RchiOBHm_Chr1g0372651

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003133
Physical Location & Seq
Reverse (-)
61259671 .. 61261172
1502 bp
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UTR
Exon/CDS
Intron
PRQ59662

Sequence Viewer

Length: 429 bp
ATGGGAGCCTGTTGTTCTTCTTGTATGTTTTGTGGACATAATGGTGATGAGGGTCAACATGATGTTCATGAAGCCGGTGGGAATGTGCACTTTATAAAGACCATGGATGGTTGGGAGGCGAAGTTATCAGAAGCAATTGAGGAAGACAAACTTGTTGTTGCAAATTTTGGTGCATCATGGTGTAATCCTTCTAAAAGTATTGCAGCGGCCTACTCTGAGCTTGCAGCTAAACATCCTTCAATCTTATTTCTAACCTTGGATGTCGATGACCTCGCTGAGTTGAGTACTTCATGGGATATAAAAGCCACTCCAACATTTGTTTTTCTCAAAAATGGAAGACAAGTCGATACACTTGTTGGAGGAAACAAGCAGGAGCTCCAGAAGAAAATAGCTGCTGTGGCTCAGTTAGTAACCATGTCTAGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04621 ko05418 map04621 map05418
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

142

Amino Acids

15.26

Weight (kDa)

5.07

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 42 - 131 8e-19 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 95
AciI CCGC 1 cut(s) 206
AcsI RAATTY 1 cut(s) 163
AfaI GTAC 1 cut(s) 286
AgsI TTSAA 1 cut(s) 240
AluBI AGCT 4 cut(s) 220, 227, 376, 392
AluI AGCT 4 cut(s) 220, 227, 376, 392
Alw21I GWGCWC 2 cut(s) 90, 378
Alw44I GTGCAC 1 cut(s) 86
AoxI GGCC 1 cut(s) 207
ApaLI GTGCAC 1 cut(s) 86
ApeKI GCWGC 3 cut(s) 203, 224, 392
ApoI RAATTY 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 90
BaeI ACNNNNGTAYC 2 cut(s) 339, 372
BanII GRGCYC 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 150, 343
Bbv12I GWGCWC 2 cut(s) 90, 378
BbvI GCAGC 3 cut(s) 215, 236, 379
BccI CCATC 1 cut(s) 101
BfaI CTAG 1 cut(s) 420
BisI GCNGC 4 cut(s) 204, 207, 225, 393
BlsI GCNGC 4 cut(s) 205, 208, 226, 394
BmcAI AGTACT 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 7
BmsI GCATC 1 cut(s) 182
BpiI GAAGAC 2 cut(s) 150, 343
BpmI CTGGAG 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 102, 255
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bse118I RCCGGY 1 cut(s) 74
BseDI CCNNGG 2 cut(s) 102, 255
BseGI GGATG 3 cut(s) 112, 232, 265
BseMII CTCAG 3 cut(s) 207, 267, 416
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 3 cut(s) 215, 236, 379
BshFI GGCC 1 cut(s) 209
BsiHKAI GWGCWC 2 cut(s) 90, 378
BsiSI CCGG 1 cut(s) 75
BsnI GGCC 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 90, 378
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 1 cut(s) 206
BspANI GGCC 1 cut(s) 209
BspCNI CTCAG 3 cut(s) 208, 268, 415
BspHI TCATGA 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 7
BsrFI RCCGGY 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 102, 255
BssT1I CCWWGG 2 cut(s) 102, 255
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 3 cut(s) 216, 276, 402
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 3 cut(s) 112, 232, 265
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstSLI GKGCMC 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 215, 236, 379
BstV2I GAAGAC 2 cut(s) 150, 343
BsuRI GGCC 1 cut(s) 209
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 112, 232, 265
Cac8I GCNNGC 1 cut(s) 222
CciI TCATGA 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 74
Csp6I GTAC 1 cut(s) 285
CviAII CATG 6 cut(s) 59, 68, 103, 177, 291, 415
CviJI RGCY 9 cut(s) 8, 74, 209, 220, 227, 305, 376, 392, 401
CviKI_1 RGCY 9 cut(s) 8, 74, 209, 220, 227, 305, 376, 392, 401
CviQI GTAC 1 cut(s) 285
DdeI CTNAG 3 cut(s) 216, 276, 402
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 2 cut(s) 102, 255
Eco24I GRGCYC 1 cut(s) 378
Eco53kI GAGCTC 1 cut(s) 376
EcoICRI GAGCTC 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 102, 255
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 2 cut(s) 102, 255
FaeI CATG 6 cut(s) 62, 71, 106, 180, 294, 418
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FatI CATG 6 cut(s) 58, 67, 102, 176, 290, 414
Fnu4HI GCNGC 4 cut(s) 204, 207, 225, 393
FokI GGATG 3 cut(s) 119, 219, 272
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 4 cut(s) 204, 207, 225, 393
FspBI CTAG 1 cut(s) 420
GluI GCNGC 4 cut(s) 204, 207, 225, 393
GsuI CTGGAG 1 cut(s) 362
HaeIII GGCC 1 cut(s) 209
HapII CCGG 1 cut(s) 75
Hin1II CATG 6 cut(s) 62, 71, 106, 180, 294, 418
HincII GTYRAC 1 cut(s) 56
HindII GTYRAC 1 cut(s) 56
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 3 cut(s) 35, 56, 88
Hpy188I TCNGA 2 cut(s) 130, 217
Hpy188III TCNNGA 2 cut(s) 68, 379
Hpy8I GTNNAC 3 cut(s) 35, 56, 88
HpyAV CCTTC 2 cut(s) 198, 246
HpyCH4V TGCA 5 cut(s) 88, 161, 173, 203, 224
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 3 cut(s) 216, 276, 402
Hsp92II CATG 6 cut(s) 62, 71, 106, 180, 294, 418
LmnI GCTCC 3 cut(s) 5, 373, 381
LpnPI CCDG 4 cut(s) 22, 88, 356, 392
Lsp1109I GCAGC 3 cut(s) 215, 236, 379
LweI GCATC 1 cut(s) 182
MaeI CTAG 1 cut(s) 420
MaeIII GTNAC 1 cut(s) 409
MboII GAAGA 4 cut(s) 9, 155, 348, 394
MfeI CAATTG 1 cut(s) 135
MhlI GDGCHC 2 cut(s) 90, 378
MluCI AATT 3 cut(s) 135, 163, 424
MmeI TCCRAC 2 cut(s) 335, 337
MnlI CCTC 5 cut(s) 43, 109, 133, 281, 353
MslI CAYNNNNRTG 2 cut(s) 42, 178
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 1 cut(s) 75
MunI CAATTG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 398
NcoI CCATGG 1 cut(s) 102
NlaIII CATG 6 cut(s) 62, 71, 106, 180, 294, 418
NlaIV GGNNCC 1 cut(s) 7
PagI TCATGA 1 cut(s) 67
PkrI GCNGC 4 cut(s) 205, 208, 226, 394
PsiI TTATAA 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 7
RsaI GTAC 1 cut(s) 286
RsaNI GTAC 1 cut(s) 285
RseI CAYNNNNRTG 2 cut(s) 42, 178
SacI GAGCTC 1 cut(s) 378
SatI GCNGC 4 cut(s) 204, 207, 225, 393
ScaI AGTACT 1 cut(s) 286
SduI GDGCHC 2 cut(s) 90, 378
SetI ASST 6 cut(s) 222, 229, 257, 273, 378, 394
SfaNI GCATC 1 cut(s) 182
SmiMI CAYNNNNRTG 2 cut(s) 42, 178
Sse9I AATT 3 cut(s) 135, 163, 424
SsiI CCGC 1 cut(s) 206
SspMI CTAG 1 cut(s) 420
SstI GAGCTC 1 cut(s) 378
StyI CCWWGG 2 cut(s) 102, 255
TaqI TCGA 2 cut(s) 264, 345
TasI AATT 3 cut(s) 135, 163, 424
TatI WGTACW 1 cut(s) 284
TauI GCSGC 1 cut(s) 209
TseI GCWGC 3 cut(s) 203, 224, 392
TspDTI ATGAA 3 cut(s) 56, 84, 279
VneI GTGCAC 1 cut(s) 86
XapI RAATTY 1 cut(s) 163
XspI CTAG 1 cut(s) 420
ZrmI AGTACT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.