MD01G1141800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007099
Physical Location & Seq
Reverse (-)
25134509 .. 25138170
3662 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1141800.v1.1.491

Sequence Viewer

Length: 921 bp
ATGCCCGGCGGAACCACCATCCACCTGACAGCCCTCGACGGCATCGTCAACGTCAACTCCCTCTTCACCTTAGCCGTCTTCATCGGCCTCACCTGGAACCCTTCCGACCCGGAAAACACCCTCATCGAGGCCGACGACCCCATCGCCTGCCGCCCTGGTCCCTCCGTCGCCGAGGATCTCGTCTCCTTCCACGTCTACTCCTTCAGCTCCTTCCTCTTCTCCAGCCTCGTCGCCCTCGGCCTCAAGCAGGCCATCCGGATCACCCGCAGCCCGTCCTACCACCGGCCCGCCGAGATTCTCGTCCGGGTCAACAGCACCCTCCTCCGGATCGGGATGCTCGTCTCGGGTGTCGGTTCGGCGGCCGGTTGCGTGTTCCTGATGCTGGCTCTCATCAACGTGGTGCAGATCAAGCTCGGGACTTTGGCCTGCGGCAGCTTCAACACTCTCGCCGCCGTCGTCCCGCTGGTCATCTTTGTCTCCGTCGCGCTGCTCATCTATGTTTGCACCGTTTTTGCTGCACTTGGTGCAAATGCGAAAATAGACATGGAAGGAGGTGCGATATTGAGGAAGTTCTGGTGCTTGGTGGTGGCTTTGTTTGTAATTTCAGGCAAGAATTGGGGATTAATTGAAGGGGTTGATGGGGCTGCCGGCGGTGACTGTGGGGAGATGGACCCGGACGTTCTGGTGTACAAGCTAGCATCGTGTGCGGCGTCGTCACAGGACAAGAACGTGGCGGTTCCTAACAAGTGCTGTGCTGTGGTGAGCAAGGTACGCACAAACTGCCTTTGCTCCATCTTACTTTCTAAAGAAGCCAAGAGCCTTGGAATTAGGCCTGATGTTGCAGCCACTATTCCCAAACGTTGCAATGTTGCCAGACGTACTAGGGGCTACAAGTGCGGAGCTTTTATAGTGCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

32.21

Weight (kDa)

8.68

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 229 - 294 6.4e-08 Probable lipid transfer
Tryp_alpha_amyl PF00234 231 - 299 1.5e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AccI GTMKAC 1 cut(s) 195
AccII CGCG 1 cut(s) 485
AccIII TCCGGA 2 cut(s) 255, 324
AclI AACGTT 1 cut(s) 859
AclWI GGATC 3 cut(s) 183, 266, 335
AcoI YGGCCR 1 cut(s) 360
AcuI CTGAAG 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 710
AdeI CACNNNGTG 1 cut(s) 524
AfaI GTAC 3 cut(s) 689, 771, 880
AfiI CCNNNNNNNGG 5 cut(s) 127, 247, 282, 324, 382
AgsI TTSAA 2 cut(s) 439, 629
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 2 cut(s) 92, 154
AluBI AGCT 6 cut(s) 207, 412, 435, 694, 902, 917
AluI AGCT 6 cut(s) 207, 412, 435, 694, 902, 917
Alw26I GTCTC 3 cut(s) 187, 346, 481
AlwI GGATC 3 cut(s) 183, 266, 335
Ama87I CYCGRG 2 cut(s) 343, 413
Aor13HI TCCGGA 2 cut(s) 255, 324
AoxI GGCC 8 cut(s) 85, 129, 238, 249, 284, 360, 423, 830
ApeKI GCWGC 6 cut(s) 267, 432, 487, 515, 644, 842
AseI ATTAAT 1 cut(s) 623
AspLEI GCGC 1 cut(s) 487
AspS9I GGNCC 3 cut(s) 158, 285, 670
AsuC2I CCSGG 4 cut(s) 6, 110, 305, 674
AsuHPI GGTGA 5 cut(s) 58, 82, 253, 665, 772
AsuNHI GCTAGC 1 cut(s) 694
AvaI CYCGRG 2 cut(s) 343, 413
AvaII GGWCC 2 cut(s) 158, 670
BbsI GAAGAC 1 cut(s) 70
BbvI GCAGC 6 cut(s) 279, 444, 474, 502, 631, 854
BccI CCATC 6 cut(s) 26, 149, 260, 632, 661, 800
BceAI ACGGC 3 cut(s) 55, 59, 437
BciT130I CCWGG 2 cut(s) 94, 156
BcnI CCSGG 4 cut(s) 6, 110, 305, 674
BcoDI GTCTC 3 cut(s) 187, 346, 481
BfaI CTAG 2 cut(s) 695, 882
Bme1390I CCNGG 6 cut(s) 6, 94, 110, 156, 305, 674
Bme18I GGWCC 2 cut(s) 158, 670
BmeT110I CYCGRG 2 cut(s) 343, 413
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 3 cut(s) 158, 285, 670
BmiI GGNNCC 5 cut(s) 13, 98, 160, 672, 738
BmrFI CCNGG 6 cut(s) 6, 94, 110, 156, 305, 674
BmsI GCATC 4 cut(s) 51, 324, 369, 707
BmtI GCTAGC 1 cut(s) 698
BpiI GAAGAC 1 cut(s) 70
BpmI CTGGAG 1 cut(s) 205
Bpu10I CCTNAGC 1 cut(s) 70
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 4 cut(s) 6, 110, 305, 674
BsaHI GRCGYC 1 cut(s) 710
BsaJI CCNNGG 4 cut(s) 154, 171, 235, 820
BsaWI WCCGGW 2 cut(s) 255, 324
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 182, 212
Bsc4I CCNNNNNNNGG 5 cut(s) 127, 247, 282, 324, 382
Bse118I RCCGGY 3 cut(s) 282, 362, 647
Bse3DI GCAATG 1 cut(s) 871
BseAI TCCGGA 2 cut(s) 255, 324
BseBI CCWGG 2 cut(s) 94, 156
BseDI CCNNGG 4 cut(s) 154, 171, 235, 820
BseGI GGATG 3 cut(s) 18, 252, 339
BseLI CCNNNNNNNGG 5 cut(s) 127, 247, 282, 324, 382
BseMI GCAATG 1 cut(s) 871
BseRI GAGGAG 1 cut(s) 311
BseX3I CGGCCG 1 cut(s) 360
BseXI GCAGC 6 cut(s) 279, 444, 474, 502, 631, 854
BsgI GTGCAG 2 cut(s) 422, 501
Bsh1236I CGCG 1 cut(s) 485
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 8 cut(s) 87, 131, 240, 251, 286, 362, 425, 832
BsiEI CGRYCG 1 cut(s) 363
BsiHKCI CYCGRG 2 cut(s) 343, 413
BsiSI CCGG 9 cut(s) 6, 110, 256, 283, 304, 325, 363, 648, 674
BslFI GGGAC 3 cut(s) 144, 430, 443
BslI CCNNNNNNNGG 5 cut(s) 127, 247, 282, 324, 382
BsmAI GTCTC 3 cut(s) 187, 346, 481
BsmBI CGTCTC 2 cut(s) 187, 346
BsmFI GGGAC 3 cut(s) 144, 430, 443
BsnI GGCC 8 cut(s) 87, 131, 240, 251, 286, 362, 425, 832
BsoBI CYCGRG 2 cut(s) 343, 413
Bsp13I TCCGGA 2 cut(s) 255, 324
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 4 cut(s) 175, 258, 327, 405
BspANI GGCC 8 cut(s) 87, 131, 240, 251, 286, 362, 425, 832
BspEI TCCGGA 2 cut(s) 255, 324
BspFNI CGCG 1 cut(s) 485
BspLI GGNNCC 5 cut(s) 13, 98, 160, 672, 738
BspOI GCTAGC 1 cut(s) 698
BspPI GGATC 3 cut(s) 183, 266, 335
BsrDI GCAATG 1 cut(s) 871
BsrFI RCCGGY 3 cut(s) 282, 362, 647
BsrGI TGTACA 1 cut(s) 687
BssAI RCCGGY 3 cut(s) 282, 362, 647
BssECI CCNNGG 4 cut(s) 154, 171, 235, 820
BssMI GATC 4 cut(s) 175, 258, 327, 405
BssNI GRCGYC 1 cut(s) 710
BssT1I CCWWGG 1 cut(s) 820
Bst2UI CCWGG 2 cut(s) 94, 156
Bst4CI ACNGT 2 cut(s) 508, 659
Bst6I CTCTTC 2 cut(s) 68, 221
BstACI GRCGYC 1 cut(s) 710
BstAPI GCANNNNNTGC 3 cut(s) 524, 704, 780
BstAUI TGTACA 1 cut(s) 687
BstC8I GCNNGC 8 cut(s) 148, 249, 288, 384, 427, 649, 696, 915
BstDEI CTNAG 1 cut(s) 70
BstENI CCTNNNNNAGG 2 cut(s) 125, 245
BstF5I GGATG 3 cut(s) 18, 252, 339
BstFNI CGCG 1 cut(s) 485
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 4 cut(s) 178, 261, 330, 408
BstMAI GTCTC 3 cut(s) 187, 346, 481
BstMBI GATC 4 cut(s) 175, 258, 327, 405
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 6 cut(s) 409, 524, 704, 771, 780, 894
BstNI CCWGG 2 cut(s) 94, 156
BstSCI CCNGG 6 cut(s) 4, 92, 108, 154, 303, 672
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 6 cut(s) 279, 444, 474, 502, 631, 854
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 360
BsuRI GGCC 8 cut(s) 87, 131, 240, 251, 286, 362, 425, 832
BtgZI GCGATG 1 cut(s) 127
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 3 cut(s) 18, 252, 339
Cac8I GCNNGC 8 cut(s) 148, 249, 288, 384, 427, 649, 696, 915
CfoI GCGC 1 cut(s) 487
Cfr10I RCCGGY 3 cut(s) 282, 362, 647
Cfr13I GGNCC 3 cut(s) 158, 285, 670
CseI GACGC 1 cut(s) 699
Csp6I GTAC 3 cut(s) 688, 770, 879
CviAII CATG 1 cut(s) 544
CviQI GTAC 3 cut(s) 688, 770, 879
DdeI CTNAG 1 cut(s) 70
DpnI GATC 4 cut(s) 177, 260, 329, 407
DpnII GATC 4 cut(s) 175, 258, 327, 405
DraIII CACNNNGTG 1 cut(s) 524
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
EaeI YGGCCR 1 cut(s) 360
EagI CGGCCG 1 cut(s) 360
Eam1104I CTCTTC 2 cut(s) 68, 221
EarI CTCTTC 2 cut(s) 68, 221
EciI GGCGGA 1 cut(s) 24
EclXI CGGCCG 1 cut(s) 360
Eco130I CCWWGG 1 cut(s) 820
Eco147I AGGCCT 1 cut(s) 832
Eco47I GGWCC 2 cut(s) 158, 670
Eco52I CGGCCG 1 cut(s) 360
Eco57I CTGAAG 1 cut(s) 187
Eco88I CYCGRG 2 cut(s) 343, 413
EcoNI CCTNNNNNAGG 2 cut(s) 125, 245
EcoRII CCWGG 2 cut(s) 92, 154
EcoT14I CCWWGG 1 cut(s) 820
ErhI CCWWGG 1 cut(s) 820
Esp3I CGTCTC 2 cut(s) 187, 346
FaeI CATG 1 cut(s) 547
FaiI YATR 3 cut(s) 498, 545, 908
FaqI GGGAC 3 cut(s) 144, 430, 443
FatI CATG 1 cut(s) 543
FauI CCCGC 3 cut(s) 272, 295, 468
FblI GTMKAC 1 cut(s) 195
FokI GGATG 3 cut(s) 5, 239, 346
FspBI CTAG 2 cut(s) 695, 882
GlaI GCGC 1 cut(s) 486
GsuI CTGGAG 1 cut(s) 205
HaeIII GGCC 8 cut(s) 87, 131, 240, 251, 286, 362, 425, 832
HapII CCGG 9 cut(s) 6, 110, 256, 283, 304, 325, 363, 648, 674
HgaI GACGC 1 cut(s) 699
HhaI GCGC 1 cut(s) 487
Hin1I GRCGYC 1 cut(s) 710
Hin1II CATG 1 cut(s) 547
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HincII GTYRAC 3 cut(s) 49, 55, 310
HindII GTYRAC 3 cut(s) 49, 55, 310
HinfI GANTC 1 cut(s) 295
HpaII CCGG 9 cut(s) 6, 110, 256, 283, 304, 325, 363, 648, 674
HphI GGTGA 5 cut(s) 58, 82, 253, 665, 772
Hpy166II GTNNAC 5 cut(s) 49, 55, 196, 310, 688
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 5 cut(s) 256, 325, 331, 376, 415
Hpy8I GTNNAC 5 cut(s) 49, 55, 196, 310, 688
Hpy99I CGWCG 7 cut(s) 41, 137, 170, 233, 458, 485, 715
HpyAV CCTTC 6 cut(s) 111, 196, 211, 220, 542, 623
HpyCH4III ACNGT 2 cut(s) 508, 659
HpyCH4IV ACGT 7 cut(s) 51, 192, 396, 678, 729, 859, 877
HpyCH4V TGCA 6 cut(s) 403, 504, 518, 527, 842, 864
HpyF10VI GCNNNNNNNGC 6 cut(s) 409, 524, 704, 771, 780, 894
HpyF3I CTNAG 1 cut(s) 70
HpySE526I ACGT 7 cut(s) 51, 192, 396, 678, 729, 859, 877
Hsp92I GRCGYC 1 cut(s) 710
Hsp92II CATG 1 cut(s) 547
HspAI GCGC 1 cut(s) 485
Kpn2I TCCGGA 2 cut(s) 255, 324
KroI GCCGGC 1 cut(s) 647
KroNI GCCGGC 1 cut(s) 649
Kzo9I GATC 4 cut(s) 175, 258, 327, 405
LmnI GCTCC 3 cut(s) 212, 794, 899
Lsp1109I GCAGC 6 cut(s) 279, 444, 474, 502, 631, 854
LweI GCATC 4 cut(s) 51, 324, 369, 707
MaeI CTAG 2 cut(s) 695, 882
MaeII ACGT 7 cut(s) 51, 192, 396, 678, 729, 859, 877
MaeIII GTNAC 2 cut(s) 653, 714
MalI GATC 4 cut(s) 177, 260, 329, 407
MboI GATC 4 cut(s) 175, 258, 327, 405
MboII GAAGA 3 cut(s) 55, 70, 208
MflI RGATCY 1 cut(s) 175
MluCI AATT 4 cut(s) 600, 613, 624, 825
MmeI TCCRAC 1 cut(s) 129
MroI TCCGGA 2 cut(s) 255, 324
MroNI GCCGGC 1 cut(s) 647
MseI TTAA 1 cut(s) 623
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 463
MspI CCGG 9 cut(s) 6, 110, 256, 283, 304, 325, 363, 648, 674
MspR9I CCNGG 6 cut(s) 6, 94, 110, 156, 305, 674
MvaI CCWGG 2 cut(s) 94, 156
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 6 cut(s) 409, 524, 704, 771, 780, 894
NaeI GCCGGC 1 cut(s) 649
NciI CCSGG 4 cut(s) 6, 110, 305, 674
NdeII GATC 4 cut(s) 175, 258, 327, 405
NgoMIV GCCGGC 1 cut(s) 647
NheI GCTAGC 1 cut(s) 694
NlaIII CATG 1 cut(s) 547
NlaIV GGNNCC 5 cut(s) 13, 98, 160, 672, 738
NmeAIII GCCGAG 3 cut(s) 196, 216, 316
NmuCI GTSAC 2 cut(s) 653, 714
PceI AGGCCT 1 cut(s) 832
PcsI WCGNNNNNNNCGW 5 cut(s) 42, 132, 336, 453, 707
PdiI GCCGGC 1 cut(s) 649
PfeI GAWTC 1 cut(s) 295
PshBI ATTAAT 1 cut(s) 623
Psp1406I AACGTT 1 cut(s) 859
Psp6I CCWGG 2 cut(s) 92, 154
PspGI CCWGG 2 cut(s) 92, 154
PspN4I GGNNCC 5 cut(s) 13, 98, 160, 672, 738
PspPI GGNCC 3 cut(s) 158, 285, 670
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 3 cut(s) 689, 771, 880
RsaNI GTAC 3 cut(s) 688, 770, 879
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 1 cut(s) 623
Sau3AI GATC 4 cut(s) 175, 258, 327, 405
Sau96I GGNCC 3 cut(s) 158, 285, 670
ScrFI CCNGG 6 cut(s) 6, 94, 110, 156, 305, 674
SfaNI GCATC 4 cut(s) 51, 324, 369, 707
SinI GGWCC 2 cut(s) 158, 670
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 4 cut(s) 600, 613, 624, 825
SseBI AGGCCT 1 cut(s) 832
SspMI CTAG 2 cut(s) 695, 882
StuI AGGCCT 1 cut(s) 832
StyD4I CCNGG 6 cut(s) 4, 92, 108, 154, 303, 672
StyI CCWWGG 1 cut(s) 820
TaaI ACNGT 2 cut(s) 508, 659
TaiI ACGT 7 cut(s) 54, 195, 399, 681, 732, 862, 880
TaqI TCGA 2 cut(s) 36, 126
TasI AATT 4 cut(s) 600, 613, 624, 825
TatI WGTACW 1 cut(s) 687
TauI GCSGC 5 cut(s) 153, 362, 432, 452, 710
TfiI GAWTC 1 cut(s) 295
Tru1I TTAA 1 cut(s) 623
Tru9I TTAA 1 cut(s) 623
TseFI GTSAC 2 cut(s) 653, 714
TseI GCWGC 6 cut(s) 267, 432, 487, 515, 644, 842
Tsp45I GTSAC 2 cut(s) 653, 714
TspDTI ATGAA 1 cut(s) 70
TspGWI ACGGA 2 cut(s) 154, 469
VpaK11BI GGWCC 2 cut(s) 158, 670
VspI ATTAAT 1 cut(s) 623
XagI CCTNNNNNAGG 2 cut(s) 125, 245
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 2 cut(s) 695, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.