Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007059

Physical Location & Seq

Genomic Coordinates Forward (+)

26178579 .. 26182792

Gene Structure 4214 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1153400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3246 bp

ATGCCTACGCCGGTTACTGTGGCCAGGCAATGCCTGACAGCGGAGGCAGCAAACGCTTTGGACGAGGCCGTGGCAGTCGCGCGCCGTAGAGGACACGGCCAAACGACGTCACTGCACGCCATATCCGCTCTGCTCTCCCTCCCGTCCTCCGCGCTACGAGACGCCTGCGCGCGAGCCCGAAACTCCGCCTACCCGCCCCGACTCCAATTCAAGGCTCTGGAGCTCTGCCTCAGCGTCTCGCTCGACCGGGTCTCCTCGACCCAACTCGCCGACGACCCGCCCGTTTCGAACTCGCTCATGGCCGCCATCAAACGGTCACAAGCCAACCAGCGAAGGCAGCCCGAGAATTACCACCTCTACCACCAGCTCTCGCAGCAGTCCTCGATCTCCGCCATTAAAGTGGAGCTCCAGCACCTGATTCTATCGATTCTCGACGACCCCGTCGTCAGTCGGGTCTTCGCCGAAGCGGGTTTTCGTAGCTCCGAAATAAAGCTCGCAATTTTGCGCCCTTTTCCTCAGATTCTCAGATACCCGCGCTCCAGAGGCCACCATCCTCTCTTTCTCTGCAACTTAGCCGAGTACCCTGACACGGGTCACCCGACCCGATCCGTTTTAACTGACGGGGACGAAAACTCGAGGAGAATAGGAGAGGTTCTCGGGAGAAACAGAGGGCGGAATCCTCTGCTTGTGGGTGTATACGCTCATGACGCACTCAAGAGCTTCGTGGAGGCTCTGGAGAAAAAAGACGGCGGCGTTTTGCCGGCGGAGCTATCTGGGTTGTCTGTAGTTTCAGCGGAAAACGATGTGTCAAAGTTCATAGCCGAGGATTACGATAAAGGGTCAGTAAGTTTGAGGTTCGGGGAGGTGGGTCGGGTCGCAGAGCAGAGTTTGGGGCCTGGGATTGTTGTGAATATTGGGGACTTGAAGGCGTTTGTGGCTGAGAACGCGGTGGCTGACTCTGTGAGTCACGTGGTTGCCGAGGTCACAAGGCTGTTGGAGGTTCAGCGCGGGAAAGTTTGGTTGATTGGTGCAACGGCTAGCTATGGAAGTTACTTGAAGTTTGTGGGGAGATTTCCATCTGTGGAAAAAGACTGGGACTTGCAGCTTCTGCCCATCACTTCTCTCAGAGCTGCTTCCATGGCTGAATCATATCCCAGGTCCAGCTTGATGGAGTCGTTTGTTCCATTTGGTGGATTCTTTTCAGCACCTTCTGACCTAAAGCTCCCAATAAGTTGCTCCTACCAATGTCTTCCCCGCAATCACCAGCGCAATAAAATATCCGAGCAAGAAGCATGTTCAGTTCCAATGGGAGGTATTACCGCTTCAGTGGCAGGTCAGCCCCAAGCTAGTTTGCCTTCTTGGTTGCAGATGGCTCCGCTTTGCACAAACAAGGGATCGGATGTGAAGACCAAAGATGATGGTGTGTTATCGAGTGCCAAAGTTTCAGGATTGCAAAAGAAATGGGATGATAAATGCCAGCATCTTCACTACTCTCATCCCCTGCCCGAAGCAAACTTCTTTCCAACTATGGTAGGATTTCAGTCTCCTGTGGAGAAGTGCAACCACGACAACACCACCAATATATCTTCACAGAAAATTGAGTGCAAGATTGCAGATTCATGCATGCCTGCAGACGTGCAAACACAGTCAAGCCTTCCTCCAAAGGCTAAGAACGACAGTTTCTCTTCCGAAGTGTGGGAAAAAACTTCAAAAGATGAAGATCTTGAGTCCGCTGGCCTCAGGTCACCTTGCTTGTCCAACTCTACTGTGGTTGATGGTAGCCAAACATCTGCCACATCAGCTGCTTCCGTCACCACAGATTTAGGGTTAGGAATATGCTCTTCTCCTGCTAGTAATACGCCGAAAAAACCTCCAGATCTGAATCCAGCAGTTCAACAGGACATCTTGGGTTGCTTTTCCTCTAATATTGATTTAGTCAATGGGAATCTCTATTCTACTCGATCATCATCCTCCTCGAGTCCTGACAACCATGGGCAGTTTGATCCAAGTGATGTCAAGATGCTTTTCAGAGCTCTCTTTGAAAGGGTTGGCTGGCAAACTGATGCTGTAAGTGTAATTAGCAGAAGAATAGCTAACTGCCGATCTAGAAGTGCAAAATTCTGTGGAGCAAGTCACAGAAGGGATGTATGGTTCAATTTCACAGGACCTGATAGGTATGGTAAAAAGAAAATTGCCCTTGCCCTTGCTGAGGTATTATACGGAAACCAGGAACAATTGATCTGTGCGGATCTAAATTCTCAGGATGGAATGATCCATTCAGACACAAACTTTGATTGTCCGGTTGTGAATGGTTATGATGTGAGGTTTAGAGGTAAGACGGTTATTGATTATGTTGCCGGAGAATTGTGCAAGAAACCATTGTCCATTATCTTCTTAGAAAATGTAGATAAGGCTGATGTGGTGACTCAAAATAGTTTGTCCTTGGCTTTATTGACCGGTAAATTTTCCGATTCCCATGGAAGACAAGTCAGCACCAATAATGCGATATTTGTTACAACTTCGACATTCTCGAAGGGATGCAGTATTCTCAGTTCCACGAAGGGACTCTCCAACTATTCCGAGGAAAGAATCTTACAAGCGAAAGAGCGGCCTGTGCAGATTACTATTGAATGTGCCTCCGAAGACAGCAGCAGAAGCCAAAACTGGAGGGCATCCACGAATCAACATTTTCTAAATAAAAGGAAACTGGTCGGTGTCAATGAACTTCTAGGGCAGCATGAAGTCTCAGAGATGCCCAAACGGGCCAACAAGACGTCAACTAGGTATCTGGATCTCAACCTTCCAGCTGAAGAGAATGCAGTGGAAAACACAGACGATGGGAGCTCTGAAAACGACTACCTATCTGAAAACTCCAAGTCCTCGTTGCAAGACTTCCTTGATCAGGTGCACGAGACAGTGGTATTCAAGCCCGTTGATTTTGATGCACTTGCTGAGAAAATATCAAAGGAAATCAAGAATAGCTTCCACAAGTTTGTTGACTCAGAGTGCCTGCTAGAAATCGATTCAGAAGTCGTGGAACAACTACTTGCAGCTGTATATTTGTCAGACAGGTCGAGGGTGGTGGAAGATTGGGTGGAACAAGTACTCAGCAGGGCATTTGCAGAAGTTCAAAAGCGACATAACTCGCACAGAATCACTACCGTAAAACTCAAAACTTGCGGAGGGATTTGGTTGGAACACCGAGCACCAAAAACTTATCTTGCCCCTAGCATTGTACTAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_2 Clp_N

Protein Analysis

1082

Amino Acids

118.54

Weight (kDa)

6.46

Isoelectric Point (pI)

50.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NBD_SMAX1	PF23569	211 - 375	5.1e-20	SMAX1 nucleotide binding domain
AAA_2	PF07724	717 - 845	2.3e-10	AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1	PF26587	979 - 1062	8.5e-31	SMAX1-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007059)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40130 AT2G40130
fragaria_vesca	FvH4_7g24540 FvH4_7g24540
malus_domestica	MD01G1153400.v1.1 MD07G1222400.v1.1
prunus_persica	Prupe.2G250700_v2.0.a1 Prupe.2G250700_v2.0.a1
pyrus_communis	pycom01g17100 pycom07g20100 pycom07g20110
rosa_chinensis	RchiOBHm_Chr1g0370371
rosa_laevigata	RLG00000027054
rosa_multiflora	Rmu_co8224134.1_g000001 Rmu_co8315027.1_g000001 Rmu_sc0006707.1_g000030 Rmu_sc0029129.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286640
rosa_rugosa	Rorug01G0355800 Rorug01G0355900
rosa_samantha	Rh1AG364200 Rh1BG327500 Rh1CG340500 Rh1DG357800
rosa_wichuraiana	Rw1G032050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	440, 443
AatII	GACGTC	2 cut(s)	110, 2777
Acc36I	ACCTGC	1 cut(s)	1322
AccB7I	CCANNNNNTGG	1 cut(s)	1190
AccBSI	CCGCTC	2 cut(s)	128, 2608
AccI	GTMKAC	1 cut(s)	696
AccII	CGCG	8 cut(s)	80, 82, 152, 170, 172, 535, 947, 1008
AclWI	GGATC	6 cut(s)	600, 1402, 1997, 2256, 2266, 2799
AcoI	YGGCCR	3 cut(s)	21, 97, 300
AcsI	RAATTY	3 cut(s)	2117, 2254, 2462
AcuI	CTGAAG	2 cut(s)	1308, 2829
AcvI	CACGTG	1 cut(s)	970
AcyI	GRCGYC	3 cut(s)	107, 162, 2774
AfaI	GTAC	3 cut(s)	581, 3105, 3237
AgeI	ACCGGT	1 cut(s)	2456
AjiI	CACGTC	1 cut(s)	1636
AjnI	CCWGG	4 cut(s)	23, 895, 1154, 2226
AloI	GAACNNNNNNTCC	2 cut(s)	2135, 2167
Alw21I	GWGCWC	6 cut(s)	225, 408, 2035, 2846, 2910, 3208
Alw26I	GTCTC	6 cut(s)	153, 241, 256, 1548, 2749, 2906
Alw44I	GTGCAC	1 cut(s)	2906
AlwI	GGATC	6 cut(s)	600, 1402, 1997, 2256, 2266, 2799
AlwNI	CAGNNNCTG	3 cut(s)	415, 1108, 2168
Ama87I	CYCGRG	4 cut(s)	341, 634, 656, 1975
AoxI	GGCC	9 cut(s)	21, 66, 97, 300, 544, 893, 1735, 2609, 2763
ApaLI	GTGCAC	1 cut(s)	2906
ApeKI	GCWGC	9 cut(s)	47, 337, 373, 1102, 1130, 1802, 2649, 2734, 3050
ApoI	RAATTY	3 cut(s)	2117, 2254, 2462
ArsI	GACNNNNNNTTYG	2 cut(s)	2273, 2305
AsiGI	ACCGGT	1 cut(s)	2456
Asp700I	GAANNNNTTC	1 cut(s)	2981
AspLEI	GCGC	9 cut(s)	82, 84, 154, 170, 172, 507, 537, 1008, 1269
AspS9I	GGNCC	4 cut(s)	893, 1158, 2165, 2763
AsuC2I	CCSGG	1 cut(s)	248
AsuHPI	GGTGA	5 cut(s)	587, 1253, 1737, 1804, 2434
AsuII	TTCGAA	1 cut(s)	287
AsuNHI	GCTAGC	1 cut(s)	1037
AvaI	CYCGRG	4 cut(s)	341, 634, 656, 1975
AvaII	GGWCC	2 cut(s)	1158, 2165
AxyI	CCTNAGG	1 cut(s)	1739
BaeGI	GKGCMC	1 cut(s)	2910
BalI	TGGCCA	1 cut(s)	23
BanII	GRGCYC	5 cut(s)	178, 225, 408, 2035, 2846
BarI	GAAGNNNNNNTAC	2 cut(s)	1306, 1338
BauI	CACGAG	1 cut(s)	2909
BbrPI	CACGTG	1 cut(s)	970
BbsI	GAAGAC	5 cut(s)	448, 1241, 1412, 2488, 2649
Bbv12I	GWGCWC	6 cut(s)	225, 408, 2035, 2846, 2910, 3208
BbvCI	CCTCAGC	2 cut(s)	230, 2208
BbvI	GCAGC	9 cut(s)	59, 349, 385, 1114, 1117, 1789, 2661, 2746, 3062
BceAI	ACGGC	5 cut(s)	53, 69, 112, 763, 1050
BcgI	CGANNNNNNTGC	4 cut(s)	94, 128, 147, 181
BciT130I	CCWGG	4 cut(s)	25, 897, 1156, 2228
BclI	TGATCA	1 cut(s)	2899
BcnI	CCSGG	1 cut(s)	248
BcoDI	GTCTC	6 cut(s)	153, 241, 256, 1548, 2749, 2906
BfaI	CTAG	8 cut(s)	1038, 1347, 1851, 2106, 2729, 2781, 3014, 3228
BfmI	CTRYAG	2 cut(s)	783, 1629
BfuAI	ACCTGC	1 cut(s)	1322
BglII	AGATCT	2 cut(s)	1720, 1876
BmcAI	AGTACT	1 cut(s)	3105
Bme1390I	CCNGG	5 cut(s)	25, 248, 897, 1156, 2228
Bme18I	GGWCC	2 cut(s)	1158, 2165
BmeT110I	CYCGRG	4 cut(s)	341, 634, 656, 1975
BmgBI	CACGTC	1 cut(s)	1636
BmgT120I	GGNCC	4 cut(s)	893, 1158, 2165, 2763
BmiI	GGNNCC	2 cut(s)	894, 1374
BmrFI	CCNGG	5 cut(s)	25, 248, 897, 1156, 2228
BmrI	ACTGGG	1 cut(s)	1102
BmsI	GCATC	7 cut(s)	1489, 2010, 2053, 2528, 2681, 2742, 2932
BmtI	GCTAGC	1 cut(s)	1041
BmuI	ACTGGG	1 cut(s)	1102
BoxI	GACNNNNGTC	1 cut(s)	591
BpiI	GAAGAC	5 cut(s)	448, 1241, 1412, 2488, 2649
BplI	GAGNNNNNCTC	2 cut(s)	639, 671
BpmI	CTGGAG	6 cut(s)	239, 392, 523, 755, 1857, 2686
Bpu10I	CCTNAGC	2 cut(s)	230, 2208
Bpu14I	TTCGAA	1 cut(s)	287
BpuEI	CTTGAG	2 cut(s)	698, 1745
BpuMI	CCSGG	1 cut(s)	248
Bsa29I	ATCGAT	2 cut(s)	425, 3021
BsaAI	YACGTR	1 cut(s)	970
BsaBI	GATNNNNATC	4 cut(s)	1075, 1719, 1881, 1966
BsaHI	GRCGYC	3 cut(s)	107, 162, 2774
BsaI	GGTCTC	1 cut(s)	256
BsaWI	WCCGGW	2 cut(s)	2299, 2456
BsaXI	ACNNNNNCTCC	4 cut(s)	236, 266, 521, 551
Bse118I	RCCGGY	3 cut(s)	10, 760, 2456
Bse1I	ACTGG	3 cut(s)	1097, 2669, 2712
Bse21I	CCTNAGG	1 cut(s)	1739
Bse3DI	GCAATG	1 cut(s)	35
Bse8I	GATNNNNATC	4 cut(s)	1075, 1719, 1881, 1966
BseBI	CCWGG	4 cut(s)	25, 897, 1156, 2228
BseCI	ATCGAT	2 cut(s)	425, 3021
BseGI	GGATG	9 cut(s)	550, 1405, 1471, 1495, 1967, 2149, 2269, 2543, 2672
BseJI	GATNNNNATC	4 cut(s)	1075, 1719, 1881, 1966
BseMI	GCAATG	1 cut(s)	35
BseNI	ACTGG	3 cut(s)	1097, 2669, 2712
BsePI	GCGCGC	2 cut(s)	80, 168
BseRI	GAGGAG	3 cut(s)	244, 652, 1963
BseSI	GKGCMC	1 cut(s)	2910
BseXI	GCAGC	9 cut(s)	59, 349, 385, 1114, 1117, 1789, 2661, 2746, 3062
BsgI	GTGCAG	2 cut(s)	98, 2636
Bsh1236I	CGCG	8 cut(s)	80, 82, 152, 170, 172, 535, 947, 1008
Bsh1285I	CGRYCG	1 cut(s)	247
BshFI	GGCC	9 cut(s)	23, 68, 99, 302, 546, 895, 1737, 2611, 2765
BshTI	ACCGGT	1 cut(s)	2456
BshVI	ATCGAT	2 cut(s)	425, 3021
BsiEI	CGRYCG	1 cut(s)	247
BsiHKAI	GWGCWC	6 cut(s)	225, 408, 2035, 2846, 2910, 3208
BsiHKCI	CYCGRG	4 cut(s)	341, 634, 656, 1975
BsiSI	CCGG	6 cut(s)	11, 247, 761, 2300, 2358, 2457
BslFI	GGGAC	4 cut(s)	638, 932, 1109, 2577
BsmAI	GTCTC	6 cut(s)	153, 241, 256, 1548, 2749, 2906
BsmBI	CGTCTC	2 cut(s)	153, 241
BsmFI	GGGAC	4 cut(s)	638, 932, 1109, 2577
BsmI	GAATGC	1 cut(s)	2821
BsnI	GGCC	9 cut(s)	23, 68, 99, 302, 546, 895, 1737, 2611, 2765
Bso31I	GGTCTC	1 cut(s)	256
BsoBI	CYCGRG	4 cut(s)	341, 634, 656, 1975
Bsp119I	TTCGAA	1 cut(s)	287
Bsp1286I	GDGCHC	7 cut(s)	178, 225, 408, 2035, 2846, 2910, 3208
Bsp19I	CCATGG	3 cut(s)	1137, 1990, 2476
BspANI	GGCC	9 cut(s)	23, 68, 99, 302, 546, 895, 1737, 2611, 2765
BspDI	ATCGAT	2 cut(s)	425, 3021
BspFNI	CGCG	8 cut(s)	80, 82, 152, 170, 172, 535, 947, 1008
BspHI	TCATGA	1 cut(s)	703
BspLI	GGNNCC	2 cut(s)	894, 1374
BspMAI	CTGCAG	1 cut(s)	1633
BspMI	ACCTGC	1 cut(s)	1322
BspOI	GCTAGC	1 cut(s)	1041
BspPI	GGATC	6 cut(s)	600, 1402, 1997, 2256, 2266, 2799
BspQI	GCTCTTC	1 cut(s)	1846
BspT104I	TTCGAA	1 cut(s)	287
BspTNI	GGTCTC	1 cut(s)	256
BsrBI	CCGCTC	2 cut(s)	128, 2608
BsrDI	GCAATG	1 cut(s)	35
BsrFI	RCCGGY	3 cut(s)	10, 760, 2456
BsrI	ACTGG	3 cut(s)	1097, 2669, 2712
BssAI	RCCGGY	3 cut(s)	10, 760, 2456
BssHII	GCGCGC	2 cut(s)	80, 168
BssNAI	GTATAC	1 cut(s)	697
BssNI	GRCGYC	3 cut(s)	107, 162, 2774
BssSI	CACGAG	1 cut(s)	2909
BssT1I	CCWWGG	4 cut(s)	1137, 1990, 2442, 2476
Bst1107I	GTATAC	1 cut(s)	697
Bst2BI	CACGAG	1 cut(s)	2909
Bst2UI	CCWGG	4 cut(s)	25, 897, 1156, 2228
Bst4CI	ACNGT	8 cut(s)	19, 315, 1647, 1679, 1768, 2341, 2917, 3163
Bst6I	CTCTTC	3 cut(s)	1690, 1846, 2805
BstACI	GRCGYC	3 cut(s)	107, 162, 2774
BstAPI	GCANNNNNTGC	1 cut(s)	1108
BstBAI	YACGTR	1 cut(s)	970
BstBI	TTCGAA	1 cut(s)	287
BstDSI	CCRYGG	4 cut(s)	69, 1137, 1990, 2476
BstEII	GGTNACC	2 cut(s)	593, 1743
BstENI	CCTNNNNNAGG	2 cut(s)	2207, 2900
BstF5I	GGATG	9 cut(s)	550, 1405, 1471, 1495, 1967, 2149, 2269, 2543, 2672
BstFNI	CGCG	8 cut(s)	80, 82, 152, 170, 172, 535, 947, 1008
BstHHI	GCGC	9 cut(s)	82, 84, 154, 170, 172, 507, 537, 1008, 1269
BstMAI	GTCTC	6 cut(s)	153, 241, 256, 1548, 2749, 2906
BstMCI	CGRYCG	1 cut(s)	247
BstNI	CCWGG	4 cut(s)	25, 897, 1156, 2228
BstNSI	RCATGY	2 cut(s)	1296, 1627
BstPAI	GACNNNNGTC	1 cut(s)	591
BstPI	GGTNACC	2 cut(s)	593, 1743
BstSCI	CCNGG	5 cut(s)	23, 246, 895, 1154, 2226
BstSFI	CTRYAG	2 cut(s)	783, 1629
BstSLI	GKGCMC	1 cut(s)	2910
BstUI	CGCG	8 cut(s)	80, 82, 152, 170, 172, 535, 947, 1008
BstV1I	GCAGC	9 cut(s)	59, 349, 385, 1114, 1117, 1789, 2661, 2746, 3062
BstV2I	GAAGAC	5 cut(s)	448, 1241, 1412, 2488, 2649
BstX2I	RGATCY	4 cut(s)	1720, 1876, 2248, 2791
BstXI	CCANNNNNNTGG	2 cut(s)	400, 1168
BstYI	RGATCY	4 cut(s)	1720, 1876, 2248, 2791
BstZ17I	GTATAC	1 cut(s)	697
Bsu15I	ATCGAT	2 cut(s)	425, 3021
Bsu36I	CCTNAGG	1 cut(s)	1739
BsuRI	GGCC	9 cut(s)	23, 68, 99, 302, 546, 895, 1737, 2611, 2765
BsuTUI	ATCGAT	2 cut(s)	425, 3021
BtgI	CCRYGG	4 cut(s)	69, 1137, 1990, 2476
BtrI	CACGTC	1 cut(s)	1636
BtsCI	GGATG	9 cut(s)	550, 1405, 1471, 1495, 1967, 2149, 2269, 2543, 2672
BtsI	GCAGTG	2 cut(s)	110, 2826
BtsIMutI	CAGTG	4 cut(s)	110, 1332, 2826, 2922
BveI	ACCTGC	1 cut(s)	1322
CaiI	CAGNNNCTG	3 cut(s)	415, 1108, 2168
CciI	TCATGA	1 cut(s)	703
CfoI	GCGC	9 cut(s)	82, 84, 154, 170, 172, 507, 537, 1008, 1269
Cfr10I	RCCGGY	3 cut(s)	10, 760, 2456
Cfr13I	GGNCC	4 cut(s)	893, 1158, 2165, 2763
ClaI	ATCGAT	2 cut(s)	425, 3021
CseI	GACGC	3 cut(s)	170, 223, 716
Csp6I	GTAC	3 cut(s)	580, 3104, 3236
CspAI	ACCGGT	1 cut(s)	2456
CviAII	CATG	9 cut(s)	298, 704, 1138, 1293, 1620, 1624, 1991, 2477, 2738
CviQI	GTAC	3 cut(s)	580, 3104, 3236
DrdI	GACNNNNNNGTC	2 cut(s)	440, 443
DseDI	GACNNNNNNGTC	2 cut(s)	440, 443
EaeI	YGGCCR	3 cut(s)	21, 97, 300
Eam1104I	CTCTTC	3 cut(s)	1690, 1846, 2805
EarI	CTCTTC	3 cut(s)	1690, 1846, 2805
EciI	GGCGGA	4 cut(s)	175, 379, 688, 779
Ecl136II	GAGCTC	4 cut(s)	223, 406, 2033, 2844
Eco130I	CCWWGG	4 cut(s)	1137, 1990, 2442, 2476
Eco24I	GRGCYC	5 cut(s)	178, 225, 408, 2035, 2846
Eco31I	GGTCTC	1 cut(s)	256
Eco47I	GGWCC	2 cut(s)	1158, 2165
Eco53kI	GAGCTC	4 cut(s)	223, 406, 2033, 2844
Eco57I	CTGAAG	2 cut(s)	1308, 2829
Eco72I	CACGTG	1 cut(s)	970
Eco81I	CCTNAGG	1 cut(s)	1739
Eco88I	CYCGRG	4 cut(s)	341, 634, 656, 1975
Eco91I	GGTNACC	2 cut(s)	593, 1743
EcoICRI	GAGCTC	4 cut(s)	223, 406, 2033, 2844
EcoNI	CCTNNNNNAGG	2 cut(s)	2207, 2900
EcoO109I	RGGNCCY	2 cut(s)	893, 2165
EcoO65I	GGTNACC	2 cut(s)	593, 1743
EcoRII	CCWGG	4 cut(s)	23, 895, 1154, 2226
EcoT14I	CCWWGG	4 cut(s)	1137, 1990, 2442, 2476
EcoT22I	ATGCAT	1 cut(s)	1625
EcoT38I	GRGCYC	5 cut(s)	178, 225, 408, 2035, 2846
ErhI	CCWWGG	4 cut(s)	1137, 1990, 2442, 2476
Esp3I	CGTCTC	2 cut(s)	153, 241
FaeI	CATG	9 cut(s)	301, 707, 1141, 1296, 1623, 1627, 1994, 2480, 2741
FalI	AAGNNNNNCTT	4 cut(s)	2880, 2912, 2966, 2998
FaqI	GGGAC	4 cut(s)	638, 932, 1109, 2577
FatI	CATG	9 cut(s)	297, 703, 1137, 1292, 1619, 1623, 1990, 2476, 2737
FauI	CCCGC	6 cut(s)	201, 285, 460, 540, 1001, 1262
FbaI	TGATCA	1 cut(s)	2899
FblI	GTMKAC	1 cut(s)	696
FokI	GGATG	9 cut(s)	537, 1412, 1478, 1482, 1954, 2156, 2276, 2550, 2659
FriOI	GRGCYC	5 cut(s)	178, 225, 408, 2035, 2846
FspBI	CTAG	8 cut(s)	1038, 1347, 1851, 2106, 2729, 2781, 3014, 3228
GlaI	GCGC	9 cut(s)	81, 83, 153, 169, 171, 506, 536, 1007, 1268
GsuI	CTGGAG	6 cut(s)	239, 392, 523, 755, 1857, 2686
HaeIII	GGCC	9 cut(s)	23, 68, 99, 302, 546, 895, 1737, 2611, 2765
HapII	CCGG	6 cut(s)	11, 247, 761, 2300, 2358, 2457
HgaI	GACGC	3 cut(s)	170, 223, 716
HhaI	GCGC	9 cut(s)	82, 84, 154, 170, 172, 507, 537, 1008, 1269
Hin1I	GRCGYC	3 cut(s)	107, 162, 2774
Hin1II	CATG	9 cut(s)	301, 707, 1141, 1296, 1623, 1627, 1994, 2480, 2741
Hin6I	GCGC	9 cut(s)	80, 82, 152, 168, 170, 505, 535, 1006, 1267
HinP1I	GCGC	9 cut(s)	80, 82, 152, 168, 170, 505, 535, 1006, 1267
HincII	GTYRAC	2 cut(s)	2778, 2998
HindII	GTYRAC	2 cut(s)	2778, 2998
HpaII	CCGG	6 cut(s)	11, 247, 761, 2300, 2358, 2457
HphI	GGTGA	5 cut(s)	587, 1253, 1737, 1804, 2434
Hpy166II	GTNNAC	4 cut(s)	697, 2778, 2908, 2998
Hpy8I	GTNNAC	4 cut(s)	697, 2778, 2908, 2998
Hpy99I	CGWCG	4 cut(s)	109, 275, 437, 446
HpyAV	CCTTC	9 cut(s)	327, 919, 1218, 1365, 1664, 2133, 2527, 2554, 2810
HpyCH4III	ACNGT	8 cut(s)	19, 315, 1647, 1679, 1768, 2341, 2917, 3163
HpyCH4IV	ACGT	4 cut(s)	107, 969, 1635, 2774
HpySE526I	ACGT	4 cut(s)	107, 969, 1635, 2774
Hsp92I	GRCGYC	3 cut(s)	107, 162, 2774
Hsp92II	CATG	9 cut(s)	301, 707, 1141, 1296, 1623, 1627, 1994, 2480, 2741
HspAI	GCGC	9 cut(s)	80, 82, 152, 168, 170, 505, 535, 1006, 1267
KroI	GCCGGC	1 cut(s)	760
KroNI	GCCGGC	1 cut(s)	762
Ksp22I	TGATCA	1 cut(s)	2899
LguI	GCTCTTC	1 cut(s)	1846
Lsp1109I	GCAGC	9 cut(s)	59, 349, 385, 1114, 1117, 1789, 2661, 2746, 3062
LweI	GCATC	7 cut(s)	1489, 2010, 2053, 2528, 2681, 2742, 2932
MaeI	CTAG	8 cut(s)	1038, 1347, 1851, 2106, 2729, 2781, 3014, 3228
MaeII	ACGT	4 cut(s)	107, 969, 1635, 2774
MbiI	CCGCTC	2 cut(s)	128, 2608
MfeI	CAATTG	1 cut(s)	2234
MflI	RGATCY	4 cut(s)	1720, 1876, 2248, 2791
MhlI	GDGCHC	7 cut(s)	178, 225, 408, 2035, 2846, 2910, 3208
MlsI	TGGCCA	1 cut(s)	23
MluNI	TGGCCA	1 cut(s)	23
MlyI	GAGTC	9 cut(s)	195, 950, 973, 1181, 1736, 1987, 2419, 2559, 2993
MmeI	TCCRAC	5 cut(s)	975, 1547, 1782, 2595, 3174
Mox20I	TGGCCA	1 cut(s)	23
Mph1103I	ATGCAT	1 cut(s)	1625
MroNI	GCCGGC	1 cut(s)	760
MroXI	GAANNNNTTC	1 cut(s)	2981
MscI	TGGCCA	1 cut(s)	23
MseI	TTAA	2 cut(s)	396, 614
MslI	CAYNNNNRTG	1 cut(s)	398
Msp20I	TGGCCA	1 cut(s)	23
MspA1I	CMGCKG	6 cut(s)	41, 794, 1733, 1802, 2807, 3053
MspI	CCGG	6 cut(s)	11, 247, 761, 2300, 2358, 2457
MspR9I	CCNGG	5 cut(s)	25, 248, 897, 1156, 2228
MunI	CAATTG	1 cut(s)	2234
Mva1269I	GAATGC	1 cut(s)	2821
MvaI	CCWGG	4 cut(s)	25, 897, 1156, 2228
MvnI	CGCG	8 cut(s)	80, 82, 152, 170, 172, 535, 947, 1008
NaeI	GCCGGC	1 cut(s)	762
NciI	CCSGG	1 cut(s)	248
NcoI	CCATGG	3 cut(s)	1137, 1990, 2476
NgoMIV	GCCGGC	1 cut(s)	760
NheI	GCTAGC	1 cut(s)	1037
NlaIII	CATG	9 cut(s)	301, 707, 1141, 1296, 1623, 1627, 1994, 2480, 2741
NlaIV	GGNNCC	2 cut(s)	894, 1374
NmeAIII	GCCGAG	3 cut(s)	601, 847, 1003
NmuCI	GTSAC	9 cut(s)	108, 315, 593, 965, 982, 1743, 1810, 2132, 2422
NsiI	ATGCAT	1 cut(s)	1625
NspI	RCATGY	2 cut(s)	1296, 1627
NspV	TTCGAA	1 cut(s)	287
PaeI	GCATGC	1 cut(s)	1627
PaeR7I	CTCGAG	2 cut(s)	634, 1975
PagI	TCATGA	1 cut(s)	703
PauI	GCGCGC	2 cut(s)	80, 168
PciSI	GCTCTTC	1 cut(s)	1846
PcsI	WCGNNNNNNNCGW	7 cut(s)	60, 279, 438, 441, 596, 975, 2528
PctI	GAATGC	1 cut(s)	2821
PdiI	GCCGGC	1 cut(s)	762
PdmI	GAANNNNTTC	1 cut(s)	2981
PflFI	GACNNNGTC	2 cut(s)	248, 440
PflMI	CCANNNNNTGG	1 cut(s)	1190
PinAI	ACCGGT	1 cut(s)	2456
PleI	GAGTC	9 cut(s)	195, 950, 972, 1180, 1735, 1986, 2419, 2559, 2993
PmaCI	CACGTG	1 cut(s)	970
PmlI	CACGTG	1 cut(s)	970
PpsI	GAGTC	9 cut(s)	195, 950, 972, 1180, 1735, 1986, 2419, 2559, 2993
Ppu21I	YACGTR	1 cut(s)	970
PpuMI	RGGWCCY	1 cut(s)	2165
PshAI	GACNNNNGTC	1 cut(s)	591
Psp124BI	GAGCTC	4 cut(s)	225, 408, 2035, 2846
Psp5II	RGGWCCY	1 cut(s)	2165
Psp6I	CCWGG	4 cut(s)	23, 895, 1154, 2226
PspCI	CACGTG	1 cut(s)	970
PspEI	GGTNACC	2 cut(s)	593, 1743
PspGI	CCWGG	4 cut(s)	23, 895, 1154, 2226
PspN4I	GGNNCC	2 cut(s)	894, 1374
PspPI	GGNCC	4 cut(s)	893, 1158, 2165, 2763
PspPPI	RGGWCCY	1 cut(s)	2165
PspXI	VCTCGAGB	2 cut(s)	634, 1975
PsrI	GAACNNNNNNTAC	2 cut(s)	2536, 2568
PstI	CTGCAG	1 cut(s)	1633
PstNI	CAGNNNCTG	3 cut(s)	415, 1108, 2168
PsuI	RGATCY	4 cut(s)	1720, 1876, 2248, 2791
PsyI	GACNNNGTC	2 cut(s)	248, 440
PteI	GCGCGC	2 cut(s)	80, 168
PvuII	CAGCTG	3 cut(s)	1802, 2807, 3053
RsaI	GTAC	3 cut(s)	581, 3105, 3237
RsaNI	GTAC	3 cut(s)	580, 3104, 3236
RseI	CAYNNNNRTG	1 cut(s)	398
SacI	GAGCTC	4 cut(s)	225, 408, 2035, 2846
SapI	GCTCTTC	1 cut(s)	1846
SaqAI	TTAA	2 cut(s)	396, 614
Sau96I	GGNCC	4 cut(s)	893, 1158, 2165, 2763
ScaI	AGTACT	1 cut(s)	3105
SchI	GAGTC	9 cut(s)	195, 950, 973, 1181, 1736, 1987, 2419, 2559, 2993
ScrFI	CCNGG	5 cut(s)	25, 248, 897, 1156, 2228
SduI	GDGCHC	7 cut(s)	178, 225, 408, 2035, 2846, 2910, 3208
SfaNI	GCATC	7 cut(s)	1489, 2010, 2053, 2528, 2681, 2742, 2932
SfcI	CTRYAG	2 cut(s)	783, 1629
Sfr274I	CTCGAG	2 cut(s)	634, 1975
SfuI	TTCGAA	1 cut(s)	287
SinI	GGWCC	2 cut(s)	1158, 2165
SlaI	CTCGAG	2 cut(s)	634, 1975
SmiMI	CAYNNNNRTG	1 cut(s)	398
SmlI	CTYRAG	4 cut(s)	634, 713, 1724, 1975
SmoI	CTYRAG	4 cut(s)	634, 713, 1724, 1975
SphI	GCATGC	1 cut(s)	1627
SspI	AATATT	2 cut(s)	913, 1927
SspMI	CTAG	8 cut(s)	1038, 1347, 1851, 2106, 2729, 2781, 3014, 3228
SstI	GAGCTC	4 cut(s)	225, 408, 2035, 2846
StyD4I	CCNGG	5 cut(s)	23, 246, 895, 1154, 2226
StyI	CCWWGG	4 cut(s)	1137, 1990, 2442, 2476
TaaI	ACNGT	8 cut(s)	19, 315, 1647, 1679, 1768, 2341, 2917, 3163
TaiI	ACGT	4 cut(s)	110, 972, 1638, 2777
TatI	WGTACW	2 cut(s)	3103, 3235
TauI	GCSGC	3 cut(s)	305, 753, 2611
Tru1I	TTAA	2 cut(s)	396, 614
Tru9I	TTAA	2 cut(s)	396, 614
TscAI	CASTG	4 cut(s)	117, 1332, 2826, 2922
TseFI	GTSAC	9 cut(s)	108, 315, 593, 965, 982, 1743, 1810, 2132, 2422
TseI	GCWGC	9 cut(s)	47, 337, 373, 1102, 1130, 1802, 2649, 2734, 3050
Tsp45I	GTSAC	9 cut(s)	108, 315, 593, 965, 982, 1743, 1810, 2132, 2422
TspDTI	ATGAA	5 cut(s)	805, 1608, 1731, 2736, 2754
TspGWI	ACGGA	3 cut(s)	598, 1798, 2235
TspRI	CASTG	4 cut(s)	117, 1332, 2826, 2922
Tth111I	GACNNNGTC	2 cut(s)	248, 440
Van91I	CCANNNNNTGG	1 cut(s)	1190
VneI	GTGCAC	1 cut(s)	2906
VpaK11BI	GGWCC	2 cut(s)	1158, 2165
XagI	CCTNNNNNAGG	2 cut(s)	2207, 2900
XapI	RAATTY	3 cut(s)	2117, 2254, 2462
XbaI	TCTAGA	1 cut(s)	2105
XceI	RCATGY	2 cut(s)	1296, 1627
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1765
XhoI	CTCGAG	2 cut(s)	634, 1975
XmiI	GTMKAC	1 cut(s)	696
XmnI	GAANNNNTTC	1 cut(s)	2981
XspI	CTAG	8 cut(s)	1038, 1347, 1851, 2106, 2729, 2781, 3014, 3228
ZraI	GACGTC	2 cut(s)	108, 2775
ZrmI	AGTACT	1 cut(s)	3105
Zsp2I	ATGCAT	1 cut(s)	1625

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.