pycom01g16210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007099
Physical Location & Seq
Reverse (-)
15858901 .. 15862178
3278 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g16210.1

Sequence Viewer

Length: 894 bp
ATGCCCGGCGGCACCGCCATCCACCTGACGGCCCTCGACGGCATCGTCAACGTCAACTCCCTCTTCACCTTGGCCGTCTTCGTCGGCCTCACCTGGAACCCCTCCGACCCGGAAAACACCCTCATCGAGAACGACGACCCAGCGGCCTGCCGCCCCGGTTCCTCCGTCGCCGAGGATCTCGTCTCCTTCCACGTCTACTCCTTCAGCTCCTTCCTCTTCTCCAGCCTCGTCGCCCTCGGCCTCAAGCAGGCCATCCGGATCACCCGCAGCCCGTCCTACCACCGGCCCGCCGAGATTCTCGTCCGGGTCAACAGCACCCTCCTCCGGATCGGGATGCTCGTCTCGGGTGTCGGTTCGGCGGCCGGTTGCGTGTTCCTGATGCTGGCTCTCGTCAACGTGGTGCAGATCAAGCTCGGGACTTTGGCCTGCGGCAGCTTCAACACTCTCGCCGCCGTCGTCCCGCTCGTCATCCTCGTCCCCGTCGCGCTGCTCATCTATGTTTGCACCAAAATAGACATGGAAGGAGGTGCGATATTGAGGAAGTTCTGGTGCTTGGTGGTGGCTTTGTTTGTAATTTCAGGCAAGAATTGGGGATTAATTGAAGGGGTTGATGGGGCTGCCGGCGGTGAATGTGGGGAGATGGACCCGGACGTTCTGGTGTACAAGCTAGCATCGTGTGCGGCGTCTGCACAGGACAAGAACGTGGCGGTTCCTAACAAGTGCTGTGCTGTGGTTAGCAAGGTACGCGCAAACTGCCTTTGCTCCATCTTACTTTCGAAAGAAGCCAAGAGCCTTGGAATCAGGCCTGATGTTGCAGCCACCATTCCTAAACGTTGCAAAGTTGCCAGACGTACTAGGGGCTACAAGTGCGGAGCTTTTGTCGTGCCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

31.27

Weight (kDa)

8.78

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 220 - 285 4.7e-08 Probable lipid transfer
Tryp_alpha_amyl PF00234 222 - 290 2.6e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 11
AccBSI CCGCTC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 195
AccII CGCG 2 cut(s) 485, 747
AccIII TCCGGA 2 cut(s) 255, 324
AclI AACGTT 1 cut(s) 832
AclWI GGATC 3 cut(s) 183, 266, 335
AcoI YGGCCR 2 cut(s) 72, 360
AcuI CTGAAG 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 683
AfaI GTAC 3 cut(s) 662, 744, 853
AfiI CCNNNNNNNGG 5 cut(s) 28, 247, 282, 324, 382
AgsI TTSAA 2 cut(s) 439, 602
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 5 cut(s) 207, 412, 435, 667, 875
AluI AGCT 5 cut(s) 207, 412, 435, 667, 875
Alw26I GTCTC 2 cut(s) 187, 346
AlwI GGATC 3 cut(s) 183, 266, 335
Ama87I CYCGRG 2 cut(s) 343, 413
Aor13HI TCCGGA 2 cut(s) 255, 324
ApeKI GCWGC 5 cut(s) 267, 432, 487, 617, 815
AseI ATTAAT 1 cut(s) 596
AspLEI GCGC 2 cut(s) 487, 749
AspS9I GGNCC 3 cut(s) 31, 285, 643
AsuC2I CCSGG 5 cut(s) 6, 110, 156, 305, 647
AsuHPI GGTGA 4 cut(s) 58, 82, 253, 638
AsuII TTCGAA 1 cut(s) 776
AsuNHI GCTAGC 1 cut(s) 667
AvaI CYCGRG 2 cut(s) 343, 413
AvaII GGWCC 1 cut(s) 643
BanI GGYRCC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 70
BbvI GCAGC 5 cut(s) 279, 444, 474, 604, 827
BccI CCATC 5 cut(s) 26, 260, 605, 634, 773
BceAI ACGGC 4 cut(s) 45, 55, 59, 437
BciT130I CCWGG 1 cut(s) 94
BcnI CCSGG 5 cut(s) 6, 110, 156, 305, 647
BcoDI GTCTC 2 cut(s) 187, 346
BfaI CTAG 2 cut(s) 668, 855
Bme1390I CCNGG 6 cut(s) 6, 94, 110, 156, 305, 647
Bme18I GGWCC 1 cut(s) 643
BmeT110I CYCGRG 2 cut(s) 343, 413
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 3 cut(s) 31, 285, 643
BmiI GGNNCC 5 cut(s) 13, 98, 160, 645, 711
BmrFI CCNGG 6 cut(s) 6, 94, 110, 156, 305, 647
BmsI GCATC 4 cut(s) 51, 324, 369, 680
BmtI GCTAGC 1 cut(s) 671
BpiI GAAGAC 1 cut(s) 70
BpmI CTGGAG 1 cut(s) 205
Bpu14I TTCGAA 1 cut(s) 776
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 5 cut(s) 6, 110, 156, 305, 647
BsaHI GRCGYC 1 cut(s) 683
BsaJI CCNNGG 5 cut(s) 69, 154, 171, 235, 793
BsaWI WCCGGW 2 cut(s) 255, 324
BsaXI ACNNNNNCTCC 6 cut(s) 41, 71, 182, 212, 864, 894
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 247, 282, 324, 382
Bse118I RCCGGY 3 cut(s) 282, 362, 620
BseAI TCCGGA 2 cut(s) 255, 324
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 5 cut(s) 69, 154, 171, 235, 793
BseGI GGATG 4 cut(s) 18, 252, 339, 468
BseLI CCNNNNNNNGG 5 cut(s) 28, 247, 282, 324, 382
BseRI GAGGAG 1 cut(s) 311
BseX3I CGGCCG 1 cut(s) 360
BseXI GCAGC 5 cut(s) 279, 444, 474, 604, 827
BseYI CCCAGC 1 cut(s) 139
BsgI GTGCAG 2 cut(s) 422, 672
Bsh1236I CGCG 2 cut(s) 485, 747
Bsh1285I CGRYCG 1 cut(s) 363
BshNI GGYRCC 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 363
BsiHKCI CYCGRG 2 cut(s) 343, 413
BslFI GGGAC 3 cut(s) 430, 443, 461
BslI CCNNNNNNNGG 5 cut(s) 28, 247, 282, 324, 382
BsmAI GTCTC 2 cut(s) 187, 346
BsmBI CGTCTC 2 cut(s) 187, 346
BsmFI GGGAC 3 cut(s) 430, 443, 461
BsoBI CYCGRG 2 cut(s) 343, 413
Bsp119I TTCGAA 1 cut(s) 776
Bsp13I TCCGGA 2 cut(s) 255, 324
Bsp1407I TGTACA 1 cut(s) 660
Bsp143I GATC 4 cut(s) 175, 258, 327, 405
BspEI TCCGGA 2 cut(s) 255, 324
BspFNI CGCG 2 cut(s) 485, 747
BspLI GGNNCC 5 cut(s) 13, 98, 160, 645, 711
BspOI GCTAGC 1 cut(s) 671
BspPI GGATC 3 cut(s) 183, 266, 335
BspT104I TTCGAA 1 cut(s) 776
BspT107I GGYRCC 1 cut(s) 11
BsrBI CCGCTC 1 cut(s) 463
BsrFI RCCGGY 3 cut(s) 282, 362, 620
BsrGI TGTACA 1 cut(s) 660
BssAI RCCGGY 3 cut(s) 282, 362, 620
BssECI CCNNGG 5 cut(s) 69, 154, 171, 235, 793
BssMI GATC 4 cut(s) 175, 258, 327, 405
BssNI GRCGYC 1 cut(s) 683
BssT1I CCWWGG 2 cut(s) 69, 793
Bst2UI CCWGG 1 cut(s) 94
Bst6I CTCTTC 2 cut(s) 68, 221
BstACI GRCGYC 1 cut(s) 683
BstAPI GCANNNNNTGC 1 cut(s) 677
BstAUI TGTACA 1 cut(s) 660
BstBI TTCGAA 1 cut(s) 776
BstC8I GCNNGC 8 cut(s) 148, 249, 288, 384, 427, 622, 669, 888
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 4 cut(s) 18, 252, 339, 468
BstFNI CGCG 2 cut(s) 485, 747
BstHHI GCGC 2 cut(s) 487, 749
BstKTI GATC 4 cut(s) 178, 261, 330, 408
BstMAI GTCTC 2 cut(s) 187, 346
BstMBI GATC 4 cut(s) 175, 258, 327, 405
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 6 cut(s) 409, 677, 686, 744, 753, 867
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 6 cut(s) 4, 92, 108, 154, 303, 645
BstUI CGCG 2 cut(s) 485, 747
BstV1I GCAGC 5 cut(s) 279, 444, 474, 604, 827
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 360
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 4 cut(s) 18, 252, 339, 468
Cac8I GCNNGC 8 cut(s) 148, 249, 288, 384, 427, 622, 669, 888
CfoI GCGC 2 cut(s) 487, 749
Cfr10I RCCGGY 3 cut(s) 282, 362, 620
Cfr13I GGNCC 3 cut(s) 31, 285, 643
CseI GACGC 1 cut(s) 672
Csp6I GTAC 3 cut(s) 661, 743, 852
CviAII CATG 2 cut(s) 517, 891
CviQI GTAC 3 cut(s) 661, 743, 852
DpnI GATC 4 cut(s) 177, 260, 329, 407
DpnII GATC 4 cut(s) 175, 258, 327, 405
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
EaeI YGGCCR 2 cut(s) 72, 360
EagI CGGCCG 1 cut(s) 360
Eam1104I CTCTTC 2 cut(s) 68, 221
EarI CTCTTC 2 cut(s) 68, 221
EclXI CGGCCG 1 cut(s) 360
Eco130I CCWWGG 2 cut(s) 69, 793
Eco147I AGGCCT 1 cut(s) 805
Eco47I GGWCC 1 cut(s) 643
Eco52I CGGCCG 1 cut(s) 360
Eco57I CTGAAG 1 cut(s) 187
Eco88I CYCGRG 2 cut(s) 343, 413
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 92
EcoT14I CCWWGG 2 cut(s) 69, 793
ErhI CCWWGG 2 cut(s) 69, 793
Esp3I CGTCTC 2 cut(s) 187, 346
FaeI CATG 2 cut(s) 520, 894
FaiI YATR 3 cut(s) 498, 518, 892
FaqI GGGAC 3 cut(s) 430, 443, 461
FatI CATG 2 cut(s) 516, 890
FauI CCCGC 3 cut(s) 272, 295, 468
FblI GTMKAC 1 cut(s) 195
FokI GGATG 4 cut(s) 5, 239, 346, 455
FspBI CTAG 2 cut(s) 668, 855
GlaI GCGC 2 cut(s) 486, 748
GsaI CCCAGC 1 cut(s) 143
GsuI CTGGAG 1 cut(s) 205
HgaI GACGC 1 cut(s) 672
HhaI GCGC 2 cut(s) 487, 749
Hin1I GRCGYC 1 cut(s) 683
Hin1II CATG 2 cut(s) 520, 894
Hin6I GCGC 2 cut(s) 485, 747
HinP1I GCGC 2 cut(s) 485, 747
HincII GTYRAC 4 cut(s) 49, 55, 310, 394
HindII GTYRAC 4 cut(s) 49, 55, 310, 394
HinfI GANTC 2 cut(s) 295, 798
HphI GGTGA 4 cut(s) 58, 82, 253, 638
Hpy166II GTNNAC 6 cut(s) 49, 55, 196, 310, 394, 661
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 6 cut(s) 127, 256, 325, 331, 376, 415
Hpy8I GTNNAC 6 cut(s) 49, 55, 196, 310, 394, 661
Hpy99I CGWCG 7 cut(s) 41, 86, 137, 170, 233, 458, 485
HpyAV CCTTC 5 cut(s) 196, 211, 220, 515, 596
HpyCH4IV ACGT 7 cut(s) 51, 192, 396, 651, 702, 832, 850
HpyCH4V TGCA 5 cut(s) 403, 504, 689, 815, 837
HpyF10VI GCNNNNNNNGC 6 cut(s) 409, 677, 686, 744, 753, 867
HpySE526I ACGT 7 cut(s) 51, 192, 396, 651, 702, 832, 850
Hsp92I GRCGYC 1 cut(s) 683
Hsp92II CATG 2 cut(s) 520, 894
HspAI GCGC 2 cut(s) 485, 747
Kpn2I TCCGGA 2 cut(s) 255, 324
KroI GCCGGC 1 cut(s) 620
KroNI GCCGGC 1 cut(s) 622
Kzo9I GATC 4 cut(s) 175, 258, 327, 405
LmnI GCTCC 3 cut(s) 212, 767, 872
Lsp1109I GCAGC 5 cut(s) 279, 444, 474, 604, 827
LweI GCATC 4 cut(s) 51, 324, 369, 680
MaeI CTAG 2 cut(s) 668, 855
MaeII ACGT 7 cut(s) 51, 192, 396, 651, 702, 832, 850
MalI GATC 4 cut(s) 177, 260, 329, 407
MbiI CCGCTC 1 cut(s) 463
MboI GATC 4 cut(s) 175, 258, 327, 405
MboII GAAGA 3 cut(s) 55, 70, 208
MflI RGATCY 1 cut(s) 175
MluCI AATT 3 cut(s) 573, 586, 597
MmeI TCCRAC 1 cut(s) 129
MroI TCCGGA 2 cut(s) 255, 324
MroNI GCCGGC 1 cut(s) 620
MseI TTAA 1 cut(s) 596
MspA1I CMGCKG 1 cut(s) 143
MspR9I CCNGG 6 cut(s) 6, 94, 110, 156, 305, 647
MvaI CCWGG 1 cut(s) 94
MvnI CGCG 2 cut(s) 485, 747
MwoI GCNNNNNNNGC 6 cut(s) 409, 677, 686, 744, 753, 867
NaeI GCCGGC 1 cut(s) 622
NciI CCSGG 5 cut(s) 6, 110, 156, 305, 647
NdeII GATC 4 cut(s) 175, 258, 327, 405
NgoMIV GCCGGC 1 cut(s) 620
NheI GCTAGC 1 cut(s) 667
NlaIII CATG 2 cut(s) 520, 894
NlaIV GGNNCC 5 cut(s) 13, 98, 160, 645, 711
NmeAIII GCCGAG 3 cut(s) 196, 216, 316
NspV TTCGAA 1 cut(s) 776
PceI AGGCCT 1 cut(s) 805
PcsI WCGNNNNNNNCGW 7 cut(s) 42, 132, 336, 453, 462, 471, 680
PdiI GCCGGC 1 cut(s) 622
PfeI GAWTC 2 cut(s) 295, 798
PshBI ATTAAT 1 cut(s) 596
Psp1406I AACGTT 1 cut(s) 832
Psp6I CCWGG 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 139
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 5 cut(s) 13, 98, 160, 645, 711
PspPI GGNCC 3 cut(s) 31, 285, 643
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 3 cut(s) 662, 744, 853
RsaNI GTAC 3 cut(s) 661, 743, 852
SaqAI TTAA 1 cut(s) 596
Sau3AI GATC 4 cut(s) 175, 258, 327, 405
Sau96I GGNCC 3 cut(s) 31, 285, 643
ScrFI CCNGG 6 cut(s) 6, 94, 110, 156, 305, 647
SfaNI GCATC 4 cut(s) 51, 324, 369, 680
SfuI TTCGAA 1 cut(s) 776
SinI GGWCC 1 cut(s) 643
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 3 cut(s) 573, 586, 597
SseBI AGGCCT 1 cut(s) 805
SspMI CTAG 2 cut(s) 668, 855
StuI AGGCCT 1 cut(s) 805
StyD4I CCNGG 6 cut(s) 4, 92, 108, 154, 303, 645
StyI CCWWGG 2 cut(s) 69, 793
TaiI ACGT 7 cut(s) 54, 195, 399, 654, 705, 835, 853
TaqI TCGA 3 cut(s) 36, 126, 776
TasI AATT 3 cut(s) 573, 586, 597
TatI WGTACW 1 cut(s) 660
TauI GCSGC 7 cut(s) 12, 146, 153, 362, 432, 452, 683
TfiI GAWTC 2 cut(s) 295, 798
Tru1I TTAA 1 cut(s) 596
Tru9I TTAA 1 cut(s) 596
TseI GCWGC 5 cut(s) 267, 432, 487, 617, 815
TspGWI ACGGA 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 643
VspI ATTAAT 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 2 cut(s) 668, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.