Rorug06G0162700

CEN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007031
Physical Location & Seq
Reverse (-)
23199642 .. 23200049
408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0162700.1

Sequence Viewer

Length: 408 bp
ATGGAATTCAAGTTATCTCATGATAGATCAGCGATGACATTCAAGACAGCCTTTGAAAGATTAGAGGATAAAGACGCATTCTTTGTAAAGCATGTTGATCATGTCAAGGATTGTGATTGGAGGAAGTTGGTTTCTTTGCATGTCTTTCTAGAAGAATTTTATGATATCACAAACCGTGTCTCTGGTTCTAGGTATGTGACTTCTATCACATACATGGATGAATTAACACAAACAAAAGATCTTCTTGATAAGCATATGAAGAGTGAGGATGCTGATCTTCTGGCCATGGCTAAATTGATGAATGAGAAGTTTGAGAAGTACTGTAAGATTGAAGATGTTAACCAAATCTTAGTAGTTGCACTAGTGTTGGATCCTAGAAATAATTGCATTTTGTACAGTGGATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0009908 GO:0010228 GO:0022414 GO:0032501 GO:0032502 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090567
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBP

Protein Analysis

135

Amino Acids

15.88

Weight (kDa)

5.51

Isoelectric Point (pI)

27.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
hAT-like_RNase-H PF14372 61 - 127 3.1e-13 hAT-like transposase, RNase-H fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 365, 378
AcoI YGGCCR 1 cut(s) 282
AcsI RAATTY 2 cut(s) 5, 155
AfaI GTAC 2 cut(s) 320, 395
AgsI TTSAA 4 cut(s) 10, 43, 56, 332
AhlI ACTAGT 1 cut(s) 361
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 2 cut(s) 365, 378
AoxI GGCC 1 cut(s) 282
ApoI RAATTY 2 cut(s) 5, 155
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
BalI TGGCCA 1 cut(s) 284
BamHI GGATCC 1 cut(s) 370
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 1 cut(s) 184
BcuI ACTAGT 1 cut(s) 361
BfaI CTAG 5 cut(s) 149, 189, 362, 375, 406
BglII AGATCT 1 cut(s) 238
BmcAI AGTACT 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 372
BmsI GCATC 2 cut(s) 259, 392
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 285
Bse8I GATNNNNATC 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 3 cut(s) 223, 274, 407
BseJI GATNNNNATC 1 cut(s) 273
BshFI GGCC 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 184
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 1 cut(s) 284
Bsp1407I TGTACA 1 cut(s) 393
Bsp143I GATC 5 cut(s) 26, 97, 238, 274, 370
Bsp19I CCATGG 1 cut(s) 285
BspANI GGCC 1 cut(s) 284
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 1 cut(s) 372
BspPI GGATC 2 cut(s) 365, 378
BsrGI TGTACA 1 cut(s) 393
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 5 cut(s) 26, 97, 238, 274, 370
BssT1I CCWWGG 1 cut(s) 285
Bst4CI ACNGT 3 cut(s) 176, 323, 398
Bst6I CTCTTC 1 cut(s) 254
BstAUI TGTACA 1 cut(s) 393
BstDEI CTNAG 1 cut(s) 349
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 3 cut(s) 223, 274, 407
BstKTI GATC 5 cut(s) 29, 100, 241, 277, 373
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 5 cut(s) 26, 97, 238, 274, 370
BstNSI RCATGY 2 cut(s) 95, 143
BstX2I RGATCY 2 cut(s) 238, 370
BstYI RGATCY 2 cut(s) 238, 370
BsuRI GGCC 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 285
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 3 cut(s) 223, 274, 407
BtsIMutI CAGTG 1 cut(s) 403
CciI TCATGA 1 cut(s) 19
CseI GACGC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 319, 394
CviAII CATG 6 cut(s) 20, 92, 101, 140, 214, 286
CviJI RGCY 3 cut(s) 50, 284, 290
CviKI_1 RGCY 3 cut(s) 50, 284, 290
CviQI GTAC 2 cut(s) 319, 394
DdeI CTNAG 1 cut(s) 349
DpnI GATC 5 cut(s) 28, 99, 240, 276, 372
DpnII GATC 5 cut(s) 26, 97, 238, 274, 370
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
Eco130I CCWWGG 1 cut(s) 285
Eco32I GATATC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 5
EcoRV GATATC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 6 cut(s) 23, 95, 104, 143, 217, 289
FalI AAGNNNNNCTT 4 cut(s) 35, 67, 228, 260
FatI CATG 6 cut(s) 19, 91, 100, 139, 213, 285
FauNDI CATATG 1 cut(s) 255
FbaI TGATCA 1 cut(s) 97
FokI GGATG 2 cut(s) 230, 281
FspBI CTAG 5 cut(s) 149, 189, 362, 375, 406
HaeIII GGCC 1 cut(s) 284
HgaI GACGC 1 cut(s) 83
Hin1II CATG 6 cut(s) 23, 95, 104, 143, 217, 289
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HpaI GTTAAC 1 cut(s) 340
Hpy166II GTNNAC 1 cut(s) 340
Hpy188III TCNNGA 4 cut(s) 20, 43, 149, 245
Hpy8I GTNNAC 1 cut(s) 340
HpyCH4III ACNGT 3 cut(s) 176, 323, 398
HpyCH4V TGCA 3 cut(s) 139, 359, 387
HpyF3I CTNAG 1 cut(s) 349
Hsp92II CATG 6 cut(s) 23, 95, 104, 143, 217, 289
Ksp22I TGATCA 1 cut(s) 97
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 5 cut(s) 26, 97, 238, 274, 370
LpnPI CCDG 2 cut(s) 168, 266
LweI GCATC 2 cut(s) 259, 392
MaeI CTAG 5 cut(s) 149, 189, 362, 375, 406
MaeIII GTNAC 1 cut(s) 196
MalI GATC 5 cut(s) 28, 99, 240, 276, 372
MboI GATC 5 cut(s) 26, 97, 238, 274, 370
MboII GAAGA 5 cut(s) 164, 233, 269, 271, 344
MflI RGATCY 2 cut(s) 238, 370
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 5 cut(s) 5, 155, 221, 293, 382
MluNI TGGCCA 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 3 cut(s) 58, 114, 259
Mox20I TGGCCA 1 cut(s) 284
MscI TGGCCA 1 cut(s) 284
MseI TTAA 2 cut(s) 224, 339
MslI CAYNNNNRTG 1 cut(s) 212
Msp20I TGGCCA 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 77
NcoI CCATGG 1 cut(s) 285
NdeI CATATG 1 cut(s) 255
NdeII GATC 5 cut(s) 26, 97, 238, 274, 370
NlaIII CATG 6 cut(s) 23, 95, 104, 143, 217, 289
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 196
NspI RCATGY 2 cut(s) 95, 143
PagI TCATGA 1 cut(s) 19
PctI GAATGC 1 cut(s) 77
PspN4I GGNNCC 1 cut(s) 372
PsuI RGATCY 2 cut(s) 238, 370
RsaI GTAC 2 cut(s) 320, 395
RsaNI GTAC 2 cut(s) 319, 394
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 224, 339
Sau3AI GATC 5 cut(s) 26, 97, 238, 274, 370
ScaI AGTACT 1 cut(s) 320
SetI ASST 1 cut(s) 194
SfaNI GCATC 2 cut(s) 259, 392
SmiMI CAYNNNNRTG 1 cut(s) 212
SpeI ACTAGT 1 cut(s) 361
Sse9I AATT 5 cut(s) 5, 155, 221, 293, 382
SspMI CTAG 5 cut(s) 149, 189, 362, 375, 406
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 3 cut(s) 176, 323, 398
TasI AATT 5 cut(s) 5, 155, 221, 293, 382
TatI WGTACW 2 cut(s) 318, 393
Tru1I TTAA 2 cut(s) 224, 339
Tru9I TTAA 2 cut(s) 224, 339
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 3 cut(s) 234, 272, 314
TspRI CASTG 1 cut(s) 403
XapI RAATTY 2 cut(s) 5, 155
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 2 cut(s) 95, 143
XspI CTAG 5 cut(s) 149, 189, 362, 375, 406
ZrmI AGTACT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.