	1 cut(s)	379
BmeRI	GACNNNNNGTC	1 cut(s)	658
BmgBI	CACGTC	2 cut(s)	141, 520
BmgT120I	GGNCC	4 cut(s)	157, 213, 214, 379
BmiI	GGNNCC	3 cut(s)	215, 380, 706
BmrFI	CCNGG	3 cut(s)	53, 211, 709
BmrI	ACTGGG	1 cut(s)	211
BmsI	GCATC	1 cut(s)	282
BmuI	ACTGGG	1 cut(s)	211
BpmI	CTGGAG	1 cut(s)	242
BpuMI	CCSGG	1 cut(s)	709
BsaAI	YACGTR	1 cut(s)	631
BsaJI	CCNNGG	5 cut(s)	52, 153, 210, 382, 582
BsaXI	ACNNNNNCTCC	4 cut(s)	191, 221, 266, 296
Bse118I	RCCGGY	2 cut(s)	332, 469
Bse1I	ACTGG	3 cut(s)	217, 259, 700
BseBI	CCWGG	2 cut(s)	53, 211
BseDI	CCNNGG	5 cut(s)	52, 153, 210, 382, 582
BseGI	GGATG	3 cut(s)	33, 339, 679
BseMII	CTCAG	2 cut(s)	508, 653
BseNI	ACTGG	3 cut(s)	217, 259, 700
BseRI	GAGGAG	2 cut(s)	356, 614
BseSI	GKGCMC	2 cut(s)	8, 217
Bsh1236I	CGCG	9 cut(s)	384, 430, 483, 563, 584, 611, 613, 684, 686
BshFI	GGCC	5 cut(s)	51, 158, 215, 318, 581
BsiHKAI	GWGCWC	5 cut(s)	8, 63, 243, 408, 420
BsiSI	CCGG	4 cut(s)	315, 333, 470, 709
BslFI	GGGAC	2 cut(s)	247, 392
BsmAI	GTCTC	1 cut(s)	316
BsmBI	CGTCTC	1 cut(s)	316
BsmFI	GGGAC	2 cut(s)	247, 392
BsnI	GGCC	5 cut(s)	51, 158, 215, 318, 581
Bsp120I	GGGCCC	1 cut(s)	213
Bsp1286I	GDGCHC	6 cut(s)	8, 63, 217, 243, 408, 420
Bsp143I	GATC	4 cut(s)	95, 202, 678, 704
BspANI	GGCC	5 cut(s)	51, 158, 215, 318, 581
BspCNI	CTCAG	2 cut(s)	507, 652
BspFNI	CGCG	9 cut(s)	384, 430, 483, 563, 584, 611, 613, 684, 686
BspHI	TCATGA	1 cut(s)	369
BspLI	GGNNCC	3 cut(s)	215, 380, 706
BspPI	GGATC	4 cut(s)	90, 673, 699, 712
BsrBI	CCGCTC	1 cut(s)	101
BsrFI	RCCGGY	2 cut(s)	332, 469
BsrI	ACTGG	3 cut(s)	217, 259, 700
BssAI	RCCGGY	2 cut(s)	332, 469
BssECI	CCNNGG	5 cut(s)	52, 153, 210, 382, 582
BssMI	GATC	4 cut(s)	95, 202, 678, 704
Bst2UI	CCWGG	2 cut(s)	53, 211
Bst4CI	ACNGT	1 cut(s)	574
BstBAI	YACGTR	1 cut(s)	631
BstC8I	GCNNGC	5 cut(s)	59, 334, 404, 467, 471
BstDEI	CTNAG	2 cut(s)	494, 639
BstDSI	CCRYGG	2 cut(s)	382, 582
BstF5I	GGATG	3 cut(s)	33, 339, 679
BstFNI	CGCG	9 cut(s)	384, 430, 483, 563, 584, 611, 613, 684, 686
BstHHI	GCGC	5 cut(s)	483, 553, 563, 613, 686
BstKTI	GATC	4 cut(s)	98, 205, 681, 707
BstMAI	GTCTC	1 cut(s)	316
BstMBI	GATC	4 cut(s)	95, 202, 678, 704
BstMWI	GCNNNNNNNGC	4 cut(s)	329, 424, 462, 521
BstNI	CCWGG	2 cut(s)	53, 211
BstSCI	CCNGG	3 cut(s)	51, 209, 707
BstSFI	CTRYAG	1 cut(s)	713
BstSLI	GKGCMC	2 cut(s)	8, 217
BstUI	CGCG	9 cut(s)	384, 430, 483, 563, 584, 611, 613, 684, 686
BstX2I	RGATCY	2 cut(s)	678, 704
BstYI	RGATCY	2 cut(s)	678, 704
BsuRI	GGCC	5 cut(s)	51, 158, 215, 318, 581
BtgI	CCRYGG	2 cut(s)	382, 582
BtgZI	GCGATG	2 cut(s)	132, 286
BtrI	CACGTC	2 cut(s)	141, 520
BtsCI	GGATG	3 cut(s)	33, 339, 679
BtsI	GCAGTG	2 cut(s)	531, 706
BtsIMutI	CAGTG	2 cut(s)	531, 706
Cac8I	GCNNGC	5 cut(s)	59, 334, 404, 467, 471
CciI	TCATGA	1 cut(s)	369
CfoI	GCGC	5 cut(s)	483, 553, 563, 613, 686
Cfr10I	RCCGGY	2 cut(s)	332, 469
Cfr13I	GGNCC	4 cut(s)	157, 213, 214, 379
Cfr42I	CCGCGG	2 cut(s)	385, 585
Csp6I	GTAC	1 cut(s)	634
CviAII	CATG	5 cut(s)	176, 235, 244, 370, 397
CviQI	GTAC	1 cut(s)	634
DdeI	CTNAG	2 cut(s)	494, 639
DpnI	GATC	4 cut(s)	97, 204, 680, 706
DpnII	GATC	4 cut(s)	95, 202, 678, 704
DriI	GACNNNNNGTC	1 cut(s)	658
EaeI	YGGCCR	1 cut(s)	316
Eam1105I	GACNNNNNGTC	1 cut(s)	658
EciI	GGCGGA	1 cut(s)	316
Ecl136II	GAGCTC	3 cut(s)	241, 406, 418
Eco147I	AGGCCT	1 cut(s)	51
Eco24I	GRGCYC	4 cut(s)	217, 243, 408, 420
Eco47I	GGWCC	1 cut(s)	379
Eco53kI	GAGCTC	3 cut(s)	241, 406, 418
Eco72I	CACGTG	1 cut(s)	631
EcoICRI	GAGCTC	3 cut(s)	241, 406, 418
EcoRII	CCWGG	2 cut(s)	51, 209
EcoT38I	GRGCYC	4 cut(s)	217, 243, 408, 420
Esp3I	CGTCTC	1 cut(s)	316
FaeI	CATG	5 cut(s)	179, 238, 247, 373, 400
FaiI	YATR	5 cut(s)	177, 236, 245, 371, 398
FaqI	GGGAC	2 cut(s)	247, 392
FatI	CATG	5 cut(s)	175, 234, 243, 369, 396
FauI	CCCGC	2 cut(s)	239, 369
Fnu4HI	GCNGC	7 cut(s)	120, 306, 330, 385, 428, 474, 582
FokI	GGATG	3 cut(s)	20, 326, 686
FriOI	GRGCYC	4 cut(s)	217, 243, 408, 420
Fsp4HI	GCNGC	7 cut(s)	120, 306, 330, 385, 428, 474, 582
GlaI	GCGC	5 cut(s)	482, 552, 562, 612, 685
GluI	GCNGC	7 cut(s)	120, 306, 330, 385, 428, 474, 582
GsuI	CTGGAG	1 cut(s)	242
HaeIII	GGCC	5 cut(s)	51, 158, 215, 318, 581
HapII	CCGG	4 cut(s)	315, 333, 470, 709
HhaI	GCGC	5 cut(s)	483, 553, 563, 613, 686
Hin1II	CATG	5 cut(s)	179, 238, 247, 373, 400
Hin6I	GCGC	5 cut(s)	481, 551, 561, 611, 684
HinP1I	GCGC	5 cut(s)	481, 551, 561, 611, 684
HinfI	GANTC	4 cut(s)	86, 248, 545, 643
HpaII	CCGG	4 cut(s)	315, 333, 470, 709
HphI	GGTGA	3 cut(s)	118, 157, 520
Hpy166II	GTNNAC	2 cut(s)	6, 634
Hpy188I	TCNGA	5 cut(s)	95, 116, 497, 642, 664
Hpy188III	TCNNGA	4 cut(s)	289, 370, 451, 671
Hpy8I	GTNNAC	2 cut(s)	6, 634
Hpy99I	CGWCG	1 cut(s)	521
HpyAV	CCTTC	2 cut(s)	431, 526
HpyCH4III	ACNGT	1 cut(s)	574
HpyCH4IV	ACGT	4 cut(s)	8, 140, 519, 630
HpyCH4V	TGCA	1 cut(s)	6
HpyF10VI	GCNNNNNNNGC	4 cut(s)	329, 424, 462, 521
HpyF3I	CTNAG	2 cut(s)	494, 639
HpySE526I	ACGT	4 cut(s)	8, 140, 519, 630
Hsp92II	CATG	5 cut(s)	179, 238, 247, 373, 400
HspAI	GCGC	5 cut(s)	481, 551, 561, 611, 684
KroI	GCCGGC	2 cut(s)	332, 469
KroNI	GCCGGC	2 cut(s)	334, 471
KspI	CCGCGG	2 cut(s)	385, 585
Kzo9I	GATC	4 cut(s)	95, 202, 678, 704
LmnI	GCTCC	3 cut(s)	238, 261, 423
LweI	GCATC	1 cut(s)	282
MaeII	ACGT	4 cut(s)	8, 140, 519, 630
MaeIII	GTNAC	1 cut(s)	124
MalI	GATC	4 cut(s)	97, 204, 680, 706
MbiI	CCGCTC	1 cut(s)	101
MboI	GATC	4 cut(s)	95, 202, 678, 704
MboII	GAAGA	2 cut(s)	22, 178
MflI	RGATCY	2 cut(s)	678, 704
MhlI	GDGCHC	6 cut(s)	8, 63, 217, 243, 408, 420
MluCI	AATT	1 cut(s)	182
MlyI	GAGTC	3 cut(s)	80, 242, 539
MmeI	TCCRAC	1 cut(s)	216
MreI	CGCCGGCG	1 cut(s)	469
MroNI	GCCGGC	2 cut(s)	332, 469
MslI	CAYNNNNRTG	1 cut(s)	395
MspA1I	CMGCKG	2 cut(s)	384, 584
MspI	CCGG	4 cut(s)	315, 333, 470, 709
MspR9I	CCNGG	3 cut(s)	53, 211, 709
MvaI	CCWGG	2 cut(s)	53, 211
MvnI	CGCG	9 cut(s)	384, 430, 483, 563, 584, 611, 613, 684, 686
MwoI	GCNNNNNNNGC	4 cut(s)	329, 424, 462, 521
NaeI	GCCGGC	2 cut(s)	334, 471
NciI	CCSGG	1 cut(s)	709
NdeII	GATC	4 cut(s)	95, 202, 678, 704
NgoMIV	GCCGGC	2 cut(s)	332, 469
NlaIII	CATG	5 cut(s)	179, 238, 247, 373, 400
NlaIV	GGNNCC	3 cut(s)	215, 380, 706
NmeAIII	GCCGAG	3 cut(s)	134, 457, 527
NmuCI	GTSAC	1 cut(s)	124
PagI	TCATGA	1 cut(s)	369
PceI	AGGCCT	1 cut(s)	51
PdiI	GCCGGC	2 cut(s)	334, 471
PfeI	GAWTC	1 cut(s)	643
PkrI	GCNGC	7 cut(s)	121, 307, 331, 386, 429, 475, 583
PleI	GAGTC	3 cut(s)	80, 242, 539
PmaCI	CACGTG	1 cut(s)	631
PmlI	CACGTG	1 cut(s)	631
PpsI	GAGTC	3 cut(s)	80, 242, 539
Ppu21I	YACGTR	1 cut(s)	631
Psp124BI	GAGCTC	3 cut(s)	243, 408, 420
Psp6I	CCWGG	2 cut(s)	51, 209
PspCI	CACGTG	1 cut(s)	631
PspGI	CCWGG	2 cut(s)	51, 209
PspN4I	GGNNCC	3 cut(s)	215, 380, 706
PspOMI	GGGCCC	1 cut(s)	213
PspPI	GGNCC	4 cut(s)	157, 213, 214, 379
PsuI	RGATCY	2 cut(s)	678, 704
RsaI	GTAC	1 cut(s)	635
RsaNI	GTAC	1 cut(s)	634
RseI	CAYNNNNRTG	1 cut(s)	395
SacI	GAGCTC	3 cut(s)	243, 408, 420
SacII	CCGCGG	2 cut(s)	385, 585
SatI	GCNGC	7 cut(s)	120, 306, 330, 385, 428, 474, 582
Sau3AI	GATC	4 cut(s)	95, 202, 678, 704
Sau96I	GGNCC	4 cut(s)	157, 213, 214, 379
SchI	GAGTC	3 cut(s)	80, 242, 539
ScrFI	CCNGG	3 cut(s)	53, 211, 709
SduI	GDGCHC	6 cut(s)	8, 63, 217, 243, 408, 420
SfaNI	GCATC	1 cut(s)	282
SfcI	CTRYAG	1 cut(s)	713
Sfr303I	CCGCGG	2 cut(s)	385, 585
SgrAI	CRCCGGYG	1 cut(s)	469
SgrBI	CCGCGG	2 cut(s)	385, 585
SinI	GGWCC	1 cut(s)	379
SmiMI	CAYNNNNRTG	1 cut(s)	395
Sse9I	AATT	1 cut(s)	182
SseBI	AGGCCT	1 cut(s)	51
SstI	GAGCTC	3 cut(s)	243, 408, 420
StuI	AGGCCT	1 cut(s)	51
StyD4I	CCNGG	3 cut(s)	51, 209, 707
TaaI	ACNGT	1 cut(s)	574
TaiI	ACGT	4 cut(s)	11, 143, 522, 633
TaqI	TCGA	5 cut(s)	84, 408, 414, 450, 543
TasI	AATT	1 cut(s)	182
TauI	GCSGC	7 cut(s)	122, 308, 332, 387, 430, 476, 584
TfiI	GAWTC	1 cut(s)	643
TscAI	CASTG	2 cut(s)	531, 706
TseFI	GTSAC	1 cut(s)	124
Tsp45I	GTSAC	1 cut(s)	124
TspDTI	ATGAA	3 cut(s)	22, 328, 379
TspGWI	ACGGA	1 cut(s)	639
TspRI	CASTG	2 cut(s)	531, 706
VneI	GTGCAC	1 cut(s)	4
VpaK11BI	GGWCC	1 cut(s)	379
XapI	RAATTY	1 cut(s)	182

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.