pycom07g20110

AAA domain (Cdc48 subfamily)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007059
Physical Location & Seq
Forward (+)
22289049 .. 22290275
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20110.1

Sequence Viewer

Length: 1227 bp
ATGCTTTTTAGAGCTCTCTTTGAAAGGGTTGGCTGGCAAACTGATGCTGTAAGTGTAATTAGCCAAAGGATAGCTAACTGCCGATCTATAAGTGAAAAGTTCCGTGGAGCAAGTAACAAAAGGGATGTATGGTTCAATTTTACAGGACCTGATAGGTATGGTAAAAAGAAAATTGCCATTGCCCTTGCTGAGGTATTATACGGAAACCAGGAACAATTGATCTGTGCGGATCTAAATTCTCAGGATAGAATGATCCCTTCAGACACAAATCTTGATTGTCCACTGGTGAATGGTTATGACGTCAGGTTTAGAGGTAAGACGGTTATTGATTATGTTGCCGGAGAATTGTGCAAGAAACCCTTGTCCATTGTCTTCTTAGAAAATGTAGACAAGGCTGATGTGGTGACTCAAAATTGTTTGTCCCAGGCTTTATTGACTGGTAAATTTTCCGATTCCCATGGAAGACAAGTAAGCACCAATAATGCGATATTTGTTACAACTTCGACATTCTCAAAGGGATGCAATATTCTTACTTCCACGAAGAGACCCTCCCACTATTCCGAGGAAAGAATCTTACAAGCGAAAGAGCAGCCTGTGCAGATTACTATTGAATGTGCCTCTGAAGACAACAGCAAAAGCAAAAACTGGAAGGCATCCACGAATCAACATTTTCTAAATAAAAGGAAACTGGTTGGTGTCAATGAACCTCTAGAGCAGCATGAAGTCTCAGAGATGCCCAAACGGGCTAACAAGACGTCAACTAGGTATCTGGATCTCAACCTTCCATCTGAAGAAAATGCAGTGGAAAACACAGACGATGGGAGCTATGAAAACGACTGGCTATCTGAAAACTCCAAGTCCTGGTTGCATGGCTTCCTTGATCAGGTGGACGAAACAGTGGTATTCAAGCCCGTTGATTTTGACACACTTGCTGAGAAGATATCAAAGGAAATCAAGAATAGCTTCCACAAGTTTGTCGACTCAGAGTGCCTGCTAGAAATCGATCCAGAAGTCCTGGAACAACTACTTGCAGCTCTATATTTGTCAAACAGGTCTTGGGTGGTGGAAGATTGGGTGGAGCAAGTACTCAGCAGGGCATTTGCAGAAGTTAAGAAGCGGCATAACTCGAACAGAATCACTACCGTAAAACTTAAAACTTGCGAGGCGATTTGGTTGGAACGTCAAGCACCGCAAACTTATGTTCTCCCTAGCATTGTACTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

409

Amino Acids

46.45

Weight (kDa)

6.07

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 44 - 176 4.8e-13 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 306 - 389 2.2e-30 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 303, 758
AccB7I CCANNNNNTGG 1 cut(s) 861
AccI GTMKAC 2 cut(s) 387, 978
AciI CCGC 3 cut(s) 227, 1117, 1190
AclWI GGATC 4 cut(s) 237, 247, 780, 998
AcsI RAATTY 2 cut(s) 235, 443
AcuI CTGAAG 3 cut(s) 243, 642, 810
AcyI GRCGYC 2 cut(s) 300, 755
AfaI GTAC 2 cut(s) 1086, 1218
AfiI CCNNNNNNNGG 3 cut(s) 190, 861, 883
AgsI TTSAA 4 cut(s) 23, 136, 611, 907
AjnI CCWGG 4 cut(s) 207, 423, 860, 1014
AloI GAACNNNNNNTCC 2 cut(s) 116, 148
AluBI AGCT 5 cut(s) 14, 74, 825, 963, 1034
AluI AGCT 5 cut(s) 14, 74, 825, 963, 1034
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 2 cut(s) 538, 730
AlwI GGATC 4 cut(s) 237, 247, 780, 998
AlwNI CAGNNNCTG 1 cut(s) 149
ApeKI GCWGC 3 cut(s) 589, 715, 1031
ApoI RAATTY 2 cut(s) 235, 443
Asp700I GAANNNNTTC 1 cut(s) 962
AspS9I GGNCC 1 cut(s) 146
AsuHPI GGTGA 2 cut(s) 298, 415
AvaII GGWCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 3 cut(s) 364, 469, 630
Bbv12I GWGCWC 1 cut(s) 16
BbvCI CCTCAGC 1 cut(s) 189
BbvI GCAGC 3 cut(s) 601, 727, 1043
BccI CCATC 2 cut(s) 793, 812
BciT130I CCWGG 4 cut(s) 209, 425, 862, 1016
BclI TGATCA 1 cut(s) 880
BcoDI GTCTC 2 cut(s) 538, 730
BfaI CTAG 4 cut(s) 710, 762, 995, 1209
BisI GCNGC 4 cut(s) 590, 716, 1032, 1118
BlsI GCNGC 4 cut(s) 591, 717, 1033, 1119
BmcAI AGTACT 1 cut(s) 1086
Bme1390I CCNGG 4 cut(s) 209, 425, 862, 1016
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 146
BmrFI CCNGG 4 cut(s) 209, 425, 862, 1016
BmsI GCATC 4 cut(s) 34, 509, 662, 723
BpiI GAAGAC 3 cut(s) 364, 469, 630
Bpu10I CCTNAGC 1 cut(s) 189
Bsa29I ATCGAT 1 cut(s) 1002
BsaHI GRCGYC 2 cut(s) 300, 755
BsaI GGTCTC 1 cut(s) 538
BsaJI CCNNGG 4 cut(s) 103, 423, 457, 561
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 861, 883
Bse1I ACTGG 5 cut(s) 288, 442, 650, 693, 842
Bse3DI GCAATG 1 cut(s) 177
BseBI CCWGG 4 cut(s) 209, 425, 862, 1016
BseCI ATCGAT 1 cut(s) 1002
BseDI CCNNGG 4 cut(s) 103, 423, 457, 561
BseGI GGATG 3 cut(s) 130, 524, 653
BseLI CCNNNNNNNGG 3 cut(s) 190, 861, 883
BseMI GCAATG 1 cut(s) 177
BseMII CTCAG 6 cut(s) 180, 254, 741, 924, 996, 1102
BseNI ACTGG 5 cut(s) 288, 442, 650, 693, 842
BseXI GCAGC 3 cut(s) 601, 727, 1043
BsgI GTGCAG 1 cut(s) 617
BshVI ATCGAT 1 cut(s) 1002
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 1 cut(s) 339
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 3 cut(s) 190, 861, 883
BsmAI GTCTC 2 cut(s) 538, 730
BsmFI GGGAC 1 cut(s) 406
Bso31I GGTCTC 1 cut(s) 538
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 7 cut(s) 83, 219, 229, 252, 772, 880, 1003
Bsp19I CCATGG 1 cut(s) 457
BspACI CCGC 3 cut(s) 227, 1117, 1190
BspCNI CTCAG 6 cut(s) 181, 253, 740, 925, 995, 1101
BspDI ATCGAT 1 cut(s) 1002
BspPI GGATC 4 cut(s) 237, 247, 780, 998
BspTNI GGTCTC 1 cut(s) 538
BsrDI GCAATG 1 cut(s) 177
BsrI ACTGG 5 cut(s) 288, 442, 650, 693, 842
BssECI CCNNGG 4 cut(s) 103, 423, 457, 561
BssMI GATC 7 cut(s) 83, 219, 229, 252, 772, 880, 1003
BssNI GRCGYC 2 cut(s) 300, 755
BssT1I CCWWGG 1 cut(s) 457
Bst2UI CCWGG 4 cut(s) 209, 425, 862, 1016
Bst4CI ACNGT 3 cut(s) 322, 898, 1144
Bst6I CTCTTC 1 cut(s) 536
BstACI GRCGYC 2 cut(s) 300, 755
BstAPI GCANNNNNTGC 1 cut(s) 595
BstC8I GCNNGC 2 cut(s) 35, 992
BstDEI CTNAG 7 cut(s) 189, 240, 376, 727, 933, 982, 1088
BstDSI CCRYGG 2 cut(s) 103, 457
BstENI CCTNNNNNAGG 2 cut(s) 188, 881
BstF5I GGATG 3 cut(s) 130, 524, 653
BstKTI GATC 7 cut(s) 86, 222, 232, 255, 775, 883, 1006
BstMAI GTCTC 2 cut(s) 538, 730
BstMBI GATC 7 cut(s) 83, 219, 229, 252, 772, 880, 1003
BstMWI GCNNNNNNNGC 1 cut(s) 595
BstNI CCWGG 4 cut(s) 209, 425, 862, 1016
BstSCI CCNGG 4 cut(s) 207, 423, 860, 1014
BstV1I GCAGC 3 cut(s) 601, 727, 1043
BstV2I GAAGAC 3 cut(s) 364, 469, 630
BstX2I RGATCY 2 cut(s) 229, 772
BstYI RGATCY 2 cut(s) 229, 772
Bsu15I ATCGAT 1 cut(s) 1002
BsuTUI ATCGAT 1 cut(s) 1002
BtgI CCRYGG 2 cut(s) 103, 457
BtsCI GGATG 3 cut(s) 130, 524, 653
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 3 cut(s) 281, 807, 903
Cac8I GCNNGC 2 cut(s) 35, 992
CaiI CAGNNNCTG 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 146
ClaI ATCGAT 1 cut(s) 1002
Csp6I GTAC 2 cut(s) 1085, 1217
CviAII CATG 3 cut(s) 458, 719, 869
CviQI GTAC 2 cut(s) 1085, 1217
DdeI CTNAG 7 cut(s) 189, 240, 376, 727, 933, 982, 1088
DpnI GATC 7 cut(s) 85, 221, 231, 254, 774, 882, 1005
DpnII GATC 7 cut(s) 83, 219, 229, 252, 772, 880, 1003
Eam1104I CTCTTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 536
Ecl136II GAGCTC 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 16
Eco31I GGTCTC 1 cut(s) 538
Eco32I GATATC 1 cut(s) 942
Eco47I GGWCC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 14
Eco57I CTGAAG 3 cut(s) 243, 642, 810
EcoICRI GAGCTC 1 cut(s) 14
EcoNI CCTNNNNNAGG 2 cut(s) 188, 881
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 4 cut(s) 207, 423, 860, 1014
EcoRV GATATC 1 cut(s) 942
EcoT14I CCWWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 457
FaeI CATG 3 cut(s) 461, 722, 872
FalI AAGNNNNNCTT 4 cut(s) 344, 376, 947, 979
FaqI GGGAC 1 cut(s) 406
FatI CATG 3 cut(s) 457, 718, 868
FbaI TGATCA 1 cut(s) 880
FblI GTMKAC 2 cut(s) 387, 978
Fnu4HI GCNGC 4 cut(s) 590, 716, 1032, 1118
FokI GGATG 3 cut(s) 137, 531, 640
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 4 cut(s) 590, 716, 1032, 1118
FspBI CTAG 4 cut(s) 710, 762, 995, 1209
GluI GCNGC 4 cut(s) 590, 716, 1032, 1118
HapII CCGG 1 cut(s) 339
Hin1I GRCGYC 2 cut(s) 300, 755
Hin1II CATG 3 cut(s) 461, 722, 872
HincII GTYRAC 2 cut(s) 759, 979
HindII GTYRAC 2 cut(s) 759, 979
HinfI GANTC 6 cut(s) 406, 452, 570, 661, 980, 1134
HpaII CCGG 1 cut(s) 339
HphI GGTGA 2 cut(s) 298, 415
Hpy166II GTNNAC 5 cut(s) 281, 388, 759, 889, 979
Hpy188I TCNGA 8 cut(s) 262, 451, 562, 622, 730, 790, 847, 985
Hpy188III TCNNGA 6 cut(s) 242, 272, 710, 770, 955, 1007
Hpy8I GTNNAC 5 cut(s) 281, 388, 759, 889, 979
HpyAV CCTTC 3 cut(s) 267, 643, 791
HpyCH4III ACNGT 3 cut(s) 322, 898, 1144
HpyCH4IV ACGT 3 cut(s) 300, 755, 1180
HpyCH4V TGCA 7 cut(s) 351, 522, 598, 800, 868, 1031, 1103
HpyF10VI GCNNNNNNNGC 1 cut(s) 595
HpyF3I CTNAG 7 cut(s) 189, 240, 376, 727, 933, 982, 1088
HpySE526I ACGT 3 cut(s) 300, 755, 1180
Hsp92I GRCGYC 2 cut(s) 300, 755
Hsp92II CATG 3 cut(s) 461, 722, 872
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 7 cut(s) 83, 219, 229, 252, 772, 880, 1003
LmnI GCTCC 3 cut(s) 107, 822, 1078
Lsp1109I GCAGC 3 cut(s) 601, 727, 1043
LweI GCATC 4 cut(s) 34, 509, 662, 723
MaeI CTAG 4 cut(s) 710, 762, 995, 1209
MaeII ACGT 3 cut(s) 300, 755, 1180
MaeIII GTNAC 3 cut(s) 113, 403, 493
MalI GATC 7 cut(s) 85, 221, 231, 254, 774, 882, 1005
MboI GATC 7 cut(s) 83, 219, 229, 252, 772, 880, 1003
MboII GAAGA 7 cut(s) 364, 474, 553, 635, 803, 949, 1079
MfeI CAATTG 1 cut(s) 215
MflI RGATCY 2 cut(s) 229, 772
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 8 cut(s) 57, 136, 171, 215, 235, 344, 412, 443
MlyI GAGTC 2 cut(s) 400, 974
MmeI TCCRAC 1 cut(s) 1155
MnlI CCTC 7 cut(s) 184, 305, 556, 559, 628, 717, 1156
MroXI GAANNNNTTC 1 cut(s) 962
MseI TTAA 2 cut(s) 1110, 1152
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 4 cut(s) 209, 425, 862, 1016
MunI CAATTG 1 cut(s) 215
MvaI CCWGG 4 cut(s) 209, 425, 862, 1016
MwoI GCNNNNNNNGC 1 cut(s) 595
NcoI CCATGG 1 cut(s) 457
NdeII GATC 7 cut(s) 83, 219, 229, 252, 772, 880, 1003
NlaIII CATG 3 cut(s) 461, 722, 872
NmuCI GTSAC 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 962
PfeI GAWTC 4 cut(s) 452, 570, 661, 1134
PflMI CCANNNNNTGG 1 cut(s) 861
PfoI TCCNGGA 1 cut(s) 1014
PkrI GCNGC 4 cut(s) 591, 717, 1033, 1119
PleI GAGTC 2 cut(s) 400, 974
PpsI GAGTC 2 cut(s) 400, 974
PpuMI RGGWCCY 1 cut(s) 146
Psp124BI GAGCTC 1 cut(s) 16
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 4 cut(s) 207, 423, 860, 1014
PspGI CCWGG 4 cut(s) 207, 423, 860, 1014
PspPI GGNCC 1 cut(s) 146
PspPPI RGGWCCY 1 cut(s) 146
PstNI CAGNNNCTG 1 cut(s) 149
PsuI RGATCY 2 cut(s) 229, 772
RsaI GTAC 2 cut(s) 1086, 1218
RsaNI GTAC 2 cut(s) 1085, 1217
SacI GAGCTC 1 cut(s) 16
SalI GTCGAC 1 cut(s) 977
SaqAI TTAA 2 cut(s) 1110, 1152
SatI GCNGC 4 cut(s) 590, 716, 1032, 1118
Sau3AI GATC 7 cut(s) 83, 219, 229, 252, 772, 880, 1003
Sau96I GGNCC 1 cut(s) 146
ScaI AGTACT 1 cut(s) 1086
SchI GAGTC 2 cut(s) 400, 974
ScrFI CCNGG 4 cut(s) 209, 425, 862, 1016
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 4 cut(s) 34, 509, 662, 723
SinI GGWCC 1 cut(s) 146
Sse9I AATT 8 cut(s) 57, 136, 171, 215, 235, 344, 412, 443
SsiI CCGC 3 cut(s) 227, 1117, 1190
SspI AATATT 1 cut(s) 526
SspMI CTAG 4 cut(s) 710, 762, 995, 1209
SstI GAGCTC 1 cut(s) 16
StyD4I CCNGG 4 cut(s) 207, 423, 860, 1014
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 3 cut(s) 322, 898, 1144
TaiI ACGT 3 cut(s) 303, 758, 1183
TaqI TCGA 4 cut(s) 503, 978, 1002, 1127
TasI AATT 8 cut(s) 57, 136, 171, 215, 235, 344, 412, 443
TatI WGTACW 2 cut(s) 1084, 1216
TauI GCSGC 1 cut(s) 1120
TfiI GAWTC 4 cut(s) 452, 570, 661, 1134
Tru1I TTAA 2 cut(s) 1110, 1152
Tru9I TTAA 2 cut(s) 1110, 1152
TscAI CASTG 3 cut(s) 288, 807, 903
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 3 cut(s) 589, 715, 1031
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 3 cut(s) 717, 735, 843
TspGWI ACGGA 2 cut(s) 92, 216
TspRI CASTG 3 cut(s) 288, 807, 903
Van91I CCANNNNNTGG 1 cut(s) 861
VpaK11BI GGWCC 1 cut(s) 146
XagI CCTNNNNNAGG 2 cut(s) 188, 881
XapI RAATTY 2 cut(s) 235, 443
XbaI TCTAGA 1 cut(s) 709
XmiI GTMKAC 2 cut(s) 387, 978
XmnI GAANNNNTTC 1 cut(s) 962
XspI CTAG 4 cut(s) 710, 762, 995, 1209
ZraI GACGTC 2 cut(s) 301, 756
ZrmI AGTACT 1 cut(s) 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.