Rw1G029880

Enoyl-CoA hydratase/isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008739
Physical Location & Seq
Reverse (-)
57570283 .. 57576636
6354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029880.1

Sequence Viewer

Length: 729 bp
ATGCATTGTGAAAAGTGTGAGACTAAGAAAATGTGCACGTTGGACAAGAAAGGCGAGGTCTTCATCCTCACCCTAACTGGGCCGGGTGAGCATAGGCTCAACCCAACTCTACTGGATGCAATCCAATCCGCTTTAAACACCGTCCGAGCCGCCGCCACGTCATCCTCCGTAGCCCTGATCACCACCGCACACGGCAAATTCTTCTCCAACGGCTTCGATCTGGACTGGGCCGGATCGGACAAAGCCCGAGGCGAGCTCATGACCTCCAAGCTCCGGTCGGTCGTGGCCAACTTCATCTCCCTCCCGCTGCCGACCATCGTCGCCGTCACCGGCCACGCCTCCGCCGCCGGCTTCATCCTCGCGCGGTGCCACGACTATGTTTTTATGAGGAAGGACAGGGGGTTCGTCTACATGAGCGAGATGGACATCGCGCTGGTGATTCCGGCTTGGGTTCACGCGCTGATTAAGAACAAGGTCGGGTCGGCGGCGGCGCGGCGCGATTTGTTGCTCAGAGCGGATAAGATGACGGCGGCGGTGGCCGTGGAGAAGGGGATTGTTGACTCGGCGGTTAATGGTGCGGAGGAGACGGTTGAGGCGGCGGTTAGATTGGGGGAGGAGTTGGTTAGGAGGAAGTGGAAGGGACACGTGTACGCTCAGATTCGCCTTGGCTTGATGTCGGAGGTCTTGGAGGCGATGCGAAACCATGACAGTACTAGATCCCTACTGTAG

Protein Analysis

242

Amino Acids

26.38

Weight (kDa)

9.06

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 17 - 210 9e-22 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 366
AccBSI CCGCTC 1 cut(s) 515
AccI GTMKAC 1 cut(s) 408
AccII CGCG 6 cut(s) 362, 364, 431, 458, 493, 498
AclWI GGATC 2 cut(s) 241, 711
AcoI YGGCCR 3 cut(s) 285, 331, 537
AcsI RAATTY 1 cut(s) 197
AcvI CACGTG 1 cut(s) 646
AfaI GTAC 2 cut(s) 650, 712
AfiI CCNNNNNNNGG 2 cut(s) 78, 273
AflIII ACRYGT 2 cut(s) 643, 645
AjiI CACGTC 1 cut(s) 159
AloI GAACNNNNNNTCC 2 cut(s) 386, 418
AluBI AGCT 2 cut(s) 256, 271
AluI AGCT 2 cut(s) 256, 271
Alw21I GWGCWC 2 cut(s) 38, 258
Alw26I GTCTC 2 cut(s) 14, 578
Alw44I GTGCAC 1 cut(s) 34
AlwI GGATC 2 cut(s) 241, 711
Ama87I CYCGRG 1 cut(s) 246
AoxI GGCC 5 cut(s) 80, 228, 285, 331, 537
ApaLI GTGCAC 1 cut(s) 34
ApeKI GCWGC 1 cut(s) 307
ApoI RAATTY 1 cut(s) 197
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
AspLEI GCGC 5 cut(s) 364, 433, 460, 493, 498
AspS9I GGNCC 2 cut(s) 80, 228
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 5 cut(s) 61, 98, 172, 319, 448
AvaI CYCGRG 1 cut(s) 246
BaeGI GKGCMC 1 cut(s) 38
BalI TGGCCA 1 cut(s) 287
BanI GGYRCC 1 cut(s) 366
BanII GRGCYC 1 cut(s) 258
BbrPI CACGTG 1 cut(s) 646
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 2 cut(s) 38, 258
BbvI GCAGC 1 cut(s) 294
BccI CCATC 2 cut(s) 323, 415
BceAI ACGGC 5 cut(s) 208, 226, 308, 524, 543
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 2 cut(s) 14, 578
BfaI CTAG 1 cut(s) 714
BfmI CTRYAG 1 cut(s) 725
BisI GCNGC 9 cut(s) 150, 153, 308, 345, 486, 489, 494, 531, 597
BlsI GCNGC 9 cut(s) 151, 154, 309, 346, 487, 490, 495, 532, 598
BmcAI AGTACT 1 cut(s) 712
Bme1390I CCNGG 1 cut(s) 84
BmeT110I CYCGRG 1 cut(s) 246
BmgBI CACGTC 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 80, 228
BmiI GGNNCC 1 cut(s) 368
BmrFI CCNGG 1 cut(s) 84
BmrI ACTGGG 2 cut(s) 87, 235
BmsI GCATC 2 cut(s) 106, 684
BmuI ACTGGG 2 cut(s) 87, 235
BoxI GACNNNNGTC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 52
BplI GAGNNNNNCTC 2 cut(s) 240, 272
BpuMI CCSGG 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 646
BsaBI GATNNNNATC 1 cut(s) 425
BsaJI CCNNGG 3 cut(s) 247, 540, 664
BsaWI WCCGGW 1 cut(s) 273
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 273
Bse118I RCCGGY 2 cut(s) 329, 347
Bse1I ACTGG 3 cut(s) 82, 117, 230
Bse8I GATNNNNATC 1 cut(s) 425
BseDI CCNNGG 3 cut(s) 247, 540, 664
BseGI GGATG 4 cut(s) 63, 121, 161, 354
BseJI GATNNNNATC 1 cut(s) 425
BseLI CCNNNNNNNGG 2 cut(s) 78, 273
BseMII CTCAG 2 cut(s) 523, 668
BseNI ACTGG 3 cut(s) 82, 117, 230
BseRI GAGGAG 2 cut(s) 596, 629
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 1 cut(s) 294
Bsh1236I CGCG 6 cut(s) 362, 364, 431, 458, 493, 498
Bsh1285I CGRYCG 2 cut(s) 278, 282
BshFI GGCC 5 cut(s) 82, 230, 287, 333, 539
BshNI GGYRCC 1 cut(s) 366
BsiEI CGRYCG 2 cut(s) 278, 282
BsiHKAI GWGCWC 2 cut(s) 38, 258
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 6 cut(s) 83, 231, 274, 330, 348, 443
BslFI GGGAC 1 cut(s) 654
BslI CCNNNNNNNGG 2 cut(s) 78, 273
BsmAI GTCTC 2 cut(s) 14, 578
BsmBI CGTCTC 1 cut(s) 578
BsmFI GGGAC 1 cut(s) 654
BsnI GGCC 5 cut(s) 82, 230, 287, 333, 539
BsoBI CYCGRG 1 cut(s) 246
Bsp1286I GDGCHC 2 cut(s) 38, 258
Bsp143I GATC 4 cut(s) 177, 217, 233, 716
BspANI GGCC 5 cut(s) 82, 230, 287, 333, 539
BspCNI CTCAG 2 cut(s) 522, 667
BspFNI CGCG 6 cut(s) 362, 364, 431, 458, 493, 498
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 368
BspPI GGATC 2 cut(s) 241, 711
BspT107I GGYRCC 1 cut(s) 366
BsrBI CCGCTC 1 cut(s) 515
BsrFI RCCGGY 2 cut(s) 329, 347
BsrI ACTGG 3 cut(s) 82, 117, 230
BssAI RCCGGY 2 cut(s) 329, 347
BssECI CCNNGG 3 cut(s) 247, 540, 664
BssMI GATC 4 cut(s) 177, 217, 233, 716
BssT1I CCWWGG 1 cut(s) 664
Bst4CI ACNGT 4 cut(s) 142, 589, 710, 726
BstBAI YACGTR 1 cut(s) 646
BstC8I GCNNGC 2 cut(s) 254, 349
BstDEI CTNAG 3 cut(s) 24, 509, 654
BstDSI CCRYGG 1 cut(s) 540
BstF5I GGATG 4 cut(s) 63, 121, 161, 354
BstFNI CGCG 6 cut(s) 362, 364, 431, 458, 493, 498
BstHHI GCGC 5 cut(s) 364, 433, 460, 493, 498
BstKTI GATC 4 cut(s) 180, 220, 236, 719
BstMAI GTCTC 2 cut(s) 14, 578
BstMBI GATC 4 cut(s) 177, 217, 233, 716
BstMCI CGRYCG 2 cut(s) 278, 282
BstMWI GCNNNNNNNGC 3 cut(s) 88, 344, 536
BstPAI GACNNNNGTC 1 cut(s) 317
BstSCI CCNGG 1 cut(s) 82
BstSFI CTRYAG 1 cut(s) 725
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 6 cut(s) 362, 364, 431, 458, 493, 498
BstV1I GCAGC 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 716
BstYI RGATCY 1 cut(s) 716
BsuRI GGCC 5 cut(s) 82, 230, 287, 333, 539
BtgI CCRYGG 1 cut(s) 540
BtgZI GCGATG 2 cut(s) 412, 707
BtrI CACGTC 1 cut(s) 159
BtsCI GGATG 4 cut(s) 63, 121, 161, 354
Cac8I GCNNGC 2 cut(s) 254, 349
CciI TCATGA 1 cut(s) 258
CfoI GCGC 5 cut(s) 364, 433, 460, 493, 498
Cfr10I RCCGGY 2 cut(s) 329, 347
Cfr13I GGNCC 2 cut(s) 80, 228
Csp6I GTAC 2 cut(s) 649, 711
CviAII CATG 3 cut(s) 259, 412, 704
CviQI GTAC 2 cut(s) 649, 711
DdeI CTNAG 3 cut(s) 24, 509, 654
DpnI GATC 4 cut(s) 179, 219, 235, 718
DpnII GATC 4 cut(s) 177, 217, 233, 716
DraI TTTAAA 1 cut(s) 135
EaeI YGGCCR 3 cut(s) 285, 331, 537
EciI GGCGGA 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 256
Eco72I CACGTG 1 cut(s) 646
Eco88I CYCGRG 1 cut(s) 246
EcoICRI GAGCTC 1 cut(s) 256
EcoT14I CCWWGG 1 cut(s) 664
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 664
Esp3I CGTCTC 1 cut(s) 578
FaeI CATG 3 cut(s) 262, 415, 707
FaiI YATR 6 cut(s) 93, 260, 378, 386, 413, 705
FaqI GGGAC 1 cut(s) 654
FatI CATG 3 cut(s) 258, 411, 703
FauI CCCGC 1 cut(s) 312
FbaI TGATCA 1 cut(s) 177
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 9 cut(s) 150, 153, 308, 345, 486, 489, 494, 531, 597
FokI GGATG 4 cut(s) 50, 128, 148, 341
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 9 cut(s) 150, 153, 308, 345, 486, 489, 494, 531, 597
FspBI CTAG 1 cut(s) 714
GlaI GCGC 5 cut(s) 363, 432, 459, 492, 497
GluI GCNGC 9 cut(s) 150, 153, 308, 345, 486, 489, 494, 531, 597
HaeIII GGCC 5 cut(s) 82, 230, 287, 333, 539
HapII CCGG 6 cut(s) 83, 231, 274, 330, 348, 443
HhaI GCGC 5 cut(s) 364, 433, 460, 493, 498
Hin1II CATG 3 cut(s) 262, 415, 707
Hin6I GCGC 5 cut(s) 362, 431, 458, 491, 496
HinP1I GCGC 5 cut(s) 362, 431, 458, 491, 496
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 3 cut(s) 439, 560, 658
HpaII CCGG 6 cut(s) 83, 231, 274, 330, 348, 443
HphI GGTGA 5 cut(s) 61, 98, 172, 319, 448
Hpy166II GTNNAC 5 cut(s) 36, 409, 454, 559, 649
Hpy188I TCNGA 5 cut(s) 146, 238, 512, 657, 679
Hpy188III TCNNGA 2 cut(s) 221, 259
Hpy8I GTNNAC 5 cut(s) 36, 409, 454, 559, 649
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 3 cut(s) 385, 541, 631
HpyCH4III ACNGT 4 cut(s) 142, 589, 710, 726
HpyCH4IV ACGT 3 cut(s) 38, 158, 645
HpyCH4V TGCA 3 cut(s) 4, 36, 119
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 344, 536
HpyF3I CTNAG 3 cut(s) 24, 509, 654
HpySE526I ACGT 3 cut(s) 38, 158, 645
Hsp92II CATG 3 cut(s) 262, 415, 707
HspAI GCGC 5 cut(s) 362, 431, 458, 491, 496
KroI GCCGGC 1 cut(s) 347
KroNI GCCGGC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 4 cut(s) 177, 217, 233, 716
LmnI GCTCC 1 cut(s) 276
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 2 cut(s) 106, 684
MaeI CTAG 1 cut(s) 714
MaeII ACGT 3 cut(s) 38, 158, 645
MaeIII GTNAC 1 cut(s) 325
MalI GATC 4 cut(s) 179, 219, 235, 718
MbiI CCGCTC 1 cut(s) 515
MboI GATC 4 cut(s) 177, 217, 233, 716
MboII GAAGA 2 cut(s) 52, 193
MflI RGATCY 1 cut(s) 716
MhlI GDGCHC 2 cut(s) 38, 258
MlsI TGGCCA 1 cut(s) 287
MluCI AATT 1 cut(s) 197
MluNI TGGCCA 1 cut(s) 287
MlyI GAGTC 1 cut(s) 554
MmeI TCCRAC 3 cut(s) 21, 231, 657
Mox20I TGGCCA 1 cut(s) 287
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 347
MscI TGGCCA 1 cut(s) 287
MseI TTAA 3 cut(s) 134, 465, 570
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 6 cut(s) 83, 231, 274, 330, 348, 443
MspR9I CCNGG 1 cut(s) 84
MteI GCGCNGCGC 1 cut(s) 494
MvnI CGCG 6 cut(s) 362, 364, 431, 458, 493, 498
MwoI GCNNNNNNNGC 3 cut(s) 88, 344, 536
NaeI GCCGGC 1 cut(s) 349
NciI CCSGG 1 cut(s) 84
NdeII GATC 4 cut(s) 177, 217, 233, 716
NgoMIV GCCGGC 1 cut(s) 347
NlaIII CATG 3 cut(s) 262, 415, 707
NlaIV GGNNCC 1 cut(s) 368
NmeAIII GCCGAG 1 cut(s) 542
NmuCI GTSAC 1 cut(s) 325
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 258
PdiI GCCGGC 1 cut(s) 349
PfeI GAWTC 2 cut(s) 439, 658
PkrI GCNGC 9 cut(s) 151, 154, 309, 346, 487, 490, 495, 532, 598
PleI GAGTC 1 cut(s) 554
PmaCI CACGTG 1 cut(s) 646
PmlI CACGTG 1 cut(s) 646
PpsI GAGTC 1 cut(s) 554
Ppu21I YACGTR 1 cut(s) 646
PshAI GACNNNNGTC 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 258
PspCI CACGTG 1 cut(s) 646
PspN4I GGNNCC 1 cut(s) 368
PspPI GGNCC 2 cut(s) 80, 228
PsuI RGATCY 1 cut(s) 716
RsaI GTAC 2 cut(s) 650, 712
RsaNI GTAC 2 cut(s) 649, 711
RseI CAYNNNNRTG 1 cut(s) 375
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 3 cut(s) 134, 465, 570
SatI GCNGC 9 cut(s) 150, 153, 308, 345, 486, 489, 494, 531, 597
Sau3AI GATC 4 cut(s) 177, 217, 233, 716
Sau96I GGNCC 2 cut(s) 80, 228
ScaI AGTACT 1 cut(s) 712
SchI GAGTC 1 cut(s) 554
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 2 cut(s) 38, 258
SetI ASST 9 cut(s) 41, 60, 161, 258, 266, 273, 477, 648, 684
SfaNI GCATC 2 cut(s) 106, 684
SfcI CTRYAG 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 375
Sse9I AATT 1 cut(s) 197
SspMI CTAG 1 cut(s) 714
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 1 cut(s) 664
TaaI ACNGT 4 cut(s) 142, 589, 710, 726
TaiI ACGT 3 cut(s) 41, 161, 648
TaqI TCGA 1 cut(s) 216
TaqII GACCGA 1 cut(s) 268
TasI AATT 1 cut(s) 197
TatI WGTACW 1 cut(s) 710
TauI GCSGC 8 cut(s) 152, 155, 347, 488, 491, 496, 533, 599
TfiI GAWTC 2 cut(s) 439, 658
Tru1I TTAA 3 cut(s) 134, 465, 570
Tru9I TTAA 3 cut(s) 134, 465, 570
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 1 cut(s) 307
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 3 cut(s) 52, 283, 343
TspGWI ACGGA 1 cut(s) 157
VneI GTGCAC 1 cut(s) 34
XapI RAATTY 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 408
XspI CTAG 1 cut(s) 714
ZrmI AGTACT 1 cut(s) 712
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.