Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007059

Physical Location & Seq

Genomic Coordinates Forward (+)

29949408 .. 29954241

Gene Structure 4834 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1222400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3243 bp

ATGCCTACGCCGGTTACTGTAGCCAGGCAATGCCTAACGCCGGAGGCAGCAAACGCTTTGGACGAGGCCGTGGCGGTTGCGCGCCGAAGAGGCCACGGCCAAACGACGTCGCTGCACGCCATATCCGCTCTACTCTCCATCCCGTCCTCCGCGCTACGCGACGCCTGCGCGCGCGCCCGAAACTCCGCCTACTCGCCCCGACTGCAATTCAAGGCTCTGGAGCTCTGCCTCAGCGTCTCGCTCGACAGGGTCTCCTCGACCCAACTCGCCGACGACCCGCCCGTTTCGAACTCGCTCATGGCCGCCATCAAACGGTCCCAAGCCAACCAGCGGAGGCAGCCCGAAAATTACCACCTATACCACCAGCTCTCGCAGCAGTCCTCGATCTCCACCATTAAAGTGGAGCTCCAGCACCTGATTCTGTCGATTCTCGACGACCCTGTCGTCAGTCGGGTCTTCGCTGAAGCGGGGTTTCGGAGCTCGGAAATAAAGTTTGCAATTTTACGCCCTTTTCCTCAGATTCTCAGATACCCGCGCTCCAGAGGCCACCATCCTCTGTTTCTCTGCAACTTAGCCGAGTACGCGGACCCCGGTCGCCCGACCCGAACCGTTTTAACTGACGGGGACGAGAATTCGAGGAGAATAGGAGAGGTTCTCGGGAGAAACAGAGGGCGGAATCCTCTGCTTGTGGGCATATTCGCTCACGACGCACTCAAGAGCTTCGTGGAGGCTCTGGAGAAAAGAGACGGCGGCGTTTTGCCGGCGGACCTGTCTGGGTTGTCTGTAATTTCAGCGGAAAACGATGTCTCAAAGTTTATCACCGCAGATAGCGATGAAGGGTCGGTGAATTTGAGGTTCGGAGAGGTGGGTCGGGTCGCAGAGCAGAGTTTGGGGCCTGGGATTGTTCTGAACATTGGGGACCTGAAAGCGTTTGTGGCTGAGAACGCGGTGGCTGACTCAGTGAGTCACGTGGTAACCGAATTGACAAGGCTGCTGGAGCTTCAGCGCGGGAAAATTTGGTTGATTGGTGCAACGGCTAGCTATGGAAGTTACTTAAAGTTCGTGGAGAGGTTTCCATCTGTGGAAAAAGACTGGGACTTGCAGCTTCTGCCCATCACTTCTCTCAGAGGTGCTTCCATGGCTGAATCATATCCCAGGTCCAGGTTGATGGAGTCGTTTGTTCCATTTGGTGGATTCTTTTCAGCACCTTCTGACCTAAAGCTCCCAATAAGTAGCTCGTATCAATGTTTTCCCCGCAATCATCAGTGCAATGAAAAAAGTGAGCAAGAAGCGTATTCTGTTCCGATGGGAGGTATTATGGCTTCAGTGGCAGGTCAGCCCCCAGCTAGTTTGCCTTCTTGGTTGCAGATGGCTCCTCTTGGCACAAACAAGGGATTGGATATGAAGACCAAAGACGATGGTGTGTTATTGAGTGCCAAAGTTTCAGGATTGCAAAAGAAATGGGATGATAAATGCCAACATCTTCACGACTCTCGTCCCCTGCCGGAAGCAAACTTCTTTCCAACTATAGTAGGATTTCAGTCTCCTGAAGACAAGAGGTGCAATCACGACAACACCATCAATATATCTTCACGTAAAATTGAGTGCAAGAATGCAGATTCATGCATGGCCGCAGATGTGCAAACACAATCAAGCCTTCCTCCAAAGGCTAAGAATGACAGTTTCTCTTCCGAAGTCTGGGAAAAAACTTCAAAAGATGAAGATCTTGAGTCGGCTGGCCTCAGGTCACCCTGCATGTCCAACTCTAGTGTGGTTGATGGAACATCTGCCACATCAATCACTTCCGTCACCACAGATTTAGGGTTAGGAATATGCTCTTCTCCTGCTAGTAATACACCGAATAAACCTCCAGATCTGAATCAAGCACTTCAACAGGACATCTCGGGTTGCTTTTCCTCTAATATTGATTTAGTCAACGGGAATCTCTATTATACTCGATCATCATCTTGCTCGAATCCTGACAACCATGGGCAGTTTGATCCAAGTGATGTCAAGATGCTTTTTAGAGCTCTCTTTGAAAGGGTTGGCTGGCAAACTGATGCTGTAAGTGTAATTAGCCAAAGGATAGCTAACTGCCGATCTAGAAGTGAAAAATTCTGTGGAGCAAGTAACAGAAGGGATGCATGGTTCAATTTTACAGGACCTGATAGGTATGGTAAAAAGAAAATTGCCATTGCCCTTGCTGAGGTATTATACGGAAACCAGGAACAATTGATCTGTGCGGATCTAAATTCTCAGGATAGAATGATCCCTTCAGACACAAATCTTGATTGTTCAGTGGTGAATGGTTATGACATCAGGTTTAGAGGTAAGACAGTTCTTGATTATGTTGCCGGAGAATTGTGCAAGAAACCCTTGTCCATTGTCTTCTTAGAAAACGTAGACAAGGCTGATGTGGTGACTCAAAATGGTTTGTCCCAGGCCTTATTGACTGGTAAATTTTCCGATTCCCATGGAAGACAAGTAAGCACCAATAATGCGATATTTGTCACAACTTCGACATTCTCAAAGGGATGCAATATTCTTACTTCCACAAAGGGACCCTCCCACTATTCCGAGGAAAGAATCTTACAAGCGATAGAGCAGCCTGTGCAGATTACTATTGAATGTGCCTCTGAAGACAGCAGCAAAAGCAAAAACTGGAAGGCATCCACGAATCAACATTTTCTAAATAAAAGGAAACTGGTCGGTGTCAATGAACCTCTAGAGCAGCATGAAGTCTCAGAGATGCCCAAACGGGCGAACAAGACGTCAACTAGGTATCTGGATCTCAACCTTCCATCTGAAGAAAATGCAGTGGAAAACAGAGACGATGGGAGCTCTGAAAACGACTGGCCATCTGAAAACTCCAAGTCCTGGTTGCACGACTTCCTTGATCAGGTGGGCGAAACAGTGGTATTCAAGCCCGTTGATTTTGACGCACTTGCTGAGAAAATATCAAAGGAAATCAAGAATAGCTTCCGCAAGGTTGTCGACTCAGAGTGCCTGCTAGAAATCGATCCAGAAGTCGTGGAACTACTACTTGCAGCTCTATATTTGTCAAACAGGTCTCGGGTGGTGGAAGATTGGGTGGAGCAAGTACTCAGCAGGGCATTTGCAGAAGTTAAGAAGCGACATAACTCGAACACAGTCACTACCGTAAAACTTAAAACTTGCGAGGGGATTTGGTTGGAACGTCGAGCACCACAAACTTATCTTCTCCCTAGCATTGTACTAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_2 Clp_N

Protein Analysis

1081

Amino Acids

119.11

Weight (kDa)

6.2

Isoelectric Point (pI)

50.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NBD_SMAX1	PF23569	211 - 375	1.4e-19	SMAX1 nucleotide binding domain
AAA_2	PF07724	716 - 845	3.2e-12	AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1	PF26587	978 - 1061	7.8e-30	SMAX1-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007059)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40130 AT2G40130
fragaria_vesca	FvH4_7g24540 FvH4_7g24540
malus_domestica	MD01G1153400.v1.1 MD07G1222400.v1.1
prunus_persica	Prupe.2G250700_v2.0.a1 Prupe.2G250700_v2.0.a1
pyrus_communis	pycom01g17100 pycom07g20100 pycom07g20110
rosa_chinensis	RchiOBHm_Chr1g0370371
rosa_laevigata	RLG00000027054
rosa_multiflora	Rmu_co8224134.1_g000001 Rmu_co8315027.1_g000001 Rmu_sc0006707.1_g000030 Rmu_sc0029129.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286640
rosa_rugosa	Rorug01G0355800 Rorug01G0355900
rosa_samantha	Rh1AG364200 Rh1BG327500 Rh1CG340500 Rh1DG357800
rosa_wichuraiana	Rw1G032050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	440, 443
AatII	GACGTC	2 cut(s)	110, 2774
Acc36I	ACCTGC	1 cut(s)	1322
AccB7I	CCANNNNNTGG	2 cut(s)	1190, 2877
AccBSI	CCGCTC	1 cut(s)	128
AccI	GTMKAC	2 cut(s)	2403, 2994
AclWI	GGATC	5 cut(s)	1994, 2253, 2263, 2796, 3014
AcoI	YGGCCR	4 cut(s)	97, 300, 1629, 2855
AcsI	RAATTY	6 cut(s)	631, 847, 1014, 2114, 2251, 2459
AcuI	CTGAAG	7 cut(s)	483, 986, 1308, 1569, 2259, 2658, 2826
AcvI	CACGTG	1 cut(s)	970
AcyI	GRCGYC	3 cut(s)	107, 162, 2771
AfaI	GTAC	3 cut(s)	581, 3102, 3234
AgsI	TTSAA	7 cut(s)	211, 1713, 1892, 2039, 2152, 2627, 2923
AjnI	CCWGG	7 cut(s)	23, 895, 1154, 1160, 2223, 2439, 2876
AloI	GAACNNNNNNTCC	2 cut(s)	2132, 2164
Alw21I	GWGCWC	6 cut(s)	225, 408, 482, 2032, 2843, 3205
Alw26I	GTCTC	8 cut(s)	241, 256, 738, 811, 1548, 2746, 2823, 3075
AlwI	GGATC	5 cut(s)	1994, 2253, 2263, 2796, 3014
AlwNI	CAGNNNCTG	3 cut(s)	415, 1108, 2165
Ama87I	CYCGRG	3 cut(s)	656, 1903, 3072
ApoI	RAATTY	6 cut(s)	631, 847, 1014, 2114, 2251, 2459
ArsI	GACNNNNNNTTYG	2 cut(s)	2756, 2788
Asp700I	GAANNNNTTC	1 cut(s)	2978
AspLEI	GCGC	9 cut(s)	82, 84, 154, 170, 172, 174, 176, 537, 1008
AspS9I	GGNCC	8 cut(s)	315, 586, 766, 893, 919, 1158, 2162, 2561
AsuC2I	CCSGG	1 cut(s)	591
AsuHPI	GGTGA	6 cut(s)	811, 856, 1740, 1801, 2314, 2431
AsuII	TTCGAA	1 cut(s)	287
AsuNHI	GCTAGC	1 cut(s)	1037
AvaI	CYCGRG	3 cut(s)	656, 1903, 3072
AvaII	GGWCC	7 cut(s)	315, 586, 766, 919, 1158, 2162, 2561
AxyI	CCTNAGG	1 cut(s)	1742
BalI	TGGCCA	1 cut(s)	2857
BanII	GRGCYC	5 cut(s)	225, 408, 482, 2032, 2843
BarI	GAAGNNNNNNTAC	2 cut(s)	1306, 1338
BbrPI	CACGTG	1 cut(s)	970
BbsI	GAAGAC	6 cut(s)	448, 1412, 1557, 2380, 2485, 2646
Bbv12I	GWGCWC	6 cut(s)	225, 408, 482, 2032, 2843, 3205
BbvCI	CCTCAGC	2 cut(s)	230, 2205
BceAI	ACGGC	4 cut(s)	53, 112, 763, 1050
BcgI	CGANNNNNNTGC	4 cut(s)	94, 128, 2974, 3008
BciT130I	CCWGG	7 cut(s)	25, 897, 1156, 1162, 2225, 2441, 2878
BclI	TGATCA	1 cut(s)	2896
BcnI	CCSGG	1 cut(s)	591
BcoDI	GTCTC	8 cut(s)	241, 256, 738, 811, 1548, 2746, 2823, 3075
BfaI	CTAG	9 cut(s)	1038, 1347, 1767, 1848, 2103, 2726, 2778, 3011, 3225
BfmI	CTRYAG	2 cut(s)	18, 1527
BfuAI	ACCTGC	1 cut(s)	1322
BglI	GCCNNNNNGGC	1 cut(s)	90
BglII	AGATCT	2 cut(s)	1723, 1873
BmcAI	AGTACT	1 cut(s)	3102
Bme1390I	CCNGG	8 cut(s)	25, 591, 897, 1156, 1162, 2225, 2441, 2878
Bme18I	GGWCC	7 cut(s)	315, 586, 766, 919, 1158, 2162, 2561
BmeT110I	CYCGRG	3 cut(s)	656, 1903, 3072
BmgT120I	GGNCC	8 cut(s)	315, 586, 766, 893, 919, 1158, 2162, 2561
BmiI	GGNNCC	7 cut(s)	317, 588, 894, 920, 1374, 2562, 2563
BmrFI	CCNGG	8 cut(s)	25, 591, 897, 1156, 1162, 2225, 2441, 2878
BmrI	ACTGGG	1 cut(s)	1102
BmsI	GCATC	6 cut(s)	2007, 2050, 2131, 2525, 2678, 2739
BmtI	GCTAGC	1 cut(s)	1041
BmuI	ACTGGG	1 cut(s)	1102
BoxI	GACNNNNGTC	2 cut(s)	591, 1494
BpiI	GAAGAC	6 cut(s)	448, 1412, 1557, 2380, 2485, 2646
BplI	GAGNNNNNCTC	2 cut(s)	639, 671
BpmI	CTGGAG	6 cut(s)	239, 392, 523, 755, 1016, 1854
Bpu10I	CCTNAGC	2 cut(s)	230, 2205
Bpu14I	TTCGAA	1 cut(s)	287
BpuEI	CTTGAG	2 cut(s)	698, 1748
BpuMI	CCSGG	1 cut(s)	591
Bsa29I	ATCGAT	1 cut(s)	3018
BsaAI	YACGTR	2 cut(s)	970, 1595
BsaBI	GATNNNNATC	3 cut(s)	1722, 1878, 1963
BsaHI	GRCGYC	3 cut(s)	107, 162, 2771
BsaI	GGTCTC	2 cut(s)	256, 3075
BsaXI	ACNNNNNCTCC	4 cut(s)	236, 266, 521, 551
Bse118I	RCCGGY	2 cut(s)	10, 760
Bse1I	ACTGG	5 cut(s)	1097, 2458, 2666, 2709, 2858
Bse21I	CCTNAGG	1 cut(s)	1742
Bse3DI	GCAATG	3 cut(s)	35, 1276, 2193
Bse8I	GATNNNNATC	3 cut(s)	1722, 1878, 1963
BseBI	CCWGG	7 cut(s)	25, 897, 1156, 1162, 2225, 2441, 2878
BseCI	ATCGAT	1 cut(s)	3018
BseGI	GGATG	6 cut(s)	138, 550, 1471, 2146, 2540, 2669
BseJI	GATNNNNATC	3 cut(s)	1722, 1878, 1963
BseMI	GCAATG	3 cut(s)	35, 1276, 2193
BseNI	ACTGG	5 cut(s)	1097, 2458, 2666, 2709, 2858
BsePI	GCGCGC	4 cut(s)	80, 168, 170, 172
BseRI	GAGGAG	3 cut(s)	244, 652, 1365
BseYI	CCCAGC	1 cut(s)	1342
BsgI	GTGCAG	2 cut(s)	98, 2633
Bsh1285I	CGRYCG	1 cut(s)	595
BshVI	ATCGAT	1 cut(s)	3018
BsiEI	CGRYCG	1 cut(s)	595
BsiHKAI	GWGCWC	6 cut(s)	225, 408, 482, 2032, 2843, 3205
BsiHKCI	CYCGRG	3 cut(s)	656, 1903, 3072
BsiSI	CCGG	6 cut(s)	11, 41, 591, 761, 1505, 2355
BslFI	GGGAC	7 cut(s)	301, 638, 932, 1109, 1482, 2422, 2574
BsmAI	GTCTC	8 cut(s)	241, 256, 738, 811, 1548, 2746, 2823, 3075
BsmBI	CGTCTC	3 cut(s)	241, 738, 2823
BsmFI	GGGAC	7 cut(s)	301, 638, 932, 1109, 1482, 2422, 2574
BsmI	GAATGC	1 cut(s)	1618
Bso31I	GGTCTC	2 cut(s)	256, 3075
BsoBI	CYCGRG	3 cut(s)	656, 1903, 3072
Bsp119I	TTCGAA	1 cut(s)	287
Bsp1286I	GDGCHC	6 cut(s)	225, 408, 482, 2032, 2843, 3205
Bsp19I	CCATGG	3 cut(s)	1137, 1987, 2473
BspDI	ATCGAT	1 cut(s)	3018
BspLI	GGNNCC	7 cut(s)	317, 588, 894, 920, 1374, 2562, 2563
BspMI	ACCTGC	1 cut(s)	1322
BspOI	GCTAGC	1 cut(s)	1041
BspPI	GGATC	5 cut(s)	1994, 2253, 2263, 2796, 3014
BspQI	GCTCTTC	1 cut(s)	1843
BspT104I	TTCGAA	1 cut(s)	287
BspTNI	GGTCTC	2 cut(s)	256, 3075
BsrBI	CCGCTC	1 cut(s)	128
BsrDI	GCAATG	3 cut(s)	35, 1276, 2193
BsrFI	RCCGGY	2 cut(s)	10, 760
BsrI	ACTGG	5 cut(s)	1097, 2458, 2666, 2709, 2858
BssAI	RCCGGY	2 cut(s)	10, 760
BssHII	GCGCGC	4 cut(s)	80, 168, 170, 172
BssNI	GRCGYC	3 cut(s)	107, 162, 2771
BssT1I	CCWWGG	3 cut(s)	1137, 1987, 2473
Bst2UI	CCWGG	7 cut(s)	25, 897, 1156, 1162, 2225, 2441, 2878
Bst4CI	ACNGT	8 cut(s)	19, 315, 610, 1682, 2338, 2914, 3151, 3160
Bst6I	CTCTTC	3 cut(s)	82, 1693, 1843
BstACI	GRCGYC	3 cut(s)	107, 162, 2771
BstAPI	GCANNNNNTGC	2 cut(s)	1108, 2611
BstBAI	YACGTR	2 cut(s)	970, 1595
BstBI	TTCGAA	1 cut(s)	287
BstDSI	CCRYGG	5 cut(s)	69, 94, 1137, 1987, 2473
BstEII	GGTNACC	2 cut(s)	973, 1746
BstENI	CCTNNNNNAGG	2 cut(s)	2204, 2897
BstF5I	GGATG	6 cut(s)	138, 550, 1471, 2146, 2540, 2669
BstHHI	GCGC	9 cut(s)	82, 84, 154, 170, 172, 174, 176, 537, 1008
BstMAI	GTCTC	8 cut(s)	241, 256, 738, 811, 1548, 2746, 2823, 3075
BstMCI	CGRYCG	1 cut(s)	595
BstNI	CCWGG	7 cut(s)	25, 897, 1156, 1162, 2225, 2441, 2878
BstNSI	RCATGY	1 cut(s)	1759
BstPAI	GACNNNNGTC	2 cut(s)	591, 1494
BstPI	GGTNACC	2 cut(s)	973, 1746
BstSCI	CCNGG	8 cut(s)	23, 589, 895, 1154, 1160, 2223, 2439, 2876
BstSFI	CTRYAG	2 cut(s)	18, 1527
BstV2I	GAAGAC	6 cut(s)	448, 1412, 1557, 2380, 2485, 2646
BstX2I	RGATCY	4 cut(s)	1723, 1873, 2245, 2788
BstXI	CCANNNNNNTGG	2 cut(s)	400, 1168
BstYI	RGATCY	4 cut(s)	1723, 1873, 2245, 2788
Bsu15I	ATCGAT	1 cut(s)	3018
Bsu36I	CCTNAGG	1 cut(s)	1742
BsuTUI	ATCGAT	1 cut(s)	3018
BtgI	CCRYGG	5 cut(s)	69, 94, 1137, 1987, 2473
BtgZI	GCGATG	1 cut(s)	846
BtsCI	GGATG	6 cut(s)	138, 550, 1471, 2146, 2540, 2669
BtsI	GCAGTG	1 cut(s)	2823
BtsIMutI	CAGTG	6 cut(s)	966, 1271, 1332, 2304, 2823, 2919
BveI	ACCTGC	1 cut(s)	1322
CaiI	CAGNNNCTG	3 cut(s)	415, 1108, 2165
CfoI	GCGC	9 cut(s)	82, 84, 154, 170, 172, 174, 176, 537, 1008
Cfr10I	RCCGGY	2 cut(s)	10, 760
Cfr13I	GGNCC	8 cut(s)	315, 586, 766, 893, 919, 1158, 2162, 2561
ClaI	ATCGAT	1 cut(s)	3018
CseI	GACGC	4 cut(s)	170, 223, 716, 2948
Csp6I	GTAC	3 cut(s)	580, 3101, 3233
CviAII	CATG	9 cut(s)	298, 1138, 1623, 1627, 1756, 1988, 2145, 2474, 2735
CviQI	GTAC	3 cut(s)	580, 3101, 3233
DrdI	GACNNNNNNGTC	2 cut(s)	440, 443
DseDI	GACNNNNNNGTC	2 cut(s)	440, 443
EaeI	YGGCCR	4 cut(s)	97, 300, 1629, 2855
Eam1104I	CTCTTC	3 cut(s)	82, 1693, 1843
EarI	CTCTTC	3 cut(s)	82, 1693, 1843
EciI	GGCGGA	3 cut(s)	175, 688, 779
Ecl136II	GAGCTC	5 cut(s)	223, 406, 480, 2030, 2841
Eco130I	CCWWGG	3 cut(s)	1137, 1987, 2473
Eco147I	AGGCCT	1 cut(s)	2444
Eco24I	GRGCYC	5 cut(s)	225, 408, 482, 2032, 2843
Eco31I	GGTCTC	2 cut(s)	256, 3075
Eco47I	GGWCC	7 cut(s)	315, 586, 766, 919, 1158, 2162, 2561
Eco53kI	GAGCTC	5 cut(s)	223, 406, 480, 2030, 2841
Eco57I	CTGAAG	7 cut(s)	483, 986, 1308, 1569, 2259, 2658, 2826
Eco72I	CACGTG	1 cut(s)	970
Eco81I	CCTNAGG	1 cut(s)	1742
Eco88I	CYCGRG	3 cut(s)	656, 1903, 3072
Eco91I	GGTNACC	2 cut(s)	973, 1746
EcoICRI	GAGCTC	5 cut(s)	223, 406, 480, 2030, 2841
EcoNI	CCTNNNNNAGG	2 cut(s)	2204, 2897
EcoO109I	RGGNCCY	4 cut(s)	893, 919, 2162, 2561
EcoO65I	GGTNACC	2 cut(s)	973, 1746
EcoRI	GAATTC	1 cut(s)	631
EcoRII	CCWGG	7 cut(s)	23, 895, 1154, 1160, 2223, 2439, 2876
EcoT14I	CCWWGG	3 cut(s)	1137, 1987, 2473
EcoT22I	ATGCAT	2 cut(s)	1628, 2146
EcoT38I	GRGCYC	5 cut(s)	225, 408, 482, 2032, 2843
ErhI	CCWWGG	3 cut(s)	1137, 1987, 2473
Esp3I	CGTCTC	3 cut(s)	241, 738, 2823
FaeI	CATG	9 cut(s)	301, 1141, 1626, 1630, 1759, 1991, 2148, 2477, 2738
FalI	AAGNNNNNCTT	4 cut(s)	2360, 2392, 2963, 2995
FaqI	GGGAC	7 cut(s)	301, 638, 932, 1109, 1482, 2422, 2574
FatI	CATG	9 cut(s)	297, 1137, 1622, 1626, 1755, 1987, 2144, 2473, 2734
FauI	CCCGC	5 cut(s)	285, 460, 540, 1001, 1262
FbaI	TGATCA	1 cut(s)	2896
FblI	GTMKAC	2 cut(s)	2403, 2994
FokI	GGATG	6 cut(s)	125, 537, 1478, 2153, 2547, 2656
FriOI	GRGCYC	5 cut(s)	225, 408, 482, 2032, 2843
FspBI	CTAG	9 cut(s)	1038, 1347, 1767, 1848, 2103, 2726, 2778, 3011, 3225
GlaI	GCGC	9 cut(s)	81, 83, 153, 169, 171, 173, 175, 536, 1007
GsaI	CCCAGC	1 cut(s)	1346
GsuI	CTGGAG	6 cut(s)	239, 392, 523, 755, 1016, 1854
HapII	CCGG	6 cut(s)	11, 41, 591, 761, 1505, 2355
HgaI	GACGC	4 cut(s)	170, 223, 716, 2948
HhaI	GCGC	9 cut(s)	82, 84, 154, 170, 172, 174, 176, 537, 1008
Hin1I	GRCGYC	3 cut(s)	107, 162, 2771
Hin1II	CATG	9 cut(s)	301, 1141, 1626, 1630, 1759, 1991, 2148, 2477, 2738
Hin6I	GCGC	9 cut(s)	80, 82, 152, 168, 170, 172, 174, 535, 1006
HinP1I	GCGC	9 cut(s)	80, 82, 152, 168, 170, 172, 174, 535, 1006
HincII	GTYRAC	3 cut(s)	1936, 2775, 2995
HindII	GTYRAC	3 cut(s)	1936, 2775, 2995
HpaII	CCGG	6 cut(s)	11, 41, 591, 761, 1505, 2355
HphI	GGTGA	6 cut(s)	811, 856, 1740, 1801, 2314, 2431
Hpy166II	GTNNAC	4 cut(s)	1936, 2404, 2775, 2995
Hpy8I	GTNNAC	4 cut(s)	1936, 2404, 2775, 2995
Hpy99I	CGWCG	7 cut(s)	109, 112, 164, 275, 437, 710, 3201
HpyAV	CCTTC	8 cut(s)	830, 1218, 1365, 1667, 2130, 2283, 2659, 2807
HpyCH4III	ACNGT	8 cut(s)	19, 315, 610, 1682, 2338, 2914, 3151, 3160
HpyCH4IV	ACGT	6 cut(s)	107, 969, 1594, 2400, 2771, 3196
HpySE526I	ACGT	6 cut(s)	107, 969, 1594, 2400, 2771, 3196
Hsp92I	GRCGYC	3 cut(s)	107, 162, 2771
Hsp92II	CATG	9 cut(s)	301, 1141, 1626, 1630, 1759, 1991, 2148, 2477, 2738
HspAI	GCGC	9 cut(s)	80, 82, 152, 168, 170, 172, 174, 535, 1006
KflI	GGGWCCC	1 cut(s)	2561
KroI	GCCGGC	1 cut(s)	760
KroNI	GCCGGC	1 cut(s)	762
Ksp22I	TGATCA	1 cut(s)	2896
LguI	GCTCTTC	1 cut(s)	1843
LweI	GCATC	6 cut(s)	2007, 2050, 2131, 2525, 2678, 2739
MaeI	CTAG	9 cut(s)	1038, 1347, 1767, 1848, 2103, 2726, 2778, 3011, 3225
MaeII	ACGT	6 cut(s)	107, 969, 1594, 2400, 2771, 3196
MauBI	CGCGCGCG	1 cut(s)	170
MbiI	CCGCTC	1 cut(s)	128
MfeI	CAATTG	1 cut(s)	2231
MflI	RGATCY	4 cut(s)	1723, 1873, 2245, 2788
MhlI	GDGCHC	6 cut(s)	225, 408, 482, 2032, 2843, 3205
MlsI	TGGCCA	1 cut(s)	2857
MluNI	TGGCCA	1 cut(s)	2857
MlyI	GAGTC	7 cut(s)	950, 973, 1181, 1484, 1739, 2416, 2990
MmeI	TCCRAC	3 cut(s)	1547, 1785, 3171
Mox20I	TGGCCA	1 cut(s)	2857
Mph1103I	ATGCAT	2 cut(s)	1628, 2146
MroNI	GCCGGC	1 cut(s)	760
MroXI	GAANNNNTTC	1 cut(s)	2978
MscI	TGGCCA	1 cut(s)	2857
MseI	TTAA	5 cut(s)	396, 614, 1055, 3126, 3168
MslI	CAYNNNNRTG	1 cut(s)	398
Msp20I	TGGCCA	1 cut(s)	2857
MspA1I	CMGCKG	2 cut(s)	331, 794
MspI	CCGG	6 cut(s)	11, 41, 591, 761, 1505, 2355
MspR9I	CCNGG	8 cut(s)	25, 591, 897, 1156, 1162, 2225, 2441, 2878
MunI	CAATTG	1 cut(s)	2231
Mva1269I	GAATGC	1 cut(s)	1618
MvaI	CCWGG	7 cut(s)	25, 897, 1156, 1162, 2225, 2441, 2878
NaeI	GCCGGC	1 cut(s)	762
NciI	CCSGG	1 cut(s)	591
NcoI	CCATGG	3 cut(s)	1137, 1987, 2473
NgoMIV	GCCGGC	1 cut(s)	760
NheI	GCTAGC	1 cut(s)	1037
NlaIII	CATG	9 cut(s)	301, 1141, 1626, 1630, 1759, 1991, 2148, 2477, 2738
NlaIV	GGNNCC	7 cut(s)	317, 588, 894, 920, 1374, 2562, 2563
NmeAIII	GCCGAG	1 cut(s)	601
NmuCI	GTSAC	6 cut(s)	965, 1746, 1807, 2419, 2509, 3151
NsiI	ATGCAT	2 cut(s)	1628, 2146
NspI	RCATGY	1 cut(s)	1759
NspV	TTCGAA	1 cut(s)	287
PauI	GCGCGC	4 cut(s)	80, 168, 170, 172
PceI	AGGCCT	1 cut(s)	2444
PciSI	GCTCTTC	1 cut(s)	1843
PcsI	WCGNNNNNNNCGW	5 cut(s)	60, 279, 441, 601, 975
PctI	GAATGC	1 cut(s)	1618
PdiI	GCCGGC	1 cut(s)	762
PdmI	GAANNNNTTC	1 cut(s)	2978
PflFI	GACNNNGTC	2 cut(s)	248, 440
PflMI	CCANNNNNTGG	2 cut(s)	1190, 2877
PleI	GAGTC	7 cut(s)	950, 972, 1180, 1484, 1738, 2416, 2990
PmaCI	CACGTG	1 cut(s)	970
PmlI	CACGTG	1 cut(s)	970
PpsI	GAGTC	7 cut(s)	950, 972, 1180, 1484, 1738, 2416, 2990
Ppu21I	YACGTR	2 cut(s)	970, 1595
PpuMI	RGGWCCY	3 cut(s)	919, 2162, 2561
PshAI	GACNNNNGTC	2 cut(s)	591, 1494
Psp124BI	GAGCTC	5 cut(s)	225, 408, 482, 2032, 2843
Psp5II	RGGWCCY	3 cut(s)	919, 2162, 2561
Psp6I	CCWGG	7 cut(s)	23, 895, 1154, 1160, 2223, 2439, 2876
PspCI	CACGTG	1 cut(s)	970
PspEI	GGTNACC	2 cut(s)	973, 1746
PspFI	CCCAGC	1 cut(s)	1342
PspGI	CCWGG	7 cut(s)	23, 895, 1154, 1160, 2223, 2439, 2876
PspN4I	GGNNCC	7 cut(s)	317, 588, 894, 920, 1374, 2562, 2563
PspPI	GGNCC	8 cut(s)	315, 586, 766, 893, 919, 1158, 2162, 2561
PspPPI	RGGWCCY	3 cut(s)	919, 2162, 2561
PstNI	CAGNNNCTG	3 cut(s)	415, 1108, 2165
PsuI	RGATCY	4 cut(s)	1723, 1873, 2245, 2788
PsyI	GACNNNGTC	2 cut(s)	248, 440
PteI	GCGCGC	4 cut(s)	80, 168, 170, 172
RsaI	GTAC	3 cut(s)	581, 3102, 3234
RsaNI	GTAC	3 cut(s)	580, 3101, 3233
RseI	CAYNNNNRTG	1 cut(s)	398
SacI	GAGCTC	5 cut(s)	225, 408, 482, 2032, 2843
SalI	GTCGAC	1 cut(s)	2993
SapI	GCTCTTC	1 cut(s)	1843
SaqAI	TTAA	5 cut(s)	396, 614, 1055, 3126, 3168
Sau96I	GGNCC	8 cut(s)	315, 586, 766, 893, 919, 1158, 2162, 2561
ScaI	AGTACT	1 cut(s)	3102
SchI	GAGTC	7 cut(s)	950, 973, 1181, 1484, 1739, 2416, 2990
ScrFI	CCNGG	8 cut(s)	25, 591, 897, 1156, 1162, 2225, 2441, 2878
SduI	GDGCHC	6 cut(s)	225, 408, 482, 2032, 2843, 3205
SfaNI	GCATC	6 cut(s)	2007, 2050, 2131, 2525, 2678, 2739
SfcI	CTRYAG	2 cut(s)	18, 1527
SfuI	TTCGAA	1 cut(s)	287
SinI	GGWCC	7 cut(s)	315, 586, 766, 919, 1158, 2162, 2561
SmiMI	CAYNNNNRTG	1 cut(s)	398
SmlI	CTYRAG	2 cut(s)	713, 1727
SmoI	CTYRAG	2 cut(s)	713, 1727
SseBI	AGGCCT	1 cut(s)	2444
SspI	AATATT	2 cut(s)	1924, 2542
SspMI	CTAG	9 cut(s)	1038, 1347, 1767, 1848, 2103, 2726, 2778, 3011, 3225
SstI	GAGCTC	5 cut(s)	225, 408, 482, 2032, 2843
StuI	AGGCCT	1 cut(s)	2444
StyD4I	CCNGG	8 cut(s)	23, 589, 895, 1154, 1160, 2223, 2439, 2876
StyI	CCWWGG	3 cut(s)	1137, 1987, 2473
TaaI	ACNGT	8 cut(s)	19, 315, 610, 1682, 2338, 2914, 3151, 3160
TaiI	ACGT	6 cut(s)	110, 972, 1597, 2403, 2774, 3199
TatI	WGTACW	2 cut(s)	3100, 3232
TauI	GCSGC	3 cut(s)	305, 753, 1634
Tru1I	TTAA	5 cut(s)	396, 614, 1055, 3126, 3168
Tru9I	TTAA	5 cut(s)	396, 614, 1055, 3126, 3168
TscAI	CASTG	6 cut(s)	966, 1271, 1332, 2304, 2823, 2919
TseFI	GTSAC	6 cut(s)	965, 1746, 1807, 2419, 2509, 3151
Tsp45I	GTSAC	6 cut(s)	965, 1746, 1807, 2419, 2509, 3151
TspDTI	ATGAA	7 cut(s)	849, 1287, 1418, 1611, 1734, 2733, 2751
TspGWI	ACGGA	2 cut(s)	1795, 2232
TspRI	CASTG	6 cut(s)	966, 1271, 1332, 2304, 2823, 2919
Tth111I	GACNNNGTC	2 cut(s)	248, 440
Van91I	CCANNNNNTGG	2 cut(s)	1190, 2877
VpaK11BI	GGWCC	7 cut(s)	315, 586, 766, 919, 1158, 2162, 2561
XagI	CCTNNNNNAGG	2 cut(s)	2204, 2897
XapI	RAATTY	6 cut(s)	631, 847, 1014, 2114, 2251, 2459
XbaI	TCTAGA	2 cut(s)	2102, 2725
XceI	RCATGY	1 cut(s)	1759
XcmI	CCANNNNNNNNNTGG	2 cut(s)	397, 1768
XmiI	GTMKAC	2 cut(s)	2403, 2994
XmnI	GAANNNNTTC	1 cut(s)	2978
XspI	CTAG	9 cut(s)	1038, 1347, 1767, 1848, 2103, 2726, 2778, 3011, 3225
ZraI	GACGTC	2 cut(s)	108, 2772
ZrmI	AGTACT	1 cut(s)	3102
Zsp2I	ATGCAT	2 cut(s)	1628, 2146

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.