pycom01g15060

Enoyl-CoA hydratase/isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008739
Physical Location & Seq
Reverse (-)
15074671 .. 15075387
717 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g15060.1

Sequence Viewer

Length: 717 bp
ATGTGCACATTAGAGAGGAAAGGCAACGTCTTCATCCTAACGCTAACAGGCCCGGGCGAGCACAGACTCAATCCCACTTTCATCGACTCGATCCGGTCCGCCCTCAACCAAATCCGAACCGCCGTTACAACCACCGCCACGTCATCATCACCCTCAGCCCTAATCACCACCGCACATGGCAAATTCTTCTCCAACGGCTACGATCTCTCCTGGGCCAAGTCCTCCGAGTCCCGGTTGGAGCTCATGCGCTCCAAGGTCCGGTCCCTCGTCGCCGACCTTATCTCCCTCCCCATGCCCACCATCGCCGCCGTTTCCGGCCACGCTTCCGCCGCCGGCTTCATCCTCGCTCGCAGCCACGACTACCTCCTCATGAGGCGAGACCGCGGCTTCATCTACATGAGCGAGCTCGACATCGAGCTCCTTCTCCCGGCTTGGTTAGCGGCGCTGTTCGATCGCAAAATCAGCTCACCGGCGGCTCGGCGCGATTTGTTGCTGAGAGCCGATAAGATGACGGCGGACGTGGCAGTGGCGAACGGGATAATCGACTCGGCGCACGATAGCGCGGAGGAGACGGTTGAGGCCGCGGTTAGACTTGGAGAGGAGTTGGTCTCGCGGAAATGGAACGGACACGTGTACGCTCAGATTCGCAAGGACTTGTTGTCTGAAGTACTGGATGAGATCCGCGTGTATAATACTGGCAGTAGAGCGCGGCTGTAG

Protein Analysis

239

Amino Acids

26.21

Weight (kDa)

8.73

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 6 - 206 2e-19 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 7 cut(s) 384, 483, 563, 584, 613, 684, 709
AclWI GGATC 2 cut(s) 85, 673
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 684
AcvI CACGTG 1 cut(s) 631
AfaI GTAC 2 cut(s) 635, 669
AfiI CCNNNNNNNGG 3 cut(s) 231, 258, 427
AflIII ACRYGT 2 cut(s) 628, 630
AhdI GACNNNNNGTC 1 cut(s) 658
AjiI CACGTC 2 cut(s) 141, 520
AjnI CCWGG 1 cut(s) 209
AluBI AGCT 4 cut(s) 241, 406, 418, 465
AluI AGCT 4 cut(s) 241, 406, 418, 465
Alw21I GWGCWC 5 cut(s) 8, 63, 243, 408, 420
Alw26I GTCTC 3 cut(s) 372, 563, 613
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 2 cut(s) 85, 673
Ama87I CYCGRG 1 cut(s) 52
AoxI GGCC 4 cut(s) 49, 213, 316, 579
ApaLI GTGCAC 1 cut(s) 4
ApeKI GCWGC 1 cut(s) 351
ApoI RAATTY 1 cut(s) 182
AspLEI GCGC 6 cut(s) 249, 445, 483, 553, 563, 709
AspS9I GGNCC 5 cut(s) 50, 96, 213, 256, 261
AsuC2I CCSGG 4 cut(s) 53, 54, 232, 428
AsuHPI GGTGA 3 cut(s) 141, 157, 459
AvaI CYCGRG 1 cut(s) 52
AvaII GGWCC 3 cut(s) 96, 256, 261
BaeGI GKGCMC 1 cut(s) 8
BanII GRGCYC 3 cut(s) 243, 408, 420
BbrPI CACGTG 1 cut(s) 631
BbsI GAAGAC 1 cut(s) 22
Bbv12I GWGCWC 5 cut(s) 8, 63, 243, 408, 420
BbvCI CCTCAGC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 363
BccI CCATC 1 cut(s) 308
BceAI ACGGC 4 cut(s) 107, 211, 293, 528
BciT130I CCWGG 1 cut(s) 211
BcnI CCSGG 4 cut(s) 53, 54, 232, 428
BcoDI GTCTC 3 cut(s) 372, 563, 613
BfmI CTRYAG 1 cut(s) 713
BfoI RGCGCY 1 cut(s) 446
BisI GCNGC 8 cut(s) 306, 330, 352, 385, 441, 474, 582, 710
BlsI GCNGC 8 cut(s) 307, 331, 353, 386, 442, 475, 583, 711
BmcAI AGTACT 1 cut(s) 669
Bme1390I CCNGG 5 cut(s) 53, 54, 211, 232, 428
Bme18I GGWCC 3 cut(s) 96, 256, 261
BmeRI GACNNNNNGTC 1 cut(s) 658
BmeT110I CYCGRG 1 cut(s) 52
BmgBI CACGTC 2 cut(s) 141, 520
BmgT120I GGNCC 5 cut(s) 50, 96, 213, 256, 261
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 5 cut(s) 53, 54, 211, 232, 428
BpiI GAAGAC 1 cut(s) 22
BplI GAGNNNNNCTC 2 cut(s) 593, 625
Bpu10I CCTNAGC 1 cut(s) 154
BpuMI CCSGG 4 cut(s) 53, 54, 232, 428
BsaAI YACGTR 1 cut(s) 631
BsaI GGTCTC 2 cut(s) 372, 613
BsaJI CCNNGG 5 cut(s) 52, 210, 252, 382, 582
BsaWI WCCGGW 2 cut(s) 93, 258
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 266, 296
Bsc4I CCNNNNNNNGG 3 cut(s) 231, 258, 427
Bse118I RCCGGY 2 cut(s) 332, 469
Bse1I ACTGG 2 cut(s) 675, 700
BseBI CCWGG 1 cut(s) 211
BseDI CCNNGG 5 cut(s) 52, 210, 252, 382, 582
BseGI GGATG 3 cut(s) 33, 339, 679
BseLI CCNNNNNNNGG 3 cut(s) 231, 258, 427
BseMII CTCAG 3 cut(s) 168, 485, 653
BseNI ACTGG 2 cut(s) 675, 700
BseRI GAGGAG 3 cut(s) 356, 581, 614
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 1 cut(s) 363
Bsh1236I CGCG 7 cut(s) 384, 483, 563, 584, 613, 684, 709
Bsh1285I CGRYCG 1 cut(s) 454
BshFI GGCC 4 cut(s) 51, 215, 318, 581
BsiEI CGRYCG 1 cut(s) 454
BsiHKAI GWGCWC 5 cut(s) 8, 63, 243, 408, 420
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 8 cut(s) 53, 94, 232, 259, 315, 333, 428, 470
BslFI GGGAC 2 cut(s) 214, 247
BslI CCNNNNNNNGG 3 cut(s) 231, 258, 427
BsmAI GTCTC 3 cut(s) 372, 563, 613
BsmBI CGTCTC 1 cut(s) 563
BsmFI GGGAC 2 cut(s) 214, 247
BsnI GGCC 4 cut(s) 51, 215, 318, 581
Bso31I GGTCTC 2 cut(s) 372, 613
BsoBI CYCGRG 1 cut(s) 52
Bsp1286I GDGCHC 5 cut(s) 8, 63, 243, 408, 420
Bsp143I GATC 4 cut(s) 90, 202, 451, 678
BspANI GGCC 4 cut(s) 51, 215, 318, 581
BspCNI CTCAG 3 cut(s) 167, 486, 652
BspFNI CGCG 7 cut(s) 384, 483, 563, 584, 613, 684, 709
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 263
BspPI GGATC 2 cut(s) 85, 673
BspTNI GGTCTC 2 cut(s) 372, 613
BsrFI RCCGGY 2 cut(s) 332, 469
BsrI ACTGG 2 cut(s) 675, 700
BssAI RCCGGY 2 cut(s) 332, 469
BssECI CCNNGG 5 cut(s) 52, 210, 252, 382, 582
BssMI GATC 4 cut(s) 90, 202, 451, 678
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 211
Bst4CI ACNGT 1 cut(s) 574
BstBAI YACGTR 1 cut(s) 631
BstC8I GCNNGC 4 cut(s) 59, 334, 349, 404
BstDEI CTNAG 3 cut(s) 154, 494, 639
BstDSI CCRYGG 2 cut(s) 382, 582
BstF5I GGATG 3 cut(s) 33, 339, 679
BstFNI CGCG 7 cut(s) 384, 483, 563, 584, 613, 684, 709
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 6 cut(s) 249, 445, 483, 553, 563, 709
BstKTI GATC 4 cut(s) 93, 205, 454, 681
BstMAI GTCTC 3 cut(s) 372, 563, 613
BstMBI GATC 4 cut(s) 90, 202, 451, 678
BstMCI CGRYCG 1 cut(s) 454
BstMWI GCNNNNNNNGC 4 cut(s) 329, 437, 462, 521
BstNI CCWGG 1 cut(s) 211
BstSCI CCNGG 5 cut(s) 51, 52, 209, 230, 426
BstSFI CTRYAG 1 cut(s) 713
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 7 cut(s) 384, 483, 563, 584, 613, 684, 709
BstV1I GCAGC 1 cut(s) 363
BstV2I GAAGAC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 678
BstYI RGATCY 1 cut(s) 678
BsuRI GGCC 4 cut(s) 51, 215, 318, 581
BtgI CCRYGG 2 cut(s) 382, 582
BtgZI GCGATG 1 cut(s) 286
BtrI CACGTC 2 cut(s) 141, 520
BtsCI GGATG 3 cut(s) 33, 339, 679
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 1 cut(s) 531
Cac8I GCNNGC 4 cut(s) 59, 334, 349, 404
CciI TCATGA 1 cut(s) 369
CfoI GCGC 6 cut(s) 249, 445, 483, 553, 563, 709
Cfr10I RCCGGY 2 cut(s) 332, 469
Cfr13I GGNCC 5 cut(s) 50, 96, 213, 256, 261
Cfr42I CCGCGG 2 cut(s) 385, 585
Cfr9I CCCGGG 1 cut(s) 52
CpoI CGGWCCG 1 cut(s) 96
Csp6I GTAC 2 cut(s) 634, 668
CspI CGGWCCG 1 cut(s) 96
CviAII CATG 5 cut(s) 176, 244, 292, 370, 397
CviQI GTAC 2 cut(s) 634, 668
DdeI CTNAG 3 cut(s) 154, 494, 639
DpnI GATC 4 cut(s) 92, 204, 453, 680
DpnII GATC 4 cut(s) 90, 202, 451, 678
DriI GACNNNNNGTC 1 cut(s) 658
EaeI YGGCCR 1 cut(s) 316
Eam1105I GACNNNNNGTC 1 cut(s) 658
EciI GGCGGA 3 cut(s) 88, 316, 530
Ecl136II GAGCTC 3 cut(s) 241, 406, 418
Eco130I CCWWGG 1 cut(s) 252
Eco24I GRGCYC 3 cut(s) 243, 408, 420
Eco31I GGTCTC 2 cut(s) 372, 613
Eco47I GGWCC 3 cut(s) 96, 256, 261
Eco53kI GAGCTC 3 cut(s) 241, 406, 418
Eco57I CTGAAG 1 cut(s) 684
Eco72I CACGTG 1 cut(s) 631
Eco88I CYCGRG 1 cut(s) 52
EcoICRI GAGCTC 3 cut(s) 241, 406, 418
EcoRII CCWGG 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 252
EcoT38I GRGCYC 3 cut(s) 243, 408, 420
ErhI CCWWGG 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 563
FaeI CATG 5 cut(s) 179, 247, 295, 373, 400
FaiI YATR 6 cut(s) 177, 245, 293, 371, 398, 690
FaqI GGGAC 2 cut(s) 214, 247
FatI CATG 5 cut(s) 175, 243, 291, 369, 396
Fnu4HI GCNGC 8 cut(s) 306, 330, 352, 385, 441, 474, 582, 710
FokI GGATG 3 cut(s) 20, 326, 686
FriOI GRGCYC 3 cut(s) 243, 408, 420
Fsp4HI GCNGC 8 cut(s) 306, 330, 352, 385, 441, 474, 582, 710
GlaI GCGC 6 cut(s) 248, 444, 482, 552, 562, 708
GluI GCNGC 8 cut(s) 306, 330, 352, 385, 441, 474, 582, 710
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 4 cut(s) 51, 215, 318, 581
HapII CCGG 8 cut(s) 53, 94, 232, 259, 315, 333, 428, 470
HhaI GCGC 6 cut(s) 249, 445, 483, 553, 563, 709
Hin1II CATG 5 cut(s) 179, 247, 295, 373, 400
Hin6I GCGC 6 cut(s) 247, 443, 481, 551, 561, 707
HinP1I GCGC 6 cut(s) 247, 443, 481, 551, 561, 707
HinfI GANTC 5 cut(s) 66, 86, 227, 545, 643
HpaII CCGG 8 cut(s) 53, 94, 232, 259, 315, 333, 428, 470
HphI GGTGA 3 cut(s) 141, 157, 459
Hpy166II GTNNAC 2 cut(s) 6, 634
Hpy188I TCNGA 4 cut(s) 116, 226, 642, 664
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 2 cut(s) 6, 634
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 1 cut(s) 574
HpyCH4IV ACGT 4 cut(s) 27, 140, 519, 630
HpyCH4V TGCA 1 cut(s) 6
HpyF10VI GCNNNNNNNGC 4 cut(s) 329, 437, 462, 521
HpyF3I CTNAG 3 cut(s) 154, 494, 639
HpySE526I ACGT 4 cut(s) 27, 140, 519, 630
Hsp92II CATG 5 cut(s) 179, 247, 295, 373, 400
HspAI GCGC 6 cut(s) 247, 443, 481, 551, 561, 707
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
KspI CCGCGG 2 cut(s) 385, 585
Kzo9I GATC 4 cut(s) 90, 202, 451, 678
LmnI GCTCC 3 cut(s) 238, 254, 423
Lsp1109I GCAGC 1 cut(s) 363
MaeII ACGT 4 cut(s) 27, 140, 519, 630
MaeIII GTNAC 1 cut(s) 124
MalI GATC 4 cut(s) 92, 204, 453, 680
MboI GATC 4 cut(s) 90, 202, 451, 678
MboII GAAGA 2 cut(s) 22, 178
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 5 cut(s) 8, 63, 243, 408, 420
MluCI AATT 1 cut(s) 182
MlyI GAGTC 4 cut(s) 60, 80, 236, 539
MmeI TCCRAC 2 cut(s) 216, 216
MroNI GCCGGC 1 cut(s) 332
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 2 cut(s) 384, 584
MspI CCGG 8 cut(s) 53, 94, 232, 259, 315, 333, 428, 470
MspR9I CCNGG 5 cut(s) 53, 54, 211, 232, 428
MvaI CCWGG 1 cut(s) 211
MvnI CGCG 7 cut(s) 384, 483, 563, 584, 613, 684, 709
MwoI GCNNNNNNNGC 4 cut(s) 329, 437, 462, 521
NaeI GCCGGC 1 cut(s) 334
NciI CCSGG 4 cut(s) 53, 54, 232, 428
NdeII GATC 4 cut(s) 90, 202, 451, 678
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 5 cut(s) 179, 247, 295, 373, 400
NlaIV GGNNCC 1 cut(s) 263
NmeAIII GCCGAG 2 cut(s) 457, 527
PagI TCATGA 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PdiI GCCGGC 1 cut(s) 334
PfeI GAWTC 1 cut(s) 643
PkrI GCNGC 8 cut(s) 307, 331, 353, 386, 442, 475, 583, 711
Ple19I CGATCG 1 cut(s) 454
PleI GAGTC 4 cut(s) 60, 80, 235, 539
PmaCI CACGTG 1 cut(s) 631
PmlI CACGTG 1 cut(s) 631
PpsI GAGTC 4 cut(s) 60, 80, 235, 539
Ppu21I YACGTR 1 cut(s) 631
Psp124BI GAGCTC 3 cut(s) 243, 408, 420
Psp6I CCWGG 1 cut(s) 209
PspCI CACGTG 1 cut(s) 631
PspGI CCWGG 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 263
PspPI GGNCC 5 cut(s) 50, 96, 213, 256, 261
PsrI GAACNNNNNNTAC 2 cut(s) 109, 141
PsuI RGATCY 1 cut(s) 678
PvuI CGATCG 1 cut(s) 454
RsaI GTAC 2 cut(s) 635, 669
RsaNI GTAC 2 cut(s) 634, 668
RseI CAYNNNNRTG 1 cut(s) 395
Rsr2I CGGWCCG 1 cut(s) 96
RsrII CGGWCCG 1 cut(s) 96
SacI GAGCTC 3 cut(s) 243, 408, 420
SacII CCGCGG 2 cut(s) 385, 585
SatI GCNGC 8 cut(s) 306, 330, 352, 385, 441, 474, 582, 710
Sau3AI GATC 4 cut(s) 90, 202, 451, 678
Sau96I GGNCC 5 cut(s) 50, 96, 213, 256, 261
ScaI AGTACT 1 cut(s) 669
SchI GAGTC 4 cut(s) 60, 80, 236, 539
ScrFI CCNGG 5 cut(s) 53, 54, 211, 232, 428
SduI GDGCHC 5 cut(s) 8, 63, 243, 408, 420
SfcI CTRYAG 1 cut(s) 713
Sfr303I CCGCGG 2 cut(s) 385, 585
SgrAI CRCCGGYG 1 cut(s) 469
SgrBI CCGCGG 2 cut(s) 385, 585
SinI GGWCC 3 cut(s) 96, 256, 261
SmaI CCCGGG 1 cut(s) 54
SmiMI CAYNNNNRTG 1 cut(s) 395
SrfI GCCCGGGC 1 cut(s) 54
Sse9I AATT 1 cut(s) 182
SstI GAGCTC 3 cut(s) 243, 408, 420
StyD4I CCNGG 5 cut(s) 51, 52, 209, 230, 426
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 574
TaiI ACGT 4 cut(s) 30, 143, 522, 633
TaqI TCGA 6 cut(s) 84, 89, 408, 414, 450, 543
TasI AATT 1 cut(s) 182
TatI WGTACW 1 cut(s) 667
TauI GCSGC 7 cut(s) 308, 332, 387, 443, 476, 584, 712
TfiI GAWTC 1 cut(s) 643
TscAI CASTG 1 cut(s) 531
TseI GCWGC 1 cut(s) 351
TspDTI ATGAA 4 cut(s) 22, 70, 328, 379
TspGWI ACGGA 1 cut(s) 639
TspMI CCCGGG 1 cut(s) 52
TspRI CASTG 1 cut(s) 531
VneI GTGCAC 1 cut(s) 4
VpaK11BI GGWCC 3 cut(s) 96, 256, 261
XapI RAATTY 1 cut(s) 182
XmaI CCCGGG 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.