MD01G1198400.v1.1

CEN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007031
Physical Location & Seq
Reverse (-)
29614246 .. 29615530
1285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1198400.v1.1.491

Sequence Viewer

Length: 522 bp
ATGTCGAGGACGACGGAGCCATTGACGGTAGGAAGAGTGATAGGAGAGGTTGTTGACATCTTCACCCCAAGTGTGAAGCTGAATGTAGTTTACAATCCAAACAAGCAAGTTGCTAATGGACATGAGCTCATGCCTTCTGTCATTGCTGAAAAACCTCGCGTCGACATCGGCGGTGACGATATGAGGATTGCTTATACCTTAATAATGACAGACCCAGATTATCCAGGCCCCAGCGATCCATACCTGAAAGAACATCTCCACTGGATGGTCACAGACATTCCTGGGACCACTGATGTCTCATTTGGAAAAGAAACTGTGGAGTATGAAACTCCAAAGCCGGTCGTAGGCATTCACAGGTACGTGTTCTTGCTATTCAAGCAGAGAGGAAGACAAACAGTGAGGGCTCCAGCTTCCAGGGACAATTTCAACACCAGAAAATTCTCGCAGGAGAACGGTCTTGGGCTGCCTGTGGCTGCCGTCTATTTCAATGCACAACGAGAAACTGCTGCTAGAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0009908 GO:0010228 GO:0022414 GO:0032501 GO:0032502 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090567
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBP

Protein Analysis

174

Amino Acids

19.49

Weight (kDa)

8.86

Isoelectric Point (pI)

29.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PBP PF01161 63 - 161 1.1e-12 Phosphatidylethanolamine-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 265
AccI GTMKAC 1 cut(s) 162
AccII CGCG 1 cut(s) 159
AciI CCGC 1 cut(s) 171
AclWI GGATC 1 cut(s) 230
AcsI RAATTY 1 cut(s) 437
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 265, 344
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 3 cut(s) 376, 427, 487
AjnI CCWGG 3 cut(s) 223, 280, 413
AluBI AGCT 3 cut(s) 79, 127, 410
AluI AGCT 3 cut(s) 79, 127, 410
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 1 cut(s) 301
AlwI GGATC 1 cut(s) 230
AoxI GGCC 1 cut(s) 226
ApeKI GCWGC 3 cut(s) 463, 473, 506
ApoI RAATTY 1 cut(s) 437
AspS9I GGNCC 2 cut(s) 227, 285
AsuHPI GGTGA 2 cut(s) 55, 185
AvaII GGWCC 1 cut(s) 285
BanII GRGCYC 2 cut(s) 129, 406
BbsI GAAGAC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 3 cut(s) 450, 460, 493
BccI CCATC 1 cut(s) 259
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 3 cut(s) 225, 282, 415
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 1 cut(s) 510
BisI GCNGC 3 cut(s) 464, 474, 507
BlsI GCNGC 3 cut(s) 465, 475, 508
Bme1390I CCNGG 3 cut(s) 225, 282, 415
Bme18I GGWCC 1 cut(s) 285
BmgT120I GGNCC 2 cut(s) 227, 285
BmiI GGNNCC 4 cut(s) 18, 229, 286, 405
BmrFI CCNGG 3 cut(s) 225, 282, 415
BpiI GAAGAC 1 cut(s) 394
BpmI CTGGAG 1 cut(s) 390
BsaAI YACGTR 1 cut(s) 361
BsaJI CCNNGG 2 cut(s) 281, 414
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 344
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 141
BseBI CCWGG 3 cut(s) 225, 282, 415
BseDI CCNNGG 2 cut(s) 281, 414
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 2 cut(s) 265, 344
BseMI GCAATG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 266
BseXI GCAGC 3 cut(s) 450, 460, 493
BseYI CCCAGC 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 159
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 1 cut(s) 228
BsiEI CGRYCG 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 1 cut(s) 338
BslFI GGGAC 2 cut(s) 298, 431
BslI CCNNNNNNNGG 2 cut(s) 265, 344
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 2 cut(s) 298, 431
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 1 cut(s) 228
Bsp1286I GDGCHC 2 cut(s) 129, 406
Bsp143I GATC 1 cut(s) 235
BspACI CCGC 1 cut(s) 171
BspANI GGCC 1 cut(s) 228
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 4 cut(s) 18, 229, 286, 405
BspPI GGATC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 337
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 2 cut(s) 281, 414
BssMI GATC 1 cut(s) 235
Bst2UI CCWGG 3 cut(s) 225, 282, 415
Bst4CI ACNGT 4 cut(s) 28, 316, 397, 455
Bst6I CTCTTC 1 cut(s) 28
BstBAI YACGTR 1 cut(s) 361
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 1 cut(s) 159
BstKTI GATC 1 cut(s) 238
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 1 cut(s) 235
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 1 cut(s) 376
BstNI CCWGG 3 cut(s) 225, 282, 415
BstSCI CCNGG 3 cut(s) 223, 280, 413
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 3 cut(s) 450, 460, 493
BstV2I GAAGAC 1 cut(s) 394
BsuRI GGCC 1 cut(s) 228
BtsCI GGATG 1 cut(s) 270
BtsIMutI CAGTG 3 cut(s) 259, 288, 402
Cfr10I RCCGGY 1 cut(s) 337
Cfr13I GGNCC 2 cut(s) 227, 285
CseI GACGC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 358
CviAII CATG 2 cut(s) 122, 130
CviJI RGCY 9 cut(s) 19, 79, 127, 228, 337, 404, 410, 463, 473
CviKI_1 RGCY 9 cut(s) 19, 79, 127, 228, 337, 404, 410, 463, 473
CviQI GTAC 1 cut(s) 358
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 127
Eco24I GRGCYC 2 cut(s) 129, 406
Eco47I GGWCC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 127
EcoICRI GAGCTC 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 227
EcoRII CCWGG 3 cut(s) 223, 280, 413
EcoT38I GRGCYC 2 cut(s) 129, 406
FaeI CATG 2 cut(s) 125, 133
FaiI YATR 6 cut(s) 123, 131, 182, 195, 241, 324
FaqI GGGAC 2 cut(s) 298, 431
FatI CATG 2 cut(s) 121, 129
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 464, 474, 507
FokI GGATG 1 cut(s) 277
FriOI GRGCYC 2 cut(s) 129, 406
Fsp4HI GCNGC 3 cut(s) 464, 474, 507
FspBI CTAG 1 cut(s) 510
GluI GCNGC 3 cut(s) 464, 474, 507
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 1 cut(s) 228
HapII CCGG 1 cut(s) 338
HgaI GACGC 1 cut(s) 148
Hin1II CATG 2 cut(s) 125, 133
HincII GTYRAC 2 cut(s) 55, 163
HindII GTYRAC 2 cut(s) 55, 163
HpaII CCGG 1 cut(s) 338
HphI GGTGA 2 cut(s) 55, 185
Hpy166II GTNNAC 3 cut(s) 55, 91, 163
Hpy8I GTNNAC 3 cut(s) 55, 91, 163
Hpy99I CGWCG 2 cut(s) 16, 164
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 4 cut(s) 28, 316, 397, 455
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 1 cut(s) 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 376
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 2 cut(s) 125, 133
Kzo9I GATC 1 cut(s) 235
LmnI GCTCC 2 cut(s) 16, 409
Lsp1109I GCAGC 3 cut(s) 450, 460, 493
MaeI CTAG 1 cut(s) 510
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 2 cut(s) 173, 268
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MboII GAAGA 3 cut(s) 45, 52, 399
MhlI GDGCHC 2 cut(s) 129, 406
MluCI AATT 2 cut(s) 421, 437
MnlI CCTC 5 cut(s) 40, 165, 177, 377, 393
MseI TTAA 1 cut(s) 200
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 3 cut(s) 225, 282, 415
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 3 cut(s) 225, 282, 415
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 376
NdeII GATC 1 cut(s) 235
NlaIII CATG 2 cut(s) 125, 133
NlaIV GGNNCC 4 cut(s) 18, 229, 286, 405
NmuCI GTSAC 2 cut(s) 173, 268
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 1 cut(s) 348
PflMI CCANNNNNTGG 1 cut(s) 265
PkrI GCNGC 3 cut(s) 465, 475, 508
Ppu21I YACGTR 1 cut(s) 361
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 3 cut(s) 223, 280, 413
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 3 cut(s) 223, 280, 413
PspN4I GGNNCC 4 cut(s) 18, 229, 286, 405
PspPI GGNCC 2 cut(s) 227, 285
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SacI GAGCTC 1 cut(s) 129
SalI GTCGAC 1 cut(s) 161
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 3 cut(s) 464, 474, 507
Sau3AI GATC 1 cut(s) 235
Sau96I GGNCC 2 cut(s) 227, 285
ScrFI CCNGG 3 cut(s) 225, 282, 415
SduI GDGCHC 2 cut(s) 129, 406
SetI ASST 9 cut(s) 51, 81, 129, 157, 200, 246, 359, 363, 412
SinI GGWCC 1 cut(s) 285
Sse9I AATT 2 cut(s) 421, 437
SsiI CCGC 1 cut(s) 171
SspMI CTAG 1 cut(s) 510
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 223, 280, 413
TaaI ACNGT 4 cut(s) 28, 316, 397, 455
TaiI ACGT 1 cut(s) 363
TaqI TCGA 2 cut(s) 5, 162
TasI AATT 2 cut(s) 421, 437
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 3 cut(s) 266, 295, 402
TseFI GTSAC 2 cut(s) 173, 268
TseI GCWGC 3 cut(s) 463, 473, 506
Tsp45I GTSAC 2 cut(s) 173, 268
TspDTI ATGAA 1 cut(s) 339
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 3 cut(s) 266, 295, 402
Van91I CCANNNNNTGG 1 cut(s) 265
VpaK11BI GGWCC 1 cut(s) 285
XapI RAATTY 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 162
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.