Rorug01G0345300

Protease inhibitor seed storage lipid transfer family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007099
Physical Location & Seq
Forward (+)
46288408 .. 46291730
3323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0345300.1

Sequence Viewer

Length: 735 bp
ATGGCGAGGACGGCTCTCCTTCACTTGCTCCGATCACACTCTAAAAACCTCGCTACATCAAATTTCCTTCCAGGTTATCAACCTTGTAAGTCTGGAGCATGGGCACGTGGTCGTGGCCTGAACAACAAACCTAGTTGCAACTCTGGTGTTCTGATCAATGCTTTCCAGAAGAGATGGGTGTCTCAAGCTGCAACAAGAGAAGATGATGGCAAAATCAGCATTGGACCACGTAGAGGTGTTGAAACTGGGAAAGATGAAAAAGATTCTGGGATTGTATACTATGGTCCGATCTCATCTACTATCAAGAAAGTGAAACTTCTCTCGCTTTCAACCTGCTGCCTTTCTGTATCTTTAGGCCCTGTGATAACCTTTATGACATCACCTGATATGAATGTTATCTTAAAGGGTGCGGTGGCATCTTCAGTGATATTCTTAAGTGCTTCTACCACAGCTGCCCTTCACTGGTTTGTGACCCCATACATTCACAAGCTCAAGTGGAAGCCTGGTTCAGACAGTTTTGAGGTCGAAATGTTGTCATGGCTAGCAACTTACATACCAAGGACAATTAAGTTCGCTGATATCCGGCCGCCAGAAACCCAAAGGCCCTTTGTGACTTTCAAGGCCAATGGACAGTTTTACTTCGTTGATGCTGATCATTGTCAGAATAAGGCTTTGTTAGCAAAGCTGACCCCTCAGAAAGCACCTACTCGTGAGTCTGCTTTTAAGAACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

244

Amino Acids

26.91

Weight (kDa)

9.83

Isoelectric Point (pI)

28.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM70 PF06979 91 - 228 5.1e-11 TMEM70/TMEM186/TMEM223 protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 341
AccI GTMKAC 1 cut(s) 276
AciI CCGC 2 cut(s) 410, 587
AcoI YGGCCR 1 cut(s) 584
AcsI RAATTY 1 cut(s) 61
AcuI CTGAAG 1 cut(s) 405
AcvI CACGTG 1 cut(s) 107
AfiI CCNNNNNNNGG 2 cut(s) 233, 462
AflII CTTAAG 1 cut(s) 433
AgsI TTSAA 3 cut(s) 242, 330, 619
AjnI CCWGG 2 cut(s) 70, 502
AloI GAACNNNNNNTCC 2 cut(s) 490, 522
AluBI AGCT 4 cut(s) 188, 452, 490, 685
AluI AGCT 4 cut(s) 188, 452, 490, 685
Alw26I GTCTC 1 cut(s) 186
AoxI GGCC 5 cut(s) 115, 355, 584, 602, 621
ApeKI GCWGC 3 cut(s) 188, 336, 452
ApoI RAATTY 1 cut(s) 61
AspS9I GGNCC 4 cut(s) 224, 284, 356, 603
AsuHPI GGTGA 1 cut(s) 372
AsuNHI GCTAGC 1 cut(s) 541
AvaII GGWCC 2 cut(s) 224, 284
BaeGI GKGCMC 1 cut(s) 106
BauI CACGAG 1 cut(s) 708
BbrPI CACGTG 1 cut(s) 107
BbvI GCAGC 3 cut(s) 175, 323, 439
BccI CCATC 2 cut(s) 168, 200
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 2 cut(s) 72, 504
BclI TGATCA 2 cut(s) 153, 652
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 2 cut(s) 132, 542
BfrI CTTAAG 1 cut(s) 433
BfuAI ACCTGC 1 cut(s) 341
BisI GCNGC 4 cut(s) 189, 337, 453, 587
BlsI GCNGC 4 cut(s) 190, 338, 454, 588
Bme1390I CCNGG 2 cut(s) 72, 504
Bme18I GGWCC 2 cut(s) 224, 284
BmgT120I GGNCC 4 cut(s) 224, 284, 356, 603
BmrFI CCNGG 2 cut(s) 72, 504
BmrI ACTGGG 1 cut(s) 255
BmsI GCATC 2 cut(s) 425, 637
BmtI GCTAGC 1 cut(s) 545
BmuI ACTGGG 1 cut(s) 255
BplI GAGNNNNNCTC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 114
BpuEI CTTGAG 2 cut(s) 168, 476
BsaAI YACGTR 2 cut(s) 107, 230
BsaBI GATNNNNATC 1 cut(s) 651
BsaJI CCNNGG 1 cut(s) 557
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 462
Bse1I ACTGG 2 cut(s) 250, 467
Bse8I GATNNNNATC 1 cut(s) 651
BseBI CCWGG 2 cut(s) 72, 504
BseDI CCNNGG 1 cut(s) 557
BseJI GATNNNNATC 1 cut(s) 651
BseLI CCNNNNNNNGG 2 cut(s) 233, 462
BseMII CTCAG 1 cut(s) 707
BseNI ACTGG 2 cut(s) 250, 467
BseSI GKGCMC 1 cut(s) 106
BseX3I CGGCCG 1 cut(s) 584
BseXI GCAGC 3 cut(s) 175, 323, 439
Bsh1285I CGRYCG 1 cut(s) 587
BshFI GGCC 5 cut(s) 117, 357, 586, 604, 623
BsiEI CGRYCG 1 cut(s) 587
BsiSI CCGG 1 cut(s) 583
BslI CCNNNNNNNGG 2 cut(s) 233, 462
BsmAI GTCTC 1 cut(s) 186
BsnI GGCC 5 cut(s) 117, 357, 586, 604, 623
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 4 cut(s) 32, 153, 288, 652
BspACI CCGC 2 cut(s) 410, 587
BspANI GGCC 5 cut(s) 117, 357, 586, 604, 623
BspCNI CTCAG 1 cut(s) 706
BspMI ACCTGC 1 cut(s) 341
BspOI GCTAGC 1 cut(s) 545
BspTI CTTAAG 1 cut(s) 433
BsrI ACTGG 2 cut(s) 250, 467
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 4 cut(s) 32, 153, 288, 652
BssNAI GTATAC 1 cut(s) 277
BssSI CACGAG 1 cut(s) 708
BssT1I CCWWGG 1 cut(s) 557
Bst1107I GTATAC 1 cut(s) 277
Bst2BI CACGAG 1 cut(s) 708
Bst2UI CCWGG 2 cut(s) 72, 504
Bst4CI ACNGT 2 cut(s) 515, 633
Bst6I CTCTTC 1 cut(s) 164
BstAFI CTTAAG 1 cut(s) 433
BstBAI YACGTR 2 cut(s) 107, 230
BstC8I GCNNGC 1 cut(s) 543
BstDEI CTNAG 1 cut(s) 693
BstKTI GATC 4 cut(s) 35, 156, 291, 655
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 4 cut(s) 32, 153, 288, 652
BstMCI CGRYCG 1 cut(s) 587
BstMWI GCNNNNNNNGC 3 cut(s) 11, 216, 677
BstNI CCWGG 2 cut(s) 72, 504
BstSCI CCNGG 2 cut(s) 70, 502
BstSLI GKGCMC 1 cut(s) 106
BstV1I GCAGC 3 cut(s) 175, 323, 439
BstZ17I GTATAC 1 cut(s) 277
BstZI CGGCCG 1 cut(s) 584
BsuRI GGCC 5 cut(s) 117, 357, 586, 604, 623
BtsIMutI CAGTG 2 cut(s) 429, 460
BveI ACCTGC 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 543
Cfr13I GGNCC 4 cut(s) 224, 284, 356, 603
CviAII CATG 2 cut(s) 99, 537
DdeI CTNAG 1 cut(s) 693
DpnI GATC 4 cut(s) 34, 155, 290, 654
DpnII GATC 4 cut(s) 32, 153, 288, 652
EaeI YGGCCR 1 cut(s) 584
EagI CGGCCG 1 cut(s) 584
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EclXI CGGCCG 1 cut(s) 584
Eco130I CCWWGG 1 cut(s) 557
Eco32I GATATC 1 cut(s) 580
Eco47I GGWCC 2 cut(s) 224, 284
Eco52I CGGCCG 1 cut(s) 584
Eco57I CTGAAG 1 cut(s) 405
Eco72I CACGTG 1 cut(s) 107
EcoO109I RGGNCCY 2 cut(s) 356, 603
EcoRII CCWGG 2 cut(s) 70, 502
EcoRV GATATC 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 557
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 2 cut(s) 102, 540
FaiI YATR 8 cut(s) 100, 277, 282, 374, 389, 478, 538, 554
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FatI CATG 2 cut(s) 98, 536
FbaI TGATCA 2 cut(s) 153, 652
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 4 cut(s) 189, 337, 453, 587
Fsp4HI GCNGC 4 cut(s) 189, 337, 453, 587
FspBI CTAG 2 cut(s) 132, 542
GluI GCNGC 4 cut(s) 189, 337, 453, 587
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 5 cut(s) 117, 357, 586, 604, 623
HapII CCGG 1 cut(s) 583
Hin1II CATG 2 cut(s) 102, 540
HinfI GANTC 2 cut(s) 263, 713
HpaII CCGG 1 cut(s) 583
HphI GGTGA 1 cut(s) 372
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 6 cut(s) 32, 153, 288, 511, 663, 696
Hpy188III TCNNGA 4 cut(s) 93, 166, 304, 710
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 3 cut(s) 29, 77, 467
HpyCH4III ACNGT 2 cut(s) 515, 633
HpyCH4IV ACGT 2 cut(s) 106, 229
HpyCH4V TGCA 2 cut(s) 138, 191
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 216, 677
HpyF3I CTNAG 1 cut(s) 693
HpySE526I ACGT 2 cut(s) 106, 229
Hsp92II CATG 2 cut(s) 102, 540
Ksp22I TGATCA 2 cut(s) 153, 652
Kzo9I GATC 4 cut(s) 32, 153, 288, 652
LmnI GCTCC 2 cut(s) 33, 95
Lsp1109I GCAGC 3 cut(s) 175, 323, 439
LweI GCATC 2 cut(s) 425, 637
MaeI CTAG 2 cut(s) 132, 542
MaeII ACGT 2 cut(s) 106, 229
MaeIII GTNAC 2 cut(s) 469, 610
MalI GATC 4 cut(s) 34, 155, 290, 654
MboI GATC 4 cut(s) 32, 153, 288, 652
MboII GAAGA 3 cut(s) 181, 212, 411
MhlI GDGCHC 1 cut(s) 106
MluCI AATT 2 cut(s) 61, 564
MlyI GAGTC 1 cut(s) 722
MnlI CCTC 4 cut(s) 59, 227, 514, 702
MseI TTAA 4 cut(s) 401, 434, 567, 723
MspA1I CMGCKG 1 cut(s) 452
MspCI CTTAAG 1 cut(s) 433
MspI CCGG 1 cut(s) 583
MspR9I CCNGG 2 cut(s) 72, 504
MvaI CCWGG 2 cut(s) 72, 504
MwoI GCNNNNNNNGC 3 cut(s) 11, 216, 677
NdeII GATC 4 cut(s) 32, 153, 288, 652
NheI GCTAGC 1 cut(s) 541
NlaIII CATG 2 cut(s) 102, 540
NmuCI GTSAC 2 cut(s) 469, 610
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 4 cut(s) 190, 338, 454, 588
PleI GAGTC 1 cut(s) 721
PmaCI CACGTG 1 cut(s) 107
PmlI CACGTG 1 cut(s) 107
PpsI GAGTC 1 cut(s) 721
Ppu21I YACGTR 2 cut(s) 107, 230
Psp6I CCWGG 2 cut(s) 70, 502
PspCI CACGTG 1 cut(s) 107
PspGI CCWGG 2 cut(s) 70, 502
PspPI GGNCC 4 cut(s) 224, 284, 356, 603
PvuII CAGCTG 1 cut(s) 452
SaqAI TTAA 4 cut(s) 401, 434, 567, 723
SatI GCNGC 4 cut(s) 189, 337, 453, 587
Sau3AI GATC 4 cut(s) 32, 153, 288, 652
Sau96I GGNCC 4 cut(s) 224, 284, 356, 603
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 2 cut(s) 72, 504
SduI GDGCHC 1 cut(s) 106
SfaNI GCATC 2 cut(s) 425, 637
SinI GGWCC 2 cut(s) 224, 284
SmlI CTYRAG 3 cut(s) 183, 433, 491
SmoI CTYRAG 3 cut(s) 183, 433, 491
Sse9I AATT 2 cut(s) 61, 564
SsiI CCGC 2 cut(s) 410, 587
SspMI CTAG 2 cut(s) 132, 542
StyD4I CCNGG 2 cut(s) 70, 502
StyI CCWWGG 1 cut(s) 557
TaaI ACNGT 2 cut(s) 515, 633
TaiI ACGT 2 cut(s) 109, 232
TaqI TCGA 1 cut(s) 525
TasI AATT 2 cut(s) 61, 564
TauI GCSGC 1 cut(s) 589
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 4 cut(s) 401, 434, 567, 723
Tru9I TTAA 4 cut(s) 401, 434, 567, 723
TscAI CASTG 2 cut(s) 429, 467
TseFI GTSAC 2 cut(s) 469, 610
TseI GCWGC 3 cut(s) 188, 336, 452
Tsp45I GTSAC 2 cut(s) 469, 610
TspDTI ATGAA 2 cut(s) 270, 404
TspRI CASTG 2 cut(s) 429, 467
Vha464I CTTAAG 1 cut(s) 433
VpaK11BI GGWCC 2 cut(s) 224, 284
XapI RAATTY 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 2 cut(s) 132, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.