Rorug01G0355900

AAA domain (Cdc48 subfamily)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007059
Physical Location & Seq
Forward (+)
47222533 .. 47223417
885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0355900.1

Sequence Viewer

Length: 729 bp
ATGGCCATGATAACCATTGCTTGCAGCTTAATCCTGCTAATCACCACCACCATAAACCACTCTTTTGCCGCTGCTCGAAGCTTAAGCAATTCAAACCCATCACACCCTCACAACCACCATCACAAACTCACATTCCTAATGAGAGATGTGCTCATGAATGTGACTCAACCCTCATCCAAGACCAAACCAGCAACCACCAAAGTGACTAGTCCTCAGCTGCCATTCTCAAAACCACTAGGACTATTTCCTCCAAATGGAGGAGTCCCCATCTCAGAAATCAATCCCATGCATACCACTACTTCTCCAGTTTCCGGAGTCTCCACTCAAACGCTAGATTTATCCAGCATTGGACTATTTTTTCCTGCTAGAGCCACACTGCAAGAACTGGAGTTTGGGGAAGTAACCGTGATCGATGAGGACATATTCGAAACTTTTACTTCAGGGTATGGTTCACTGGTCATTGGAAAAGCACAGGGGATATATGTTGCAAGCTCTGAAGATGGGAGTAGCCACATGATGGCCATGACTGCACATTTTGCTAATAGTCAATTTAAGGACGGATTGAGACTTTTCGGAGTTCATCGGAGGGATGTGCATGATGAATCTCATGTTTCTGTTATTGGTGGCATTGGAAAGTATGTGGGTGCAAATGGCTATGCAACAGTTAAGACAGTGAAAACTGAAGAAGCAGCCAGCAAGATGCTTAAGCTCAATGTCTATCTCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

242

Amino Acids

26.07

Weight (kDa)

7.22

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 126 - 241 5.2e-21 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 517
AccIII TCCGGA 1 cut(s) 311
AciI CCGC 1 cut(s) 69
AcoI YGGCCR 2 cut(s) 3, 519
AcuI CTGAAG 3 cut(s) 423, 516, 702
AfiI CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
AflII CTTAAG 2 cut(s) 82, 704
AgsI TTSAA 1 cut(s) 93
AhlI ACTAGT 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 5 cut(s) 27, 81, 217, 492, 709
AluI AGCT 5 cut(s) 27, 81, 217, 492, 709
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 2 cut(s) 322, 559
Aor13HI TCCGGA 1 cut(s) 311
AoxI GGCC 2 cut(s) 3, 519
ApeKI GCWGC 4 cut(s) 24, 71, 217, 689
AsuHPI GGTGA 1 cut(s) 34
AsuII TTCGAA 1 cut(s) 426
BalI TGGCCA 2 cut(s) 5, 521
Bbv12I GWGCWC 1 cut(s) 153
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 36, 58, 204, 701
BccI CCATC 5 cut(s) 106, 126, 275, 494, 511
BcoDI GTCTC 2 cut(s) 322, 559
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 5 cut(s) 207, 236, 332, 366, 727
BfrI CTTAAG 2 cut(s) 82, 704
BisI GCNGC 5 cut(s) 25, 69, 72, 218, 690
BlsI GCNGC 5 cut(s) 26, 70, 73, 219, 691
BmsI GCATC 1 cut(s) 690
BplI GAGNNNNNCTC 2 cut(s) 135, 167
BpmI CTGGAG 2 cut(s) 288, 407
Bpu10I CCTNAGC 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 426
Bsa29I ATCGAT 1 cut(s) 411
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
Bse1I ACTGG 3 cut(s) 305, 390, 459
Bse3DI GCAATG 1 cut(s) 15
BseAI TCCGGA 1 cut(s) 311
BseCI ATCGAT 1 cut(s) 411
BseGI GGATG 2 cut(s) 173, 595
BseLI CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 2 cut(s) 227, 285
BseNI ACTGG 3 cut(s) 305, 390, 459
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 4 cut(s) 36, 58, 204, 701
BsgI GTGCAG 1 cut(s) 513
BshFI GGCC 2 cut(s) 5, 521
BshVI ATCGAT 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
BsmAI GTCTC 2 cut(s) 322, 559
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 2 cut(s) 5, 521
Bsp119I TTCGAA 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 153
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 1 cut(s) 408
BspACI CCGC 1 cut(s) 69
BspANI GGCC 2 cut(s) 5, 521
BspCNI CTCAG 2 cut(s) 226, 284
BspDI ATCGAT 1 cut(s) 411
BspEI TCCGGA 1 cut(s) 311
BspHI TCATGA 1 cut(s) 153
BspT104I TTCGAA 1 cut(s) 426
BspTI CTTAAG 2 cut(s) 82, 704
BsrDI GCAATG 1 cut(s) 15
BsrI ACTGG 3 cut(s) 305, 390, 459
BssMI GATC 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 406, 664, 673
BstAFI CTTAAG 2 cut(s) 82, 704
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 426
BstC8I GCNNGC 3 cut(s) 22, 490, 694
BstDEI CTNAG 2 cut(s) 213, 271
BstF5I GGATG 2 cut(s) 173, 595
BstKTI GATC 1 cut(s) 411
BstMAI GTCTC 2 cut(s) 322, 559
BstMBI GATC 1 cut(s) 408
BstMWI GCNNNNNNNGC 2 cut(s) 527, 536
BstV1I GCAGC 4 cut(s) 36, 58, 204, 701
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 2 cut(s) 5, 521
BsuTUI ATCGAT 1 cut(s) 411
BtsCI GGATG 2 cut(s) 173, 595
BtsI GCAGTG 1 cut(s) 374
BtsIMutI CAGTG 3 cut(s) 374, 452, 678
Cac8I GCNNGC 3 cut(s) 22, 490, 694
CciI TCATGA 1 cut(s) 153
ClaI ATCGAT 1 cut(s) 411
CviAII CATG 7 cut(s) 7, 154, 286, 514, 523, 596, 608
DdeI CTNAG 2 cut(s) 213, 271
DpnI GATC 1 cut(s) 410
DpnII GATC 1 cut(s) 408
EaeI YGGCCR 2 cut(s) 3, 519
Eco57I CTGAAG 3 cut(s) 423, 516, 702
EcoT22I ATGCAT 1 cut(s) 291
FaeI CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
FaqI GGGAC 1 cut(s) 248
FatI CATG 7 cut(s) 6, 153, 285, 513, 522, 595, 607
Fnu4HI GCNGC 5 cut(s) 25, 69, 72, 218, 690
FokI GGATG 2 cut(s) 160, 602
Fsp4HI GCNGC 5 cut(s) 25, 69, 72, 218, 690
FspBI CTAG 5 cut(s) 207, 236, 332, 366, 727
GluI GCNGC 5 cut(s) 25, 69, 72, 218, 690
GsuI CTGGAG 2 cut(s) 288, 407
HaeIII GGCC 2 cut(s) 5, 521
HapII CCGG 1 cut(s) 312
Hin1II CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 4 cut(s) 163, 261, 315, 602
HpaII CCGG 1 cut(s) 312
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 452
Hpy188I TCNGA 4 cut(s) 274, 496, 575, 585
Hpy188III TCNNGA 2 cut(s) 154, 312
Hpy8I GTNNAC 1 cut(s) 452
HpyCH4III ACNGT 3 cut(s) 406, 664, 673
HpyCH4V TGCA 8 cut(s) 24, 289, 379, 488, 530, 595, 647, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 536
HpyF3I CTNAG 2 cut(s) 213, 271
Hsp92II CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
Kpn2I TCCGGA 1 cut(s) 311
Kzo9I GATC 1 cut(s) 408
Lsp1109I GCAGC 4 cut(s) 36, 58, 204, 701
LweI GCATC 1 cut(s) 690
MaeI CTAG 5 cut(s) 207, 236, 332, 366, 727
MaeIII GTNAC 3 cut(s) 160, 202, 400
MalI GATC 1 cut(s) 410
MboI GATC 1 cut(s) 408
MboII GAAGA 2 cut(s) 509, 695
MhlI GDGCHC 1 cut(s) 153
MlsI TGGCCA 2 cut(s) 5, 521
MluCI AATT 2 cut(s) 88, 548
MluNI TGGCCA 2 cut(s) 5, 521
MlyI GAGTC 3 cut(s) 157, 270, 324
MnlI CCTC 7 cut(s) 117, 181, 222, 251, 258, 409, 579
Mox20I TGGCCA 2 cut(s) 5, 521
Mph1103I ATGCAT 1 cut(s) 291
MroI TCCGGA 1 cut(s) 311
MscI TGGCCA 2 cut(s) 5, 521
MseI TTAA 5 cut(s) 29, 83, 552, 666, 705
MslI CAYNNNNRTG 2 cut(s) 158, 200
Msp20I TGGCCA 2 cut(s) 5, 521
MspA1I CMGCKG 2 cut(s) 71, 217
MspCI CTTAAG 2 cut(s) 82, 704
MspI CCGG 1 cut(s) 312
MwoI GCNNNNNNNGC 2 cut(s) 527, 536
NdeII GATC 1 cut(s) 408
NlaIII CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
NmuCI GTSAC 2 cut(s) 160, 202
NsiI ATGCAT 1 cut(s) 291
NspV TTCGAA 1 cut(s) 426
OliI CACNNNNGTG 1 cut(s) 200
PagI TCATGA 1 cut(s) 153
PfeI GAWTC 1 cut(s) 602
PflMI CCANNNNNTGG 1 cut(s) 517
PkrI GCNGC 5 cut(s) 26, 70, 73, 219, 691
PleI GAGTC 3 cut(s) 157, 269, 323
PpsI GAGTC 3 cut(s) 157, 269, 323
PvuII CAGCTG 1 cut(s) 217
RseI CAYNNNNRTG 2 cut(s) 158, 200
SaqAI TTAA 5 cut(s) 29, 83, 552, 666, 705
SatI GCNGC 5 cut(s) 25, 69, 72, 218, 690
Sau3AI GATC 1 cut(s) 408
SchI GAGTC 3 cut(s) 157, 270, 324
SduI GDGCHC 1 cut(s) 153
SetI ASST 5 cut(s) 29, 83, 219, 494, 711
SfaNI GCATC 1 cut(s) 690
SfuI TTCGAA 1 cut(s) 426
SmiMI CAYNNNNRTG 2 cut(s) 158, 200
SmlI CTYRAG 2 cut(s) 82, 704
SmoI CTYRAG 2 cut(s) 82, 704
SpeI ACTAGT 1 cut(s) 206
Sse9I AATT 2 cut(s) 88, 548
SsiI CCGC 1 cut(s) 69
SspMI CTAG 5 cut(s) 207, 236, 332, 366, 727
TaaI ACNGT 3 cut(s) 406, 664, 673
TaqI TCGA 3 cut(s) 76, 411, 426
TasI AATT 2 cut(s) 88, 548
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 602
Tru1I TTAA 5 cut(s) 29, 83, 552, 666, 705
Tru9I TTAA 5 cut(s) 29, 83, 552, 666, 705
TscAI CASTG 3 cut(s) 381, 459, 678
TseFI GTSAC 2 cut(s) 160, 202
TseI GCWGC 4 cut(s) 24, 71, 217, 689
Tsp45I GTSAC 2 cut(s) 160, 202
TspDTI ATGAA 3 cut(s) 170, 569, 615
TspGWI ACGGA 1 cut(s) 573
TspRI CASTG 3 cut(s) 381, 459, 678
Van91I CCANNNNNTGG 1 cut(s) 517
Vha464I CTTAAG 2 cut(s) 82, 704
XspI CTAG 5 cut(s) 207, 236, 332, 366, 727
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.