MD01G1170300.v1.1

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003133
Physical Location & Seq
Reverse (-)
27373168 .. 27374702
1535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1170300.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGGAATTTGCTGCTCTATGTGTTCCGGACACAATATCGATGAGGTTCATCAAGAGGGTCATCATCTTGCCGGCAGAAATGTGCACCTCATAAAGACCATGGAAAGTTGGGAGGAAAAGTTATCAGAAGCAATCAAGGATGGAAAAATTGTTGTTGCAAATTTCAGTGCATCATGGTGTAGGCCCTGTATTATGATTGCACCAGCCTACTGTGAGCTTGCAGATAAATATCCTTCTATCATATTTCTAACCTTGGATGTTGACGTTCTCGCTGAGTTGAGTACTTCATGGGATATAAAGGCCACTCCGACATTCGTTTTTCTTAAAGATGGAAGACAAGTGGACAAACTCGTCGGAGGAAACAAGCCAGAGCTCCAGAAGAAGACAGCAGCCGTCGTGGCTCAATTAGCAACCCAGTCTCCAAGATTACTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04621 ko05418 map04621 map05418
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

146

Amino Acids

16.0

Weight (kDa)

6.18

Isoelectric Point (pI)

27.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 33 - 127 1.9e-20 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
AccIII TCCGGA 1 cut(s) 26
AcsI RAATTY 2 cut(s) 6, 160
AfaI GTAC 1 cut(s) 283
AluBI AGCT 2 cut(s) 217, 373
AluI AGCT 2 cut(s) 217, 373
Alw21I GWGCWC 2 cut(s) 87, 375
Alw26I GTCTC 1 cut(s) 423
Alw44I GTGCAC 1 cut(s) 83
Aor13HI TCCGGA 1 cut(s) 26
AoxI GGCC 2 cut(s) 182, 300
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 2 cut(s) 12, 389
ApoI RAATTY 2 cut(s) 6, 160
AspS9I GGNCC 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 375
BbsI GAAGAC 2 cut(s) 340, 389
Bbv12I GWGCWC 2 cut(s) 87, 375
BbvI GCAGC 1 cut(s) 401
BccI CCATC 2 cut(s) 134, 323
BceAI ACGGC 1 cut(s) 377
BcoDI GTCTC 1 cut(s) 423
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 2 cut(s) 13, 390
BlsI GCNGC 2 cut(s) 14, 391
BmcAI AGTACT 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 183
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 1 cut(s) 179
BmuI ACTGGG 1 cut(s) 409
BpiI GAAGAC 2 cut(s) 340, 389
BpmI CTGGAG 1 cut(s) 359
Bsa29I ATCGAT 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 99, 252
BsaWI WCCGGW 1 cut(s) 26
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 403, 433
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 415
Bse8I GATNNNNATC 1 cut(s) 228
BseAI TCCGGA 1 cut(s) 26
BseCI ATCGAT 1 cut(s) 39
BseDI CCNNGG 2 cut(s) 99, 252
BseGI GGATG 2 cut(s) 145, 262
BseJI GATNNNNATC 1 cut(s) 228
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 415
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 1 cut(s) 401
BshFI GGCC 2 cut(s) 184, 302
BshVI ATCGAT 1 cut(s) 39
BsiHKAI GWGCWC 2 cut(s) 87, 375
BsiSI CCGG 2 cut(s) 27, 72
BsmAI GTCTC 1 cut(s) 423
BsnI GGCC 2 cut(s) 184, 302
Bsp1286I GDGCHC 2 cut(s) 87, 375
Bsp13I TCCGGA 1 cut(s) 26
Bsp19I CCATGG 1 cut(s) 99
BspANI GGCC 2 cut(s) 184, 302
BspCNI CTCAG 1 cut(s) 265
BspDI ATCGAT 1 cut(s) 39
BspEI TCCGGA 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 415
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 99, 252
BssT1I CCWWGG 2 cut(s) 99, 252
Bst4CI ACNGT 1 cut(s) 212
BstC8I GCNNGC 2 cut(s) 73, 219
BstDEI CTNAG 1 cut(s) 273
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 2 cut(s) 145, 262
BstMAI GTCTC 1 cut(s) 423
BstMWI GCNNNNNNNGC 2 cut(s) 398, 407
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 401
BstV2I GAAGAC 2 cut(s) 340, 389
Bsu15I ATCGAT 1 cut(s) 39
BsuRI GGCC 2 cut(s) 184, 302
BsuTUI ATCGAT 1 cut(s) 39
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 2 cut(s) 145, 262
BtsIMutI CAGTG 1 cut(s) 172
Cac8I GCNNGC 2 cut(s) 73, 219
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 183
ClaI ATCGAT 1 cut(s) 39
Csp6I GTAC 1 cut(s) 282
CviAII CATG 3 cut(s) 100, 174, 288
CviJI RGCY 8 cut(s) 184, 206, 217, 302, 367, 373, 392, 401
CviKI_1 RGCY 8 cut(s) 184, 206, 217, 302, 367, 373, 392, 401
CviQI GTAC 1 cut(s) 282
DdeI CTNAG 1 cut(s) 273
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
Ecl136II GAGCTC 1 cut(s) 373
Eco130I CCWWGG 2 cut(s) 99, 252
Eco24I GRGCYC 1 cut(s) 375
Eco53kI GAGCTC 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 373
EcoO109I RGGNCCY 1 cut(s) 183
EcoT14I CCWWGG 2 cut(s) 99, 252
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 2 cut(s) 99, 252
FaeI CATG 3 cut(s) 103, 177, 291
FaiI YATR 8 cut(s) 20, 92, 101, 175, 194, 242, 289, 296
FatI CATG 3 cut(s) 99, 173, 287
Fnu4HI GCNGC 2 cut(s) 13, 390
FokI GGATG 2 cut(s) 152, 269
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 2 cut(s) 13, 390
GluI GCNGC 2 cut(s) 13, 390
GsuI CTGGAG 1 cut(s) 359
HaeIII GGCC 2 cut(s) 184, 302
HapII CCGG 2 cut(s) 27, 72
Hin1II CATG 3 cut(s) 103, 177, 291
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HpaII CCGG 2 cut(s) 27, 72
Hpy166II GTNNAC 3 cut(s) 85, 262, 343
Hpy188I TCNGA 3 cut(s) 127, 309, 356
Hpy188III TCNNGA 3 cut(s) 27, 53, 376
Hpy8I GTNNAC 3 cut(s) 85, 262, 343
Hpy99I CGWCG 2 cut(s) 356, 398
HpyAV CCTTC 1 cut(s) 243
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 5 cut(s) 85, 158, 170, 200, 221
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 407
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 3 cut(s) 103, 177, 291
Kpn2I TCCGGA 1 cut(s) 26
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
LmnI GCTCC 1 cut(s) 378
LpnPI CCDG 7 cut(s) 40, 85, 199, 216, 381, 389, 428
Lsp1109I GCAGC 1 cut(s) 401
LweI GCATC 1 cut(s) 179
MaeII ACGT 1 cut(s) 264
MboII GAAGA 3 cut(s) 345, 391, 394
MhlI GDGCHC 2 cut(s) 87, 375
MluCI AATT 4 cut(s) 6, 147, 160, 404
MmeI TCCRAC 2 cut(s) 332, 334
MnlI CCTC 5 cut(s) 37, 49, 98, 106, 350
MroI TCCGGA 1 cut(s) 26
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 175
MspI CCGG 2 cut(s) 27, 72
MwoI GCNNNNNNNGC 2 cut(s) 398, 407
NaeI GCCGGC 1 cut(s) 73
NcoI CCATGG 1 cut(s) 99
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 3 cut(s) 103, 177, 291
PdiI GCCGGC 1 cut(s) 73
PkrI GCNGC 2 cut(s) 14, 391
Psp124BI GAGCTC 1 cut(s) 375
PspPI GGNCC 1 cut(s) 183
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
RseI CAYNNNNRTG 1 cut(s) 175
SacI GAGCTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 2 cut(s) 13, 390
Sau96I GGNCC 1 cut(s) 183
ScaI AGTACT 1 cut(s) 283
SduI GDGCHC 2 cut(s) 87, 375
SetI ASST 6 cut(s) 48, 90, 219, 254, 267, 375
SfaNI GCATC 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 175
Sse9I AATT 4 cut(s) 6, 147, 160, 404
SstI GAGCTC 1 cut(s) 375
StyI CCWWGG 2 cut(s) 99, 252
TaaI ACNGT 1 cut(s) 212
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 39
TasI AATT 4 cut(s) 6, 147, 160, 404
TatI WGTACW 1 cut(s) 281
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 172
TseI GCWGC 2 cut(s) 12, 389
TspDTI ATGAA 2 cut(s) 38, 276
TspRI CASTG 1 cut(s) 172
VneI GTGCAC 1 cut(s) 83
XapI RAATTY 2 cut(s) 6, 160
ZrmI AGTACT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.