MD07G1237200.v1.1

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003133
Physical Location & Seq
Reverse (-)
30973183 .. 30974101
919 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1237200.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGGAAGCTGCTGCTCTGTGGTAAGTTTCTTTATATGCACACAAGGATCATTTAAAACCATCCGATATAATTGGCCTTTTTCTGCATTCCATCTGAGGAAATGCTTCGATTTCTTGCAGTGTACCGGATATAATGTTGATGAGGTTCATCAAGAGGGTCATCATCTTGCCGGTGGAAATGTGCACCTTATAAAGACCATGGACGGTTGGGAGGAAAAGTTATCAGAAGCAATCAAGGATGGAAAGATTGTTGTTGCAAATTTCAGTGCATCTTGGTGTAGGCCTTGTCTTGTAATTGCACCAGCCTACTGTGAGCTTGCAGATAAATATCCTTCTACCTTATTTCTAACCGTGGATGTTGACGTTCTCGCTGAGTTGAGTACTTCATGGGATATAAAGGCCACTCCGACATTCGTTTTTCTTAAAGATGGAAGACAAGTGGACAAACTCATTGGAGGAAACAAGCCGGAGCTCCAGAAGAAGACAGCAGCCGTCGTGGCTCAGTTAGCAACCAAGTCTCCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04621 ko05418 map04621 map05418
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

176

Amino Acids

19.39

Weight (kDa)

6.93

Isoelectric Point (pI)

25.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 72 - 160 1.6e-18 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AclWI GGATC 1 cut(s) 55
AcsI RAATTY 1 cut(s) 259
AfaI GTAC 2 cut(s) 124, 382
AluBI AGCT 3 cut(s) 9, 316, 472
AluI AGCT 3 cut(s) 9, 316, 472
Alw21I GWGCWC 2 cut(s) 186, 474
Alw26I GTCTC 1 cut(s) 522
Alw44I GTGCAC 1 cut(s) 182
AlwI GGATC 1 cut(s) 55
AoxI GGCC 3 cut(s) 74, 281, 399
ApaLI GTGCAC 1 cut(s) 182
ApeKI GCWGC 3 cut(s) 9, 12, 488
ApoI RAATTY 1 cut(s) 259
Asp700I GAANNNNTTC 1 cut(s) 104
BaeGI GKGCMC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 474
BbsI GAAGAC 2 cut(s) 439, 488
Bbv12I GWGCWC 2 cut(s) 186, 474
BbvI GCAGC 1 cut(s) 500
BccI CCATC 4 cut(s) 68, 99, 233, 422
BceAI ACGGC 1 cut(s) 476
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 1 cut(s) 522
BglI GCCNNNNNGGC 1 cut(s) 497
BisI GCNGC 3 cut(s) 10, 13, 489
BlsI GCNGC 3 cut(s) 11, 14, 490
BmcAI AGTACT 1 cut(s) 382
BmsI GCATC 1 cut(s) 278
BpiI GAAGAC 2 cut(s) 439, 488
BpmI CTGGAG 1 cut(s) 458
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 2 cut(s) 198, 351
BsaWI WCCGGW 1 cut(s) 125
BsaXI ACNNNNNCTCC 3 cut(s) 203, 233, 502
Bse118I RCCGGY 1 cut(s) 170
Bse8I GATNNNNATC 1 cut(s) 327
BseDI CCNNGG 2 cut(s) 198, 351
BseGI GGATG 3 cut(s) 60, 244, 361
BseJI GATNNNNATC 1 cut(s) 327
BseMII CTCAG 3 cut(s) 86, 363, 515
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 1 cut(s) 500
BshFI GGCC 3 cut(s) 76, 283, 401
BsiHKAI GWGCWC 2 cut(s) 186, 474
BsiSI CCGG 3 cut(s) 126, 171, 467
BsmAI GTCTC 1 cut(s) 522
BsmI GAATGC 1 cut(s) 86
BsnI GGCC 3 cut(s) 76, 283, 401
Bsp1286I GDGCHC 2 cut(s) 186, 474
Bsp143I GATC 1 cut(s) 47
Bsp19I CCATGG 1 cut(s) 198
BspANI GGCC 3 cut(s) 76, 283, 401
BspCNI CTCAG 3 cut(s) 87, 364, 514
BspPI GGATC 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 198, 351
BssMI GATC 1 cut(s) 47
BssT1I CCWWGG 1 cut(s) 198
Bst4CI ACNGT 3 cut(s) 206, 311, 352
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 3 cut(s) 95, 372, 501
BstDSI CCRYGG 2 cut(s) 198, 351
BstF5I GGATG 3 cut(s) 60, 244, 361
BstKTI GATC 1 cut(s) 50
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 1 cut(s) 47
BstMWI GCNNNNNNNGC 2 cut(s) 497, 506
BstSLI GKGCMC 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 500
BstV2I GAAGAC 2 cut(s) 439, 488
BsuRI GGCC 3 cut(s) 76, 283, 401
BtgI CCRYGG 2 cut(s) 198, 351
BtsCI GGATG 3 cut(s) 60, 244, 361
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 2 cut(s) 125, 271
Cac8I GCNNGC 1 cut(s) 318
Cfr10I RCCGGY 1 cut(s) 170
Csp6I GTAC 2 cut(s) 123, 381
CviAII CATG 2 cut(s) 199, 387
CviQI GTAC 2 cut(s) 123, 381
DdeI CTNAG 3 cut(s) 95, 372, 501
DpnI GATC 1 cut(s) 49
DpnII GATC 1 cut(s) 47
DraI TTTAAA 1 cut(s) 55
Ecl136II GAGCTC 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 198
Eco147I AGGCCT 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 198
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 2 cut(s) 202, 390
FaiI YATR 8 cut(s) 35, 37, 69, 132, 191, 200, 388, 395
FatI CATG 2 cut(s) 198, 386
Fnu4HI GCNGC 3 cut(s) 10, 13, 489
FokI GGATG 3 cut(s) 47, 251, 368
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 3 cut(s) 10, 13, 489
FspBI CTAG 1 cut(s) 522
GluI GCNGC 3 cut(s) 10, 13, 489
GsuI CTGGAG 1 cut(s) 458
HaeIII GGCC 3 cut(s) 76, 283, 401
HapII CCGG 3 cut(s) 126, 171, 467
Hin1II CATG 2 cut(s) 202, 390
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HpaII CCGG 3 cut(s) 126, 171, 467
Hpy166II GTNNAC 4 cut(s) 123, 184, 361, 442
Hpy188I TCNGA 4 cut(s) 65, 96, 226, 408
Hpy188III TCNNGA 2 cut(s) 152, 475
Hpy8I GTNNAC 4 cut(s) 123, 184, 361, 442
Hpy99I CGWCG 1 cut(s) 497
HpyAV CCTTC 1 cut(s) 342
HpyCH4III ACNGT 3 cut(s) 206, 311, 352
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 8 cut(s) 39, 86, 118, 184, 257, 269, 299, 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 497, 506
HpyF3I CTNAG 3 cut(s) 95, 372, 501
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 2 cut(s) 202, 390
Kzo9I GATC 1 cut(s) 47
LmnI GCTCC 2 cut(s) 469, 477
LpnPI CCDG 5 cut(s) 139, 184, 315, 480, 488
Lsp1109I GCAGC 1 cut(s) 500
LweI GCATC 1 cut(s) 278
MaeI CTAG 1 cut(s) 522
MaeII ACGT 1 cut(s) 363
MalI GATC 1 cut(s) 49
MboI GATC 1 cut(s) 47
MboII GAAGA 3 cut(s) 444, 490, 493
MhlI GDGCHC 2 cut(s) 186, 474
MluCI AATT 3 cut(s) 70, 259, 294
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 5 cut(s) 90, 136, 148, 205, 449
MroXI GAANNNNTTC 1 cut(s) 104
MseI TTAA 2 cut(s) 54, 423
MslI CAYNNNNRTG 1 cut(s) 274
MspI CCGG 3 cut(s) 126, 171, 467
Mva1269I GAATGC 1 cut(s) 86
MwoI GCNNNNNNNGC 2 cut(s) 497, 506
NcoI CCATGG 1 cut(s) 198
NdeII GATC 1 cut(s) 47
NlaIII CATG 2 cut(s) 202, 390
PceI AGGCCT 1 cut(s) 283
PctI GAATGC 1 cut(s) 86
PdmI GAANNNNTTC 1 cut(s) 104
PkrI GCNGC 3 cut(s) 11, 14, 490
PsiI TTATAA 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 474
RsaI GTAC 2 cut(s) 124, 382
RsaNI GTAC 2 cut(s) 123, 381
RseI CAYNNNNRTG 1 cut(s) 274
SacI GAGCTC 1 cut(s) 474
SaqAI TTAA 2 cut(s) 54, 423
SatI GCNGC 3 cut(s) 10, 13, 489
Sau3AI GATC 1 cut(s) 47
ScaI AGTACT 1 cut(s) 382
SduI GDGCHC 2 cut(s) 186, 474
SetI ASST 7 cut(s) 11, 147, 189, 318, 341, 366, 474
SfaNI GCATC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 274
Sse9I AATT 3 cut(s) 70, 259, 294
SseBI AGGCCT 1 cut(s) 283
SspMI CTAG 1 cut(s) 522
SstI GAGCTC 1 cut(s) 474
StuI AGGCCT 1 cut(s) 283
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 3 cut(s) 206, 311, 352
TaiI ACGT 1 cut(s) 366
TaqI TCGA 1 cut(s) 108
TasI AATT 3 cut(s) 70, 259, 294
TatI WGTACW 1 cut(s) 380
Tru1I TTAA 2 cut(s) 54, 423
Tru9I TTAA 2 cut(s) 54, 423
TscAI CASTG 2 cut(s) 125, 271
TseI GCWGC 3 cut(s) 9, 12, 488
TspDTI ATGAA 2 cut(s) 137, 375
TspRI CASTG 2 cut(s) 125, 271
VneI GTGCAC 1 cut(s) 182
XapI RAATTY 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 1 cut(s) 522
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.