Rh1AG382100

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003133
Physical Location & Seq
Reverse (-)
61353593 .. 61354826
1234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG382100.1

Sequence Viewer

Length: 555 bp
ATGAAGGATTTCAAAGCAGGTGAAAGCAAGCAAAAATCGCATATTTGGGTAGCCTCACTAGGGTTTGTTTCGTTCTATTTGGTACCTCAGATGTTAACTTCCTCGGGTCTGGCTGTGCACACTCAGATGGGAGCCTGTTGTTCTTCTTGTATGTTTTGTGGACATAATGGTGATGAGGGTCAACATGATGTTCATGAAGCCGGTGGGAATGTGCACTTTATAAAGACCATGGATGGTTGGGAGGCGAAGTTATCAGAAGCAATTGAGGAAGACAAACTTGTTGTTGCAAATTTTGGTGCATCATGGTGTAATCCTTCAAAAAGTATTGCACCGGCCTACTCTGAGCTTGCAGCTAAACATCCTTCAATCTTATTTCTAACCTTGGATGTCGATGACCTCGCTGAGTTGAGTACTTCATGGGATATAAAAGCCACTCCAACATTTGTTTTTCTCAAAAATGGAAGACAAGTCGATACACTTGTTGGAGGAAACAAGCTGGAGCTCCAGAAGAAAATAGCTGCTGTGGCTCAGTTAGCAACCATGTCTAGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04621 ko05418 map04621 map05418
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

184

Amino Acids

19.88

Weight (kDa)

5.76

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 84 - 173 5.7e-19 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 221
AarI CACCTGC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 8
Acc65I GGTACC 1 cut(s) 82
AccB1I GGYRCC 1 cut(s) 82
AcsI RAATTY 1 cut(s) 289
AfaI GTAC 2 cut(s) 84, 412
AfiI CCNNNNNNNGG 1 cut(s) 60
AgsI TTSAA 3 cut(s) 13, 318, 366
AluBI AGCT 5 cut(s) 346, 353, 496, 502, 518
AluI AGCT 5 cut(s) 346, 353, 496, 502, 518
Alw21I GWGCWC 3 cut(s) 120, 216, 504
Alw44I GTGCAC 2 cut(s) 116, 212
Ama87I CYCGRG 1 cut(s) 103
AoxI GGCC 1 cut(s) 333
ApaLI GTGCAC 2 cut(s) 116, 212
ApeKI GCWGC 2 cut(s) 350, 518
ApoI RAATTY 1 cut(s) 289
Asp700I GAANNNNTTC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 32, 182
AvaI CYCGRG 1 cut(s) 103
BaeGI GKGCMC 2 cut(s) 120, 216
BaeI ACNNNNGTAYC 2 cut(s) 465, 498
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 504
BbsI GAAGAC 2 cut(s) 276, 469
Bbv12I GWGCWC 3 cut(s) 120, 216, 504
BbvI GCAGC 2 cut(s) 362, 505
BccI CCATC 2 cut(s) 121, 227
BfaI CTAG 2 cut(s) 59, 546
BfuAI ACCTGC 1 cut(s) 8
BisI GCNGC 2 cut(s) 351, 519
BlsI GCNGC 2 cut(s) 352, 520
BmcAI AGTACT 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 103
BmiI GGNNCC 2 cut(s) 84, 133
BmsI GCATC 1 cut(s) 308
BpiI GAAGAC 2 cut(s) 276, 469
BpmI CTGGAG 2 cut(s) 488, 518
BsaJI CCNNGG 3 cut(s) 102, 228, 381
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 1 cut(s) 60
Bse118I RCCGGY 2 cut(s) 200, 331
BseDI CCNNGG 3 cut(s) 102, 228, 381
BseGI GGATG 3 cut(s) 238, 358, 391
BseLI CCNNNNNNNGG 1 cut(s) 60
BseMII CTCAG 5 cut(s) 101, 137, 333, 393, 542
BseSI GKGCMC 2 cut(s) 120, 216
BseXI GCAGC 2 cut(s) 362, 505
BshFI GGCC 1 cut(s) 335
BshNI GGYRCC 1 cut(s) 82
BsiHKAI GWGCWC 3 cut(s) 120, 216, 504
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 2 cut(s) 201, 332
BslI CCNNNNNNNGG 1 cut(s) 60
BsnI GGCC 1 cut(s) 335
BsoBI CYCGRG 1 cut(s) 103
Bsp1286I GDGCHC 3 cut(s) 120, 216, 504
Bsp19I CCATGG 1 cut(s) 228
BspANI GGCC 1 cut(s) 335
BspCNI CTCAG 5 cut(s) 100, 136, 334, 394, 541
BspHI TCATGA 1 cut(s) 193
BspLI GGNNCC 2 cut(s) 84, 133
BspMI ACCTGC 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 82
BsrFI RCCGGY 2 cut(s) 200, 331
BssAI RCCGGY 2 cut(s) 200, 331
BssECI CCNNGG 3 cut(s) 102, 228, 381
BssT1I CCWWGG 2 cut(s) 228, 381
BstC8I GCNNGC 2 cut(s) 29, 348
BstDEI CTNAG 5 cut(s) 87, 123, 342, 402, 528
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 3 cut(s) 238, 358, 391
BstMWI GCNNNNNNNGC 3 cut(s) 37, 524, 533
BstSLI GKGCMC 2 cut(s) 120, 216
BstV1I GCAGC 2 cut(s) 362, 505
BstV2I GAAGAC 2 cut(s) 276, 469
BsuRI GGCC 1 cut(s) 335
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 3 cut(s) 238, 358, 391
BveI ACCTGC 1 cut(s) 8
Cac8I GCNNGC 2 cut(s) 29, 348
CciI TCATGA 1 cut(s) 193
Cfr10I RCCGGY 2 cut(s) 200, 331
Csp6I GTAC 2 cut(s) 83, 411
CviAII CATG 6 cut(s) 185, 194, 229, 303, 417, 541
CviQI GTAC 2 cut(s) 83, 411
DdeI CTNAG 5 cut(s) 87, 123, 342, 402, 528
Ecl136II GAGCTC 1 cut(s) 502
Eco130I CCWWGG 2 cut(s) 228, 381
Eco24I GRGCYC 1 cut(s) 504
Eco53kI GAGCTC 1 cut(s) 502
Eco88I CYCGRG 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 502
EcoT14I CCWWGG 2 cut(s) 228, 381
EcoT38I GRGCYC 1 cut(s) 504
ErhI CCWWGG 2 cut(s) 228, 381
FaeI CATG 6 cut(s) 188, 197, 232, 306, 420, 544
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FatI CATG 6 cut(s) 184, 193, 228, 302, 416, 540
Fnu4HI GCNGC 2 cut(s) 351, 519
FokI GGATG 3 cut(s) 245, 345, 398
FriOI GRGCYC 1 cut(s) 504
Fsp4HI GCNGC 2 cut(s) 351, 519
FspBI CTAG 2 cut(s) 59, 546
GluI GCNGC 2 cut(s) 351, 519
GsuI CTGGAG 2 cut(s) 488, 518
HaeIII GGCC 1 cut(s) 335
HapII CCGG 2 cut(s) 201, 332
Hin1II CATG 6 cut(s) 188, 197, 232, 306, 420, 544
HincII GTYRAC 2 cut(s) 96, 182
HindII GTYRAC 2 cut(s) 96, 182
HpaI GTTAAC 1 cut(s) 96
HpaII CCGG 2 cut(s) 201, 332
HphI GGTGA 2 cut(s) 32, 182
Hpy166II GTNNAC 5 cut(s) 96, 118, 161, 182, 214
Hpy188I TCNGA 4 cut(s) 90, 126, 256, 343
Hpy188III TCNNGA 2 cut(s) 194, 505
Hpy8I GTNNAC 5 cut(s) 96, 118, 161, 182, 214
HpyAV CCTTC 2 cut(s) 324, 372
HpyCH4V TGCA 6 cut(s) 118, 214, 287, 299, 329, 350
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 524, 533
HpyF3I CTNAG 5 cut(s) 87, 123, 342, 402, 528
Hsp92II CATG 6 cut(s) 188, 197, 232, 306, 420, 544
KpnI GGTACC 1 cut(s) 86
KspAI GTTAAC 1 cut(s) 96
LmnI GCTCC 3 cut(s) 131, 499, 507
LpnPI CCDG 7 cut(s) 3, 95, 148, 214, 345, 482, 518
Lsp1109I GCAGC 2 cut(s) 362, 505
LweI GCATC 1 cut(s) 308
MaeI CTAG 2 cut(s) 59, 546
MboII GAAGA 4 cut(s) 135, 281, 474, 520
MfeI CAATTG 1 cut(s) 261
MhlI GDGCHC 3 cut(s) 120, 216, 504
MluCI AATT 3 cut(s) 261, 289, 550
MmeI TCCRAC 2 cut(s) 461, 463
MnlI CCTC 8 cut(s) 64, 96, 112, 169, 235, 259, 407, 479
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 1 cut(s) 95
MslI CAYNNNNRTG 3 cut(s) 125, 168, 304
MspI CCGG 2 cut(s) 201, 332
MunI CAATTG 1 cut(s) 261
MwoI GCNNNNNNNGC 3 cut(s) 37, 524, 533
NcoI CCATGG 1 cut(s) 228
NlaIII CATG 6 cut(s) 188, 197, 232, 306, 420, 544
NlaIV GGNNCC 2 cut(s) 84, 133
PagI TCATGA 1 cut(s) 193
PaqCI CACCTGC 1 cut(s) 8
PdmI GAANNNNTTC 1 cut(s) 8
PkrI GCNGC 2 cut(s) 352, 520
PsiI TTATAA 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 504
PspN4I GGNNCC 2 cut(s) 84, 133
RsaI GTAC 2 cut(s) 84, 412
RsaNI GTAC 2 cut(s) 83, 411
RseI CAYNNNNRTG 3 cut(s) 125, 168, 304
SacI GAGCTC 1 cut(s) 504
SaqAI TTAA 1 cut(s) 95
SatI GCNGC 2 cut(s) 351, 519
ScaI AGTACT 1 cut(s) 412
SduI GDGCHC 3 cut(s) 120, 216, 504
SetI ASST 9 cut(s) 22, 88, 348, 355, 383, 399, 498, 504, 520
SfaNI GCATC 1 cut(s) 308
SmiMI CAYNNNNRTG 3 cut(s) 125, 168, 304
Sse9I AATT 3 cut(s) 261, 289, 550
SspMI CTAG 2 cut(s) 59, 546
SstI GAGCTC 1 cut(s) 504
StyI CCWWGG 2 cut(s) 228, 381
TaqI TCGA 2 cut(s) 390, 471
TasI AATT 3 cut(s) 261, 289, 550
TatI WGTACW 1 cut(s) 410
Tru1I TTAA 1 cut(s) 95
Tru9I TTAA 1 cut(s) 95
TseI GCWGC 2 cut(s) 350, 518
TspDTI ATGAA 4 cut(s) 17, 182, 210, 405
VneI GTGCAC 2 cut(s) 116, 212
XapI RAATTY 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 59, 546
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.